RchiOBHm_Chr5g0018371

lysine-specific demethylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003891
Physical Location & Seq
Forward (+)
12942475 .. 12947832
5358 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29859

Sequence Viewer

Length: 3000 bp
ATGGAAGGGACGAGTTTTGCAGCAGAATCTCAGACAAAAGAGGTTCATTCTTCTAACAACAATCAGAGAAGTGATAACACAGTTGAGTGCTCTGGCAGTCCTCTAAGTCGAAAGATATCAGCTAGATGGGATCCAGATGAAGCATGCCGGCCTGTCATTGATGATGCACCTGTATTTTATCCAACTGCCGAGGAGTTCGAAAATACACTTGGTTACATAGCAGAGATTTGGCCACAGGCTGAATCTTATGGTATTTGTCGGATTGTCCCTCCACGTTCCTGGATCCCTCCTTGCCCTCTTAAAGAGAAAGACATGTGGGAAAATGCTAAATTCTCTACTCGTGTTCAGCAAGTTGACTTGCTTCAAAATAGAGAGTCCATGAAAAAGAAAACTAGAGGTCGGAAGCGAAAACGAAGAAGACACTCAAGGAGACGTGCAGAAGCTAATGCTGCTTCTGAGACCGATGAGAAGTTTGGGTTCCAGTCAGGATCAGACTTTACATTTGCAGAGTTTCAGAGACAAGCCTCTACTTTCAAGGAAAGTTACTTTGGAACACATGAATTTAAGGAGGGTTCAAATTCTGGTGGAACTAACAACAAAAGATGGGAACCCTCTGTGGAGGATATTGAAGGTGAATACTGGCGCATAGTAGAGCAACCAACAGATGAGGTTGAGGTTTACTATGGAGCTGACTTGGAAACAGGAGTGTTTGGAAGTGGGTTTCCCAAGGCATCGTCTGTGGTAACTAGGAGTGAGTCAGATCATTATGCAATGTCAGGCTGGAATCTAAATAACTTGCCTCGCTTGCCTGGTTCTGTACTATGTTTTGAGGAAAGCGATATCTCGGGAGTTCTAGTCCCATGGCTCTATGTTGGGATGTGCTTTTCTTCGTTTTGTTGGCATGTTGAGGACCACCACCTCTATTCCCTAAATTATCTACACTTCGGTGACCCAAAGGTATGGTATGGAGTGCCTGGAAGCCATGCTACCTCTTTGGAACAAGCAATGAGAAAGCATTTGCCGGATTTGTTTGAGGAACAACCTGATTTACTCAATGAACTGGTCACTCAGCTATCTCCTTCAGTTTTAAAGTCTGAGGGTGTACCTGTGCATCGAGCTATCCAACATTCTGGGGAGTTTGTTCTTACCTTTCCGAGGGCATATCATTCTGGATTTAATTGTGGCTTCAATTGTGCTGAGGCAGTAAATGTCGCCCCTGTTGACTGGTTGCAACATGGCCAAACTGCAGTTGAGCTTTACAGTAAGCAGTGCCGCAAGACGTCACTGTCACACGACAAATTGTTACTGGGGTCAGCTCTGGAAGCCGTGCAGGTCCTCGGGGAAATATCACTCGGAACAAAACTTATTACCAACCAGAGCTGGCAAAAAGTTTGTGGCAAAGATGGTGTGCTCACCAAAGCAGTTAAGAGACGAGTGGAGATGGAGGAGGAAAGACTGGATCGTCTTCCAATTTGTTGGAAGTCGCAAAAGATGGAAAGAGAATTTGATTCAAACACTGAGAGAGAATGCTTTTCTTGCTCCTATGACTTGCACTTATCTGCTGCCAGCTGCAACTGCTCTCCTGACCGATTTTCATGTCTTAAACATGCAAAGCATTTCTGTTCATGTGAAATGACTCAGAGATATGTCCTTCTCCGCTACAGTATTGAGGAATTAAATTTGCTGGTTAAAGCGTTGGAAGGGGAGTTGGATGCCATTCACGTATGGGCATCTAAGGATTCTGGAGTAGTTTCTATAGACTACACTCGTAAGTGCGCTGCTAAGAAGCCTAAGCTGGATCAAGATTCCAAGTCTTGCGATCCAATGGAAATTTTGCCAGTTTCACAAGATAAGGTTAACATGAATGGATCTTGCAGTTCCTCTAGTCCTGTTTCTTCTGCAGTAGTTCAGTCAGGATCACAGGATGGTCATAATGAAGATCAAACTTTGGCCGTGAATGCTGAGGCAAAGGTGGAGCATGATTGCAGTTTTGATTTGAATCTCAATTGTATGTCTGATGAACATGAAAGCAAGATAATTGACGTATCTGACGGCTGTGATAATAAGGCTAGTACAGTTGAGGAAGAGACATCCACATCAATGTCCAATCAAGAGAAGACTAGCACGTCTGAGGCTAATAAGTTGTTTGGTGTTGATCTTGGCTTATCACGTCCTGCTTCAAACATACCACCAATCAGTTCTTCACAAACTGAGATTGGCGATACCTCAGCTGTGAATGCATCCATGAGAGACCAAAGCTATCGATTAGGATGTTTGAGTGTTGTTGAGCCTTTGAATTTCGGAGCTGTCATGGCTGGGAAATATTGGTGCACTAAGCAGGCCATATATCCAAAAGGGTTTAGAAGCCGAGTTAATTTCTACAGTGTGCTGGACCCAACAAAACTGTGTAGTTATATATCAGAAGTTCTCGATGCTGGGCTTCTTGGACCCCTATTTAAGGTTAGTTTAGAAGAATTCCCAGATGAGGCTTTTGCAAATGTATCAGCAGACAAGTGCTGGGAGATGGTACTACATAGACTAAACAATGAAATAAGTAGACGGAGTAGTCTAGACGAAAGAGGGCCCCCTTTACAGTCTTTGCAGAGCATTAACGGGTTTGCAATGTTTGGCTTTCTCTCCCAACCTATTGTTGAGGCTATTGAGGCCCTTGATCCTGATCATCTATGCACAGAGTACTGGAATCACAGGTGTACGCAGCAGCATTCATCTGTACCAAGTTGCTCTCTAGGAGCGCCGCAGACAAAGCTATTTGGTATGAACATGACAAACAAGGAGCATAATCAACCATCTGTGGAGGGTCATCACTCAATCGATGAGATGCAGTTAGTTTTACGAAGACTCTTAGAGAAGGCGAATCCAAATCCCGAAGAGTTAAGGACATTACATAGAATGTTATCCAGTCAGTCAGCCGAAGCAAGAGTGGCATGCGCAGAATTGATTGAAGAGATGCAGAGAAATGTAGATAACAGGAAGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006482 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008213 GO:0008214 GO:0008276 GO:0009314 GO:0009416 GO:0009555 GO:0009628 GO:0009648 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009910 GO:0009987 GO:0010154 GO:0010216 GO:0010228 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016569 GO:0016570 GO:0016571 GO:0016577 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0019538 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0032451 GO:0032452 GO:0032453 GO:0032501 GO:0032502 GO:0034641 GO:0034647 GO:0034720 GO:0034721 GO:0036211 GO:0042054 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048316 GO:0048519 GO:0048523 GO:0048573 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140096 GO:0140110 GO:1901360 GO:1901363 GO:1901564 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000026 GO:2000028 GO:2000112 GO:2000113 GO:2000241 GO:2000242 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FYRC FYRN JmjC JmjN zf-C5HC2

Protein Analysis

999

Amino Acids

112.07

Weight (kDa)

5.42

Isoelectric Point (pI)

55.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JmjN PF02375 57 - 90 8.4e-15 jmjN domain
JmjC PF02373 288 - 404 2.6e-46 JmjC domain, hydroxylase
zf-C5HC2 PF02928 510 - 560 1.8e-14 C5HC2 zinc finger
FYRN PF05964 765 - 811 1e-11 F/Y-rich N-terminus
FYRC PF05965 816 - 903 6.2e-21 F/Y rich C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 4 cut(s) 1285, 1461, 2125, 2565
AatII GACGTC 1 cut(s) 1283
Acc16I TGCGCA 1 cut(s) 2950
Acc36I ACCTGC 1 cut(s) 1321
AccI GTMKAC 1 cut(s) 2557
AciI CCGC 3 cut(s) 1273, 1657, 2756
AcoI YGGCCR 3 cut(s) 230, 1237, 1950
AcsI RAATTY 8 cut(s) 329, 560, 577, 1502, 1678, 1830, 2296, 2474
AcuI CTGAAG 1 cut(s) 1065
AcyI GRCGYC 1 cut(s) 1280
AfaI GTAC 7 cut(s) 819, 1104, 2074, 2529, 2696, 2713, 2733
AfiI CCNNNNNNNGG 3 cut(s) 1155, 2458, 2485
AflIII ACRYGT 1 cut(s) 312
AjiI CACGTC 3 cut(s) 434, 2127, 2171
AjnI CCWGG 3 cut(s) 278, 808, 973
AjuI GAANNNNNNNTTGG 6 cut(s) 192, 224, 531, 563, 2185, 2217
AleI CACNNNNGTG 1 cut(s) 945
Alw21I GWGCWC 3 cut(s) 92, 1413, 2333
Alw26I GTCTC 6 cut(s) 424, 452, 511, 1423, 2081, 2244
Alw44I GTGCAC 1 cut(s) 2329
Ama87I CYCGRG 2 cut(s) 844, 1337
AoxI GGCC 7 cut(s) 149, 230, 1237, 1950, 2340, 2582, 2664
ApaI GGGCCC 1 cut(s) 2586
ApaLI GTGCAC 1 cut(s) 2329
ApeKI GCWGC 7 cut(s) 20, 449, 1562, 1569, 1778, 2716, 2719
ApoI RAATTY 8 cut(s) 329, 560, 577, 1502, 1678, 1830, 2296, 2474
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspLEI GCGC 4 cut(s) 645, 1778, 2755, 2951
AspS9I GGNCC 7 cut(s) 910, 1333, 2392, 2447, 2582, 2583, 2665
AsuHPI GGTGA 3 cut(s) 644, 959, 1405
AsuII TTCGAA 1 cut(s) 198
AvaI CYCGRG 2 cut(s) 844, 1337
AvaII GGWCC 4 cut(s) 910, 1333, 2392, 2447
BaeGI GKGCMC 2 cut(s) 2333, 2586
BalI TGGCCA 2 cut(s) 232, 1239
BamHI GGATCC 2 cut(s) 130, 282
BanII GRGCYC 1 cut(s) 2586
BarI GAAGNNNNNNTAC 2 cut(s) 970, 1002
BauI CACGAG 1 cut(s) 339
BbsI GAAGAC 4 cut(s) 424, 1457, 2123, 2863
Bbv12I GWGCWC 3 cut(s) 92, 1413, 2333
BbvCI CCTCAGC 3 cut(s) 1197, 1962, 2227
BbvI GCAGC 7 cut(s) 32, 436, 1549, 1556, 1765, 2728, 2731
BccI CCATC 8 cut(s) 120, 597, 1397, 1435, 1486, 1919, 2518, 2815
BceAI ACGGC 3 cut(s) 1310, 1937, 2068
BciT130I CCWGG 3 cut(s) 280, 810, 975
BclI TGATCA 1 cut(s) 2677
BcoDI GTCTC 6 cut(s) 424, 452, 511, 1423, 2081, 2244
BfaI CTAG 9 cut(s) 123, 393, 747, 854, 1884, 2070, 2121, 2570, 2747
BfmI CTRYAG 5 cut(s) 1245, 1660, 1755, 1899, 2380
BfoI RGCGCY 1 cut(s) 2756
BfuAI ACCTGC 1 cut(s) 1321
BisI GCNGC 9 cut(s) 21, 450, 1273, 1563, 1570, 1779, 2717, 2720, 2756
BlsI GCNGC 9 cut(s) 22, 451, 1274, 1564, 1571, 1780, 2718, 2721, 2757
BmcAI AGTACT 1 cut(s) 2696
Bme1390I CCNGG 3 cut(s) 280, 810, 975
Bme18I GGWCC 4 cut(s) 910, 1333, 2392, 2447
BmeT110I CYCGRG 2 cut(s) 844, 1337
BmgBI CACGTC 3 cut(s) 434, 2127, 2171
BmgT120I GGNCC 7 cut(s) 910, 1333, 2392, 2447, 2582, 2583, 2665
BmiI GGNNCC 8 cut(s) 132, 284, 479, 609, 2394, 2449, 2584, 2585
BmrFI CCNGG 3 cut(s) 280, 810, 975
BmrI ACTGGG 1 cut(s) 1316
BmsI GCATC 9 cut(s) 154, 740, 1120, 1702, 1739, 2249, 2422, 2829, 2958
BmuI ACTGGG 1 cut(s) 1316
BpiI GAAGAC 4 cut(s) 424, 1457, 2123, 2863
BpmI CTGGAG 1 cut(s) 1764
Bpu10I CCTNAGC 4 cut(s) 1197, 1791, 1962, 2227
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 409
Bsa29I ATCGAT 2 cut(s) 2263, 2832
BsaAI YACGTR 1 cut(s) 1723
BsaBI GATNNNNATC 2 cut(s) 1998, 2676
BsaHI GRCGYC 1 cut(s) 1280
BsaI GGTCTC 2 cut(s) 452, 2244
BsaJI CCNNGG 5 cut(s) 189, 726, 860, 1154, 1336
BsaXI ACNNNNNCTCC 2 cut(s) 840, 870
Bsc4I CCNNNNNNNGG 3 cut(s) 1155, 2458, 2485
Bse118I RCCGGY 1 cut(s) 147
Bse1I ACTGG 9 cut(s) 481, 644, 1065, 1229, 1311, 1461, 1838, 2702, 2919
Bse3DI GCAATG 3 cut(s) 777, 1011, 2628
Bse8I GATNNNNATC 2 cut(s) 1998, 2676
BseBI CCWGG 3 cut(s) 280, 810, 975
BseCI ATCGAT 2 cut(s) 2263, 2832
BseDI CCNNGG 5 cut(s) 189, 726, 860, 1154, 1336
BseGI GGATG 6 cut(s) 882, 1717, 1930, 2090, 2240, 2276
BseJI GATNNNNATC 2 cut(s) 1998, 2676
BseLI CCNNNNNNNGG 3 cut(s) 1155, 2458, 2485
BseMI GCAATG 3 cut(s) 777, 1011, 2628
BseNI ACTGG 9 cut(s) 481, 644, 1065, 1229, 1311, 1461, 1838, 2702, 2919
BseRI GAGGAG 2 cut(s) 206, 1460
BseSI GKGCMC 2 cut(s) 2333, 2586
BseXI GCAGC 7 cut(s) 32, 436, 1549, 1556, 1765, 2728, 2731
BseYI CCCAGC 3 cut(s) 2315, 2435, 2517
BsgI GTGCAG 2 cut(s) 456, 1349
BshFI GGCC 7 cut(s) 151, 232, 1239, 1952, 2342, 2584, 2666
BshVI ATCGAT 2 cut(s) 2263, 2832
BsiHKAI GWGCWC 3 cut(s) 92, 1413, 2333
BsiHKCI CYCGRG 2 cut(s) 844, 1337
BsiSI CCGG 2 cut(s) 148, 1022
BslFI GGGAC 3 cut(s) 22, 251, 842
BslI CCNNNNNNNGG 3 cut(s) 1155, 2458, 2485
BsmAI GTCTC 6 cut(s) 424, 452, 511, 1423, 2081, 2244
BsmBI CGTCTC 2 cut(s) 424, 1423
BsmFI GGGAC 3 cut(s) 22, 251, 842
BsmI GAATGC 4 cut(s) 1532, 1963, 2242, 2722
BsnI GGCC 7 cut(s) 151, 232, 1239, 1952, 2342, 2584, 2666
Bso31I GGTCTC 2 cut(s) 452, 2244
BsoBI CYCGRG 2 cut(s) 844, 1337
Bsp119I TTCGAA 1 cut(s) 198
Bsp120I GGGCCC 1 cut(s) 2582
Bsp1286I GDGCHC 4 cut(s) 92, 1413, 2333, 2586
Bsp19I CCATGG 1 cut(s) 860
BspACI CCGC 3 cut(s) 1273, 1657, 2756
BspANI GGCC 7 cut(s) 151, 232, 1239, 1952, 2342, 2584, 2666
BspDI ATCGAT 2 cut(s) 2263, 2832
BspLI GGNNCC 8 cut(s) 132, 284, 479, 609, 2394, 2449, 2584, 2585
BspMAI CTGCAG 2 cut(s) 1249, 1903
BspMI ACCTGC 1 cut(s) 1321
BspT104I TTCGAA 1 cut(s) 198
BspTNI GGTCTC 2 cut(s) 452, 2244
BsrDI GCAATG 3 cut(s) 777, 1011, 2628
BsrFI RCCGGY 1 cut(s) 147
BsrI ACTGG 9 cut(s) 481, 644, 1065, 1229, 1311, 1461, 1838, 2702, 2919
BssAI RCCGGY 1 cut(s) 147
BssECI CCNNGG 5 cut(s) 189, 726, 860, 1154, 1336
BssNI GRCGYC 1 cut(s) 1280
BssSI CACGAG 1 cut(s) 339
BssT1I CCWWGG 2 cut(s) 726, 860
Bst2BI CACGAG 1 cut(s) 339
Bst2UI CCWGG 3 cut(s) 280, 810, 975
Bst4CI ACNGT 8 cut(s) 82, 1262, 1287, 1664, 2077, 2384, 2406, 2595
Bst6I CTCTTC 3 cut(s) 2079, 2883, 2958
BstACI GRCGYC 1 cut(s) 1280
BstBAI YACGTR 1 cut(s) 1723
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 7 cut(s) 145, 149, 806, 1382, 1567, 2340, 2947
BstDSI CCRYGG 1 cut(s) 860
BstEII GGTNACC 1 cut(s) 947
BstENI CCTNNNNNAGG 2 cut(s) 1153, 2456
BstF5I GGATG 6 cut(s) 882, 1717, 1930, 2090, 2240, 2276
BstH2I RGCGCY 1 cut(s) 2756
BstHHI GCGC 4 cut(s) 645, 1778, 2755, 2951
BstMAI GTCTC 6 cut(s) 424, 452, 511, 1423, 2081, 2244
BstNI CCWGG 3 cut(s) 280, 810, 975
BstNSI RCATGY 5 cut(s) 147, 316, 905, 1610, 2949
BstPI GGTNACC 1 cut(s) 947
BstSCI CCNGG 3 cut(s) 278, 808, 973
BstSFI CTRYAG 5 cut(s) 1245, 1660, 1755, 1899, 2380
BstSLI GKGCMC 2 cut(s) 2333, 2586
BstV1I GCAGC 7 cut(s) 32, 436, 1549, 1556, 1765, 2728, 2731
BstV2I GAAGAC 4 cut(s) 424, 1457, 2123, 2863
BstX2I RGATCY 3 cut(s) 130, 282, 1868
BstXI CCANNNNNNTGG 4 cut(s) 279, 960, 1130, 1476
BstYI RGATCY 3 cut(s) 130, 282, 1868
Bsu15I ATCGAT 2 cut(s) 2263, 2832
BsuRI GGCC 7 cut(s) 151, 232, 1239, 1952, 2342, 2584, 2666
BsuTUI ATCGAT 2 cut(s) 2263, 2832
BtgI CCRYGG 1 cut(s) 860
BtrI CACGTC 3 cut(s) 434, 2127, 2171
BtsCI GGATG 6 cut(s) 882, 1717, 1930, 2090, 2240, 2276
BtsI GCAGTG 1 cut(s) 1274
BtsIMutI CAGTG 4 cut(s) 1274, 1283, 1515, 2389
BveI ACCTGC 1 cut(s) 1321
Cac8I GCNNGC 7 cut(s) 145, 149, 806, 1382, 1567, 2340, 2947
CfoI GCGC 4 cut(s) 645, 1778, 2755, 2951
Cfr10I RCCGGY 1 cut(s) 147
Cfr13I GGNCC 7 cut(s) 910, 1333, 2392, 2447, 2582, 2583, 2665
ClaI ATCGAT 2 cut(s) 2263, 2832
Csp6I GTAC 7 cut(s) 818, 1103, 2073, 2528, 2695, 2712, 2732
CviQI GTAC 7 cut(s) 818, 1103, 2073, 2528, 2695, 2712, 2732
DraI TTTAAA 1 cut(s) 1089
DrdI GACNNNNNNGTC 4 cut(s) 1285, 1461, 2125, 2565
DseDI GACNNNNNNGTC 4 cut(s) 1285, 1461, 2125, 2565
EaeI YGGCCR 3 cut(s) 230, 1237, 1950
Eam1104I CTCTTC 3 cut(s) 2079, 2883, 2958
EarI CTCTTC 3 cut(s) 2079, 2883, 2958
Eco130I CCWWGG 2 cut(s) 726, 860
Eco24I GRGCYC 1 cut(s) 2586
Eco31I GGTCTC 2 cut(s) 452, 2244
Eco32I GATATC 2 cut(s) 117, 841
Eco47I GGWCC 4 cut(s) 910, 1333, 2392, 2447
Eco57I CTGAAG 1 cut(s) 1065
Eco88I CYCGRG 2 cut(s) 844, 1337
Eco91I GGTNACC 1 cut(s) 947
EcoNI CCTNNNNNAGG 2 cut(s) 1153, 2456
EcoO109I RGGNCCY 4 cut(s) 1333, 2582, 2583, 2665
EcoO65I GGTNACC 1 cut(s) 947
EcoRI GAATTC 1 cut(s) 2474
EcoRII CCWGG 3 cut(s) 278, 808, 973
EcoRV GATATC 2 cut(s) 117, 841
EcoT14I CCWWGG 2 cut(s) 726, 860
EcoT22I ATGCAT 1 cut(s) 2242
EcoT38I GRGCYC 1 cut(s) 2586
ErhI CCWWGG 2 cut(s) 726, 860
Esp3I CGTCTC 2 cut(s) 424, 1423
FaqI GGGAC 3 cut(s) 22, 251, 842
FbaI TGATCA 1 cut(s) 2677
FblI GTMKAC 1 cut(s) 2557
Fnu4HI GCNGC 9 cut(s) 21, 450, 1273, 1563, 1570, 1779, 2717, 2720, 2756
FokI GGATG 6 cut(s) 889, 1724, 1937, 2077, 2227, 2283
FriOI GRGCYC 1 cut(s) 2586
Fsp4HI GCNGC 9 cut(s) 21, 450, 1273, 1563, 1570, 1779, 2717, 2720, 2756
FspBI CTAG 9 cut(s) 123, 393, 747, 854, 1884, 2070, 2121, 2570, 2747
FspI TGCGCA 1 cut(s) 2950
GlaI GCGC 4 cut(s) 644, 1777, 2754, 2950
GluI GCNGC 9 cut(s) 21, 450, 1273, 1563, 1570, 1779, 2717, 2720, 2756
GsaI CCCAGC 3 cut(s) 2319, 2439, 2521
GsuI CTGGAG 1 cut(s) 1764
HaeII RGCGCY 1 cut(s) 2756
HaeIII GGCC 7 cut(s) 151, 232, 1239, 1952, 2342, 2584, 2666
HapII CCGG 2 cut(s) 148, 1022
HhaI GCGC 4 cut(s) 645, 1778, 2755, 2951
Hin1I GRCGYC 1 cut(s) 1280
Hin6I GCGC 4 cut(s) 643, 1776, 2753, 2949
HinP1I GCGC 4 cut(s) 643, 1776, 2753, 2949
HincII GTYRAC 3 cut(s) 355, 1222, 1858
HindII GTYRAC 3 cut(s) 355, 1222, 1858
HpaI GTTAAC 1 cut(s) 1858
HpaII CCGG 2 cut(s) 148, 1022
HphI GGTGA 3 cut(s) 644, 959, 1405
Hpy166II GTNNAC 8 cut(s) 355, 679, 1103, 1222, 1858, 2331, 2558, 2712
Hpy8I GTNNAC 8 cut(s) 355, 679, 1103, 1222, 1858, 2331, 2558, 2712
HpyAV CCTTC 5 cut(s) 623, 1089, 1661, 1694, 2863
HpyCH4III ACNGT 8 cut(s) 82, 1262, 1287, 1664, 2077, 2384, 2406, 2595
HpyCH4IV ACGT 7 cut(s) 274, 433, 1280, 1722, 2043, 2126, 2170
HpySE526I ACGT 7 cut(s) 274, 433, 1280, 1722, 2043, 2126, 2170
Hsp92I GRCGYC 1 cut(s) 1280
HspAI GCGC 4 cut(s) 643, 1776, 2753, 2949
KroI GCCGGC 1 cut(s) 147
KroNI GCCGGC 1 cut(s) 149
Ksp22I TGATCA 1 cut(s) 2677
KspAI GTTAAC 1 cut(s) 1858
LmnI GCTCC 6 cut(s) 686, 1544, 1975, 2303, 2750, 2794
Lsp1109I GCAGC 7 cut(s) 32, 436, 1549, 1556, 1765, 2728, 2731
LweI GCATC 9 cut(s) 154, 740, 1120, 1702, 1739, 2249, 2422, 2829, 2958
MaeI CTAG 9 cut(s) 123, 393, 747, 854, 1884, 2070, 2121, 2570, 2747
MaeII ACGT 7 cut(s) 274, 433, 1280, 1722, 2043, 2126, 2170
MaeIII GTNAC 8 cut(s) 212, 542, 742, 947, 1063, 1281, 1287, 1302
MfeI CAATTG 2 cut(s) 1189, 2005
MflI RGATCY 3 cut(s) 130, 282, 1868
MhlI GDGCHC 4 cut(s) 92, 1413, 2333, 2586
MlsI TGGCCA 2 cut(s) 232, 1239
MluNI TGGCCA 2 cut(s) 232, 1239
MlyI GAGTC 4 cut(s) 383, 764, 1630, 2853
MmeI TCCRAC 7 cut(s) 206, 239, 380, 1147, 1457, 1677, 1689
Mox20I TGGCCA 2 cut(s) 232, 1239
Mph1103I ATGCAT 1 cut(s) 2242
MroNI GCCGGC 1 cut(s) 147
MscI TGGCCA 2 cut(s) 232, 1239
MslI CAYNNNNRTG 2 cut(s) 945, 2099
Msp20I TGGCCA 2 cut(s) 232, 1239
MspA1I CMGCKG 2 cut(s) 1569, 2231
MspI CCGG 2 cut(s) 148, 1022
MspR9I CCNGG 3 cut(s) 280, 810, 975
MunI CAATTG 2 cut(s) 1189, 2005
Mva1269I GAATGC 4 cut(s) 1532, 1963, 2242, 2722
MvaI CCWGG 3 cut(s) 280, 810, 975
NaeI GCCGGC 1 cut(s) 149
NcoI CCATGG 1 cut(s) 860
NgoMIV GCCGGC 1 cut(s) 147
NlaIV GGNNCC 8 cut(s) 132, 284, 479, 609, 2394, 2449, 2584, 2585
NmeAIII GCCGAG 2 cut(s) 214, 2393
NmuCI GTSAC 4 cut(s) 947, 1063, 1281, 1287
NsbI TGCGCA 1 cut(s) 2950
NsiI ATGCAT 1 cut(s) 2242
NspI RCATGY 5 cut(s) 147, 316, 905, 1610, 2949
NspV TTCGAA 1 cut(s) 198
OliI CACNNNNGTG 1 cut(s) 945
PaeI GCATGC 2 cut(s) 147, 2949
PciI ACATGT 1 cut(s) 312
PctI GAATGC 4 cut(s) 1532, 1963, 2242, 2722
PdiI GCCGGC 1 cut(s) 149
PfeI GAWTC 9 cut(s) 26, 242, 784, 1508, 1739, 1805, 1999, 2701, 2875
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 9 cut(s) 22, 451, 1274, 1564, 1571, 1780, 2718, 2721, 2757
PleI GAGTC 4 cut(s) 382, 763, 1630, 2853
PpsI GAGTC 4 cut(s) 382, 763, 1630, 2853
Ppu21I YACGTR 1 cut(s) 1723
PpuMI RGGWCCY 1 cut(s) 1333
PscI ACATGT 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 1333
Psp6I CCWGG 3 cut(s) 278, 808, 973
PspEI GGTNACC 1 cut(s) 947
PspFI CCCAGC 3 cut(s) 2315, 2435, 2517
PspGI CCWGG 3 cut(s) 278, 808, 973
PspN4I GGNNCC 8 cut(s) 132, 284, 479, 609, 2394, 2449, 2584, 2585
PspOMI GGGCCC 1 cut(s) 2582
PspPI GGNCC 7 cut(s) 910, 1333, 2392, 2447, 2582, 2583, 2665
PspPPI RGGWCCY 1 cut(s) 1333
PstI CTGCAG 2 cut(s) 1249, 1903
PsuI RGATCY 3 cut(s) 130, 282, 1868
PvuII CAGCTG 2 cut(s) 1569, 2231
RsaI GTAC 7 cut(s) 819, 1104, 2074, 2529, 2696, 2713, 2733
RsaNI GTAC 7 cut(s) 818, 1103, 2073, 2528, 2695, 2712, 2732
RseI CAYNNNNRTG 2 cut(s) 945, 2099
SatI GCNGC 9 cut(s) 21, 450, 1273, 1563, 1570, 1779, 2717, 2720, 2756
Sau96I GGNCC 7 cut(s) 910, 1333, 2392, 2447, 2582, 2583, 2665
ScaI AGTACT 1 cut(s) 2696
SchI GAGTC 4 cut(s) 383, 764, 1630, 2853
ScrFI CCNGG 3 cut(s) 280, 810, 975
SduI GDGCHC 4 cut(s) 92, 1413, 2333, 2586
SfaNI GCATC 9 cut(s) 154, 740, 1120, 1702, 1739, 2249, 2422, 2829, 2958
SfcI CTRYAG 5 cut(s) 1245, 1660, 1755, 1899, 2380
SfuI TTCGAA 1 cut(s) 198
SinI GGWCC 4 cut(s) 910, 1333, 2392, 2447
SmiMI CAYNNNNRTG 2 cut(s) 945, 2099
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SphI GCATGC 2 cut(s) 147, 2949
SsiI CCGC 3 cut(s) 1273, 1657, 2756
SspI AATATT 1 cut(s) 2324
SspMI CTAG 9 cut(s) 123, 393, 747, 854, 1884, 2070, 2121, 2570, 2747
StyD4I CCNGG 3 cut(s) 278, 808, 973
StyI CCWWGG 2 cut(s) 726, 860
TaaI ACNGT 8 cut(s) 82, 1262, 1287, 1664, 2077, 2384, 2406, 2595
TaiI ACGT 7 cut(s) 277, 436, 1283, 1725, 2046, 2129, 2173
TaqI TCGA 6 cut(s) 109, 198, 1114, 2263, 2430, 2832
TaqII GACCGA 2 cut(s) 476, 1602
TatI WGTACW 3 cut(s) 817, 2072, 2694
TauI GCSGC 2 cut(s) 1275, 2758
TfiI GAWTC 9 cut(s) 26, 242, 784, 1508, 1739, 1805, 1999, 2701, 2875
TscAI CASTG 4 cut(s) 1274, 1290, 1522, 2389
TseFI GTSAC 4 cut(s) 947, 1063, 1281, 1287
TseI GCWGC 7 cut(s) 20, 449, 1562, 1569, 1778, 2716, 2719
Tsp45I GTSAC 4 cut(s) 947, 1063, 1281, 1287
TspGWI ACGGA 1 cut(s) 2575
TspRI CASTG 4 cut(s) 1274, 1290, 1522, 2389
VneI GTGCAC 1 cut(s) 2329
VpaK11BI GGWCC 4 cut(s) 910, 1333, 2392, 2447
XagI CCTNNNNNAGG 2 cut(s) 1153, 2456
XapI RAATTY 8 cut(s) 329, 560, 577, 1502, 1678, 1830, 2296, 2474
XbaI TCTAGA 1 cut(s) 2569
XceI RCATGY 5 cut(s) 147, 316, 905, 1610, 2949
XmiI GTMKAC 1 cut(s) 2557
XspI CTAG 9 cut(s) 123, 393, 747, 854, 1884, 2070, 2121, 2570, 2747
ZraI GACGTC 1 cut(s) 1281
ZrmI AGTACT 1 cut(s) 2696
Zsp2I ATGCAT 1 cut(s) 2242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.