Rorug01G0355200

Serine threonine-protein kinase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003366
Physical Location & Seq
Reverse (-)
47177916 .. 47178440
525 bp
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UTR
Exon/CDS
Intron
Rorug01G0355200.1

Sequence Viewer

Length: 525 bp
ATGGAACTCTTCATGGAAATGTGTCTACTCCCGCTTTCATTTGCTTTCTCCTGCCTCTGTAACTTAGTTCTCCAAAGGAGAGACCGGTGCTGTTATCTGCTGGCCTATGAGTGCTACAAGGCCACAGAGGACATGATGCTCAACAGTGATTCTTGTGCCAAACTCGTCTTGCGGAACAAGAATCTGGGATTGGAAGAGTTCAGGTTTCTCTGGAAAACCATTGTCAATTCAGGCTTAGGTGAAGAAACTTCTTGCCCAAGAAACATCGTCCAAGGCCGAGAAGAATGTCCAACTCGAGAAGACTCGCTCACAGAAATGGATGAAATCATCTTTGCCACAGTTGACAAGCTTTTTGCCAGGAACAGTTCAATCCCTCCATCACAAATTGACACATTCTCGTCGTCAATGTCTTCATGTTCTCCCCTGCACCTTCTCTACATCCAGAATACTAAATCGTTACAAGATGAGGGAGGACATCAAGACCTTCAACCTAGCTCTCTGGAATGGGCTGCAGTGCAAGTCTAG

Protein Analysis

174

Amino Acids

19.79

Weight (kDa)

4.7

Isoelectric Point (pI)

69.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 24 - 141 2.2e-26 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 25
AciI CCGC 2 cut(s) 32, 172
AgeI ACCGGT 1 cut(s) 84
AgsI TTSAA 2 cut(s) 369, 488
AjnI CCWGG 1 cut(s) 356
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 2 cut(s) 349, 495
AluI AGCT 2 cut(s) 349, 495
Alw26I GTCTC 1 cut(s) 75
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 3 cut(s) 102, 120, 274
ApeKI GCWGC 1 cut(s) 509
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 251
AvaI CYCGRG 1 cut(s) 294
BbsI GAAGAC 2 cut(s) 306, 402
BbvI GCAGC 1 cut(s) 496
BccI CCATC 1 cut(s) 385
BciT130I CCWGG 1 cut(s) 358
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 2 cut(s) 492, 523
BfmI CTRYAG 1 cut(s) 510
BisI GCNGC 1 cut(s) 510
BlsI GCNGC 1 cut(s) 511
Bme1390I CCNGG 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 294
BmrFI CCNGG 1 cut(s) 358
BmsI GCATC 1 cut(s) 126
BpiI GAAGAC 2 cut(s) 306, 402
Bpu10I CCTNAGC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 271
BsaWI WCCGGW 1 cut(s) 84
Bse118I RCCGGY 1 cut(s) 84
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 2 cut(s) 325, 438
BseXI GCAGC 1 cut(s) 496
BsgI GTGCAG 1 cut(s) 410
BshFI GGCC 3 cut(s) 104, 122, 276
BshTI ACCGGT 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 75
BsnI GGCC 3 cut(s) 104, 122, 276
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 294
BspACI CCGC 2 cut(s) 32, 172
BspANI GGCC 3 cut(s) 104, 122, 276
BspMAI CTGCAG 1 cut(s) 514
BspTNI GGTCTC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 3 cut(s) 146, 340, 365
Bst6I CTCTTC 2 cut(s) 14, 189
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 2 cut(s) 64, 235
BstF5I GGATG 2 cut(s) 325, 438
BstMAI GTCTC 1 cut(s) 75
BstNI CCWGG 1 cut(s) 358
BstSCI CCNGG 1 cut(s) 356
BstSFI CTRYAG 1 cut(s) 510
BstV1I GCAGC 1 cut(s) 496
BstV2I GAAGAC 2 cut(s) 306, 402
BsuRI GGCC 3 cut(s) 104, 122, 276
BtsCI GGATG 2 cut(s) 325, 438
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 2 cut(s) 151, 519
Cac8I GCNNGC 1 cut(s) 102
Cfr10I RCCGGY 1 cut(s) 84
CspAI ACCGGT 1 cut(s) 84
CviAII CATG 3 cut(s) 13, 133, 414
CviJI RGCY 7 cut(s) 104, 122, 234, 276, 349, 495, 509
CviKI_1 RGCY 7 cut(s) 104, 122, 234, 276, 349, 495, 509
DdeI CTNAG 2 cut(s) 64, 235
Eam1104I CTCTTC 2 cut(s) 14, 189
EarI CTCTTC 2 cut(s) 14, 189
Eco130I CCWWGG 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 3 cut(s) 16, 136, 417
FaiI YATR 4 cut(s) 14, 108, 134, 415
FatI CATG 3 cut(s) 12, 132, 413
FauI CCCGC 1 cut(s) 39
FblI GTMKAC 1 cut(s) 25
Fnu4HI GCNGC 1 cut(s) 510
FokI GGATG 2 cut(s) 332, 425
Fsp4HI GCNGC 1 cut(s) 510
FspBI CTAG 2 cut(s) 492, 523
GluI GCNGC 1 cut(s) 510
HaeIII GGCC 3 cut(s) 104, 122, 276
HapII CCGG 1 cut(s) 85
Hin1II CATG 3 cut(s) 16, 136, 417
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 3 cut(s) 149, 181, 302
HpaII CCGG 1 cut(s) 85
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 2 cut(s) 26, 343
Hpy188III TCNNGA 5 cut(s) 211, 296, 442, 479, 500
Hpy8I GTNNAC 2 cut(s) 26, 343
Hpy99I CGWCG 1 cut(s) 403
HpyAV CCTTC 2 cut(s) 440, 494
HpyCH4III ACNGT 3 cut(s) 146, 340, 365
HpyCH4V TGCA 3 cut(s) 427, 512, 517
HpyF3I CTNAG 2 cut(s) 64, 235
Hsp92II CATG 3 cut(s) 16, 136, 417
Lsp1109I GCAGC 1 cut(s) 496
LweI GCATC 1 cut(s) 126
MaeI CTAG 2 cut(s) 492, 523
MaeIII GTNAC 2 cut(s) 59, 456
MboII GAAGA 5 cut(s) 206, 254, 293, 311, 402
MluCI AATT 2 cut(s) 226, 384
MlyI GAGTC 1 cut(s) 296
MmeI TCCRAC 1 cut(s) 314
MnlI CCTC 5 cut(s) 65, 121, 384, 460, 464
MslI CAYNNNNRTG 2 cut(s) 17, 314
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 358
MvaI CCWGG 1 cut(s) 358
NlaIII CATG 3 cut(s) 16, 136, 417
NmeAIII GCCGAG 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 294
PfeI GAWTC 2 cut(s) 149, 181
PinAI ACCGGT 1 cut(s) 84
PkrI GCNGC 1 cut(s) 511
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 356
PstI CTGCAG 1 cut(s) 514
RseI CAYNNNNRTG 2 cut(s) 17, 314
SatI GCNGC 1 cut(s) 510
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 1 cut(s) 358
SetI ASST 7 cut(s) 206, 241, 351, 432, 486, 493, 497
SfaNI GCATC 1 cut(s) 126
SfcI CTRYAG 1 cut(s) 510
Sfr274I CTCGAG 1 cut(s) 294
SlaI CTCGAG 1 cut(s) 294
SmiMI CAYNNNNRTG 2 cut(s) 17, 314
SmlI CTYRAG 1 cut(s) 294
SmoI CTYRAG 1 cut(s) 294
Sse9I AATT 2 cut(s) 226, 384
SsiI CCGC 2 cut(s) 32, 172
SspMI CTAG 2 cut(s) 492, 523
StyD4I CCNGG 1 cut(s) 356
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 3 cut(s) 146, 340, 365
TaqI TCGA 1 cut(s) 295
TasI AATT 2 cut(s) 226, 384
TfiI GAWTC 2 cut(s) 149, 181
TscAI CASTG 2 cut(s) 151, 519
TseI GCWGC 1 cut(s) 509
TspDTI ATGAA 3 cut(s) 27, 336, 402
TspRI CASTG 2 cut(s) 151, 519
XhoI CTCGAG 1 cut(s) 294
XmiI GTMKAC 1 cut(s) 25
XspI CTAG 2 cut(s) 492, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.