MD10G1241100.v1.1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003891
Physical Location & Seq
Reverse (-)
33658731 .. 33664694
5964 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1241100.v1.1.491

Sequence Viewer

Length: 3123 bp
ATGGAACAGTTACAATTAACTGTCGAATCTCAGCAGAAGGAGGATCATTCTTCTAAGAACTACCACAAAAGTGATAGCACACTTGAGGGCTCTGGCAGCCCTCGAATCCGAAAGATATCAGCTAGATGGAACCCAGAGGAAGCATGCAGACCTGATATTGATGAAGCGCCTGTGTTTTATCCCACTATTGAGGAGTTTGAAGATACACTTGGTTACATAGCAAAGATCCGCCCACTGGCCGAATCTTATGGTATATGCCGGATTGTCCCTCCACCTTCGTGGACCCCTCCCTGCCCTCTTAAGGAGAAAGAGATGTGGGAACATGCCAAATTTTCTACGCGCATTCAGCAAGTTGACTTGCTTCAAAACAGAGAGGCCATGAGAAAGAAAAGTAGAAGTCGGAAGCGTAAACGAAAGAGGAACTCAAGAATGGGAGCCAGAAGACGTTCAGAAGCTACTGCTGAGGCTGATGAGAAGTTTGGGTTCCATTCAGGATCAGACTTTACATTTGAAGAGTTTCAGAAACATGCTGCTACTTTTAAGGAGAGTTACTTTGGAACAAAGGATGCTAAGGAGGGTTCAACTTATGGTGAAACTAAAAGCGAAACATGGCAACCCTCTGTGGAGGATATCGAAGGTGAATACTGGCGAATAGTTGAGACGCCAACAACAGATGAGGTTGAGGTTTACTATGGAGCTGACTTGGAAACAGGAGTATTTGGAAGTGGTTTTCCTAAGGCATCATCAACAGGAAGTGGTTCGGATAAGTATGCATTGTCAGGTTGGAATCTAAATAACTTCCCACGCCTGCCCGGTTCTGTACTGTGTTTTGAAGCAAGCGATATCTCAGGAGTTCTAGTCCCATGGCTCTATGTTGGGATGTGCTTTTCTTCATTTTGTTGGCATGTTGAGGACCACCACCTCTATTCGCTAAATTATCTGCACTGGGGTGACCCAAAAGTATGGTATGGAGTCTCTGGAAGCCATGCTACTGATTTGGAACGTACAATGAGAGCGTATTTACCTGATCTATTCGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAACTATCTCCTACAGTTTTAAAGTCTGAAGGTGTACCTGTGCATCGAGCTGTCCAGCATTCTGGGGAGTTTGTTCTTACCTTCCCGCGGGCATACCATTCTGGATTTAATTGTGGTTTTAACTGTGCTGAGGCAGTGAACGTGGCCCCTGTTGATTGGTTAGAACATGGGCAAAATGCAGTTGAGCTCTACAGTGAGCAGTGTCGCAAGACATCAATCTCACACGACAAATTGTTACTGGGATCAGCTCGGGAGGCTGTACAGGCCCTCTGGGAACAATCAGTTCTTGGAAAGAAAACTACAAGAAATATGAGCTGGCAAATTGTTTGTGGCAAAGATGGTCTACTCACCAGAGCAATTAAGACACGGGTGCGAATGGAGGAGGAAAGACTAGATCGTCTACCAATTTGTATGAAGTTGAAAAAGATGGAAATAGACTTTGATTTGAACAATGAGAGAGAATGTTTTTCTTGCTTCTATGACTTGCACTTATCTGCCTCCAGCTGCAAATGCTCTCCTGACCGATTTTCATGTCTCAAACATGCAAAGCTCCTTTGTTCATGTCACATCAATCAAAGATATGTCCTTCAACGCCACACCATCAATGAATTAAATATGCTGGTTGAAGCATTGGAAGGGAAAGTTGAGGCCATAAAAGTATGGGCATCCGAAGACCATGAATTAGATGGTACTGATACACGTACAGCTAAGCTGGATGAAGAAAGTGGCATGCCTCGTAAAAGAATCAAATCTTGTGATCCAAGGGAAACTTCACCTTGCTGCCCAGTTTCAGAAGAGAAGGTGAACATAAATGCATCCAGCTGTTCGTCCAGTCAGGTTTCTTCTGCAGTTGTCCAGTCAGGGTCACAGCATGGAGCTTCTAGTTTAAGTACGTCTCCCATTGCTATGGATAGTCAAAATGACATTCAAATTCTGGTAAAGAATGATGAAGCAAAGACGGGGATGGAGTGCTTTGATCTGAATCTTAATTACATGTCTGAAGAACATGAAAGCAGGACAATGCACACATCTGATCATTGTGATAACAAGGCTGTCACAATTGAGGAGGAGACCTCTACGTCAGCGTCTAATCAAGAGAAAGTTTGTAGTTCAGATGTAGCCAGAGAACCAGACATGATGAAAGTTGATGATGACTGTAATGTATCTGCTCTGACAGTTCTGAACAATGACTACCCTGCCGGTTCAAGGGACATCAGGAATAACTGTGCGAGTGAGGGCAATAAGTTGTTTGGTGTAAATATATATGTGACTGACCAAAGCTATCAATTACAAGAGCTGAGTCCTTCTGTTGAGCCTATAGATTTTGGAGCTGTTGTCTCTGGGAAGTTGTGGTGCAGTAAGCAGGCAATATATCCAAAAGGTTATAGAAGCCGAGTTAGGTTTTATAGTGTGCTTGACCCAACAAAAGTATGTAGCTATATTTCAGAAGTTGTCGATGCTGGGCTTCTTGGTCCTCTATTCAAGGTTAGTGTGGAAGATTTCCCAGGTGAGGTTTTCGCAAATGTATCGGCAGACAAGTGCTGGGAAATGGTACTACAGAGACTACATCAAGAAATAAACAGACGGAGTAGTGTAGGGGAAAACGGACTGCCCCATATGCAGTCATTGCGGAGCATTAATGGGCTCGAAATGTTTGGATTTCTCTCCCAACCCATTGCTGAGGCTATTGAGGCTCATGATCCTGACCATCAATGCGTGGAGTACTGGAATCACAGGCACATGGTCCCGTCAATGTCTTGCATGCTTAGTGAAATCAAGCAGCATTCATTCAAACAAAGTTGCTGTCTACGAGAAATGGAGACAAAAGTTTTTGGTGTCAGTTTGACAAAGCCAGATCAGGATTGTTCATCGGTGGAACATCATCATTTAACCGAAGAGATGCAGTTGCTACTACGAAGACTACTAAAGAAGGCGGATCCTGAGGAGTTAAGTGCATTACAGAGAGTATTCTGCAGCGAGTCACAAAGTGCGAAATGGAGAGTGGCATTCGCATCAATGATCGAAGAGATCCAGAAACATGTAGATAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1041

Amino Acids

117.64

Weight (kDa)

5.53

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 57 - 90 6.2e-17 jmjN domain
JmjC PF02373 289 - 405 4.2e-44 JmjC domain, hydroxylase
zf-C5HC2 PF02928 512 - 562 2.3e-12 C5HC2 zinc finger
FYRN PF05964 797 - 843 8.5e-12 F/Y-rich N-terminus
FYRC PF05965 848 - 936 1.1e-19 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1467, 2149
AccI GTMKAC 3 cut(s) 1414, 1471, 2878
AccII CGCG 2 cut(s) 340, 1159
AciI CCGC 5 cut(s) 229, 1157, 1159, 2701, 3005
AclWI GGATC 9 cut(s) 51, 220, 502, 1321, 1823, 2765, 3002, 3015, 3094
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 2 cut(s) 329, 2001
AcuI CTGAAG 2 cut(s) 1119, 2091
AcyI GRCGYC 1 cut(s) 662
AdeI CACNNNGTG 1 cut(s) 3059
AfaI GTAC 9 cut(s) 822, 1006, 1107, 1332, 1762, 1774, 1963, 2625, 2795
AfiI CCNNNNNNNGG 5 cut(s) 235, 301, 1158, 2277, 2581
AflII CTTAAG 1 cut(s) 299
AflIII ACRYGT 3 cut(s) 1769, 2064, 3109
AjnI CCWGG 1 cut(s) 2575
AjuI GAANNNNNNNTTGG 4 cut(s) 192, 224, 1825, 1857
AleI CACNNNNGTG 3 cut(s) 69, 277, 948
Alw21I GWGCWC 1 cut(s) 1260
Alw26I GTCTC 8 cut(s) 653, 979, 1610, 1971, 2135, 2413, 2626, 2885
AlwI GGATC 9 cut(s) 51, 220, 502, 1321, 1823, 2765, 3002, 3015, 3094
Ama87I CYCGRG 1 cut(s) 1320
AoxI GGCC 5 cut(s) 237, 375, 1215, 1335, 1719
ApeKI GCWGC 6 cut(s) 96, 530, 1575, 1851, 2851, 3045
ApoI RAATTY 2 cut(s) 329, 2001
ArsI GACNNNNNNTTYG 4 cut(s) 491, 523, 2860, 2892
AseI ATTAAT 1 cut(s) 2709
Asp700I GAANNNNTTC 3 cut(s) 445, 516, 757
AspLEI GCGC 2 cut(s) 169, 342
AspS9I GGNCC 6 cut(s) 282, 913, 1216, 1336, 2543, 2815
AsuC2I CCSGG 1 cut(s) 813
AsuHPI GGTGA 7 cut(s) 602, 650, 962, 1411, 1836, 1885, 2591
AvaI CYCGRG 1 cut(s) 1320
AvaII GGWCC 4 cut(s) 282, 913, 2543, 2815
AxyI CCTNAGG 2 cut(s) 735, 3012
BamHI GGATCC 1 cut(s) 3007
BanII GRGCYC 3 cut(s) 92, 1260, 2718
BarI GAAGNNNNNNTAC 2 cut(s) 973, 1005
BbsI GAAGAC 3 cut(s) 448, 1749, 2995
Bbv12I GWGCWC 1 cut(s) 1260
BbvCI CCTCAGC 3 cut(s) 462, 1200, 2751
BbvI GCAGC 6 cut(s) 108, 517, 1562, 1838, 2863, 3057
BccI CCATC 7 cut(s) 120, 1403, 1492, 1679, 1751, 2029, 2787
BcgI CGANNNNNNTGC 2 cut(s) 2580, 2614
BciT130I CCWGG 1 cut(s) 2577
BclI TGATCA 1 cut(s) 2104
BcnI CCSGG 1 cut(s) 813
BcoDI GTCTC 8 cut(s) 653, 979, 1610, 1971, 2135, 2413, 2626, 2885
BfaI CTAG 4 cut(s) 123, 857, 1463, 1953
BfmI CTRYAG 6 cut(s) 1083, 1261, 1917, 2388, 2627, 3043
BfoI RGCGCY 1 cut(s) 170
BfrI CTTAAG 1 cut(s) 299
BisI GCNGC 6 cut(s) 97, 531, 1576, 1852, 2852, 3046
BlpI GCTNAGC 1 cut(s) 1779
BlsI GCNGC 6 cut(s) 98, 532, 1577, 1853, 2853, 3047
BmcAI AGTACT 1 cut(s) 2795
Bme1390I CCNGG 2 cut(s) 813, 2577
Bme18I GGWCC 4 cut(s) 282, 913, 2543, 2815
BmeT110I CYCGRG 1 cut(s) 1320
BmgT120I GGNCC 6 cut(s) 282, 913, 1216, 1336, 2543, 2815
BmiI GGNNCC 7 cut(s) 131, 284, 436, 485, 1218, 2817, 3009
BmrFI CCNGG 2 cut(s) 813, 2577
BmrI ACTGGG 3 cut(s) 955, 1319, 1850
BmsI GCATC 8 cut(s) 556, 749, 1123, 1745, 1895, 2518, 2961, 3092
BmuI ACTGGG 3 cut(s) 955, 1319, 1850
BpiI GAAGAC 3 cut(s) 448, 1749, 2995
BplI GAGNNNNNCTC 2 cut(s) 2129, 2161
BpmI CTGGAG 1 cut(s) 1555
Bpu10I CCTNAGC 4 cut(s) 462, 570, 1200, 2751
Bpu1102I GCTNAGC 1 cut(s) 1779
BpuEI CTTGAG 2 cut(s) 104, 409
BpuMI CCSGG 1 cut(s) 813
BsaAI YACGTR 1 cut(s) 1772
BsaBI GATNNNNATC 1 cut(s) 2052
BsaHI GRCGYC 1 cut(s) 662
BsaI GGTCTC 1 cut(s) 2135
BsaJI CCNNGG 4 cut(s) 863, 1157, 1832, 2575
BsaXI ACNNNNNCTCC 4 cut(s) 843, 873, 2391, 2421
Bsc4I CCNNNNNNNGG 5 cut(s) 235, 301, 1158, 2277, 2581
Bse118I RCCGGY 1 cut(s) 2270
Bse1I ACTGG 9 cut(s) 240, 650, 950, 1068, 1314, 1856, 1902, 1927, 2801
Bse21I CCTNAGG 2 cut(s) 735, 3012
Bse3DI GCAATG 3 cut(s) 1971, 2696, 2745
Bse8I GATNNNNATC 1 cut(s) 2052
BseBI CCWGG 1 cut(s) 2577
BseDI CCNNGG 4 cut(s) 863, 1157, 1832, 2575
BseGI GGATG 6 cut(s) 571, 885, 1736, 1792, 1886, 2040
BseJI GATNNNNATC 1 cut(s) 2052
BseLI CCNNNNNNNGG 5 cut(s) 235, 301, 1158, 2277, 2581
BseMI GCAATG 3 cut(s) 1971, 2696, 2745
BseMII CTCAG 7 cut(s) 44, 453, 861, 1191, 2360, 2742, 3003
BseNI ACTGG 9 cut(s) 240, 650, 950, 1068, 1314, 1856, 1902, 1927, 2801
BseRI GAGGAG 5 cut(s) 206, 1466, 2150, 2153, 3029
BseXI GCAGC 6 cut(s) 108, 517, 1562, 1838, 2863, 3057
BseYI CCCAGC 2 cut(s) 2531, 2613
BsgI GTGCAG 2 cut(s) 926, 2446
Bsh1236I CGCG 2 cut(s) 340, 1159
BshFI GGCC 5 cut(s) 239, 377, 1217, 1337, 1721
BsiHKAI GWGCWC 1 cut(s) 1260
BsiHKCI CYCGRG 1 cut(s) 1320
BsiSI CCGG 3 cut(s) 259, 813, 2271
BslFI GGGAC 4 cut(s) 251, 845, 2294, 2801
BslI CCNNNNNNNGG 5 cut(s) 235, 301, 1158, 2277, 2581
BsmAI GTCTC 8 cut(s) 653, 979, 1610, 1971, 2135, 2413, 2626, 2885
BsmBI CGTCTC 2 cut(s) 653, 1971
BsmFI GGGAC 4 cut(s) 251, 845, 2294, 2801
BsmI GAATGC 4 cut(s) 342, 1129, 2854, 3077
BsnI GGCC 5 cut(s) 239, 377, 1217, 1337, 1721
Bso31I GGTCTC 1 cut(s) 2135
BsoBI CYCGRG 1 cut(s) 1320
Bsp1286I GDGCHC 3 cut(s) 92, 1260, 2718
Bsp1407I TGTACA 1 cut(s) 1330
Bsp1720I GCTNAGC 1 cut(s) 1779
Bsp19I CCATGG 1 cut(s) 863
BspACI CCGC 5 cut(s) 229, 1157, 1159, 2701, 3005
BspANI GGCC 5 cut(s) 239, 377, 1217, 1337, 1721
BspCNI CTCAG 7 cut(s) 43, 454, 860, 1192, 2361, 2743, 3004
BspFNI CGCG 2 cut(s) 340, 1159
BspHI TCATGA 1 cut(s) 2767
BspLI GGNNCC 7 cut(s) 131, 284, 436, 485, 1218, 2817, 3009
BspMAI CTGCAG 2 cut(s) 1921, 3047
BspPI GGATC 9 cut(s) 51, 220, 502, 1321, 1823, 2765, 3002, 3015, 3094
BspTI CTTAAG 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 2135
BsrDI GCAATG 3 cut(s) 1971, 2696, 2745
BsrFI RCCGGY 1 cut(s) 2270
BsrGI TGTACA 1 cut(s) 1330
BsrI ACTGG 9 cut(s) 240, 650, 950, 1068, 1314, 1856, 1902, 1927, 2801
BssAI RCCGGY 1 cut(s) 2270
BssECI CCNNGG 4 cut(s) 863, 1157, 1832, 2575
BssNI GRCGYC 1 cut(s) 662
BssT1I CCWWGG 2 cut(s) 863, 1832
Bst2UI CCWGG 1 cut(s) 2577
Bst4CI ACNGT 9 cut(s) 9, 22, 825, 1087, 1196, 1265, 2228, 2248, 2297
Bst6I CTCTTC 4 cut(s) 507, 1860, 2961, 3090
BstACI GRCGYC 1 cut(s) 662
BstAFI CTTAAG 1 cut(s) 299
BstAPI GCANNNNNTGC 1 cut(s) 2698
BstAUI TGTACA 1 cut(s) 1330
BstBAI YACGTR 1 cut(s) 1772
BstC8I GCNNGC 8 cut(s) 145, 809, 838, 1161, 1388, 1802, 2436, 2834
BstDSI CCRYGG 2 cut(s) 863, 1157
BstEII GGTNACC 1 cut(s) 950
BstF5I GGATG 6 cut(s) 571, 885, 1736, 1792, 1886, 2040
BstFNI CGCG 2 cut(s) 340, 1159
BstH2I RGCGCY 1 cut(s) 170
BstHHI GCGC 2 cut(s) 169, 342
BstMAI GTCTC 8 cut(s) 653, 979, 1610, 1971, 2135, 2413, 2626, 2885
BstMWI GCNNNNNNNGC 8 cut(s) 96, 346, 1325, 1334, 1581, 2689, 2698, 2762
BstNI CCWGG 1 cut(s) 2577
BstNSI RCATGY 9 cut(s) 147, 326, 530, 908, 1616, 1804, 2068, 2836, 3113
BstPI GGTNACC 1 cut(s) 950
BstSCI CCNGG 2 cut(s) 811, 2575
BstSFI CTRYAG 6 cut(s) 1083, 1261, 1917, 2388, 2627, 3043
BstUI CGCG 2 cut(s) 340, 1159
BstV1I GCAGC 6 cut(s) 108, 517, 1562, 1838, 2863, 3057
BstV2I GAAGAC 3 cut(s) 448, 1749, 2995
BstX2I RGATCY 3 cut(s) 225, 3007, 3099
BstXI CCANNNNNNTGG 4 cut(s) 279, 963, 1133, 1978
BstYI RGATCY 3 cut(s) 225, 3007, 3099
Bsu36I CCTNAGG 2 cut(s) 735, 3012
BsuRI GGCC 5 cut(s) 239, 377, 1217, 1337, 1721
BtgI CCRYGG 2 cut(s) 863, 1157
BtsCI GGATG 6 cut(s) 571, 885, 1736, 1792, 1886, 2040
BtsI GCAGTG 2 cut(s) 1212, 1277
BtsIMutI CAGTG 5 cut(s) 233, 943, 1212, 1270, 1277
Cac8I GCNNGC 8 cut(s) 145, 809, 838, 1161, 1388, 1802, 2436, 2834
CciI TCATGA 1 cut(s) 2767
CfoI GCGC 2 cut(s) 169, 342
Cfr10I RCCGGY 1 cut(s) 2270
Cfr13I GGNCC 6 cut(s) 282, 913, 1216, 1336, 2543, 2815
Cfr42I CCGCGG 1 cut(s) 1160
CseI GACGC 2 cut(s) 670, 2145
Csp6I GTAC 9 cut(s) 821, 1005, 1106, 1331, 1761, 1773, 1962, 2624, 2794
CviQI GTAC 9 cut(s) 821, 1005, 1106, 1331, 1761, 1773, 1962, 2624, 2794
DraI TTTAAA 1 cut(s) 1092
DraIII CACNNNGTG 1 cut(s) 3059
DrdI GACNNNNNNGTC 2 cut(s) 1467, 2149
DseDI GACNNNNNNGTC 2 cut(s) 1467, 2149
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 4 cut(s) 507, 1860, 2961, 3090
EarI CTCTTC 4 cut(s) 507, 1860, 2961, 3090
EciI GGCGGA 2 cut(s) 218, 3020
Ecl136II GAGCTC 1 cut(s) 1258
Eco130I CCWWGG 2 cut(s) 863, 1832
Eco24I GRGCYC 3 cut(s) 92, 1260, 2718
Eco31I GGTCTC 1 cut(s) 2135
Eco32I GATATC 3 cut(s) 117, 631, 844
Eco47I GGWCC 4 cut(s) 282, 913, 2543, 2815
Eco53kI GAGCTC 1 cut(s) 1258
Eco57I CTGAAG 2 cut(s) 1119, 2091
Eco81I CCTNAGG 2 cut(s) 735, 3012
Eco88I CYCGRG 1 cut(s) 1320
Eco91I GGTNACC 1 cut(s) 950
EcoICRI GAGCTC 1 cut(s) 1258
EcoO109I RGGNCCY 1 cut(s) 1336
EcoO65I GGTNACC 1 cut(s) 950
EcoRII CCWGG 1 cut(s) 2575
EcoRV GATATC 3 cut(s) 117, 631, 844
EcoT14I CCWWGG 2 cut(s) 863, 1832
EcoT22I ATGCAT 2 cut(s) 775, 1888
EcoT38I GRGCYC 3 cut(s) 92, 1260, 2718
ErhI CCWWGG 2 cut(s) 863, 1832
Esp3I CGTCTC 2 cut(s) 653, 1971
FalI AAGNNNNNCTT 4 cut(s) 192, 224, 1825, 1857
FaqI GGGAC 4 cut(s) 251, 845, 2294, 2801
FauI CCCGC 2 cut(s) 1152, 1164
FauNDI CATATG 1 cut(s) 2688
FbaI TGATCA 1 cut(s) 2104
FblI GTMKAC 3 cut(s) 1414, 1471, 2878
Fnu4HI GCNGC 6 cut(s) 97, 531, 1576, 1852, 2852, 3046
FokI GGATG 6 cut(s) 578, 892, 1723, 1799, 1873, 2047
FriOI GRGCYC 3 cut(s) 92, 1260, 2718
Fsp4HI GCNGC 6 cut(s) 97, 531, 1576, 1852, 2852, 3046
FspBI CTAG 4 cut(s) 123, 857, 1463, 1953
GlaI GCGC 2 cut(s) 168, 341
GluI GCNGC 6 cut(s) 97, 531, 1576, 1852, 2852, 3046
GsaI CCCAGC 2 cut(s) 2535, 2617
GsuI CTGGAG 1 cut(s) 1555
HaeII RGCGCY 1 cut(s) 170
HaeIII GGCC 5 cut(s) 239, 377, 1217, 1337, 1721
HapII CCGG 3 cut(s) 259, 813, 2271
HgaI GACGC 2 cut(s) 670, 2145
HhaI GCGC 2 cut(s) 169, 342
Hin1I GRCGYC 1 cut(s) 662
Hin6I GCGC 2 cut(s) 167, 340
HinP1I GCGC 2 cut(s) 167, 340
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HpaII CCGG 3 cut(s) 259, 813, 2271
HphI GGTGA 7 cut(s) 602, 650, 962, 1411, 1836, 1885, 2591
HpyCH4III ACNGT 9 cut(s) 9, 22, 825, 1087, 1196, 1265, 2228, 2248, 2297
HpyCH4IV ACGT 6 cut(s) 445, 1003, 1212, 1771, 1964, 2150
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 346, 1325, 1334, 1581, 2689, 2698, 2762
HpySE526I ACGT 6 cut(s) 445, 1003, 1212, 1771, 1964, 2150
Hsp92I GRCGYC 1 cut(s) 662
HspAI GCGC 2 cut(s) 167, 340
Ksp22I TGATCA 1 cut(s) 2104
KspI CCGCGG 1 cut(s) 1160
LmnI GCTCC 6 cut(s) 434, 695, 1626, 1946, 2399, 2703
Lsp1109I GCAGC 6 cut(s) 108, 517, 1562, 1838, 2863, 3057
LweI GCATC 8 cut(s) 556, 749, 1123, 1745, 1895, 2518, 2961, 3092
MaeI CTAG 4 cut(s) 123, 857, 1463, 1953
MaeII ACGT 6 cut(s) 445, 1003, 1212, 1771, 1964, 2150
MfeI CAATTG 1 cut(s) 2130
MflI RGATCY 3 cut(s) 225, 3007, 3099
MhlI GDGCHC 3 cut(s) 92, 1260, 2718
MlyI GAGTC 3 cut(s) 981, 2380, 3059
MmeI TCCRAC 2 cut(s) 380, 764
Mph1103I ATGCAT 2 cut(s) 775, 1888
MroXI GAANNNNTTC 3 cut(s) 445, 516, 757
MslI CAYNNNNRTG 4 cut(s) 69, 277, 948, 1976
MspA1I CMGCKG 3 cut(s) 1159, 1575, 1893
MspCI CTTAAG 1 cut(s) 299
MspI CCGG 3 cut(s) 259, 813, 2271
MspR9I CCNGG 2 cut(s) 813, 2577
MunI CAATTG 1 cut(s) 2130
Mva1269I GAATGC 4 cut(s) 342, 1129, 2854, 3077
MvaI CCWGG 1 cut(s) 2577
MvnI CGCG 2 cut(s) 340, 1159
MwoI GCNNNNNNNGC 8 cut(s) 96, 346, 1325, 1334, 1581, 2689, 2698, 2762
NciI CCSGG 1 cut(s) 813
NcoI CCATGG 1 cut(s) 863
NdeI CATATG 1 cut(s) 2688
NlaIV GGNNCC 7 cut(s) 131, 284, 436, 485, 1218, 2817, 3009
NmeAIII GCCGAG 1 cut(s) 2489
NmuCI GTSAC 7 cut(s) 950, 1066, 1634, 1935, 2125, 2338, 3051
NsiI ATGCAT 2 cut(s) 775, 1888
NspI RCATGY 9 cut(s) 147, 326, 530, 908, 1616, 1804, 2068, 2836, 3113
OliI CACNNNNGTG 3 cut(s) 69, 277, 948
PaeI GCATGC 3 cut(s) 147, 1804, 2836
PagI TCATGA 1 cut(s) 2767
PciI ACATGT 2 cut(s) 2064, 3109
PctI GAATGC 4 cut(s) 342, 1129, 2854, 3077
PdmI GAANNNNTTC 3 cut(s) 445, 516, 757
PfeI GAWTC 7 cut(s) 26, 105, 242, 787, 1815, 2053, 2800
PkrI GCNGC 6 cut(s) 98, 532, 1577, 1853, 2853, 3047
PleI GAGTC 3 cut(s) 980, 2379, 3058
PpsI GAGTC 3 cut(s) 980, 2379, 3058
Ppu21I YACGTR 1 cut(s) 1772
PscI ACATGT 2 cut(s) 2064, 3109
PshBI ATTAAT 1 cut(s) 2709
Psp124BI GAGCTC 1 cut(s) 1260
Psp6I CCWGG 1 cut(s) 2575
PspEI GGTNACC 1 cut(s) 950
PspFI CCCAGC 2 cut(s) 2531, 2613
PspGI CCWGG 1 cut(s) 2575
PspN4I GGNNCC 7 cut(s) 131, 284, 436, 485, 1218, 2817, 3009
PspPI GGNCC 6 cut(s) 282, 913, 1216, 1336, 2543, 2815
PsrI GAACNNNNNNTAC 2 cut(s) 2246, 2278
PstI CTGCAG 2 cut(s) 1921, 3047
PsuI RGATCY 3 cut(s) 225, 3007, 3099
PvuII CAGCTG 2 cut(s) 1575, 1893
RsaI GTAC 9 cut(s) 822, 1006, 1107, 1332, 1762, 1774, 1963, 2625, 2795
RsaNI GTAC 9 cut(s) 821, 1005, 1106, 1331, 1761, 1773, 1962, 2624, 2794
RseI CAYNNNNRTG 4 cut(s) 69, 277, 948, 1976
SacI GAGCTC 1 cut(s) 1260
SacII CCGCGG 1 cut(s) 1160
SatI GCNGC 6 cut(s) 97, 531, 1576, 1852, 2852, 3046
Sau96I GGNCC 6 cut(s) 282, 913, 1216, 1336, 2543, 2815
ScaI AGTACT 1 cut(s) 2795
SchI GAGTC 3 cut(s) 981, 2380, 3059
ScrFI CCNGG 2 cut(s) 813, 2577
SduI GDGCHC 3 cut(s) 92, 1260, 2718
SfaNI GCATC 8 cut(s) 556, 749, 1123, 1745, 1895, 2518, 2961, 3092
SfcI CTRYAG 6 cut(s) 1083, 1261, 1917, 2388, 2627, 3043
Sfr303I CCGCGG 1 cut(s) 1160
SgrBI CCGCGG 1 cut(s) 1160
SinI GGWCC 4 cut(s) 282, 913, 2543, 2815
SmiMI CAYNNNNRTG 4 cut(s) 69, 277, 948, 1976
SmlI CTYRAG 3 cut(s) 83, 299, 424
SmoI CTYRAG 3 cut(s) 83, 299, 424
SphI GCATGC 3 cut(s) 147, 1804, 2836
SsiI CCGC 5 cut(s) 229, 1157, 1159, 2701, 3005
SspMI CTAG 4 cut(s) 123, 857, 1463, 1953
SstI GAGCTC 1 cut(s) 1260
StyD4I CCNGG 2 cut(s) 811, 2575
StyI CCWWGG 2 cut(s) 863, 1832
TaaI ACNGT 9 cut(s) 9, 22, 825, 1087, 1196, 1265, 2228, 2248, 2297
TaiI ACGT 6 cut(s) 448, 1006, 1215, 1774, 1967, 2153
TaqI TCGA 8 cut(s) 24, 103, 633, 1035, 1117, 2526, 2718, 3093
TaqII GACCGA 1 cut(s) 1608
TatI WGTACW 3 cut(s) 820, 1330, 2793
TfiI GAWTC 7 cut(s) 26, 105, 242, 787, 1815, 2053, 2800
TscAI CASTG 5 cut(s) 240, 950, 1212, 1270, 1277
TseFI GTSAC 7 cut(s) 950, 1066, 1634, 1935, 2125, 2338, 3051
TseI GCWGC 6 cut(s) 96, 530, 1575, 1851, 2851, 3045
Tsp45I GTSAC 7 cut(s) 950, 1066, 1634, 1935, 2125, 2338, 3051
TspGWI ACGGA 2 cut(s) 2671, 2691
TspRI CASTG 5 cut(s) 240, 950, 1212, 1270, 1277
Vha464I CTTAAG 1 cut(s) 299
VpaK11BI GGWCC 4 cut(s) 282, 913, 2543, 2815
VspI ATTAAT 1 cut(s) 2709
XapI RAATTY 2 cut(s) 329, 2001
XceI RCATGY 9 cut(s) 147, 326, 530, 908, 1616, 1804, 2068, 2836, 3113
XcmI CCANNNNNNNNNTGG 1 cut(s) 1754
XmiI GTMKAC 3 cut(s) 1414, 1471, 2878
XmnI GAANNNNTTC 3 cut(s) 445, 516, 757
XspI CTAG 4 cut(s) 123, 857, 1463, 1953
ZrmI AGTACT 1 cut(s) 2795
Zsp2I ATGCAT 2 cut(s) 775, 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.