Prupe.4G102200_v2.0.a1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0003891
Physical Location & Seq
Forward (+)
5220808 .. 5228342
7535 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G102200.1

Sequence Viewer

Length: 3204 bp
ATGCTTTCACAACAATTTCAGTCAATGGAACAGTTGAAATTAGCGGCAGAATCTCAGCAAAAAGAGGATCATTCTTTTAAGGGCAACCAGAGAAGTGATGACGCACATGAGTGCTCAGGCAGTCCTCGAAGTCGAAAGATATCAGCTAGATGGGATCCGGATGAACCATGCAGGCCTGCCATTGATGAAGCACCTGTATTTTATCCAACTATTGAGGAGTTTGAAGATACACTTGGTTACATAGCAAAGATACGCCTAGTGGCTGAATCTTATGGTATATGTCGGATTGTCCCGCCACCTTCCTGGACCCCTCCCTGCCCTCTTAAGGATAAAGAGATGTGGGAGCATGCTAAATTTTCCACACGTATTCAGCAAGTTGACTTGCTTCAAAACAGAGAAGCCATGAAAAAGAAAAGTAGAGGTCGGAAGCGAAAACGAAGAAGGCACTCAAGAATGGGAACCAAAAGACGTTCAGAAGCTAATGTTGCGTCCGAGACTGATGAGAAGTTTGGGTTCCATTCAGGATCAGACTTTACATTTGAAGAGTTTCAGAGATATGCCTATACTTTCAAGGAGAGTTACTTCAGATCAAAGGATGCTAAGGAGGGTTCAAATTCTGTTGAAACTAGAAGCAAAATATGGAAACCCTCTGTGGAAGATATTGAAGGTGAATACTGGCGAATAGTTGAGCAACCAACAGACGAGGTTGAGGTTTACTATGGAGCTGACTTGGAAACAGGAGTATTTGGAAGTGGGTTTCCTAAGGCATCGTCTATGGTAACCGAAGGTGATTCGGATCAGTATGCAATGTCTGGTTGGAATCTAAATAATTTCCCACGCCTTCCCGGTTCTGTACTGTCTTTTGAAGCAAGCGACATCTCAGGAGTTCTAGTCCCATGGCTTTATGTTGGGATGTGCTTTTCTTCATTTTGTTGGCATGTTGAGGACCACCACCTCTATTCCCTAAATTATCTGCACTGGGGTGACCCAAAAGTATGGTATGGAGTGTCTGGAAGTCGTGCTCAATCTTTGGAACGTGCAATGAGAAAGCATTTGCCGGATTTATTCGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAGCTATCTCCTTCAGTTTTAAAGTCTGAAGGTGTACCTGTGTATCGAGCTGTCCAACATTCTGGGGAGTTTATTCTTACCTTTCCGAGGGCATACCATTCTGGATTTAATTGTGGCTTTAACTGCGCCGAGGCAGTGAACGTGGCCCCTGTCGATTGGTTAGAACATGGGCAAAATGCAGTTGAGCTATACAGTGAGCAGTGTCGCAAGACATCAATCTCACACGACAAATTGTTACTGGGATCGGCACAGGAAGCTGTACAGGCTCTTTGGGAGCTATCAGTTCTTGGAAAGAAAACTACAAGAAATTTGAGCTGGCAAAATGTTTGTGGCAAAGGTGGTGTCCTCACCAAAGCAGTTAAGACACGAGTGCAAATGGAGGAGGAAAGACTAGATCGTCTTCCAATTTGTTTGAAGTTGCAAAAGATGGAAAGAGACTTTGATTTGAACGAGAGAGAATGTTTTTCTTGCTTCTATGACTTACACTTATCTGCTGCCAGCTGCAAATGCTCTCCTGATCGATTTTCATGTCTCAAACATGCAAAGCATTTTTGTTCATGTGACATCAGTCATAAATATGTCCTTCAACGCCACACTATCAGTGAATTAAATATGCTGGTTGAAGCATTGGAAGGGAGAGTAGAAGCCATGAAAGTATGGGCATCCAAGGACCCTGTAGTAGTTTCTATAGACGGTACTGATTGGCGTACCACTAAGTTGGATCAAGAAAGTAGCATGTCTCATAAGAGAGTCAAGTCTTGTAATCCAAGGGAAACTTCATCTTGTTGTCCTGTTTCGGAAGAGAAGGTCAACATAAATGCATCTTGCAGTTCGTCTAGTCAAGTTTCTTCTGCGGTTGTCCAGTCAGGGTCACAGCATGGAGCTTTTAGTTTAAGTGCATCTCGCATCACTATGGATAGGCAAAATGACGATGAAACTCTGGCCATGAATGATGAGGAAAAGATGGGGAATGAGTGCTGTTTTGATTTAAATCTTAATTATATGTCTGATGAACGTGAAAGCAGGACGATGCACATATCTGATGACTTTGATAACAAGGCTGTCACAATTGAGGAGGATGCATCTACTTCCGTGTCTAATCAAGAGAAAGTTTGTAGTTCAGATGTAGCCAGAGACCCAGACATGATGAAAGTTGATAATGGCTACCCTGCCTGTTCAAGGGACATCAGGAATAGTTGTGCGAGTGATGGCAATAAGCTGTTCGGTGTTGAACTTTGCTTACCCCATCCTTCTTCAAACAAACAGTCAATAAACTTTTCAAAAACTGAAATTGTCAAGGATTCAGGTGTAAACATATCCTTGACTGACCAAAGCTGTCAATTACAGAAGTTGAGTCCTTCGGTTGAGCCTATAGATTTTGGAGCTGTTGTGTCTGGAAAGTTGTGGTGCAGTAAGCAGGCCATATATCCAAAAGGGTATAAAAGCCGAGTTAAATTCTGTAGTGTGCTTGACCCAACAAAAGTTTGTAGCTATATTTCAGAAGTTCTCGCTGCTGGGCTTCTCGGGCCTCTATTTAAGGTTACTTTGGAAGAATGCCCTGGTGAGGCTTTTGCTAATGTATCGGCCGAGAAGTGCTGGGATATGGTACTGCAGAGACTAAACCAAGAAATAAAGAGACGGAGTAGTCTAGGGGAAAGTGGACTGCCTTCTTTGCAGCCTAGCATTAATGGCCTTGAAATGTTTGGATTTCTCTCCCAACCCATTATTGAGGCTATTGAGGCCCTCGATCCCGACCATCAATGTGTGGAGTACTGGAATTACAGGCGTATTGTCCCATTGGCGTTTGGCAATGTTAGTGAAATCAAGCAGCATTCGTTTGAATCAAGTAGAAGTCTAGGAGAAACAGATATGAAAATTTTTGGTATTACTTTGACAAGGCAGGACCGGGATAATCCATTGGTGGAAGGAGATCATCCAACCGAAGAGATGCAGTTAGTGTTGCGAAGACTATTAAAGAAGGCAGATTCCGAAGAGTTGAGGACATTACAGAGAGTATTGTGCAGTGAGTCACAAAGTTCAAAATGGAGAGTGGCATTCACATCATTGATTGAAGAGATCCAGAGAAATGTAGATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1068

Amino Acids

120.63

Weight (kDa)

5.64

Isoelectric Point (pI)

56.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1500, 2748
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 3 cut(s) 44, 293, 1958
AclWI GGATC 9 cut(s) 75, 149, 162, 532, 804, 1354, 1833, 2846, 3175
AcoI YGGCCR 2 cut(s) 2046, 2688
AcsI RAATTY 5 cut(s) 353, 613, 1411, 2558, 2979
AcuI CTGAAG 3 cut(s) 568, 1101, 1152
AfaI GTAC 7 cut(s) 855, 1140, 1365, 1801, 1813, 2712, 2876
AfiI CCNNNNNNNGG 4 cut(s) 325, 1191, 2283, 2668
AflII CTTAAG 1 cut(s) 323
AflIII ACRYGT 1 cut(s) 362
AjnI CCWGG 2 cut(s) 302, 2662
AjuI GAANNNNNNNTTGG 4 cut(s) 216, 248, 1864, 1896
AleI CACNNNNGTG 2 cut(s) 109, 981
Alw21I GWGCWC 2 cut(s) 116, 1024
Alw26I GTCTC 7 cut(s) 488, 1533, 1640, 1848, 2232, 2713, 2734
AlwI GGATC 9 cut(s) 75, 149, 162, 532, 804, 1354, 1833, 2846, 3175
Ama87I CYCGRG 1 cut(s) 2627
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 8 cut(s) 173, 1248, 2046, 2523, 2630, 2688, 2794, 2844
ApeKI GCWGC 5 cut(s) 1598, 1605, 2615, 2779, 2932
ApoI RAATTY 5 cut(s) 353, 613, 1411, 2558, 2979
ArsI GACNNNNNNTTYG 6 cut(s) 521, 553, 1431, 1463, 2354, 2386
AseI ATTAAT 1 cut(s) 2790
Asp700I GAANNNNTTC 1 cut(s) 546
AspLEI GCGC 1 cut(s) 1232
AspS9I GGNCC 7 cut(s) 306, 946, 1249, 1774, 2630, 2845, 3007
AsuC2I CCSGG 2 cut(s) 846, 3011
AsuHPI GGTGA 5 cut(s) 680, 800, 995, 1444, 2678
AvaI CYCGRG 1 cut(s) 2627
AvaII GGWCC 4 cut(s) 306, 946, 1774, 3007
AxyI CCTNAGG 1 cut(s) 762
BaeI ACNNNNGTAYC 2 cut(s) 1130, 1163
BalI TGGCCA 1 cut(s) 2048
BamHI GGATCC 1 cut(s) 154
BauI CACGAG 1 cut(s) 1470
BbsI GAAGAC 2 cut(s) 1496, 3076
Bbv12I GWGCWC 2 cut(s) 116, 1024
BbvI GCAGC 5 cut(s) 1585, 1592, 2602, 2791, 2944
BccI CCATC 6 cut(s) 144, 1525, 2062, 2306, 2358, 2868
BciT130I CCWGG 2 cut(s) 304, 2664
BcnI CCSGG 2 cut(s) 846, 3011
BcoDI GTCTC 7 cut(s) 488, 1533, 1640, 1848, 2232, 2713, 2734
BfaI CTAG 9 cut(s) 147, 257, 627, 890, 1496, 1941, 2753, 2784, 2960
BfmI CTRYAG 5 cut(s) 1779, 1791, 2475, 2563, 2714
BfrI CTTAAG 1 cut(s) 323
BisI GCNGC 6 cut(s) 45, 1599, 1606, 2616, 2780, 2933
BlsI GCNGC 6 cut(s) 46, 1600, 1607, 2617, 2781, 2934
BmcAI AGTACT 1 cut(s) 2876
Bme1390I CCNGG 4 cut(s) 304, 846, 2664, 3011
Bme18I GGWCC 4 cut(s) 306, 946, 1774, 3007
BmeT110I CYCGRG 1 cut(s) 2627
BmgT120I GGNCC 7 cut(s) 306, 946, 1249, 1774, 2630, 2845, 3007
BmiI GGNNCC 6 cut(s) 156, 308, 460, 515, 1251, 1776
BmrFI CCNGG 4 cut(s) 304, 846, 2664, 3011
BmrI ACTGGG 2 cut(s) 988, 1352
BmuI ACTGGG 2 cut(s) 988, 1352
BoxI GACNNNNGTC 1 cut(s) 1671
BpiI GAAGAC 2 cut(s) 1496, 3076
Bpu10I CCTNAGC 2 cut(s) 115, 600
BpuEI CTTGAG 1 cut(s) 433
BpuMI CCSGG 2 cut(s) 846, 3011
Bsa29I ATCGAT 1 cut(s) 1624
BsaAI YACGTR 1 cut(s) 365
BsaBI GATNNNNATC 2 cut(s) 795, 2094
BsaI GGTCTC 1 cut(s) 2232
BsaJI CCNNGG 6 cut(s) 896, 1190, 1233, 1770, 1871, 2662
BsaWI WCCGGW 1 cut(s) 157
BsaXI ACNNNNNCTCC 4 cut(s) 876, 906, 2478, 2508
Bsc4I CCNNNNNNNGG 4 cut(s) 325, 1191, 2283, 2668
Bse1I ACTGG 6 cut(s) 680, 983, 1101, 1347, 1966, 2882
Bse21I CCTNAGG 1 cut(s) 762
Bse3DI GCAATG 3 cut(s) 813, 1047, 2920
Bse8I GATNNNNATC 2 cut(s) 795, 2094
BseAI TCCGGA 1 cut(s) 157
BseBI CCWGG 2 cut(s) 304, 2664
BseCI ATCGAT 1 cut(s) 1624
BseDI CCNNGG 6 cut(s) 896, 1190, 1233, 1770, 1871, 2662
BseGI GGATG 7 cut(s) 166, 601, 918, 1766, 2188, 2350, 3037
BseJI GATNNNNATC 2 cut(s) 795, 2094
BseLI CCNNNNNNNGG 4 cut(s) 325, 1191, 2283, 2668
BseMI GCAATG 3 cut(s) 813, 1047, 2920
BseMII CTCAG 4 cut(s) 68, 129, 894, 1118
BseNI ACTGG 6 cut(s) 680, 983, 1101, 1347, 1966, 2882
BseRI GAGGAG 3 cut(s) 230, 1499, 2192
BseX3I CGGCCG 1 cut(s) 2688
BseXI GCAGC 5 cut(s) 1585, 1592, 2602, 2791, 2944
BseYI CCCAGC 2 cut(s) 2618, 2700
BsgI GTGCAG 3 cut(s) 959, 2533, 3145
Bsh1285I CGRYCG 1 cut(s) 2691
BshFI GGCC 8 cut(s) 175, 1250, 2048, 2525, 2632, 2690, 2796, 2846
BshVI ATCGAT 1 cut(s) 1624
BsiEI CGRYCG 1 cut(s) 2691
BsiHKAI GWGCWC 2 cut(s) 116, 1024
BsiHKCI CYCGRG 1 cut(s) 2627
BsiSI CCGG 4 cut(s) 158, 846, 1058, 3010
BslFI GGGAC 4 cut(s) 275, 878, 2300, 2882
BslI CCNNNNNNNGG 4 cut(s) 325, 1191, 2283, 2668
BsmAI GTCTC 7 cut(s) 488, 1533, 1640, 1848, 2232, 2713, 2734
BsmBI CGTCTC 1 cut(s) 2734
BsmFI GGGAC 4 cut(s) 275, 878, 2300, 2882
BsmI GAATGC 3 cut(s) 2663, 2935, 3158
BsnI GGCC 8 cut(s) 175, 1250, 2048, 2525, 2632, 2690, 2796, 2846
Bso31I GGTCTC 1 cut(s) 2232
BsoBI CYCGRG 1 cut(s) 2627
Bsp1286I GDGCHC 2 cut(s) 116, 1024
Bsp13I TCCGGA 1 cut(s) 157
Bsp1407I TGTACA 1 cut(s) 1363
Bsp19I CCATGG 1 cut(s) 896
BspACI CCGC 3 cut(s) 44, 293, 1958
BspANI GGCC 8 cut(s) 175, 1250, 2048, 2525, 2632, 2690, 2796, 2846
BspCNI CTCAG 4 cut(s) 67, 128, 893, 1117
BspDI ATCGAT 1 cut(s) 1624
BspEI TCCGGA 1 cut(s) 157
BspLI GGNNCC 6 cut(s) 156, 308, 460, 515, 1251, 1776
BspMAI CTGCAG 1 cut(s) 2718
BspPI GGATC 9 cut(s) 75, 149, 162, 532, 804, 1354, 1833, 2846, 3175
BspTI CTTAAG 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 2232
BsrDI GCAATG 3 cut(s) 813, 1047, 2920
BsrGI TGTACA 1 cut(s) 1363
BsrI ACTGG 6 cut(s) 680, 983, 1101, 1347, 1966, 2882
BssECI CCNNGG 6 cut(s) 896, 1190, 1233, 1770, 1871, 2662
BssSI CACGAG 1 cut(s) 1470
BssT1I CCWWGG 3 cut(s) 896, 1770, 1871
Bst2BI CACGAG 1 cut(s) 1470
Bst2UI CCWGG 2 cut(s) 304, 2664
Bst4CI ACNGT 5 cut(s) 33, 858, 1298, 1799, 2370
Bst6I CTCTTC 5 cut(s) 537, 1899, 3042, 3090, 3171
BstAFI CTTAAG 1 cut(s) 323
BstAUI TGTACA 1 cut(s) 1363
BstBAI YACGTR 1 cut(s) 365
BstC8I GCNNGC 7 cut(s) 173, 177, 348, 871, 1421, 1603, 2523
BstDEI CTNAG 7 cut(s) 54, 115, 600, 762, 880, 1104, 1818
BstDSI CCRYGG 1 cut(s) 896
BstEII GGTNACC 2 cut(s) 778, 983
BstENI CCTNNNNNAGG 2 cut(s) 1189, 2281
BstF5I GGATG 7 cut(s) 166, 601, 918, 1766, 2188, 2350, 3037
BstHHI GCGC 1 cut(s) 1232
BstMAI GTCTC 7 cut(s) 488, 1533, 1640, 1848, 2232, 2713, 2734
BstMCI CGRYCG 1 cut(s) 2691
BstMWI GCNNNNNNNGC 9 cut(s) 485, 1227, 1358, 1367, 1611, 2629, 2776, 2793, 2843
BstNI CCWGG 2 cut(s) 304, 2664
BstNSI RCATGY 4 cut(s) 350, 941, 1646, 1843
BstPAI GACNNNNGTC 1 cut(s) 1671
BstPI GGTNACC 2 cut(s) 778, 983
BstSCI CCNGG 4 cut(s) 302, 844, 2662, 3009
BstSFI CTRYAG 5 cut(s) 1779, 1791, 2475, 2563, 2714
BstV1I GCAGC 5 cut(s) 1585, 1592, 2602, 2791, 2944
BstV2I GAAGAC 2 cut(s) 1496, 3076
BstX2I RGATCY 2 cut(s) 154, 3180
BstXI CCANNNNNNTGG 4 cut(s) 303, 996, 1166, 1822
BstYI RGATCY 2 cut(s) 154, 3180
BstZI CGGCCG 1 cut(s) 2688
Bsu15I ATCGAT 1 cut(s) 1624
Bsu36I CCTNAGG 1 cut(s) 762
BsuRI GGCC 8 cut(s) 175, 1250, 2048, 2525, 2632, 2690, 2796, 2846
BsuTUI ATCGAT 1 cut(s) 1624
BtgI CCRYGG 1 cut(s) 896
BtsCI GGATG 7 cut(s) 166, 601, 918, 1766, 2188, 2350, 3037
BtsI GCAGTG 3 cut(s) 1245, 1310, 3133
BtsIMutI CAGTG 6 cut(s) 976, 1245, 1303, 1310, 1711, 3133
Cac8I GCNNGC 7 cut(s) 173, 177, 348, 871, 1421, 1603, 2523
CfoI GCGC 1 cut(s) 1232
Cfr13I GGNCC 7 cut(s) 306, 946, 1249, 1774, 2630, 2845, 3007
ClaI ATCGAT 1 cut(s) 1624
CseI GACGC 2 cut(s) 110, 477
Csp6I GTAC 7 cut(s) 854, 1139, 1364, 1800, 1812, 2711, 2875
CviQI GTAC 7 cut(s) 854, 1139, 1364, 1800, 1812, 2711, 2875
DdeI CTNAG 7 cut(s) 54, 115, 600, 762, 880, 1104, 1818
DraI TTTAAA 2 cut(s) 1125, 2094
DrdI GACNNNNNNGTC 2 cut(s) 1500, 2748
DseDI GACNNNNNNGTC 2 cut(s) 1500, 2748
EaeI YGGCCR 2 cut(s) 2046, 2688
EagI CGGCCG 1 cut(s) 2688
Eam1104I CTCTTC 5 cut(s) 537, 1899, 3042, 3090, 3171
EarI CTCTTC 5 cut(s) 537, 1899, 3042, 3090, 3171
EclXI CGGCCG 1 cut(s) 2688
Eco130I CCWWGG 3 cut(s) 896, 1770, 1871
Eco147I AGGCCT 1 cut(s) 175
Eco31I GGTCTC 1 cut(s) 2232
Eco32I GATATC 1 cut(s) 141
Eco47I GGWCC 4 cut(s) 306, 946, 1774, 3007
Eco52I CGGCCG 1 cut(s) 2688
Eco57I CTGAAG 3 cut(s) 568, 1101, 1152
Eco81I CCTNAGG 1 cut(s) 762
Eco88I CYCGRG 1 cut(s) 2627
Eco91I GGTNACC 2 cut(s) 778, 983
EcoNI CCTNNNNNAGG 2 cut(s) 1189, 2281
EcoO109I RGGNCCY 2 cut(s) 1774, 2845
EcoO65I GGTNACC 2 cut(s) 778, 983
EcoRII CCWGG 2 cut(s) 302, 2662
EcoRV GATATC 1 cut(s) 141
EcoT14I CCWWGG 3 cut(s) 896, 1770, 1871
EcoT22I ATGCAT 2 cut(s) 1927, 2188
ErhI CCWWGG 3 cut(s) 896, 1770, 1871
Esp3I CGTCTC 1 cut(s) 2734
FalI AAGNNNNNCTT 4 cut(s) 216, 248, 1864, 1896
FaqI GGGAC 4 cut(s) 275, 878, 2300, 2882
FauI CCCGC 1 cut(s) 300
Fnu4HI GCNGC 6 cut(s) 45, 1599, 1606, 2616, 2780, 2933
FokI GGATG 7 cut(s) 173, 608, 925, 1753, 2195, 2337, 3024
Fsp4HI GCNGC 6 cut(s) 45, 1599, 1606, 2616, 2780, 2933
FspBI CTAG 9 cut(s) 147, 257, 627, 890, 1496, 1941, 2753, 2784, 2960
GlaI GCGC 1 cut(s) 1231
GluI GCNGC 6 cut(s) 45, 1599, 1606, 2616, 2780, 2933
GsaI CCCAGC 2 cut(s) 2622, 2704
HaeIII GGCC 8 cut(s) 175, 1250, 2048, 2525, 2632, 2690, 2796, 2846
HapII CCGG 4 cut(s) 158, 846, 1058, 3010
HgaI GACGC 2 cut(s) 110, 477
HhaI GCGC 1 cut(s) 1232
Hin6I GCGC 1 cut(s) 1230
HinP1I GCGC 1 cut(s) 1230
HincII GTYRAC 2 cut(s) 379, 1915
HindII GTYRAC 2 cut(s) 379, 1915
HpaII CCGG 4 cut(s) 158, 846, 1058, 3010
HphI GGTGA 5 cut(s) 680, 800, 995, 1444, 2678
Hpy166II GTNNAC 7 cut(s) 379, 715, 1139, 1243, 1915, 2416, 2765
Hpy8I GTNNAC 7 cut(s) 379, 715, 1139, 1243, 1915, 2416, 2765
HpyCH4III ACNGT 5 cut(s) 33, 858, 1298, 1799, 2370
HpyCH4IV ACGT 5 cut(s) 364, 469, 1036, 1245, 2119
HpyF10VI GCNNNNNNNGC 9 cut(s) 485, 1227, 1358, 1367, 1611, 2629, 2776, 2793, 2843
HpyF3I CTNAG 7 cut(s) 54, 115, 600, 762, 880, 1104, 1818
HpySE526I ACGT 5 cut(s) 364, 469, 1036, 1245, 2119
HspAI GCGC 1 cut(s) 1230
Kpn2I TCCGGA 1 cut(s) 157
LmnI GCTCC 5 cut(s) 343, 722, 1378, 1985, 2486
Lsp1109I GCAGC 5 cut(s) 1585, 1592, 2602, 2791, 2944
MaeI CTAG 9 cut(s) 147, 257, 627, 890, 1496, 1941, 2753, 2784, 2960
MaeII ACGT 5 cut(s) 364, 469, 1036, 1245, 2119
MfeI CAATTG 1 cut(s) 2172
MflI RGATCY 2 cut(s) 154, 3180
MhlI GDGCHC 2 cut(s) 116, 1024
MlsI TGGCCA 1 cut(s) 2048
MluNI TGGCCA 1 cut(s) 2048
MlyI GAGTC 3 cut(s) 1863, 2467, 3140
MmeI TCCRAC 7 cut(s) 230, 263, 404, 797, 1183, 1803, 3065
Mox20I TGGCCA 1 cut(s) 2048
Mph1103I ATGCAT 2 cut(s) 1927, 2188
MroI TCCGGA 1 cut(s) 157
MroXI GAANNNNTTC 1 cut(s) 546
MscI TGGCCA 1 cut(s) 2048
MslI CAYNNNNRTG 5 cut(s) 109, 981, 1680, 2015, 2865
Msp20I TGGCCA 1 cut(s) 2048
MspA1I CMGCKG 1 cut(s) 1605
MspCI CTTAAG 1 cut(s) 323
MspI CCGG 4 cut(s) 158, 846, 1058, 3010
MspR9I CCNGG 4 cut(s) 304, 846, 2664, 3011
MunI CAATTG 1 cut(s) 2172
Mva1269I GAATGC 3 cut(s) 2663, 2935, 3158
MvaI CCWGG 2 cut(s) 304, 2664
MwoI GCNNNNNNNGC 9 cut(s) 485, 1227, 1358, 1367, 1611, 2629, 2776, 2793, 2843
NciI CCSGG 2 cut(s) 846, 3011
NcoI CCATGG 1 cut(s) 896
NlaIV GGNNCC 6 cut(s) 156, 308, 460, 515, 1251, 1776
NmeAIII GCCGAG 3 cut(s) 1258, 2576, 2716
NmuCI GTSAC 6 cut(s) 983, 1099, 1664, 1974, 2167, 3132
NsiI ATGCAT 2 cut(s) 1927, 2188
NspI RCATGY 4 cut(s) 350, 941, 1646, 1843
OliI CACNNNNGTG 2 cut(s) 109, 981
PaeI GCATGC 1 cut(s) 350
PceI AGGCCT 1 cut(s) 175
PctI GAATGC 3 cut(s) 2663, 2935, 3158
PdmI GAANNNNTTC 1 cut(s) 546
PfeI GAWTC 7 cut(s) 50, 266, 791, 820, 2405, 2945, 3089
PfoI TCCNGGA 1 cut(s) 302
PkrI GCNGC 6 cut(s) 46, 1600, 1607, 2617, 2781, 2934
PleI GAGTC 3 cut(s) 1862, 2466, 3139
PpsI GAGTC 3 cut(s) 1862, 2466, 3139
Ppu21I YACGTR 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 1774
PshAI GACNNNNGTC 1 cut(s) 1671
PshBI ATTAAT 1 cut(s) 2790
Psp5II RGGWCCY 1 cut(s) 1774
Psp6I CCWGG 2 cut(s) 302, 2662
PspEI GGTNACC 2 cut(s) 778, 983
PspFI CCCAGC 2 cut(s) 2618, 2700
PspGI CCWGG 2 cut(s) 302, 2662
PspN4I GGNNCC 6 cut(s) 156, 308, 460, 515, 1251, 1776
PspPI GGNCC 7 cut(s) 306, 946, 1249, 1774, 2630, 2845, 3007
PspPPI RGGWCCY 1 cut(s) 1774
PsrI GAACNNNNNNTAC 2 cut(s) 2328, 2360
PstI CTGCAG 1 cut(s) 2718
PsuI RGATCY 2 cut(s) 154, 3180
PvuII CAGCTG 1 cut(s) 1605
RsaI GTAC 7 cut(s) 855, 1140, 1365, 1801, 1813, 2712, 2876
RsaNI GTAC 7 cut(s) 854, 1139, 1364, 1800, 1812, 2711, 2875
RseI CAYNNNNRTG 5 cut(s) 109, 981, 1680, 2015, 2865
SatI GCNGC 6 cut(s) 45, 1599, 1606, 2616, 2780, 2933
Sau96I GGNCC 7 cut(s) 306, 946, 1249, 1774, 2630, 2845, 3007
ScaI AGTACT 1 cut(s) 2876
SchI GAGTC 3 cut(s) 1863, 2467, 3140
ScrFI CCNGG 4 cut(s) 304, 846, 2664, 3011
SduI GDGCHC 2 cut(s) 116, 1024
SfcI CTRYAG 5 cut(s) 1779, 1791, 2475, 2563, 2714
SinI GGWCC 4 cut(s) 306, 946, 1774, 3007
SmiI ATTTAAAT 1 cut(s) 2094
SmiMI CAYNNNNRTG 5 cut(s) 109, 981, 1680, 2015, 2865
SmlI CTYRAG 2 cut(s) 323, 448
SmoI CTYRAG 2 cut(s) 323, 448
SphI GCATGC 1 cut(s) 350
SseBI AGGCCT 1 cut(s) 175
SsiI CCGC 3 cut(s) 44, 293, 1958
SspMI CTAG 9 cut(s) 147, 257, 627, 890, 1496, 1941, 2753, 2784, 2960
StuI AGGCCT 1 cut(s) 175
StyD4I CCNGG 4 cut(s) 302, 844, 2662, 3009
StyI CCWWGG 3 cut(s) 896, 1770, 1871
SwaI ATTTAAAT 1 cut(s) 2094
TaaI ACNGT 5 cut(s) 33, 858, 1298, 1799, 2370
TaiI ACGT 5 cut(s) 367, 472, 1039, 1248, 2122
TaqI TCGA 7 cut(s) 127, 133, 1068, 1150, 1257, 1624, 2850
TatI WGTACW 3 cut(s) 853, 1363, 2874
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 7 cut(s) 50, 266, 791, 820, 2405, 2945, 3089
TscAI CASTG 6 cut(s) 983, 1245, 1303, 1310, 1711, 3133
TseFI GTSAC 6 cut(s) 983, 1099, 1664, 1974, 2167, 3132
TseI GCWGC 5 cut(s) 1598, 1605, 2615, 2779, 2932
Tsp45I GTSAC 6 cut(s) 983, 1099, 1664, 1974, 2167, 3132
TspGWI ACGGA 2 cut(s) 2185, 2758
TspRI CASTG 6 cut(s) 983, 1245, 1303, 1310, 1711, 3133
Vha464I CTTAAG 1 cut(s) 323
VpaK11BI GGWCC 4 cut(s) 306, 946, 1774, 3007
VspI ATTAAT 1 cut(s) 2790
XagI CCTNNNNNAGG 2 cut(s) 1189, 2281
XapI RAATTY 5 cut(s) 353, 613, 1411, 2558, 2979
XceI RCATGY 4 cut(s) 350, 941, 1646, 1843
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 9 cut(s) 147, 257, 627, 890, 1496, 1941, 2753, 2784, 2960
ZrmI AGTACT 1 cut(s) 2876
Zsp2I ATGCAT 2 cut(s) 1927, 2188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.