Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0003366

Physical Location & Seq

Genomic Coordinates Reverse (-)

8337451 .. 8340828

Gene Structure 3378 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00287300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2373 bp

ATGACGAAACTACCCTTCTCCGTTCTCATTACCATCCTCACCTTCATCTTCACCATTTTGTTCACCCTCCCATTTTCCCATGCACTCGGCTCCGGCGCCACTCTCGCCGTCAGCTACGCCACCGGCGCCGTCTGCGGCTTGGTCTCCGGCCAACCCACTCAGCGCATAGTCTGCTACCGGCGAGGCCAAACCATCTCCGTCGAGCCCAACATCTCCTTCTCGTCAATCTCCGGCGGCCGGACCTCCCTGTGCGGCCTCCGGTCCGGCGGCTACGCCCTGCTCTGTTACGACAATCTACTCTTCCAAGACTCGGCCTTTGATTCGAAAAGAATCTACTTTAACGAAAGTGGTGAATTGGAAAACATAGCCGTCGGAGAAAACCATATTTGCGCCACCGTGGTGGGTTCGGGCGCCGTGAAATGTTGGCGAGAAGAGGACGGAACGTTTCAACTGCCATCTGGGTCGGAGCAATTCGGGTCCATCTCATCTGGGTCGGGCTTCTCCTGTGGAATTTCGAAGAACAGCTCGAGGGTCCGGTGCTGGGGGACTAGAACTTCCATGGCGGCCGAGATTGAAAATGGGTTTGGAGAAACGGTCATGGCGAGTATCGAAGCAGGTGCTTATCACGTGTGTGGAGTCAATGTTACTGGGTCAATAATCTGCAGAGGCAGCAACGACTCTGGTCAATTGAATGCACCGCCGGGAACGTTTTCCGGTTTAGCTCTCGGAGCTGAACACAGCTGTGCGATTAGAGATTTAAACCAGTCTGTGATTTGTTGGGGCGGTGGAGGCCAGTTTGGGCCTAATGTGACTTCAGCGACGTCGTTTGAGGTGATCGTTTCCGGGTCTAATTTCACTTGTGGATTAACCACAAGCAACTTTTCGATTATATGTTGGGGTCCTGGTTGGCCTAGTTCAGAGGTTGAAGGTGAGCTTGGTCTGGGGGAGATTCTTCCGGGTCGTTGTGTACACTCCTCTTGTAGTGAACGTGGTATCTATCCTCAATCTCAAAAGCTCTGTTATGGCTCTGGGAACATTTGCAATCCTTGGAAGCCGATTTTGGTTCCTAAATTACCACCCTCACCGTCGATTTCACCGCCGCCGGAATCTCCGTCCCGGGCTTTGAAAACGGGGTTGTTAGTGTTCTGCATTGTGGGTTCTATAGGAGGGTTTTCGGGGATTTGTAGTGTCATTTATTGTTTGTGGGTGGGTGGTTGTTTTGGGCAAAAGAAAGTTCATAATTCAGTTCAGCCTACAATCACCAGAGCTGGCAGTTCCAACCACGGGAACACATCAAATAACAGCCCTCCTTCGAGATCAGCCACCATAAGGCGGCAGAGCTCGAGGATCATGAGGCGGCAGAGGAGTGGGCCTTCGTCCAAGCACACAGATAGGGCTGAGGAGTTCTCACTGGCTGAGCTTGCAGCAGCTACAAATGGTTTTGCATTGGAGAACAAGATTGGTGCAGGGAGCTTTGGGGTGGTGTACAGAGGGAAATTGGATGACGGGCGCGAAGTTGCTATCAAGAGAGGAGAAACAGCAACCAAGGTGAAGAAGTACCAAGAGAAGGATACTGCTTTCGATTCAGAGTTGTCATTCTTGTCGAGGCTTCATCACAAGCATTTGGTTAGTCTTGTTGGGTATTGTGAGGAGAAGGAGGAGAGGCTGTTGGTTTATGAGTACATGAAGAATGGTGCGCTCCATGATCATTTACATGACAAGAACAATGTCGAGAAGAGTAGTAGTTTGATTAATTCTTGGAAAATGAGGATCAAGGTGGCTTTAGATGCTGCCAGAGGCATTGAGTATCTACACAACTATGCAGTCCCTCCTATAATTCACAGAGATATAAAGTCCTCTAACATTCTGATAGATGGAAATTGGACTGGAAGAGTATCTGATTTCGGATTGTCACTATTGGGTCCGGAAAATGACAGCGACTATAGGCCAATGAAGGCAGCTGGAACCGTCGGCTACATTGATCCAGAGTACTATGGTTTAAATTTATTAACAGCAAAGAGTGATGTGTATGGGCTTGGTGTGGTGTTGTTGGAGCTTCTGACAGGAAGAAGAGCAATCTTCAGGAACAGTGAAGACGGAGGGACACCTACCAGTGTTGTGGACTTCACTGTGCCGCTCATCATGGCCGGAGAGTTAGATAAGGTTTTGGACCCAAGGGTTGGACCTCCGGAAATCAGTGAAGCAGAGGCGGTTGAGTTGGTGGCGTATACTGCAATGCATTGTGTGAATTTGGAAGGTAAAGATAGACCAACCATGACTGACATTGTGGCCAATTTGGAGCGAGCATTGAGTCTCTGTGAGGAAAGCCCCGGTGGCAGCATGTCTAGCGGCTCAATCATCTCTGCTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr RCC1_2

Protein Analysis

790

Amino Acids

84.52

Weight (kDa)

6.31

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	482 - 763	2.1e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	483 - 765	3e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55950
fragaria_vesca	FvH4_3g38710 FvH4_7g24490
malus_domestica	MD00G1166500.v1.1 MD01G1153100.v1.1 MD03G1068800.v1.1 MD07G1222100.v1.1 MD11G1073300.v1.1
prunus_persica	Prupe.2G250300_v2.0.a1 Prupe.6G056600_v2.0.a1
pyrus_communis	pycom01g17070 pycom03g05510
rosa_chinensis	RchiOBHm_Chr1g0370251 RchiOBHm_Chr5g0065501 RchiOBHm_Chr5g0069861
rosa_laevigata	RLG00000027065 RLG00000036103
rosa_multiflora	Rmu_co8321277.1_g000001 Rmu_sc0000738.1_g000002 Rmu_sc0026893.1_g000001 Rmu_sc0037931.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011190 Rroxscaffold_4G00286710 Rroxscaffold_4G00287300
rosa_rugosa	Rorug01G0355200 Rorug05G0400300
rosa_samantha	Rh1AG363800 Rh1BG326900 Rh1CG340200 Rh1DG357300 Rh5AG457400 Rh5BG476200 Rh5CG500000 Rh5DG488000
rosa_wichuraiana	Rw1G032010 Rw5G042700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	605
AatII	GACGTC	1 cut(s)	824
Acc36I	ACCTGC	1 cut(s)	605
AccB1I	GGYRCC	3 cut(s)	95, 125, 410
AccB7I	CCANNNNNTGG	1 cut(s)	2180
AccBSI	CCGCTC	1 cut(s)	2137
AccI	GTMKAC	1 cut(s)	2228
AccII	CGCG	1 cut(s)	1512
AccIII	TCCGGA	2 cut(s)	1924, 2188
AclI	AACGTT	2 cut(s)	443, 707
AclWI	GGATC	3 cut(s)	1355, 1778, 1976
AcoI	YGGCCR	5 cut(s)	148, 235, 564, 2145, 2289
AcsI	RAATTY	3 cut(s)	510, 2002, 2248
AcuI	CTGAAG	2 cut(s)	798, 2065
AcvI	CACGTG	1 cut(s)	628
AcyI	GRCGYC	4 cut(s)	96, 126, 411, 821
AfaI	GTAC	5 cut(s)	969, 1487, 1559, 1682, 1991
AfiI	CCNNNNNNNGG	8 cut(s)	237, 310, 541, 1284, 1329, 1332, 1567, 2180
AflIII	ACRYGT	1 cut(s)	627
AgsI	TTSAA	5 cut(s)	449, 575, 691, 926, 1126
AjnI	CCWGG	1 cut(s)	901
AjuI	GAANNNNNNNTTGG	8 cut(s)	200, 232, 567, 599, 918, 950, 1043, 1075
AleI	CACNNNNGTG	3 cut(s)	398, 630, 741
Alw21I	GWGCWC	1 cut(s)	1343
Alw26I	GTCTC	2 cut(s)	148, 2318
AlwI	GGATC	3 cut(s)	1355, 1778, 1976
Ama87I	CYCGRG	3 cut(s)	526, 1116, 1342
Aor13HI	TCCGGA	2 cut(s)	1924, 2188
ApeKI	GCWGC	6 cut(s)	669, 1424, 1427, 1790, 1958, 2337
ApoI	RAATTY	3 cut(s)	510, 2002, 2248
ArsI	GACNNNNNNTTYG	2 cut(s)	299, 331
AseI	ATTAAT	1 cut(s)	1752
Asp700I	GAANNNNTTC	1 cut(s)	709
AspLEI	GCGC	7 cut(s)	98, 128, 165, 392, 413, 1512, 1699
AsuC2I	CCSGG	6 cut(s)	702, 844, 957, 1117, 1118, 2331
AsuII	TTCGAA	2 cut(s)	323, 515
AvaI	CYCGRG	3 cut(s)	526, 1116, 1342
AvaII	GGWCC	8 cut(s)	240, 261, 477, 532, 899, 1922, 2170, 2183
BalI	TGGCCA	1 cut(s)	2291
BanI	GGYRCC	3 cut(s)	95, 125, 410
BanII	GRGCYC	2 cut(s)	207, 1343
BbrPI	CACGTG	1 cut(s)	628
BbsI	GAAGAC	1 cut(s)	2100
Bbv12I	GWGCWC	1 cut(s)	1343
BbvCI	CCTCAGC	1 cut(s)	1398
BbvI	GCAGC	6 cut(s)	681, 1436, 1439, 1777, 1970, 2349
BccI	CCATC	5 cut(s)	41, 200, 463, 488, 1868
BceAI	ACGGC	4 cut(s)	92, 113, 353, 398
BcgI	CGANNNNNNTGC	2 cut(s)	599, 633
BciT130I	CCWGG	1 cut(s)	903
BciVI	GTATCC	1 cut(s)	1564
BclI	TGATCA	1 cut(s)	1705
BcnI	CCSGG	6 cut(s)	702, 844, 957, 1117, 1118, 2331
BcoDI	GTCTC	2 cut(s)	148, 2318
BfaI	CTAG	3 cut(s)	549, 912, 2346
BfmI	CTRYAG	3 cut(s)	661, 1161, 1942
BfoI	RGCGCY	3 cut(s)	99, 129, 414
BfuAI	ACCTGC	1 cut(s)	605
BfuI	GTATCC	1 cut(s)	1564
BglI	GCCNNNNNGGC	1 cut(s)	2334
BlpI	GCTNAGC	1 cut(s)	1416
BmcAI	AGTACT	1 cut(s)	1991
Bme1390I	CCNGG	7 cut(s)	702, 844, 903, 957, 1117, 1118, 2331
Bme18I	GGWCC	8 cut(s)	240, 261, 477, 532, 899, 1922, 2170, 2183
BmeT110I	CYCGRG	3 cut(s)	526, 1116, 1342
BmrFI	CCNGG	7 cut(s)	702, 844, 903, 957, 1117, 1118, 2331
BmrI	ACTGGG	1 cut(s)	657
BmsI	GCATC	1 cut(s)	1777
BmuI	ACTGGG	1 cut(s)	657
BoxI	GACNNNNGTC	1 cut(s)	681
BpiI	GAAGAC	1 cut(s)	2100
BplI	GAGNNNNNCTC	2 cut(s)	1391, 1423
Bpu10I	CCTNAGC	1 cut(s)	1398
Bpu1102I	GCTNAGC	1 cut(s)	1416
Bpu14I	TTCGAA	2 cut(s)	323, 515
BpuMI	CCSGG	6 cut(s)	702, 844, 957, 1117, 1118, 2331
BsaAI	YACGTR	1 cut(s)	628
BsaHI	GRCGYC	4 cut(s)	96, 126, 411, 821
BsaI	GGTCTC	1 cut(s)	148
BsaJI	CCNNGG	8 cut(s)	396, 558, 1046, 1116, 1282, 1545, 2174, 2329
BsaWI	WCCGGW	5 cut(s)	258, 534, 713, 1924, 2188
BsaXI	ACNNNNNCTCC	6 cut(s)	579, 609, 627, 657, 1652, 1682
Bsc4I	CCNNNNNNNGG	8 cut(s)	237, 310, 541, 1284, 1329, 1332, 1567, 2180
Bse118I	RCCGGY	2 cut(s)	122, 177
Bse1I	ACTGG	6 cut(s)	652, 763, 793, 1416, 1891, 2112
Bse3DI	GCAATG	1 cut(s)	2241
BseAI	TCCGGA	2 cut(s)	1924, 2188
BseBI	CCWGG	1 cut(s)	903
BseDI	CCNNGG	8 cut(s)	396, 558, 1046, 1116, 1282, 1545, 2174, 2329
BseGI	GGATG	2 cut(s)	33, 1507
BseLI	CCNNNNNNNGG	8 cut(s)	237, 310, 541, 1284, 1329, 1332, 1567, 2180
BseMI	GCAATG	1 cut(s)	2241
BseMII	CTCAG	3 cut(s)	173, 1389, 1407
BseNI	ACTGG	6 cut(s)	652, 763, 793, 1416, 1891, 2112
BseRI	GAGGAG	6 cut(s)	964, 1378, 1415, 1545, 1664, 1673
BseX3I	CGGCCG	2 cut(s)	235, 564
BseXI	GCAGC	6 cut(s)	681, 1436, 1439, 1777, 1970, 2349
BseYI	CCCAGC	1 cut(s)	540
BsgI	GTGCAG	1 cut(s)	1485
Bsh1236I	CGCG	1 cut(s)	1512
Bsh1285I	CGRYCG	2 cut(s)	238, 567
BshNI	GGYRCC	3 cut(s)	95, 125, 410
BsiEI	CGRYCG	2 cut(s)	238, 567
BsiHKAI	GWGCWC	1 cut(s)	1343
BsiHKCI	CYCGRG	3 cut(s)	526, 1116, 1342
BslFI	GGGAC	4 cut(s)	559, 1099, 1811, 2116
BslI	CCNNNNNNNGG	8 cut(s)	237, 310, 541, 1284, 1329, 1332, 1567, 2180
BsmAI	GTCTC	2 cut(s)	148, 2318
BsmFI	GGGAC	4 cut(s)	559, 1099, 1811, 2116
BsmI	GAATGC	1 cut(s)	697
Bso31I	GGTCTC	1 cut(s)	148
BsoBI	CYCGRG	3 cut(s)	526, 1116, 1342
Bsp119I	TTCGAA	2 cut(s)	323, 515
Bsp1286I	GDGCHC	2 cut(s)	207, 1343
Bsp13I	TCCGGA	2 cut(s)	1924, 2188
Bsp1407I	TGTACA	2 cut(s)	967, 1485
Bsp143I	GATC	6 cut(s)	834, 1316, 1347, 1705, 1770, 1981
Bsp1720I	GCTNAGC	1 cut(s)	1416
Bsp19I	CCATGG	1 cut(s)	558
BspCNI	CTCAG	3 cut(s)	172, 1390, 1408
BspEI	TCCGGA	2 cut(s)	1924, 2188
BspFNI	CGCG	1 cut(s)	1512
BspHI	TCATGA	1 cut(s)	1350
BspMAI	CTGCAG	1 cut(s)	665
BspMI	ACCTGC	1 cut(s)	605
BspPI	GGATC	3 cut(s)	1355, 1778, 1976
BspQI	GCTCTTC	1 cut(s)	2065
BspT104I	TTCGAA	2 cut(s)	323, 515
BspT107I	GGYRCC	3 cut(s)	95, 125, 410
BspTNI	GGTCTC	1 cut(s)	148
BsrBI	CCGCTC	1 cut(s)	2137
BsrDI	GCAATG	1 cut(s)	2241
BsrFI	RCCGGY	2 cut(s)	122, 177
BsrGI	TGTACA	2 cut(s)	967, 1485
BsrI	ACTGG	6 cut(s)	652, 763, 793, 1416, 1891, 2112
BssAI	RCCGGY	2 cut(s)	122, 177
BssECI	CCNNGG	8 cut(s)	396, 558, 1046, 1116, 1282, 1545, 2174, 2329
BssMI	GATC	6 cut(s)	834, 1316, 1347, 1705, 1770, 1981
BssNAI	GTATAC	1 cut(s)	2229
BssNI	GRCGYC	4 cut(s)	96, 126, 411, 821
BssT1I	CCWWGG	4 cut(s)	558, 1046, 1545, 2174
Bst1107I	GTATAC	1 cut(s)	2229
Bst2UI	CCWGG	1 cut(s)	903
Bst4CI	ACNGT	6 cut(s)	397, 595, 1086, 1969, 2090, 2131
Bst6I	CTCTTC	5 cut(s)	305, 426, 1730, 1885, 2065
BstACI	GRCGYC	4 cut(s)	96, 126, 411, 821
BstAPI	GCANNNNNTGC	1 cut(s)	171
BstAUI	TGTACA	2 cut(s)	967, 1485
BstBAI	YACGTR	1 cut(s)	628
BstBI	TTCGAA	2 cut(s)	323, 515
BstC8I	GCNNGC	3 cut(s)	1270, 1422, 2304
BstDEI	CTNAG	3 cut(s)	159, 1398, 1416
BstDSI	CCRYGG	3 cut(s)	396, 558, 1282
BstF5I	GGATG	2 cut(s)	33, 1507
BstFNI	CGCG	1 cut(s)	1512
BstH2I	RGCGCY	3 cut(s)	99, 129, 414
BstHHI	GCGC	7 cut(s)	98, 128, 165, 392, 413, 1512, 1699
BstKTI	GATC	6 cut(s)	837, 1319, 1350, 1708, 1773, 1984
BstMAI	GTCTC	2 cut(s)	148, 2318
BstMBI	GATC	6 cut(s)	834, 1316, 1347, 1705, 1770, 1981
BstMCI	CGRYCG	2 cut(s)	238, 567
BstNI	CCWGG	1 cut(s)	903
BstNSI	RCATGY	1 cut(s)	2344
BstPAI	GACNNNNGTC	1 cut(s)	681
BstSCI	CCNGG	7 cut(s)	700, 842, 901, 955, 1115, 1116, 2329
BstSFI	CTRYAG	3 cut(s)	661, 1161, 1942
BstUI	CGCG	1 cut(s)	1512
BstV1I	GCAGC	6 cut(s)	681, 1436, 1439, 1777, 1970, 2349
BstV2I	GAAGAC	1 cut(s)	2100
BstXI	CCANNNNNNTGG	2 cut(s)	400, 2119
BstZ17I	GTATAC	1 cut(s)	2229
BstZI	CGGCCG	2 cut(s)	235, 564
BsuI	GTATCC	1 cut(s)	1564
BtgI	CCRYGG	3 cut(s)	396, 558, 1282
BtsCI	GGATG	2 cut(s)	33, 1507
BtsIMutI	CAGTG	5 cut(s)	1409, 2095, 2119, 2127, 2203
BveI	ACCTGC	1 cut(s)	605
Cac8I	GCNNGC	3 cut(s)	1270, 1422, 2304
CciI	TCATGA	1 cut(s)	1350
CfoI	GCGC	7 cut(s)	98, 128, 165, 392, 413, 1512, 1699
Cfr10I	RCCGGY	2 cut(s)	122, 177
Cfr9I	CCCGGG	1 cut(s)	1116
CpoI	CGGWCCG	1 cut(s)	261
Csp6I	GTAC	5 cut(s)	968, 1486, 1558, 1681, 1990
CspI	CGGWCCG	1 cut(s)	261
CviQI	GTAC	5 cut(s)	968, 1486, 1558, 1681, 1990
DdeI	CTNAG	3 cut(s)	159, 1398, 1416
DinI	GGCGCC	3 cut(s)	97, 127, 412
DpnI	GATC	6 cut(s)	836, 1318, 1349, 1707, 1772, 1983
DpnII	GATC	6 cut(s)	834, 1316, 1347, 1705, 1770, 1981
DraI	TTTAAA	2 cut(s)	759, 2001
EaeI	YGGCCR	5 cut(s)	148, 235, 564, 2145, 2289
EagI	CGGCCG	2 cut(s)	235, 564
Eam1104I	CTCTTC	5 cut(s)	305, 426, 1730, 1885, 2065
EarI	CTCTTC	5 cut(s)	305, 426, 1730, 1885, 2065
Ecl136II	GAGCTC	1 cut(s)	1341
EclXI	CGGCCG	2 cut(s)	235, 564
Eco130I	CCWWGG	4 cut(s)	558, 1046, 1545, 2174
Eco24I	GRGCYC	2 cut(s)	207, 1343
Eco31I	GGTCTC	1 cut(s)	148
Eco47I	GGWCC	8 cut(s)	240, 261, 477, 532, 899, 1922, 2170, 2183
Eco52I	CGGCCG	2 cut(s)	235, 564
Eco53kI	GAGCTC	1 cut(s)	1341
Eco57I	CTGAAG	2 cut(s)	798, 2065
Eco72I	CACGTG	1 cut(s)	628
Eco88I	CYCGRG	3 cut(s)	526, 1116, 1342
EcoICRI	GAGCTC	1 cut(s)	1341
EcoO109I	RGGNCCY	1 cut(s)	899
EcoRII	CCWGG	1 cut(s)	901
EcoT14I	CCWWGG	4 cut(s)	558, 1046, 1545, 2174
EcoT22I	ATGCAT	1 cut(s)	2241
EcoT38I	GRGCYC	2 cut(s)	207, 1343
EgeI	GGCGCC	3 cut(s)	97, 127, 412
EheI	GGCGCC	3 cut(s)	97, 127, 412
ErhI	CCWWGG	4 cut(s)	558, 1046, 1545, 2174
FalI	AAGNNNNNCTT	2 cut(s)	918, 950
FaqI	GGGAC	4 cut(s)	559, 1099, 1811, 2116
FbaI	TGATCA	1 cut(s)	1705
FblI	GTMKAC	1 cut(s)	2228
FokI	GGATG	2 cut(s)	20, 1514
FriOI	GRGCYC	2 cut(s)	207, 1343
FspBI	CTAG	3 cut(s)	549, 912, 2346
GlaI	GCGC	7 cut(s)	97, 127, 164, 391, 412, 1511, 1698
GsaI	CCCAGC	1 cut(s)	544
HaeII	RGCGCY	3 cut(s)	99, 129, 414
HhaI	GCGC	7 cut(s)	98, 128, 165, 392, 413, 1512, 1699
Hin1I	GRCGYC	4 cut(s)	96, 126, 411, 821
Hin6I	GCGC	7 cut(s)	96, 126, 163, 390, 411, 1510, 1697
HinP1I	GCGC	7 cut(s)	96, 126, 163, 390, 411, 1510, 1697
HinfI	GANTC	9 cut(s)	308, 320, 330, 636, 677, 949, 1106, 1583, 2311
Hpy166II	GTNNAC	7 cut(s)	63, 968, 970, 986, 1486, 2122, 2229
Hpy188I	TCNGA	9 cut(s)	374, 466, 728, 919, 1588, 1869, 1900, 1907, 2061
Hpy188III	TCNNGA	8 cut(s)	1314, 1351, 1525, 1732, 1925, 1985, 2083, 2189
Hpy8I	GTNNAC	7 cut(s)	63, 968, 970, 986, 1486, 2122, 2229
Hpy99I	CGWCG	6 cut(s)	203, 374, 823, 826, 1090, 1973
HpyCH4III	ACNGT	6 cut(s)	397, 595, 1086, 1969, 2090, 2131
HpyCH4IV	ACGT	5 cut(s)	443, 627, 707, 821, 988
HpyF3I	CTNAG	3 cut(s)	159, 1398, 1416
HpySE526I	ACGT	5 cut(s)	443, 627, 707, 821, 988
Hsp92I	GRCGYC	4 cut(s)	96, 126, 411, 821
HspAI	GCGC	7 cut(s)	96, 126, 163, 390, 411, 1510, 1697
KasI	GGCGCC	3 cut(s)	95, 125, 410
Kpn2I	TCCGGA	2 cut(s)	1924, 2188
Ksp22I	TGATCA	1 cut(s)	1705
Kzo9I	GATC	6 cut(s)	834, 1316, 1347, 1705, 1770, 1981
LguI	GCTCTTC	1 cut(s)	2065
LmnI	GCTCC	7 cut(s)	95, 466, 728, 1470, 1704, 2053, 2299
Lsp1109I	GCAGC	6 cut(s)	681, 1436, 1439, 1777, 1970, 2349
LweI	GCATC	1 cut(s)	1777
MaeI	CTAG	3 cut(s)	549, 912, 2346
MaeII	ACGT	5 cut(s)	443, 627, 707, 821, 988
MaeIII	GTNAC	4 cut(s)	284, 643, 808, 1911
MalI	GATC	6 cut(s)	836, 1318, 1349, 1707, 1772, 1983
MbiI	CCGCTC	1 cut(s)	2137
MboI	GATC	6 cut(s)	834, 1316, 1347, 1705, 1770, 1981
MfeI	CAATTG	1 cut(s)	686
MhlI	GDGCHC	2 cut(s)	207, 1343
MlsI	TGGCCA	1 cut(s)	2291
MluNI	TGGCCA	1 cut(s)	2291
Mly113I	GGCGCC	3 cut(s)	96, 126, 411
MlyI	GAGTC	4 cut(s)	302, 645, 671, 2320
MmeI	TCCRAC	5 cut(s)	352, 444, 1302, 2031, 2161
Mox20I	TGGCCA	1 cut(s)	2291
Mph1103I	ATGCAT	1 cut(s)	2241
MroI	TCCGGA	2 cut(s)	1924, 2188
MroXI	GAANNNNTTC	1 cut(s)	709
MscI	TGGCCA	1 cut(s)	2291
MseI	TTAA	6 cut(s)	339, 758, 866, 1752, 2000, 2009
MslI	CAYNNNNRTG	5 cut(s)	398, 630, 741, 1713, 1818
Msp20I	TGGCCA	1 cut(s)	2291
MspA1I	CMGCKG	2 cut(s)	741, 1961
MspR9I	CCNGG	7 cut(s)	702, 844, 903, 957, 1117, 1118, 2331
MunI	CAATTG	1 cut(s)	686
Mva1269I	GAATGC	1 cut(s)	697
MvaI	CCWGG	1 cut(s)	903
MvnI	CGCG	1 cut(s)	1512
NarI	GGCGCC	3 cut(s)	96, 126, 411
NciI	CCSGG	6 cut(s)	702, 844, 957, 1117, 1118, 2331
NcoI	CCATGG	1 cut(s)	558
NdeII	GATC	6 cut(s)	834, 1316, 1347, 1705, 1770, 1981
NmeAIII	GCCGAG	3 cut(s)	66, 290, 592
NmuCI	GTSAC	2 cut(s)	808, 1911
NsiI	ATGCAT	1 cut(s)	2241
NspI	RCATGY	1 cut(s)	2344
NspV	TTCGAA	2 cut(s)	323, 515
OliI	CACNNNNGTG	3 cut(s)	398, 630, 741
PaeR7I	CTCGAG	2 cut(s)	526, 1342
PagI	TCATGA	1 cut(s)	1350
PaqCI	CACCTGC	1 cut(s)	605
PciSI	GCTCTTC	1 cut(s)	2065
PcsI	WCGNNNNNNNCGW	1 cut(s)	599
PctI	GAATGC	1 cut(s)	697
PdmI	GAANNNNTTC	1 cut(s)	709
PfeI	GAWTC	5 cut(s)	320, 330, 949, 1106, 1583
PflMI	CCANNNNNTGG	1 cut(s)	2180
PleI	GAGTC	4 cut(s)	302, 644, 671, 2319
PluTI	GGCGCC	3 cut(s)	99, 129, 414
PmaCI	CACGTG	1 cut(s)	628
PmlI	CACGTG	1 cut(s)	628
PpsI	GAGTC	4 cut(s)	302, 644, 671, 2319
Ppu21I	YACGTR	1 cut(s)	628
PpuMI	RGGWCCY	1 cut(s)	899
PshAI	GACNNNNGTC	1 cut(s)	681
PshBI	ATTAAT	1 cut(s)	1752
Psp124BI	GAGCTC	1 cut(s)	1343
Psp1406I	AACGTT	2 cut(s)	443, 707
Psp5II	RGGWCCY	1 cut(s)	899
Psp6I	CCWGG	1 cut(s)	901
PspCI	CACGTG	1 cut(s)	628
PspFI	CCCAGC	1 cut(s)	540
PspGI	CCWGG	1 cut(s)	901
PspPPI	RGGWCCY	1 cut(s)	899
PspXI	VCTCGAGB	2 cut(s)	526, 1342
PstI	CTGCAG	1 cut(s)	665
PvuII	CAGCTG	2 cut(s)	741, 1961
RsaI	GTAC	5 cut(s)	969, 1487, 1559, 1682, 1991
RsaNI	GTAC	5 cut(s)	968, 1486, 1558, 1681, 1990
RseI	CAYNNNNRTG	5 cut(s)	398, 630, 741, 1713, 1818
Rsr2I	CGGWCCG	1 cut(s)	261
RsrII	CGGWCCG	1 cut(s)	261
SacI	GAGCTC	1 cut(s)	1343
SapI	GCTCTTC	1 cut(s)	2065
SaqAI	TTAA	6 cut(s)	339, 758, 866, 1752, 2000, 2009
Sau3AI	GATC	6 cut(s)	834, 1316, 1347, 1705, 1770, 1981
ScaI	AGTACT	1 cut(s)	1991
SchI	GAGTC	4 cut(s)	302, 645, 671, 2320
ScrFI	CCNGG	7 cut(s)	702, 844, 903, 957, 1117, 1118, 2331
SduI	GDGCHC	2 cut(s)	207, 1343
SfaNI	GCATC	1 cut(s)	1777
SfcI	CTRYAG	3 cut(s)	661, 1161, 1942
SfoI	GGCGCC	3 cut(s)	97, 127, 412
Sfr274I	CTCGAG	2 cut(s)	526, 1342
SfuI	TTCGAA	2 cut(s)	323, 515
SgrAI	CRCCGGYG	1 cut(s)	122
SinI	GGWCC	8 cut(s)	240, 261, 477, 532, 899, 1922, 2170, 2183
SlaI	CTCGAG	2 cut(s)	526, 1342
SmaI	CCCGGG	1 cut(s)	1118
SmiMI	CAYNNNNRTG	5 cut(s)	398, 630, 741, 1713, 1818
SmlI	CTYRAG	2 cut(s)	526, 1342
SmoI	CTYRAG	2 cut(s)	526, 1342
SspDI	GGCGCC	3 cut(s)	95, 125, 410
SspMI	CTAG	3 cut(s)	549, 912, 2346
SstI	GAGCTC	1 cut(s)	1343
StyD4I	CCNGG	7 cut(s)	700, 842, 901, 955, 1115, 1116, 2329
StyI	CCWWGG	4 cut(s)	558, 1046, 1545, 2174
TaaI	ACNGT	6 cut(s)	397, 595, 1086, 1969, 2090, 2131
TaiI	ACGT	5 cut(s)	446, 630, 710, 824, 991
TatI	WGTACW	4 cut(s)	967, 1485, 1680, 1989
TfiI	GAWTC	5 cut(s)	320, 330, 949, 1106, 1583
Tru1I	TTAA	6 cut(s)	339, 758, 866, 1752, 2000, 2009
Tru9I	TTAA	6 cut(s)	339, 758, 866, 1752, 2000, 2009
TscAI	CASTG	5 cut(s)	1416, 2095, 2119, 2134, 2203
TseFI	GTSAC	2 cut(s)	808, 1911
TseI	GCWGC	6 cut(s)	669, 1424, 1427, 1790, 1958, 2337
Tsp45I	GTSAC	2 cut(s)	808, 1911
TspDTI	ATGAA	5 cut(s)	34, 1226, 1601, 1700, 1967
TspGWI	ACGGA	5 cut(s)	10, 187, 453, 1101, 2112
TspMI	CCCGGG	1 cut(s)	1116
TspRI	CASTG	5 cut(s)	1416, 2095, 2119, 2134, 2203
Van91I	CCANNNNNTGG	1 cut(s)	2180
VpaK11BI	GGWCC	8 cut(s)	240, 261, 477, 532, 899, 1922, 2170, 2183
VspI	ATTAAT	1 cut(s)	1752
XapI	RAATTY	3 cut(s)	510, 2002, 2248
XceI	RCATGY	1 cut(s)	2344
XhoI	CTCGAG	2 cut(s)	526, 1342
XmaI	CCCGGG	1 cut(s)	1116
XmiI	GTMKAC	1 cut(s)	2228
XmnI	GAANNNNTTC	1 cut(s)	709
XspI	CTAG	3 cut(s)	549, 912, 2346
ZraI	GACGTC	1 cut(s)	822
ZrmI	AGTACT	1 cut(s)	1991
Zsp2I	ATGCAT	1 cut(s)	2241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.