pycom05g24710

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003891
Physical Location & Seq
Reverse (-)
26240161 .. 26243745
3585 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g24710.5

Sequence Viewer

Length: 2751 bp
ATGAGAAAGAAAAGTAGAAGTCGGAAGCGTAAACGGAGGAGGAACTCAAGAATGGGAGCCAGAAGACGTTCAGAAGCTAATGCTGCTACTGAGGCTGATGAGAGGTTTGGGTTCATTTCAGGATCAGACTTTACATTTGAAGAGTTTCAGAAACATGCTGCTACTTTTAAGGAGAGTTACTTCGGAACAAAGGATGTCAAGGAGGGTTCAACTTGTGGTGAAACTAAAAGCAAAACATGGGAACCCTCTGTGGAGGACATCGAAGGTGAATACTGGCGAATAGTTGAGACACCAACAACAGATGAGGTTGAGGTTTACTATGGAGCTGACTTGGAAACAGGAGTATTTGGAAGTGGTTTTCCAAAGGCATCATCAACCGGAATTGATTTGGAGAAGTATGCAATGTCAGGTTGGAATCTAAACAACTTCCCACGTCTGCCTGGTTCTGCACTGTGTTTTGAAGCAAGTGATATCTCGGGAGTTCTAGTCCCATGGCTCTATGTTGGGATGTGCTTTTCTTCATTTTGTTGGCATGTTGAGGACCACCACCTCTATTCGCTAAATTATCTGCACTGGGGTGACCCAAAAGTATGGTATGGAGTCTCTGGAAGCCATGCTACGGATTTGGAACGTACAATGAAAGCGTATTTGCCTGATCTGTTCGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAGCTATCTCCTACAGTTTTAAAGTCTGAAGGTGTACCTGTGCATCGAGCCATCCAGCATTCTGGGGAATTTGTTCTTACCTTCCCGCGGGCATACCATTCTGGATTTAATTGCGGCTTCAACTGTGCTGAGGCAGTGAACGTGGCCCCTGCTGATTGGTTAGAACATGGGCAAAATGCAGTTGAGCTCTACAGTGAGCAGCATCGGAAGACATCAATTTCACACGACAAATTGTTAATGGGATCAGCTCGGGAAGCTGTACATGCCCTCTGGGAAAAATCAGTTCTTGGAAAGGAAACTACAAGAAATATGAGCTGGCAAAGTGTTTGTGGCAAAGATGGTCTACTAACCAGAGCAGTTAAGATACGAGTGCGAATGGAGGAGGAAAGACTAGATCGTCTTCCAATTTGTATGAAGTTGAAAAAGATGGAAAGAGACTTTGATTTGAACAATGAGAGAGAATGTTTTTCTTGCTTCTATGACTTGCACTTATCTGCCTCCGGCTGCAAATGCTCTCCTGACCGATTTTCATGTCTCAAACATGCAAAGCACCTTTGTTCATGTCACATCAATCATAGATATGTCCTTCAACGCCACACTATCAATGAATTAAATATGCTGGTTGAAGCATTGGAAGGGAAAGTAGAGGCCATAAAAGTATGGGCATCGAAGGACCACGGAGGAGACAGTACTGATACATATACTGCTAAGCTGGATGAAGAAAGTGGCATGCCTCATAAAAGAATCAAATCTTGTGATCCAAGGGAACCTTCACCTTGCTGCCCGGTTTCAGAAGAGAAGGTGAACATAAATGCATCTAGCAGTTCGACCAGTCAGGTTTCTTCTGCAGTAGTCCAGTCAGGGTCACAGCATGGAACTTCTAGTTTAAGTACGTCTCCCATCACTATGGATATTCAAAATGACGATCAAACTCTGGTGAAGAATGATGAAGCAAAGACGGGGATGGAATGCTTTGATCTGAATCTTAATTATATGTCTGAGGAACAAGAAAGCAGGACGATGCACACATCTGATCACTGTGATAACAAGGCTATCACAATTGAGGAGGAGACCTCTACGTTGGTGTCTAGTCAAAAGAAAGTATGTATTTCAAATGTAGCCAGAGAACCAGACATGATGAAAGTTGATGATGACTGTAATGTATCTGCTCTGACAGTTCTGAACAATGACTACCCTGCCGGTTCAAGGGACATCAGGAATAACTCTGTGAGTGAGGGCAACAAGTTGTTCGGTGTAAATATATCTGTGACTGACCAAAGCTATCCATTACAAAAGTTGATTCCTTCCGTTGAGCCTATTGATTTTGGAGCTGTTGTCTCTGGCAAGTTGTGGTGCAGTAAGCGGGCCATATATCCAAAAGGGTATAGAAGCCGAGTTAGGTTTTATAGTGTGCTTGACCCAACAAAAGTTTGTAGCTATATTTCAGAAGTTCTCGATGCTGGGCTTCTTGGTCCTCTATTCAAGGTTAGTTTGGAGGATTGCCCACGTGAGGTTTTCGCAAATGTATCGGCAGAGAAGTGCTGGGAAATGGTACTACAGAGACTACACCAAGAAATAAACAGACGGAGTAGTGTAGGGGAAAGCAGACTGCCCCATATGCAGTCATTGCAGAGCATTAATGGGCTCGAAATGTTTGGGTTTCTTTCCCAACCCATTGCTGAGGCTATTGAGGCTCTTGATCCTGGCCATCAATGCGTGGACTACTGGAATCACAGGCGCATGGTCCCGTCAATGTCTTGCAGGGTTAGTGAAATCAAGCAGCATTCATTCGAACAAAGTTGCTGTCTAGGAGAAATGGAGACGAATGTTTTTGGTGTCAGTTTGACAAAGCCGGATCAGGATTGTTCATCGGCGGAAGATCATCATTCAACTGAAGAGACGCAGTTAGTGCTACGAAGACTACTAAAGAAGGCAGATTCAGAAGAGTTAAGTACATTACAGAGAGTATTCTGCAGCAAGTCACAAAGTGCAAAATGGAGAGTGGCATTCGCATCAATGATCGAAGAGATCCAGAAACATGTAGATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

917

Amino Acids

103.26

Weight (kDa)

5.82

Isoelectric Point (pI)

54.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1095
AccI GTMKAC 1 cut(s) 1042
AccII CGCG 1 cut(s) 787
AciI CCGC 5 cut(s) 785, 787, 813, 2062, 2573
AclWI GGATC 6 cut(s) 130, 949, 1451, 2393, 2562, 2722
AcoI YGGCCR 1 cut(s) 2404
AcsI RAATTY 1 cut(s) 767
AcuI CTGAAG 2 cut(s) 747, 2613
AcvI CACGTG 1 cut(s) 2207
AdeI CACNNNGTG 1 cut(s) 2687
AfaI GTAC 7 cut(s) 634, 735, 960, 1390, 1591, 2253, 2653
AfiI CCNNNNNNNGG 4 cut(s) 619, 786, 1905, 2209
AflIII ACRYGT 1 cut(s) 2737
AjiI CACGTC 1 cut(s) 434
AjnI CCWGG 2 cut(s) 439, 2401
AjuI GAANNNNNNNTTGG 2 cut(s) 1453, 1485
AleI CACNNNNGTG 1 cut(s) 576
Alw21I GWGCWC 1 cut(s) 888
AlwI GGATC 6 cut(s) 130, 949, 1451, 2393, 2562, 2722
Ama87I CYCGRG 2 cut(s) 475, 948
AoxI GGCC 4 cut(s) 843, 1347, 2064, 2404
ApeKI GCWGC 7 cut(s) 83, 158, 898, 1203, 1479, 2479, 2673
ApoI RAATTY 1 cut(s) 767
ArsI GACNNNNNNTTYG 4 cut(s) 119, 151, 2488, 2520
AseI ATTAAT 1 cut(s) 2337
Asp700I GAANNNNTTC 3 cut(s) 67, 144, 771
AspLEI GCGC 1 cut(s) 2439
AspS9I GGNCC 6 cut(s) 541, 844, 1372, 2064, 2171, 2443
AsuC2I CCSGG 1 cut(s) 1484
AsuHPI GGTGA 6 cut(s) 230, 278, 590, 1464, 1513, 1648
AsuII TTCGAA 1 cut(s) 2490
AvaI CYCGRG 2 cut(s) 475, 948
AvaII GGWCC 4 cut(s) 541, 1372, 2171, 2443
BalI TGGCCA 1 cut(s) 2406
BanII GRGCYC 2 cut(s) 888, 2346
BarI GAAGNNNNNNTAC 2 cut(s) 601, 633
BbrPI CACGTG 1 cut(s) 2207
BbsI GAAGAC 4 cut(s) 70, 914, 1091, 2623
Bbv12I GWGCWC 1 cut(s) 888
BbvCI CCTCAGC 2 cut(s) 828, 2379
BbvI GCAGC 7 cut(s) 70, 145, 910, 1190, 1466, 2491, 2685
BccI CCATC 6 cut(s) 758, 1031, 1120, 1607, 1657, 2415
BcgI CGANNNNNNTGC 2 cut(s) 2208, 2242
BciT130I CCWGG 2 cut(s) 441, 2403
BclI TGATCA 1 cut(s) 1732
BcnI CCSGG 1 cut(s) 1484
BfaI CTAG 6 cut(s) 485, 1091, 1518, 1581, 1788, 2507
BfmI CTRYAG 5 cut(s) 711, 889, 1545, 2255, 2671
BisI GCNGC 8 cut(s) 84, 159, 814, 899, 1204, 1480, 2480, 2674
BlpI GCTNAGC 1 cut(s) 1407
BlsI GCNGC 8 cut(s) 85, 160, 815, 900, 1205, 1481, 2481, 2675
BmcAI AGTACT 1 cut(s) 1390
Bme1390I CCNGG 3 cut(s) 441, 1484, 2403
Bme18I GGWCC 4 cut(s) 541, 1372, 2171, 2443
BmeT110I CYCGRG 2 cut(s) 475, 948
BmgBI CACGTC 1 cut(s) 434
BmgT120I GGNCC 6 cut(s) 541, 844, 1372, 2064, 2171, 2443
BmiI GGNNCC 5 cut(s) 58, 243, 846, 1467, 2445
BmrFI CCNGG 3 cut(s) 441, 1484, 2403
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 8 cut(s) 377, 751, 910, 1373, 1523, 1710, 2146, 2720
BmuI ACTGGG 1 cut(s) 583
BpiI GAAGAC 4 cut(s) 70, 914, 1091, 2623
BplI GAGNNNNNCTC 2 cut(s) 1757, 1789
Bpu10I CCTNAGC 2 cut(s) 828, 2379
Bpu1102I GCTNAGC 1 cut(s) 1407
Bpu14I TTCGAA 1 cut(s) 2490
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 1484
BsaAI YACGTR 1 cut(s) 2207
BsaBI GATNNNNATC 1 cut(s) 1680
BsaI GGTCTC 1 cut(s) 1763
BsaJI CCNNGG 4 cut(s) 491, 785, 1375, 1460
BsaWI WCCGGW 1 cut(s) 377
BsaXI ACNNNNNCTCC 4 cut(s) 471, 501, 2019, 2049
Bsc4I CCNNNNNNNGG 4 cut(s) 619, 786, 1905, 2209
Bse118I RCCGGY 1 cut(s) 1898
Bse1I ACTGG 6 cut(s) 278, 578, 696, 1530, 1555, 2429
Bse3DI GCAATG 3 cut(s) 408, 2324, 2373
Bse8I GATNNNNATC 1 cut(s) 1680
BseBI CCWGG 2 cut(s) 441, 2403
BseDI CCNNGG 4 cut(s) 491, 785, 1375, 1460
BseGI GGATG 5 cut(s) 199, 513, 750, 1420, 1668
BseJI GATNNNNATC 1 cut(s) 1680
BseLI CCNNNNNNNGG 4 cut(s) 619, 786, 1905, 2209
BseMI GCAATG 3 cut(s) 408, 2324, 2373
BseMII CTCAG 5 cut(s) 81, 713, 819, 1689, 2370
BseNI ACTGG 6 cut(s) 278, 578, 696, 1530, 1555, 2429
BseRI GAGGAG 5 cut(s) 52, 1094, 1395, 1778, 1781
BseXI GCAGC 7 cut(s) 70, 145, 910, 1190, 1466, 2491, 2685
BseYI CCCAGC 2 cut(s) 2159, 2241
BsgI GTGCAG 3 cut(s) 432, 554, 2074
Bsh1236I CGCG 1 cut(s) 787
BshFI GGCC 4 cut(s) 845, 1349, 2066, 2406
BsiHKAI GWGCWC 1 cut(s) 888
BsiHKCI CYCGRG 2 cut(s) 475, 948
BsiSI CCGG 5 cut(s) 378, 1200, 1484, 1899, 2552
BslFI GGGAC 3 cut(s) 473, 1922, 2429
BslI CCNNNNNNNGG 4 cut(s) 619, 786, 1905, 2209
BsmBI CGTCTC 3 cut(s) 1599, 2513, 2591
BsmFI GGGAC 3 cut(s) 473, 1922, 2429
BsmI GAATGC 4 cut(s) 757, 1673, 2482, 2705
BsnI GGCC 4 cut(s) 845, 1349, 2066, 2406
Bso31I GGTCTC 1 cut(s) 1763
BsoBI CYCGRG 2 cut(s) 475, 948
Bsp119I TTCGAA 1 cut(s) 2490
Bsp1286I GDGCHC 2 cut(s) 888, 2346
Bsp1407I TGTACA 1 cut(s) 958
Bsp1720I GCTNAGC 1 cut(s) 1407
Bsp19I CCATGG 1 cut(s) 491
BspACI CCGC 5 cut(s) 785, 787, 813, 2062, 2573
BspANI GGCC 4 cut(s) 845, 1349, 2066, 2406
BspCNI CTCAG 5 cut(s) 82, 712, 820, 1690, 2371
BspFNI CGCG 1 cut(s) 787
BspLI GGNNCC 5 cut(s) 58, 243, 846, 1467, 2445
BspMAI CTGCAG 2 cut(s) 1549, 2675
BspPI GGATC 6 cut(s) 130, 949, 1451, 2393, 2562, 2722
BspT104I TTCGAA 1 cut(s) 2490
BspTNI GGTCTC 1 cut(s) 1763
BsrDI GCAATG 3 cut(s) 408, 2324, 2373
BsrFI RCCGGY 1 cut(s) 1898
BsrGI TGTACA 1 cut(s) 958
BsrI ACTGG 6 cut(s) 278, 578, 696, 1530, 1555, 2429
BssAI RCCGGY 1 cut(s) 1898
BssECI CCNNGG 4 cut(s) 491, 785, 1375, 1460
BssT1I CCWWGG 2 cut(s) 491, 1460
Bst2UI CCWGG 2 cut(s) 441, 2403
Bst4CI ACNGT 8 cut(s) 453, 715, 824, 893, 1388, 1739, 1856, 1876
Bst6I CTCTTC 5 cut(s) 135, 1488, 2589, 2637, 2718
BstAPI GCANNNNNTGC 2 cut(s) 2326, 2608
BstAUI TGTACA 1 cut(s) 958
BstBAI YACGTR 1 cut(s) 2207
BstBI TTCGAA 1 cut(s) 2490
BstC8I GCNNGC 4 cut(s) 789, 1016, 1430, 2064
BstDEI CTNAG 6 cut(s) 90, 699, 828, 1407, 1698, 2379
BstDSI CCRYGG 3 cut(s) 491, 785, 1375
BstEII GGTNACC 1 cut(s) 578
BstF5I GGATG 5 cut(s) 199, 513, 750, 1420, 1668
BstFNI CGCG 1 cut(s) 787
BstHHI GCGC 1 cut(s) 2439
BstNI CCWGG 2 cut(s) 441, 2403
BstNSI RCATGY 6 cut(s) 158, 536, 965, 1244, 1432, 2741
BstPI GGTNACC 1 cut(s) 578
BstSCI CCNGG 3 cut(s) 439, 1482, 2401
BstSFI CTRYAG 5 cut(s) 711, 889, 1545, 2255, 2671
BstUI CGCG 1 cut(s) 787
BstV1I GCAGC 7 cut(s) 70, 145, 910, 1190, 1466, 2491, 2685
BstV2I GAAGAC 4 cut(s) 70, 914, 1091, 2623
BstX2I RGATCY 1 cut(s) 2727
BstXI CCANNNNNNTGG 3 cut(s) 591, 761, 1606
BstYI RGATCY 1 cut(s) 2727
BsuRI GGCC 4 cut(s) 845, 1349, 2066, 2406
BtgI CCRYGG 3 cut(s) 491, 785, 1375
BtrI CACGTC 1 cut(s) 434
BtsCI GGATG 5 cut(s) 199, 513, 750, 1420, 1668
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 5 cut(s) 449, 571, 840, 898, 1735
Cac8I GCNNGC 4 cut(s) 789, 1016, 1430, 2064
CfoI GCGC 1 cut(s) 2439
Cfr10I RCCGGY 1 cut(s) 1898
Cfr13I GGNCC 6 cut(s) 541, 844, 1372, 2064, 2171, 2443
Cfr42I CCGCGG 1 cut(s) 788
CseI GACGC 1 cut(s) 2608
Csp6I GTAC 7 cut(s) 633, 734, 959, 1389, 1590, 2252, 2652
CviQI GTAC 7 cut(s) 633, 734, 959, 1389, 1590, 2252, 2652
DdeI CTNAG 6 cut(s) 90, 699, 828, 1407, 1698, 2379
DraI TTTAAA 1 cut(s) 720
DraIII CACNNNGTG 1 cut(s) 2687
DrdI GACNNNNNNGTC 1 cut(s) 1095
DseDI GACNNNNNNGTC 1 cut(s) 1095
EaeI YGGCCR 1 cut(s) 2404
Eam1104I CTCTTC 5 cut(s) 135, 1488, 2589, 2637, 2718
EarI CTCTTC 5 cut(s) 135, 1488, 2589, 2637, 2718
EciI GGCGGA 1 cut(s) 2588
Ecl136II GAGCTC 1 cut(s) 886
Eco130I CCWWGG 2 cut(s) 491, 1460
Eco24I GRGCYC 2 cut(s) 888, 2346
Eco31I GGTCTC 1 cut(s) 1763
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 4 cut(s) 541, 1372, 2171, 2443
Eco53kI GAGCTC 1 cut(s) 886
Eco57I CTGAAG 2 cut(s) 747, 2613
Eco72I CACGTG 1 cut(s) 2207
Eco88I CYCGRG 2 cut(s) 475, 948
Eco91I GGTNACC 1 cut(s) 578
EcoICRI GAGCTC 1 cut(s) 886
EcoO65I GGTNACC 1 cut(s) 578
EcoRII CCWGG 2 cut(s) 439, 2401
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 2 cut(s) 491, 1460
EcoT22I ATGCAT 1 cut(s) 1516
EcoT38I GRGCYC 2 cut(s) 888, 2346
ErhI CCWWGG 2 cut(s) 491, 1460
Esp3I CGTCTC 3 cut(s) 1599, 2513, 2591
FalI AAGNNNNNCTT 2 cut(s) 1453, 1485
FaqI GGGAC 3 cut(s) 473, 1922, 2429
FauI CCCGC 3 cut(s) 780, 792, 2055
FauNDI CATATG 1 cut(s) 2316
FbaI TGATCA 1 cut(s) 1732
FblI GTMKAC 1 cut(s) 1042
Fnu4HI GCNGC 8 cut(s) 84, 159, 814, 899, 1204, 1480, 2480, 2674
FokI GGATG 5 cut(s) 206, 520, 737, 1427, 1675
FriOI GRGCYC 2 cut(s) 888, 2346
Fsp4HI GCNGC 8 cut(s) 84, 159, 814, 899, 1204, 1480, 2480, 2674
FspBI CTAG 6 cut(s) 485, 1091, 1518, 1581, 1788, 2507
GlaI GCGC 1 cut(s) 2438
GluI GCNGC 8 cut(s) 84, 159, 814, 899, 1204, 1480, 2480, 2674
GsaI CCCAGC 2 cut(s) 2163, 2245
HaeIII GGCC 4 cut(s) 845, 1349, 2066, 2406
HapII CCGG 5 cut(s) 378, 1200, 1484, 1899, 2552
HgaI GACGC 1 cut(s) 2608
HhaI GCGC 1 cut(s) 2439
Hin6I GCGC 1 cut(s) 2437
HinP1I GCGC 1 cut(s) 2437
HinfI GANTC 7 cut(s) 415, 600, 1443, 1681, 1999, 2428, 2636
HpaII CCGG 5 cut(s) 378, 1200, 1484, 1899, 2552
HphI GGTGA 6 cut(s) 230, 278, 590, 1464, 1513, 1648
Hpy166II GTNNAC 7 cut(s) 32, 316, 734, 838, 1043, 1504, 2419
Hpy8I GTNNAC 7 cut(s) 32, 316, 734, 838, 1043, 1504, 2419
HpyCH4III ACNGT 8 cut(s) 453, 715, 824, 893, 1388, 1739, 1856, 1876
HpyCH4IV ACGT 7 cut(s) 67, 433, 631, 840, 1592, 1778, 2206
HpyF3I CTNAG 6 cut(s) 90, 699, 828, 1407, 1698, 2379
HpySE526I ACGT 7 cut(s) 67, 433, 631, 840, 1592, 1778, 2206
HspAI GCGC 1 cut(s) 2437
Ksp22I TGATCA 1 cut(s) 1732
KspI CCGCGG 1 cut(s) 788
LmnI GCTCC 3 cut(s) 56, 323, 2027
Lsp1109I GCAGC 7 cut(s) 70, 145, 910, 1190, 1466, 2491, 2685
LweI GCATC 8 cut(s) 377, 751, 910, 1373, 1523, 1710, 2146, 2720
MaeI CTAG 6 cut(s) 485, 1091, 1518, 1581, 1788, 2507
MaeII ACGT 7 cut(s) 67, 433, 631, 840, 1592, 1778, 2206
MaeIII GTNAC 7 cut(s) 176, 578, 694, 1262, 1563, 1966, 2679
MfeI CAATTG 1 cut(s) 1758
MflI RGATCY 1 cut(s) 2727
MhlI GDGCHC 2 cut(s) 888, 2346
MlsI TGGCCA 1 cut(s) 2406
MluNI TGGCCA 1 cut(s) 2406
MlyI GAGTC 1 cut(s) 609
MmeI TCCRAC 1 cut(s) 392
Mox20I TGGCCA 1 cut(s) 2406
Mph1103I ATGCAT 1 cut(s) 1516
MroXI GAANNNNTTC 3 cut(s) 67, 144, 771
MscI TGGCCA 1 cut(s) 2406
MslI CAYNNNNRTG 3 cut(s) 576, 1278, 1604
Msp20I TGGCCA 1 cut(s) 2406
MspA1I CMGCKG 1 cut(s) 787
MspI CCGG 5 cut(s) 378, 1200, 1484, 1899, 2552
MspR9I CCNGG 3 cut(s) 441, 1484, 2403
MunI CAATTG 1 cut(s) 1758
Mva1269I GAATGC 4 cut(s) 757, 1673, 2482, 2705
MvaI CCWGG 2 cut(s) 441, 2403
MvnI CGCG 1 cut(s) 787
NciI CCSGG 1 cut(s) 1484
NcoI CCATGG 1 cut(s) 491
NdeI CATATG 1 cut(s) 2316
NlaIV GGNNCC 5 cut(s) 58, 243, 846, 1467, 2445
NmeAIII GCCGAG 1 cut(s) 2117
NmuCI GTSAC 6 cut(s) 578, 694, 1262, 1563, 1966, 2679
NsiI ATGCAT 1 cut(s) 1516
NspI RCATGY 6 cut(s) 158, 536, 965, 1244, 1432, 2741
NspV TTCGAA 1 cut(s) 2490
OliI CACNNNNGTG 1 cut(s) 576
PaeI GCATGC 1 cut(s) 1432
PciI ACATGT 1 cut(s) 2737
PctI GAATGC 4 cut(s) 757, 1673, 2482, 2705
PdmI GAANNNNTTC 3 cut(s) 67, 144, 771
PfeI GAWTC 6 cut(s) 415, 1443, 1681, 1999, 2428, 2636
PkrI GCNGC 8 cut(s) 85, 160, 815, 900, 1205, 1481, 2481, 2675
PleI GAGTC 1 cut(s) 608
PmaCI CACGTG 1 cut(s) 2207
PmlI CACGTG 1 cut(s) 2207
PpsI GAGTC 1 cut(s) 608
Ppu21I YACGTR 1 cut(s) 2207
PscI ACATGT 1 cut(s) 2737
PshBI ATTAAT 1 cut(s) 2337
Psp124BI GAGCTC 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 439, 2401
PspCI CACGTG 1 cut(s) 2207
PspEI GGTNACC 1 cut(s) 578
PspFI CCCAGC 2 cut(s) 2159, 2241
PspGI CCWGG 2 cut(s) 439, 2401
PspN4I GGNNCC 5 cut(s) 58, 243, 846, 1467, 2445
PspPI GGNCC 6 cut(s) 541, 844, 1372, 2064, 2171, 2443
PsrI GAACNNNNNNTAC 2 cut(s) 1874, 1906
PstI CTGCAG 2 cut(s) 1549, 2675
PsuI RGATCY 1 cut(s) 2727
RsaI GTAC 7 cut(s) 634, 735, 960, 1390, 1591, 2253, 2653
RsaNI GTAC 7 cut(s) 633, 734, 959, 1389, 1590, 2252, 2652
RseI CAYNNNNRTG 3 cut(s) 576, 1278, 1604
SacI GAGCTC 1 cut(s) 888
SacII CCGCGG 1 cut(s) 788
SatI GCNGC 8 cut(s) 84, 159, 814, 899, 1204, 1480, 2480, 2674
Sau96I GGNCC 6 cut(s) 541, 844, 1372, 2064, 2171, 2443
ScaI AGTACT 1 cut(s) 1390
SchI GAGTC 1 cut(s) 609
ScrFI CCNGG 3 cut(s) 441, 1484, 2403
SduI GDGCHC 2 cut(s) 888, 2346
SfaNI GCATC 8 cut(s) 377, 751, 910, 1373, 1523, 1710, 2146, 2720
SfcI CTRYAG 5 cut(s) 711, 889, 1545, 2255, 2671
Sfr303I CCGCGG 1 cut(s) 788
SfuI TTCGAA 1 cut(s) 2490
SgrBI CCGCGG 1 cut(s) 788
SinI GGWCC 4 cut(s) 541, 1372, 2171, 2443
SmiMI CAYNNNNRTG 3 cut(s) 576, 1278, 1604
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
SphI GCATGC 1 cut(s) 1432
SsiI CCGC 5 cut(s) 785, 787, 813, 2062, 2573
SspMI CTAG 6 cut(s) 485, 1091, 1518, 1581, 1788, 2507
SstI GAGCTC 1 cut(s) 888
StyD4I CCNGG 3 cut(s) 439, 1482, 2401
StyI CCWWGG 2 cut(s) 491, 1460
TaaI ACNGT 8 cut(s) 453, 715, 824, 893, 1388, 1739, 1856, 1876
TaiI ACGT 7 cut(s) 70, 436, 634, 843, 1595, 1781, 2209
TaqI TCGA 9 cut(s) 261, 663, 745, 1367, 1526, 2154, 2346, 2490, 2721
TaqII GACCGA 1 cut(s) 1236
TatI WGTACW 3 cut(s) 958, 1388, 2651
TauI GCSGC 1 cut(s) 816
TfiI GAWTC 6 cut(s) 415, 1443, 1681, 1999, 2428, 2636
TscAI CASTG 5 cut(s) 456, 578, 840, 898, 1742
TseFI GTSAC 6 cut(s) 578, 694, 1262, 1563, 1966, 2679
TseI GCWGC 7 cut(s) 83, 158, 898, 1203, 1479, 2479, 2673
Tsp45I GTSAC 6 cut(s) 578, 694, 1262, 1563, 1966, 2679
TspGWI ACGGA 5 cut(s) 49, 635, 1392, 1996, 2299
TspRI CASTG 5 cut(s) 456, 578, 840, 898, 1742
VpaK11BI GGWCC 4 cut(s) 541, 1372, 2171, 2443
VspI ATTAAT 1 cut(s) 2337
XapI RAATTY 1 cut(s) 767
XceI RCATGY 6 cut(s) 158, 536, 965, 1244, 1432, 2741
XmiI GTMKAC 1 cut(s) 1042
XmnI GAANNNNTTC 3 cut(s) 67, 144, 771
XspI CTAG 6 cut(s) 485, 1091, 1518, 1581, 1788, 2507
ZrmI AGTACT 1 cut(s) 1390
Zsp2I ATGCAT 1 cut(s) 1516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.