Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003366

Physical Location & Seq

Genomic Coordinates Forward (+)

38161476 .. 38163482

Gene Structure 2007 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1166500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2007 bp

ATGACGAAACGACCCTTCTCCTCCCTTACCACCGCCACCTTCATCATCATCACCGCCACCATCCTCTTCGCCGTTATACCACCACAAGCTCATGCCTTCGGTTCCGGCTCCACCCTTGCCGTTATCTCCGCCTCCGGCACCGTCTGCGGTGTCGTGTCAGACCAACCGCGGCAGCGCATCGTCTGCTACCGCCGCGGACAAACCGTCGCAGTCGAGCCCAACATCTCCTTCTCCGCCATCTCCGGCGGACGGACCTCTCTATGCGGCCTCCGCTCTGGCGGATACAGCATCCTCTGCTGGGACGACATCGCCTCCCAAAACTCCACCTTCCCATCCAAACGTCTTTACTTCAACAAAACCGTTACGTTAGAAAATCTCTCAGTCGGCGACGACCATATCTGCGCCAGAACCGTCGTCACAGGCACCATAAAATGCTGGCGGGTCGACGACGTGTCCGAATTACCATCTGGGTCGGACCAATTCTCCTCGATTTCATCGGGGTCGGGTTTCTCTTGCGGAATTTCCAAAGCCACCCTCCGGGTTCGATGCTGGGGCAGAGCAACTTCAATGGCGGAAGAAATACAAACCGGGTTCGGAAACATGACAATGGCGACCATCACAGCCGGCGGCTCGCACGTGTGCGGGATCAACACCACAGGACTTATAGTCTGCCGGGGAAGCAATGGCGCCGGCCAATGGAACGTCCCTCCTGGCTCGCCTTTCGAGTTCTCTGCTCTGTCCCTCGGAGTAAACCACAGCTGCGCAATCAGAACTTCAAATAAAACGGTCATTTGTTGGGGAGGAGGAGGTCAATTTGCATTTAACCAAACAAACGGGGTGTCTTTTGAAGTAATTGCTTCTGGGTCGGATTTCGTTTGTGGATTAACCACCAGGAATTTTTCGATTGTTTGTTGGGGTACGGGCTGGCCCAATCTCTCCGGTTTGAAATCAGGGGAGGAGCTCGAATTGCCCGAAATTCTTCCCGGTTCTTGTGTCAATTCCTCCTGCGGTGAATGTGGAATTTACCCTCAGTCCCAAAGGCTCTGTTTTGGTGCAGACAACATTTGCAAGCCCTGTTTGCCTCCAATTTCATTGCCACCACCAGCATTTCCACCGCCGCCGCCGTCGCCGCAGCCGCCACCGCCCCTGCCGTCAAGGCTGCCGCCCGCGGCATTGACGACGGGTTTATTGATGTTTGCCATTGTCGGTTCTGTCGGAGCTTTTGCAGGTATTTGTACTGTTATTTATTGTTTGTGGACCGGCGGTTGTTTTGGGGACAAAAAGATTCACAATTCAGTTCAGCCGACAATTACTAGAGATGCTAGCTCAAACAATGGCAATGGGATAACGTCAAACAATAGTCCCCCGTCGAGATCCTCCACCATCCGCCGCCAGGGCTCGAGGATCATGAGGCGGCAGAGGAGCGGAACATCCTCGAAGCATGCAGACAGGGCGGAGGAGTTTTCCCTGTCCGAGCTCGCGGCCGCCACCAATGACTTCTCAATGGAGAACAAGATTGGCGCCGGAAGCTTCGGAGTTGTATATAGAGGCAAACTGCCTGACGGCCGGGAAGTTGCGATCAAGAGAGGCGAAACCGCCCCGAAGATGAAGAAGTATCAGGAGAAGGAGAGCGCATTCGATTCGGAACTTGCGTTCTTGTCGAGAGTTCATCACAAACATCTCGTGAGGCTTGTCGGATATTGCCAAGAGAGGGACGAGAGGCTTTTGGTGTACGAGCACGTGAAAAACGGTGCGCTTTATGATCACTTGCATGACAAGAACAATGTGGAGAGAGAAGCAGTAGTTTTGTTAAATTCGTGGAAAATGAGGATCAAGGTTGCATTGGATGCCTCCAGAGGCATTGAGTATCTGCACAATTACGCCGTGCCGCCTATCATTCATCGAGATATAAAGTCATCGAACATCTTGCTCGACGCGAATTGGACCGCGAGGGTGTCTGATTTTGGGTTGTCGTTGATGGGGCCGGAGTCAGAGGTGGATTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr RCC1_2

Protein Analysis

669

Amino Acids

71.73

Weight (kDa)

8.66

Isoelectric Point (pI)

46.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	501 - 658	1.4e-28	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	501 - 658	2.7e-28	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55950
fragaria_vesca	FvH4_3g38710 FvH4_7g24490
malus_domestica	MD00G1166500.v1.1 MD01G1153100.v1.1 MD03G1068800.v1.1 MD07G1222100.v1.1 MD11G1073300.v1.1
prunus_persica	Prupe.2G250300_v2.0.a1 Prupe.6G056600_v2.0.a1
pyrus_communis	pycom01g17070 pycom03g05510
rosa_chinensis	RchiOBHm_Chr1g0370251 RchiOBHm_Chr5g0065501 RchiOBHm_Chr5g0069861
rosa_laevigata	RLG00000027065 RLG00000036103
rosa_multiflora	Rmu_co8321277.1_g000001 Rmu_sc0000738.1_g000002 Rmu_sc0026893.1_g000001 Rmu_sc0037931.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011190 Rroxscaffold_4G00286710 Rroxscaffold_4G00287300
rosa_rugosa	Rorug01G0355200 Rorug05G0400300
rosa_samantha	Rh1AG363800 Rh1BG326900 Rh1CG340200 Rh1DG357300 Rh5AG457400 Rh5BG476200 Rh5CG500000 Rh5DG488000
rosa_wichuraiana	Rw1G032010 Rw5G042700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	763
Acc36I	ACCTGC	1 cut(s)	1217
AccB1I	GGYRCC	4 cut(s)	137, 422, 686, 1520
AccBSI	CCGCTC	2 cut(s)	273, 1425
AccI	GTMKAC	1 cut(s)	444
AccII	CGCG	6 cut(s)	169, 195, 1169, 1481, 1937, 1949
AclWI	GGATC	4 cut(s)	653, 1368, 1412, 1838
AcoI	YGGCCR	3 cut(s)	691, 1482, 1564
AcsI	RAATTY	5 cut(s)	519, 895, 975, 1020, 1813
AcvI	CACGTG	2 cut(s)	637, 1741
AcyI	GRCGYC	2 cut(s)	687, 1521
AfaI	GTAC	3 cut(s)	919, 1237, 1733
AfiI	CCNNNNNNNGG	6 cut(s)	249, 298, 537, 696, 1393, 1711
AflIII	ACRYGT	2 cut(s)	450, 636
AgsI	TTSAA	5 cut(s)	352, 567, 777, 848, 946
AhdI	GACNNNNNGTC	3 cut(s)	203, 451, 665
AjiI	CACGTC	1 cut(s)	451
AjnI	CCWGG	3 cut(s)	709, 890, 1392
AjuI	GAANNNNNNNTTGG	2 cut(s)	212, 244
AloI	GAACNNNNNNTCC	2 cut(s)	1637, 1669
AluBI	AGCT	7 cut(s)	89, 759, 961, 1220, 1326, 1477, 1530
AluI	AGCT	7 cut(s)	89, 759, 961, 1220, 1326, 1477, 1530
Alw21I	GWGCWC	3 cut(s)	963, 1479, 1740
AlwI	GGATC	4 cut(s)	653, 1368, 1412, 1838
Ama87I	CYCGRG	1 cut(s)	1399
AoxI	GGCC	6 cut(s)	265, 691, 926, 1482, 1564, 1982
ApeKI	GCWGC	4 cut(s)	172, 759, 1132, 1159
ApoI	RAATTY	5 cut(s)	519, 895, 975, 1020, 1813
ArsI	GACNNNNNNTTYG	2 cut(s)	824, 856
Asp700I	GAANNNNTTC	1 cut(s)	978
AspLEI	GCGC	7 cut(s)	177, 404, 689, 764, 1523, 1634, 1756
AspS9I	GGNCC	6 cut(s)	252, 475, 927, 1257, 1944, 1982
AsuC2I	CCSGG	5 cut(s)	539, 589, 674, 984, 1568
AsuHPI	GGTGA	2 cut(s)	43, 1022
AsuNHI	GCTAGC	1 cut(s)	1322
AvaI	CYCGRG	1 cut(s)	1399
AvaII	GGWCC	4 cut(s)	252, 475, 1257, 1944
BanI	GGYRCC	4 cut(s)	137, 422, 686, 1520
BanII	GRGCYC	4 cut(s)	219, 963, 1400, 1479
BauI	CACGAG	1 cut(s)	1682
BbrPI	CACGTG	2 cut(s)	637, 1741
Bbv12I	GWGCWC	3 cut(s)	963, 1479, 1740
BbvI	GCAGC	4 cut(s)	184, 746, 1144, 1146
BccI	CCATC	7 cut(s)	68, 245, 340, 472, 623, 1391, 1972
BceAI	ACGGC	6 cut(s)	56, 104, 1108, 1135, 1579, 1868
BcgI	CGANNNNNNTGC	6 cut(s)	713, 747, 1623, 1657, 1909, 1943
BciT130I	CCWGG	3 cut(s)	711, 892, 1394
BciVI	GTATCC	1 cut(s)	275
BclI	TGATCA	1 cut(s)	1762
BcnI	CCSGG	5 cut(s)	539, 589, 674, 984, 1568
BfaI	CTAG	2 cut(s)	1314, 1323
BfoI	RGCGCY	2 cut(s)	690, 1524
BfuAI	ACCTGC	1 cut(s)	1217
BfuI	GTATCC	1 cut(s)	275
BglI	GCCNNNNNGGC	2 cut(s)	1156, 1395
Bme1390I	CCNGG	8 cut(s)	539, 589, 674, 711, 892, 984, 1394, 1568
Bme18I	GGWCC	4 cut(s)	252, 475, 1257, 1944
BmeRI	GACNNNNNGTC	3 cut(s)	203, 451, 665
BmeT110I	CYCGRG	1 cut(s)	1399
BmgBI	CACGTC	1 cut(s)	451
BmgT120I	GGNCC	6 cut(s)	252, 475, 927, 1257, 1944, 1982
BmiI	GGNNCC	7 cut(s)	103, 109, 139, 424, 688, 1522, 1983
BmrFI	CCNGG	8 cut(s)	539, 589, 674, 711, 892, 984, 1394, 1568
BmsI	GCATC	5 cut(s)	186, 297, 536, 1309, 1837
BmtI	GCTAGC	1 cut(s)	1326
BpmI	CTGGAG	1 cut(s)	1837
BpuMI	CCSGG	5 cut(s)	539, 589, 674, 984, 1568
BsaAI	YACGTR	2 cut(s)	637, 1741
BsaHI	GRCGYC	2 cut(s)	687, 1521
BsaJI	CCNNGG	6 cut(s)	167, 193, 673, 742, 1167, 1393
BsaWI	WCCGGW	1 cut(s)	938
BsaXI	ACNNNNNCTCC	4 cut(s)	296, 326, 467, 497
Bsc4I	CCNNNNNNNGG	6 cut(s)	249, 298, 537, 696, 1393, 1711
Bse118I	RCCGGY	3 cut(s)	623, 689, 1259
Bse3DI	GCAATG	3 cut(s)	688, 1091, 1345
BseBI	CCWGG	3 cut(s)	711, 892, 1394
BseDI	CCNNGG	6 cut(s)	167, 193, 673, 742, 1167, 1393
BseGI	GGATG	6 cut(s)	60, 288, 332, 1383, 1430, 1852
BseLI	CCNNNNNNNGG	6 cut(s)	249, 298, 537, 696, 1393, 1711
BseMI	GCAATG	3 cut(s)	688, 1091, 1345
BseMII	CTCAG	2 cut(s)	393, 1043
BseRI	GAGGAG	7 cut(s)	10, 475, 816, 819, 971, 1435, 1472
BseX3I	CGGCCG	2 cut(s)	1482, 1564
BseXI	GCAGC	4 cut(s)	184, 746, 1144, 1146
BseYI	CCCAGC	2 cut(s)	297, 549
BsgI	GTGCAG	2 cut(s)	1074, 1856
Bsh1236I	CGCG	6 cut(s)	169, 195, 1169, 1481, 1937, 1949
Bsh1285I	CGRYCG	2 cut(s)	1485, 1567
BshFI	GGCC	6 cut(s)	267, 693, 928, 1484, 1566, 1984
BshNI	GGYRCC	4 cut(s)	137, 422, 686, 1520
BsiEI	CGRYCG	2 cut(s)	1485, 1567
BsiHKAI	GWGCWC	3 cut(s)	963, 1479, 1740
BsiHKCI	CYCGRG	1 cut(s)	1399
BslFI	GGGAC	7 cut(s)	314, 689, 724, 1018, 1289, 1347, 1727
BslI	CCNNNNNNNGG	6 cut(s)	249, 298, 537, 696, 1393, 1711
BsmFI	GGGAC	7 cut(s)	314, 689, 724, 1018, 1289, 1347, 1727
BsmI	GAATGC	1 cut(s)	1634
BsnI	GGCC	6 cut(s)	267, 693, 928, 1484, 1566, 1984
BsoBI	CYCGRG	1 cut(s)	1399
Bsp1286I	GDGCHC	5 cut(s)	219, 963, 1400, 1479, 1740
Bsp143I	GATC	6 cut(s)	645, 1373, 1404, 1578, 1762, 1830
BspANI	GGCC	6 cut(s)	267, 693, 928, 1484, 1566, 1984
BspCNI	CTCAG	2 cut(s)	392, 1042
BspFNI	CGCG	6 cut(s)	169, 195, 1169, 1481, 1937, 1949
BspHI	TCATGA	1 cut(s)	1407
BspLI	GGNNCC	7 cut(s)	103, 109, 139, 424, 688, 1522, 1983
BspMI	ACCTGC	1 cut(s)	1217
BspOI	GCTAGC	1 cut(s)	1326
BspPI	GGATC	4 cut(s)	653, 1368, 1412, 1838
BspT107I	GGYRCC	4 cut(s)	137, 422, 686, 1520
BsrBI	CCGCTC	2 cut(s)	273, 1425
BsrDI	GCAATG	3 cut(s)	688, 1091, 1345
BsrFI	RCCGGY	3 cut(s)	623, 689, 1259
BssAI	RCCGGY	3 cut(s)	623, 689, 1259
BssECI	CCNNGG	6 cut(s)	167, 193, 673, 742, 1167, 1393
BssMI	GATC	6 cut(s)	645, 1373, 1404, 1578, 1762, 1830
BssNI	GRCGYC	2 cut(s)	687, 1521
BssSI	CACGAG	1 cut(s)	1682
Bst2BI	CACGAG	1 cut(s)	1682
Bst2UI	CCWGG	3 cut(s)	711, 892, 1394
Bst4CI	ACNGT	7 cut(s)	142, 205, 361, 412, 787, 1240, 1751
Bst6I	CTCTTC	1 cut(s)	71
BstACI	GRCGYC	2 cut(s)	687, 1521
BstAPI	GCANNNNNTGC	4 cut(s)	144, 183, 294, 1847
BstBAI	YACGTR	2 cut(s)	637, 1741
BstDEI	CTNAG	2 cut(s)	379, 1029
BstDSI	CCRYGG	3 cut(s)	167, 193, 1167
BstF5I	GGATG	6 cut(s)	60, 288, 332, 1383, 1430, 1852
BstFNI	CGCG	6 cut(s)	169, 195, 1169, 1481, 1937, 1949
BstH2I	RGCGCY	2 cut(s)	690, 1524
BstHHI	GCGC	7 cut(s)	177, 404, 689, 764, 1523, 1634, 1756
BstKTI	GATC	6 cut(s)	648, 1376, 1407, 1581, 1765, 1833
BstMBI	GATC	6 cut(s)	645, 1373, 1404, 1578, 1762, 1830
BstMCI	CGRYCG	2 cut(s)	1485, 1567
BstNI	CCWGG	3 cut(s)	711, 892, 1394
BstNSI	RCATGY	1 cut(s)	1445
BstSCI	CCNGG	8 cut(s)	537, 587, 672, 709, 890, 982, 1392, 1566
BstUI	CGCG	6 cut(s)	169, 195, 1169, 1481, 1937, 1949
BstV1I	GCAGC	4 cut(s)	184, 746, 1144, 1146
BstX2I	RGATCY	1 cut(s)	1373
BstYI	RGATCY	1 cut(s)	1373
BstZI	CGGCCG	2 cut(s)	1482, 1564
BsuI	GTATCC	1 cut(s)	275
BsuRI	GGCC	6 cut(s)	267, 693, 928, 1484, 1566, 1984
BtgI	CCRYGG	3 cut(s)	167, 193, 1167
BtgZI	GCGATG	1 cut(s)	292
BtrI	CACGTC	1 cut(s)	451
BtsCI	GGATG	6 cut(s)	60, 288, 332, 1383, 1430, 1852
BveI	ACCTGC	1 cut(s)	1217
CciI	TCATGA	1 cut(s)	1407
CciNI	GCGGCCGC	1 cut(s)	1482
CfoI	GCGC	7 cut(s)	177, 404, 689, 764, 1523, 1634, 1756
Cfr10I	RCCGGY	3 cut(s)	623, 689, 1259
Cfr13I	GGNCC	6 cut(s)	252, 475, 927, 1257, 1944, 1982
Cfr42I	CCGCGG	3 cut(s)	170, 196, 1170
CseI	GACGC	1 cut(s)	1943
Csp6I	GTAC	3 cut(s)	918, 1236, 1732
CviAII	CATG	5 cut(s)	92, 601, 1408, 1442, 1772
CviQI	GTAC	3 cut(s)	918, 1236, 1732
DdeI	CTNAG	2 cut(s)	379, 1029
DinI	GGCGCC	2 cut(s)	688, 1522
DpnI	GATC	6 cut(s)	647, 1375, 1406, 1580, 1764, 1832
DpnII	GATC	6 cut(s)	645, 1373, 1404, 1578, 1762, 1830
DriI	GACNNNNNGTC	3 cut(s)	203, 451, 665
EaeI	YGGCCR	3 cut(s)	691, 1482, 1564
EagI	CGGCCG	2 cut(s)	1482, 1564
Eam1104I	CTCTTC	1 cut(s)	71
Eam1105I	GACNNNNNGTC	3 cut(s)	203, 451, 665
EarI	CTCTTC	1 cut(s)	71
EciI	GGCGGA	7 cut(s)	118, 223, 261, 294, 587, 1376, 1469
Ecl136II	GAGCTC	2 cut(s)	961, 1477
EclXI	CGGCCG	2 cut(s)	1482, 1564
Eco24I	GRGCYC	4 cut(s)	219, 963, 1400, 1479
Eco47I	GGWCC	4 cut(s)	252, 475, 1257, 1944
Eco52I	CGGCCG	2 cut(s)	1482, 1564
Eco53kI	GAGCTC	2 cut(s)	961, 1477
Eco72I	CACGTG	2 cut(s)	637, 1741
Eco88I	CYCGRG	1 cut(s)	1399
EcoICRI	GAGCTC	2 cut(s)	961, 1477
EcoRII	CCWGG	3 cut(s)	709, 890, 1392
EcoT38I	GRGCYC	4 cut(s)	219, 963, 1400, 1479
EgeI	GGCGCC	2 cut(s)	688, 1522
EheI	GGCGCC	2 cut(s)	688, 1522
FaeI	CATG	5 cut(s)	95, 604, 1411, 1445, 1775
FaqI	GGGAC	7 cut(s)	314, 689, 724, 1018, 1289, 1347, 1727
FatI	CATG	5 cut(s)	91, 600, 1407, 1441, 1771
FauI	CCCGC	3 cut(s)	432, 635, 1174
FbaI	TGATCA	1 cut(s)	1762
FblI	GTMKAC	1 cut(s)	444
FokI	GGATG	6 cut(s)	47, 275, 319, 1370, 1417, 1859
FriOI	GRGCYC	4 cut(s)	219, 963, 1400, 1479
FspBI	CTAG	2 cut(s)	1314, 1323
FspI	TGCGCA	1 cut(s)	763
GlaI	GCGC	7 cut(s)	176, 403, 688, 763, 1522, 1633, 1755
GsaI	CCCAGC	2 cut(s)	301, 553
GsuI	CTGGAG	1 cut(s)	1837
HaeII	RGCGCY	2 cut(s)	690, 1524
HaeIII	GGCC	6 cut(s)	267, 693, 928, 1484, 1566, 1984
HgaI	GACGC	1 cut(s)	1943
HhaI	GCGC	7 cut(s)	177, 404, 689, 764, 1523, 1634, 1756
Hin1I	GRCGYC	2 cut(s)	687, 1521
Hin1II	CATG	5 cut(s)	95, 604, 1411, 1445, 1775
Hin6I	GCGC	7 cut(s)	175, 402, 687, 762, 1521, 1632, 1754
HinP1I	GCGC	7 cut(s)	175, 402, 687, 762, 1521, 1632, 1754
HincII	GTYRAC	1 cut(s)	445
HindII	GTYRAC	1 cut(s)	445
HindIII	AAGCTT	1 cut(s)	1528
HinfI	GANTC	3 cut(s)	1285, 1640, 1988
HphI	GGTGA	2 cut(s)	43, 1022
Hpy166II	GTNNAC	4 cut(s)	445, 751, 1257, 1732
Hpy188III	TCNNGA	8 cut(s)	1371, 1408, 1582, 1619, 1662, 1684, 1854, 1904
Hpy8I	GTNNAC	4 cut(s)	445, 751, 1257, 1732
Hpy99I	CGWCG	9 cut(s)	209, 392, 416, 449, 452, 1129, 1183, 1372, 1937
HpyAV	CCTTC	6 cut(s)	25, 49, 106, 238, 337, 1618
HpyCH4III	ACNGT	7 cut(s)	142, 205, 361, 412, 787, 1240, 1751
HpyCH4IV	ACGT	7 cut(s)	340, 365, 450, 636, 702, 1349, 1740
HpyCH4V	TGCA	8 cut(s)	818, 1055, 1068, 1226, 1445, 1771, 1841, 1873
HpyF3I	CTNAG	2 cut(s)	379, 1029
HpySE526I	ACGT	7 cut(s)	340, 365, 450, 636, 702, 1349, 1740
Hsp92I	GRCGYC	2 cut(s)	687, 1521
Hsp92II	CATG	5 cut(s)	95, 604, 1411, 1445, 1775
HspAI	GCGC	7 cut(s)	175, 402, 687, 762, 1521, 1632, 1754
KasI	GGCGCC	2 cut(s)	686, 1520
KroI	GCCGGC	2 cut(s)	623, 689
KroNI	GCCGGC	2 cut(s)	625, 691
Ksp22I	TGATCA	1 cut(s)	1762
KspI	CCGCGG	3 cut(s)	170, 196, 1170
Kzo9I	GATC	6 cut(s)	645, 1373, 1404, 1578, 1762, 1830
LmnI	GCTCC	4 cut(s)	113, 958, 1217, 1422
Lsp1109I	GCAGC	4 cut(s)	184, 746, 1144, 1146
LweI	GCATC	5 cut(s)	186, 297, 536, 1309, 1837
MaeI	CTAG	2 cut(s)	1314, 1323
MaeII	ACGT	7 cut(s)	340, 365, 450, 636, 702, 1349, 1740
MaeIII	GTNAC	2 cut(s)	361, 415
MalI	GATC	6 cut(s)	647, 1375, 1406, 1580, 1764, 1832
MbiI	CCGCTC	2 cut(s)	273, 1425
MboI	GATC	6 cut(s)	645, 1373, 1404, 1578, 1762, 1830
MboII	GAAGA	5 cut(s)	58, 587, 971, 1615, 1621
MflI	RGATCY	1 cut(s)	1373
MhlI	GDGCHC	5 cut(s)	219, 963, 1400, 1479, 1740
Mly113I	GGCGCC	2 cut(s)	687, 1521
MlyI	GAGTC	1 cut(s)	1997
MmeI	TCCRAC	4 cut(s)	453, 846, 1195, 1675
MroNI	GCCGGC	2 cut(s)	623, 689
MroXI	GAANNNNTTC	1 cut(s)	978
MseI	TTAA	3 cut(s)	822, 884, 1811
MslI	CAYNNNNRTG	2 cut(s)	605, 1770
MspA1I	CMGCKG	4 cut(s)	169, 195, 759, 1169
MspR9I	CCNGG	8 cut(s)	539, 589, 674, 711, 892, 984, 1394, 1568
Mva1269I	GAATGC	1 cut(s)	1634
MvaI	CCWGG	3 cut(s)	711, 892, 1394
MvnI	CGCG	6 cut(s)	169, 195, 1169, 1481, 1937, 1949
NaeI	GCCGGC	2 cut(s)	625, 691
NarI	GGCGCC	2 cut(s)	687, 1521
NciI	CCSGG	5 cut(s)	539, 589, 674, 984, 1568
NdeII	GATC	6 cut(s)	645, 1373, 1404, 1578, 1762, 1830
NgoMIV	GCCGGC	2 cut(s)	623, 689
NheI	GCTAGC	1 cut(s)	1322
NlaIII	CATG	5 cut(s)	95, 604, 1411, 1445, 1775
NlaIV	GGNNCC	7 cut(s)	103, 109, 139, 424, 688, 1522, 1983
NmuCI	GTSAC	1 cut(s)	415
NotI	GCGGCCGC	1 cut(s)	1482
NsbI	TGCGCA	1 cut(s)	763
NspI	RCATGY	1 cut(s)	1445
PaeI	GCATGC	1 cut(s)	1445
PaeR7I	CTCGAG	1 cut(s)	1399
PagI	TCATGA	1 cut(s)	1407
PcsI	WCGNNNNNNNCGW	3 cut(s)	453, 969, 1649
PctI	GAATGC	1 cut(s)	1634
PdiI	GCCGGC	2 cut(s)	625, 691
PdmI	GAANNNNTTC	1 cut(s)	978
PfeI	GAWTC	2 cut(s)	1285, 1640
PleI	GAGTC	1 cut(s)	1996
PluTI	GGCGCC	2 cut(s)	690, 1524
PmaCI	CACGTG	2 cut(s)	637, 1741
PmlI	CACGTG	2 cut(s)	637, 1741
PpsI	GAGTC	1 cut(s)	1996
Ppu21I	YACGTR	2 cut(s)	637, 1741
Psp124BI	GAGCTC	2 cut(s)	963, 1479
Psp6I	CCWGG	3 cut(s)	709, 890, 1392
PspCI	CACGTG	2 cut(s)	637, 1741
PspFI	CCCAGC	2 cut(s)	297, 549
PspGI	CCWGG	3 cut(s)	709, 890, 1392
PspN4I	GGNNCC	7 cut(s)	103, 109, 139, 424, 688, 1522, 1983
PspPI	GGNCC	6 cut(s)	252, 475, 927, 1257, 1944, 1982
PspXI	VCTCGAGB	1 cut(s)	1399
PsuI	RGATCY	1 cut(s)	1373
PvuII	CAGCTG	1 cut(s)	759
RsaI	GTAC	3 cut(s)	919, 1237, 1733
RsaNI	GTAC	3 cut(s)	918, 1236, 1732
RseI	CAYNNNNRTG	2 cut(s)	605, 1770
SacI	GAGCTC	2 cut(s)	963, 1479
SacII	CCGCGG	3 cut(s)	170, 196, 1170
SalI	GTCGAC	1 cut(s)	443
SaqAI	TTAA	3 cut(s)	822, 884, 1811
Sau3AI	GATC	6 cut(s)	645, 1373, 1404, 1578, 1762, 1830
Sau96I	GGNCC	6 cut(s)	252, 475, 927, 1257, 1944, 1982
SchI	GAGTC	1 cut(s)	1997
ScrFI	CCNGG	8 cut(s)	539, 589, 674, 711, 892, 984, 1394, 1568
SduI	GDGCHC	5 cut(s)	219, 963, 1400, 1479, 1740
SfaNI	GCATC	5 cut(s)	186, 297, 536, 1309, 1837
SfoI	GGCGCC	2 cut(s)	688, 1522
Sfr274I	CTCGAG	1 cut(s)	1399
Sfr303I	CCGCGG	3 cut(s)	170, 196, 1170
SgrBI	CCGCGG	3 cut(s)	170, 196, 1170
SinI	GGWCC	4 cut(s)	252, 475, 1257, 1944
SlaI	CTCGAG	1 cut(s)	1399
SmiMI	CAYNNNNRTG	2 cut(s)	605, 1770
SmlI	CTYRAG	1 cut(s)	1399
SmoI	CTYRAG	1 cut(s)	1399
SphI	GCATGC	1 cut(s)	1445
SspDI	GGCGCC	2 cut(s)	686, 1520
SspMI	CTAG	2 cut(s)	1314, 1323
SstI	GAGCTC	2 cut(s)	963, 1479
StyD4I	CCNGG	8 cut(s)	537, 587, 672, 709, 890, 982, 1392, 1566
TaaI	ACNGT	7 cut(s)	142, 205, 361, 412, 787, 1240, 1751
TaiI	ACGT	7 cut(s)	343, 368, 453, 639, 705, 1352, 1743
TatI	WGTACW	1 cut(s)	1235
TfiI	GAWTC	2 cut(s)	1285, 1640
Tru1I	TTAA	3 cut(s)	822, 884, 1811
Tru9I	TTAA	3 cut(s)	822, 884, 1811
TseFI	GTSAC	1 cut(s)	415
TseI	GCWGC	4 cut(s)	172, 759, 1132, 1159
Tsp45I	GTSAC	1 cut(s)	415
TspDTI	ATGAA	6 cut(s)	31, 483, 1080, 1622, 1658, 1889
TspGWI	ACGGA	1 cut(s)	265
VpaK11BI	GGWCC	4 cut(s)	252, 475, 1257, 1944
XapI	RAATTY	5 cut(s)	519, 895, 975, 1020, 1813
XceI	RCATGY	1 cut(s)	1445
XhoI	CTCGAG	1 cut(s)	1399
XmiI	GTMKAC	1 cut(s)	444
XmnI	GAANNNNTTC	1 cut(s)	978
XspI	CTAG	2 cut(s)	1314, 1323

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.