Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003366

Physical Location & Seq

Genomic Coordinates Forward (+)

6222597 .. 6225002

Gene Structure 2406 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1073300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2406 bp

ATGACACTTCCACGCGAACCCTTCTCTCTCACCGCCGCAATCTTGATCACAACCTTCATATCTTCTCTCATCACACCACATGATTGGTGGTTGGCCGACGCCCTTGGCTTAGCCTCCACCACAGCTATCACATATGGCACTCCGACGGTTTGTGGCATTGTGGCTGAGAGCCCCACACAGAGCATACAGTGCTTTCAAAACGGTCAGATTGCCATTTCAGTCCAACCCAATATTTCTTTTCAATCCATTTCTGGAGGGAAGAGCTTCTTCTGTGGCCTCAGGTCTGGAGGGTTTGCCCTTCTCTGCTGGGAGACCAGCTTGTCTAGCTCAAATAGGTTTCGGCCGAAACTAATATATCACAGTGGAAGTGTAGCTTTGACTGATCTGGCTGTGGGTGATGAGCAAGTTTGCGCTAGGGAAGTCAACTCTAGAATGGTCAGGTGCTGGAGAGGAAGAGATAATGCAAGTGGGGTTTTGTTTTCCTCACCTGGGGAAGCATTGTGGTTTCAAACAATCACATCCGGAAGCGGGTTTTCGTGTGGGATTTTGTTGAATGATGGGAGAGTTCTGTGTTGGGGCAAGAGTGATGTTGGAGCTGAGATTCAAACAGGGTTTGAGAAAGTGAACATGTCAAGCCTTGTTGCAGGCGGATCTCATGCTTGTGGCTTGAGTATGAATGGAACATTGGTCTGCAGAGGAAACAACGATTTCGGGCAATTGAATGTACCTTCAAGTACTTCGACTTTCCAGTTTTCAGGCCTTGCGTTGGGGGCAAATTTTACTTGTGCCATTCGGCGAAAAAATGGATTCGTTGTATGCTGGGGAGGAAGAAACATATTTGAATATGACAGTGTTGTAATTACAAATGTTTCATTTGAGTTAATTTCTGCAGGTTTGAATTTCGTGTGCGGCGTGACAACGAGTAATTTATCAGTGATTTGTTGGGGACCAGGGTGGTCTAGTTCGCATGATAATCTTCCACTGGGAATGATTATTCCTGGTCCATGTGTGCAGGGTCCATGTACTAGCTGTGTGTACCCGAATTCGGAGACTCTTTGTGGTGGTTCGGGGGAAATATGCAGATCATGCCGGGCTGAACTTCCATTAGCACTTCCATTGCCTCCAATAAATCAACCTACACAAGCTTCAAAGCCGGGTTTTTCGTTATTCAAAACAAAAAATAGGCTTCTGTTGGTTTTTGCAATAGTTGGATCAGTTGGGACATTTGCAGGACTCTGCACTATTGTCTTTTGCCTACGGACTGGACTCTGTGGTTCTTGGTTAAACTGCCATGACTCTGTACGAACCGAAAGTTTTCCTGATCCAAATGTGGGTTGTTCTGCTGCTAGAGTCGCCAATGTTCCTACTTCATTAGCATCAAGTTCCATGAAGGGCAGTTTAAGTGGTTCATCGTCGAAGCATGGTGACAAGATTGAGTCATTTCACCTGGTAGAGCTTTCCACTGCCACCAAATATTTCTCCACCGAAAACAAGATCGGTGCTGGGAGCTTTGGCATTGTCTATAAAGGCAAGCTTGCGGATGGTCGTGAAGTGGCCATCAAGAGGGGACATACAAGTACCAGGAACAAGAAATTTCAGGAGAAAGAGAGCGCGTTTGACTCCGAATTAGCATTGCTGTCCCGGCTTCACCACAAACACCTGGTGAAGCTAGTGGGATCCTGTGAAGCTAACGACGAGAGGCTTTTGGTTTACGAGTACATGAGCAACGGTTCACTTCATGATCACTTGCATAGCAAGGAAAATGTGGAGAAAAATAGCAGCAATGTGAATTCTTGGAAAATGAGGATAAAAATCGCGCTTGGTGCAGCCAGGGGAGTAGAGTATCTTCACAATTATGCAGTGCCAACAATAATTCACAGGGACATCAAGTCCTCAAACATTCTTTTGGATGCAAATTGGAATGCAAGGGTATCAGATTTTGGACTGTCATTATTGGGGCCGGGATCAAACCAAGAGATCATGTCGTCCAAAGCTGTTGGAACAATTGGGTACATTGATCCCGAGTACTTTGTGTCAAACGTTTTGACAGCAAGGAGTGATGTCTACGGATTTGGAGTGGTTCTATTGGAGCTTTTGACAGGGAAGAAGGCTCTGTTTAGAAGTAGTGAAGACGGAGGTCCAATGACGGTTGTGGAATACGTAGGGCCGCGAATAATGGCAGGGGAGCTGCAAAGCATGTTGGATAAAAGGGTGGGGCAGCCCGACCCAAATGAAGGCGAAGCAGTTGAGCTCTTGGCTTATACTGCTATGCGTTGTGTGAACTTGGAGGGGAAGGGAAGGCCTAGCATGACTGACATTGTAGCCAATTTAGAGAGGGCTCTTGCTCTTTGCGAGGATGATCCTTTTAGCTTCTCTACCACCACAATTTCCCTCCCTTCAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase RCC1_2

Protein Analysis

802

Amino Acids

85.91

Weight (kDa)

7.17

Isoelectric Point (pI)

36.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RCC1_2	PF13540	212 - 239	8.2e-06	Regulator of chromosome condensation (RCC1) repeat
Pkinase	PF00069	494 - 712	1.5e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	495 - 777	3.1e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55950
fragaria_vesca	FvH4_3g38710 FvH4_7g24490
malus_domestica	MD00G1166500.v1.1 MD01G1153100.v1.1 MD03G1068800.v1.1 MD07G1222100.v1.1 MD11G1073300.v1.1
prunus_persica	Prupe.2G250300_v2.0.a1 Prupe.6G056600_v2.0.a1
pyrus_communis	pycom01g17070 pycom03g05510
rosa_chinensis	RchiOBHm_Chr1g0370251 RchiOBHm_Chr5g0065501 RchiOBHm_Chr5g0069861
rosa_laevigata	RLG00000027065 RLG00000036103
rosa_multiflora	Rmu_co8321277.1_g000001 Rmu_sc0000738.1_g000002 Rmu_sc0026893.1_g000001 Rmu_sc0037931.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011190 Rroxscaffold_4G00286710 Rroxscaffold_4G00287300
rosa_rugosa	Rorug01G0355200 Rorug05G0400300
rosa_samantha	Rh1AG363800 Rh1BG326900 Rh1CG340200 Rh1DG357300 Rh5AG457400 Rh5BG476200 Rh5CG500000 Rh5DG488000
rosa_wichuraiana	Rw1G032010 Rw5G042700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	319
Acc36I	ACCTGC	1 cut(s)	881
AccI	GTMKAC	1 cut(s)	2064
AccII	CGCG	4 cut(s)	15, 1613, 1817, 2170
AccIII	TCCGGA	1 cut(s)	521
AciI	CCGC	7 cut(s)	33, 36, 528, 648, 909, 1538, 2168
AclI	AACGTT	1 cut(s)	2040
AclWI	GGATC	8 cut(s)	658, 1219, 1316, 1671, 1684, 1972, 2012, 2354
AcoI	YGGCCR	3 cut(s)	93, 341, 1554
AcsI	RAATTY	5 cut(s)	775, 898, 1042, 1592, 1789
AcuI	CTGAAG	1 cut(s)	2382
AcyI	GRCGYC	1 cut(s)	99
AdeI	CACNNNGTG	1 cut(s)	1663
AfaI	GTAC	9 cut(s)	726, 736, 1024, 1037, 1302, 1579, 1718, 2012, 2027
AfiI	CCNNNNNNNGG	7 cut(s)	489, 528, 766, 1045, 1331, 1563, 2234
AflIII	ACRYGT	1 cut(s)	627
AhdI	GACNNNNNGTC	1 cut(s)	1888
AjnI	CCWGG	7 cut(s)	487, 949, 997, 1446, 1580, 1659, 1829
AjuI	GAANNNNNNNTTGG	2 cut(s)	668, 700
Alw21I	GWGCWC	1 cut(s)	2253
Alw26I	GTCTC	2 cut(s)	305, 1043
AlwI	GGATC	8 cut(s)	658, 1219, 1316, 1671, 1684, 1972, 2012, 2354
AlwNI	CAGNNNCTG	1 cut(s)	444
Ama87I	CYCGRG	1 cut(s)	2021
Aor13HI	TCCGGA	1 cut(s)	521
AoxI	GGCC	8 cut(s)	93, 274, 341, 757, 1554, 1958, 2165, 2300
ApeKI	GCWGC	5 cut(s)	1343, 1779, 1826, 2188, 2218
ApoI	RAATTY	5 cut(s)	775, 898, 1042, 1592, 1789
Asp700I	GAANNNNTTC	2 cut(s)	263, 1314
AspLEI	GCGC	3 cut(s)	413, 1613, 1819
AspS9I	GGNCC	6 cut(s)	947, 1001, 1016, 1958, 2138, 2165
AsuC2I	CCSGG	4 cut(s)	1091, 1155, 1642, 1962
AsuHPI	GGTGA	7 cut(s)	22, 407, 477, 1436, 1436, 1640, 1675
AvaI	CYCGRG	1 cut(s)	2021
AvaII	GGWCC	4 cut(s)	947, 1001, 1016, 2138
AxyI	CCTNAGG	1 cut(s)	278
BalI	TGGCCA	1 cut(s)	1556
BamHI	GGATCC	1 cut(s)	1676
BanII	GRGCYC	3 cut(s)	173, 2253, 2341
BarI	GAAGNNNNNNTAC	2 cut(s)	1830, 1862
BbsI	GAAGAC	1 cut(s)	2136
Bbv12I	GWGCWC	1 cut(s)	2253
BbvI	GCAGC	5 cut(s)	1330, 1791, 1838, 2175, 2230
BccI	CCATC	3 cut(s)	551, 1535, 1565
BciT130I	CCWGG	7 cut(s)	489, 951, 999, 1448, 1582, 1661, 1831
BclI	TGATCA	2 cut(s)	45, 1741
BcnI	CCSGG	4 cut(s)	1091, 1155, 1642, 1962
BcoDI	GTCTC	2 cut(s)	305, 1043
BfaI	CTAG	8 cut(s)	324, 414, 429, 960, 1026, 1347, 1670, 2304
BfmI	CTRYAG	2 cut(s)	691, 888
BfuAI	ACCTGC	1 cut(s)	881
BisI	GCNGC	8 cut(s)	36, 910, 1344, 1780, 1827, 2168, 2189, 2219
BlpI	GCTNAGC	1 cut(s)	109
BlsI	GCNGC	8 cut(s)	37, 911, 1345, 1781, 1828, 2169, 2190, 2220
BmcAI	AGTACT	2 cut(s)	736, 2027
Bme18I	GGWCC	4 cut(s)	947, 1001, 1016, 2138
BmeRI	GACNNNNNGTC	1 cut(s)	1888
BmeT110I	CYCGRG	1 cut(s)	2021
BmgT120I	GGNCC	6 cut(s)	947, 1001, 1016, 1958, 2138, 2165
BmiI	GGNNCC	4 cut(s)	948, 1017, 1678, 1959
BmrI	ACTGGG	1 cut(s)	992
BmsI	GCATC	2 cut(s)	1385, 1900
BmuI	ACTGGG	1 cut(s)	992
BoxI	GACNNNNGTC	1 cut(s)	2136
BpiI	GAAGAC	1 cut(s)	2136
BpmI	CTGGAG	3 cut(s)	273, 306, 466
Bpu1102I	GCTNAGC	1 cut(s)	109
BpuEI	CTTGAG	1 cut(s)	688
BpuMI	CCSGG	4 cut(s)	1091, 1155, 1642, 1962
BsaAI	YACGTR	1 cut(s)	2161
BsaBI	GATNNNNATC	1 cut(s)	1811
BsaHI	GRCGYC	1 cut(s)	99
BsaI	GGTCTC	1 cut(s)	305
BsaJI	CCNNGG	4 cut(s)	103, 488, 950, 1830
BsaWI	WCCGGW	1 cut(s)	521
Bsc4I	CCNNNNNNNGG	7 cut(s)	489, 528, 766, 1045, 1331, 1563, 2234
Bse1I	ACTGG	3 cut(s)	748, 987, 1267
Bse21I	CCTNAGG	1 cut(s)	278
Bse3DI	GCAATG	3 cut(s)	1115, 1631, 1789
Bse8I	GATNNNNATC	1 cut(s)	1811
BseAI	TCCGGA	1 cut(s)	521
BseBI	CCWGG	7 cut(s)	489, 951, 999, 1448, 1582, 1661, 1831
BseDI	CCNNGG	4 cut(s)	103, 488, 950, 1830
BseGI	GGATG	4 cut(s)	518, 1546, 1915, 2362
BseJI	GATNNNNATC	1 cut(s)	1811
BseLI	CCNNNNNNNGG	7 cut(s)	489, 528, 766, 1045, 1331, 1563, 2234
BseMI	GCAATG	3 cut(s)	1115, 1631, 1789
BseMII	CTCAG	3 cut(s)	156, 292, 588
BseNI	ACTGG	3 cut(s)	748, 987, 1267
BseX3I	CGGCCG	1 cut(s)	341
BseXI	GCAGC	5 cut(s)	1330, 1791, 1838, 2175, 2230
BseYI	CCCAGC	3 cut(s)	306, 819, 1502
BsgI	GTGCAG	3 cut(s)	1031, 1222, 1845
Bsh1236I	CGCG	4 cut(s)	15, 1613, 1817, 2170
Bsh1285I	CGRYCG	1 cut(s)	344
BshFI	GGCC	8 cut(s)	95, 276, 343, 759, 1556, 1960, 2167, 2302
BsiEI	CGRYCG	1 cut(s)	344
BsiHKAI	GWGCWC	1 cut(s)	2253
BsiHKCI	CYCGRG	1 cut(s)	2021
BsiSI	CCGG	5 cut(s)	522, 1090, 1154, 1642, 1961
BslFI	GGGAC	5 cut(s)	960, 1234, 1581, 1624, 1895
BslI	CCNNNNNNNGG	7 cut(s)	489, 528, 766, 1045, 1331, 1563, 2234
BsmAI	GTCTC	2 cut(s)	305, 1043
BsmFI	GGGAC	5 cut(s)	960, 1234, 1581, 1624, 1895
BsmI	GAATGC	1 cut(s)	1927
BsnI	GGCC	8 cut(s)	95, 276, 343, 759, 1556, 1960, 2167, 2302
Bso31I	GGTCTC	1 cut(s)	305
BsoBI	CYCGRG	1 cut(s)	2021
Bsp1286I	GDGCHC	3 cut(s)	173, 2253, 2341
Bsp13I	TCCGGA	1 cut(s)	521
Bsp1720I	GCTNAGC	1 cut(s)	109
BspACI	CCGC	7 cut(s)	33, 36, 528, 648, 909, 1538, 2168
BspANI	GGCC	8 cut(s)	95, 276, 343, 759, 1556, 1960, 2167, 2302
BspCNI	CTCAG	3 cut(s)	157, 291, 589
BspEI	TCCGGA	1 cut(s)	521
BspFNI	CGCG	4 cut(s)	15, 1613, 1817, 2170
BspHI	TCATGA	1 cut(s)	1738
BspLI	GGNNCC	4 cut(s)	948, 1017, 1678, 1959
BspMAI	CTGCAG	2 cut(s)	695, 892
BspMI	ACCTGC	1 cut(s)	881
BspPI	GGATC	8 cut(s)	658, 1219, 1316, 1671, 1684, 1972, 2012, 2354
BspQI	GCTCTTC	1 cut(s)	254
BspTNI	GGTCTC	1 cut(s)	305
BsrDI	GCAATG	3 cut(s)	1115, 1631, 1789
BsrI	ACTGG	3 cut(s)	748, 987, 1267
BssECI	CCNNGG	4 cut(s)	103, 488, 950, 1830
BssNI	GRCGYC	1 cut(s)	99
BssT1I	CCWWGG	1 cut(s)	103
Bst2UI	CCWGG	7 cut(s)	489, 951, 999, 1448, 1582, 1661, 1831
Bst4CI	ACNGT	8 cut(s)	148, 189, 203, 362, 851, 1730, 1947, 2149
Bst6I	CTCTTC	2 cut(s)	254, 448
BstACI	GRCGYC	1 cut(s)	99
BstAPI	GCANNNNNTGC	2 cut(s)	189, 1086
BstBAI	YACGTR	1 cut(s)	2161
BstC8I	GCNNGC	3 cut(s)	646, 1532, 1536
BstDEI	CTNAG	4 cut(s)	109, 165, 278, 597
BstF5I	GGATG	4 cut(s)	518, 1546, 1915, 2362
BstFNI	CGCG	4 cut(s)	15, 1613, 1817, 2170
BstHHI	GCGC	3 cut(s)	413, 1613, 1819
BstMAI	GTCTC	2 cut(s)	305, 1043
BstMCI	CGRYCG	1 cut(s)	344
BstMWI	GCNNNNNNNGC	8 cut(s)	189, 324, 770, 1086, 1352, 1642, 1823, 2264
BstNI	CCWGG	7 cut(s)	489, 951, 999, 1448, 1582, 1661, 1831
BstNSI	RCATGY	2 cut(s)	631, 2200
BstPAI	GACNNNNGTC	1 cut(s)	2136
BstSFI	CTRYAG	2 cut(s)	691, 888
BstSNI	TACGTA	1 cut(s)	2161
BstUI	CGCG	4 cut(s)	15, 1613, 1817, 2170
BstV1I	GCAGC	5 cut(s)	1330, 1791, 1838, 2175, 2230
BstV2I	GAAGAC	1 cut(s)	2136
BstX2I	RGATCY	2 cut(s)	650, 1676
BstXI	CCANNNNNNTGG	1 cut(s)	84
BstYI	RGATCY	2 cut(s)	650, 1676
BstZI	CGGCCG	1 cut(s)	341
Bsu36I	CCTNAGG	1 cut(s)	278
BsuRI	GGCC	8 cut(s)	95, 276, 343, 759, 1556, 1960, 2167, 2302
BtsCI	GGATG	4 cut(s)	518, 1546, 1915, 2362
BtsI	GCAGTG	2 cut(s)	1461, 1866
BtsIMutI	CAGTG	8 cut(s)	194, 367, 856, 939, 980, 1461, 1866, 2406
BveI	ACCTGC	1 cut(s)	881
Cac8I	GCNNGC	3 cut(s)	646, 1532, 1536
CaiI	CAGNNNCTG	1 cut(s)	444
CciI	TCATGA	1 cut(s)	1738
CfoI	GCGC	3 cut(s)	413, 1613, 1819
Cfr13I	GGNCC	6 cut(s)	947, 1001, 1016, 1958, 2138, 2165
CseI	GACGC	1 cut(s)	107
CsiI	ACCWGGT	2 cut(s)	1446, 1659
Csp6I	GTAC	9 cut(s)	725, 735, 1023, 1036, 1301, 1578, 1717, 2011, 2026
CviQI	GTAC	9 cut(s)	725, 735, 1023, 1036, 1301, 1578, 1717, 2011, 2026
DdeI	CTNAG	4 cut(s)	109, 165, 278, 597
DraIII	CACNNNGTG	1 cut(s)	1663
DrdI	GACNNNNNNGTC	1 cut(s)	319
DriI	GACNNNNNGTC	1 cut(s)	1888
DseDI	GACNNNNNNGTC	1 cut(s)	319
EaeI	YGGCCR	3 cut(s)	93, 341, 1554
EagI	CGGCCG	1 cut(s)	341
Eam1104I	CTCTTC	2 cut(s)	254, 448
Eam1105I	GACNNNNNGTC	1 cut(s)	1888
EarI	CTCTTC	2 cut(s)	254, 448
EciI	GGCGGA	1 cut(s)	663
Ecl136II	GAGCTC	1 cut(s)	2251
EclXI	CGGCCG	1 cut(s)	341
Eco105I	TACGTA	1 cut(s)	2161
Eco130I	CCWWGG	1 cut(s)	103
Eco147I	AGGCCT	2 cut(s)	759, 2302
Eco24I	GRGCYC	3 cut(s)	173, 2253, 2341
Eco31I	GGTCTC	1 cut(s)	305
Eco47I	GGWCC	4 cut(s)	947, 1001, 1016, 2138
Eco52I	CGGCCG	1 cut(s)	341
Eco53kI	GAGCTC	1 cut(s)	2251
Eco57I	CTGAAG	1 cut(s)	2382
Eco81I	CCTNAGG	1 cut(s)	278
Eco88I	CYCGRG	1 cut(s)	2021
EcoICRI	GAGCTC	1 cut(s)	2251
EcoRI	GAATTC	2 cut(s)	1042, 1789
EcoRII	CCWGG	7 cut(s)	487, 949, 997, 1446, 1580, 1659, 1829
EcoT14I	CCWWGG	1 cut(s)	103
EcoT38I	GRGCYC	3 cut(s)	173, 2253, 2341
ErhI	CCWWGG	1 cut(s)	103
FalI	AAGNNNNNCTT	6 cut(s)	251, 283, 358, 390, 1518, 1550
FaqI	GGGAC	5 cut(s)	960, 1234, 1581, 1624, 1895
FauI	CCCGC	1 cut(s)	521
FauNDI	CATATG	1 cut(s)	133
FbaI	TGATCA	2 cut(s)	45, 1741
FblI	GTMKAC	1 cut(s)	2064
Fnu4HI	GCNGC	8 cut(s)	36, 910, 1344, 1780, 1827, 2168, 2189, 2219
FokI	GGATG	4 cut(s)	505, 1553, 1922, 2369
FriOI	GRGCYC	3 cut(s)	173, 2253, 2341
Fsp4HI	GCNGC	8 cut(s)	36, 910, 1344, 1780, 1827, 2168, 2189, 2219
FspBI	CTAG	8 cut(s)	324, 414, 429, 960, 1026, 1347, 1670, 2304
GlaI	GCGC	3 cut(s)	412, 1612, 1818
GluI	GCNGC	8 cut(s)	36, 910, 1344, 1780, 1827, 2168, 2189, 2219
GsaI	CCCAGC	3 cut(s)	310, 823, 1506
GsuI	CTGGAG	3 cut(s)	273, 306, 466
HaeIII	GGCC	8 cut(s)	95, 276, 343, 759, 1556, 1960, 2167, 2302
HapII	CCGG	5 cut(s)	522, 1090, 1154, 1642, 1961
HgaI	GACGC	1 cut(s)	107
HhaI	GCGC	3 cut(s)	413, 1613, 1819
Hin1I	GRCGYC	1 cut(s)	99
Hin6I	GCGC	3 cut(s)	411, 1611, 1817
HinP1I	GCGC	3 cut(s)	411, 1611, 1817
HincII	GTYRAC	1 cut(s)	424
HindII	GTYRAC	1 cut(s)	424
HindIII	AAGCTT	2 cut(s)	1143, 1532
HinfI	GANTC	9 cut(s)	601, 807, 1051, 1233, 1266, 1295, 1350, 1436, 1619
HpaII	CCGG	5 cut(s)	522, 1090, 1154, 1642, 1961
HphI	GGTGA	7 cut(s)	22, 407, 477, 1436, 1436, 1640, 1675
Hpy166II	GTNNAC	7 cut(s)	424, 625, 1036, 1711, 1733, 2065, 2281
Hpy188I	TCNGA	5 cut(s)	144, 207, 1048, 1624, 1936
Hpy8I	GTNNAC	7 cut(s)	424, 625, 1036, 1711, 1733, 2065, 2281
Hpy99I	CGWCG	4 cut(s)	101, 148, 1417, 1697
HpyCH4III	ACNGT	8 cut(s)	148, 189, 203, 362, 851, 1730, 1947, 2149
HpyCH4IV	ACGT	2 cut(s)	2040, 2160
HpyF10VI	GCNNNNNNNGC	8 cut(s)	189, 324, 770, 1086, 1352, 1642, 1823, 2264
HpyF3I	CTNAG	4 cut(s)	109, 165, 278, 597
HpySE526I	ACGT	2 cut(s)	2040, 2160
Hsp92I	GRCGYC	1 cut(s)	99
HspAI	GCGC	3 cut(s)	411, 1611, 1817
Kpn2I	TCCGGA	1 cut(s)	521
Ksp22I	TGATCA	2 cut(s)	45, 1741
LguI	GCTCTTC	1 cut(s)	254
LmnI	GCTCC	4 cut(s)	593, 1506, 2089, 2185
Lsp1109I	GCAGC	5 cut(s)	1330, 1791, 1838, 2175, 2230
LweI	GCATC	2 cut(s)	1385, 1900
MabI	ACCWGGT	2 cut(s)	1446, 1659
MaeI	CTAG	8 cut(s)	324, 414, 429, 960, 1026, 1347, 1670, 2304
MaeII	ACGT	2 cut(s)	2040, 2160
MaeIII	GTNAC	2 cut(s)	913, 1424
MboII	GAAGA	9 cut(s)	54, 259, 271, 465, 840, 968, 1838, 2116, 2141
MfeI	CAATTG	2 cut(s)	716, 2004
MflI	RGATCY	2 cut(s)	650, 1676
MhlI	GDGCHC	3 cut(s)	173, 2253, 2341
MlsI	TGGCCA	1 cut(s)	1556
MluNI	TGGCCA	1 cut(s)	1556
MlyI	GAGTC	7 cut(s)	1045, 1227, 1260, 1289, 1359, 1445, 1613
MmeI	TCCRAC	6 cut(s)	167, 247, 571, 1189, 1978, 2181
Mox20I	TGGCCA	1 cut(s)	1556
MroI	TCCGGA	1 cut(s)	521
MroXI	GAANNNNTTC	2 cut(s)	263, 1314
MscI	TGGCCA	1 cut(s)	1556
MseI	TTAA	3 cut(s)	881, 1283, 1400
MslI	CAYNNNNRTG	2 cut(s)	660, 1854
Msp20I	TGGCCA	1 cut(s)	1556
MspI	CCGG	5 cut(s)	522, 1090, 1154, 1642, 1961
MunI	CAATTG	2 cut(s)	716, 2004
Mva1269I	GAATGC	1 cut(s)	1927
MvaI	CCWGG	7 cut(s)	489, 951, 999, 1448, 1582, 1661, 1831
MvnI	CGCG	4 cut(s)	15, 1613, 1817, 2170
MwoI	GCNNNNNNNGC	8 cut(s)	189, 324, 770, 1086, 1352, 1642, 1823, 2264
NciI	CCSGG	4 cut(s)	1091, 1155, 1642, 1962
NdeI	CATATG	1 cut(s)	133
NlaIV	GGNNCC	4 cut(s)	948, 1017, 1678, 1959
NmuCI	GTSAC	2 cut(s)	913, 1424
NspI	RCATGY	2 cut(s)	631, 2200
PagI	TCATGA	1 cut(s)	1738
PceI	AGGCCT	2 cut(s)	759, 2302
PciI	ACATGT	1 cut(s)	627
PciSI	GCTCTTC	1 cut(s)	254
PcsI	WCGNNNNNNNCGW	1 cut(s)	909
PctI	GAATGC	1 cut(s)	1927
PdmI	GAANNNNTTC	2 cut(s)	263, 1314
PfeI	GAWTC	2 cut(s)	601, 807
PkrI	GCNGC	8 cut(s)	37, 911, 1345, 1781, 1828, 2169, 2190, 2220
PleI	GAGTC	7 cut(s)	1045, 1227, 1260, 1289, 1358, 1444, 1613
PpsI	GAGTC	7 cut(s)	1045, 1227, 1260, 1289, 1358, 1444, 1613
Ppu21I	YACGTR	1 cut(s)	2161
PscI	ACATGT	1 cut(s)	627
PshAI	GACNNNNGTC	1 cut(s)	2136
Psp124BI	GAGCTC	1 cut(s)	2253
Psp1406I	AACGTT	1 cut(s)	2040
Psp6I	CCWGG	7 cut(s)	487, 949, 997, 1446, 1580, 1659, 1829
PspFI	CCCAGC	3 cut(s)	306, 819, 1502
PspGI	CCWGG	7 cut(s)	487, 949, 997, 1446, 1580, 1659, 1829
PspN4I	GGNNCC	4 cut(s)	948, 1017, 1678, 1959
PspPI	GGNCC	6 cut(s)	947, 1001, 1016, 1958, 2138, 2165
PstI	CTGCAG	2 cut(s)	695, 892
PstNI	CAGNNNCTG	1 cut(s)	444
PsuI	RGATCY	2 cut(s)	650, 1676
RsaI	GTAC	9 cut(s)	726, 736, 1024, 1037, 1302, 1579, 1718, 2012, 2027
RsaNI	GTAC	9 cut(s)	725, 735, 1023, 1036, 1301, 1578, 1717, 2011, 2026
RseI	CAYNNNNRTG	2 cut(s)	660, 1854
SacI	GAGCTC	1 cut(s)	2253
SapI	GCTCTTC	1 cut(s)	254
SaqAI	TTAA	3 cut(s)	881, 1283, 1400
SatI	GCNGC	8 cut(s)	36, 910, 1344, 1780, 1827, 2168, 2189, 2219
Sau96I	GGNCC	6 cut(s)	947, 1001, 1016, 1958, 2138, 2165
ScaI	AGTACT	2 cut(s)	736, 2027
SchI	GAGTC	7 cut(s)	1045, 1227, 1260, 1289, 1359, 1445, 1613
SduI	GDGCHC	3 cut(s)	173, 2253, 2341
SexAI	ACCWGGT	2 cut(s)	1446, 1659
SfaNI	GCATC	2 cut(s)	1385, 1900
SfcI	CTRYAG	2 cut(s)	691, 888
SinI	GGWCC	4 cut(s)	947, 1001, 1016, 2138
SmiMI	CAYNNNNRTG	2 cut(s)	660, 1854
SmlI	CTYRAG	1 cut(s)	667
SmoI	CTYRAG	1 cut(s)	667
SnaBI	TACGTA	1 cut(s)	2161
SseBI	AGGCCT	2 cut(s)	759, 2302
SsiI	CCGC	7 cut(s)	33, 36, 528, 648, 909, 1538, 2168
SspI	AATATT	2 cut(s)	232, 1475
SspMI	CTAG	8 cut(s)	324, 414, 429, 960, 1026, 1347, 1670, 2304
SstI	GAGCTC	1 cut(s)	2253
StuI	AGGCCT	2 cut(s)	759, 2302
StyI	CCWWGG	1 cut(s)	103
TaaI	ACNGT	8 cut(s)	148, 189, 203, 362, 851, 1730, 1947, 2149
TaiI	ACGT	2 cut(s)	2043, 2163
TaqI	TCGA	2 cut(s)	740, 1415
TatI	WGTACW	4 cut(s)	734, 1022, 1716, 2025
TauI	GCSGC	3 cut(s)	38, 912, 2170
TfiI	GAWTC	2 cut(s)	601, 807
Tru1I	TTAA	3 cut(s)	881, 1283, 1400
Tru9I	TTAA	3 cut(s)	881, 1283, 1400
TscAI	CASTG	8 cut(s)	194, 367, 856, 939, 987, 1468, 1866, 2406
TseFI	GTSAC	2 cut(s)	913, 1424
TseI	GCWGC	5 cut(s)	1343, 1779, 1826, 2188, 2218
Tsp45I	GTSAC	2 cut(s)	913, 1424
TspDTI	ATGAA	8 cut(s)	46, 689, 861, 1359, 1398, 1403, 1727, 2247
TspGWI	ACGGA	3 cut(s)	1273, 2082, 2148
TspRI	CASTG	8 cut(s)	194, 367, 856, 939, 987, 1468, 1866, 2406
VpaK11BI	GGWCC	4 cut(s)	947, 1001, 1016, 2138
XapI	RAATTY	5 cut(s)	775, 898, 1042, 1592, 1789
XbaI	TCTAGA	1 cut(s)	428
XceI	RCATGY	2 cut(s)	631, 2200
XcmI	CCANNNNNNNNNTGG	1 cut(s)	84
XmiI	GTMKAC	1 cut(s)	2064
XmnI	GAANNNNTTC	2 cut(s)	263, 1314
XspI	CTAG	8 cut(s)	324, 414, 429, 960, 1026, 1347, 1670, 2304
ZrmI	AGTACT	2 cut(s)	736, 2027

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.