pycom05g24730

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003891
Physical Location & Seq
Reverse (-)
26252929 .. 26254245
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g24730.1

Sequence Viewer

Length: 894 bp
ATGGGAGCCAGAAGACGTTCAGAAGCTAATGCTGATATTGAGGCCGATGAGAAGTTTGGGTTCCATTCAGGGTCGGACTTTACATTTGAAGAGTTTCAGAAACATGCTGCTACTTTTAAGGAGAGTTACTTCGGAACAAAGAATGTCAAGGAGGGTTCAACTCGTGGTGAAACTAAAAGAAAAACATGGGCACCCTCTGTGGAGGATATCGAAGGTGAATACTGGCGAATAGTTGAGATACCAACAACAGATGGGTTGAGTGGTTTTCCTAAGGCATCATCAACCGGAAGTGATTCGGAGAAGTATGCATTGTCAGGTTGGAATCTAAATAACTTCCCACGTCTGCCTGGTTCTGCACTGTGTTTTGAAGCAAGCGATATCTCGGGAGTTCTAGTCCCATGGCTCTATGTTGGGATGTGCTTTTCTTCATTTTGTTGGTATCATGTTGAGGACCACCACCGCTATTCGCTAAATTATCTGCACTGGGGTGACCCAAAAGGATGGTATGGAGTCTCTGGAAGCCATGCAATGGATTTGGAACGTACAATGAGAGCGTATTTGCCTGATCTGTTCGAGGAACAACCTGATTTACTCAATGAACTGCTATCTCCTACAGTTTTAAGGTCTGAAGGAGTACCTGTGCATCGAGCCGTCCAGCATTCTGGGGAGTTTGTTCTTACCCTCCCGCGGGCATACCATTCTGGATTTAATTGTGGTTGTGATGAGGCAATGAACGTGGCCCCTGTTGATTGGTTAGAACATGGGCAAAATGCAGTTGAGCTCTACAGTGAGCAGTGCCCTCTGGGAAAAATCAGTTTTTCGAAAGAAAACTACAAGAAATATGAGCTGGCAAAGTGTTTGTGGCAAATATGGTCTACTCACCAGAGCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

298

Amino Acids

33.68

Weight (kDa)

5.38

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjC PF02373 134 - 240 6.1e-31 JmjC domain, hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 190
AccB7I CCANNNNNTGG 1 cut(s) 529
AccI GTMKAC 1 cut(s) 875
AccII CGCG 1 cut(s) 688
AciI CCGC 3 cut(s) 460, 686, 688
AcuI CTGAAG 1 cut(s) 648
AfaI GTAC 2 cut(s) 544, 636
AfiI CCNNNNNNNGG 3 cut(s) 529, 687, 688
AgsI TTSAA 3 cut(s) 89, 159, 368
AjiI CACGTC 1 cut(s) 341
AjnI CCWGG 1 cut(s) 346
AleI CACNNNNGTG 1 cut(s) 486
AluBI AGCT 3 cut(s) 26, 781, 847
AluI AGCT 3 cut(s) 26, 781, 847
Alw21I GWGCWC 1 cut(s) 783
Alw26I GTCTC 1 cut(s) 517
Ama87I CYCGRG 1 cut(s) 382
AoxI GGCC 2 cut(s) 42, 738
ApeKI GCWGC 1 cut(s) 107
ArsI GACNNNNNNTTYG 2 cut(s) 68, 100
Asp700I GAANNNNTTC 3 cut(s) 16, 93, 292
AspS9I GGNCC 2 cut(s) 451, 739
AsuHPI GGTGA 4 cut(s) 179, 227, 500, 872
AsuII TTCGAA 1 cut(s) 821
AvaI CYCGRG 1 cut(s) 382
AvaII GGWCC 1 cut(s) 451
AxyI CCTNAGG 1 cut(s) 270
BaeGI GKGCMC 2 cut(s) 193, 800
BanI GGYRCC 1 cut(s) 190
BanII GRGCYC 1 cut(s) 783
BauI CACGAG 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 783
BbvI GCAGC 1 cut(s) 94
BccI CCATC 2 cut(s) 245, 495
BceAI ACGGC 1 cut(s) 635
BciT130I CCWGG 1 cut(s) 348
BcoDI GTCTC 1 cut(s) 517
BfaI CTAG 1 cut(s) 392
BfmI CTRYAG 2 cut(s) 612, 784
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 1 cut(s) 451
BmeT110I CYCGRG 1 cut(s) 382
BmgBI CACGTC 1 cut(s) 341
BmgT120I GGNCC 2 cut(s) 451, 739
BmiI GGNNCC 4 cut(s) 7, 62, 192, 741
BmrFI CCNGG 1 cut(s) 348
BmrI ACTGGG 1 cut(s) 493
BmsI GCATC 2 cut(s) 284, 652
BmuI ACTGGG 1 cut(s) 493
BpiI GAAGAC 1 cut(s) 19
Bpu14I TTCGAA 1 cut(s) 821
BsaJI CCNNGG 2 cut(s) 398, 686
BsaWI WCCGGW 1 cut(s) 284
BsaXI ACNNNNNCTCC 4 cut(s) 378, 408, 624, 654
Bsc4I CCNNNNNNNGG 3 cut(s) 529, 687, 688
Bse1I ACTGG 2 cut(s) 227, 488
Bse21I CCTNAGG 1 cut(s) 270
Bse3DI GCAATG 2 cut(s) 534, 735
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 398, 686
BseGI GGATG 2 cut(s) 420, 506
BseLI CCNNNNNNNGG 3 cut(s) 529, 687, 688
BseMI GCAATG 2 cut(s) 534, 735
BseNI ACTGG 2 cut(s) 227, 488
BseSI GKGCMC 2 cut(s) 193, 800
BseXI GCAGC 1 cut(s) 94
BsgI GTGCAG 2 cut(s) 339, 464
Bsh1236I CGCG 1 cut(s) 688
BshFI GGCC 2 cut(s) 44, 740
BshNI GGYRCC 1 cut(s) 190
BsiHKAI GWGCWC 1 cut(s) 783
BsiHKCI CYCGRG 1 cut(s) 382
BsiSI CCGG 1 cut(s) 285
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 3 cut(s) 529, 687, 688
BsmAI GTCTC 1 cut(s) 517
BsmFI GGGAC 1 cut(s) 380
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 2 cut(s) 44, 740
BsoBI CYCGRG 1 cut(s) 382
Bsp119I TTCGAA 1 cut(s) 821
Bsp1286I GDGCHC 3 cut(s) 193, 783, 800
Bsp143I GATC 1 cut(s) 565
Bsp19I CCATGG 1 cut(s) 398
BspACI CCGC 3 cut(s) 460, 686, 688
BspANI GGCC 2 cut(s) 44, 740
BspFNI CGCG 1 cut(s) 688
BspLI GGNNCC 4 cut(s) 7, 62, 192, 741
BspT104I TTCGAA 1 cut(s) 821
BspT107I GGYRCC 1 cut(s) 190
BsrDI GCAATG 2 cut(s) 534, 735
BsrI ACTGG 2 cut(s) 227, 488
BssECI CCNNGG 2 cut(s) 398, 686
BssMI GATC 1 cut(s) 565
BssSI CACGAG 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 398
Bst2BI CACGAG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 3 cut(s) 360, 616, 788
Bst6I CTCTTC 1 cut(s) 84
BstBI TTCGAA 1 cut(s) 821
BstC8I GCNNGC 3 cut(s) 373, 690, 849
BstDEI CTNAG 1 cut(s) 270
BstDSI CCRYGG 2 cut(s) 398, 686
BstEII GGTNACC 1 cut(s) 488
BstF5I GGATG 2 cut(s) 420, 506
BstFNI CGCG 1 cut(s) 688
BstKTI GATC 1 cut(s) 568
BstMAI GTCTC 1 cut(s) 517
BstMBI GATC 1 cut(s) 565
BstNI CCWGG 1 cut(s) 348
BstNSI RCATGY 1 cut(s) 107
BstPI GGTNACC 1 cut(s) 488
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 2 cut(s) 612, 784
BstSLI GKGCMC 2 cut(s) 193, 800
BstUI CGCG 1 cut(s) 688
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 19
BstXI CCANNNNNNTGG 2 cut(s) 501, 662
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 2 cut(s) 44, 740
BtgI CCRYGG 2 cut(s) 398, 686
BtrI CACGTC 1 cut(s) 341
BtsCI GGATG 2 cut(s) 420, 506
BtsI GCAGTG 1 cut(s) 800
BtsIMutI CAGTG 4 cut(s) 356, 481, 793, 800
Cac8I GCNNGC 3 cut(s) 373, 690, 849
Cfr13I GGNCC 2 cut(s) 451, 739
Cfr42I CCGCGG 1 cut(s) 689
Csp6I GTAC 2 cut(s) 543, 635
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 6 cut(s) 104, 186, 399, 443, 524, 761
CviJI RGCY 9 cut(s) 8, 26, 44, 403, 522, 650, 740, 781, 847
CviKI_1 RGCY 9 cut(s) 8, 26, 44, 403, 522, 650, 740, 781, 847
CviQI GTAC 2 cut(s) 543, 635
DdeI CTNAG 1 cut(s) 270
DpnI GATC 1 cut(s) 567
DpnII GATC 1 cut(s) 565
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Ecl136II GAGCTC 1 cut(s) 781
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 783
Eco32I GATATC 2 cut(s) 208, 379
Eco47I GGWCC 1 cut(s) 451
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 1 cut(s) 648
Eco81I CCTNAGG 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 382
Eco91I GGTNACC 1 cut(s) 488
EcoICRI GAGCTC 1 cut(s) 781
EcoO65I GGTNACC 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 346
EcoRV GATATC 2 cut(s) 208, 379
EcoT14I CCWWGG 1 cut(s) 398
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 6 cut(s) 107, 189, 402, 446, 527, 764
FaqI GGGAC 1 cut(s) 380
FatI CATG 6 cut(s) 103, 185, 398, 442, 523, 760
FauI CCCGC 2 cut(s) 681, 693
FblI GTMKAC 1 cut(s) 875
Fnu4HI GCNGC 1 cut(s) 108
FokI GGATG 2 cut(s) 427, 513
FriOI GRGCYC 1 cut(s) 783
Fsp4HI GCNGC 1 cut(s) 108
FspBI CTAG 1 cut(s) 392
GluI GCNGC 1 cut(s) 108
HaeIII GGCC 2 cut(s) 44, 740
HapII CCGG 1 cut(s) 285
Hin1II CATG 6 cut(s) 107, 189, 402, 446, 527, 764
HinfI GANTC 3 cut(s) 293, 322, 510
HpaII CCGG 1 cut(s) 285
HphI GGTGA 4 cut(s) 179, 227, 500, 872
Hpy166II GTNNAC 1 cut(s) 876
Hpy188I TCNGA 6 cut(s) 22, 76, 99, 134, 298, 628
Hpy188III TCNNGA 3 cut(s) 384, 516, 702
Hpy8I GTNNAC 1 cut(s) 876
HpyAV CCTTC 2 cut(s) 206, 623
HpyCH4III ACNGT 3 cut(s) 360, 616, 788
HpyCH4IV ACGT 4 cut(s) 16, 340, 541, 735
HpyCH4V TGCA 6 cut(s) 308, 356, 481, 527, 643, 773
HpyF3I CTNAG 1 cut(s) 270
HpySE526I ACGT 4 cut(s) 16, 340, 541, 735
Hsp92II CATG 6 cut(s) 107, 189, 402, 446, 527, 764
KspI CCGCGG 1 cut(s) 689
Kzo9I GATC 1 cut(s) 565
LmnI GCTCC 1 cut(s) 5
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 2 cut(s) 284, 652
MaeI CTAG 1 cut(s) 392
MaeII ACGT 4 cut(s) 16, 340, 541, 735
MaeIII GTNAC 2 cut(s) 125, 488
MalI GATC 1 cut(s) 567
MboI GATC 1 cut(s) 565
MboII GAAGA 3 cut(s) 24, 101, 417
MhlI GDGCHC 3 cut(s) 193, 783, 800
MluCI AATT 2 cut(s) 472, 709
MlyI GAGTC 1 cut(s) 519
MmeI TCCRAC 2 cut(s) 54, 299
MnlI CCTC 9 cut(s) 34, 145, 196, 205, 442, 568, 692, 718, 810
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 3 cut(s) 16, 93, 292
MseI TTAA 4 cut(s) 117, 620, 708, 892
MslI CAYNNNNRTG 1 cut(s) 486
MspA1I CMGCKG 1 cut(s) 688
MspI CCGG 1 cut(s) 285
MspR9I CCNGG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 1 cut(s) 688
NcoI CCATGG 1 cut(s) 398
NdeII GATC 1 cut(s) 565
NlaIII CATG 6 cut(s) 107, 189, 402, 446, 527, 764
NlaIV GGNNCC 4 cut(s) 7, 62, 192, 741
NmuCI GTSAC 1 cut(s) 488
NsiI ATGCAT 1 cut(s) 310
NspI RCATGY 1 cut(s) 107
NspV TTCGAA 1 cut(s) 821
OliI CACNNNNGTG 1 cut(s) 486
PctI GAATGC 1 cut(s) 658
PdmI GAANNNNTTC 3 cut(s) 16, 93, 292
PfeI GAWTC 2 cut(s) 293, 322
PflMI CCANNNNNTGG 1 cut(s) 529
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 1 cut(s) 518
PpsI GAGTC 1 cut(s) 518
Psp124BI GAGCTC 1 cut(s) 783
Psp6I CCWGG 1 cut(s) 346
PspEI GGTNACC 1 cut(s) 488
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 4 cut(s) 7, 62, 192, 741
PspPI GGNCC 2 cut(s) 451, 739
RsaI GTAC 2 cut(s) 544, 636
RsaNI GTAC 2 cut(s) 543, 635
RseI CAYNNNNRTG 1 cut(s) 486
SacI GAGCTC 1 cut(s) 783
SacII CCGCGG 1 cut(s) 689
SaqAI TTAA 4 cut(s) 117, 620, 708, 892
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 1 cut(s) 565
Sau96I GGNCC 2 cut(s) 451, 739
SchI GAGTC 1 cut(s) 519
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 3 cut(s) 193, 783, 800
SfaNI GCATC 2 cut(s) 284, 652
SfcI CTRYAG 2 cut(s) 612, 784
Sfr303I CCGCGG 1 cut(s) 689
SfuI TTCGAA 1 cut(s) 821
SgrBI CCGCGG 1 cut(s) 689
SinI GGWCC 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 486
Sse9I AATT 2 cut(s) 472, 709
SsiI CCGC 3 cut(s) 460, 686, 688
SspMI CTAG 1 cut(s) 392
SstI GAGCTC 1 cut(s) 783
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 3 cut(s) 360, 616, 788
TaiI ACGT 4 cut(s) 19, 343, 544, 738
TaqI TCGA 4 cut(s) 210, 573, 646, 821
TasI AATT 2 cut(s) 472, 709
TfiI GAWTC 2 cut(s) 293, 322
Tru1I TTAA 4 cut(s) 117, 620, 708, 892
Tru9I TTAA 4 cut(s) 117, 620, 708, 892
TscAI CASTG 4 cut(s) 363, 488, 793, 800
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 3 cut(s) 417, 612, 746
TspRI CASTG 4 cut(s) 363, 488, 793, 800
Van91I CCANNNNNTGG 1 cut(s) 529
VpaK11BI GGWCC 1 cut(s) 451
XceI RCATGY 1 cut(s) 107
XmiI GTMKAC 1 cut(s) 875
XmnI GAANNNNTTC 3 cut(s) 16, 93, 292
XspI CTAG 1 cut(s) 392
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.