MD00G1097600.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003891
Physical Location & Seq
Reverse (-)
20412328 .. 20413454
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1097600.v1.1.491

Sequence Viewer

Length: 564 bp
ATGGAACAGTTGCAATTAACTGCAGAATCTCAGCAGGAGCATGTTGAGGACCACCACCTCTATTCGCTAAATTATCTGCACTGGGGTGACCCAAAAGTATGGTATGGAGTCTCTGGAAGCCATGCTACGGATTTGGAACGTACAATGAGAGCGTATTTGCCTGATCTGTTCGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAGCTCTCTCCTACAGTTTTAAAGTCTGAAGGAGTACCTGTGCATCGAGCCGTCCAGCATTCTGGGGAGTTTGTTCTTACCTTCCCGCGGGCATGCCATTCTGGATTTAATTGTGGCTTCAACTGTGCTGAGGCAGTGAACGTGGCCCCTGTTGATTGGTTAGAACATGGGCAAAATGCAGTTGAGCTCTACAGTGAGCAGTGTCGCAAGACAGCAATTTCACATGACAAATTGCTACTGGGATCAGCTCGGGAAGCTGTACTTCTGGGAAAAATCAGTTATTGGAAAGAAAACTACAAGAAATATGAGCTGGCAAAGTGTTTGTGGCAAAGATGGTCTACTCACCAGAGCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

188

Amino Acids

21.42

Weight (kDa)

5.73

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 13 - 117 7.1e-36 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 545
AccII CGCG 1 cut(s) 295
AciI CCGC 2 cut(s) 293, 295
AclWI GGATC 1 cut(s) 457
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 3 cut(s) 142, 243, 468
AfiI CCNNNNNNNGG 2 cut(s) 127, 294
AgsI TTSAA 1 cut(s) 328
AleI CACNNNNGTG 1 cut(s) 84
AluBI AGCT 5 cut(s) 211, 394, 455, 464, 517
AluI AGCT 5 cut(s) 211, 394, 455, 464, 517
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 1 cut(s) 457
Ama87I CYCGRG 1 cut(s) 456
AoxI GGCC 1 cut(s) 351
AspS9I GGNCC 2 cut(s) 49, 352
AsuHPI GGTGA 2 cut(s) 98, 542
AvaI CYCGRG 1 cut(s) 456
AvaII GGWCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 396
BarI GAAGNNNNNNTAC 2 cut(s) 109, 141
Bbv12I GWGCWC 1 cut(s) 396
BbvCI CCTCAGC 1 cut(s) 336
BccI CCATC 1 cut(s) 534
BceAI ACGGC 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 115
BfmI CTRYAG 3 cut(s) 21, 219, 397
Bme18I GGWCC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 456
BmgT120I GGNCC 2 cut(s) 49, 352
BmiI GGNNCC 1 cut(s) 354
BmrI ACTGGG 2 cut(s) 91, 455
BmsI GCATC 1 cut(s) 259
BmuI ACTGGG 2 cut(s) 91, 455
Bpu10I CCTNAGC 1 cut(s) 336
BsaJI CCNNGG 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 231, 261
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 294
Bse1I ACTGG 3 cut(s) 86, 204, 450
BseDI CCNNGG 1 cut(s) 293
BseLI CCNNNNNNNGG 2 cut(s) 127, 294
BseMII CTCAG 3 cut(s) 44, 221, 327
BseNI ACTGG 3 cut(s) 86, 204, 450
BsgI GTGCAG 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 295
BshFI GGCC 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 456
BslI CCNNNNNNNGG 2 cut(s) 127, 294
BsmAI GTCTC 1 cut(s) 115
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 1 cut(s) 353
BsoBI CYCGRG 1 cut(s) 456
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 2 cut(s) 163, 449
BspACI CCGC 2 cut(s) 293, 295
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 3 cut(s) 43, 220, 328
BspFNI CGCG 1 cut(s) 295
BspLI GGNNCC 1 cut(s) 354
BspMAI CTGCAG 1 cut(s) 25
BspPI GGATC 1 cut(s) 457
BsrI ACTGG 3 cut(s) 86, 204, 450
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 2 cut(s) 163, 449
Bst4CI ACNGT 4 cut(s) 9, 223, 332, 401
BstC8I GCNNGC 3 cut(s) 297, 301, 519
BstDEI CTNAG 3 cut(s) 30, 207, 336
BstDSI CCRYGG 1 cut(s) 293
BstEII GGTNACC 1 cut(s) 86
BstFNI CGCG 1 cut(s) 295
BstKTI GATC 2 cut(s) 166, 452
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 2 cut(s) 163, 449
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstNSI RCATGY 2 cut(s) 44, 303
BstPI GGTNACC 1 cut(s) 86
BstSFI CTRYAG 3 cut(s) 21, 219, 397
BstUI CGCG 1 cut(s) 295
BstXI CCANNNNNNTGG 2 cut(s) 99, 269
BsuRI GGCC 1 cut(s) 353
BtgI CCRYGG 1 cut(s) 293
BtsI GCAGTG 2 cut(s) 348, 413
BtsIMutI CAGTG 4 cut(s) 79, 348, 406, 413
Cac8I GCNNGC 3 cut(s) 297, 301, 519
Cfr13I GGNCC 2 cut(s) 49, 352
Cfr42I CCGCGG 1 cut(s) 296
Csp6I GTAC 3 cut(s) 141, 242, 467
CviAII CATG 5 cut(s) 41, 122, 300, 374, 431
CviJI RGCY 9 cut(s) 120, 211, 257, 324, 353, 394, 455, 464, 517
CviKI_1 RGCY 9 cut(s) 120, 211, 257, 324, 353, 394, 455, 464, 517
CviQI GTAC 3 cut(s) 141, 242, 467
DdeI CTNAG 3 cut(s) 30, 207, 336
DpnI GATC 2 cut(s) 165, 451
DpnII GATC 2 cut(s) 163, 449
DraI TTTAAA 1 cut(s) 228
Ecl136II GAGCTC 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 456
Eco91I GGTNACC 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 394
EcoO65I GGTNACC 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 396
FaeI CATG 5 cut(s) 44, 125, 303, 377, 434
FaiI YATR 8 cut(s) 42, 100, 105, 123, 301, 375, 432, 513
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 5 cut(s) 40, 121, 299, 373, 430
FauI CCCGC 2 cut(s) 288, 300
FblI GTMKAC 1 cut(s) 545
FriOI GRGCYC 1 cut(s) 396
HaeIII GGCC 1 cut(s) 353
Hin1II CATG 5 cut(s) 44, 125, 303, 377, 434
HinfI GANTC 2 cut(s) 26, 108
HphI GGTGA 2 cut(s) 98, 542
Hpy166II GTNNAC 2 cut(s) 346, 546
Hpy188I TCNGA 1 cut(s) 235
Hpy188III TCNNGA 3 cut(s) 114, 309, 458
Hpy8I GTNNAC 2 cut(s) 346, 546
HpyAV CCTTC 2 cut(s) 230, 298
HpyCH4III ACNGT 4 cut(s) 9, 223, 332, 401
HpyCH4IV ACGT 2 cut(s) 139, 348
HpyCH4V TGCA 5 cut(s) 13, 23, 79, 250, 386
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 3 cut(s) 30, 207, 336
HpySE526I ACGT 2 cut(s) 139, 348
Hsp92II CATG 5 cut(s) 44, 125, 303, 377, 434
KspI CCGCGG 1 cut(s) 296
Kzo9I GATC 2 cut(s) 163, 449
LmnI GCTCC 1 cut(s) 37
LweI GCATC 1 cut(s) 259
MaeII ACGT 2 cut(s) 139, 348
MaeIII GTNAC 2 cut(s) 86, 202
MalI GATC 2 cut(s) 165, 451
MboI GATC 2 cut(s) 163, 449
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 5 cut(s) 14, 70, 316, 423, 437
MlyI GAGTC 1 cut(s) 117
MnlI CCTC 4 cut(s) 40, 68, 166, 331
MseI TTAA 4 cut(s) 17, 227, 315, 562
MslI CAYNNNNRTG 1 cut(s) 84
MspA1I CMGCKG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 265
MvnI CGCG 1 cut(s) 295
MwoI GCNNNNNNNGC 1 cut(s) 461
NdeII GATC 2 cut(s) 163, 449
NlaIII CATG 5 cut(s) 44, 125, 303, 377, 434
NlaIV GGNNCC 1 cut(s) 354
NmuCI GTSAC 2 cut(s) 86, 202
NspI RCATGY 2 cut(s) 44, 303
OliI CACNNNNGTG 1 cut(s) 84
PaeI GCATGC 1 cut(s) 303
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 1 cut(s) 26
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 396
PspEI GGTNACC 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 2 cut(s) 49, 352
PstI CTGCAG 1 cut(s) 25
RsaI GTAC 3 cut(s) 142, 243, 468
RsaNI GTAC 3 cut(s) 141, 242, 467
RseI CAYNNNNRTG 1 cut(s) 84
SacI GAGCTC 1 cut(s) 396
SacII CCGCGG 1 cut(s) 296
SaqAI TTAA 4 cut(s) 17, 227, 315, 562
Sau3AI GATC 2 cut(s) 163, 449
Sau96I GGNCC 2 cut(s) 49, 352
SchI GAGTC 1 cut(s) 117
SduI GDGCHC 1 cut(s) 396
SfaNI GCATC 1 cut(s) 259
SfcI CTRYAG 3 cut(s) 21, 219, 397
Sfr303I CCGCGG 1 cut(s) 296
SgrBI CCGCGG 1 cut(s) 296
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 84
SphI GCATGC 1 cut(s) 303
Sse9I AATT 5 cut(s) 14, 70, 316, 423, 437
SsiI CCGC 2 cut(s) 293, 295
SstI GAGCTC 1 cut(s) 396
TaaI ACNGT 4 cut(s) 9, 223, 332, 401
TaiI ACGT 2 cut(s) 142, 351
TaqI TCGA 2 cut(s) 171, 253
TasI AATT 5 cut(s) 14, 70, 316, 423, 437
TatI WGTACW 1 cut(s) 466
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 4 cut(s) 17, 227, 315, 562
Tru9I TTAA 4 cut(s) 17, 227, 315, 562
TscAI CASTG 4 cut(s) 86, 348, 406, 413
TseFI GTSAC 2 cut(s) 86, 202
Tsp45I GTSAC 2 cut(s) 86, 202
TspDTI ATGAA 1 cut(s) 210
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 4 cut(s) 86, 348, 406, 413
VpaK11BI GGWCC 1 cut(s) 49
XceI RCATGY 2 cut(s) 44, 303
XmiI GTMKAC 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.