Prupe.8G028600_v2.0.a1

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0003891
Physical Location & Seq
Forward (+)
2587053 .. 2588453
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G028600.1

Sequence Viewer

Length: 1386 bp
ATGAGAACCAAAAGATGTTCAAACGTTGCTTCTGAGACAGATGAGATGTTCGGATTCCGTTCAGGATCAGACTTTACATTTGATGAGTTTCAGAGATATGCCTACACTTTCAAGGAGAGTTACTTTGGAATACAGGATGCTAAGGAGGGTTCAAATGCTGGTGAAACTAAAAAGAAAACATGGAAACCCTCTGTGGAGGATATTGAAGGTGAATACTGGCGAATAGTTGAGCAACCAACAGATGAGGTTGAGGTTTATTATGGAGCTGACTTGGAAACAGGAGTATTTGGAAGTGGGTTTCCTAAGGCATTGTCTGTGGTAACCGGAAATGATTCGGATCAGTATGCAATGTCAGGTTGGAATCTAAATCACTTCCCACGTCTGCCCGGTTCTGTACTAAGTTTTAAAATCAATTCTAGTCCCATGGTCTATGTGGAGATGTGCTTTTCTTCATTTTGTTGGCTTGTTGAGGACCACCACCTCTATAGTCTATTCCCTAACTGGGGTGATCCAAAAGTATGTTATGGAGTGTCTGGAAGCCATGCTCCCGCTTTGGAACGTGCAATGAGAAAGCATTTGCCCAATTTGTTTGAGGAACAACCTCATTTACTCAATGAACTGGTCACTCAGCTATCTCCTTCAGTTTTAAAGTCTGAAGGTGTTCCTTTGCATAGAGCTGTCCAACATTCTGGGGAGTTTGTTCTTACTTTTCCGAGGGCATACCATTCTGGATTTAATTGTGGCTTTAACTGTGCTGAGGCAGTCAACGTGGCCCCTGTTGATTGGTTAGAACATGGGCAAAATGCAGTTGAACTCTACAGTAAGCAATGTCGCAAGACATCAACTTCACATGGCAAATTTTTACTGGGATCAGCTCGGGAAGCTGTACAGGCCCTTTGGGAACTATCAGTTCTTGGAAAGAAGACTACAAGAAATATGAGCTGGCAAAGTGTTTGTGGCAAAGATGGTATGCTCACGAGAGCAGTTAAGACGCGAGTGCGAATGTTGGAGGAAAGACTAGATAGTCTTCCAATTTGTTTGAAGCCGCAAAAAATGGAAAGAGACTTTGATTTGAACAATGGGAGAGAATGCTTTTCTTGCTTCTATGACTTACACTTGTTGGCAGCTCGCTGCAAGTGCTCTTCTGACCGATTTTCATGTCTCAAACATGCAAAGCTTTTTTGTTCATGTGACATCAATCATAGATATGTCCTTCAACTTCACACTATCAATGAACTAAATATGCTAGTTGAAGCATTGGAAGGGAGATCAGAAGCCATAATATGGGCATCCGAGGACACTACTGATATGCATACAACTAGACTGGATCATGAAAGTGGCATGTTTCATAAAAGAATCAAGTCTTGTGATCCAAGGGAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

462

Amino Acids

52.47

Weight (kDa)

6.15

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1023
AccB7I CCANNNNNTGG 1 cut(s) 1284
AccII CGCG 1 cut(s) 994
AciI CCGC 2 cut(s) 549, 1046
AclI AACGTT 1 cut(s) 24
AclWI GGATC 6 cut(s) 73, 345, 503, 877, 1335, 1364
AcsI RAATTY 1 cut(s) 857
AcuI CTGAAG 2 cut(s) 624, 675
AfaI GTAC 2 cut(s) 396, 888
AfiI CCNNNNNNNGG 3 cut(s) 502, 503, 1284
AgsI TTSAA 9 cut(s) 21, 112, 153, 206, 812, 1042, 1075, 1217, 1253
AjiI CACGTC 1 cut(s) 380
AluBI AGCT 8 cut(s) 266, 631, 677, 875, 884, 942, 1127, 1177
AluI AGCT 8 cut(s) 266, 631, 677, 875, 884, 942, 1127, 1177
Alw21I GWGCWC 1 cut(s) 1142
Alw26I GTCTC 3 cut(s) 29, 1056, 1166
AlwI GGATC 6 cut(s) 73, 345, 503, 877, 1335, 1364
Ama87I CYCGRG 1 cut(s) 876
AoxI GGCC 2 cut(s) 771, 891
ApeKI GCWGC 2 cut(s) 1124, 1131
ApoI RAATTY 1 cut(s) 857
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
Asp700I GAANNNNTTC 2 cut(s) 331, 660
AspS9I GGNCC 3 cut(s) 472, 772, 892
AsuC2I CCSGG 1 cut(s) 387
AsuHPI GGTGA 3 cut(s) 173, 221, 518
AvaI CYCGRG 1 cut(s) 876
AvaII GGWCC 1 cut(s) 472
AxyI CCTNAGG 1 cut(s) 303
BauI CACGAG 1 cut(s) 976
BbsI GAAGAC 2 cut(s) 929, 1019
Bbv12I GWGCWC 1 cut(s) 1142
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 2 cut(s) 1118, 1136
BccI CCATC 1 cut(s) 959
BcnI CCSGG 1 cut(s) 387
BcoDI GTCTC 3 cut(s) 29, 1056, 1166
BfaI CTAG 4 cut(s) 417, 1019, 1247, 1320
BfmI CTRYAG 2 cut(s) 484, 817
BisI GCNGC 3 cut(s) 1046, 1125, 1132
BlsI GCNGC 3 cut(s) 1047, 1126, 1133
Bme1390I CCNGG 1 cut(s) 387
Bme18I GGWCC 1 cut(s) 472
BmeT110I CYCGRG 1 cut(s) 876
BmgBI CACGTC 1 cut(s) 380
BmgT120I GGNCC 3 cut(s) 472, 772, 892
BmiI GGNNCC 1 cut(s) 774
BmrFI CCNGG 1 cut(s) 387
BmrI ACTGGG 2 cut(s) 511, 875
BmsI GCATC 2 cut(s) 127, 1298
BmuI ACTGGG 2 cut(s) 511, 875
BpiI GAAGAC 2 cut(s) 929, 1019
Bpu10I CCTNAGC 2 cut(s) 141, 756
BpuMI CCSGG 1 cut(s) 387
BsaBI GATNNNNATC 1 cut(s) 336
BsaJI CCNNGG 4 cut(s) 423, 713, 1293, 1373
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 3 cut(s) 502, 503, 1284
Bse1I ACTGG 5 cut(s) 221, 506, 624, 870, 1329
Bse21I CCTNAGG 1 cut(s) 303
Bse3DI GCAATG 3 cut(s) 354, 570, 833
Bse8I GATNNNNATC 1 cut(s) 336
BseDI CCNNGG 4 cut(s) 423, 713, 1293, 1373
BseGI GGATG 2 cut(s) 142, 1289
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 3 cut(s) 502, 503, 1284
BseMI GCAATG 3 cut(s) 354, 570, 833
BseMII CTCAG 3 cut(s) 24, 641, 747
BseNI ACTGG 5 cut(s) 221, 506, 624, 870, 1329
BseXI GCAGC 2 cut(s) 1118, 1136
Bsh1236I CGCG 1 cut(s) 994
BshFI GGCC 2 cut(s) 773, 893
BsiHKAI GWGCWC 1 cut(s) 1142
BsiHKCI CYCGRG 1 cut(s) 876
BsiSI CCGG 2 cut(s) 324, 387
BslFI GGGAC 1 cut(s) 405
BslI CCNNNNNNNGG 3 cut(s) 502, 503, 1284
BsmAI GTCTC 3 cut(s) 29, 1056, 1166
BsmFI GGGAC 1 cut(s) 405
BsmI GAATGC 1 cut(s) 1094
BsnI GGCC 2 cut(s) 773, 893
BsoBI CYCGRG 1 cut(s) 876
Bsp1286I GDGCHC 1 cut(s) 1142
Bsp1407I TGTACA 1 cut(s) 886
Bsp143I GATC 7 cut(s) 65, 337, 508, 869, 1268, 1327, 1369
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 2 cut(s) 549, 1046
BspANI GGCC 2 cut(s) 773, 893
BspCNI CTCAG 3 cut(s) 25, 640, 748
BspFNI CGCG 1 cut(s) 994
BspHI TCATGA 1 cut(s) 1330
BspLI GGNNCC 1 cut(s) 774
BspPI GGATC 6 cut(s) 73, 345, 503, 877, 1335, 1364
BspQI GCTCTTC 1 cut(s) 1147
BsrDI GCAATG 3 cut(s) 354, 570, 833
BsrGI TGTACA 1 cut(s) 886
BsrI ACTGG 5 cut(s) 221, 506, 624, 870, 1329
BssECI CCNNGG 4 cut(s) 423, 713, 1293, 1373
BssMI GATC 7 cut(s) 65, 337, 508, 869, 1268, 1327, 1369
BssSI CACGAG 1 cut(s) 976
BssT1I CCWWGG 2 cut(s) 423, 1373
Bst2BI CACGAG 1 cut(s) 976
Bst4CI ACNGT 2 cut(s) 752, 821
Bst6I CTCTTC 1 cut(s) 1147
BstAUI TGTACA 1 cut(s) 886
BstC8I GCNNGC 2 cut(s) 944, 1129
BstDEI CTNAG 6 cut(s) 33, 141, 303, 398, 627, 756
BstDSI CCRYGG 1 cut(s) 423
BstEII GGTNACC 1 cut(s) 319
BstF5I GGATG 2 cut(s) 142, 1289
BstFNI CGCG 1 cut(s) 994
BstKTI GATC 7 cut(s) 68, 340, 511, 872, 1271, 1330, 1372
BstMAI GTCTC 3 cut(s) 29, 1056, 1166
BstMBI GATC 7 cut(s) 65, 337, 508, 869, 1268, 1327, 1369
BstMWI GCNNNNNNNGC 4 cut(s) 881, 890, 1098, 1137
BstNSI RCATGY 2 cut(s) 1172, 1345
BstPI GGTNACC 1 cut(s) 319
BstSCI CCNGG 1 cut(s) 385
BstSFI CTRYAG 2 cut(s) 484, 817
BstUI CGCG 1 cut(s) 994
BstV1I GCAGC 2 cut(s) 1118, 1136
BstV2I GAAGAC 2 cut(s) 929, 1019
BstXI CCANNNNNNTGG 1 cut(s) 689
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 2 cut(s) 773, 893
BtgI CCRYGG 1 cut(s) 423
BtrI CACGTC 1 cut(s) 380
BtsCI GGATG 2 cut(s) 142, 1289
Cac8I GCNNGC 2 cut(s) 944, 1129
CciI TCATGA 1 cut(s) 1330
Cfr13I GGNCC 3 cut(s) 472, 772, 892
CseI GACGC 1 cut(s) 1000
Csp6I GTAC 2 cut(s) 395, 887
CviQI GTAC 2 cut(s) 395, 887
DdeI CTNAG 6 cut(s) 33, 141, 303, 398, 627, 756
DpnI GATC 7 cut(s) 67, 339, 510, 871, 1270, 1329, 1371
DpnII GATC 7 cut(s) 65, 337, 508, 869, 1268, 1327, 1369
DraI TTTAAA 2 cut(s) 406, 648
DrdI GACNNNNNNGTC 1 cut(s) 1023
DseDI GACNNNNNNGTC 1 cut(s) 1023
Eam1104I CTCTTC 1 cut(s) 1147
EarI CTCTTC 1 cut(s) 1147
Eco130I CCWWGG 2 cut(s) 423, 1373
Eco47I GGWCC 1 cut(s) 472
Eco57I CTGAAG 2 cut(s) 624, 675
Eco81I CCTNAGG 1 cut(s) 303
Eco88I CYCGRG 1 cut(s) 876
Eco91I GGTNACC 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 892
EcoO65I GGTNACC 1 cut(s) 319
EcoT14I CCWWGG 2 cut(s) 423, 1373
EcoT22I ATGCAT 1 cut(s) 1314
ErhI CCWWGG 2 cut(s) 423, 1373
FalI AAGNNNNNCTT 1 cut(s) 1366
FaqI GGGAC 1 cut(s) 405
FauI CCCGC 1 cut(s) 556
Fnu4HI GCNGC 3 cut(s) 1046, 1125, 1132
FokI GGATG 2 cut(s) 149, 1276
Fsp4HI GCNGC 3 cut(s) 1046, 1125, 1132
FspBI CTAG 4 cut(s) 417, 1019, 1247, 1320
GluI GCNGC 3 cut(s) 1046, 1125, 1132
HaeIII GGCC 2 cut(s) 773, 893
HapII CCGG 2 cut(s) 324, 387
HgaI GACGC 1 cut(s) 1000
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HindIII AAGCTT 1 cut(s) 1175
HinfI GANTC 4 cut(s) 54, 332, 361, 1356
HpaII CCGG 2 cut(s) 324, 387
HphI GGTGA 3 cut(s) 173, 221, 518
Hpy166II GTNNAC 1 cut(s) 766
Hpy188III TCNNGA 6 cut(s) 63, 534, 729, 878, 976, 1331
Hpy8I GTNNAC 1 cut(s) 766
HpyAV CCTTC 5 cut(s) 200, 648, 650, 1223, 1256
HpyCH4III ACNGT 2 cut(s) 752, 821
HpyCH4IV ACGT 4 cut(s) 24, 379, 559, 768
HpyCH4V TGCA 7 cut(s) 347, 563, 670, 806, 1134, 1172, 1312
HpyF10VI GCNNNNNNNGC 4 cut(s) 881, 890, 1098, 1137
HpyF3I CTNAG 6 cut(s) 33, 141, 303, 398, 627, 756
HpySE526I ACGT 4 cut(s) 24, 379, 559, 768
Kzo9I GATC 7 cut(s) 65, 337, 508, 869, 1268, 1327, 1369
LguI GCTCTTC 1 cut(s) 1147
LmnI GCTCC 2 cut(s) 263, 550
Lsp1109I GCAGC 2 cut(s) 1118, 1136
LweI GCATC 2 cut(s) 127, 1298
MaeI CTAG 4 cut(s) 417, 1019, 1247, 1320
MaeII ACGT 4 cut(s) 24, 379, 559, 768
MaeIII GTNAC 4 cut(s) 119, 319, 622, 1190
MalI GATC 7 cut(s) 67, 339, 510, 871, 1270, 1329, 1371
MboI GATC 7 cut(s) 65, 337, 508, 869, 1268, 1327, 1369
MboII GAAGA 4 cut(s) 441, 934, 1019, 1134
MhlI GDGCHC 1 cut(s) 1142
MluCI AATT 5 cut(s) 412, 583, 736, 857, 1032
MmeI TCCRAC 3 cut(s) 338, 706, 987
Mph1103I ATGCAT 1 cut(s) 1314
MroXI GAANNNNTTC 2 cut(s) 331, 660
MseI TTAA 5 cut(s) 405, 647, 735, 747, 987
MslI CAYNNNNRTG 2 cut(s) 1206, 1335
MspI CCGG 2 cut(s) 324, 387
MspR9I CCNGG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 1094
MvnI CGCG 1 cut(s) 994
MwoI GCNNNNNNNGC 4 cut(s) 881, 890, 1098, 1137
NciI CCSGG 1 cut(s) 387
NcoI CCATGG 1 cut(s) 423
NdeII GATC 7 cut(s) 65, 337, 508, 869, 1268, 1327, 1369
NlaIV GGNNCC 1 cut(s) 774
NmuCI GTSAC 2 cut(s) 622, 1190
NsiI ATGCAT 1 cut(s) 1314
NspI RCATGY 2 cut(s) 1172, 1345
PagI TCATGA 1 cut(s) 1330
PciSI GCTCTTC 1 cut(s) 1147
PctI GAATGC 1 cut(s) 1094
PdmI GAANNNNTTC 2 cut(s) 331, 660
PfeI GAWTC 4 cut(s) 54, 332, 361, 1356
PflMI CCANNNNNTGG 1 cut(s) 1284
PkrI GCNGC 3 cut(s) 1047, 1126, 1133
Psp1406I AACGTT 1 cut(s) 24
PspEI GGTNACC 1 cut(s) 319
PspN4I GGNNCC 1 cut(s) 774
PspPI GGNCC 3 cut(s) 472, 772, 892
RsaI GTAC 2 cut(s) 396, 888
RsaNI GTAC 2 cut(s) 395, 887
RseI CAYNNNNRTG 2 cut(s) 1206, 1335
SapI GCTCTTC 1 cut(s) 1147
SaqAI TTAA 5 cut(s) 405, 647, 735, 747, 987
SatI GCNGC 3 cut(s) 1046, 1125, 1132
Sau3AI GATC 7 cut(s) 65, 337, 508, 869, 1268, 1327, 1369
Sau96I GGNCC 3 cut(s) 472, 772, 892
ScrFI CCNGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 1142
SfaNI GCATC 2 cut(s) 127, 1298
SfcI CTRYAG 2 cut(s) 484, 817
SinI GGWCC 1 cut(s) 472
SmiMI CAYNNNNRTG 2 cut(s) 1206, 1335
Sse9I AATT 5 cut(s) 412, 583, 736, 857, 1032
SsiI CCGC 2 cut(s) 549, 1046
SspMI CTAG 4 cut(s) 417, 1019, 1247, 1320
StyD4I CCNGG 1 cut(s) 385
StyI CCWWGG 2 cut(s) 423, 1373
TaaI ACNGT 2 cut(s) 752, 821
TaiI ACGT 4 cut(s) 27, 382, 562, 771
TaqII GACCGA 1 cut(s) 1164
TasI AATT 5 cut(s) 412, 583, 736, 857, 1032
TatI WGTACW 2 cut(s) 394, 886
TauI GCSGC 1 cut(s) 1048
TfiI GAWTC 4 cut(s) 54, 332, 361, 1356
Tru1I TTAA 5 cut(s) 405, 647, 735, 747, 987
Tru9I TTAA 5 cut(s) 405, 647, 735, 747, 987
TseFI GTSAC 2 cut(s) 622, 1190
TseI GCWGC 2 cut(s) 1124, 1131
Tsp45I GTSAC 2 cut(s) 622, 1190
TspDTI ATGAA 7 cut(s) 441, 630, 1146, 1176, 1248, 1337, 1347
TspGWI ACGGA 1 cut(s) 47
Van91I CCANNNNNTGG 1 cut(s) 1284
VpaK11BI GGWCC 1 cut(s) 472
XapI RAATTY 1 cut(s) 857
XceI RCATGY 2 cut(s) 1172, 1345
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XmnI GAANNNNTTC 2 cut(s) 331, 660
XspI CTAG 4 cut(s) 417, 1019, 1247, 1320
Zsp2I ATGCAT 1 cut(s) 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.