RLG00000032357

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003891
Physical Location & Seq
Forward (+)
13922428 .. 13928010
5583 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032357

Sequence Viewer

Length: 3171 bp
ATGCTGTGTACTTGTGTCACTGTGTCATTCGCGCGGGCCCCACCTTATAACTTGTGCGGTTGTAGTACTCGTCTCACCGTTTCCGTTAATACTGCGGGCGCCTTCGAGCGGGGTAAATTTCGACATGCTATTCCCAAATCACGTGGTGTTGCAAGTGTGGATAGAGCCATCCAGGATTTTAACTCTAACAAAACTGCTTTGCGGGTTCATTCTTCTAACAACAACCAGAGAAGTGATAACACAGTTGAGTGCTCTGGCAGTCCTCTAAGTCGAAAGATATCAGCTAGATGGGATCCAGATGAAGCATGCCGGCCTGTCATTGATGATGCACCTGTATTTTATCCAACTGCCGAGGAGTTCGAAAATACACTTGGTTACATAGCAAAGATTTGGCCACAGGCTGAATCTAATGGTATTTGTCGGATTGTCCCTCCACGTTCCTGGATCCCTCCTTGCCCTCTTAAAGAGAAAGACATGTGGGAAAATGCTAAATTCTCTACTCGTGTTCAGCAAGTTGACTTGCTTCAAAATAGAGAGTCCATGAAAAAGAAAACTAGAGGTCGGAAGCGAAAACGAAGAAGACACTCAAGGAGACGTGCAGAAGCTAATGCTGCTTCTGAGACCGATGAGAAGTTTGGGTTCCAGTCAGGATCAGACTTTACATTTGCAGAGTTTCAGAGACAAGCCTCTACTTTCAAGGAAAGTTACTTTGGAACACATGAATGTAAGGAGGGTTCAAATTCTGGGGGAACTAACAACAAAAGATGGGAACCCTCTGTGGAGGATATTGAAGGTGAATACTGGCGCATAGTAGAGCAACCAACAGACGAGGTTGAGGTTTACTATGGAGCTGACTTGGAAACAGGAGTATTTGGAAGTGGGTTTCCCAAGGCATCGTCTGTGGTAACTAGGAGTGAGTCAGATCATTATGCAATGTCAGGCTGGAATCTAAATAACTTGCCTCGTTTGCCTGGTTCTGTACTATGTTTTGAGGAAAGCGATATCTCGGGAGTTCTAGTCCCATGGCTCTATGTTGGGATGTGCTTTTCTTCGTTTTGTTGGCATGTTGAGGACCACCACCTCTATTCCCTAAATTATCTACACTTCGGTGACCCAAAGGTATGGTATGGAGTGCCTGGAAGCCATGCTACCTCTTTGGAACAAGCAATGAGAAAGCATTTGCCGGATTTGTTCGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAGCTATCTCCTTCAGTTTTAAAGTCTGAGGGTGTACCTGTGCATCGAGCTATCCAACATTCTGGGGAGTTTGTTCTTACCTTTCCGAGGGCATATCACTCTGGATTTAATTGTGGCTTCAATTGTGCTGAGGCAGTAAATGTCGCCCCTGTTGACTGGTTGCAACATGGCCAAACCGCAGTCGAACTTTACAGTAAGCAGTTTCGCAAGACATCACTGTCACACGACAAATTGTTACTGGGGTCAGCTCTGGAAGCCGTGCAGGTCCTTGGGGAAATATCACTTGGAACAAAATTTATTACCAACCAGAGCTGGCAAAAAGTATGTGGCAAAGATGGTGTGCTCACTAAAGCAGTTAAGAGACGAGTGGAGATGGAGGAGGAAAGACTGGATCGTCTTCCAATTTGTTGGAAGTCGCAAAAGATGGAAAGAGAATTTGATTCAAACACTGAGAGGGAATGCTTTTCTTGCTCCTATGACTTGCACTTATCTGCTGCCAGCTGCAACTGCTCTCCTGACCGATTTTCATGTCTTAAACATGCAAAGCATTTCTGTTCATGTGAAATGACTCAGAGATATGTCCTTCTCCGCTACAGTATTGAGGAATTAAATTTGCTGGTTAAAGCATTGGAAGGGGAGTTGGATGCCATTCACGTATGGGCATCTAAGGATTCTGGAGTAGTTTCTATAGACTACACTCATAAGTGCGCTGCTAAGAAGCCTAAGCTGGATCAAGATTCCAAGTCTTGCGATCCAATGGAAATTTTGCCTGGTTGCGCAGTTTCAGAAGATAAGGTTAACATGAATGGATCTTGCAGTTCCTCTAGTCCTGTTTCTTCTGCGGTAGTTCAGTCAGGATCACAGGATGGTCATAATGAAGATCAAACTGTGGCCGTGAATGCTGAAGCAAAGGTGGAGCATGATTGCAGTTTTGATTTGAATCTCAATTGTATGTCTGATGAACATGAAAGCAAGATAATTGACGTATCTGATGGCTGTGATAATAAGGCTAGTACAGTTGAGGAAGAGACATCCACATCAATGTCCAATCAAGAGAAGACTAGCATGTCCGAGGGTAATAAGTTGTTTGGTGTTGATCTTGGCTTATCATGTCCTGCTTCAAACATGCCACCAATCAGTTCTTCACAAACTGAGATTGGCGATACCTCAGCTGTGAATCCATCCATGAGAGACCAAAGCTATCGATTAAGATGTTCGAGTGTTGTTGAGCCTTTGAATTTCGGATCTGTCATGGCTGGGAAATATTGGTGCACTAAGCAGGCCATATATCCAAAAGGGTTTAGAAGCCGAGTTAATTTCTACAGTGTGCTGGACCCAACAAAACTGTGTAGTTATATATCAGAAGTTCTCGATGCTGGGCTTCTTGGACCCCTATTTAAGGTTAGTTTAGAAGAATTCCCAGATGAGGCTTTTGCAAATGTATCAGCAGACAAGTGCTGGGAGATGGTACTACATAGACTAAACAATGAAATAAGTAGACGAAGTAGTCTAGACGAAAGAGGGCCCCCTTTACAGTCTTTGCAGAGCATTAACGGGTTTGCAATGTTTGGCTTTCTCTCCCAACCTATTGTTGAGGCTATTGAGGCCCTTGATCCTGATCATCTATGCACAGAGTACTGGAATCACAGGTGTACGCAGCAGCATTCATCTGTACCAAGTTGCTCTCTAGGAGCGCCACAGACAAAGCTATTTGGTATAAACATGACAAACAAGGAGCATAATCAACTTTCTGTGGAGGGTCATCACTCAATCAATGAGATGCAGTTAGTTTTACGAAGACTCTTAGAGAAGGCGAATCCAAATCCAGAAGAGTTAAGGACGTTACATAGAATGTTATCCAGTCAGTCCGCCGAAGCAAGAGTGGCATGCGCAGAATTGATTGAAGAGATGCAGAGAAATGTAGATAACAGGAAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1057

Amino Acids

118.19

Weight (kDa)

5.79

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 111 - 144 8.3e-14 jmjN domain
JmjC PF02373 342 - 458 2.8e-46 JmjC domain, hydroxylase
zf-C5HC2 PF02928 564 - 614 2e-14 C5HC2 zinc finger
FYRN PF05964 813 - 868 3.2e-12 F/Y-rich N-terminus
FYRC PF05965 873 - 960 6.6e-21 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 48
AasI GACNNNNNNGTC 4 cut(s) 1447, 1623, 2296, 2736
Acc16I TGCGCA 2 cut(s) 2008, 3121
Acc36I ACCTGC 1 cut(s) 1483
AccB1I GGYRCC 1 cut(s) 98
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 2728
AccII CGCG 2 cut(s) 32, 34
AciI CCGC 9 cut(s) 34, 57, 95, 109, 202, 1407, 1819, 2072, 3099
AcoI YGGCCR 3 cut(s) 392, 1399, 2121
AcsI RAATTY 9 cut(s) 116, 491, 739, 1523, 1664, 1840, 1992, 2467, 2645
AcuI CTGAAG 2 cut(s) 1227, 2154
AcvI CACGTG 1 cut(s) 143
AcyI GRCGYC 1 cut(s) 99
AdeI CACNNNGTG 1 cut(s) 146
AfaI GTAC 9 cut(s) 10, 67, 981, 1266, 2245, 2700, 2867, 2884, 2904
AfiI CCNNNNNNNGG 4 cut(s) 108, 1317, 2629, 2656
AflIII ACRYGT 1 cut(s) 474
AjiI CACGTC 1 cut(s) 596
AjnI CCWGG 5 cut(s) 171, 440, 970, 1135, 1999
AjuI GAANNNNNNNTTGG 8 cut(s) 354, 386, 693, 725, 1497, 1529, 2356, 2388
AleI CACNNNNGTG 1 cut(s) 1107
Alw21I GWGCWC 3 cut(s) 254, 1575, 2504
Alw26I GTCTC 7 cut(s) 77, 586, 614, 673, 1585, 2252, 2415
Alw44I GTGCAC 1 cut(s) 2500
Ama87I CYCGRG 1 cut(s) 1006
AoxI GGCC 8 cut(s) 36, 311, 392, 1399, 2121, 2511, 2753, 2835
ApaI GGGCCC 2 cut(s) 40, 2757
ApaLI GTGCAC 1 cut(s) 2500
ApeKI GCWGC 6 cut(s) 611, 1724, 1731, 1940, 2887, 2890
ApoI RAATTY 9 cut(s) 116, 491, 739, 1523, 1664, 1840, 1992, 2467, 2645
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AspLEI GCGC 7 cut(s) 34, 101, 807, 1940, 2009, 2926, 3122
AspS9I GGNCC 9 cut(s) 36, 37, 1072, 1495, 2563, 2618, 2753, 2754, 2836
AsuHPI GGTGA 3 cut(s) 67, 806, 1121
AsuII TTCGAA 1 cut(s) 360
AvaI CYCGRG 1 cut(s) 1006
AvaII GGWCC 4 cut(s) 1072, 1495, 2563, 2618
BaeGI GKGCMC 3 cut(s) 40, 2504, 2757
BalI TGGCCA 2 cut(s) 394, 1401
BamHI GGATCC 2 cut(s) 292, 444
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 2 cut(s) 40, 2757
BarI GAAGNNNNNNTAC 2 cut(s) 1132, 1164
BauI CACGAG 1 cut(s) 501
BbrPI CACGTG 1 cut(s) 143
BbsI GAAGAC 4 cut(s) 586, 1619, 2294, 3034
Bbv12I GWGCWC 3 cut(s) 254, 1575, 2504
BbvCI CCTCAGC 2 cut(s) 1359, 2398
BbvI GCAGC 6 cut(s) 598, 1711, 1718, 1927, 2899, 2902
BceAI ACGGC 2 cut(s) 1472, 2108
BciT130I CCWGG 5 cut(s) 173, 442, 972, 1137, 2001
BclI TGATCA 1 cut(s) 2848
BcoDI GTCTC 7 cut(s) 77, 586, 614, 673, 1585, 2252, 2415
BfaI CTAG 9 cut(s) 285, 555, 909, 1016, 2055, 2241, 2292, 2741, 2918
BfmI CTRYAG 3 cut(s) 1822, 1917, 2551
BfoI RGCGCY 2 cut(s) 102, 2927
BfuAI ACCTGC 1 cut(s) 1483
BisI GCNGC 6 cut(s) 612, 1725, 1732, 1941, 2888, 2891
BlsI GCNGC 6 cut(s) 613, 1726, 1733, 1942, 2889, 2892
BmcAI AGTACT 2 cut(s) 67, 2867
Bme1390I CCNGG 5 cut(s) 173, 442, 972, 1137, 2001
Bme18I GGWCC 4 cut(s) 1072, 1495, 2563, 2618
BmeT110I CYCGRG 1 cut(s) 1006
BmgBI CACGTC 1 cut(s) 596
BmgT120I GGNCC 9 cut(s) 36, 37, 1072, 1495, 2563, 2618, 2753, 2754, 2836
BmrFI CCNGG 5 cut(s) 173, 442, 972, 1137, 2001
BmrI ACTGGG 1 cut(s) 1478
BmsI GCATC 8 cut(s) 316, 902, 1282, 1864, 1901, 2593, 3000, 3129
BmuI ACTGGG 1 cut(s) 1478
BpiI GAAGAC 4 cut(s) 586, 1619, 2294, 3034
BpmI CTGGAG 1 cut(s) 1926
Bpu10I CCTNAGC 3 cut(s) 1359, 1953, 2398
Bpu14I TTCGAA 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 571
Bsa29I ATCGAT 1 cut(s) 2434
BsaAI YACGTR 2 cut(s) 143, 1885
BsaBI GATNNNNATC 2 cut(s) 2169, 2847
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 2 cut(s) 614, 2415
BsaJI CCNNGG 6 cut(s) 351, 888, 1022, 1316, 1498, 2301
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 1317, 2629, 2656
Bse118I RCCGGY 1 cut(s) 309
Bse1I ACTGG 8 cut(s) 643, 806, 1227, 1391, 1473, 1623, 2873, 3090
Bse3DI GCAATG 3 cut(s) 939, 1173, 2799
Bse8I GATNNNNATC 2 cut(s) 2169, 2847
BseBI CCWGG 5 cut(s) 173, 442, 972, 1137, 2001
BseCI ATCGAT 1 cut(s) 2434
BseDI CCNNGG 6 cut(s) 351, 888, 1022, 1316, 1498, 2301
BseGI GGATG 6 cut(s) 168, 1044, 1879, 2101, 2261, 2411
BseJI GATNNNNATC 2 cut(s) 2169, 2847
BseLI CCNNNNNNNGG 4 cut(s) 108, 1317, 2629, 2656
BseMI GCAATG 3 cut(s) 939, 1173, 2799
BseMII CTCAG 8 cut(s) 609, 1244, 1248, 1350, 1671, 1814, 2373, 2412
BseNI ACTGG 8 cut(s) 643, 806, 1227, 1391, 1473, 1623, 2873, 3090
BseRI GAGGAG 2 cut(s) 368, 1622
BseSI GKGCMC 3 cut(s) 40, 2504, 2757
BseXI GCAGC 6 cut(s) 598, 1711, 1718, 1927, 2899, 2902
BseYI CCCAGC 3 cut(s) 2486, 2606, 2688
BsgI GTGCAG 2 cut(s) 618, 1511
Bsh1236I CGCG 2 cut(s) 32, 34
BshFI GGCC 8 cut(s) 38, 313, 394, 1401, 2123, 2513, 2755, 2837
BshNI GGYRCC 1 cut(s) 98
BshVI ATCGAT 1 cut(s) 2434
BsiHKAI GWGCWC 3 cut(s) 254, 1575, 2504
BsiHKCI CYCGRG 1 cut(s) 1006
BsiSI CCGG 2 cut(s) 310, 1184
BslFI GGGAC 2 cut(s) 413, 1004
BslI CCNNNNNNNGG 4 cut(s) 108, 1317, 2629, 2656
BsmAI GTCTC 7 cut(s) 77, 586, 614, 673, 1585, 2252, 2415
BsmBI CGTCTC 3 cut(s) 77, 586, 1585
BsmFI GGGAC 2 cut(s) 413, 1004
BsmI GAATGC 3 cut(s) 1694, 2134, 2893
BsnI GGCC 8 cut(s) 38, 313, 394, 1401, 2123, 2513, 2755, 2837
Bso31I GGTCTC 2 cut(s) 614, 2415
BsoBI CYCGRG 1 cut(s) 1006
Bsp119I TTCGAA 1 cut(s) 360
Bsp120I GGGCCC 2 cut(s) 36, 2753
Bsp1286I GDGCHC 5 cut(s) 40, 254, 1575, 2504, 2757
Bsp19I CCATGG 1 cut(s) 1022
BspACI CCGC 9 cut(s) 34, 57, 95, 109, 202, 1407, 1819, 2072, 3099
BspANI GGCC 8 cut(s) 38, 313, 394, 1401, 2123, 2513, 2755, 2837
BspCNI CTCAG 8 cut(s) 610, 1243, 1249, 1351, 1672, 1813, 2374, 2411
BspDI ATCGAT 1 cut(s) 2434
BspFNI CGCG 2 cut(s) 32, 34
BspMI ACCTGC 1 cut(s) 1483
BspT104I TTCGAA 1 cut(s) 360
BspT107I GGYRCC 1 cut(s) 98
BspTNI GGTCTC 2 cut(s) 614, 2415
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 3 cut(s) 939, 1173, 2799
BsrFI RCCGGY 1 cut(s) 309
BsrI ACTGG 8 cut(s) 643, 806, 1227, 1391, 1473, 1623, 2873, 3090
BssAI RCCGGY 1 cut(s) 309
BssECI CCNNGG 6 cut(s) 351, 888, 1022, 1316, 1498, 2301
BssNI GRCGYC 1 cut(s) 99
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 3 cut(s) 888, 1022, 1498
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 5 cut(s) 173, 442, 972, 1137, 2001
Bst6I CTCTTC 3 cut(s) 2250, 3054, 3129
BstACI GRCGYC 1 cut(s) 99
BstBAI YACGTR 2 cut(s) 143, 1885
BstBI TTCGAA 1 cut(s) 360
BstC8I GCNNGC 8 cut(s) 36, 97, 307, 311, 1544, 1729, 2511, 3118
BstDSI CCRYGG 1 cut(s) 1022
BstEII GGTNACC 1 cut(s) 1109
BstENI CCTNNNNNAGG 2 cut(s) 1315, 2627
BstF5I GGATG 6 cut(s) 168, 1044, 1879, 2101, 2261, 2411
BstFNI CGCG 2 cut(s) 32, 34
BstH2I RGCGCY 2 cut(s) 102, 2927
BstHHI GCGC 7 cut(s) 34, 101, 807, 1940, 2009, 2926, 3122
BstMAI GTCTC 7 cut(s) 77, 586, 614, 673, 1585, 2252, 2415
BstMWI GCNNNNNNNGC 8 cut(s) 611, 967, 1484, 1698, 1737, 2129, 2834, 3113
BstNI CCWGG 5 cut(s) 173, 442, 972, 1137, 2001
BstNSI RCATGY 8 cut(s) 128, 309, 478, 1067, 1772, 2299, 2359, 3120
BstPI GGTNACC 1 cut(s) 1109
BstSCI CCNGG 5 cut(s) 171, 440, 970, 1135, 1999
BstSFI CTRYAG 3 cut(s) 1822, 1917, 2551
BstSLI GKGCMC 3 cut(s) 40, 2504, 2757
BstUI CGCG 2 cut(s) 32, 34
BstV1I GCAGC 6 cut(s) 598, 1711, 1718, 1927, 2899, 2902
BstV2I GAAGAC 4 cut(s) 586, 1619, 2294, 3034
BstX2I RGATCY 4 cut(s) 292, 444, 2039, 2474
BstXI CCANNNNNNTGG 4 cut(s) 441, 1122, 1292, 1638
BstYI RGATCY 4 cut(s) 292, 444, 2039, 2474
Bsu15I ATCGAT 1 cut(s) 2434
BsuRI GGCC 8 cut(s) 38, 313, 394, 1401, 2123, 2513, 2755, 2837
BsuTUI ATCGAT 1 cut(s) 2434
BtgI CCRYGG 1 cut(s) 1022
BtrI CACGTC 1 cut(s) 596
BtsCI GGATG 6 cut(s) 168, 1044, 1879, 2101, 2261, 2411
BtsIMutI CAGTG 4 cut(s) 18, 1445, 1677, 2560
BveI ACCTGC 1 cut(s) 1483
Cac8I GCNNGC 8 cut(s) 36, 97, 307, 311, 1544, 1729, 2511, 3118
CfoI GCGC 7 cut(s) 34, 101, 807, 1940, 2009, 2926, 3122
Cfr10I RCCGGY 1 cut(s) 309
Cfr13I GGNCC 9 cut(s) 36, 37, 1072, 1495, 2563, 2618, 2753, 2754, 2836
ClaI ATCGAT 1 cut(s) 2434
Csp6I GTAC 9 cut(s) 9, 66, 980, 1265, 2244, 2699, 2866, 2883, 2903
CspCI CAANNNNNGTGG 2 cut(s) 124, 159
CviQI GTAC 9 cut(s) 9, 66, 980, 1265, 2244, 2699, 2866, 2883, 2903
DinI GGCGCC 1 cut(s) 100
DraI TTTAAA 1 cut(s) 1251
DraIII CACNNNGTG 1 cut(s) 146
DrdI GACNNNNNNGTC 4 cut(s) 1447, 1623, 2296, 2736
DseDI GACNNNNNNGTC 4 cut(s) 1447, 1623, 2296, 2736
EaeI YGGCCR 3 cut(s) 392, 1399, 2121
Eam1104I CTCTTC 3 cut(s) 2250, 3054, 3129
EarI CTCTTC 3 cut(s) 2250, 3054, 3129
EciI GGCGGA 1 cut(s) 3088
Eco130I CCWWGG 3 cut(s) 888, 1022, 1498
Eco24I GRGCYC 2 cut(s) 40, 2757
Eco31I GGTCTC 2 cut(s) 614, 2415
Eco32I GATATC 2 cut(s) 279, 1003
Eco47I GGWCC 4 cut(s) 1072, 1495, 2563, 2618
Eco57I CTGAAG 2 cut(s) 1227, 2154
Eco72I CACGTG 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 1006
Eco91I GGTNACC 1 cut(s) 1109
EcoNI CCTNNNNNAGG 2 cut(s) 1315, 2627
EcoO109I RGGNCCY 5 cut(s) 37, 1495, 2753, 2754, 2836
EcoO65I GGTNACC 1 cut(s) 1109
EcoRI GAATTC 1 cut(s) 2645
EcoRII CCWGG 5 cut(s) 171, 440, 970, 1135, 1999
EcoRV GATATC 2 cut(s) 279, 1003
EcoT14I CCWWGG 3 cut(s) 888, 1022, 1498
EcoT38I GRGCYC 2 cut(s) 40, 2757
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
ErhI CCWWGG 3 cut(s) 888, 1022, 1498
Esp3I CGTCTC 3 cut(s) 77, 586, 1585
FaqI GGGAC 2 cut(s) 413, 1004
FauI CCCGC 4 cut(s) 27, 88, 102, 195
FbaI TGATCA 1 cut(s) 2848
FblI GTMKAC 1 cut(s) 2728
Fnu4HI GCNGC 6 cut(s) 612, 1725, 1732, 1941, 2888, 2891
FokI GGATG 6 cut(s) 155, 1051, 1886, 2108, 2248, 2398
FriOI GRGCYC 2 cut(s) 40, 2757
Fsp4HI GCNGC 6 cut(s) 612, 1725, 1732, 1941, 2888, 2891
FspBI CTAG 9 cut(s) 285, 555, 909, 1016, 2055, 2241, 2292, 2741, 2918
FspI TGCGCA 2 cut(s) 2008, 3121
GlaI GCGC 7 cut(s) 33, 100, 806, 1939, 2008, 2925, 3121
GluI GCNGC 6 cut(s) 612, 1725, 1732, 1941, 2888, 2891
GsaI CCCAGC 3 cut(s) 2490, 2610, 2692
GsuI CTGGAG 1 cut(s) 1926
HaeII RGCGCY 2 cut(s) 102, 2927
HaeIII GGCC 8 cut(s) 38, 313, 394, 1401, 2123, 2513, 2755, 2837
HapII CCGG 2 cut(s) 310, 1184
HhaI GCGC 7 cut(s) 34, 101, 807, 1940, 2009, 2926, 3122
Hin1I GRCGYC 1 cut(s) 99
Hin6I GCGC 7 cut(s) 32, 99, 805, 1938, 2007, 2924, 3120
HinP1I GCGC 7 cut(s) 32, 99, 805, 1938, 2007, 2924, 3120
HincII GTYRAC 3 cut(s) 517, 1384, 2029
HindII GTYRAC 3 cut(s) 517, 1384, 2029
HpaI GTTAAC 1 cut(s) 2029
HpaII CCGG 2 cut(s) 310, 1184
HphI GGTGA 3 cut(s) 67, 806, 1121
Hpy166II GTNNAC 9 cut(s) 9, 517, 841, 1265, 1384, 2029, 2502, 2729, 2883
Hpy8I GTNNAC 9 cut(s) 9, 517, 841, 1265, 1384, 2029, 2502, 2729, 2883
HpyAV CCTTC 6 cut(s) 112, 785, 1251, 1823, 1856, 3034
HpyCH4IV ACGT 6 cut(s) 142, 436, 595, 1884, 2214, 3071
HpyF10VI GCNNNNNNNGC 8 cut(s) 611, 967, 1484, 1698, 1737, 2129, 2834, 3113
HpySE526I ACGT 6 cut(s) 142, 436, 595, 1884, 2214, 3071
Hsp92I GRCGYC 1 cut(s) 99
HspAI GCGC 7 cut(s) 32, 99, 805, 1938, 2007, 2924, 3120
KasI GGCGCC 1 cut(s) 98
KroI GCCGGC 1 cut(s) 309
KroNI GCCGGC 1 cut(s) 311
Ksp22I TGATCA 1 cut(s) 2848
KspAI GTTAAC 1 cut(s) 2029
LmnI GCTCC 5 cut(s) 848, 1706, 2146, 2921, 2965
Lsp1109I GCAGC 6 cut(s) 598, 1711, 1718, 1927, 2899, 2902
LweI GCATC 8 cut(s) 316, 902, 1282, 1864, 1901, 2593, 3000, 3129
MaeI CTAG 9 cut(s) 285, 555, 909, 1016, 2055, 2241, 2292, 2741, 2918
MaeII ACGT 6 cut(s) 142, 436, 595, 1884, 2214, 3071
MaeIII GTNAC 9 cut(s) 16, 374, 704, 904, 1109, 1225, 1449, 1464, 3072
MbiI CCGCTC 1 cut(s) 109
MfeI CAATTG 2 cut(s) 1351, 2176
MflI RGATCY 4 cut(s) 292, 444, 2039, 2474
MhlI GDGCHC 5 cut(s) 40, 254, 1575, 2504, 2757
MlsI TGGCCA 2 cut(s) 394, 1401
MluNI TGGCCA 2 cut(s) 394, 1401
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 4 cut(s) 545, 926, 1792, 3024
MmeI TCCRAC 6 cut(s) 368, 401, 542, 1309, 1619, 1851
Mox20I TGGCCA 2 cut(s) 394, 1401
MroNI GCCGGC 1 cut(s) 309
MscI TGGCCA 2 cut(s) 394, 1401
MslI CAYNNNNRTG 3 cut(s) 721, 1107, 2270
Msp20I TGGCCA 2 cut(s) 394, 1401
MspA1I CMGCKG 2 cut(s) 1731, 2402
MspI CCGG 2 cut(s) 310, 1184
MspR9I CCNGG 5 cut(s) 173, 442, 972, 1137, 2001
MunI CAATTG 2 cut(s) 1351, 2176
Mva1269I GAATGC 3 cut(s) 1694, 2134, 2893
MvaI CCWGG 5 cut(s) 173, 442, 972, 1137, 2001
MvnI CGCG 2 cut(s) 32, 34
MwoI GCNNNNNNNGC 8 cut(s) 611, 967, 1484, 1698, 1737, 2129, 2834, 3113
NaeI GCCGGC 1 cut(s) 311
NarI GGCGCC 1 cut(s) 99
NcoI CCATGG 1 cut(s) 1022
NgoMIV GCCGGC 1 cut(s) 309
NmeAIII GCCGAG 2 cut(s) 376, 2564
NmuCI GTSAC 4 cut(s) 16, 1109, 1225, 1449
NsbI TGCGCA 2 cut(s) 2008, 3121
NspI RCATGY 8 cut(s) 128, 309, 478, 1067, 1772, 2299, 2359, 3120
NspV TTCGAA 1 cut(s) 360
OliI CACNNNNGTG 1 cut(s) 1107
PaeI GCATGC 2 cut(s) 309, 3120
PciI ACATGT 1 cut(s) 474
PctI GAATGC 3 cut(s) 1694, 2134, 2893
PdiI GCCGGC 1 cut(s) 311
PfeI GAWTC 9 cut(s) 404, 946, 1670, 1901, 1967, 2170, 2407, 2872, 3046
PfoI TCCNGGA 2 cut(s) 171, 440
PkrI GCNGC 6 cut(s) 613, 1726, 1733, 1942, 2889, 2892
PleI GAGTC 4 cut(s) 544, 925, 1792, 3024
PluTI GGCGCC 1 cut(s) 102
PmaCI CACGTG 1 cut(s) 143
PmlI CACGTG 1 cut(s) 143
PpsI GAGTC 4 cut(s) 544, 925, 1792, 3024
Ppu21I YACGTR 2 cut(s) 143, 1885
PpuMI RGGWCCY 1 cut(s) 1495
PscI ACATGT 1 cut(s) 474
PsiI TTATAA 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 1495
Psp6I CCWGG 5 cut(s) 171, 440, 970, 1135, 1999
PspCI CACGTG 1 cut(s) 143
PspEI GGTNACC 1 cut(s) 1109
PspFI CCCAGC 3 cut(s) 2486, 2606, 2688
PspGI CCWGG 5 cut(s) 171, 440, 970, 1135, 1999
PspOMI GGGCCC 2 cut(s) 36, 2753
PspPI GGNCC 9 cut(s) 36, 37, 1072, 1495, 2563, 2618, 2753, 2754, 2836
PspPPI RGGWCCY 1 cut(s) 1495
PsrI GAACNNNNNNTAC 2 cut(s) 718, 750
PsuI RGATCY 4 cut(s) 292, 444, 2039, 2474
PvuII CAGCTG 2 cut(s) 1731, 2402
RsaI GTAC 9 cut(s) 10, 67, 981, 1266, 2245, 2700, 2867, 2884, 2904
RsaNI GTAC 9 cut(s) 9, 66, 980, 1265, 2244, 2699, 2866, 2883, 2903
RseI CAYNNNNRTG 3 cut(s) 721, 1107, 2270
SatI GCNGC 6 cut(s) 612, 1725, 1732, 1941, 2888, 2891
Sau96I GGNCC 9 cut(s) 36, 37, 1072, 1495, 2563, 2618, 2753, 2754, 2836
ScaI AGTACT 2 cut(s) 67, 2867
SchI GAGTC 4 cut(s) 545, 926, 1792, 3024
ScrFI CCNGG 5 cut(s) 173, 442, 972, 1137, 2001
SduI GDGCHC 5 cut(s) 40, 254, 1575, 2504, 2757
SfaNI GCATC 8 cut(s) 316, 902, 1282, 1864, 1901, 2593, 3000, 3129
SfcI CTRYAG 3 cut(s) 1822, 1917, 2551
SfoI GGCGCC 1 cut(s) 100
SfuI TTCGAA 1 cut(s) 360
SinI GGWCC 4 cut(s) 1072, 1495, 2563, 2618
SmiMI CAYNNNNRTG 3 cut(s) 721, 1107, 2270
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
SphI GCATGC 2 cut(s) 309, 3120
SsiI CCGC 9 cut(s) 34, 57, 95, 109, 202, 1407, 1819, 2072, 3099
SspDI GGCGCC 1 cut(s) 98
SspI AATATT 1 cut(s) 2495
SspMI CTAG 9 cut(s) 285, 555, 909, 1016, 2055, 2241, 2292, 2741, 2918
StyD4I CCNGG 5 cut(s) 171, 440, 970, 1135, 1999
StyI CCWWGG 3 cut(s) 888, 1022, 1498
TaiI ACGT 6 cut(s) 145, 439, 598, 1887, 2217, 3074
TaqII GACCGA 2 cut(s) 638, 1764
TatI WGTACW 5 cut(s) 8, 65, 979, 2243, 2865
TfiI GAWTC 9 cut(s) 404, 946, 1670, 1901, 1967, 2170, 2407, 2872, 3046
TscAI CASTG 4 cut(s) 25, 1452, 1684, 2560
TseFI GTSAC 4 cut(s) 16, 1109, 1225, 1449
TseI GCWGC 6 cut(s) 611, 1724, 1731, 1940, 2887, 2890
Tsp45I GTSAC 4 cut(s) 16, 1109, 1225, 1449
TspGWI ACGGA 1 cut(s) 73
TspRI CASTG 4 cut(s) 25, 1452, 1684, 2560
VneI GTGCAC 1 cut(s) 2500
VpaK11BI GGWCC 4 cut(s) 1072, 1495, 2563, 2618
XagI CCTNNNNNAGG 2 cut(s) 1315, 2627
XapI RAATTY 9 cut(s) 116, 491, 739, 1523, 1664, 1840, 1992, 2467, 2645
XbaI TCTAGA 1 cut(s) 2740
XceI RCATGY 8 cut(s) 128, 309, 478, 1067, 1772, 2299, 2359, 3120
XmiI GTMKAC 1 cut(s) 2728
XspI CTAG 9 cut(s) 285, 555, 909, 1016, 2055, 2241, 2292, 2741, 2918
ZrmI AGTACT 2 cut(s) 67, 2867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.