Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0003366

Physical Location & Seq

Genomic Coordinates Reverse (-)

58079981 .. 58082871

Gene Structure 2891 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG357300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2376 bp

ATGACGAAACTACCCTTCCCCGTTCTCATTACCATCCCCACCTTCATCTTCACCATTTTGTTCACCCTCCCATTTTCCCATGCACTCGGCTCCGGCGCCACCCTCGCCGTCAGCTACGCCACCGGCGCCGTCTGCGGCTTGGTCTCCGGCCAACCCACGCAGCGCATAGTCTGCTACCGGCGAGGCCAAACCATCTCCGTCCAGCCTAACATCTCCTTCTTGTCAATCTCCGGCGGCCGGACCTCCCTCTGCGGCCTTCGGTCCGGCGGATACGCACTGCTCTGTTACGACAATCTACTCTTCCAAGACTCTGACTTTGTTCCGAAAAGAGTCTACTTTAATGAAAATGTTGAATTGGAAAATATAGCCGTCGGAGAAAACCATATCTGCGCCACCGTGGTCGGTTCCGGCGCCGTGAAATGTTGGCGAGAGGACGACAGAACGTTTCAACTGCCATCTGGGTCGGACCAATTCGGGTCCATCTCATCTGGGTCGGGCTTCTCCTGTGGGATTTCGAAGAACCGCTCAAGGGTCCGGTGCTGGGGGACTAATACTTCCATGGCGGCCAGTATTGAAAATGGGTTTGGAGAAACGGCCATGGCGAGTATTGAAGCAGGTGCTTATCACGTGTGTGGAGTCAATGTTACTGGGTCAATAATATGCAGAGGCAACAACGACTCTGGTCAATTGAATGCACCGCCGGGAAAGTTTTCCGGTCTAGCTCTCGGAGCTGAACACAGCTGTGCGATTAGAGATCCAAACCAGTCTGTGATTTGTTGGGGCGGTGGAGGCCAGTTTGGGTCTAATGTGACTTCAGCGACGTCGTTTGAGGTGATCGTTTCCGGGTCTAATTTCACTTGTGGATTAACCACAAGCAACTTTTCGATTATATGTTGGGGTCCTGGTTGGCCTAGTTCAGAGGTTGAAGGTAAGCTTGAGTTGCCGGAGATTCTTCCTGGTCGTTGTATACAGGCCTCTTGTAGTGAATGTGGTCTTTATCCTCAATCTCAAAAGCTCTGTTATGGCTCTGAGAACATTTGCAAGCCTTGTTTGCCTTCGAATTTGGTTCCTACATTACCACCGTCACCGTCGATTTCACCGCCTTCGGAATCTCCATCCCGGGCTTTGAAAACGGGGTTGTTAGTGTTCTGCATTGTGGGTTCTATAGGAGGGTTTTCGGGGATTTGTAGTGTGATTTATTGTTTGTGGATGGGTGGTTGTTTTGGGCAAAAGAAAGTTCATAATTCAGTTCAGCCTACAATCACCAGAGCTGGCAGCTCCAACCACGGGAACACATCAAATAACAGCCCTCCTTCGAGATCATCCACCATAAGGCGGCAGAGCTCGAGGATCATGAGGCGGCAGAGGAGTGGGCCTTCGTCGAAGCACCCAGATAGGGCTGAGGAGTTCTCACTGGCTGAGCTTGCAGCAGCTACAAATGGTTTTGCATTGGAGAACAAGATTGGTGCAGGGAGCTTTGGGGTGGTGTACAGGGGGAAATTGGATGACGGGCGCGAAGTTGCTATCAAGAGAGGAGAAACCGCAACCAAGGTGAAGAAGTACCAAGAGAAGGATACTGCTTTCGACTCGGAGTTGTCATTCTTGTCGAGGCTTCATCACAAGCATTTGGTTAGGCTTGTTGGGTATTGTGAGGAGAAGGAGGAGAGGCTGTTGGTTTATGAGTACATGAAGAATGGTGCGCTCCATGATCATTTACATGACAAGAACAACGTCGAGAAGAGTAGTAGTTTGATTAATTCTTGGAAAATGAGGATCAAGGTGGCTTTAGATGCTGCCAGAGGCATTGAGTATCTACATAACTATGCAGTCCCTTCTATAATTCACAGAGATATAAAGTCCTCTAACATTCTGATAGACGGAAATTGGACTGGAAGAGTATCTGATTTCGGATTATCACTATTGGGTCCGGAAAATGACAGCGACTATAGGCCAATGAAGGCAGCTGGAACCGTTGGCTATATTGATCCAGAGTACTATGGTTTGAACTTATTAACAGCAAAGAGTGATGTGTATGGACTTGGTGTGGTGTTGTTGGAGCTTCTGACAGGAAGAGCAATCTTCAGGAACAGTGAAGACGGAGGGACACCTACCAGTGTTGTGGACTTCACTGTGCCGCTGATCATGGCCGGAGAGTTAGATAAGGTTTTGGACCCAAGGGTTGGACCTCCGGAAATCAATGAAGCAGAGGCGGTCGAGTTGGTGGCGTATACTGCAATGCATTGTGTGAATTTGGAAGGTAAAGATAGACCAACCATGACTGACATTGTGGCCAATTTGGAGCGAGCATTGAGTCTCTGTGAGGAAAGCCCCGGTGGCAGCATATCTAGCGGCTCAATCATCTCTGCCGATTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr RCC1_2

Protein Analysis

791

Amino Acids

84.89

Weight (kDa)

6.49

Isoelectric Point (pI)

43.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_RLD	PF25390	104 - 301	5.7e-06	RCC1-like domain
Pkinase	PF00069	483 - 763	2e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	484 - 764	2.2e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55950
fragaria_vesca	FvH4_3g38710 FvH4_7g24490
malus_domestica	MD00G1166500.v1.1 MD01G1153100.v1.1 MD03G1068800.v1.1 MD07G1222100.v1.1 MD11G1073300.v1.1
prunus_persica	Prupe.2G250300_v2.0.a1 Prupe.6G056600_v2.0.a1
pyrus_communis	pycom01g17070 pycom03g05510
rosa_chinensis	RchiOBHm_Chr1g0370251 RchiOBHm_Chr5g0065501 RchiOBHm_Chr5g0069861
rosa_laevigata	RLG00000027065 RLG00000036103
rosa_multiflora	Rmu_co8321277.1_g000001 Rmu_sc0000738.1_g000002 Rmu_sc0026893.1_g000001 Rmu_sc0037931.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011190 Rroxscaffold_4G00286710 Rroxscaffold_4G00287300
rosa_rugosa	Rorug01G0355200 Rorug05G0400300
rosa_samantha	Rh1AG363800 Rh1BG326900 Rh1CG340200 Rh1DG357300 Rh5AG457400 Rh5BG476200 Rh5CG500000 Rh5DG488000
rosa_wichuraiana	Rw1G032010 Rw5G042700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	605
AatII	GACGTC	1 cut(s)	824
Acc36I	ACCTGC	1 cut(s)	605
AccB1I	GGYRCC	3 cut(s)	95, 125, 410
AccB7I	CCANNNNNTGG	1 cut(s)	2180
AccBSI	CCGCTC	1 cut(s)	525
AccI	GTMKAC	3 cut(s)	333, 967, 2228
AccII	CGCG	1 cut(s)	1515
AccIII	TCCGGA	2 cut(s)	1927, 2188
AclI	AACGTT	1 cut(s)	443
AclWI	GGATC	4 cut(s)	749, 1358, 1781, 1979
AcoI	YGGCCR	6 cut(s)	148, 235, 564, 594, 2145, 2289
AcsI	RAATTY	2 cut(s)	1060, 2248
AcuI	CTGAAG	2 cut(s)	798, 2065
AcvI	CACGTG	1 cut(s)	628
AcyI	GRCGYC	4 cut(s)	96, 126, 411, 821
AfaI	GTAC	4 cut(s)	1490, 1562, 1685, 1994
AfiI	CCNNNNNNNGG	9 cut(s)	237, 529, 541, 1287, 1332, 1335, 1396, 1570, 2180
AflIII	ACRYGT	1 cut(s)	627
AgsI	TTSAA	8 cut(s)	353, 449, 575, 611, 691, 926, 1129, 2005
AjnI	CCWGG	2 cut(s)	901, 955
AjuI	GAANNNNNNNTTGG	2 cut(s)	567, 599
AleI	CACNNNNGTG	2 cut(s)	630, 741
Alw21I	GWGCWC	1 cut(s)	1346
Alw26I	GTCTC	2 cut(s)	148, 2318
AlwI	GGATC	4 cut(s)	749, 1358, 1781, 1979
Ama87I	CYCGRG	2 cut(s)	1119, 1345
Aor13HI	TCCGGA	2 cut(s)	1927, 2188
ApeKI	GCWGC	7 cut(s)	160, 1275, 1427, 1430, 1793, 1961, 2337
ApoI	RAATTY	2 cut(s)	1060, 2248
ArsI	GACNNNNNNTTYG	2 cut(s)	299, 331
AseI	ATTAAT	1 cut(s)	1755
Asp700I	GAANNNNTTC	1 cut(s)	709
AspLEI	GCGC	7 cut(s)	98, 128, 165, 392, 413, 1515, 1702
AsuC2I	CCSGG	5 cut(s)	702, 844, 1120, 1121, 2331
AsuHPI	GGTGA	7 cut(s)	43, 55, 844, 1077, 1089, 1255, 1564
AsuII	TTCGAA	2 cut(s)	515, 1058
AvaI	CYCGRG	2 cut(s)	1119, 1345
AvaII	GGWCC	9 cut(s)	240, 261, 466, 477, 532, 899, 1925, 2170, 2183
BalI	TGGCCA	1 cut(s)	2291
BanI	GGYRCC	3 cut(s)	95, 125, 410
BanII	GRGCYC	1 cut(s)	1346
BbrPI	CACGTG	1 cut(s)	628
BbsI	GAAGAC	1 cut(s)	2100
Bbv12I	GWGCWC	1 cut(s)	1346
BbvCI	CCTCAGC	1 cut(s)	1401
BbvI	GCAGC	7 cut(s)	172, 1287, 1439, 1442, 1780, 1973, 2349
BccI	CCATC	6 cut(s)	41, 200, 463, 488, 1123, 1204
BceAI	ACGGC	5 cut(s)	92, 113, 353, 398, 609
BciT130I	CCWGG	2 cut(s)	903, 957
BciVI	GTATCC	2 cut(s)	263, 1567
BclI	TGATCA	2 cut(s)	1708, 2139
BcnI	CCSGG	5 cut(s)	702, 844, 1120, 1121, 2331
BcoDI	GTCTC	2 cut(s)	148, 2318
BfaI	CTAG	4 cut(s)	719, 912, 2346, 2374
BfmI	CTRYAG	2 cut(s)	1164, 1945
BfoI	RGCGCY	3 cut(s)	99, 129, 414
BfuAI	ACCTGC	1 cut(s)	605
BfuI	GTATCC	2 cut(s)	263, 1567
BglI	GCCNNNNNGGC	1 cut(s)	2334
BlpI	GCTNAGC	1 cut(s)	1419
BmcAI	AGTACT	1 cut(s)	1994
Bme1390I	CCNGG	7 cut(s)	702, 844, 903, 957, 1120, 1121, 2331
Bme18I	GGWCC	9 cut(s)	240, 261, 466, 477, 532, 899, 1925, 2170, 2183
BmeT110I	CYCGRG	2 cut(s)	1119, 1345
BmrFI	CCNGG	7 cut(s)	702, 844, 903, 957, 1120, 1121, 2331
BmrI	ACTGGG	1 cut(s)	657
BmsI	GCATC	1 cut(s)	1780
BmuI	ACTGGG	1 cut(s)	657
BoxI	GACNNNNGTC	1 cut(s)	681
BpiI	GAAGAC	1 cut(s)	2100
BplI	GAGNNNNNCTC	2 cut(s)	1394, 1426
Bpu10I	CCTNAGC	1 cut(s)	1401
Bpu1102I	GCTNAGC	1 cut(s)	1419
Bpu14I	TTCGAA	2 cut(s)	515, 1058
BpuEI	CTTGAG	2 cut(s)	511, 956
BpuMI	CCSGG	5 cut(s)	702, 844, 1120, 1121, 2331
BsaAI	YACGTR	1 cut(s)	628
BsaHI	GRCGYC	4 cut(s)	96, 126, 411, 821
BsaI	GGTCTC	1 cut(s)	148
BsaJI	CCNNGG	8 cut(s)	396, 558, 597, 1119, 1285, 1548, 2174, 2329
BsaWI	WCCGGW	4 cut(s)	534, 713, 1927, 2188
BsaXI	ACNNNNNCTCC	4 cut(s)	627, 657, 1655, 1685
Bsc4I	CCNNNNNNNGG	9 cut(s)	237, 529, 541, 1287, 1332, 1335, 1396, 1570, 2180
Bse118I	RCCGGY	2 cut(s)	122, 177
Bse1I	ACTGG	7 cut(s)	567, 652, 763, 793, 1419, 1894, 2112
Bse3DI	GCAATG	1 cut(s)	2241
BseAI	TCCGGA	2 cut(s)	1927, 2188
BseBI	CCWGG	2 cut(s)	903, 957
BseDI	CCNNGG	8 cut(s)	396, 558, 597, 1119, 1285, 1548, 2174, 2329
BseGI	GGATG	5 cut(s)	33, 1115, 1215, 1322, 1510
BseLI	CCNNNNNNNGG	9 cut(s)	237, 529, 541, 1287, 1332, 1335, 1396, 1570, 2180
BseMI	GCAATG	1 cut(s)	2241
BseMII	CTCAG	3 cut(s)	1020, 1392, 1410
BseNI	ACTGG	7 cut(s)	567, 652, 763, 793, 1419, 1894, 2112
BseRI	GAGGAG	5 cut(s)	1381, 1418, 1548, 1667, 1676
BseX3I	CGGCCG	1 cut(s)	235
BseXI	GCAGC	7 cut(s)	172, 1287, 1439, 1442, 1780, 1973, 2349
BseYI	CCCAGC	1 cut(s)	540
BsgI	GTGCAG	1 cut(s)	1488
Bsh1236I	CGCG	1 cut(s)	1515
Bsh1285I	CGRYCG	2 cut(s)	238, 2214
BshNI	GGYRCC	3 cut(s)	95, 125, 410
BsiEI	CGRYCG	2 cut(s)	238, 2214
BsiHKAI	GWGCWC	1 cut(s)	1346
BsiHKCI	CYCGRG	2 cut(s)	1119, 1345
BslFI	GGGAC	3 cut(s)	559, 1814, 2116
BslI	CCNNNNNNNGG	9 cut(s)	237, 529, 541, 1287, 1332, 1335, 1396, 1570, 2180
BsmAI	GTCTC	2 cut(s)	148, 2318
BsmFI	GGGAC	3 cut(s)	559, 1814, 2116
BsmI	GAATGC	1 cut(s)	697
Bso31I	GGTCTC	1 cut(s)	148
BsoBI	CYCGRG	2 cut(s)	1119, 1345
Bsp119I	TTCGAA	2 cut(s)	515, 1058
Bsp1286I	GDGCHC	1 cut(s)	1346
Bsp13I	TCCGGA	2 cut(s)	1927, 2188
Bsp1407I	TGTACA	1 cut(s)	1488
Bsp143I	GATC	8 cut(s)	754, 834, 1319, 1350, 1708, 1773, 1984, 2139
Bsp1720I	GCTNAGC	1 cut(s)	1419
Bsp19I	CCATGG	2 cut(s)	558, 597
BspCNI	CTCAG	3 cut(s)	1021, 1393, 1411
BspEI	TCCGGA	2 cut(s)	1927, 2188
BspFNI	CGCG	1 cut(s)	1515
BspHI	TCATGA	1 cut(s)	1353
BspMI	ACCTGC	1 cut(s)	605
BspPI	GGATC	4 cut(s)	749, 1358, 1781, 1979
BspQI	GCTCTTC	1 cut(s)	2065
BspT104I	TTCGAA	2 cut(s)	515, 1058
BspT107I	GGYRCC	3 cut(s)	95, 125, 410
BspTNI	GGTCTC	1 cut(s)	148
BsrBI	CCGCTC	1 cut(s)	525
BsrDI	GCAATG	1 cut(s)	2241
BsrFI	RCCGGY	2 cut(s)	122, 177
BsrGI	TGTACA	1 cut(s)	1488
BsrI	ACTGG	7 cut(s)	567, 652, 763, 793, 1419, 1894, 2112
BssAI	RCCGGY	2 cut(s)	122, 177
BssECI	CCNNGG	8 cut(s)	396, 558, 597, 1119, 1285, 1548, 2174, 2329
BssMI	GATC	8 cut(s)	754, 834, 1319, 1350, 1708, 1773, 1984, 2139
BssNAI	GTATAC	2 cut(s)	968, 2229
BssNI	GRCGYC	4 cut(s)	96, 126, 411, 821
BssT1I	CCWWGG	4 cut(s)	558, 597, 1548, 2174
Bst1107I	GTATAC	2 cut(s)	968, 2229
Bst2UI	CCWGG	2 cut(s)	903, 957
Bst4CI	ACNGT	6 cut(s)	397, 1083, 1089, 1972, 2090, 2131
Bst6I	CTCTTC	4 cut(s)	305, 1733, 1888, 2065
BstACI	GRCGYC	4 cut(s)	96, 126, 411, 821
BstAPI	GCANNNNNTGC	1 cut(s)	171
BstAUI	TGTACA	1 cut(s)	1488
BstBAI	YACGTR	1 cut(s)	628
BstBI	TTCGAA	2 cut(s)	515, 1058
BstC8I	GCNNGC	4 cut(s)	1043, 1273, 1425, 2304
BstDEI	CTNAG	3 cut(s)	1029, 1401, 1419
BstDSI	CCRYGG	4 cut(s)	396, 558, 597, 1285
BstF5I	GGATG	5 cut(s)	33, 1115, 1215, 1322, 1510
BstFNI	CGCG	1 cut(s)	1515
BstH2I	RGCGCY	3 cut(s)	99, 129, 414
BstHHI	GCGC	7 cut(s)	98, 128, 165, 392, 413, 1515, 1702
BstKTI	GATC	8 cut(s)	757, 837, 1322, 1353, 1711, 1776, 1987, 2142
BstMAI	GTCTC	2 cut(s)	148, 2318
BstMBI	GATC	8 cut(s)	754, 834, 1319, 1350, 1708, 1773, 1984, 2139
BstMCI	CGRYCG	2 cut(s)	238, 2214
BstNI	CCWGG	2 cut(s)	903, 957
BstPAI	GACNNNNGTC	1 cut(s)	681
BstSCI	CCNGG	7 cut(s)	700, 842, 901, 955, 1118, 1119, 2329
BstSFI	CTRYAG	2 cut(s)	1164, 1945
BstUI	CGCG	1 cut(s)	1515
BstV1I	GCAGC	7 cut(s)	172, 1287, 1439, 1442, 1780, 1973, 2349
BstV2I	GAAGAC	1 cut(s)	2100
BstX2I	RGATCY	1 cut(s)	754
BstXI	CCANNNNNNTGG	1 cut(s)	2119
BstYI	RGATCY	1 cut(s)	754
BstZ17I	GTATAC	2 cut(s)	968, 2229
BstZI	CGGCCG	1 cut(s)	235
BsuI	GTATCC	2 cut(s)	263, 1567
BtgI	CCRYGG	4 cut(s)	396, 558, 597, 1285
BtsCI	GGATG	5 cut(s)	33, 1115, 1215, 1322, 1510
BtsI	GCAGTG	1 cut(s)	275
BtsIMutI	CAGTG	5 cut(s)	275, 1412, 2095, 2119, 2127
BveI	ACCTGC	1 cut(s)	605
Cac8I	GCNNGC	4 cut(s)	1043, 1273, 1425, 2304
CciI	TCATGA	1 cut(s)	1353
CfoI	GCGC	7 cut(s)	98, 128, 165, 392, 413, 1515, 1702
Cfr10I	RCCGGY	2 cut(s)	122, 177
Cfr9I	CCCGGG	1 cut(s)	1119
CpoI	CGGWCCG	1 cut(s)	261
Csp6I	GTAC	4 cut(s)	1489, 1561, 1684, 1993
CspI	CGGWCCG	1 cut(s)	261
CviAII	CATG	9 cut(s)	80, 559, 598, 1354, 1687, 1706, 1718, 2143, 2275
CviQI	GTAC	4 cut(s)	1489, 1561, 1684, 1993
DdeI	CTNAG	3 cut(s)	1029, 1401, 1419
DinI	GGCGCC	3 cut(s)	97, 127, 412
DpnI	GATC	8 cut(s)	756, 836, 1321, 1352, 1710, 1775, 1986, 2141
DpnII	GATC	8 cut(s)	754, 834, 1319, 1350, 1708, 1773, 1984, 2139
EaeI	YGGCCR	6 cut(s)	148, 235, 564, 594, 2145, 2289
EagI	CGGCCG	1 cut(s)	235
Eam1104I	CTCTTC	4 cut(s)	305, 1733, 1888, 2065
EarI	CTCTTC	4 cut(s)	305, 1733, 1888, 2065
EciI	GGCGGA	1 cut(s)	282
Ecl136II	GAGCTC	1 cut(s)	1344
EclXI	CGGCCG	1 cut(s)	235
Eco130I	CCWWGG	4 cut(s)	558, 597, 1548, 2174
Eco147I	AGGCCT	1 cut(s)	974
Eco24I	GRGCYC	1 cut(s)	1346
Eco31I	GGTCTC	1 cut(s)	148
Eco47I	GGWCC	9 cut(s)	240, 261, 466, 477, 532, 899, 1925, 2170, 2183
Eco52I	CGGCCG	1 cut(s)	235
Eco53kI	GAGCTC	1 cut(s)	1344
Eco57I	CTGAAG	2 cut(s)	798, 2065
Eco72I	CACGTG	1 cut(s)	628
Eco88I	CYCGRG	2 cut(s)	1119, 1345
EcoICRI	GAGCTC	1 cut(s)	1344
EcoO109I	RGGNCCY	1 cut(s)	899
EcoRII	CCWGG	2 cut(s)	901, 955
EcoT14I	CCWWGG	4 cut(s)	558, 597, 1548, 2174
EcoT22I	ATGCAT	1 cut(s)	2241
EcoT38I	GRGCYC	1 cut(s)	1346
EgeI	GGCGCC	3 cut(s)	97, 127, 412
EheI	GGCGCC	3 cut(s)	97, 127, 412
ErhI	CCWWGG	4 cut(s)	558, 597, 1548, 2174
FaeI	CATG	9 cut(s)	83, 562, 601, 1357, 1690, 1709, 1721, 2146, 2278
FalI	AAGNNNNNCTT	2 cut(s)	918, 950
FaqI	GGGAC	3 cut(s)	559, 1814, 2116
FatI	CATG	9 cut(s)	79, 558, 597, 1353, 1686, 1705, 1717, 2142, 2274
FbaI	TGATCA	2 cut(s)	1708, 2139
FblI	GTMKAC	3 cut(s)	333, 967, 2228
FokI	GGATG	5 cut(s)	20, 1102, 1222, 1309, 1517
FriOI	GRGCYC	1 cut(s)	1346
FspBI	CTAG	4 cut(s)	719, 912, 2346, 2374
GlaI	GCGC	7 cut(s)	97, 127, 164, 391, 412, 1514, 1701
GsaI	CCCAGC	1 cut(s)	544
HaeII	RGCGCY	3 cut(s)	99, 129, 414
HhaI	GCGC	7 cut(s)	98, 128, 165, 392, 413, 1515, 1702
Hin1I	GRCGYC	4 cut(s)	96, 126, 411, 821
Hin1II	CATG	9 cut(s)	83, 562, 601, 1357, 1690, 1709, 1721, 2146, 2278
Hin6I	GCGC	7 cut(s)	96, 126, 163, 390, 411, 1513, 1700
HinP1I	GCGC	7 cut(s)	96, 126, 163, 390, 411, 1513, 1700
HindIII	AAGCTT	1 cut(s)	932
HinfI	GANTC	8 cut(s)	308, 330, 636, 677, 949, 1109, 1586, 2311
HphI	GGTGA	7 cut(s)	43, 55, 844, 1077, 1089, 1255, 1564
Hpy166II	GTNNAC	6 cut(s)	63, 334, 968, 1489, 2122, 2229
Hpy188III	TCNNGA	8 cut(s)	1317, 1354, 1528, 1735, 1928, 1988, 2083, 2189
Hpy8I	GTNNAC	6 cut(s)	63, 334, 968, 1489, 2122, 2229
Hpy99I	CGWCG	6 cut(s)	374, 823, 826, 1093, 1384, 1736
HpyCH4III	ACNGT	6 cut(s)	397, 1083, 1089, 1972, 2090, 2131
HpyCH4IV	ACGT	4 cut(s)	443, 627, 821, 1731
HpyF3I	CTNAG	3 cut(s)	1029, 1401, 1419
HpySE526I	ACGT	4 cut(s)	443, 627, 821, 1731
Hsp92I	GRCGYC	4 cut(s)	96, 126, 411, 821
Hsp92II	CATG	9 cut(s)	83, 562, 601, 1357, 1690, 1709, 1721, 2146, 2278
HspAI	GCGC	7 cut(s)	96, 126, 163, 390, 411, 1513, 1700
KasI	GGCGCC	3 cut(s)	95, 125, 410
Kpn2I	TCCGGA	2 cut(s)	1927, 2188
Ksp22I	TGATCA	2 cut(s)	1708, 2139
Kzo9I	GATC	8 cut(s)	754, 834, 1319, 1350, 1708, 1773, 1984, 2139
LguI	GCTCTTC	1 cut(s)	2065
LmnI	GCTCC	7 cut(s)	95, 728, 1283, 1473, 1707, 2056, 2299
Lsp1109I	GCAGC	7 cut(s)	172, 1287, 1439, 1442, 1780, 1973, 2349
LweI	GCATC	1 cut(s)	1780
MaeI	CTAG	4 cut(s)	719, 912, 2346, 2374
MaeII	ACGT	4 cut(s)	443, 627, 821, 1731
MaeIII	GTNAC	4 cut(s)	284, 643, 808, 1083
MalI	GATC	8 cut(s)	756, 836, 1321, 1352, 1710, 1775, 1986, 2141
MbiI	CCGCTC	1 cut(s)	525
MboI	GATC	8 cut(s)	754, 834, 1319, 1350, 1708, 1773, 1984, 2139
MfeI	CAATTG	1 cut(s)	686
MflI	RGATCY	1 cut(s)	754
MhlI	GDGCHC	1 cut(s)	1346
MlsI	TGGCCA	1 cut(s)	2291
MluNI	TGGCCA	1 cut(s)	2291
Mly113I	GGCGCC	3 cut(s)	96, 126, 411
MlyI	GAGTC	6 cut(s)	302, 339, 645, 671, 1580, 2320
MmeI	TCCRAC	5 cut(s)	352, 444, 1305, 2034, 2161
Mox20I	TGGCCA	1 cut(s)	2291
Mph1103I	ATGCAT	1 cut(s)	2241
MroI	TCCGGA	2 cut(s)	1927, 2188
MroXI	GAANNNNTTC	1 cut(s)	709
MscI	TGGCCA	1 cut(s)	2291
MseI	TTAA	4 cut(s)	339, 866, 1755, 2012
MslI	CAYNNNNRTG	4 cut(s)	630, 741, 1716, 1821
Msp20I	TGGCCA	1 cut(s)	2291
MspA1I	CMGCKG	3 cut(s)	741, 1964, 2137
MspR9I	CCNGG	7 cut(s)	702, 844, 903, 957, 1120, 1121, 2331
MunI	CAATTG	1 cut(s)	686
Mva1269I	GAATGC	1 cut(s)	697
MvaI	CCWGG	2 cut(s)	903, 957
MvnI	CGCG	1 cut(s)	1515
NarI	GGCGCC	3 cut(s)	96, 126, 411
NciI	CCSGG	5 cut(s)	702, 844, 1120, 1121, 2331
NcoI	CCATGG	2 cut(s)	558, 597
NdeII	GATC	8 cut(s)	754, 834, 1319, 1350, 1708, 1773, 1984, 2139
NlaIII	CATG	9 cut(s)	83, 562, 601, 1357, 1690, 1709, 1721, 2146, 2278
NmeAIII	GCCGAG	1 cut(s)	66
NmuCI	GTSAC	2 cut(s)	808, 1083
NsiI	ATGCAT	1 cut(s)	2241
NspV	TTCGAA	2 cut(s)	515, 1058
OliI	CACNNNNGTG	2 cut(s)	630, 741
PaeR7I	CTCGAG	1 cut(s)	1345
PagI	TCATGA	1 cut(s)	1353
PaqCI	CACCTGC	1 cut(s)	605
PceI	AGGCCT	1 cut(s)	974
PciSI	GCTCTTC	1 cut(s)	2065
PcsI	WCGNNNNNNNCGW	1 cut(s)	599
PctI	GAATGC	1 cut(s)	697
PdmI	GAANNNNTTC	1 cut(s)	709
PfeI	GAWTC	2 cut(s)	949, 1109
PflMI	CCANNNNNTGG	1 cut(s)	2180
PleI	GAGTC	6 cut(s)	302, 338, 644, 671, 1580, 2319
PluTI	GGCGCC	3 cut(s)	99, 129, 414
PmaCI	CACGTG	1 cut(s)	628
PmlI	CACGTG	1 cut(s)	628
PpsI	GAGTC	6 cut(s)	302, 338, 644, 671, 1580, 2319
Ppu21I	YACGTR	1 cut(s)	628
PpuMI	RGGWCCY	1 cut(s)	899
PshAI	GACNNNNGTC	1 cut(s)	681
PshBI	ATTAAT	1 cut(s)	1755
Psp124BI	GAGCTC	1 cut(s)	1346
Psp1406I	AACGTT	1 cut(s)	443
Psp5II	RGGWCCY	1 cut(s)	899
Psp6I	CCWGG	2 cut(s)	901, 955
PspCI	CACGTG	1 cut(s)	628
PspFI	CCCAGC	1 cut(s)	540
PspGI	CCWGG	2 cut(s)	901, 955
PspPPI	RGGWCCY	1 cut(s)	899
PspXI	VCTCGAGB	1 cut(s)	1345
PsuI	RGATCY	1 cut(s)	754
PvuII	CAGCTG	2 cut(s)	741, 1964
RsaI	GTAC	4 cut(s)	1490, 1562, 1685, 1994
RsaNI	GTAC	4 cut(s)	1489, 1561, 1684, 1993
RseI	CAYNNNNRTG	4 cut(s)	630, 741, 1716, 1821
Rsr2I	CGGWCCG	1 cut(s)	261
RsrII	CGGWCCG	1 cut(s)	261
SacI	GAGCTC	1 cut(s)	1346
SapI	GCTCTTC	1 cut(s)	2065
SaqAI	TTAA	4 cut(s)	339, 866, 1755, 2012
Sau3AI	GATC	8 cut(s)	754, 834, 1319, 1350, 1708, 1773, 1984, 2139
ScaI	AGTACT	1 cut(s)	1994
SchI	GAGTC	6 cut(s)	302, 339, 645, 671, 1580, 2320
ScrFI	CCNGG	7 cut(s)	702, 844, 903, 957, 1120, 1121, 2331
SduI	GDGCHC	1 cut(s)	1346
SfaNI	GCATC	1 cut(s)	1780
SfcI	CTRYAG	2 cut(s)	1164, 1945
SfoI	GGCGCC	3 cut(s)	97, 127, 412
Sfr274I	CTCGAG	1 cut(s)	1345
SfuI	TTCGAA	2 cut(s)	515, 1058
SgrAI	CRCCGGYG	1 cut(s)	122
SinI	GGWCC	9 cut(s)	240, 261, 466, 477, 532, 899, 1925, 2170, 2183
SlaI	CTCGAG	1 cut(s)	1345
SmaI	CCCGGG	1 cut(s)	1121
SmiMI	CAYNNNNRTG	4 cut(s)	630, 741, 1716, 1821
SmlI	CTYRAG	3 cut(s)	526, 935, 1345
SmoI	CTYRAG	3 cut(s)	526, 935, 1345
SseBI	AGGCCT	1 cut(s)	974
SspDI	GGCGCC	3 cut(s)	95, 125, 410
SspMI	CTAG	4 cut(s)	719, 912, 2346, 2374
SstI	GAGCTC	1 cut(s)	1346
StuI	AGGCCT	1 cut(s)	974
StyD4I	CCNGG	7 cut(s)	700, 842, 901, 955, 1118, 1119, 2329
StyI	CCWWGG	4 cut(s)	558, 597, 1548, 2174
TaaI	ACNGT	6 cut(s)	397, 1083, 1089, 1972, 2090, 2131
TaiI	ACGT	4 cut(s)	446, 630, 824, 1734
TaqII	GACCGA	1 cut(s)	249
TatI	WGTACW	3 cut(s)	1488, 1683, 1992
TauI	GCSGC	8 cut(s)	138, 237, 255, 566, 1339, 1363, 2137, 2352
TfiI	GAWTC	2 cut(s)	949, 1109
Tru1I	TTAA	4 cut(s)	339, 866, 1755, 2012
Tru9I	TTAA	4 cut(s)	339, 866, 1755, 2012
TscAI	CASTG	5 cut(s)	282, 1419, 2095, 2119, 2134
TseFI	GTSAC	2 cut(s)	808, 1083
TseI	GCWGC	7 cut(s)	160, 1275, 1427, 1430, 1793, 1961, 2337
Tsp45I	GTSAC	2 cut(s)	808, 1083
TspDTI	ATGAA	7 cut(s)	34, 357, 1229, 1604, 1703, 1970, 2214
TspGWI	ACGGA	3 cut(s)	187, 1893, 2112
TspMI	CCCGGG	1 cut(s)	1119
TspRI	CASTG	5 cut(s)	282, 1419, 2095, 2119, 2134
Van91I	CCANNNNNTGG	1 cut(s)	2180
VpaK11BI	GGWCC	9 cut(s)	240, 261, 466, 477, 532, 899, 1925, 2170, 2183
VspI	ATTAAT	1 cut(s)	1755
XapI	RAATTY	2 cut(s)	1060, 2248
XhoI	CTCGAG	1 cut(s)	1345
XmaI	CCCGGG	1 cut(s)	1119
XmiI	GTMKAC	3 cut(s)	333, 967, 2228
XmnI	GAANNNNTTC	1 cut(s)	709
XspI	CTAG	4 cut(s)	719, 912, 2346, 2374
ZraI	GACGTC	1 cut(s)	822
ZrmI	AGTACT	1 cut(s)	1994
Zsp2I	ATGCAT	1 cut(s)	2241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.