Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0003366

Physical Location & Seq

Genomic Coordinates Reverse (-)

74676564 .. 74678864

Gene Structure 2301 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G042700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2301 bp

ATGACACTTGTATATGTCTTAATCTTCCTCACAACCTTCATATCTTCGCTGCCACCCAGCAGTGTGGTTGTCAATGGCCTAGGATTAGCATCCACCACTGCCATAGCATATGGCACGTCAACTATTTGTGGCGTTGTAGCTAACCACCAGACGCAGTTCATACAATGCTACCAAAACAACCGGACTATTTTAGTCCAACCCTCTGTCTCTTTTGAAGCAATATCTGGTGGGAAGACCTTCTTCTGTGGTCTCCGGTCCGGTGGCTTTAGCCTTCTTTGCTGGGACACCAGCTCTGTTAGCTTCAATCCTAGACGTATATACCACAGTGACGACGTAGCGTTGACTGATCTTACGGTTGGTGATGCTCAAGTTTGCGCCAGAGAGGTCAACTCTAGCACAGTCAGGTGCTGGAGAGGAGGGGCTTTGTTTCACTCACCAGGGGAGGCATTGAAGTTCAAGACAGTGACATCTGGAAGTGGGTTTACATGTGGGATTTTGATGAATGGTAGCAGAGTCCTCTGTTGGGGAAATGGTATTGGAGCTGAGATTCAAAAAGGGTTCGAGAATGTGTTAATGTCAAGCTTAATTGCTGGAGAGTCTCATGCTTGTGGCTTGAGGAAAAATGGAACTTTGGTCTGCAAAGGGAACAATGATTCCGGGCAATTGAATGTACCTTATGGGGCCAAGTTTTCAGGCCTTGCATTGGGAGCAAATTTTACTTGTGCTATAAGGCAAAGCAATGGCTATGTTGTATGCTGGGGTCACAGTGATGTTGTTGGAAATGTTTCGTTTGAGTCGATTGTTGCAGGTTTGGATTTTGTATGTGGACTAACAAGAGGTAATCTATCAGTGATTTGTTGGGGACCAGGATGGTCTAGTTCTGAAAATGATGTTACTTTGGGATCGATCATTCCGGGTCCATGTGTACAAACTCCTTGTAGCAGCTGTGGTGTGTACCCAAATTCGGAGACTCTTTGTGGTGGGTCGGGGAATATATGCAACTCATGCCAGACCGAGCTTCCAGTTGCAGTGCCTTTGCTTCCAACAAGTCCACTGGAAAAAGGTTCTTCATCATCCATAGGAAAAAATAAGCTTTTGTTGGTTTTTGTAATAGTTGGATCAGTTGGGGCTTTTGCTGGACTTTGCACTGTTCTTTTCTGCTTATGGATTGGACTGTGCAGCTCTTGGTTCAACAACCGGGAGTCTGAGCAACTCACAAGTGCTGCTGACCCCAATGTAGGGGCTCTGGTTGAGATCCAAAACGTTCCTAATGCTCCACTTCAGCACTTTAGTAGTAGTTCATCATCAAAGCATGCAGACAAGACTGAAGAATTTGTATTAGCAGAGCTTTCTGCTGCCACTAAGCATTTCTCAACCCAAAACAAGATTGGTGCCGGGAGCTTTGGTATTGTATACAGAGGCAAGCTCTCCGATGGTCGCGAAGTGGCCATCAAGAGAGGAGATACTAGTACAAAAACCAAGAAATTCCAGGAGAAAGAAAGCGCGTTTGATTCTGAAGTAGCATTGCTGTCTCGGCTTCACCACAAGCATTTGGTGAAGCTAGTGGGATCGTGTGAAGATAAAGATGAGAGGCTTTTGGTTTACGAGTACATGAGTAATGGCTCGCTTCATGATCACTTGCACAACAAGAAAAATGTGGAGAAAAGTAGCAGCATTGTGAACTCTTGGAAAATGAGGATGAGAATAGCATTGGATGCTGCAAGGGGGATTGAGTATCTTCACACTTATGCAGTGCCGCCAATAATTCATCGAGACATCAAGTCCTCTAACATTCTCTTGGATGCAGACATGACTGCAAGAGTATCTGATTTCGGGCTGTCATTGTTGGGGCCGGCATCTGATCAAGAGGTAATGTCATCAAAGGCCGTTGGGACAGTAGGGTACATTGATCCAGAGTACTTTGTACTAAACGTTTTGACTGCAAAGAGCGATGTCTATGGCTTCGGAGTGGTGTTGTTGGAGCTTTTGACAGGGAAGAGGGCTGTTTTCAGTGACAGTGAAGAAGGAACAGGTCCAATTGGTGTTGTGGAATATGCAGGGCCAAGGATAATGGCAGGAGATCTGCAAAGCTTGTTGGATAAAAGGCTTGGGCAGCCGGAGCCAAATGAGGCCGAAGCAGTTCAGCTAGTAGCTAATACTGCTATGCATTGTCTGAGTTTAGAGGGGAAAGAAAGGCCTAGTATGACTGACATTGTTGCCAATTTGGAGAGAGCAATTGCCCTTTGTGAACAGGAACCTGTTAGCTTCTCTACCTCCGCAATTTCCCTTCCTTCACTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase RCC1_2

Protein Analysis

766

Amino Acids

81.7

Weight (kDa)

6.05

Isoelectric Point (pI)

36.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RCC1_2	PF13540	197 - 221	2.7e-06	Regulator of chromosome condensation (RCC1) repeat
Pkinase	PF00069	458 - 740	2.7e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	459 - 742	4.4e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55950
fragaria_vesca	FvH4_3g38710 FvH4_7g24490
malus_domestica	MD00G1166500.v1.1 MD01G1153100.v1.1 MD03G1068800.v1.1 MD07G1222100.v1.1 MD11G1073300.v1.1
prunus_persica	Prupe.2G250300_v2.0.a1 Prupe.6G056600_v2.0.a1
pyrus_communis	pycom01g17070 pycom03g05510
rosa_chinensis	RchiOBHm_Chr1g0370251 RchiOBHm_Chr5g0065501 RchiOBHm_Chr5g0069861
rosa_laevigata	RLG00000027065 RLG00000036103
rosa_multiflora	Rmu_co8321277.1_g000001 Rmu_sc0000738.1_g000002 Rmu_sc0026893.1_g000001 Rmu_sc0037931.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011190 Rroxscaffold_4G00286710 Rroxscaffold_4G00287300
rosa_rugosa	Rorug01G0355200 Rorug05G0400300
rosa_samantha	Rh1AG363800 Rh1BG326900 Rh1CG340200 Rh1DG357300 Rh5AG457400 Rh5BG476200 Rh5CG500000 Rh5DG488000
rosa_wichuraiana	Rw1G032010 Rw5G042700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	797
AccB1I	GGYRCC	1 cut(s)	1391
AccI	GTMKAC	1 cut(s)	1413
AccII	CGCG	2 cut(s)	1440, 1505
AciI	CCGC	2 cut(s)	1757, 2277
AclI	AACGTT	2 cut(s)	1263, 1932
AclWI	GGATC	5 cut(s)	910, 1126, 1249, 1576, 1904
AcoI	YGGCCR	1 cut(s)	1446
AcsI	RAATTY	4 cut(s)	712, 961, 1331, 1484
AcuI	CTGAAG	3 cut(s)	1265, 1347, 1536
AfaI	GTAC	8 cut(s)	672, 927, 956, 1471, 1610, 1904, 1919, 1926
AfiI	CCNNNNNNNGG	5 cut(s)	523, 703, 964, 1238, 1239
AflIII	ACRYGT	1 cut(s)	485
AgsI	TTSAA	7 cut(s)	215, 304, 451, 457, 551, 667, 1192
AhdI	GACNNNNNGTC	1 cut(s)	1780
AhlI	ACTAGT	1 cut(s)	1466
AjiI	CACGTC	1 cut(s)	117
AjnI	CCWGG	3 cut(s)	436, 865, 1488
AloI	GAACNNNNNNTCC	2 cut(s)	638, 670
Alw26I	GTCTC	6 cut(s)	211, 254, 603, 962, 1536, 1767
AlwI	GGATC	5 cut(s)	910, 1126, 1249, 1576, 1904
AlwNI	CAGNNNCTG	1 cut(s)	408
AoxI	GGCC	9 cut(s)	76, 681, 694, 1446, 1850, 1884, 2060, 2130, 2195
ApeKI	GCWGC	8 cut(s)	49, 942, 1179, 1223, 1355, 1671, 1718, 2113
ApoI	RAATTY	4 cut(s)	712, 961, 1331, 1484
Asp700I	GAANNNNTTC	3 cut(s)	236, 784, 2139
AspA2I	CCTAGG	1 cut(s)	79
AspLEI	GCGC	2 cut(s)	377, 1505
AspS9I	GGNCC	7 cut(s)	255, 681, 863, 917, 1850, 2033, 2060
AsuC2I	CCSGG	4 cut(s)	658, 915, 1199, 1396
AsuHPI	GGTGA	4 cut(s)	371, 426, 1532, 1567
AvaII	GGWCC	4 cut(s)	255, 863, 917, 2033
AvrII	CCTAGG	1 cut(s)	79
BalI	TGGCCA	1 cut(s)	1448
BanI	GGYRCC	1 cut(s)	1391
BanII	GRGCYC	1 cut(s)	1246
BarI	GAAGNNNNNNTAC	2 cut(s)	466, 498
BbsI	GAAGAC	1 cut(s)	239
BbvI	GCAGC	8 cut(s)	36, 954, 1191, 1210, 1342, 1683, 1705, 2125
BccI	CCATC	3 cut(s)	864, 1427, 1457
BceAI	ACGGC	1 cut(s)	1871
BciT130I	CCWGG	3 cut(s)	438, 867, 1490
BclI	TGATCA	2 cut(s)	1633, 1861
BcnI	CCSGG	4 cut(s)	658, 915, 1199, 1396
BcoDI	GTCTC	6 cut(s)	211, 254, 603, 962, 1536, 1767
BcuI	ACTAGT	1 cut(s)	1466
BfaI	CTAG	8 cut(s)	80, 309, 393, 876, 1467, 1562, 2147, 2199
BfuAI	ACCTGC	1 cut(s)	797
BglII	AGATCT	1 cut(s)	2080
BisI	GCNGC	9 cut(s)	50, 943, 1180, 1224, 1356, 1672, 1719, 1757, 2114
BlnI	CCTAGG	1 cut(s)	79
BlsI	GCNGC	9 cut(s)	51, 944, 1181, 1225, 1357, 1673, 1720, 1758, 2115
BmcAI	AGTACT	1 cut(s)	1919
Bme1390I	CCNGG	7 cut(s)	438, 658, 867, 915, 1199, 1396, 1490
Bme18I	GGWCC	4 cut(s)	255, 863, 917, 2033
BmeRI	GACNNNNNGTC	1 cut(s)	1780
BmgBI	CACGTC	1 cut(s)	117
BmgT120I	GGNCC	7 cut(s)	255, 681, 863, 917, 1850, 2033, 2060
BmiI	GGNNCC	7 cut(s)	682, 864, 918, 1393, 1851, 2121, 2256
BmrFI	CCNGG	7 cut(s)	438, 658, 867, 915, 1199, 1396, 1490
BmsI	GCATC	5 cut(s)	98, 352, 1705, 1792, 1865
BpiI	GAAGAC	1 cut(s)	239
BplI	GAGNNNNNCTC	4 cut(s)	374, 406, 1410, 1442
BpmI	CTGGAG	2 cut(s)	430, 612
BpuEI	CTTGAG	2 cut(s)	351, 634
BpuMI	CCSGG	4 cut(s)	658, 915, 1199, 1396
Bsa29I	ATCGAT	1 cut(s)	905
BsaBI	GATNNNNATC	1 cut(s)	88
BsaI	GGTCTC	1 cut(s)	254
BsaJI	CCNNGG	3 cut(s)	79, 437, 2063
BsaWI	WCCGGW	3 cut(s)	180, 252, 257
BsaXI	ACNNNNNCTCC	2 cut(s)	1390, 1420
Bsc4I	CCNNNNNNNGG	5 cut(s)	523, 703, 964, 1238, 1239
Bse118I	RCCGGY	1 cut(s)	1852
Bse1I	ACTGG	2 cut(s)	1022, 1059
Bse3DI	GCAATG	2 cut(s)	745, 1523
Bse8I	GATNNNNATC	1 cut(s)	88
BseBI	CCWGG	3 cut(s)	438, 867, 1490
BseCI	ATCGAT	1 cut(s)	905
BseDI	CCNNGG	3 cut(s)	79, 437, 2063
BseGI	GGATG	6 cut(s)	89, 875, 1073, 1704, 1720, 1807
BseJI	GATNNNNATC	1 cut(s)	88
BseLI	CCNNNNNNNGG	5 cut(s)	523, 703, 964, 1238, 1239
BseMI	GCAATG	2 cut(s)	745, 1523
BseMII	CTCAG	3 cut(s)	534, 1197, 2165
BseNI	ACTGG	2 cut(s)	1022, 1059
BseRI	GAGGAG	2 cut(s)	429, 1473
BseXI	GCAGC	8 cut(s)	36, 954, 1191, 1210, 1342, 1683, 1705, 2125
BseYI	CCCAGC	3 cut(s)	56, 279, 756
BsgI	GTGCAG	1 cut(s)	1198
Bsh1236I	CGCG	2 cut(s)	1440, 1505
BshFI	GGCC	9 cut(s)	78, 683, 696, 1448, 1852, 1886, 2062, 2132, 2197
BshNI	GGYRCC	1 cut(s)	1391
BshVI	ATCGAT	1 cut(s)	905
BsiSI	CCGG	9 cut(s)	181, 253, 258, 657, 914, 1198, 1395, 1853, 2117
BslFI	GGGAC	3 cut(s)	296, 876, 1906
BslI	CCNNNNNNNGG	5 cut(s)	523, 703, 964, 1238, 1239
BsmAI	GTCTC	6 cut(s)	211, 254, 603, 962, 1536, 1767
BsmFI	GGGAC	3 cut(s)	296, 876, 1906
BsnI	GGCC	9 cut(s)	78, 683, 696, 1448, 1852, 1886, 2062, 2132, 2197
Bso31I	GGTCTC	1 cut(s)	254
Bsp1286I	GDGCHC	1 cut(s)	1246
Bsp1407I	TGTACA	1 cut(s)	925
Bsp68I	TCGCGA	1 cut(s)	1440
BspACI	CCGC	2 cut(s)	1757, 2277
BspANI	GGCC	9 cut(s)	78, 683, 696, 1448, 1852, 1886, 2062, 2132, 2197
BspCNI	CTCAG	3 cut(s)	535, 1198, 2166
BspDI	ATCGAT	1 cut(s)	905
BspFNI	CGCG	2 cut(s)	1440, 1505
BspHI	TCATGA	1 cut(s)	1630
BspLI	GGNNCC	7 cut(s)	682, 864, 918, 1393, 1851, 2121, 2256
BspMI	ACCTGC	1 cut(s)	797
BspPI	GGATC	5 cut(s)	910, 1126, 1249, 1576, 1904
BspT107I	GGYRCC	1 cut(s)	1391
BspTNI	GGTCTC	1 cut(s)	254
BsrDI	GCAATG	2 cut(s)	745, 1523
BsrFI	RCCGGY	1 cut(s)	1852
BsrGI	TGTACA	1 cut(s)	925
BsrI	ACTGG	2 cut(s)	1022, 1059
BssAI	RCCGGY	1 cut(s)	1852
BssECI	CCNNGG	3 cut(s)	79, 437, 2063
BssNAI	GTATAC	1 cut(s)	1414
BssT1I	CCWWGG	2 cut(s)	79, 2063
Bst1107I	GTATAC	1 cut(s)	1414
Bst2UI	CCWGG	3 cut(s)	438, 867, 1490
Bst6I	CTCTTC	1 cut(s)	1991
BstAPI	GCANNNNNTGC	2 cut(s)	1005, 1715
BstAUI	TGTACA	1 cut(s)	925
BstC8I	GCNNGC	4 cut(s)	1314, 1424, 1625, 1854
BstDEI	CTNAG	4 cut(s)	543, 1206, 1362, 2174
BstF5I	GGATG	6 cut(s)	89, 875, 1073, 1704, 1720, 1807
BstFNI	CGCG	2 cut(s)	1440, 1505
BstHHI	GCGC	2 cut(s)	377, 1505
BstMAI	GTCTC	6 cut(s)	211, 254, 603, 962, 1536, 1767
BstMWI	GCNNNNNNNGC	9 cut(s)	276, 297, 707, 1005, 1534, 1715, 2113, 2119, 2159
BstNI	CCWGG	3 cut(s)	438, 867, 1490
BstNSI	RCATGY	2 cut(s)	489, 1316
BstSCI	CCNGG	7 cut(s)	436, 656, 865, 913, 1197, 1394, 1488
BstUI	CGCG	2 cut(s)	1440, 1505
BstV1I	GCAGC	8 cut(s)	36, 954, 1191, 1210, 1342, 1683, 1705, 2125
BstV2I	GAAGAC	1 cut(s)	239
BstX2I	RGATCY	2 cut(s)	1254, 2080
BstXI	CCANNNNNNTGG	1 cut(s)	64
BstYI	RGATCY	2 cut(s)	1254, 2080
BstZ17I	GTATAC	1 cut(s)	1414
Bsu15I	ATCGAT	1 cut(s)	905
BsuRI	GGCC	9 cut(s)	78, 683, 696, 1448, 1852, 1886, 2062, 2132, 2197
BsuTUI	ATCGAT	1 cut(s)	905
BtgZI	GCGATG	1 cut(s)	1965
BtrI	CACGTC	1 cut(s)	117
BtsCI	GGATG	6 cut(s)	89, 875, 1073, 1704, 1720, 1807
BtsI	GCAGTG	4 cut(s)	67, 96, 1035, 1758
BtuMI	TCGCGA	1 cut(s)	1440
BveI	ACCTGC	1 cut(s)	797
Cac8I	GCNNGC	4 cut(s)	1314, 1424, 1625, 1854
CaiI	CAGNNNCTG	1 cut(s)	408
CciI	TCATGA	1 cut(s)	1630
CfoI	GCGC	2 cut(s)	377, 1505
Cfr10I	RCCGGY	1 cut(s)	1852
Cfr13I	GGNCC	7 cut(s)	255, 681, 863, 917, 1850, 2033, 2060
ClaI	ATCGAT	1 cut(s)	905
CpoI	CGGWCCG	1 cut(s)	255
CseI	GACGC	1 cut(s)	160
Csp6I	GTAC	8 cut(s)	671, 926, 955, 1470, 1609, 1903, 1918, 1925
CspI	CGGWCCG	1 cut(s)	255
CviAII	CATG	8 cut(s)	486, 602, 921, 1005, 1313, 1612, 1631, 1810
CviQI	GTAC	8 cut(s)	671, 926, 955, 1470, 1609, 1903, 1918, 1925
DdeI	CTNAG	4 cut(s)	543, 1206, 1362, 2174
DriI	GACNNNNNGTC	1 cut(s)	1780
EaeI	YGGCCR	1 cut(s)	1446
Eam1104I	CTCTTC	1 cut(s)	1991
Eam1105I	GACNNNNNGTC	1 cut(s)	1780
EarI	CTCTTC	1 cut(s)	1991
Eco130I	CCWWGG	2 cut(s)	79, 2063
Eco147I	AGGCCT	2 cut(s)	696, 2197
Eco24I	GRGCYC	1 cut(s)	1246
Eco31I	GGTCTC	1 cut(s)	254
Eco47I	GGWCC	4 cut(s)	255, 863, 917, 2033
Eco57I	CTGAAG	3 cut(s)	1265, 1347, 1536
EcoRII	CCWGG	3 cut(s)	436, 865, 1488
EcoT14I	CCWWGG	2 cut(s)	79, 2063
EcoT22I	ATGCAT	1 cut(s)	2169
EcoT38I	GRGCYC	1 cut(s)	1246
ErhI	CCWWGG	2 cut(s)	79, 2063
FaeI	CATG	8 cut(s)	489, 605, 924, 1008, 1316, 1615, 1634, 1813
FalI	AAGNNNNNCTT	2 cut(s)	224, 256
FaqI	GGGAC	3 cut(s)	296, 876, 1906
FatI	CATG	8 cut(s)	485, 601, 920, 1004, 1312, 1611, 1630, 1809
FauNDI	CATATG	1 cut(s)	109
FbaI	TGATCA	2 cut(s)	1633, 1861
FblI	GTMKAC	1 cut(s)	1413
Fnu4HI	GCNGC	9 cut(s)	50, 943, 1180, 1224, 1356, 1672, 1719, 1757, 2114
FokI	GGATG	6 cut(s)	76, 882, 1060, 1711, 1727, 1814
FriOI	GRGCYC	1 cut(s)	1246
Fsp4HI	GCNGC	9 cut(s)	50, 943, 1180, 1224, 1356, 1672, 1719, 1757, 2114
FspBI	CTAG	8 cut(s)	80, 309, 393, 876, 1467, 1562, 2147, 2199
GlaI	GCGC	2 cut(s)	376, 1504
GluI	GCNGC	9 cut(s)	50, 943, 1180, 1224, 1356, 1672, 1719, 1757, 2114
GsaI	CCCAGC	3 cut(s)	60, 283, 760
GsuI	CTGGAG	2 cut(s)	430, 612
HaeIII	GGCC	9 cut(s)	78, 683, 696, 1448, 1852, 1886, 2062, 2132, 2197
HapII	CCGG	9 cut(s)	181, 253, 258, 657, 914, 1198, 1395, 1853, 2117
HgaI	GACGC	1 cut(s)	160
HhaI	GCGC	2 cut(s)	377, 1505
Hin1II	CATG	8 cut(s)	489, 605, 924, 1008, 1316, 1615, 1634, 1813
Hin6I	GCGC	2 cut(s)	375, 1503
HinP1I	GCGC	2 cut(s)	375, 1503
HincII	GTYRAC	3 cut(s)	120, 342, 388
HindII	GTYRAC	3 cut(s)	120, 342, 388
HindIII	AAGCTT	3 cut(s)	580, 1091, 2089
HinfI	GANTC	8 cut(s)	513, 547, 596, 653, 794, 970, 1202, 1511
HpaII	CCGG	9 cut(s)	181, 253, 258, 657, 914, 1198, 1395, 1853, 2117
HphI	GGTGA	4 cut(s)	371, 426, 1532, 1567
Hpy188I	TCNGA	9 cut(s)	883, 967, 1207, 1432, 1516, 1828, 1861, 1967, 2175
Hpy188III	TCNNGA	9 cut(s)	457, 471, 562, 1439, 1453, 1631, 1772, 1865, 1913
Hpy99I	CGWCG	1 cut(s)	335
HpyAV	CCTTC	6 cut(s)	46, 247, 281, 2018, 2297, 2301
HpyCH4IV	ACGT	5 cut(s)	116, 313, 333, 1263, 1932
HpyF10VI	GCNNNNNNNGC	9 cut(s)	276, 297, 707, 1005, 1534, 1715, 2113, 2119, 2159
HpyF3I	CTNAG	4 cut(s)	543, 1206, 1362, 2174
HpySE526I	ACGT	5 cut(s)	116, 313, 333, 1263, 1932
Hsp92II	CATG	8 cut(s)	489, 605, 924, 1008, 1316, 1615, 1634, 1813
HspAI	GCGC	2 cut(s)	375, 1503
KroI	GCCGGC	1 cut(s)	1852
KroNI	GCCGGC	1 cut(s)	1854
Ksp22I	TGATCA	2 cut(s)	1633, 1861
LmnI	GCTCC	6 cut(s)	539, 707, 1279, 1398, 1981, 2119
Lsp1109I	GCAGC	8 cut(s)	36, 954, 1191, 1210, 1342, 1683, 1705, 2125
LweI	GCATC	5 cut(s)	98, 352, 1705, 1792, 1865
MaeI	CTAG	8 cut(s)	80, 309, 393, 876, 1467, 1562, 2147, 2199
MaeII	ACGT	5 cut(s)	116, 313, 333, 1263, 1932
MaeIII	GTNAC	5 cut(s)	326, 463, 761, 892, 2012
MfeI	CAATTG	3 cut(s)	662, 2037, 2235
MflI	RGATCY	2 cut(s)	1254, 2080
MhlI	GDGCHC	1 cut(s)	1246
MlsI	TGGCCA	1 cut(s)	1448
MluNI	TGGCCA	1 cut(s)	1448
MlyI	GAGTC	5 cut(s)	522, 605, 803, 964, 1211
MmeI	TCCRAC	6 cut(s)	220, 757, 1067, 1096, 1959, 2076
Mox20I	TGGCCA	1 cut(s)	1448
Mph1103I	ATGCAT	1 cut(s)	2169
MroNI	GCCGGC	1 cut(s)	1852
MroXI	GAANNNNTTC	3 cut(s)	236, 784, 2139
MscI	TGGCCA	1 cut(s)	1448
MseI	TTAA	3 cut(s)	20, 572, 584
MslI	CAYNNNNRTG	3 cut(s)	606, 768, 1746
Msp20I	TGGCCA	1 cut(s)	1448
MspA1I	CMGCKG	1 cut(s)	945
MspI	CCGG	9 cut(s)	181, 253, 258, 657, 914, 1198, 1395, 1853, 2117
MspR9I	CCNGG	7 cut(s)	438, 658, 867, 915, 1199, 1396, 1490
MunI	CAATTG	3 cut(s)	662, 2037, 2235
MvaI	CCWGG	3 cut(s)	438, 867, 1490
MvnI	CGCG	2 cut(s)	1440, 1505
MwoI	GCNNNNNNNGC	9 cut(s)	276, 297, 707, 1005, 1534, 1715, 2113, 2119, 2159
NaeI	GCCGGC	1 cut(s)	1854
NciI	CCSGG	4 cut(s)	658, 915, 1199, 1396
NdeI	CATATG	1 cut(s)	109
NgoMIV	GCCGGC	1 cut(s)	1852
NlaIII	CATG	8 cut(s)	489, 605, 924, 1008, 1316, 1615, 1634, 1813
NlaIV	GGNNCC	7 cut(s)	682, 864, 918, 1393, 1851, 2121, 2256
NmeAIII	GCCGAG	1 cut(s)	1513
NmuCI	GTSAC	4 cut(s)	326, 463, 761, 2012
NruI	TCGCGA	1 cut(s)	1440
NsiI	ATGCAT	1 cut(s)	2169
NspI	RCATGY	2 cut(s)	489, 1316
PaeI	GCATGC	1 cut(s)	1316
PagI	TCATGA	1 cut(s)	1630
PceI	AGGCCT	2 cut(s)	696, 2197
PciI	ACATGT	1 cut(s)	485
PcsI	WCGNNNNNNNCGW	1 cut(s)	794
PdiI	GCCGGC	1 cut(s)	1854
PdmI	GAANNNNTTC	3 cut(s)	236, 784, 2139
PfeI	GAWTC	3 cut(s)	547, 653, 1511
PfoI	TCCNGGA	1 cut(s)	1488
PkrI	GCNGC	9 cut(s)	51, 944, 1181, 1225, 1357, 1673, 1720, 1758, 2115
PleI	GAGTC	5 cut(s)	521, 604, 802, 964, 1210
PpsI	GAGTC	5 cut(s)	521, 604, 802, 964, 1210
PscI	ACATGT	1 cut(s)	485
Psp1406I	AACGTT	2 cut(s)	1263, 1932
Psp6I	CCWGG	3 cut(s)	436, 865, 1488
PspFI	CCCAGC	3 cut(s)	56, 279, 756
PspGI	CCWGG	3 cut(s)	436, 865, 1488
PspN4I	GGNNCC	7 cut(s)	682, 864, 918, 1393, 1851, 2121, 2256
PspPI	GGNCC	7 cut(s)	255, 681, 863, 917, 1850, 2033, 2060
PstNI	CAGNNNCTG	1 cut(s)	408
PsuI	RGATCY	2 cut(s)	1254, 2080
PvuII	CAGCTG	1 cut(s)	945
RruI	TCGCGA	1 cut(s)	1440
RsaI	GTAC	8 cut(s)	672, 927, 956, 1471, 1610, 1904, 1919, 1926
RsaNI	GTAC	8 cut(s)	671, 926, 955, 1470, 1609, 1903, 1918, 1925
RseI	CAYNNNNRTG	3 cut(s)	606, 768, 1746
Rsr2I	CGGWCCG	1 cut(s)	255
RsrII	CGGWCCG	1 cut(s)	255
SaqAI	TTAA	3 cut(s)	20, 572, 584
SatI	GCNGC	9 cut(s)	50, 943, 1180, 1224, 1356, 1672, 1719, 1757, 2114
Sau96I	GGNCC	7 cut(s)	255, 681, 863, 917, 1850, 2033, 2060
ScaI	AGTACT	1 cut(s)	1919
SchI	GAGTC	5 cut(s)	522, 605, 803, 964, 1211
ScrFI	CCNGG	7 cut(s)	438, 658, 867, 915, 1199, 1396, 1490
SduI	GDGCHC	1 cut(s)	1246
SfaNI	GCATC	5 cut(s)	98, 352, 1705, 1792, 1865
SinI	GGWCC	4 cut(s)	255, 863, 917, 2033
SmiMI	CAYNNNNRTG	3 cut(s)	606, 768, 1746
SmlI	CTYRAG	2 cut(s)	366, 613
SmoI	CTYRAG	2 cut(s)	366, 613
SpeI	ACTAGT	1 cut(s)	1466
SphI	GCATGC	1 cut(s)	1316
SseBI	AGGCCT	2 cut(s)	696, 2197
SsiI	CCGC	2 cut(s)	1757, 2277
SspMI	CTAG	8 cut(s)	80, 309, 393, 876, 1467, 1562, 2147, 2199
StuI	AGGCCT	2 cut(s)	696, 2197
StyD4I	CCNGG	7 cut(s)	436, 656, 865, 913, 1197, 1394, 1488
StyI	CCWWGG	2 cut(s)	79, 2063
TaiI	ACGT	5 cut(s)	119, 316, 336, 1266, 1935
TaqI	TCGA	4 cut(s)	561, 797, 905, 1771
TaqII	GACCGA	1 cut(s)	1028
TatI	WGTACW	5 cut(s)	925, 1469, 1608, 1917, 1924
TauI	GCSGC	1 cut(s)	1759
TfiI	GAWTC	3 cut(s)	547, 653, 1511
Tru1I	TTAA	3 cut(s)	20, 572, 584
Tru9I	TTAA	3 cut(s)	20, 572, 584
TseFI	GTSAC	4 cut(s)	326, 463, 761, 2012
TseI	GCWGC	8 cut(s)	49, 942, 1179, 1223, 1355, 1671, 1718, 2113
Tsp45I	GTSAC	4 cut(s)	326, 463, 761, 2012
TspDTI	ATGAA	7 cut(s)	28, 148, 515, 1059, 1290, 1619, 1757
VpaK11BI	GGWCC	4 cut(s)	255, 863, 917, 2033
XapI	RAATTY	4 cut(s)	712, 961, 1331, 1484
XceI	RCATGY	2 cut(s)	489, 1316
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1385
XmaJI	CCTAGG	1 cut(s)	79
XmiI	GTMKAC	1 cut(s)	1413
XmnI	GAANNNNTTC	3 cut(s)	236, 784, 2139
XspI	CTAG	8 cut(s)	80, 309, 393, 876, 1467, 1562, 2147, 2199
ZrmI	AGTACT	1 cut(s)	1919
Zsp2I	ATGCAT	1 cut(s)	2169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.