Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0003366

Physical Location & Seq

Genomic Coordinates Reverse (-)

16460319 .. 16462736

Gene Structure 2418 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g17070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGACGAAACTACCCTTCTCCTCCCTCTCCACCACCACCTTCATCATCACCGCCGCCATCCTCTTCGCCGTTATCCCACCACCAGCTCATGCCCTCGGCTCCGGCTCAACCCTCGCCGTAATATCCACTACCGGCACCGTCTGTGGTGTCGTTTCGGGCCAACCGCGGCTGCGCATCGTCTGCTACCGCCGCGGCCAAACCGTCGCGGTCGAGCCCAACATTTCATTCTTAGCCATCTCCGGCGGCCGAACCTCCCTCTGCGGCATCCGCTCTGGCGGATACAGCCTCCTCTGCTGGGACAACATCACCTCCCAAAACTCCACCTTCAAATCCAAACGCCTTTACTTCAACAGAACCGTTCCGTTAGAAAATCTCGCAGTCGGCGACGACCATATCTGCGCCAGAACCGTCGACACAGACACCGTAGAATGCTGGCGGCCCAACGACGTGCCCTACTTGCCATCTGGGTCGGACAAATTCTCCTCGATTTCATCTGGATCGGACTTCTCTTGCGGAATTCTGAAACCCAGCCTCGAGGTTCGATGCTGGGGCACAGCAACTGCAATGGCGGAAGAAATACAACACGGGTTCGGAAACATGTCAATGGCGACCATCGCAGCTGGAGGCTCGCATGTGTGTGGGATCAACACCACCGGGCTTCTGGTCTGCAGGGGAAGCAATGGCAGCGGTCAGTTGAACGCCCCTTCTGGCTCGCCTTTTGAGTACTCCGCTCTGTCACTCGGAGCAAATCACAGCTGCGCAATCAGAACTCATAATCAAACAGTCGTCTGCTGGGGAGGAATGGGCCAGTTTTCAATTAACAAAACAAGTGGAGTGTCTTTTCAAATTTTAGCTTCTGGGTCGGATTTCATATGTGGATTAACCACCGGGAATTTTTCGATTGTTTGTTGGGGTCCGGGCTGGCCCATTCGCTCCGGTTCAGAATCAGGCGTGGAGCTCGAATTGCCCGAAATTCTCCCCGGTTCTTGTGTCAATTCCTCCTGCAGTGAATGTGGAATTTACCCTCAGTCTCAAAGACTCTGTTTTGGTACTGGTAACATTTGCAACCCCTGTTTTCCTCCAATTTCAGTTCCCCCACCAGCATTACCACCGCCGCCATCAACACCGCCACCGACGTCAACGCGGCGGTCTGCGACGTTGACTACTGGTTTATTGGTGTTTGCCATTGTGGGTTCTGTCGGAGGATTTGCGGGTATTTGTACAGTTATTTATTGTTTGTGGATTGGCGGTTGTTTTGGGAACAAGAAGATTCATAATTCAGTTCAGCCGACAATTACTAGAGCTGCAAGCTCAAACAATGGCAATGGGAGTACGTCAAACAATAGTCCTCCGTCGAGATCGTCCACCATCCGCCGCCAGGGTTCGAGGATCATGCGGCGGCAGAGGAGCGGAACATCCTCGAAGCACCCTGACAGGGCGGAGGAGTTTTCTCTGTCCGAGCTCGCGGCCGCCACGAATGACTTCTCAATGGAGAACAAGATTGGCTCCGGAAGCTTCGGAGTTGTATATAGGGGCAAACTGCAAGACGGCCGGGAAGTTGCGATCAAACGAGGCGAAACCGCCTCGAAGATGAAGAAGTTTCAGGAGAAGGAGAGCGCATTCGATTCGGAAGTTGCCTTCTTGTCGAGAGTTCATCACAAGCACCTCGTGAGGCTTGTCGGATATTGCGAAGAGAGGGATGAGAGGCTTTTGGTATATGAGTACATGAAAAACGGTGCGCTTTTCAATCACTTGCATGACAAGAACAATGTGGAGAAGAGTAGCAGTTTGTTGAATTCGTGGAGAGTGAGGATCAAAGTTGCATTGGATGCTTCAAGAGGCATTGAGTATCTGCACAATTACGCCGTGCCGCCTATCATTCATCGAGATATAAAATCATCGAATATCTTGCTCGACGCGAATTGGACCGGGAGGGTGTCTGATTTCGGGTTGTCGTTGATGGGGCCGGACGCAGAGGCGGATTTTAGGCCAACGAAGGCGGCAGGGACGGTTGGCTACATTGATCCGGAGTACTATGGACTCAACTTGTTGACAGCAAAGAGCGATGTCTATGGACTTGGTGTGGTGCTATTGGAGCTTCTGACGGGGAAGAGGGCCATATTTAAGGATGCGGAGAACGAGGGGACGCCTATAAGCGTGGTGGATTTCGCAGTGCCCGCGATTATGAGCGGGGAGTTGGTGAGGGTTTTGGACAGGAGGGTTGGGCCACCGGAGGTGAATGAATCGGAGGCGATTGAGCTGATGGCGTATACCGCAATGCATTGCGTGAATTTGGAAGGGAAAGATAGGCCGACGATGGCGGACATTGTGGCGAATTTGGAGAGGGCGTTTGCTCTCTGCGATGAAAGCCCCGGCAACATCTCTAGTGGTGGAAACTCCATTGTGTCTGCAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr RCC1_2

Protein Analysis

806

Amino Acids

86.07

Weight (kDa)

7.71

Isoelectric Point (pI)

45.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	496 - 778	5.4e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	496 - 780	7.8e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55950
fragaria_vesca	FvH4_3g38710 FvH4_7g24490
malus_domestica	MD00G1166500.v1.1 MD01G1153100.v1.1 MD03G1068800.v1.1 MD07G1222100.v1.1 MD11G1073300.v1.1
prunus_persica	Prupe.2G250300_v2.0.a1 Prupe.6G056600_v2.0.a1
pyrus_communis	pycom01g17070 pycom03g05510
rosa_chinensis	RchiOBHm_Chr1g0370251 RchiOBHm_Chr5g0065501 RchiOBHm_Chr5g0069861
rosa_laevigata	RLG00000027065 RLG00000036103
rosa_multiflora	Rmu_co8321277.1_g000001 Rmu_sc0000738.1_g000002 Rmu_sc0026893.1_g000001 Rmu_sc0037931.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011190 Rroxscaffold_4G00286710 Rroxscaffold_4G00287300
rosa_rugosa	Rorug01G0355200 Rorug05G0400300
rosa_samantha	Rh1AG363800 Rh1BG326900 Rh1CG340200 Rh1DG357300 Rh5AG457400 Rh5BG476200 Rh5CG500000 Rh5DG488000
rosa_wichuraiana	Rw1G032010 Rw5G042700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1139
AbsI	CCTCGAGG	1 cut(s)	533
Acc16I	TGCGCA	2 cut(s)	173, 760
AccB1I	GGYRCC	1 cut(s)	134
AccBSI	CCGCTC	4 cut(s)	270, 731, 1410, 2190
AccI	GTMKAC	2 cut(s)	411, 2270
AccII	CGCG	7 cut(s)	166, 192, 206, 1144, 1466, 1919, 2180
AccIII	TCCGGA	2 cut(s)	1508, 2026
AclWI	GGATC	5 cut(s)	505, 650, 1397, 1820, 2018
AcoI	YGGCCR	4 cut(s)	193, 244, 1467, 1549
AcsI	RAATTY	9 cut(s)	476, 516, 846, 892, 972, 1017, 1795, 2290, 2335
AcyI	GRCGYC	2 cut(s)	1136, 2147
AdeI	CACNNNGTG	1 cut(s)	1669
AfaI	GTAC	6 cut(s)	725, 1051, 1222, 1333, 1724, 2033
AfiI	CCNNNNNNNGG	3 cut(s)	295, 1378, 1435
AflIII	ACRYGT	1 cut(s)	597
AgsI	TTSAA	8 cut(s)	328, 349, 697, 816, 845, 1747, 1795, 1836
AjiI	CACGTC	1 cut(s)	448
AjnI	CCWGG	1 cut(s)	1377
AjuI	GAANNNNNNNTTGG	2 cut(s)	209, 241
Alw21I	GWGCWC	2 cut(s)	960, 1464
Alw26I	GTCTC	1 cut(s)	1035
AlwI	GGATC	5 cut(s)	505, 650, 1397, 1820, 2018
AlwNI	CAGNNNCTG	1 cut(s)	560
Ama87I	CYCGRG	1 cut(s)	533
Aor13HI	TCCGGA	2 cut(s)	1508, 2026
ApeKI	GCWGC	5 cut(s)	169, 617, 684, 756, 1304
ApoI	RAATTY	9 cut(s)	476, 516, 846, 892, 972, 1017, 1795, 2290, 2335
Asp700I	GAANNNNTTC	1 cut(s)	1481
AspLEI	GCGC	5 cut(s)	174, 401, 761, 1619, 1741
AspS9I	GGNCC	9 cut(s)	157, 438, 805, 914, 924, 1926, 1964, 2115, 2225
AsuC2I	CCSGG	7 cut(s)	655, 889, 918, 981, 1553, 1930, 2373
AsuHPI	GGTGA	4 cut(s)	40, 298, 2212, 2248
AvaI	CYCGRG	1 cut(s)	533
AvaII	GGWCC	2 cut(s)	914, 1926
BaeGI	GKGCMC	3 cut(s)	453, 554, 2178
BanI	GGYRCC	1 cut(s)	134
BanII	GRGCYC	3 cut(s)	216, 960, 1464
BauI	CACGAG	1 cut(s)	1667
Bbv12I	GWGCWC	2 cut(s)	960, 1464
BbvI	GCAGC	5 cut(s)	156, 629, 696, 743, 1291
BccI	CCATC	9 cut(s)	65, 242, 469, 620, 1126, 1376, 1954, 2257, 2311
BceAI	ACGGC	4 cut(s)	53, 101, 1564, 1850
BcgI	CGANNNNNNTGC	6 cut(s)	1375, 1409, 1608, 1642, 1891, 1925
BciT130I	CCWGG	1 cut(s)	1379
BciVI	GTATCC	1 cut(s)	272
BcnI	CCSGG	7 cut(s)	655, 889, 918, 981, 1553, 1930, 2373
BcoDI	GTCTC	1 cut(s)	1035
BfaI	CTAG	2 cut(s)	1299, 2385
BfmI	CTRYAG	3 cut(s)	667, 1003, 2409
BfuI	GTATCC	1 cut(s)	272
BmcAI	AGTACT	2 cut(s)	725, 2033
Bme1390I	CCNGG	8 cut(s)	655, 889, 918, 981, 1379, 1553, 1930, 2373
Bme18I	GGWCC	2 cut(s)	914, 1926
BmeT110I	CYCGRG	1 cut(s)	533
BmgBI	CACGTC	1 cut(s)	448
BmgT120I	GGNCC	9 cut(s)	157, 438, 805, 914, 924, 1926, 1964, 2115, 2225
BmiI	GGNNCC	5 cut(s)	100, 136, 915, 1507, 1965
BmrFI	CCNGG	8 cut(s)	655, 889, 918, 981, 1379, 1553, 1930, 2373
BmsI	GCATC	5 cut(s)	183, 273, 533, 1819, 2119
BpmI	CTGGAG	1 cut(s)	642
BpuMI	CCSGG	7 cut(s)	655, 889, 918, 981, 1553, 1930, 2373
BsaHI	GRCGYC	2 cut(s)	1136, 2147
BsaJI	CCNNGG	6 cut(s)	94, 164, 190, 979, 1378, 2371
BsaWI	WCCGGW	4 cut(s)	935, 1508, 2026, 2230
BsaXI	ACNNNNNCTCC	6 cut(s)	293, 323, 464, 494, 2333, 2363
Bsc4I	CCNNNNNNNGG	3 cut(s)	295, 1378, 1435
Bse118I	RCCGGY	1 cut(s)	131
Bse1I	ACTGG	3 cut(s)	808, 1057, 1171
Bse3DI	GCAATG	5 cut(s)	570, 685, 1330, 2281, 2283
BseAI	TCCGGA	2 cut(s)	1508, 2026
BseBI	CCWGG	1 cut(s)	1379
BseDI	CCNNGG	6 cut(s)	94, 164, 190, 979, 1378, 2371
BseGI	GGATG	7 cut(s)	57, 264, 1368, 1415, 1705, 1834, 2134
BseLI	CCNNNNNNNGG	3 cut(s)	295, 1378, 1435
BseMI	GCAATG	5 cut(s)	570, 685, 1330, 2281, 2283
BseMII	CTCAG	1 cut(s)	1040
BseNI	ACTGG	3 cut(s)	808, 1057, 1171
BseRI	GAGGAG	5 cut(s)	10, 278, 472, 1420, 1457
BseSI	GKGCMC	3 cut(s)	453, 554, 2178
BseX3I	CGGCCG	3 cut(s)	244, 1467, 1549
BseXI	GCAGC	5 cut(s)	156, 629, 696, 743, 1291
BseYI	CCCAGC	4 cut(s)	294, 527, 546, 792
BsgI	GTGCAG	1 cut(s)	1838
Bsh1236I	CGCG	7 cut(s)	166, 192, 206, 1144, 1466, 1919, 2180
Bsh1285I	CGRYCG	4 cut(s)	210, 247, 1470, 1552
BshNI	GGYRCC	1 cut(s)	134
BsiEI	CGRYCG	4 cut(s)	210, 247, 1470, 1552
BsiHKAI	GWGCWC	2 cut(s)	960, 1464
BsiHKCI	CYCGRG	1 cut(s)	533
BslFI	GGGAC	3 cut(s)	311, 2020, 2158
BslI	CCNNNNNNNGG	3 cut(s)	295, 1378, 1435
BsmAI	GTCTC	1 cut(s)	1035
BsmFI	GGGAC	3 cut(s)	311, 2020, 2158
BsmI	GAATGC	2 cut(s)	434, 1619
BsoBI	CYCGRG	1 cut(s)	533
Bsp1286I	GDGCHC	6 cut(s)	216, 453, 554, 960, 1464, 2178
Bsp13I	TCCGGA	2 cut(s)	1508, 2026
Bsp1407I	TGTACA	1 cut(s)	1220
Bsp143I	GATC	7 cut(s)	497, 642, 1358, 1389, 1563, 1812, 2023
BspCNI	CTCAG	1 cut(s)	1039
BspEI	TCCGGA	2 cut(s)	1508, 2026
BspFNI	CGCG	7 cut(s)	166, 192, 206, 1144, 1466, 1919, 2180
BspLI	GGNNCC	5 cut(s)	100, 136, 915, 1507, 1965
BspMAI	CTGCAG	3 cut(s)	671, 1007, 2413
BspPI	GGATC	5 cut(s)	505, 650, 1397, 1820, 2018
BspT107I	GGYRCC	1 cut(s)	134
BsrBI	CCGCTC	4 cut(s)	270, 731, 1410, 2190
BsrDI	GCAATG	5 cut(s)	570, 685, 1330, 2281, 2283
BsrFI	RCCGGY	1 cut(s)	131
BsrGI	TGTACA	1 cut(s)	1220
BsrI	ACTGG	3 cut(s)	808, 1057, 1171
BssAI	RCCGGY	1 cut(s)	131
BssECI	CCNNGG	6 cut(s)	94, 164, 190, 979, 1378, 2371
BssMI	GATC	7 cut(s)	497, 642, 1358, 1389, 1563, 1812, 2023
BssNAI	GTATAC	1 cut(s)	2271
BssNI	GRCGYC	2 cut(s)	1136, 2147
BssSI	CACGAG	1 cut(s)	1667
Bst1107I	GTATAC	1 cut(s)	2271
Bst2BI	CACGAG	1 cut(s)	1667
Bst2UI	CCWGG	1 cut(s)	1379
Bst4CI	ACNGT	9 cut(s)	139, 202, 358, 409, 424, 784, 1225, 1736, 2011
Bst6I	CTCTTC	4 cut(s)	68, 1686, 1772, 2105
BstACI	GRCGYC	2 cut(s)	1136, 2147
BstAPI	GCANNNNNTGC	2 cut(s)	180, 1829
BstAUI	TGTACA	1 cut(s)	1220
BstC8I	GCNNGC	7 cut(s)	434, 629, 713, 923, 1309, 1464, 2178
BstDEI	CTNAG	2 cut(s)	229, 1026
BstDSI	CCRYGG	2 cut(s)	164, 190
BstF5I	GGATG	7 cut(s)	57, 264, 1368, 1415, 1705, 1834, 2134
BstFNI	CGCG	7 cut(s)	166, 192, 206, 1144, 1466, 1919, 2180
BstHHI	GCGC	5 cut(s)	174, 401, 761, 1619, 1741
BstKTI	GATC	7 cut(s)	500, 645, 1361, 1392, 1566, 1815, 2026
BstMAI	GTCTC	1 cut(s)	1035
BstMBI	GATC	7 cut(s)	497, 642, 1358, 1389, 1563, 1812, 2023
BstMCI	CGRYCG	4 cut(s)	210, 247, 1470, 1552
BstNI	CCWGG	1 cut(s)	1379
BstNSI	RCATGY	2 cut(s)	601, 635
BstSCI	CCNGG	8 cut(s)	653, 887, 916, 979, 1377, 1551, 1928, 2371
BstSFI	CTRYAG	3 cut(s)	667, 1003, 2409
BstSLI	GKGCMC	3 cut(s)	453, 554, 2178
BstUI	CGCG	7 cut(s)	166, 192, 206, 1144, 1466, 1919, 2180
BstV1I	GCAGC	5 cut(s)	156, 629, 696, 743, 1291
BstZ17I	GTATAC	1 cut(s)	2271
BstZI	CGGCCG	3 cut(s)	244, 1467, 1549
BsuI	GTATCC	1 cut(s)	272
BtgI	CCRYGG	2 cut(s)	164, 190
BtgZI	GCGATG	3 cut(s)	598, 2079, 2376
BtrI	CACGTC	1 cut(s)	448
BtsCI	GGATG	7 cut(s)	57, 264, 1368, 1415, 1705, 1834, 2134
BtsI	GCAGTG	2 cut(s)	1012, 2178
BtsIMutI	CAGTG	2 cut(s)	1012, 2178
Cac8I	GCNNGC	7 cut(s)	434, 629, 713, 923, 1309, 1464, 2178
CaiI	CAGNNNCTG	1 cut(s)	560
CciNI	GCGGCCGC	1 cut(s)	1467
CfoI	GCGC	5 cut(s)	174, 401, 761, 1619, 1741
Cfr10I	RCCGGY	1 cut(s)	131
Cfr13I	GGNCC	9 cut(s)	157, 438, 805, 914, 924, 1926, 1964, 2115, 2225
Cfr42I	CCGCGG	2 cut(s)	167, 193
CseI	GACGC	3 cut(s)	1925, 1979, 2155
Csp6I	GTAC	6 cut(s)	724, 1050, 1221, 1332, 1723, 2032
CspCI	CAANNNNNGTGG	2 cut(s)	811, 846
CviAII	CATG	6 cut(s)	89, 598, 632, 1393, 1726, 1757
CviQI	GTAC	6 cut(s)	724, 1050, 1221, 1332, 1723, 2032
DdeI	CTNAG	2 cut(s)	229, 1026
DpnI	GATC	7 cut(s)	499, 644, 1360, 1391, 1565, 1814, 2025
DpnII	GATC	7 cut(s)	497, 642, 1358, 1389, 1563, 1812, 2023
DraIII	CACNNNGTG	1 cut(s)	1669
EaeI	YGGCCR	4 cut(s)	193, 244, 1467, 1549
EagI	CGGCCG	3 cut(s)	244, 1467, 1549
Eam1104I	CTCTTC	4 cut(s)	68, 1686, 1772, 2105
EarI	CTCTTC	4 cut(s)	68, 1686, 1772, 2105
EciI	GGCGGA	6 cut(s)	291, 584, 1361, 1454, 1994, 2336
Ecl136II	GAGCTC	2 cut(s)	958, 1462
EclXI	CGGCCG	3 cut(s)	244, 1467, 1549
Eco24I	GRGCYC	3 cut(s)	216, 960, 1464
Eco47I	GGWCC	2 cut(s)	914, 1926
Eco52I	CGGCCG	3 cut(s)	244, 1467, 1549
Eco53kI	GAGCTC	2 cut(s)	958, 1462
Eco88I	CYCGRG	1 cut(s)	533
EcoICRI	GAGCTC	2 cut(s)	958, 1462
EcoRI	GAATTC	2 cut(s)	516, 1795
EcoRII	CCWGG	1 cut(s)	1377
EcoT22I	ATGCAT	1 cut(s)	2283
EcoT38I	GRGCYC	3 cut(s)	216, 960, 1464
FaeI	CATG	6 cut(s)	92, 601, 635, 1396, 1729, 1760
FaqI	GGGAC	3 cut(s)	311, 2020, 2158
FatI	CATG	6 cut(s)	88, 597, 631, 1392, 1725, 1756
FauI	CCCGC	3 cut(s)	1204, 2183, 2185
FauNDI	CATATG	1 cut(s)	872
FblI	GTMKAC	2 cut(s)	411, 2270
FokI	GGATG	7 cut(s)	44, 251, 1355, 1402, 1712, 1841, 2141
FriOI	GRGCYC	3 cut(s)	216, 960, 1464
FspBI	CTAG	2 cut(s)	1299, 2385
FspI	TGCGCA	2 cut(s)	173, 760
GlaI	GCGC	5 cut(s)	173, 400, 760, 1618, 1740
GsaI	CCCAGC	4 cut(s)	298, 531, 550, 796
GsuI	CTGGAG	1 cut(s)	642
HgaI	GACGC	3 cut(s)	1925, 1979, 2155
HhaI	GCGC	5 cut(s)	174, 401, 761, 1619, 1741
Hin1I	GRCGYC	2 cut(s)	1136, 2147
Hin1II	CATG	6 cut(s)	92, 601, 635, 1396, 1729, 1760
Hin6I	GCGC	5 cut(s)	172, 399, 759, 1617, 1739
HinP1I	GCGC	5 cut(s)	172, 399, 759, 1617, 1739
HincII	GTYRAC	4 cut(s)	412, 1140, 1161, 2052
HindII	GTYRAC	4 cut(s)	412, 1140, 1161, 2052
HindIII	AAGCTT	1 cut(s)	1513
HinfI	GANTC	6 cut(s)	944, 1038, 1270, 1625, 2040, 2243
HphI	GGTGA	4 cut(s)	40, 298, 2212, 2248
Hpy166II	GTNNAC	6 cut(s)	412, 1140, 1161, 1365, 2052, 2271
Hpy188III	TCNNGA	9 cut(s)	495, 1356, 1509, 1604, 1647, 1669, 1836, 1886, 2027
Hpy8I	GTNNAC	6 cut(s)	412, 1140, 1161, 1365, 2052, 2271
Hpy99I	CGWCG	9 cut(s)	206, 389, 413, 449, 1138, 1159, 1357, 1919, 2317
HpyAV	CCTTC	8 cut(s)	25, 49, 334, 714, 1603, 1648, 1990, 2291
HpyCH4III	ACNGT	9 cut(s)	139, 202, 358, 409, 424, 784, 1225, 1736, 2011
HpyCH4IV	ACGT	4 cut(s)	447, 1136, 1157, 1334
HpyF3I	CTNAG	2 cut(s)	229, 1026
HpySE526I	ACGT	4 cut(s)	447, 1136, 1157, 1334
Hsp92I	GRCGYC	2 cut(s)	1136, 2147
Hsp92II	CATG	6 cut(s)	92, 601, 635, 1396, 1729, 1760
HspAI	GCGC	5 cut(s)	172, 399, 759, 1617, 1739
Kpn2I	TCCGGA	2 cut(s)	1508, 2026
KspI	CCGCGG	2 cut(s)	167, 193
Kzo9I	GATC	7 cut(s)	497, 642, 1358, 1389, 1563, 1812, 2023
LmnI	GCTCC	7 cut(s)	104, 743, 938, 955, 1407, 1511, 2095
Lsp1109I	GCAGC	5 cut(s)	156, 629, 696, 743, 1291
LweI	GCATC	5 cut(s)	183, 273, 533, 1819, 2119
MaeI	CTAG	2 cut(s)	1299, 2385
MaeII	ACGT	4 cut(s)	447, 1136, 1157, 1334
MaeIII	GTNAC	2 cut(s)	735, 1055
MalI	GATC	7 cut(s)	499, 644, 1360, 1391, 1565, 1814, 2025
MbiI	CCGCTC	4 cut(s)	270, 731, 1410, 2190
MboI	GATC	7 cut(s)	497, 642, 1358, 1389, 1563, 1812, 2023
MboII	GAAGA	8 cut(s)	55, 584, 1279, 1600, 1606, 1703, 1789, 2122
MhlI	GDGCHC	6 cut(s)	216, 453, 554, 960, 1464, 2178
MlyI	GAGTC	2 cut(s)	1032, 2034
MmeI	TCCRAC	4 cut(s)	450, 843, 1180, 1660
Mph1103I	ATGCAT	1 cut(s)	2283
MroI	TCCGGA	2 cut(s)	1508, 2026
MroXI	GAANNNNTTC	1 cut(s)	1481
MseI	TTAA	3 cut(s)	819, 881, 2124
MslI	CAYNNNNRTG	3 cut(s)	602, 636, 1755
MspA1I	CMGCKG	5 cut(s)	166, 192, 620, 687, 756
MspR9I	CCNGG	8 cut(s)	655, 889, 918, 981, 1379, 1553, 1930, 2373
Mva1269I	GAATGC	2 cut(s)	434, 1619
MvaI	CCWGG	1 cut(s)	1379
MvnI	CGCG	7 cut(s)	166, 192, 206, 1144, 1466, 1919, 2180
NciI	CCSGG	7 cut(s)	655, 889, 918, 981, 1553, 1930, 2373
NdeI	CATATG	1 cut(s)	872
NdeII	GATC	7 cut(s)	497, 642, 1358, 1389, 1563, 1812, 2023
NlaIII	CATG	6 cut(s)	92, 601, 635, 1396, 1729, 1760
NlaIV	GGNNCC	5 cut(s)	100, 136, 915, 1507, 1965
NmeAIII	GCCGAG	1 cut(s)	75
NmuCI	GTSAC	1 cut(s)	735
NotI	GCGGCCGC	1 cut(s)	1467
NsbI	TGCGCA	2 cut(s)	173, 760
NsiI	ATGCAT	1 cut(s)	2283
NspI	RCATGY	2 cut(s)	601, 635
PaeR7I	CTCGAG	1 cut(s)	533
PciI	ACATGT	1 cut(s)	597
PcsI	WCGNNNNNNNCGW	3 cut(s)	381, 966, 1686
PctI	GAATGC	2 cut(s)	434, 1619
PdmI	GAANNNNTTC	1 cut(s)	1481
PfeI	GAWTC	4 cut(s)	944, 1270, 1625, 2243
PleI	GAGTC	2 cut(s)	1032, 2034
PpsI	GAGTC	2 cut(s)	1032, 2034
PscI	ACATGT	1 cut(s)	597
Psp124BI	GAGCTC	2 cut(s)	960, 1464
Psp6I	CCWGG	1 cut(s)	1377
PspFI	CCCAGC	4 cut(s)	294, 527, 546, 792
PspGI	CCWGG	1 cut(s)	1377
PspN4I	GGNNCC	5 cut(s)	100, 136, 915, 1507, 1965
PspPI	GGNCC	9 cut(s)	157, 438, 805, 914, 924, 1926, 1964, 2115, 2225
PspXI	VCTCGAGB	1 cut(s)	533
PstI	CTGCAG	3 cut(s)	671, 1007, 2413
PstNI	CAGNNNCTG	1 cut(s)	560
PvuII	CAGCTG	2 cut(s)	620, 756
RsaI	GTAC	6 cut(s)	725, 1051, 1222, 1333, 1724, 2033
RsaNI	GTAC	6 cut(s)	724, 1050, 1221, 1332, 1723, 2032
RseI	CAYNNNNRTG	3 cut(s)	602, 636, 1755
SacI	GAGCTC	2 cut(s)	960, 1464
SacII	CCGCGG	2 cut(s)	167, 193
SalI	GTCGAC	1 cut(s)	410
SaqAI	TTAA	3 cut(s)	819, 881, 2124
Sau3AI	GATC	7 cut(s)	497, 642, 1358, 1389, 1563, 1812, 2023
Sau96I	GGNCC	9 cut(s)	157, 438, 805, 914, 924, 1926, 1964, 2115, 2225
ScaI	AGTACT	2 cut(s)	725, 2033
SchI	GAGTC	2 cut(s)	1032, 2034
ScrFI	CCNGG	8 cut(s)	655, 889, 918, 981, 1379, 1553, 1930, 2373
SduI	GDGCHC	6 cut(s)	216, 453, 554, 960, 1464, 2178
SfaNI	GCATC	5 cut(s)	183, 273, 533, 1819, 2119
SfcI	CTRYAG	3 cut(s)	667, 1003, 2409
Sfr274I	CTCGAG	1 cut(s)	533
Sfr303I	CCGCGG	2 cut(s)	167, 193
SgrBI	CCGCGG	2 cut(s)	167, 193
SinI	GGWCC	2 cut(s)	914, 1926
SlaI	CTCGAG	1 cut(s)	533
SmiMI	CAYNNNNRTG	3 cut(s)	602, 636, 1755
SmlI	CTYRAG	1 cut(s)	533
SmoI	CTYRAG	1 cut(s)	533
SspMI	CTAG	2 cut(s)	1299, 2385
SstI	GAGCTC	2 cut(s)	960, 1464
StyD4I	CCNGG	8 cut(s)	653, 887, 916, 979, 1377, 1551, 1928, 2371
TaaI	ACNGT	9 cut(s)	139, 202, 358, 409, 424, 784, 1225, 1736, 2011
TaiI	ACGT	4 cut(s)	450, 1139, 1160, 1337
TatI	WGTACW	4 cut(s)	723, 1220, 1722, 2031
TfiI	GAWTC	4 cut(s)	944, 1270, 1625, 2243
Tru1I	TTAA	3 cut(s)	819, 881, 2124
Tru9I	TTAA	3 cut(s)	819, 881, 2124
TscAI	CASTG	2 cut(s)	1012, 2178
TseFI	GTSAC	1 cut(s)	735
TseI	GCWGC	5 cut(s)	169, 617, 684, 756, 1304
Tsp45I	GTSAC	1 cut(s)	735
TspGWI	ACGGA	2 cut(s)	351, 1341
TspRI	CASTG	2 cut(s)	1012, 2178
VpaK11BI	GGWCC	2 cut(s)	914, 1926
XapI	RAATTY	9 cut(s)	476, 516, 846, 892, 972, 1017, 1795, 2290, 2335
XceI	RCATGY	2 cut(s)	601, 635
XcmI	CCANNNNNNNNNTGG	1 cut(s)	658
XhoI	CTCGAG	1 cut(s)	533
XmiI	GTMKAC	2 cut(s)	411, 2270
XmnI	GAANNNNTTC	1 cut(s)	1481
XspI	CTAG	2 cut(s)	1299, 2385
ZraI	GACGTC	1 cut(s)	1137
ZrmI	AGTACT	2 cut(s)	725, 2033
Zsp2I	ATGCAT	1 cut(s)	2283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.