Rmu_sc0017459.1_g000002

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017459.1
OG0003891
Physical Location & Seq
Forward (+)
4139 .. 11964
7826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017459.1_g000002.1.cds

Sequence Viewer

Length: 3057 bp
atgcggacggcgaccggtgaccggaatccggcgaaagttggcagaattctggcagcatgtgacgggactccggcggccaatgacagagctccggcgaagtcttctatggcaatggaagggacgagttttgcagcagaatctcagccaaaagaggttcattcttctaacaacaatcagagaagtgataacacagttgagtgctctggcagtcctctaagtcgaaagatatcagctagatgggatccagatgaagcatgccggcctgtcattgatgatgcacctgtattttatccaactgccgaggagttcgaaaatacacttggttacatagcaaagatttggccacaggctgaatcttatggtatttgtcggattgtccctccacgttcctggatccctccttgccctcttaaagagaaagacatgtgggaaaatgctaaattctctactcgtgttcagcaagttgacttgcttcaaaatagagagtccatgaaaaagaaaactagaggtcggaagcgaaaacgaagaagacactcaaggagacgtgcagaagctaatgctgcttctgagaccgatgagaagtttgggttccagtcaggatcggactttacatttgcagagtttcagagacaagcctctactttcaaggaaagttactttggaacacatgaatgtaaggagggttcaaattctggtggaactaacaacaaaagatgggaaccttctgtggaggatattgaaggtgaatactggcgaatagtagagcaaccaacagacgaggttgaggtttactatggagctgatttggaaacaggagtatttggaagtgggtttcccaaggcatcgtctgtggtaactaggagtgagtcagatcattatgcaatgtcaggctggaatctaaataacttgcctcgcttgcctggttctgtactatgttttgaggaaagcgatatctcgggagttctagtcccatggctctatgttgggatgtgcttttcttcgttttgttggcatgttgaggaccaccacctctattccctaaattatctacacttcggtgacccaaaggtatggtatggagtgcctggaagccatgctacctctttggaacaagcaatgagaaagcatttgccggatttgtttgaggaacaacctgatttactcaatgaactggtcactcagctatctccttcagttttaaagtctgagggtgtacctgtgcatcgagctatccaacattctggggagtttgttcttacctttccgagggcatatcactctggatttaattgtggcttcaattgtgctgaggcagtaaatgtcgcccctgttgactggttgcaacatggccaaactgcagtcgagctttacagtaagcagtgtcgcaagacatcactgtcacacgacaaattgttactggggtcagctctggaagccgtgcaggtcctcggggaaatatcactcggaacaaaatttattaccaaccggagctggcaaaaagtttgtggcaaagatggtgtgctcaccaaagcagttaagatggaaaaagaatttgattcaaacactgagagagaatgcttttcttgctcctatgacttgcacttatctgctgccagctgcaactgctctcctgaccgattttcatgtcttaaacatgcaaagaatttctgttcatgtgaaatgacccagagatatgtccttctccgctacagtattgaggaattaaatttgctggttaaagcattggaaggggagttggatgccattcacgtatgggcatctaaggattctggagtagtttctatagactacactcgtaagtgcactgctaagaagcctaagctggatcaagattccaagtcttgcgatccaatggaaattttgcctggttgcgcagtttcagaagataaggttaacatgaatggatcttgcagttcctctagtcctgtttcttctgcagtagttcagtcaggatcacaggatggtcataatgaagatcaaactttggccgtgaatgctgaagcaaaggtggagcatgattgcagttttgatttgaatctcaattgtatgtctgatgaacatgaaagcaagataattgacgtatctgacggctgtgataataaggctagtacagttgaggaagagacatccacatcaatgtccaatcaagagaagactagcacgtccgagggtaataagttgtttggtgttgatcttggcttatcacgtcctgcttcaaacataccaccaatcagttcttcacaaactgagattggcgatacctcagctgtgaatgcatccatgggagaccaaagctatcgattaagatgttcgagtgttgttgagcctttgaatttcggatctgtcatggctgggaaatactggtgcaccaagccggccatatatccaaaagggtttagaagccgagttaatttctacagtgtgctggacccaacaaaactgtgtagttatatatcagaagttctcgatgctgggcttcttggacccctatttaaggttagtttagaagaattcccagatgaggcttttgcaaatgtatcagcggacaagtgctgggagatggtactacatagactaaacaatgaaataagtagacggagtagtctagacgaaggagggccccctttacagtctttgcagagcattaacgggtttgcaatgtttggctttctctcccaacctattgttgaggctattgaggcccttgatcctgatcatctatgcacagagtactggaatcacaggtgtacgcagcagcattcatctgtaccaagttgctctctaggagcgccgcagacaaagctatttggtatgaacatgacaaacaaggagcataatcaaccatctgtggagggtcatcactcaatcgatgagatgcagttagttttacgaagactcttagagaaggcgaatccaaatcccaaagagttgaggacattacatagaatgttatccagtcagtcagccgaagcaagagtggcatgcgcagaattgattgaagagatgcagagaaatttagataacagtaagtcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

1018

Amino Acids

113.12

Weight (kDa)

5.51

Isoelectric Point (pI)

54.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 1396, 2182, 2622
Acc16I TGCGCA 2 cut(s) 1894, 3007
Acc36I ACCTGC 1 cut(s) 1432
AccI GTMKAC 1 cut(s) 2614
AciI CCGC 5 cut(s) 4, 74, 1705, 2564, 2813
AcoI YGGCCR 5 cut(s) 75, 341, 1348, 2007, 2397
AcuI CTGAAG 2 cut(s) 1176, 2040
AfaI GTAC 7 cut(s) 930, 1215, 2131, 2586, 2753, 2770, 2790
AfiI CCNNNNNNNGG 5 cut(s) 21, 28, 1266, 2515, 2542
AflIII ACRYGT 1 cut(s) 423
AgeI ACCGGT 1 cut(s) 14
AjiI CACGTC 3 cut(s) 545, 2184, 2228
AjnI CCWGG 4 cut(s) 389, 919, 1084, 1885
AjuI GAANNNNNNNTTGG 6 cut(s) 303, 335, 642, 674, 2242, 2274
AleI CACNNNNGTG 1 cut(s) 1056
Alw21I GWGCWC 5 cut(s) 91, 203, 1524, 1826, 2390
Alw26I GTCTC 5 cut(s) 535, 563, 622, 2138, 2301
Alw44I GTGCAC 2 cut(s) 1822, 2386
Ama87I CYCGRG 2 cut(s) 955, 1448
AoxI GGCC 8 cut(s) 75, 260, 341, 1348, 2007, 2397, 2639, 2721
ApaI GGGCCC 1 cut(s) 2643
ApaLI GTGCAC 2 cut(s) 1822, 2386
ApeKI GCWGC 7 cut(s) 53, 131, 560, 1610, 1617, 2773, 2776
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AsiGI ACCGGT 1 cut(s) 14
AspLEI GCGC 3 cut(s) 1895, 2812, 3008
AspS9I GGNCC 7 cut(s) 1021, 1444, 2449, 2504, 2639, 2640, 2722
AsuHPI GGTGA 4 cut(s) 29, 755, 1070, 1516
AsuII TTCGAA 1 cut(s) 309
AvaI CYCGRG 2 cut(s) 955, 1448
AvaII GGWCC 4 cut(s) 1021, 1444, 2449, 2504
BaeGI GKGCMC 3 cut(s) 1826, 2390, 2643
BalI TGGCCA 2 cut(s) 343, 1350
BamHI GGATCC 2 cut(s) 241, 393
BanII GRGCYC 2 cut(s) 91, 2643
BarI GAAGNNNNNNTAC 2 cut(s) 1081, 1113
BauI CACGAG 1 cut(s) 450
BbsI GAAGAC 4 cut(s) 93, 535, 2180, 2920
Bbv12I GWGCWC 5 cut(s) 91, 203, 1524, 1826, 2390
BbvCI CCTCAGC 2 cut(s) 1308, 2284
BbvI GCAGC 7 cut(s) 65, 143, 547, 1597, 1604, 2785, 2788
BccI CCATC 7 cut(s) 231, 708, 1508, 1534, 1976, 2575, 2872
BceAI ACGGC 4 cut(s) 24, 1421, 1994, 2125
BciT130I CCWGG 4 cut(s) 391, 921, 1086, 1887
BclI TGATCA 1 cut(s) 2734
BcoDI GTCTC 5 cut(s) 535, 563, 622, 2138, 2301
BfaI CTAG 9 cut(s) 234, 504, 858, 965, 1941, 2127, 2178, 2627, 2804
BfmI CTRYAG 5 cut(s) 1356, 1708, 1803, 1956, 2437
BfoI RGCGCY 1 cut(s) 2813
BfuAI ACCTGC 1 cut(s) 1432
BisI GCNGC 9 cut(s) 54, 75, 132, 561, 1611, 1618, 2774, 2777, 2813
BlsI GCNGC 9 cut(s) 55, 76, 133, 562, 1612, 1619, 2775, 2778, 2814
BmcAI AGTACT 1 cut(s) 2753
Bme1390I CCNGG 4 cut(s) 391, 921, 1086, 1887
Bme18I GGWCC 4 cut(s) 1021, 1444, 2449, 2504
BmeT110I CYCGRG 2 cut(s) 955, 1448
BmgBI CACGTC 3 cut(s) 545, 2184, 2228
BmgT120I GGNCC 7 cut(s) 1021, 1444, 2449, 2504, 2639, 2640, 2722
BmiI GGNNCC 8 cut(s) 243, 395, 590, 720, 2451, 2506, 2641, 2642
BmrFI CCNGG 4 cut(s) 391, 921, 1086, 1887
BmrI ACTGGG 1 cut(s) 1427
BmsI GCATC 9 cut(s) 265, 851, 1231, 1750, 1787, 2306, 2479, 2886, 3015
BmuI ACTGGG 1 cut(s) 1427
BpiI GAAGAC 4 cut(s) 93, 535, 2180, 2920
BpmI CTGGAG 1 cut(s) 1812
Bpu10I CCTNAGC 3 cut(s) 1308, 1839, 2284
Bpu14I TTCGAA 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 520
Bsa29I ATCGAT 2 cut(s) 2320, 2889
BsaAI YACGTR 1 cut(s) 1771
BsaBI GATNNNNATC 2 cut(s) 2055, 2733
BsaI GGTCTC 2 cut(s) 563, 2301
BsaJI CCNNGG 7 cut(s) 300, 837, 971, 1265, 1447, 2187, 2301
BsaWI WCCGGW 3 cut(s) 14, 21, 1485
BsaXI ACNNNNNCTCC 2 cut(s) 951, 981
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 28, 1266, 2515, 2542
Bse118I RCCGGY 3 cut(s) 14, 258, 2395
Bse1I ACTGG 8 cut(s) 592, 755, 1176, 1340, 1422, 2387, 2759, 2976
Bse3DI GCAATG 4 cut(s) 117, 888, 1122, 2685
Bse8I GATNNNNATC 2 cut(s) 2055, 2733
BseBI CCWGG 4 cut(s) 391, 921, 1086, 1887
BseCI ATCGAT 2 cut(s) 2320, 2889
BseDI CCNNGG 7 cut(s) 300, 837, 971, 1265, 1447, 2187, 2301
BseGI GGATG 5 cut(s) 993, 1765, 1987, 2147, 2297
BseJI GATNNNNATC 2 cut(s) 2055, 2733
BseLI CCNNNNNNNGG 5 cut(s) 21, 28, 1266, 2515, 2542
BseMI GCAATG 4 cut(s) 117, 888, 1122, 2685
BseMII CTCAG 8 cut(s) 155, 558, 1193, 1197, 1299, 1557, 2259, 2298
BseNI ACTGG 8 cut(s) 592, 755, 1176, 1340, 1422, 2387, 2759, 2976
BseRI GAGGAG 1 cut(s) 317
BseSI GKGCMC 3 cut(s) 1826, 2390, 2643
BseXI GCAGC 7 cut(s) 65, 143, 547, 1597, 1604, 2785, 2788
BseYI CCCAGC 3 cut(s) 2372, 2492, 2574
BsgI GTGCAG 2 cut(s) 567, 1460
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 8 cut(s) 77, 262, 343, 1350, 2009, 2399, 2641, 2723
BshTI ACCGGT 1 cut(s) 14
BshVI ATCGAT 2 cut(s) 2320, 2889
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 5 cut(s) 91, 203, 1524, 1826, 2390
BsiHKCI CYCGRG 2 cut(s) 955, 1448
BsiSI CCGG 9 cut(s) 15, 22, 29, 71, 92, 259, 1133, 1486, 2396
BslFI GGGAC 4 cut(s) 79, 133, 362, 953
BslI CCNNNNNNNGG 5 cut(s) 21, 28, 1266, 2515, 2542
BsmAI GTCTC 5 cut(s) 535, 563, 622, 2138, 2301
BsmBI CGTCTC 1 cut(s) 535
BsmFI GGGAC 4 cut(s) 79, 133, 362, 953
BsmI GAATGC 4 cut(s) 1580, 2020, 2299, 2779
BsnI GGCC 8 cut(s) 77, 262, 343, 1350, 2009, 2399, 2641, 2723
Bso31I GGTCTC 2 cut(s) 563, 2301
BsoBI CYCGRG 2 cut(s) 955, 1448
Bsp119I TTCGAA 1 cut(s) 309
Bsp120I GGGCCC 1 cut(s) 2639
Bsp1286I GDGCHC 6 cut(s) 91, 203, 1524, 1826, 2390, 2643
Bsp19I CCATGG 2 cut(s) 971, 2301
BspACI CCGC 5 cut(s) 4, 74, 1705, 2564, 2813
BspANI GGCC 8 cut(s) 77, 262, 343, 1350, 2009, 2399, 2641, 2723
BspCNI CTCAG 8 cut(s) 154, 559, 1192, 1198, 1300, 1558, 2260, 2297
BspDI ATCGAT 2 cut(s) 2320, 2889
BspLI GGNNCC 8 cut(s) 243, 395, 590, 720, 2451, 2506, 2641, 2642
BspMAI CTGCAG 2 cut(s) 1360, 1960
BspMI ACCTGC 1 cut(s) 1432
BspT104I TTCGAA 1 cut(s) 309
BspTNI GGTCTC 2 cut(s) 563, 2301
BsrDI GCAATG 4 cut(s) 117, 888, 1122, 2685
BsrFI RCCGGY 3 cut(s) 14, 258, 2395
BsrI ACTGG 8 cut(s) 592, 755, 1176, 1340, 1422, 2387, 2759, 2976
BssAI RCCGGY 3 cut(s) 14, 258, 2395
BssECI CCNNGG 7 cut(s) 300, 837, 971, 1265, 1447, 2187, 2301
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 3 cut(s) 837, 971, 2301
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 4 cut(s) 391, 921, 1086, 1887
Bst4CI ACNGT 9 cut(s) 193, 1373, 1398, 1712, 2134, 2441, 2463, 2652, 3047
Bst6I CTCTTC 2 cut(s) 2136, 3015
BstBAI YACGTR 1 cut(s) 1771
BstBI TTCGAA 1 cut(s) 309
BstC8I GCNNGC 7 cut(s) 256, 260, 917, 1493, 1615, 2397, 3004
BstDSI CCRYGG 2 cut(s) 971, 2301
BstEII GGTNACC 2 cut(s) 17, 1058
BstENI CCTNNNNNAGG 2 cut(s) 1264, 2513
BstF5I GGATG 5 cut(s) 993, 1765, 1987, 2147, 2297
BstH2I RGCGCY 1 cut(s) 2813
BstHHI GCGC 3 cut(s) 1895, 2812, 3008
BstMAI GTCTC 5 cut(s) 535, 563, 622, 2138, 2301
BstMCI CGRYCG 1 cut(s) 15
BstNI CCWGG 4 cut(s) 391, 921, 1086, 1887
BstNSI RCATGY 6 cut(s) 60, 258, 427, 1016, 1658, 3006
BstPI GGTNACC 2 cut(s) 17, 1058
BstSCI CCNGG 4 cut(s) 389, 919, 1084, 1885
BstSFI CTRYAG 5 cut(s) 1356, 1708, 1803, 1956, 2437
BstSLI GKGCMC 3 cut(s) 1826, 2390, 2643
BstV1I GCAGC 7 cut(s) 65, 143, 547, 1597, 1604, 2785, 2788
BstV2I GAAGAC 4 cut(s) 93, 535, 2180, 2920
BstX2I RGATCY 4 cut(s) 241, 393, 1925, 2360
BstXI CCANNNNNNTGG 3 cut(s) 390, 1071, 1241
BstYI RGATCY 4 cut(s) 241, 393, 1925, 2360
Bsu15I ATCGAT 2 cut(s) 2320, 2889
BsuRI GGCC 8 cut(s) 77, 262, 343, 1350, 2009, 2399, 2641, 2723
BsuTUI ATCGAT 2 cut(s) 2320, 2889
BtgI CCRYGG 2 cut(s) 971, 2301
BtrI CACGTC 3 cut(s) 545, 2184, 2228
BtsCI GGATG 5 cut(s) 993, 1765, 1987, 2147, 2297
BtsI GCAGTG 2 cut(s) 1385, 1824
BtsIMutI CAGTG 5 cut(s) 1385, 1394, 1563, 1824, 2446
BveI ACCTGC 1 cut(s) 1432
Cac8I GCNNGC 7 cut(s) 256, 260, 917, 1493, 1615, 2397, 3004
CfoI GCGC 3 cut(s) 1895, 2812, 3008
Cfr10I RCCGGY 3 cut(s) 14, 258, 2395
Cfr13I GGNCC 7 cut(s) 1021, 1444, 2449, 2504, 2639, 2640, 2722
ClaI ATCGAT 2 cut(s) 2320, 2889
Csp6I GTAC 7 cut(s) 929, 1214, 2130, 2585, 2752, 2769, 2789
CspAI ACCGGT 1 cut(s) 14
CviQI GTAC 7 cut(s) 929, 1214, 2130, 2585, 2752, 2769, 2789
DraI TTTAAA 1 cut(s) 1200
DrdI GACNNNNNNGTC 3 cut(s) 1396, 2182, 2622
DseDI GACNNNNNNGTC 3 cut(s) 1396, 2182, 2622
EaeI YGGCCR 5 cut(s) 75, 341, 1348, 2007, 2397
Eam1104I CTCTTC 2 cut(s) 2136, 3015
EarI CTCTTC 2 cut(s) 2136, 3015
Ecl136II GAGCTC 1 cut(s) 89
Eco130I CCWWGG 3 cut(s) 837, 971, 2301
Eco24I GRGCYC 2 cut(s) 91, 2643
Eco31I GGTCTC 2 cut(s) 563, 2301
Eco32I GATATC 2 cut(s) 228, 952
Eco47I GGWCC 4 cut(s) 1021, 1444, 2449, 2504
Eco53kI GAGCTC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 1176, 2040
Eco88I CYCGRG 2 cut(s) 955, 1448
Eco91I GGTNACC 2 cut(s) 17, 1058
EcoICRI GAGCTC 1 cut(s) 89
EcoNI CCTNNNNNAGG 2 cut(s) 1264, 2513
EcoO109I RGGNCCY 4 cut(s) 1444, 2639, 2640, 2722
EcoO65I GGTNACC 2 cut(s) 17, 1058
EcoRI GAATTC 2 cut(s) 45, 2531
EcoRII CCWGG 4 cut(s) 389, 919, 1084, 1885
EcoRV GATATC 2 cut(s) 228, 952
EcoT14I CCWWGG 3 cut(s) 837, 971, 2301
EcoT22I ATGCAT 1 cut(s) 2299
EcoT38I GRGCYC 2 cut(s) 91, 2643
ErhI CCWWGG 3 cut(s) 837, 971, 2301
Esp3I CGTCTC 1 cut(s) 535
FaqI GGGAC 4 cut(s) 79, 133, 362, 953
FbaI TGATCA 1 cut(s) 2734
FblI GTMKAC 1 cut(s) 2614
Fnu4HI GCNGC 9 cut(s) 54, 75, 132, 561, 1611, 1618, 2774, 2777, 2813
FokI GGATG 5 cut(s) 1000, 1772, 1994, 2134, 2284
FriOI GRGCYC 2 cut(s) 91, 2643
Fsp4HI GCNGC 9 cut(s) 54, 75, 132, 561, 1611, 1618, 2774, 2777, 2813
FspBI CTAG 9 cut(s) 234, 504, 858, 965, 1941, 2127, 2178, 2627, 2804
FspI TGCGCA 2 cut(s) 1894, 3007
GlaI GCGC 3 cut(s) 1894, 2811, 3007
GluI GCNGC 9 cut(s) 54, 75, 132, 561, 1611, 1618, 2774, 2777, 2813
GsaI CCCAGC 3 cut(s) 2376, 2496, 2578
GsuI CTGGAG 1 cut(s) 1812
HaeII RGCGCY 1 cut(s) 2813
HaeIII GGCC 8 cut(s) 77, 262, 343, 1350, 2009, 2399, 2641, 2723
HapII CCGG 9 cut(s) 15, 22, 29, 71, 92, 259, 1133, 1486, 2396
HhaI GCGC 3 cut(s) 1895, 2812, 3008
Hin6I GCGC 3 cut(s) 1893, 2810, 3006
HinP1I GCGC 3 cut(s) 1893, 2810, 3006
HincII GTYRAC 3 cut(s) 466, 1333, 1915
HindII GTYRAC 3 cut(s) 466, 1333, 1915
HpaI GTTAAC 1 cut(s) 1915
HpaII CCGG 9 cut(s) 15, 22, 29, 71, 92, 259, 1133, 1486, 2396
HphI GGTGA 4 cut(s) 29, 755, 1070, 1516
Hpy166II GTNNAC 9 cut(s) 466, 790, 1214, 1333, 1824, 1915, 2388, 2615, 2769
Hpy8I GTNNAC 9 cut(s) 466, 790, 1214, 1333, 1824, 1915, 2388, 2615, 2769
HpyAV CCTTC 8 cut(s) 110, 732, 734, 1200, 1709, 1742, 2627, 2920
HpyCH4III ACNGT 9 cut(s) 193, 1373, 1398, 1712, 2134, 2441, 2463, 2652, 3047
HpyCH4IV ACGT 6 cut(s) 385, 544, 1770, 2100, 2183, 2227
HpySE526I ACGT 6 cut(s) 385, 544, 1770, 2100, 2183, 2227
HspAI GCGC 3 cut(s) 1893, 2810, 3006
KroI GCCGGC 2 cut(s) 258, 2395
KroNI GCCGGC 2 cut(s) 260, 2397
Ksp22I TGATCA 1 cut(s) 2734
KspAI GTTAAC 1 cut(s) 1915
LmnI GCTCC 7 cut(s) 94, 797, 1488, 1592, 2032, 2807, 2851
Lsp1109I GCAGC 7 cut(s) 65, 143, 547, 1597, 1604, 2785, 2788
LweI GCATC 9 cut(s) 265, 851, 1231, 1750, 1787, 2306, 2479, 2886, 3015
MaeI CTAG 9 cut(s) 234, 504, 858, 965, 1941, 2127, 2178, 2627, 2804
MaeII ACGT 6 cut(s) 385, 544, 1770, 2100, 2183, 2227
MaeIII GTNAC 9 cut(s) 17, 59, 323, 653, 853, 1058, 1174, 1398, 1413
MfeI CAATTG 2 cut(s) 1300, 2062
MflI RGATCY 4 cut(s) 241, 393, 1925, 2360
MhlI GDGCHC 6 cut(s) 91, 203, 1524, 1826, 2390, 2643
MlsI TGGCCA 2 cut(s) 343, 1350
MluNI TGGCCA 2 cut(s) 343, 1350
MlyI GAGTC 4 cut(s) 61, 494, 875, 2910
MmeI TCCRAC 5 cut(s) 317, 350, 491, 1258, 1737
Mox20I TGGCCA 2 cut(s) 343, 1350
Mph1103I ATGCAT 1 cut(s) 2299
MroNI GCCGGC 2 cut(s) 258, 2395
MscI TGGCCA 2 cut(s) 343, 1350
MslI CAYNNNNRTG 3 cut(s) 670, 1056, 2156
Msp20I TGGCCA 2 cut(s) 343, 1350
MspA1I CMGCKG 3 cut(s) 1617, 2288, 2564
MspI CCGG 9 cut(s) 15, 22, 29, 71, 92, 259, 1133, 1486, 2396
MspR9I CCNGG 4 cut(s) 391, 921, 1086, 1887
MunI CAATTG 2 cut(s) 1300, 2062
Mva1269I GAATGC 4 cut(s) 1580, 2020, 2299, 2779
MvaI CCWGG 4 cut(s) 391, 921, 1086, 1887
NaeI GCCGGC 2 cut(s) 260, 2397
NcoI CCATGG 2 cut(s) 971, 2301
NgoMIV GCCGGC 2 cut(s) 258, 2395
NlaIV GGNNCC 8 cut(s) 243, 395, 590, 720, 2451, 2506, 2641, 2642
NmeAIII GCCGAG 2 cut(s) 325, 2450
NmuCI GTSAC 5 cut(s) 17, 59, 1058, 1174, 1398
NsbI TGCGCA 2 cut(s) 1894, 3007
NsiI ATGCAT 1 cut(s) 2299
NspI RCATGY 6 cut(s) 60, 258, 427, 1016, 1658, 3006
NspV TTCGAA 1 cut(s) 309
OliI CACNNNNGTG 1 cut(s) 1056
PaeI GCATGC 2 cut(s) 258, 3006
PciI ACATGT 1 cut(s) 423
PctI GAATGC 4 cut(s) 1580, 2020, 2299, 2779
PdiI GCCGGC 2 cut(s) 260, 2397
PfoI TCCNGGA 1 cut(s) 389
PinAI ACCGGT 1 cut(s) 14
PkrI GCNGC 9 cut(s) 55, 76, 133, 562, 1612, 1619, 2775, 2778, 2814
PleI GAGTC 4 cut(s) 61, 493, 874, 2910
PpsI GAGTC 4 cut(s) 61, 493, 874, 2910
Ppu21I YACGTR 1 cut(s) 1771
PpuMI RGGWCCY 1 cut(s) 1444
PscI ACATGT 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 91
Psp5II RGGWCCY 1 cut(s) 1444
Psp6I CCWGG 4 cut(s) 389, 919, 1084, 1885
PspEI GGTNACC 2 cut(s) 17, 1058
PspFI CCCAGC 3 cut(s) 2372, 2492, 2574
PspGI CCWGG 4 cut(s) 389, 919, 1084, 1885
PspN4I GGNNCC 8 cut(s) 243, 395, 590, 720, 2451, 2506, 2641, 2642
PspOMI GGGCCC 1 cut(s) 2639
PspPI GGNCC 7 cut(s) 1021, 1444, 2449, 2504, 2639, 2640, 2722
PspPPI RGGWCCY 1 cut(s) 1444
PsrI GAACNNNNNNTAC 2 cut(s) 667, 699
PstI CTGCAG 2 cut(s) 1360, 1960
PsuI RGATCY 4 cut(s) 241, 393, 1925, 2360
PvuII CAGCTG 2 cut(s) 1617, 2288
RsaI GTAC 7 cut(s) 930, 1215, 2131, 2586, 2753, 2770, 2790
RsaNI GTAC 7 cut(s) 929, 1214, 2130, 2585, 2752, 2769, 2789
RseI CAYNNNNRTG 3 cut(s) 670, 1056, 2156
SacI GAGCTC 1 cut(s) 91
SatI GCNGC 9 cut(s) 54, 75, 132, 561, 1611, 1618, 2774, 2777, 2813
Sau96I GGNCC 7 cut(s) 1021, 1444, 2449, 2504, 2639, 2640, 2722
ScaI AGTACT 1 cut(s) 2753
SchI GAGTC 4 cut(s) 61, 494, 875, 2910
ScrFI CCNGG 4 cut(s) 391, 921, 1086, 1887
SduI GDGCHC 6 cut(s) 91, 203, 1524, 1826, 2390, 2643
SfaNI GCATC 9 cut(s) 265, 851, 1231, 1750, 1787, 2306, 2479, 2886, 3015
SfcI CTRYAG 5 cut(s) 1356, 1708, 1803, 1956, 2437
SfuI TTCGAA 1 cut(s) 309
SinI GGWCC 4 cut(s) 1021, 1444, 2449, 2504
SmiMI CAYNNNNRTG 3 cut(s) 670, 1056, 2156
SmlI CTYRAG 1 cut(s) 535
SmoI CTYRAG 1 cut(s) 535
SphI GCATGC 2 cut(s) 258, 3006
SsiI CCGC 5 cut(s) 4, 74, 1705, 2564, 2813
SspMI CTAG 9 cut(s) 234, 504, 858, 965, 1941, 2127, 2178, 2627, 2804
SstI GAGCTC 1 cut(s) 91
StyD4I CCNGG 4 cut(s) 389, 919, 1084, 1885
StyI CCWWGG 3 cut(s) 837, 971, 2301
TaaI ACNGT 9 cut(s) 193, 1373, 1398, 1712, 2134, 2441, 2463, 2652, 3047
TaiI ACGT 6 cut(s) 388, 547, 1773, 2103, 2186, 2230
TaqI TCGA 8 cut(s) 220, 309, 1225, 1362, 2320, 2333, 2487, 2889
TaqII GACCGA 2 cut(s) 587, 1650
TatI WGTACW 3 cut(s) 928, 2129, 2751
TauI GCSGC 2 cut(s) 77, 2815
TscAI CASTG 5 cut(s) 1385, 1401, 1570, 1831, 2446
TseFI GTSAC 5 cut(s) 17, 59, 1058, 1174, 1398
TseI GCWGC 7 cut(s) 53, 131, 560, 1610, 1617, 2773, 2776
Tsp45I GTSAC 5 cut(s) 17, 59, 1058, 1174, 1398
TspGWI ACGGA 1 cut(s) 2632
TspRI CASTG 5 cut(s) 1385, 1401, 1570, 1831, 2446
VneI GTGCAC 2 cut(s) 1822, 2386
VpaK11BI GGWCC 4 cut(s) 1021, 1444, 2449, 2504
XagI CCTNNNNNAGG 2 cut(s) 1264, 2513
XbaI TCTAGA 1 cut(s) 2626
XceI RCATGY 6 cut(s) 60, 258, 427, 1016, 1658, 3006
XmiI GTMKAC 1 cut(s) 2614
XspI CTAG 9 cut(s) 234, 504, 858, 965, 1941, 2127, 2178, 2627, 2804
ZrmI AGTACT 1 cut(s) 2753
Zsp2I ATGCAT 1 cut(s) 2299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.