Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003366

Physical Location & Seq

Genomic Coordinates Reverse (-)

29901654 .. 29904086

Gene Structure 2433 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1222100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2307 bp

ATGACGAAACGACCCTTCTCCTCCCTTACCACCGCCACCTTCATCATCATCACCGCCACCATCCTCTTCGCCGTTATACCACCACAAGCTCATGCCTTCGGTTCCGGCTCCACCCTTGCCGTTATCTCCGCCTCCGGCACCGTCTGCGGTGTCGTGTCAGACCAACCGCGGCAGCGCATCGTCTGCTACCGCCGCGGACAAACCGTCGCAGTCGAGCCCAACATCTCCTTCTCCGCCATCTCCGGCGGACGGACCTCTCTATGCGGCCTCCGCTCTGGCGGATACAGCATCCTCTGCTGGGACGACATCGCCTCCCAAAACTCCACCTTCCCATCCAAACGTCTTTACTTCAACAAAACCGTTACGTTAGAAAATCTCTCAGTCGGCGACGACCATATCTGCGCCAGAACCGTCGTCACAGGCACCATAAAATGCTGGCGGGTCGACGACGTGTCCGAATTACCATCTGGGTCGGACCAATTCTCCTCGATTTCATCGGGCAACTTCAATGGCGGAAGAAAATACAAAACCGGGTTCGGAAACATGACAATGGCGACCATCACAGCCGGCGGCTCGCACGTGTGCGGGATCAACACCACAGGACTTATAGTCTGCCGGGGAAGCAATGGCGCCGGCCAATGGAACGTCCCTCCTGGCTCGCCTTTCGAGTTCTCTGCTCTGTCCCTCGGAGTAAACCACAGCTGCGCAATCAGAACTTCAAATAAAACGGTCATTTGTTGGGGAGGAGGAGGTCAATTTGCATTTAACCAAACAAACGGGGTGTCTTTTGAAGTAATTGCTTCTGGGTCGGATTTCGTTTGTGGATTAACCACCAGGAATTTTTCGATTGTTTGTTGGGGTACGGGCTGGCCCAATCTCTCCGGTTTGAAATCAGGGGAGGAGCTCGAATTGCCCGAAATTCTTCCCGGTTCTTGTGTCAATTCCTCCTGCGGTGAATGTGGAATTTACCCTCAGTCCCAAAGGCTCTGTTTTGGTGCAGACAACATTTGCAAGCCCTGTTTGCCTCCAATTTCATTGCCACCACCAGCATTTCCACCGCCGCCGCCGTCGCCGCAGCCGCCACCGCCCCTGCCGTCAAGGCTGCCGCCCGCGGCATTGACGACGGGTTTATTGATGTTTGCCATTGTCGGTTCTGTCGGAGCTTTTGCAGTTCAGCCGACAATTACTAGAGATGCTAGCTCAAACAATGGCAATGGGATAACGTCAAACAATAGTCCCCCGTCGAGATCCTCCACCATCCGCCGCCAGGGCTCGAGGATCATGAGGCGGCAGAGGAGCGGAACATCCTCGAAGCATGCAGACAGGGCGGAGGAGTTTTCCCTGTCCGAGCTCGCGGCCGCCACCAATGACTTCTCAATGGAGAACAAGATTGGCGCCGGAAGCTTCGGAGTTGTATATAGAGGCAAACTGCCTGACGGCCGGGAAGTTGCGATCAAGAGAGGCGAAACCGCCCCGAAGATGAAGAAGTATCAGGAGAAGGAGAGCGCATTCGATTCGGAACTTGCGTTCTTGTCGAGAGTTCATCACAAACATCTCGTGAGGCTTGTCGGATATTGCCAAGAGAGGGACGAGAGGCTTTTGGTGTACGAGCACGTGAAAAACGGTGCGCTTTATGATCACTTGCATGACAAGAACAATGTGGAGAGAAGCAGTAGTTTGTTAAATTCGTGGAAAATGAGGATCAAGGTTGCATTGGATGCCTCCAGAGGCATTGAGTATCTGCACAATTACGCCGTGCCGCCTATCATTCATCGAGATATAAAGTCATCGAACATCTTGCTCGACGCGAATTGGACCGCGAGGGTGTCTGATTTTGGGTTGTCGTTGATGGGGCCGGAGTCAGAGGTGGATTTTAGGCCAACGAAGGCGGCAGGGACGGTTGGCTACATTGATCCGGAGTACTACGGACTCAACTTGTTGACCGCGAAGAGCGATGTCTATGGACTCGGCGTGGTGCTACTGGAGCTTCTGACGGGGAAGAGGGCAATATTTAAGGATGGTGAGGATGGGGGGACGCCCATAAGCGTGGTGGATTTCGCAGTGCCGGCGATTATGAAAGGGGAGTTGGTGAGGGTTTTGGACAAGAGGGTTGGGCCTCCGGAAGTGAATGAATCGGAGGCGGTTGAGCTGGTGGCGTATACAGCAATGCATTGTGTGAGTTTGGAAGGGAAAGATAGGCCGACGATGGCGGACATTGTGGCGAACTTGGAGAGGGCGTATGCCCTGTGCGATGAAAGCCCTGGCAGCATCTCAAGTGGTGCAATCTCCATTGTCTCAGCAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr RCC1_2

Protein Analysis

769

Amino Acids

82.2

Weight (kDa)

7.97

Isoelectric Point (pI)

42.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	459 - 740	5.3e-40	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	459 - 743	5.6e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55950
fragaria_vesca	FvH4_3g38710 FvH4_7g24490
malus_domestica	MD00G1166500.v1.1 MD01G1153100.v1.1 MD03G1068800.v1.1 MD07G1222100.v1.1 MD11G1073300.v1.1
prunus_persica	Prupe.2G250300_v2.0.a1 Prupe.6G056600_v2.0.a1
pyrus_communis	pycom01g17070 pycom03g05510
rosa_chinensis	RchiOBHm_Chr1g0370251 RchiOBHm_Chr5g0065501 RchiOBHm_Chr5g0069861
rosa_laevigata	RLG00000027065 RLG00000036103
rosa_multiflora	Rmu_co8321277.1_g000001 Rmu_sc0000738.1_g000002 Rmu_sc0026893.1_g000001 Rmu_sc0037931.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011190 Rroxscaffold_4G00286710 Rroxscaffold_4G00287300
rosa_rugosa	Rorug01G0355200 Rorug05G0400300
rosa_samantha	Rh1AG363800 Rh1BG326900 Rh1CG340200 Rh1DG357300 Rh5AG457400 Rh5BG476200 Rh5CG500000 Rh5DG488000
rosa_wichuraiana	Rw1G032010 Rw5G042700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	706
AccB1I	GGYRCC	4 cut(s)	137, 422, 629, 1394
AccBSI	CCGCTC	2 cut(s)	273, 1299
AccI	GTMKAC	2 cut(s)	444, 2159
AccII	CGCG	7 cut(s)	169, 195, 1112, 1355, 1808, 1820, 1946
AccIII	TCCGGA	2 cut(s)	1915, 2119
AclWI	GGATC	5 cut(s)	596, 1242, 1286, 1709, 1907
AcoI	YGGCCR	3 cut(s)	634, 1356, 1438
AcsI	RAATTY	4 cut(s)	838, 918, 963, 1684
AcvI	CACGTG	2 cut(s)	580, 1615
AcyI	GRCGYC	3 cut(s)	630, 1395, 2036
AfaI	GTAC	3 cut(s)	862, 1607, 1922
AfiI	CCNNNNNNNGG	5 cut(s)	249, 298, 639, 1267, 1585
AflIII	ACRYGT	2 cut(s)	450, 579
AgsI	TTSAA	5 cut(s)	352, 508, 720, 791, 889
AhdI	GACNNNNNGTC	3 cut(s)	203, 451, 608
AjiI	CACGTC	1 cut(s)	451
AjnI	CCWGG	4 cut(s)	652, 833, 1266, 2260
AjuI	GAANNNNNNNTTGG	4 cut(s)	212, 244, 2069, 2101
AloI	GAACNNNNNNTCC	2 cut(s)	1511, 1543
AluBI	AGCT	9 cut(s)	89, 702, 904, 1163, 1200, 1351, 1404, 1987, 2149
AluI	AGCT	9 cut(s)	89, 702, 904, 1163, 1200, 1351, 1404, 1987, 2149
Alw21I	GWGCWC	3 cut(s)	906, 1353, 1614
Alw26I	GTCTC	1 cut(s)	2299
AlwI	GGATC	5 cut(s)	596, 1242, 1286, 1709, 1907
Ama87I	CYCGRG	1 cut(s)	1273
Aor13HI	TCCGGA	2 cut(s)	1915, 2119
AoxI	GGCC	9 cut(s)	265, 634, 869, 1356, 1438, 1853, 1877, 2114, 2198
ApeKI	GCWGC	5 cut(s)	172, 702, 1075, 1102, 2265
ApoI	RAATTY	4 cut(s)	838, 918, 963, 1684
ArsI	GACNNNNNNTTYG	2 cut(s)	767, 799
Asp700I	GAANNNNTTC	1 cut(s)	921
AspLEI	GCGC	7 cut(s)	177, 404, 632, 707, 1397, 1508, 1630
AspS9I	GGNCC	6 cut(s)	252, 475, 870, 1815, 1853, 2114
AsuC2I	CCSGG	4 cut(s)	532, 617, 927, 1442
AsuHPI	GGTGA	4 cut(s)	43, 965, 2033, 2101
AsuNHI	GCTAGC	1 cut(s)	1196
AvaI	CYCGRG	1 cut(s)	1273
AvaII	GGWCC	3 cut(s)	252, 475, 1815
BanI	GGYRCC	4 cut(s)	137, 422, 629, 1394
BanII	GRGCYC	4 cut(s)	219, 906, 1274, 1353
BauI	CACGAG	1 cut(s)	1556
BbrPI	CACGTG	2 cut(s)	580, 1615
Bbv12I	GWGCWC	3 cut(s)	906, 1353, 1614
BbvI	GCAGC	5 cut(s)	184, 689, 1087, 1089, 2277
BceAI	ACGGC	6 cut(s)	56, 104, 1051, 1078, 1453, 1739
BcgI	CGANNNNNNTGC	6 cut(s)	656, 690, 1497, 1531, 1780, 1814
BciT130I	CCWGG	4 cut(s)	654, 835, 1268, 2262
BciVI	GTATCC	1 cut(s)	275
BclI	TGATCA	1 cut(s)	1636
BcnI	CCSGG	4 cut(s)	532, 617, 927, 1442
BcoDI	GTCTC	1 cut(s)	2299
BfaI	CTAG	2 cut(s)	1188, 1197
BfoI	RGCGCY	2 cut(s)	633, 1398
BfuI	GTATCC	1 cut(s)	275
BglI	GCCNNNNNGGC	2 cut(s)	1099, 1269
BmcAI	AGTACT	1 cut(s)	1922
Bme1390I	CCNGG	8 cut(s)	532, 617, 654, 835, 927, 1268, 1442, 2262
Bme18I	GGWCC	3 cut(s)	252, 475, 1815
BmeRI	GACNNNNNGTC	3 cut(s)	203, 451, 608
BmeT110I	CYCGRG	1 cut(s)	1273
BmgBI	CACGTC	1 cut(s)	451
BmgT120I	GGNCC	6 cut(s)	252, 475, 870, 1815, 1853, 2114
BmiI	GGNNCC	7 cut(s)	103, 109, 139, 424, 631, 1396, 1854
BmrFI	CCNGG	8 cut(s)	532, 617, 654, 835, 927, 1268, 1442, 2262
BmsI	GCATC	5 cut(s)	186, 297, 1183, 1708, 2277
BmtI	GCTAGC	1 cut(s)	1200
BpmI	CTGGAG	2 cut(s)	1708, 2003
BpuEI	CTTGAG	1 cut(s)	2257
BpuMI	CCSGG	4 cut(s)	532, 617, 927, 1442
BsaAI	YACGTR	2 cut(s)	580, 1615
BsaHI	GRCGYC	3 cut(s)	630, 1395, 2036
BsaJI	CCNNGG	7 cut(s)	167, 193, 616, 685, 1110, 1267, 2260
BsaWI	WCCGGW	3 cut(s)	881, 1915, 2119
BsaXI	ACNNNNNCTCC	6 cut(s)	296, 326, 467, 497, 2222, 2252
Bsc4I	CCNNNNNNNGG	5 cut(s)	249, 298, 639, 1267, 1585
Bse118I	RCCGGY	3 cut(s)	566, 632, 2065
Bse1I	ACTGG	1 cut(s)	1986
Bse3DI	GCAATG	4 cut(s)	631, 1034, 1219, 2172
BseAI	TCCGGA	2 cut(s)	1915, 2119
BseBI	CCWGG	4 cut(s)	654, 835, 1268, 2262
BseDI	CCNNGG	7 cut(s)	167, 193, 616, 685, 1110, 1267, 2260
BseGI	GGATG	8 cut(s)	60, 288, 332, 1257, 1304, 1723, 2023, 2032
BseLI	CCNNNNNNNGG	5 cut(s)	249, 298, 639, 1267, 1585
BseMI	GCAATG	4 cut(s)	631, 1034, 1219, 2172
BseMII	CTCAG	2 cut(s)	393, 986
BseNI	ACTGG	1 cut(s)	1986
BseRI	GAGGAG	7 cut(s)	10, 475, 759, 762, 914, 1309, 1346
BseX3I	CGGCCG	2 cut(s)	1356, 1438
BseXI	GCAGC	5 cut(s)	184, 689, 1087, 1089, 2277
BseYI	CCCAGC	1 cut(s)	297
BsgI	GTGCAG	2 cut(s)	1017, 1727
Bsh1236I	CGCG	7 cut(s)	169, 195, 1112, 1355, 1808, 1820, 1946
Bsh1285I	CGRYCG	2 cut(s)	1359, 1441
BshFI	GGCC	9 cut(s)	267, 636, 871, 1358, 1440, 1855, 1879, 2116, 2200
BshNI	GGYRCC	4 cut(s)	137, 422, 629, 1394
BsiEI	CGRYCG	2 cut(s)	1359, 1441
BsiHKAI	GWGCWC	3 cut(s)	906, 1353, 1614
BsiHKCI	CYCGRG	1 cut(s)	1273
BslFI	GGGAC	8 cut(s)	314, 632, 667, 961, 1221, 1601, 1909, 2047
BslI	CCNNNNNNNGG	5 cut(s)	249, 298, 639, 1267, 1585
BsmAI	GTCTC	1 cut(s)	2299
BsmFI	GGGAC	8 cut(s)	314, 632, 667, 961, 1221, 1601, 1909, 2047
BsmI	GAATGC	1 cut(s)	1508
BsnI	GGCC	9 cut(s)	267, 636, 871, 1358, 1440, 1855, 1879, 2116, 2200
BsoBI	CYCGRG	1 cut(s)	1273
Bsp1286I	GDGCHC	5 cut(s)	219, 906, 1274, 1353, 1614
Bsp13I	TCCGGA	2 cut(s)	1915, 2119
Bsp143I	GATC	7 cut(s)	588, 1247, 1278, 1452, 1636, 1701, 1912
BspANI	GGCC	9 cut(s)	267, 636, 871, 1358, 1440, 1855, 1879, 2116, 2200
BspCNI	CTCAG	2 cut(s)	392, 985
BspEI	TCCGGA	2 cut(s)	1915, 2119
BspFNI	CGCG	7 cut(s)	169, 195, 1112, 1355, 1808, 1820, 1946
BspHI	TCATGA	1 cut(s)	1281
BspLI	GGNNCC	7 cut(s)	103, 109, 139, 424, 631, 1396, 1854
BspOI	GCTAGC	1 cut(s)	1200
BspPI	GGATC	5 cut(s)	596, 1242, 1286, 1709, 1907
BspQI	GCTCTTC	1 cut(s)	1943
BspT107I	GGYRCC	4 cut(s)	137, 422, 629, 1394
BsrBI	CCGCTC	2 cut(s)	273, 1299
BsrDI	GCAATG	4 cut(s)	631, 1034, 1219, 2172
BsrFI	RCCGGY	3 cut(s)	566, 632, 2065
BsrI	ACTGG	1 cut(s)	1986
BssAI	RCCGGY	3 cut(s)	566, 632, 2065
BssECI	CCNNGG	7 cut(s)	167, 193, 616, 685, 1110, 1267, 2260
BssMI	GATC	7 cut(s)	588, 1247, 1278, 1452, 1636, 1701, 1912
BssNAI	GTATAC	1 cut(s)	2160
BssNI	GRCGYC	3 cut(s)	630, 1395, 2036
BssSI	CACGAG	1 cut(s)	1556
Bst1107I	GTATAC	1 cut(s)	2160
Bst2BI	CACGAG	1 cut(s)	1556
Bst2UI	CCWGG	4 cut(s)	654, 835, 1268, 2262
Bst4CI	ACNGT	7 cut(s)	142, 205, 361, 412, 730, 1625, 1900
Bst6I	CTCTTC	3 cut(s)	71, 1943, 1994
BstACI	GRCGYC	3 cut(s)	630, 1395, 2036
BstAPI	GCANNNNNTGC	4 cut(s)	144, 183, 294, 1718
BstBAI	YACGTR	2 cut(s)	580, 1615
BstDEI	CTNAG	3 cut(s)	379, 972, 2296
BstDSI	CCRYGG	3 cut(s)	167, 193, 1110
BstF5I	GGATG	8 cut(s)	60, 288, 332, 1257, 1304, 1723, 2023, 2032
BstFNI	CGCG	7 cut(s)	169, 195, 1112, 1355, 1808, 1820, 1946
BstH2I	RGCGCY	2 cut(s)	633, 1398
BstHHI	GCGC	7 cut(s)	177, 404, 632, 707, 1397, 1508, 1630
BstKTI	GATC	7 cut(s)	591, 1250, 1281, 1455, 1639, 1704, 1915
BstMAI	GTCTC	1 cut(s)	2299
BstMBI	GATC	7 cut(s)	588, 1247, 1278, 1452, 1636, 1701, 1912
BstMCI	CGRYCG	2 cut(s)	1359, 1441
BstNI	CCWGG	4 cut(s)	654, 835, 1268, 2262
BstNSI	RCATGY	1 cut(s)	1319
BstSCI	CCNGG	8 cut(s)	530, 615, 652, 833, 925, 1266, 1440, 2260
BstUI	CGCG	7 cut(s)	169, 195, 1112, 1355, 1808, 1820, 1946
BstV1I	GCAGC	5 cut(s)	184, 689, 1087, 1089, 2277
BstX2I	RGATCY	1 cut(s)	1247
BstXI	CCANNNNNNTGG	1 cut(s)	2047
BstYI	RGATCY	1 cut(s)	1247
BstZ17I	GTATAC	1 cut(s)	2160
BstZI	CGGCCG	2 cut(s)	1356, 1438
BsuI	GTATCC	1 cut(s)	275
BsuRI	GGCC	9 cut(s)	267, 636, 871, 1358, 1440, 1855, 1879, 2116, 2200
BtgI	CCRYGG	3 cut(s)	167, 193, 1110
BtgZI	GCGATG	3 cut(s)	292, 1968, 2265
BtrI	CACGTC	1 cut(s)	451
BtsCI	GGATG	8 cut(s)	60, 288, 332, 1257, 1304, 1723, 2023, 2032
BtsI	GCAGTG	1 cut(s)	2067
BtsIMutI	CAGTG	1 cut(s)	2067
CciI	TCATGA	1 cut(s)	1281
CciNI	GCGGCCGC	1 cut(s)	1356
CfoI	GCGC	7 cut(s)	177, 404, 632, 707, 1397, 1508, 1630
Cfr10I	RCCGGY	3 cut(s)	566, 632, 2065
Cfr13I	GGNCC	6 cut(s)	252, 475, 870, 1815, 1853, 2114
Cfr42I	CCGCGG	3 cut(s)	170, 196, 1113
CseI	GACGC	2 cut(s)	1814, 2044
Csp6I	GTAC	3 cut(s)	861, 1606, 1921
CviAII	CATG	5 cut(s)	92, 544, 1282, 1316, 1646
CviQI	GTAC	3 cut(s)	861, 1606, 1921
DdeI	CTNAG	3 cut(s)	379, 972, 2296
DinI	GGCGCC	2 cut(s)	631, 1396
DpnI	GATC	7 cut(s)	590, 1249, 1280, 1454, 1638, 1703, 1914
DpnII	GATC	7 cut(s)	588, 1247, 1278, 1452, 1636, 1701, 1912
DriI	GACNNNNNGTC	3 cut(s)	203, 451, 608
EaeI	YGGCCR	3 cut(s)	634, 1356, 1438
EagI	CGGCCG	2 cut(s)	1356, 1438
Eam1104I	CTCTTC	3 cut(s)	71, 1943, 1994
Eam1105I	GACNNNNNGTC	3 cut(s)	203, 451, 608
EarI	CTCTTC	3 cut(s)	71, 1943, 1994
EciI	GGCGGA	8 cut(s)	118, 223, 261, 294, 528, 1250, 1343, 2225
Ecl136II	GAGCTC	2 cut(s)	904, 1351
EclXI	CGGCCG	2 cut(s)	1356, 1438
Eco24I	GRGCYC	4 cut(s)	219, 906, 1274, 1353
Eco47I	GGWCC	3 cut(s)	252, 475, 1815
Eco52I	CGGCCG	2 cut(s)	1356, 1438
Eco53kI	GAGCTC	2 cut(s)	904, 1351
Eco72I	CACGTG	2 cut(s)	580, 1615
Eco88I	CYCGRG	1 cut(s)	1273
EcoICRI	GAGCTC	2 cut(s)	904, 1351
EcoRII	CCWGG	4 cut(s)	652, 833, 1266, 2260
EcoT22I	ATGCAT	1 cut(s)	2172
EcoT38I	GRGCYC	4 cut(s)	219, 906, 1274, 1353
EgeI	GGCGCC	2 cut(s)	631, 1396
EheI	GGCGCC	2 cut(s)	631, 1396
FaeI	CATG	5 cut(s)	95, 547, 1285, 1319, 1649
FaqI	GGGAC	8 cut(s)	314, 632, 667, 961, 1221, 1601, 1909, 2047
FatI	CATG	5 cut(s)	91, 543, 1281, 1315, 1645
FauI	CCCGC	3 cut(s)	432, 578, 1117
FbaI	TGATCA	1 cut(s)	1636
FblI	GTMKAC	2 cut(s)	444, 2159
FokI	GGATG	8 cut(s)	47, 275, 319, 1244, 1291, 1730, 2030, 2039
FriOI	GRGCYC	4 cut(s)	219, 906, 1274, 1353
FspBI	CTAG	2 cut(s)	1188, 1197
FspI	TGCGCA	1 cut(s)	706
GlaI	GCGC	7 cut(s)	176, 403, 631, 706, 1396, 1507, 1629
GsaI	CCCAGC	1 cut(s)	301
GsuI	CTGGAG	2 cut(s)	1708, 2003
HaeII	RGCGCY	2 cut(s)	633, 1398
HaeIII	GGCC	9 cut(s)	267, 636, 871, 1358, 1440, 1855, 1879, 2116, 2200
HgaI	GACGC	2 cut(s)	1814, 2044
HhaI	GCGC	7 cut(s)	177, 404, 632, 707, 1397, 1508, 1630
Hin1I	GRCGYC	3 cut(s)	630, 1395, 2036
Hin1II	CATG	5 cut(s)	95, 547, 1285, 1319, 1649
Hin6I	GCGC	7 cut(s)	175, 402, 630, 705, 1395, 1506, 1628
HinP1I	GCGC	7 cut(s)	175, 402, 630, 705, 1395, 1506, 1628
HincII	GTYRAC	2 cut(s)	445, 1941
HindII	GTYRAC	2 cut(s)	445, 1941
HindIII	AAGCTT	1 cut(s)	1402
HinfI	GANTC	5 cut(s)	1514, 1859, 1929, 1965, 2132
HphI	GGTGA	4 cut(s)	43, 965, 2033, 2101
Hpy166II	GTNNAC	5 cut(s)	445, 694, 1606, 1941, 2160
Hpy8I	GTNNAC	5 cut(s)	445, 694, 1606, 1941, 2160
HpyAV	CCTTC	8 cut(s)	25, 49, 106, 238, 337, 1492, 1879, 2180
HpyCH4III	ACNGT	7 cut(s)	142, 205, 361, 412, 730, 1625, 1900
HpyCH4IV	ACGT	7 cut(s)	340, 365, 450, 579, 645, 1223, 1614
HpyF3I	CTNAG	3 cut(s)	379, 972, 2296
HpySE526I	ACGT	7 cut(s)	340, 365, 450, 579, 645, 1223, 1614
Hsp92I	GRCGYC	3 cut(s)	630, 1395, 2036
Hsp92II	CATG	5 cut(s)	95, 547, 1285, 1319, 1649
HspAI	GCGC	7 cut(s)	175, 402, 630, 705, 1395, 1506, 1628
KasI	GGCGCC	2 cut(s)	629, 1394
Kpn2I	TCCGGA	2 cut(s)	1915, 2119
KroI	GCCGGC	3 cut(s)	566, 632, 2065
KroNI	GCCGGC	3 cut(s)	568, 634, 2067
Ksp22I	TGATCA	1 cut(s)	1636
KspI	CCGCGG	3 cut(s)	170, 196, 1113
Kzo9I	GATC	7 cut(s)	588, 1247, 1278, 1452, 1636, 1701, 1912
LguI	GCTCTTC	1 cut(s)	1943
LmnI	GCTCC	5 cut(s)	113, 901, 1160, 1296, 1984
Lsp1109I	GCAGC	5 cut(s)	184, 689, 1087, 1089, 2277
LweI	GCATC	5 cut(s)	186, 297, 1183, 1708, 2277
MaeI	CTAG	2 cut(s)	1188, 1197
MaeII	ACGT	7 cut(s)	340, 365, 450, 579, 645, 1223, 1614
MaeIII	GTNAC	2 cut(s)	361, 415
MalI	GATC	7 cut(s)	590, 1249, 1280, 1454, 1638, 1703, 1914
MbiI	CCGCTC	2 cut(s)	273, 1299
MboI	GATC	7 cut(s)	588, 1247, 1278, 1452, 1636, 1701, 1912
MboII	GAAGA	7 cut(s)	58, 528, 914, 1489, 1495, 1960, 2011
MflI	RGATCY	1 cut(s)	1247
MhlI	GDGCHC	5 cut(s)	219, 906, 1274, 1353, 1614
Mly113I	GGCGCC	2 cut(s)	630, 1395
MlyI	GAGTC	3 cut(s)	1868, 1923, 1959
MmeI	TCCRAC	4 cut(s)	453, 789, 1138, 1549
Mph1103I	ATGCAT	1 cut(s)	2172
MroI	TCCGGA	2 cut(s)	1915, 2119
MroNI	GCCGGC	3 cut(s)	566, 632, 2065
MroXI	GAANNNNTTC	1 cut(s)	921
MseI	TTAA	4 cut(s)	765, 827, 1682, 2013
MslI	CAYNNNNRTG	3 cut(s)	548, 1644, 2045
MspA1I	CMGCKG	4 cut(s)	169, 195, 702, 1112
MspR9I	CCNGG	8 cut(s)	532, 617, 654, 835, 927, 1268, 1442, 2262
Mva1269I	GAATGC	1 cut(s)	1508
MvaI	CCWGG	4 cut(s)	654, 835, 1268, 2262
MvnI	CGCG	7 cut(s)	169, 195, 1112, 1355, 1808, 1820, 1946
NaeI	GCCGGC	3 cut(s)	568, 634, 2067
NarI	GGCGCC	2 cut(s)	630, 1395
NciI	CCSGG	4 cut(s)	532, 617, 927, 1442
NdeII	GATC	7 cut(s)	588, 1247, 1278, 1452, 1636, 1701, 1912
NgoMIV	GCCGGC	3 cut(s)	566, 632, 2065
NheI	GCTAGC	1 cut(s)	1196
NlaIII	CATG	5 cut(s)	95, 547, 1285, 1319, 1649
NlaIV	GGNNCC	7 cut(s)	103, 109, 139, 424, 631, 1396, 1854
NmeAIII	GCCGAG	1 cut(s)	1947
NmuCI	GTSAC	1 cut(s)	415
NotI	GCGGCCGC	1 cut(s)	1356
NsbI	TGCGCA	1 cut(s)	706
NsiI	ATGCAT	1 cut(s)	2172
NspI	RCATGY	1 cut(s)	1319
PaeI	GCATGC	1 cut(s)	1319
PaeR7I	CTCGAG	1 cut(s)	1273
PagI	TCATGA	1 cut(s)	1281
PciSI	GCTCTTC	1 cut(s)	1943
PcsI	WCGNNNNNNNCGW	3 cut(s)	453, 912, 1523
PctI	GAATGC	1 cut(s)	1508
PdiI	GCCGGC	3 cut(s)	568, 634, 2067
PdmI	GAANNNNTTC	1 cut(s)	921
PfeI	GAWTC	2 cut(s)	1514, 2132
PleI	GAGTC	3 cut(s)	1867, 1923, 1959
PluTI	GGCGCC	2 cut(s)	633, 1398
PmaCI	CACGTG	2 cut(s)	580, 1615
PmlI	CACGTG	2 cut(s)	580, 1615
PpsI	GAGTC	3 cut(s)	1867, 1923, 1959
Ppu21I	YACGTR	2 cut(s)	580, 1615
Psp124BI	GAGCTC	2 cut(s)	906, 1353
Psp6I	CCWGG	4 cut(s)	652, 833, 1266, 2260
PspCI	CACGTG	2 cut(s)	580, 1615
PspFI	CCCAGC	1 cut(s)	297
PspGI	CCWGG	4 cut(s)	652, 833, 1266, 2260
PspN4I	GGNNCC	7 cut(s)	103, 109, 139, 424, 631, 1396, 1854
PspPI	GGNCC	6 cut(s)	252, 475, 870, 1815, 1853, 2114
PspXI	VCTCGAGB	1 cut(s)	1273
PsuI	RGATCY	1 cut(s)	1247
PvuII	CAGCTG	1 cut(s)	702
RsaI	GTAC	3 cut(s)	862, 1607, 1922
RsaNI	GTAC	3 cut(s)	861, 1606, 1921
RseI	CAYNNNNRTG	3 cut(s)	548, 1644, 2045
SacI	GAGCTC	2 cut(s)	906, 1353
SacII	CCGCGG	3 cut(s)	170, 196, 1113
SalI	GTCGAC	1 cut(s)	443
SapI	GCTCTTC	1 cut(s)	1943
SaqAI	TTAA	4 cut(s)	765, 827, 1682, 2013
Sau3AI	GATC	7 cut(s)	588, 1247, 1278, 1452, 1636, 1701, 1912
Sau96I	GGNCC	6 cut(s)	252, 475, 870, 1815, 1853, 2114
ScaI	AGTACT	1 cut(s)	1922
SchI	GAGTC	3 cut(s)	1868, 1923, 1959
ScrFI	CCNGG	8 cut(s)	532, 617, 654, 835, 927, 1268, 1442, 2262
SduI	GDGCHC	5 cut(s)	219, 906, 1274, 1353, 1614
SfaNI	GCATC	5 cut(s)	186, 297, 1183, 1708, 2277
SfoI	GGCGCC	2 cut(s)	631, 1396
Sfr274I	CTCGAG	1 cut(s)	1273
Sfr303I	CCGCGG	3 cut(s)	170, 196, 1113
SgrBI	CCGCGG	3 cut(s)	170, 196, 1113
SinI	GGWCC	3 cut(s)	252, 475, 1815
SlaI	CTCGAG	1 cut(s)	1273
SmiMI	CAYNNNNRTG	3 cut(s)	548, 1644, 2045
SmlI	CTYRAG	2 cut(s)	1273, 2272
SmoI	CTYRAG	2 cut(s)	1273, 2272
SphI	GCATGC	1 cut(s)	1319
SspDI	GGCGCC	2 cut(s)	629, 1394
SspI	AATATT	1 cut(s)	2010
SspMI	CTAG	2 cut(s)	1188, 1197
SstI	GAGCTC	2 cut(s)	906, 1353
StyD4I	CCNGG	8 cut(s)	530, 615, 652, 833, 925, 1266, 1440, 2260
TaaI	ACNGT	7 cut(s)	142, 205, 361, 412, 730, 1625, 1900
TaiI	ACGT	7 cut(s)	343, 368, 453, 582, 648, 1226, 1617
TatI	WGTACW	1 cut(s)	1920
TfiI	GAWTC	2 cut(s)	1514, 2132
Tru1I	TTAA	4 cut(s)	765, 827, 1682, 2013
Tru9I	TTAA	4 cut(s)	765, 827, 1682, 2013
TscAI	CASTG	1 cut(s)	2067
TseFI	GTSAC	1 cut(s)	415
TseI	GCWGC	5 cut(s)	172, 702, 1075, 1102, 2265
Tsp45I	GTSAC	1 cut(s)	415
TspDTI	ATGAA	9 cut(s)	31, 483, 1023, 1496, 1532, 1760, 2090, 2145, 2268
TspGWI	ACGGA	2 cut(s)	265, 1941
TspRI	CASTG	1 cut(s)	2067
VpaK11BI	GGWCC	3 cut(s)	252, 475, 1815
XapI	RAATTY	4 cut(s)	838, 918, 963, 1684
XceI	RCATGY	1 cut(s)	1319
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2047
XhoI	CTCGAG	1 cut(s)	1273
XmiI	GTMKAC	2 cut(s)	444, 2159
XmnI	GAANNNNTTC	1 cut(s)	921
XspI	CTAG	2 cut(s)	1188, 1197
ZrmI	AGTACT	1 cut(s)	1922
Zsp2I	ATGCAT	1 cut(s)	2172

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.