Rorug05G0042000

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003891
Physical Location & Seq
Reverse (-)
3637701 .. 3642402
4702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0042000.1

Sequence Viewer

Length: 1908 bp
ATGTCAAGATTATTGACGTTGAGGAGTTTTAGGAGAGTTGTGAATTCTGTTGTGACGAGCCAGAAACAGAGCTACACTGAAGCGGCCAAGTATGGAACAGTTGTTAGAATTGGCCTGCGGCTTCCCCAATGCAGGTACTACAGGGAGTATACCTTTCCTAATAGAGGAGAGGCCCGATTCTCATTGTACAATACCACAAAGAGCTTTTGTCAGAGTAGTTATTCCAGGAATTTTTCTACCATCCCAGCGGCAAGGAGTGCCGTGAGACACAATGCACAAATAATATGGAAAAGGCTCTCCGAAAAGTTTTATTCCAATGGTGGTTATAGATTTTCGCCAATAGATAGGATTGCTCGAGCATTCAGCTTGGGAATAACGCGCTATGGGTTGTTGTTTCCGGGCGTTTTTGCTGTCTCAAGTGGGAAGCTAGCATGGGCACAGAGGACTTTGGCAGAGAAGGACCACTATGCACCACCGCTACCACAGACTACCTTGTACACTCGTGCAGAAAATGGACATGCTTTAGTGAGCTCATTAGTATTTTCAGTTGTAGAAGGTATAATCTTGTTGCTGAGAGCAATCTATCTAGGGATCTTGTTCTCACCAAGCATAATGATGGCTCCTTTTGCTGATTCTTGTGGACCTCAGTTTAGGAGATTGTGGCTTCGTGTTGTTCTTCGTACATTGGAAAGAGCAGGTCCTGCTTTCATCAAATGGGGTCAATGGGCAGCCACTCGTCCTGATCTCTTCCCCAGAGATGTATGCACCAAACTTTCCGAGCTTCATACCAAAGCTCCTGAACATAGCTTTGCCTACACAAAGAAGACAATTGAAAAATCATTTGGCCGCAGGCTGCCCGAAATTTTTGAAAACTTTGAAGAGACACCAGTAGCATCTGGGAGTATTGCTCAAGTGCATCGGGCTACTTTGAAATTTCGATACCCTGGTCAACGGGTGAAGCCCATAGTAGTTGCAGTTAAGGTTAGACATCCTGGTGTTGGTGAATCAATTAGGAGAGATTTTGTAATAATCAATTTAGTAGCAAAAATGTCAAAGTTCATTCCTACTTTAAAGTGGTGGAGATTGGATGAAAGTGTACAACAGTTTTCAGTTTTTATGATGTCTCAAGTTGACCTTGCAAGGGAAGCTGCCAATTTGAGCCGCTTTACTTATAATTTTCGTAGATGGAAGGACGTCTCTTTCCCAAAGCCTTTGTATCCACTTGTGCATCCTGCTGTTTTAGTGGAAACGTATGAGCAAGGGGAATGCGTCTCACATTATGTTGATGATCTTCAAGGGCATGAACGCATTAAATCTGCACTTGCTCACATTGGAACACATGCACTTCTGAAGATGCTCCTGGTGGACAACTTTATACATGCAGACATGCATCCTGGGAACATACTTGTTCGAGTGGCTCAGAACAAGCCTTCTCGGAAGCGACTGTTCAAATCAAAGCCTCATGTCATTTTCCTTGATGTAGGCATGACTGCTGAACTCTCTAGAAGTGACAGAGTGAATTTACTTGAATTTTTCAAGGCTGTTGCACGTCGTGATGGGCGCACTGCTGCTGAGTGCACACTAAGACTCTCTAAGCAACAGAATTGCCCAAATCCAAAAGCTTTCATTGAGGAAGTGGAGGAGTCATTCACTTTCTGGGGTACTCCAGAAGGTGATCTGGTTCATCCTGCTGATTGCATGCAGCAGTTACTGGAGAAAGTTAGGCGGCATAGAGTCAATGTTGATGGCAATGTTTGTACTGTCATGGTGACAACATTGGTTCTTGAGGGATGGCAGAGGAAACTTGATCCAGCATACAATGTGATGCATACCTTGCAAACTCTGCTTCTCAAGGCCGACTGGGCAAAGTCCCTTTCATACACAATTGAGGGGCTAATGGCTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

635

Amino Acids

72.58

Weight (kDa)

9.94

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 261 - 525 3.3e-48 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1173
AatII GACGTC 1 cut(s) 1197
Acc36I ACCTGC 2 cut(s) 123, 686
AccI GTMKAC 1 cut(s) 149
AccII CGCG 1 cut(s) 379
AciI CCGC 7 cut(s) 83, 118, 248, 476, 847, 1162, 1726
AclWI GGATC 2 cut(s) 599, 1802
AcoI YGGCCR 2 cut(s) 84, 844
AcsI RAATTY 6 cut(s) 43, 229, 861, 932, 1519, 1529
AcuI CTGAAG 2 cut(s) 99, 1370
AcyI GRCGYC 1 cut(s) 1194
AdeI CACNNNGTG 1 cut(s) 1553
AfaI GTAC 7 cut(s) 137, 188, 497, 682, 1098, 1663, 1759
AfiI CCNNNNNNNGG 4 cut(s) 132, 164, 998, 1141
AgsI TTSAA 8 cut(s) 833, 869, 878, 931, 1295, 1450, 1529, 1537
AjiI CACGTC 1 cut(s) 1550
AjnI CCWGG 5 cut(s) 224, 943, 991, 1359, 1393
AjuI GAANNNNNNNTTGG 2 cut(s) 825, 857
Alw21I GWGCWC 2 cut(s) 533, 1580
Alw26I GTCTC 6 cut(s) 259, 418, 875, 1128, 1201, 1276
Alw44I GTGCAC 1 cut(s) 1576
AlwI GGATC 2 cut(s) 599, 1802
AlwNI CAGNNNCTG 2 cut(s) 701, 1711
Ama87I CYCGRG 1 cut(s) 354
AoxI GGCC 5 cut(s) 84, 112, 171, 844, 1854
ApaLI GTGCAC 1 cut(s) 1576
ApeKI GCWGC 5 cut(s) 728, 853, 1148, 1568, 1702
ApoI RAATTY 6 cut(s) 43, 229, 861, 932, 1519, 1529
AspLEI GCGC 2 cut(s) 381, 1563
AspS9I GGNCC 4 cut(s) 172, 460, 641, 698
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 5 cut(s) 594, 967, 1013, 1685, 1780
AsuNHI GCTAGC 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 354
AvaII GGWCC 3 cut(s) 460, 641, 698
BaeGI GKGCMC 2 cut(s) 439, 1580
BaeI ACNNNNGTAYC 2 cut(s) 931, 964
BanII GRGCYC 1 cut(s) 533
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 830
Bbv12I GWGCWC 2 cut(s) 533, 1580
BbvI GCAGC 5 cut(s) 740, 840, 1135, 1555, 1714
BccI CCATC 6 cut(s) 248, 610, 1179, 1550, 1739, 1785
BceAI ACGGC 1 cut(s) 245
BciT130I CCWGG 5 cut(s) 226, 945, 993, 1361, 1395
BciVI GTATCC 1 cut(s) 1227
BcnI CCSGG 1 cut(s) 399
BcoDI GTCTC 6 cut(s) 259, 418, 875, 1128, 1201, 1276
BfaI CTAG 3 cut(s) 428, 587, 1503
BfmI CTRYAG 1 cut(s) 139
BfuAI ACCTGC 2 cut(s) 123, 686
BfuI GTATCC 1 cut(s) 1227
BglI GCCNNNNNGGC 1 cut(s) 1862
Bme1390I CCNGG 6 cut(s) 226, 399, 945, 993, 1361, 1395
Bme18I GGWCC 3 cut(s) 460, 641, 698
BmeT110I CYCGRG 1 cut(s) 354
BmgBI CACGTC 1 cut(s) 1550
BmgT120I GGNCC 4 cut(s) 172, 460, 641, 698
BmiI GGNNCC 2 cut(s) 621, 1902
BmrFI CCNGG 6 cut(s) 226, 399, 945, 993, 1361, 1395
BmrI ACTGGG 1 cut(s) 1870
BmsI GCATC 6 cut(s) 902, 925, 1237, 1344, 1399, 1815
BmtI GCTAGC 1 cut(s) 431
BmuI ACTGGG 1 cut(s) 1870
BpiI GAAGAC 1 cut(s) 830
BplI GAGNNNNNCTC 2 cut(s) 892, 924
BpmI CTGGAG 2 cut(s) 1650, 1733
BpuEI CTTGAG 5 cut(s) 400, 894, 1110, 1805, 1835
BpuMI CCSGG 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 1194
BsaJI CCNNGG 2 cut(s) 943, 1394
BsaXI ACNNNNNCTCC 2 cut(s) 778, 808
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 164, 998, 1141
Bse1I ACTGG 3 cut(s) 887, 1716, 1865
Bse3DI GCAATG 1 cut(s) 1756
BseBI CCWGG 5 cut(s) 226, 945, 993, 1361, 1395
BseDI CCNNGG 2 cut(s) 943, 1394
BseGI GGATG 7 cut(s) 240, 988, 1093, 1228, 1390, 1684, 1796
BseLI CCNNNNNNNGG 4 cut(s) 132, 164, 998, 1141
BseMI GCAATG 1 cut(s) 1756
BseMII CTCAG 4 cut(s) 563, 659, 1433, 1563
BseNI ACTGG 3 cut(s) 887, 1716, 1865
BseRI GAGGAG 3 cut(s) 37, 180, 1655
BseSI GKGCMC 2 cut(s) 439, 1580
BseXI GCAGC 5 cut(s) 740, 840, 1135, 1555, 1714
BseYI CCCAGC 1 cut(s) 244
BsgI GTGCAG 2 cut(s) 525, 1302
Bsh1236I CGCG 1 cut(s) 379
BshFI GGCC 5 cut(s) 86, 114, 173, 846, 1856
BsiHKAI GWGCWC 2 cut(s) 533, 1580
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 1 cut(s) 398
BslFI GGGAC 1 cut(s) 1855
BslI CCNNNNNNNGG 4 cut(s) 132, 164, 998, 1141
BsmAI GTCTC 6 cut(s) 259, 418, 875, 1128, 1201, 1276
BsmBI CGTCTC 2 cut(s) 1201, 1276
BsmFI GGGAC 1 cut(s) 1855
BsmI GAATGC 2 cut(s) 359, 1271
BsnI GGCC 5 cut(s) 86, 114, 173, 846, 1856
BsoBI CYCGRG 1 cut(s) 354
Bsp1286I GDGCHC 3 cut(s) 439, 533, 1580
Bsp1407I TGTACA 3 cut(s) 186, 495, 1096
Bsp143I GATC 5 cut(s) 591, 742, 1288, 1675, 1807
BspACI CCGC 7 cut(s) 83, 118, 248, 476, 847, 1162, 1726
BspANI GGCC 5 cut(s) 86, 114, 173, 846, 1856
BspCNI CTCAG 4 cut(s) 564, 658, 1432, 1564
BspFNI CGCG 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 621, 1902
BspMI ACCTGC 2 cut(s) 123, 686
BspOI GCTAGC 1 cut(s) 431
BspPI GGATC 2 cut(s) 599, 1802
BsrDI GCAATG 1 cut(s) 1756
BsrGI TGTACA 3 cut(s) 186, 495, 1096
BsrI ACTGG 3 cut(s) 887, 1716, 1865
BssECI CCNNGG 2 cut(s) 943, 1394
BssMI GATC 5 cut(s) 591, 742, 1288, 1675, 1807
BssNAI GTATAC 1 cut(s) 150
BssNI GRCGYC 1 cut(s) 1194
BssSI CACGAG 1 cut(s) 501
Bst1107I GTATAC 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 5 cut(s) 226, 945, 993, 1361, 1395
Bst4CI ACNGT 4 cut(s) 100, 1104, 1446, 1762
Bst6I CTCTTC 2 cut(s) 752, 873
BstACI GRCGYC 1 cut(s) 1194
BstAPI GCANNNNNTGC 4 cut(s) 257, 701, 1834, 1843
BstAUI TGTACA 3 cut(s) 186, 495, 1096
BstC8I GCNNGC 4 cut(s) 116, 429, 851, 1700
BstDEI CTNAG 7 cut(s) 572, 645, 1419, 1572, 1583, 1593, 1905
BstENI CCTNNNNNAGG 1 cut(s) 162
BstF5I GGATG 7 cut(s) 240, 988, 1093, 1228, 1390, 1684, 1796
BstFNI CGCG 1 cut(s) 379
BstHHI GCGC 2 cut(s) 381, 1563
BstKTI GATC 5 cut(s) 594, 745, 1291, 1678, 1810
BstMAI GTCTC 6 cut(s) 259, 418, 875, 1128, 1201, 1276
BstMBI GATC 5 cut(s) 591, 742, 1288, 1675, 1807
BstMWI GCNNNNNNNGC 7 cut(s) 257, 626, 701, 1145, 1834, 1843, 1862
BstNI CCWGG 5 cut(s) 226, 945, 993, 1361, 1395
BstNSI RCATGY 5 cut(s) 521, 1343, 1382, 1390, 1702
BstSCI CCNGG 6 cut(s) 224, 397, 943, 991, 1359, 1393
BstSFI CTRYAG 1 cut(s) 139
BstSLI GKGCMC 2 cut(s) 439, 1580
BstUI CGCG 1 cut(s) 379
BstV1I GCAGC 5 cut(s) 740, 840, 1135, 1555, 1714
BstV2I GAAGAC 1 cut(s) 830
BstX2I RGATCY 1 cut(s) 591
BstYI RGATCY 1 cut(s) 591
BstZ17I GTATAC 1 cut(s) 150
BsuI GTATCC 1 cut(s) 1227
BsuRI GGCC 5 cut(s) 86, 114, 173, 846, 1856
BtrI CACGTC 1 cut(s) 1550
BtsCI GGATG 7 cut(s) 240, 988, 1093, 1228, 1390, 1684, 1796
BtsI GCAGTG 1 cut(s) 1563
BtsIMutI CAGTG 2 cut(s) 75, 1563
BveI ACCTGC 2 cut(s) 123, 686
Cac8I GCNNGC 4 cut(s) 116, 429, 851, 1700
CaiI CAGNNNCTG 2 cut(s) 701, 1711
CfoI GCGC 2 cut(s) 381, 1563
Cfr13I GGNCC 4 cut(s) 172, 460, 641, 698
CseI GACGC 1 cut(s) 1258
Csp6I GTAC 7 cut(s) 136, 187, 496, 681, 1097, 1662, 1758
CviQI GTAC 7 cut(s) 136, 187, 496, 681, 1097, 1662, 1758
DdeI CTNAG 7 cut(s) 572, 645, 1419, 1572, 1583, 1593, 1905
DpnI GATC 5 cut(s) 593, 744, 1290, 1677, 1809
DpnII GATC 5 cut(s) 591, 742, 1288, 1675, 1807
DraI TTTAAA 1 cut(s) 1071
DraIII CACNNNGTG 1 cut(s) 1553
EaeI YGGCCR 2 cut(s) 84, 844
Eam1104I CTCTTC 2 cut(s) 752, 873
EarI CTCTTC 2 cut(s) 752, 873
Ecl136II GAGCTC 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 533
Eco47I GGWCC 3 cut(s) 460, 641, 698
Eco53kI GAGCTC 1 cut(s) 531
Eco57I CTGAAG 2 cut(s) 99, 1370
Eco88I CYCGRG 1 cut(s) 354
EcoICRI GAGCTC 1 cut(s) 531
EcoNI CCTNNNNNAGG 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 5 cut(s) 224, 943, 991, 1359, 1393
EcoT22I ATGCAT 2 cut(s) 1392, 1830
EcoT38I GRGCYC 1 cut(s) 533
Esp3I CGTCTC 2 cut(s) 1201, 1276
FalI AAGNNNNNCTT 2 cut(s) 1119, 1151
FaqI GGGAC 1 cut(s) 1855
FblI GTMKAC 1 cut(s) 149
FokI GGATG 7 cut(s) 227, 975, 1100, 1215, 1377, 1671, 1803
FriOI GRGCYC 1 cut(s) 533
FspBI CTAG 3 cut(s) 428, 587, 1503
GlaI GCGC 2 cut(s) 380, 1562
GsaI CCCAGC 1 cut(s) 248
GsuI CTGGAG 2 cut(s) 1650, 1733
HaeIII GGCC 5 cut(s) 86, 114, 173, 846, 1856
HapII CCGG 1 cut(s) 398
HgaI GACGC 1 cut(s) 1258
HhaI GCGC 2 cut(s) 381, 1563
Hin1I GRCGYC 1 cut(s) 1194
Hin6I GCGC 2 cut(s) 379, 1561
HinP1I GCGC 2 cut(s) 379, 1561
HincII GTYRAC 2 cut(s) 950, 1132
HindII GTYRAC 2 cut(s) 950, 1132
HindIII AAGCTT 1 cut(s) 1620
HinfI GANTC 6 cut(s) 177, 632, 1004, 1587, 1643, 1734
HpaII CCGG 1 cut(s) 398
HphI GGTGA 5 cut(s) 594, 967, 1013, 1685, 1780
Hpy166II GTNNAC 8 cut(s) 150, 498, 641, 950, 1097, 1132, 1366, 1578
Hpy188I TCNGA 6 cut(s) 213, 301, 778, 1350, 1422, 1437
Hpy188III TCNNGA 7 cut(s) 6, 740, 797, 1503, 1553, 1667, 1784
Hpy8I GTNNAC 8 cut(s) 150, 498, 641, 950, 1097, 1132, 1366, 1578
Hpy99I CGWCG 1 cut(s) 1554
HpyAV CCTTC 5 cut(s) 451, 548, 1183, 1440, 1664
HpyCH4III ACNGT 4 cut(s) 100, 1104, 1446, 1762
HpyCH4IV ACGT 4 cut(s) 17, 1194, 1250, 1549
HpyF10VI GCNNNNNNNGC 7 cut(s) 257, 626, 701, 1145, 1834, 1843, 1862
HpyF3I CTNAG 7 cut(s) 572, 645, 1419, 1572, 1583, 1593, 1905
HpySE526I ACGT 4 cut(s) 17, 1194, 1250, 1549
Hsp92I GRCGYC 1 cut(s) 1194
HspAI GCGC 2 cut(s) 379, 1561
Kzo9I GATC 5 cut(s) 591, 742, 1288, 1675, 1807
LmnI GCTCC 4 cut(s) 625, 799, 1362, 1906
Lsp1109I GCAGC 5 cut(s) 740, 840, 1135, 1555, 1714
LweI GCATC 6 cut(s) 902, 925, 1237, 1344, 1399, 1815
MaeI CTAG 3 cut(s) 428, 587, 1503
MaeII ACGT 4 cut(s) 17, 1194, 1250, 1549
MaeIII GTNAC 4 cut(s) 52, 1508, 1707, 1768
MalI GATC 5 cut(s) 593, 744, 1290, 1677, 1809
MboI GATC 5 cut(s) 591, 742, 1288, 1675, 1807
MboII GAAGA 6 cut(s) 668, 739, 835, 890, 1283, 1363
MfeI CAATTG 2 cut(s) 828, 1884
MflI RGATCY 1 cut(s) 591
MhlI GDGCHC 3 cut(s) 439, 533, 1580
MlyI GAGTC 3 cut(s) 1581, 1652, 1743
Mph1103I ATGCAT 2 cut(s) 1392, 1830
MseI TTAA 3 cut(s) 978, 1070, 1311
MslI CAYNNNNRTG 2 cut(s) 614, 993
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 1 cut(s) 398
MspR9I CCNGG 6 cut(s) 226, 399, 945, 993, 1361, 1395
MunI CAATTG 2 cut(s) 828, 1884
Mva1269I GAATGC 2 cut(s) 359, 1271
MvaI CCWGG 5 cut(s) 226, 945, 993, 1361, 1395
MvnI CGCG 1 cut(s) 379
MwoI GCNNNNNNNGC 7 cut(s) 257, 626, 701, 1145, 1834, 1843, 1862
NciI CCSGG 1 cut(s) 399
NdeII GATC 5 cut(s) 591, 742, 1288, 1675, 1807
NheI GCTAGC 1 cut(s) 427
NlaIV GGNNCC 2 cut(s) 621, 1902
NmuCI GTSAC 3 cut(s) 52, 1508, 1768
NsiI ATGCAT 2 cut(s) 1392, 1830
NspI RCATGY 5 cut(s) 521, 1343, 1382, 1390, 1702
PaeI GCATGC 1 cut(s) 1702
PaeR7I CTCGAG 1 cut(s) 354
PctI GAATGC 2 cut(s) 359, 1271
PfeI GAWTC 3 cut(s) 177, 632, 1004
PfoI TCCNGGA 1 cut(s) 224
PleI GAGTC 3 cut(s) 1581, 1651, 1742
PpsI GAGTC 3 cut(s) 1581, 1651, 1742
PpuMI RGGWCCY 1 cut(s) 698
PsiI TTATAA 1 cut(s) 1173
Psp124BI GAGCTC 1 cut(s) 533
Psp5II RGGWCCY 1 cut(s) 698
Psp6I CCWGG 5 cut(s) 224, 943, 991, 1359, 1393
PspFI CCCAGC 1 cut(s) 244
PspGI CCWGG 5 cut(s) 224, 943, 991, 1359, 1393
PspN4I GGNNCC 2 cut(s) 621, 1902
PspPI GGNCC 4 cut(s) 172, 460, 641, 698
PspPPI RGGWCCY 1 cut(s) 698
PspXI VCTCGAGB 1 cut(s) 354
PstNI CAGNNNCTG 2 cut(s) 701, 1711
PsuI RGATCY 1 cut(s) 591
RsaI GTAC 7 cut(s) 137, 188, 497, 682, 1098, 1663, 1759
RsaNI GTAC 7 cut(s) 136, 187, 496, 681, 1097, 1662, 1758
RseI CAYNNNNRTG 2 cut(s) 614, 993
SacI GAGCTC 1 cut(s) 533
SaqAI TTAA 3 cut(s) 978, 1070, 1311
Sau3AI GATC 5 cut(s) 591, 742, 1288, 1675, 1807
Sau96I GGNCC 4 cut(s) 172, 460, 641, 698
SchI GAGTC 3 cut(s) 1581, 1652, 1743
ScrFI CCNGG 6 cut(s) 226, 399, 945, 993, 1361, 1395
SduI GDGCHC 3 cut(s) 439, 533, 1580
SfaNI GCATC 6 cut(s) 902, 925, 1237, 1344, 1399, 1815
SfcI CTRYAG 1 cut(s) 139
Sfr274I CTCGAG 1 cut(s) 354
SinI GGWCC 3 cut(s) 460, 641, 698
SlaI CTCGAG 1 cut(s) 354
SmiMI CAYNNNNRTG 2 cut(s) 614, 993
SmlI CTYRAG 6 cut(s) 354, 415, 909, 1125, 1784, 1850
SmoI CTYRAG 6 cut(s) 354, 415, 909, 1125, 1784, 1850
SphI GCATGC 1 cut(s) 1702
SsiI CCGC 7 cut(s) 83, 118, 248, 476, 847, 1162, 1726
SspMI CTAG 3 cut(s) 428, 587, 1503
SstI GAGCTC 1 cut(s) 533
StyD4I CCNGG 6 cut(s) 224, 397, 943, 991, 1359, 1393
TaaI ACNGT 4 cut(s) 100, 1104, 1446, 1762
TaiI ACGT 4 cut(s) 20, 1197, 1253, 1552
TaqI TCGA 3 cut(s) 355, 937, 1411
TatI WGTACW 4 cut(s) 186, 495, 1096, 1757
TauI GCSGC 6 cut(s) 86, 121, 251, 849, 1164, 1729
TfiI GAWTC 3 cut(s) 177, 632, 1004
Tru1I TTAA 3 cut(s) 978, 1070, 1311
Tru9I TTAA 3 cut(s) 978, 1070, 1311
TscAI CASTG 2 cut(s) 82, 1570
TseFI GTSAC 3 cut(s) 52, 1508, 1768
TseI GCWGC 5 cut(s) 728, 853, 1148, 1568, 1702
Tsp45I GTSAC 3 cut(s) 52, 1508, 1768
TspDTI ATGAA 8 cut(s) 697, 773, 1048, 1104, 1317, 1615, 1673, 1866
TspRI CASTG 2 cut(s) 82, 1570
VneI GTGCAC 1 cut(s) 1576
VpaK11BI GGWCC 3 cut(s) 460, 641, 698
XagI CCTNNNNNAGG 1 cut(s) 162
XapI RAATTY 6 cut(s) 43, 229, 861, 932, 1519, 1529
XbaI TCTAGA 1 cut(s) 1502
XceI RCATGY 5 cut(s) 521, 1343, 1382, 1390, 1702
XhoI CTCGAG 1 cut(s) 354
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 3 cut(s) 428, 587, 1503
ZraI GACGTC 1 cut(s) 1195
Zsp2I ATGCAT 2 cut(s) 1392, 1830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.