Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003116

Physical Location & Seq

Genomic Coordinates Forward (+)

34793742 .. 34796130

Gene Structure 2389 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1157200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGGGAGACCAAAGTGCAAGAGGAAAATTGAATCTCTTTCTTCACAAGATAAAGCCTTACGTGGCTATGGTTTCTTTGCAATTTGGATATGCAGGAATGTATATTCTCAGCATGGTTGGTTTGAGGCGTGGCATCAATCACTTTGTTCTTTCTGTGTACAGGCATTTGATTGCCTTTGCTGTTATTGCACCATTCGCACTAGTTCTTGAAAGGAAAATAAGGCCAAAAATGACTCTGGGGATCTTCCTAAGAATAATGGTGCTTGGTTTTCTCGAGCCTGTTTTTGCTCAGAACTTGTACTTTGTGGGAATGACATATACCTCAGCAACATTTGCATCTGCCACTGCTAATATCCTCCCTGCCATTACCTTCATTTTTGCACTTATTTTCAGGTTAGAAAAAGTCAATTTCAAGAAGCTGCCTAGTGTAGCAAAGGTGATCGGGACTGCAATCACAGTCACAGGAGCGATGGTGATGACATGGTACAAAGGTCCCATCATTGAATTTATAAGTGGACAAGGACAAAGCCACCACACCAGCACCACATCCGGCGAACAACATTGGGTCACCGGCACTATGATGCTCCTAGCCCGTTGTTGCGGTTGGTCCGGCTTCTTCATTGTACAATCTTTCACACTAAAGCTATACCCAGCCGAGCTCTCTCTCACTGCTTTGATATGTTTAATGGGTGCATTGGAAGGGGCCATAGCCACGTTCGTAGTCGAACACAGCATGAGTGTTTGGGTTATAGGCTGGGACTACAGGCTTCTTGCTGCTGCTTACTCTGGGATAGTTTGCTCTGGAATTGCATATTATGTGCAAGGTATTGTGATGAAGGAACAAGGTCCAGTTTTCGTGACAGCTTTCAGCCCACTGGCCATGATCATCACCGCCGCTCTAGCCGCCATCATTTTAGCTGAGCAAGTCCGCCTCGGAAGTATACTTGGAACTATTCTCATAGTCATCGGCCTCTATGCTGTTGTTTGGGGTAAAAGCAAAGATCCTACAACCTCTGCCTTATTGATCAAAGACGAGAAAGCTGTTGCTCATGAATTGCCAATCACGGACAGTAAAAGGTCATCGATCACTGTGGATAACAACACTAAGGATAGTACTACTGGAACTGCTGGGGACTTCAAGTTTCCAATCAAAGCACAAGAATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0032973 GO:0034220 GO:0042592 GO:0043090 GO:0044464 GO:0046942 GO:0046943 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055081 GO:0055085 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071944 GO:0080144 GO:0098656 GO:0140115 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

389

Amino Acids

42.29

Weight (kDa)

9.43

Isoelectric Point (pI)

27.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	22 - 159	1.1e-12	EamA-like transporter family
EamA	PF00892	191 - 328	3.4e-09	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003116)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21890 AT1G21890 AT1G44800 AT4G08300
fragaria_vesca	FvH4_2g26691 FvH4_2g26691 FvH4_2g26691 FvH4_2g26700
malus_domestica	MD00G1157200.v1.1 MD15G1351700.v1.1 MD15G1351800.v1.1
prunus_persica	Prupe.1G500400_v2.0.a1 Prupe.1G500400_v2.0.a1 Prupe.1G500500_v2.0.a1
pyrus_communis	pycom08g14190 pycom15g31510
rosa_chinensis	RchiOBHm_Chr6g0295541 RchiOBHm_Chr6g0295571
rosa_laevigata	RLG00000011789 RLG00000011792
rosa_multiflora	Rmu_sc0005000.1_g000009 Rmu_sc0005000.1_g000012
rosa_roxburghii	Rroxscaffold_7G00172360 Rroxscaffold_7G00172380
rosa_rugosa	Rorug06G0251800 Rorug06G0251900 Rorug06G0252000 Rorug06G0252100
rosa_samantha	Rh6AG365000 Rh6AG365200 Rh6BG373400 Rh6CG379600 Rh6CG379800 Rh6DG366100 Rh6DG366400
rosa_wichuraiana	Rw6G031880 Rw6G031890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	509
AccBSI	CCGCTC	1 cut(s)	896
AccI	GTMKAC	1 cut(s)	940
AciI	CCGC	5 cut(s)	600, 891, 894, 903, 928
AclWI	GGATC	2 cut(s)	248, 995
AcoI	YGGCCR	1 cut(s)	876
AcsI	RAATTY	1 cut(s)	503
AfaI	GTAC	5 cut(s)	158, 299, 485, 624, 1114
AgsI	TTSAA	5 cut(s)	31, 209, 412, 503, 1138
AhlI	ACTAGT	1 cut(s)	199
AluBI	AGCT	6 cut(s)	418, 643, 658, 863, 917, 1040
AluI	AGCT	6 cut(s)	418, 643, 658, 863, 917, 1040
Alw21I	GWGCWC	1 cut(s)	660
AlwI	GGATC	2 cut(s)	248, 995
Ama87I	CYCGRG	1 cut(s)	272
AoxI	GGCC	4 cut(s)	221, 702, 876, 967
ApeKI	GCWGC	3 cut(s)	418, 773, 776
ApoI	RAATTY	1 cut(s)	503
AspS9I	GGNCC	4 cut(s)	491, 606, 702, 845
AsuHPI	GGTGA	4 cut(s)	448, 484, 559, 880
AvaI	CYCGRG	1 cut(s)	272
AvaII	GGWCC	3 cut(s)	491, 606, 845
BaeI	ACNNNNGTAYC	2 cut(s)	475, 508
BalI	TGGCCA	1 cut(s)	878
BanII	GRGCYC	1 cut(s)	660
Bbv12I	GWGCWC	1 cut(s)	660
BbvCI	CCTCAGC	1 cut(s)	322
BbvI	GCAGC	3 cut(s)	405, 760, 763
BccI	CCATC	3 cut(s)	463, 503, 914
BclI	TGATCA	2 cut(s)	882, 1023
BcuI	ACTAGT	1 cut(s)	199
BfaI	CTAG	4 cut(s)	200, 423, 587, 899
BfmI	CTRYAG	1 cut(s)	760
BisI	GCNGC	5 cut(s)	419, 774, 777, 894, 903
BlpI	GCTNAGC	1 cut(s)	918
BlsI	GCNGC	5 cut(s)	420, 775, 778, 895, 904
BmcAI	AGTACT	1 cut(s)	1114
Bme18I	GGWCC	3 cut(s)	491, 606, 845
BmeT110I	CYCGRG	1 cut(s)	272
BmgT120I	GGNCC	4 cut(s)	491, 606, 702, 845
BmiI	GGNNCC	2 cut(s)	493, 703
BmsI	GCATC	3 cut(s)	141, 344, 570
Bpu10I	CCTNAGC	1 cut(s)	322
Bpu1102I	GCTNAGC	1 cut(s)	918
Bsa29I	ATCGAT	1 cut(s)	1082
BsaAI	YACGTR	1 cut(s)	61
BsaJI	CCNNGG	1 cut(s)	931
BsaXI	ACNNNNNCTCC	2 cut(s)	456, 486
Bse118I	RCCGGY	1 cut(s)	569
Bse1I	ACTGG	3 cut(s)	848, 879, 1123
BseCI	ATCGAT	1 cut(s)	1082
BseDI	CCNNGG	1 cut(s)	931
BseGI	GGATG	1 cut(s)	545
BseMII	CTCAG	4 cut(s)	121, 302, 336, 909
BseNI	ACTGG	3 cut(s)	848, 879, 1123
BseXI	GCAGC	3 cut(s)	405, 760, 763
BseYI	CCCAGC	3 cut(s)	649, 753, 1127
BshFI	GGCC	4 cut(s)	223, 704, 878, 969
BshVI	ATCGAT	1 cut(s)	1082
BsiHKAI	GWGCWC	1 cut(s)	660
BsiHKCI	CYCGRG	1 cut(s)	272
BsiSI	CCGG	3 cut(s)	549, 570, 609
BslFI	GGGAC	4 cut(s)	457, 477, 770, 1145
BsmFI	GGGAC	4 cut(s)	457, 477, 770, 1145
BsnI	GGCC	4 cut(s)	223, 704, 878, 969
BsoBI	CYCGRG	1 cut(s)	272
Bsp1286I	GDGCHC	1 cut(s)	660
Bsp1407I	TGTACA	2 cut(s)	156, 622
Bsp143I	GATC	6 cut(s)	240, 438, 882, 1000, 1023, 1083
Bsp1720I	GCTNAGC	1 cut(s)	918
BspACI	CCGC	5 cut(s)	600, 891, 894, 903, 928
BspANI	GGCC	4 cut(s)	223, 704, 878, 969
BspCNI	CTCAG	4 cut(s)	120, 301, 335, 910
BspDI	ATCGAT	1 cut(s)	1082
BspHI	TCATGA	2 cut(s)	1048, 1163
BspLI	GGNNCC	2 cut(s)	493, 703
BspPI	GGATC	2 cut(s)	248, 995
BsrBI	CCGCTC	1 cut(s)	896
BsrFI	RCCGGY	1 cut(s)	569
BsrGI	TGTACA	2 cut(s)	156, 622
BsrI	ACTGG	3 cut(s)	848, 879, 1123
BssAI	RCCGGY	1 cut(s)	569
BssECI	CCNNGG	1 cut(s)	931
BssMI	GATC	6 cut(s)	240, 438, 882, 1000, 1023, 1083
BssNAI	GTATAC	1 cut(s)	941
Bst1107I	GTATAC	1 cut(s)	941
Bst4CI	ACNGT	3 cut(s)	457, 1070, 1090
BstAPI	GCANNNNNTGC	1 cut(s)	332
BstAUI	TGTACA	2 cut(s)	156, 622
BstBAI	YACGTR	1 cut(s)	61
BstDEI	CTNAG	6 cut(s)	107, 248, 288, 322, 918, 1104
BstEII	GGTNACC	1 cut(s)	565
BstF5I	GGATG	1 cut(s)	545
BstKTI	GATC	6 cut(s)	243, 441, 885, 1003, 1026, 1086
BstMBI	GATC	6 cut(s)	240, 438, 882, 1000, 1023, 1083
BstMWI	GCNNNNNNNGC	5 cut(s)	185, 194, 332, 899, 902
BstPI	GGTNACC	1 cut(s)	565
BstSFI	CTRYAG	1 cut(s)	760
BstV1I	GCAGC	3 cut(s)	405, 760, 763
BstX2I	RGATCY	2 cut(s)	240, 1000
BstYI	RGATCY	2 cut(s)	240, 1000
BstZ17I	GTATAC	1 cut(s)	941
Bsu15I	ATCGAT	1 cut(s)	1082
BsuRI	GGCC	4 cut(s)	223, 704, 878, 969
BsuTUI	ATCGAT	1 cut(s)	1082
BtgZI	GCGATG	1 cut(s)	482
BtsCI	GGATG	1 cut(s)	545
BtsI	GCAGTG	2 cut(s)	342, 666
BtsIMutI	CAGTG	4 cut(s)	342, 666, 872, 1086
CciI	TCATGA	2 cut(s)	1048, 1163
Cfr10I	RCCGGY	1 cut(s)	569
Cfr13I	GGNCC	4 cut(s)	491, 606, 702, 845
ClaI	ATCGAT	1 cut(s)	1082
Csp6I	GTAC	5 cut(s)	157, 298, 484, 623, 1113
CviAII	CATG	6 cut(s)	112, 480, 733, 880, 1049, 1164
CviQI	GTAC	5 cut(s)	157, 298, 484, 623, 1113
DdeI	CTNAG	6 cut(s)	107, 248, 288, 322, 918, 1104
DpnI	GATC	6 cut(s)	242, 440, 884, 1002, 1025, 1085
DpnII	GATC	6 cut(s)	240, 438, 882, 1000, 1023, 1083
EaeI	YGGCCR	1 cut(s)	876
EciI	GGCGGA	1 cut(s)	917
Ecl136II	GAGCTC	1 cut(s)	658
Eco24I	GRGCYC	1 cut(s)	660
Eco47I	GGWCC	3 cut(s)	491, 606, 845
Eco53kI	GAGCTC	1 cut(s)	658
Eco88I	CYCGRG	1 cut(s)	272
Eco91I	GGTNACC	1 cut(s)	565
EcoICRI	GAGCTC	1 cut(s)	658
EcoO109I	RGGNCCY	1 cut(s)	491
EcoO65I	GGTNACC	1 cut(s)	565
EcoT38I	GRGCYC	1 cut(s)	660
FaeI	CATG	6 cut(s)	115, 483, 736, 883, 1052, 1167
FaqI	GGGAC	4 cut(s)	457, 477, 770, 1145
FatI	CATG	6 cut(s)	111, 479, 732, 879, 1048, 1163
FbaI	TGATCA	2 cut(s)	882, 1023
FblI	GTMKAC	1 cut(s)	940
Fnu4HI	GCNGC	5 cut(s)	419, 774, 777, 894, 903
FokI	GGATG	1 cut(s)	532
FriOI	GRGCYC	1 cut(s)	660
Fsp4HI	GCNGC	5 cut(s)	419, 774, 777, 894, 903
FspBI	CTAG	4 cut(s)	200, 423, 587, 899
GluI	GCNGC	5 cut(s)	419, 774, 777, 894, 903
GsaI	CCCAGC	3 cut(s)	653, 757, 1131
HaeIII	GGCC	4 cut(s)	223, 704, 878, 969
HapII	CCGG	3 cut(s)	549, 570, 609
Hin1II	CATG	6 cut(s)	115, 483, 736, 883, 1052, 1167
HinfI	GANTC	3 cut(s)	31, 232, 1160
HpaII	CCGG	3 cut(s)	549, 570, 609
HphI	GGTGA	4 cut(s)	448, 484, 559, 880
Hpy166II	GTNNAC	3 cut(s)	157, 515, 941
Hpy188I	TCNGA	2 cut(s)	291, 935
Hpy188III	TCNNGA	8 cut(s)	206, 272, 412, 442, 801, 856, 1049, 1164
Hpy8I	GTNNAC	3 cut(s)	157, 515, 941
HpyAV	CCTTC	3 cut(s)	379, 692, 829
HpyCH4III	ACNGT	3 cut(s)	457, 1070, 1090
HpyCH4IV	ACGT	2 cut(s)	60, 713
HpyF10VI	GCNNNNNNNGC	5 cut(s)	185, 194, 332, 899, 902
HpyF3I	CTNAG	6 cut(s)	107, 248, 288, 322, 918, 1104
HpySE526I	ACGT	2 cut(s)	60, 713
Hsp92II	CATG	6 cut(s)	115, 483, 736, 883, 1052, 1167
Ksp22I	TGATCA	2 cut(s)	882, 1023
Kzo9I	GATC	6 cut(s)	240, 438, 882, 1000, 1023, 1083
LmnI	GCTCC	2 cut(s)	464, 588
Lsp1109I	GCAGC	3 cut(s)	405, 760, 763
LweI	GCATC	3 cut(s)	141, 344, 570
MaeI	CTAG	4 cut(s)	200, 423, 587, 899
MaeII	ACGT	2 cut(s)	60, 713
MaeIII	GTNAC	3 cut(s)	457, 565, 856
MalI	GATC	6 cut(s)	242, 440, 884, 1002, 1025, 1085
MbiI	CCGCTC	1 cut(s)	896
MboI	GATC	6 cut(s)	240, 438, 882, 1000, 1023, 1083
MboII	GAAGA	3 cut(s)	32, 235, 607
MflI	RGATCY	2 cut(s)	240, 1000
MhlI	GDGCHC	1 cut(s)	660
MlsI	TGGCCA	1 cut(s)	878
MluCI	AATT	6 cut(s)	26, 80, 406, 503, 804, 1052
MluNI	TGGCCA	1 cut(s)	878
MlyI	GAGTC	1 cut(s)	226
MnlI	CCTC	7 cut(s)	14, 117, 331, 365, 941, 980, 1021
Mox20I	TGGCCA	1 cut(s)	878
MscI	TGGCCA	1 cut(s)	878
MseI	TTAA	1 cut(s)	683
MslI	CAYNNNNRTG	1 cut(s)	578
Msp20I	TGGCCA	1 cut(s)	878
MspI	CCGG	3 cut(s)	549, 570, 609
MwoI	GCNNNNNNNGC	5 cut(s)	185, 194, 332, 899, 902
NdeII	GATC	6 cut(s)	240, 438, 882, 1000, 1023, 1083
NlaIII	CATG	6 cut(s)	115, 483, 736, 883, 1052, 1167
NlaIV	GGNNCC	2 cut(s)	493, 703
NmeAIII	GCCGAG	1 cut(s)	679
NmuCI	GTSAC	3 cut(s)	457, 565, 856
PaeR7I	CTCGAG	1 cut(s)	272
PagI	TCATGA	2 cut(s)	1048, 1163
PfeI	GAWTC	2 cut(s)	31, 1160
PkrI	GCNGC	5 cut(s)	420, 775, 778, 895, 904
PleI	GAGTC	1 cut(s)	226
PpsI	GAGTC	1 cut(s)	226
Ppu21I	YACGTR	1 cut(s)	61
PpuMI	RGGWCCY	1 cut(s)	491
PsiI	TTATAA	1 cut(s)	509
Psp124BI	GAGCTC	1 cut(s)	660
Psp5II	RGGWCCY	1 cut(s)	491
PspEI	GGTNACC	1 cut(s)	565
PspFI	CCCAGC	3 cut(s)	649, 753, 1127
PspN4I	GGNNCC	2 cut(s)	493, 703
PspPI	GGNCC	4 cut(s)	491, 606, 702, 845
PspPPI	RGGWCCY	1 cut(s)	491
PsuI	RGATCY	2 cut(s)	240, 1000
RsaI	GTAC	5 cut(s)	158, 299, 485, 624, 1114
RsaNI	GTAC	5 cut(s)	157, 298, 484, 623, 1113
RseI	CAYNNNNRTG	1 cut(s)	578
SacI	GAGCTC	1 cut(s)	660
SaqAI	TTAA	1 cut(s)	683
SatI	GCNGC	5 cut(s)	419, 774, 777, 894, 903
Sau3AI	GATC	6 cut(s)	240, 438, 882, 1000, 1023, 1083
Sau96I	GGNCC	4 cut(s)	491, 606, 702, 845
ScaI	AGTACT	1 cut(s)	1114
SchI	GAGTC	1 cut(s)	226
SduI	GDGCHC	1 cut(s)	660
SfaNI	GCATC	3 cut(s)	141, 344, 570
SfcI	CTRYAG	1 cut(s)	760
Sfr274I	CTCGAG	1 cut(s)	272
SinI	GGWCC	3 cut(s)	491, 606, 845
SlaI	CTCGAG	1 cut(s)	272
SmiMI	CAYNNNNRTG	1 cut(s)	578
SmlI	CTYRAG	1 cut(s)	272
SmoI	CTYRAG	1 cut(s)	272
SpeI	ACTAGT	1 cut(s)	199
Sse9I	AATT	6 cut(s)	26, 80, 406, 503, 804, 1052
SsiI	CCGC	5 cut(s)	600, 891, 894, 903, 928
SspMI	CTAG	4 cut(s)	200, 423, 587, 899
SstI	GAGCTC	1 cut(s)	660
TaaI	ACNGT	3 cut(s)	457, 1070, 1090
TaiI	ACGT	2 cut(s)	63, 716
TaqI	TCGA	3 cut(s)	273, 723, 1082
TasI	AATT	6 cut(s)	26, 80, 406, 503, 804, 1052
TatI	WGTACW	4 cut(s)	156, 297, 622, 1112
TauI	GCSGC	2 cut(s)	896, 905
TfiI	GAWTC	2 cut(s)	31, 1160
Tru1I	TTAA	1 cut(s)	683
Tru9I	TTAA	1 cut(s)	683
TscAI	CASTG	4 cut(s)	349, 673, 879, 1093
TseFI	GTSAC	3 cut(s)	457, 565, 856
TseI	GCWGC	3 cut(s)	418, 773, 776
Tsp45I	GTSAC	3 cut(s)	457, 565, 856
TspDTI	ATGAA	4 cut(s)	361, 607, 848, 1065
TspGWI	ACGGA	1 cut(s)	1079
TspRI	CASTG	4 cut(s)	349, 673, 879, 1093
VpaK11BI	GGWCC	3 cut(s)	491, 606, 845
XapI	RAATTY	1 cut(s)	503
XhoI	CTCGAG	1 cut(s)	272
XmiI	GTMKAC	1 cut(s)	940
XspI	CTAG	4 cut(s)	200, 423, 587, 899
ZrmI	AGTACT	1 cut(s)	1114

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.