Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003366

Physical Location & Seq

Genomic Coordinates Forward (+)

5596720 .. 5599131

Gene Structure 2412 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1068800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2412 bp

ATGACACTTTCACATGAACCCTTCTCTCTCATCACCACCACAATCTTGATCACAACCTTCATATCTTCTCTCATCACACCACATAATTGGAGGGTGGCCAATGGCCTTGGCCTAGCCTCCACCACAGCCATCACATATGGCAATCCAACGGTTTGTGGCATTGTGGCTGAGAACCCCACACAGAGCATACAATGCTATCAAAACACTCAGACCACCATTTCAGTCCAACCCAATATTTCTTTTGAATCCATTTCTGGAGGCAAGAGCTTCTTCTGTGGCCTCAGGTCTGGAGGGTTTACCCTTCTCTGCTGGGAGACCAGCTTGTCTAGCTCAAATAGGTTTCGACCGAAACGAATATATCACAGTGAGAATGTAGCTTTGACTGATCTGGCTGTGGGTGATGAACAAGTTTGCGCCAGAGAAGTCAACTTTAGAATTGTCAGGTGCTGGAGAGGAAGAGATAATACTAGTGGGGTTTTGTTTCCCTCACCTGGGGAAGCATTGGAGTTTCGAACAATAGCATCGGGAAGAGGGTTTTCTTGTGGGATTTCGATGAATGATAGGAGAGTCCTGTGTTGGGGCCAGAGTGGTGTTGGAGATGATATTCAAACAGGGTTTGAGAATGTGAAAATGTCAAGCCTTGTTGCAGGAGAATCTCATGCTTGTGGCTTGAGTATGAATGGAACATTGGTCTGCAGAGGAAACAATGATTTCGGGCAATTGAATGTACCTTCAAGTACTTCGACTTTCCAGTTTTCAGGCCTTGCGTTGGGGGAAAATTTTACTTGTGCCATTCAGCGAAAAAATGGATTTGTTGTATGCTGGGGAGGAAGAAACAGATTTGAATACGACAGTGTTGTACTTACAAATGTTTCATTTGAGTTAATTTCTGCAGGTTTGGATTTCGTGTGCGGCGTGACAACGGGTAACTTATCAGTCATTTGTTGGGGACCAGGGTGGTCTGGTTCTCGTGATAACCTTCCACTGGGAATGATTCTTCCTGGTCCATGTGTGCAGGGTCCATGTACTAGCTGTGTGTACCCGAATTCGGAGACTCTTTGTGGTGGTTCAGGGAAAATATGCGGATCATGCCAGGTTGAACTCCCATTAGCACTTCCATTGCCTCCAATAAATCAACCTACCCAAGCTTCAGAGCCGGGTTTTTCGTTATCCATAACAAGAAATAAGCTTCTGTTGGTTTTTGTAATAGTTGGATCAGTTGGGGCATTTGCAGGACTCTGCACTATTGTCTTTTGCCTATGGACTGGACTGTGTGGTTCTTGGTTAAACCGTCATGACTCTGTGCAAACTGAAAGTTTTACTGATCCAAATGTGGGTTCTTCTGCTGCTAGAATCGCCAATGTTCCTAATGCTCCATTAAGTTCAAGTTCCATGAAGGGCAGTTTAAGTGGTTCATCATCAAAGCACGGTGACAAGACTCAGTCATTTCACCTGGCAGAACTTTCTACTGCCACCAAACATTTCTCAACCGAAAACAAGATCGGTGCTGGGAGCTTTGGCATTGTCTATAAAGGCAAGCTTGCGGATGGTCGTGAAGTGGCCATCAAGAGGGGAAATACAAGTACCAAGACCAAGAAATTTCAGGAGAAAGAGAGCGCGTTTGACTCCGAATTAGCATTGTTGTCCCGGCTTCACCACAAGCACTTGGTGAAGCTAGTGGGATCCTGTGAAGTTAACGATGAGAGGCTTTTGGTTTACGAGTACATGAGCAACGGTTCGCTTCATGATCACTTGCATGGCAAGAACAATGTGCAGGAAAATAGCTGCATTGTGAATTCTTGGAAAATGAGGATAAAAATTGCGCTTGATGCAGCCAGGGGAATAGAGTACCTTCACAATTATGCAGTGCCAACAATAATTCACAGGGACATCAAGTCCTCAAACATTCTTTTGGATGCAAATTGGAATGCAAGGGTATCCGATTTTGGACTGTCATTATTGGGGCCGGAATCAGACCAAGAGATCATGTCGTCCAAAGCGGTTGGAACAGTTGGGTACATTGATCCCGAGTACTTTGTGTCAAACGTTTTGACAGCAAAGAGTGACGTATACGGATTTGGAGTGGTGCTATTGGAGCTTTTGACAGGGAAGAGGGCTCTGTTTATAAATAGTGAAGACGGAGGTCCAATGGGGGTTGTGGAATACGCAGGGCCGCGAATAGTGGCAGGGCAGCTGCAAAGCATGTTGGATCAAAGGGTGGGGAAGCCGGACCCGAAGGAAGGCGAAGCTGTTGAGCTAGTGGCTTATACTGCTATGCATTGTGTGAACTTGGAGGGGAAGGAACGGCCTAGCATGACTGACATTGTTGCCAATTTAGAGAGGGCTCTTGCTCTTTGCGAGGATGATCCTTTTAGCTTCTCTACTACCACAATTTCCTTCCCCTCGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0042802 GO:0042803 GO:0046983

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase RCC1_2

Protein Analysis

804

Amino Acids

86.52

Weight (kDa)

6.21

Isoelectric Point (pI)

34.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RCC1_2	PF13540	213 - 240	6.6e-06	Regulator of chromosome condensation (RCC1) repeat
Pkinase	PF00069	496 - 713	5.7e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	497 - 779	6.6e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55950
fragaria_vesca	FvH4_3g38710 FvH4_7g24490
malus_domestica	MD00G1166500.v1.1 MD01G1153100.v1.1 MD03G1068800.v1.1 MD07G1222100.v1.1 MD11G1073300.v1.1
prunus_persica	Prupe.2G250300_v2.0.a1 Prupe.6G056600_v2.0.a1
pyrus_communis	pycom01g17070 pycom03g05510
rosa_chinensis	RchiOBHm_Chr1g0370251 RchiOBHm_Chr5g0065501 RchiOBHm_Chr5g0069861
rosa_laevigata	RLG00000027065 RLG00000036103
rosa_multiflora	Rmu_co8321277.1_g000001 Rmu_sc0000738.1_g000002 Rmu_sc0026893.1_g000001 Rmu_sc0037931.1_g000001
rosa_roxburghii	Rroxscaffold_1G00011190 Rroxscaffold_4G00286710 Rroxscaffold_4G00287300
rosa_rugosa	Rorug01G0355200 Rorug05G0400300
rosa_samantha	Rh1AG363800 Rh1BG326900 Rh1CG340200 Rh1DG357300 Rh5AG457400 Rh5BG476200 Rh5CG500000 Rh5DG488000
rosa_wichuraiana	Rw1G032010 Rw5G042700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2126
AasI	GACNNNNNNGTC	1 cut(s)	322
Acc36I	ACCTGC	1 cut(s)	884
AccI	GTMKAC	1 cut(s)	2070
AccII	CGCG	2 cut(s)	1619, 2176
AciI	CCGC	5 cut(s)	912, 1083, 1544, 2000, 2174
AclI	AACGTT	1 cut(s)	2046
AclWI	GGATC	8 cut(s)	1093, 1222, 1319, 1677, 1690, 2018, 2217, 2360
AcoI	YGGCCR	2 cut(s)	96, 1560
AcsI	RAATTY	4 cut(s)	778, 1045, 1598, 1795
AcuI	CTGAAG	1 cut(s)	1134
AdeI	CACNNNGTG	1 cut(s)	1669
AfiI	CCNNNNNNNGG	9 cut(s)	491, 492, 577, 769, 985, 1048, 1334, 1569, 2240
AgsI	TTSAA	7 cut(s)	245, 608, 724, 735, 845, 1100, 1386
AhdI	GACNNNNNGTC	1 cut(s)	1894
AhlI	ACTAGT	1 cut(s)	467
AjnI	CCWGG	6 cut(s)	490, 952, 1000, 1092, 1452, 1835
AjuI	GAANNNNNNNTTGG	4 cut(s)	671, 703, 1321, 1353
Alw26I	GTCTC	2 cut(s)	308, 1046
AlwI	GGATC	8 cut(s)	1093, 1222, 1319, 1677, 1690, 2018, 2217, 2360
AlwNI	CAGNNNCTG	1 cut(s)	447
Ama87I	CYCGRG	1 cut(s)	2027
ApeKI	GCWGC	5 cut(s)	1346, 1785, 1832, 2191, 2194
ApoI	RAATTY	4 cut(s)	778, 1045, 1598, 1795
AspLEI	GCGC	3 cut(s)	416, 1619, 1825
AspS9I	GGNCC	8 cut(s)	580, 950, 1004, 1019, 1964, 2144, 2171, 2230
AsuC2I	CCSGG	2 cut(s)	1158, 1648
AsuHPI	GGTGA	7 cut(s)	25, 410, 480, 1442, 1442, 1646, 1681
AsuII	TTCGAA	1 cut(s)	511
AvaI	CYCGRG	1 cut(s)	2027
AvaII	GGWCC	5 cut(s)	950, 1004, 1019, 2144, 2230
AxyI	CCTNAGG	1 cut(s)	281
BalI	TGGCCA	2 cut(s)	98, 1562
BamHI	GGATCC	1 cut(s)	1682
BanII	GRGCYC	2 cut(s)	2119, 2347
BarI	GAAGNNNNNNTAC	2 cut(s)	448, 480
BauI	CACGAG	1 cut(s)	969
BbsI	GAAGAC	1 cut(s)	2142
BbvI	GCAGC	5 cut(s)	1333, 1772, 1844, 2181, 2203
BccI	CCATC	3 cut(s)	137, 1541, 1571
BceAI	ACGGC	1 cut(s)	2321
BciT130I	CCWGG	6 cut(s)	492, 954, 1002, 1094, 1454, 1837
BciVI	GTATCC	1 cut(s)	1948
BclI	TGATCA	2 cut(s)	48, 1747
BcnI	CCSGG	2 cut(s)	1158, 1648
BcoDI	GTCTC	2 cut(s)	308, 1046
BcuI	ACTAGT	1 cut(s)	467
BfaI	CTAG	8 cut(s)	113, 327, 468, 1029, 1350, 1676, 2258, 2310
BfmI	CTRYAG	2 cut(s)	694, 891
BfuAI	ACCTGC	1 cut(s)	884
BfuI	GTATCC	1 cut(s)	1948
BisI	GCNGC	7 cut(s)	913, 1347, 1786, 1833, 2174, 2192, 2195
BlsI	GCNGC	7 cut(s)	914, 1348, 1787, 1834, 2175, 2193, 2196
BmcAI	AGTACT	2 cut(s)	739, 2033
Bme1390I	CCNGG	8 cut(s)	492, 954, 1002, 1094, 1158, 1454, 1648, 1837
Bme18I	GGWCC	5 cut(s)	950, 1004, 1019, 2144, 2230
BmeRI	GACNNNNNGTC	1 cut(s)	1894
BmeT110I	CYCGRG	1 cut(s)	2027
BmgT120I	GGNCC	8 cut(s)	580, 950, 1004, 1019, 1964, 2144, 2171, 2230
BmiI	GGNNCC	6 cut(s)	581, 951, 1020, 1684, 1965, 2232
BmrFI	CCNGG	8 cut(s)	492, 954, 1002, 1094, 1158, 1454, 1648, 1837
BmrI	ACTGGG	1 cut(s)	995
BmsI	GCATC	3 cut(s)	530, 1819, 1906
BmuI	ACTGGG	1 cut(s)	995
BoxI	GACNNNNGTC	1 cut(s)	2142
BpiI	GAAGAC	1 cut(s)	2142
BpmI	CTGGAG	3 cut(s)	276, 309, 469
Bpu14I	TTCGAA	1 cut(s)	511
BpuEI	CTTGAG	1 cut(s)	691
BpuMI	CCSGG	2 cut(s)	1158, 1648
BsaI	GGTCTC	1 cut(s)	308
BsaJI	CCNNGG	4 cut(s)	106, 491, 953, 1836
Bsc4I	CCNNNNNNNGG	9 cut(s)	491, 492, 577, 769, 985, 1048, 1334, 1569, 2240
Bse1I	ACTGG	3 cut(s)	751, 990, 1270
Bse21I	CCTNAGG	1 cut(s)	281
Bse3DI	GCAATG	1 cut(s)	1118
BseBI	CCWGG	6 cut(s)	492, 954, 1002, 1094, 1454, 1837
BseDI	CCNNGG	4 cut(s)	106, 491, 953, 1836
BseGI	GGATG	3 cut(s)	1552, 1921, 2368
BseLI	CCNNNNNNNGG	9 cut(s)	491, 492, 577, 769, 985, 1048, 1334, 1569, 2240
BseMI	GCAATG	1 cut(s)	1118
BseMII	CTCAG	4 cut(s)	159, 221, 295, 1454
BseNI	ACTGG	3 cut(s)	751, 990, 1270
BseXI	GCAGC	5 cut(s)	1333, 1772, 1844, 2181, 2203
BseYI	CCCAGC	3 cut(s)	309, 822, 1508
BsgI	GTGCAG	3 cut(s)	1034, 1225, 1793
Bsh1236I	CGCG	2 cut(s)	1619, 2176
Bsh1285I	CGRYCG	1 cut(s)	347
BsiEI	CGRYCG	1 cut(s)	347
BsiHKCI	CYCGRG	1 cut(s)	2027
BsiSI	CCGG	4 cut(s)	1157, 1648, 1967, 2228
BslFI	GGGAC	3 cut(s)	963, 1630, 1901
BslI	CCNNNNNNNGG	9 cut(s)	491, 492, 577, 769, 985, 1048, 1334, 1569, 2240
BsmAI	GTCTC	2 cut(s)	308, 1046
BsmFI	GGGAC	3 cut(s)	963, 1630, 1901
BsmI	GAATGC	1 cut(s)	1933
Bso31I	GGTCTC	1 cut(s)	308
BsoBI	CYCGRG	1 cut(s)	2027
Bsp119I	TTCGAA	1 cut(s)	511
Bsp1286I	GDGCHC	2 cut(s)	2119, 2347
BspACI	CCGC	5 cut(s)	912, 1083, 1544, 2000, 2174
BspCNI	CTCAG	4 cut(s)	160, 220, 294, 1453
BspFNI	CGCG	2 cut(s)	1619, 2176
BspHI	TCATGA	2 cut(s)	1294, 1744
BspLI	GGNNCC	6 cut(s)	581, 951, 1020, 1684, 1965, 2232
BspMAI	CTGCAG	2 cut(s)	698, 895
BspMI	ACCTGC	1 cut(s)	884
BspPI	GGATC	8 cut(s)	1093, 1222, 1319, 1677, 1690, 2018, 2217, 2360
BspT104I	TTCGAA	1 cut(s)	511
BspTNI	GGTCTC	1 cut(s)	308
BsrDI	GCAATG	1 cut(s)	1118
BsrI	ACTGG	3 cut(s)	751, 990, 1270
BssECI	CCNNGG	4 cut(s)	106, 491, 953, 1836
BssNAI	GTATAC	1 cut(s)	2071
BssSI	CACGAG	1 cut(s)	969
BssT1I	CCWWGG	1 cut(s)	106
Bst1107I	GTATAC	1 cut(s)	2071
Bst2BI	CACGAG	1 cut(s)	969
Bst2UI	CCWGG	6 cut(s)	492, 954, 1002, 1094, 1454, 1837
Bst4CI	ACNGT	9 cut(s)	151, 365, 854, 1272, 1292, 1430, 1736, 1953, 2011
Bst6I	CTCTTC	3 cut(s)	451, 523, 2105
BstAPI	GCANNNNNTGC	1 cut(s)	192
BstBI	TTCGAA	1 cut(s)	511
BstC8I	GCNNGC	2 cut(s)	1538, 1542
BstDEI	CTNAG	4 cut(s)	168, 207, 281, 1440
BstF5I	GGATG	3 cut(s)	1552, 1921, 2368
BstFNI	CGCG	2 cut(s)	1619, 2176
BstHHI	GCGC	3 cut(s)	416, 1619, 1825
BstMAI	GTCTC	2 cut(s)	308, 1046
BstMCI	CGRYCG	1 cut(s)	347
BstMWI	GCNNNNNNNGC	7 cut(s)	192, 327, 1089, 1355, 1829, 2095, 2270
BstNI	CCWGG	6 cut(s)	492, 954, 1002, 1094, 1454, 1837
BstNSI	RCATGY	1 cut(s)	2206
BstPAI	GACNNNNGTC	1 cut(s)	2142
BstSCI	CCNGG	8 cut(s)	490, 952, 1000, 1092, 1156, 1452, 1646, 1835
BstSFI	CTRYAG	2 cut(s)	694, 891
BstUI	CGCG	2 cut(s)	1619, 2176
BstV1I	GCAGC	5 cut(s)	1333, 1772, 1844, 2181, 2203
BstV2I	GAAGAC	1 cut(s)	2142
BstX2I	RGATCY	1 cut(s)	1682
BstXI	CCANNNNNNTGG	1 cut(s)	87
BstYI	RGATCY	1 cut(s)	1682
BstZ17I	GTATAC	1 cut(s)	2071
Bsu36I	CCTNAGG	1 cut(s)	281
BsuI	GTATCC	1 cut(s)	1948
BtsCI	GGATG	3 cut(s)	1552, 1921, 2368
BtsI	GCAGTG	1 cut(s)	1872
BtsIMutI	CAGTG	4 cut(s)	370, 859, 983, 1872
BveI	ACCTGC	1 cut(s)	884
Cac8I	GCNNGC	2 cut(s)	1538, 1542
CaiI	CAGNNNCTG	1 cut(s)	447
CciI	TCATGA	2 cut(s)	1294, 1744
CfoI	GCGC	3 cut(s)	416, 1619, 1825
Cfr13I	GGNCC	8 cut(s)	580, 950, 1004, 1019, 1964, 2144, 2171, 2230
DdeI	CTNAG	4 cut(s)	168, 207, 281, 1440
DraIII	CACNNNGTG	1 cut(s)	1669
DrdI	GACNNNNNNGTC	1 cut(s)	322
DriI	GACNNNNNGTC	1 cut(s)	1894
DseDI	GACNNNNNNGTC	1 cut(s)	322
EaeI	YGGCCR	2 cut(s)	96, 1560
Eam1104I	CTCTTC	3 cut(s)	451, 523, 2105
Eam1105I	GACNNNNNGTC	1 cut(s)	1894
EarI	CTCTTC	3 cut(s)	451, 523, 2105
Eco130I	CCWWGG	1 cut(s)	106
Eco147I	AGGCCT	1 cut(s)	762
Eco24I	GRGCYC	2 cut(s)	2119, 2347
Eco31I	GGTCTC	1 cut(s)	308
Eco47I	GGWCC	5 cut(s)	950, 1004, 1019, 2144, 2230
Eco57I	CTGAAG	1 cut(s)	1134
Eco81I	CCTNAGG	1 cut(s)	281
Eco88I	CYCGRG	1 cut(s)	2027
EcoRI	GAATTC	2 cut(s)	1045, 1795
EcoRII	CCWGG	6 cut(s)	490, 952, 1000, 1092, 1452, 1835
EcoT14I	CCWWGG	1 cut(s)	106
EcoT22I	ATGCAT	1 cut(s)	2280
EcoT38I	GRGCYC	2 cut(s)	2119, 2347
ErhI	CCWWGG	1 cut(s)	106
FalI	AAGNNNNNCTT	4 cut(s)	254, 286, 1524, 1556
FaqI	GGGAC	3 cut(s)	963, 1630, 1901
FauNDI	CATATG	1 cut(s)	136
FbaI	TGATCA	2 cut(s)	48, 1747
FblI	GTMKAC	1 cut(s)	2070
Fnu4HI	GCNGC	7 cut(s)	913, 1347, 1786, 1833, 2174, 2192, 2195
FokI	GGATG	3 cut(s)	1559, 1928, 2375
FriOI	GRGCYC	2 cut(s)	2119, 2347
Fsp4HI	GCNGC	7 cut(s)	913, 1347, 1786, 1833, 2174, 2192, 2195
FspBI	CTAG	8 cut(s)	113, 327, 468, 1029, 1350, 1676, 2258, 2310
GlaI	GCGC	3 cut(s)	415, 1618, 1824
GluI	GCNGC	7 cut(s)	913, 1347, 1786, 1833, 2174, 2192, 2195
GsaI	CCCAGC	3 cut(s)	313, 826, 1512
GsuI	CTGGAG	3 cut(s)	276, 309, 469
HapII	CCGG	4 cut(s)	1157, 1648, 1967, 2228
HhaI	GCGC	3 cut(s)	416, 1619, 1825
Hin6I	GCGC	3 cut(s)	414, 1617, 1823
HinP1I	GCGC	3 cut(s)	414, 1617, 1823
HincII	GTYRAC	2 cut(s)	427, 1696
HindII	GTYRAC	2 cut(s)	427, 1696
HindIII	AAGCTT	3 cut(s)	1146, 1187, 1538
HpaI	GTTAAC	1 cut(s)	1696
HpaII	CCGG	4 cut(s)	1157, 1648, 1967, 2228
HphI	GGTGA	7 cut(s)	25, 410, 480, 1442, 1442, 1646, 1681
Hpy166II	GTNNAC	7 cut(s)	297, 427, 1039, 1696, 1717, 2071, 2287
Hpy188I	TCNGA	6 cut(s)	210, 1051, 1153, 1630, 1942, 1975
Hpy8I	GTNNAC	7 cut(s)	297, 427, 1039, 1696, 1717, 2071, 2287
HpyCH4III	ACNGT	9 cut(s)	151, 365, 854, 1272, 1292, 1430, 1736, 1953, 2011
HpyCH4IV	ACGT	2 cut(s)	2046, 2067
HpyF10VI	GCNNNNNNNGC	7 cut(s)	192, 327, 1089, 1355, 1829, 2095, 2270
HpyF3I	CTNAG	4 cut(s)	168, 207, 281, 1440
HpySE526I	ACGT	2 cut(s)	2046, 2067
HspAI	GCGC	3 cut(s)	414, 1617, 1823
Ksp22I	TGATCA	2 cut(s)	48, 1747
KspAI	GTTAAC	1 cut(s)	1696
LmnI	GCTCC	3 cut(s)	1378, 1512, 2095
Lsp1109I	GCAGC	5 cut(s)	1333, 1772, 1844, 2181, 2203
LweI	GCATC	3 cut(s)	530, 1819, 1906
MaeI	CTAG	8 cut(s)	113, 327, 468, 1029, 1350, 1676, 2258, 2310
MaeII	ACGT	2 cut(s)	2046, 2067
MaeIII	GTNAC	4 cut(s)	916, 926, 1430, 2063
MboII	GAAGA	9 cut(s)	57, 262, 468, 540, 843, 989, 1332, 2122, 2147
MfeI	CAATTG	1 cut(s)	719
MflI	RGATCY	1 cut(s)	1682
MhlI	GDGCHC	2 cut(s)	2119, 2347
MlsI	TGGCCA	2 cut(s)	98, 1562
MluNI	TGGCCA	2 cut(s)	98, 1562
MlyI	GAGTC	6 cut(s)	576, 1048, 1230, 1292, 1432, 1619
MmeI	TCCRAC	6 cut(s)	170, 250, 574, 1192, 1984, 2187
Mox20I	TGGCCA	2 cut(s)	98, 1562
Mph1103I	ATGCAT	1 cut(s)	2280
MscI	TGGCCA	2 cut(s)	98, 1562
MseI	TTAA	5 cut(s)	884, 1286, 1379, 1406, 1695
MslI	CAYNNNNRTG	3 cut(s)	663, 1755, 1860
Msp20I	TGGCCA	2 cut(s)	98, 1562
MspA1I	CMGCKG	1 cut(s)	2194
MspI	CCGG	4 cut(s)	1157, 1648, 1967, 2228
MspR9I	CCNGG	8 cut(s)	492, 954, 1002, 1094, 1158, 1454, 1648, 1837
MunI	CAATTG	1 cut(s)	719
Mva1269I	GAATGC	1 cut(s)	1933
MvaI	CCWGG	6 cut(s)	492, 954, 1002, 1094, 1454, 1837
MvnI	CGCG	2 cut(s)	1619, 2176
MwoI	GCNNNNNNNGC	7 cut(s)	192, 327, 1089, 1355, 1829, 2095, 2270
NciI	CCSGG	2 cut(s)	1158, 1648
NdeI	CATATG	1 cut(s)	136
NlaIV	GGNNCC	6 cut(s)	581, 951, 1020, 1684, 1965, 2232
NmuCI	GTSAC	3 cut(s)	916, 1430, 2063
NsiI	ATGCAT	1 cut(s)	2280
NspI	RCATGY	1 cut(s)	2206
NspV	TTCGAA	1 cut(s)	511
PagI	TCATGA	2 cut(s)	1294, 1744
PceI	AGGCCT	1 cut(s)	762
PcsI	WCGNNNNNNNCGW	2 cut(s)	349, 912
PctI	GAATGC	1 cut(s)	1933
PfeI	GAWTC	5 cut(s)	245, 653, 994, 1353, 1970
PflFI	GACNNNGTC	1 cut(s)	1441
PkrI	GCNGC	7 cut(s)	914, 1348, 1787, 1834, 2175, 2193, 2196
PleI	GAGTC	6 cut(s)	575, 1048, 1230, 1292, 1432, 1619
PpsI	GAGTC	6 cut(s)	575, 1048, 1230, 1292, 1432, 1619
PshAI	GACNNNNGTC	1 cut(s)	2142
PsiI	TTATAA	1 cut(s)	2126
Psp1406I	AACGTT	1 cut(s)	2046
Psp6I	CCWGG	6 cut(s)	490, 952, 1000, 1092, 1452, 1835
PspFI	CCCAGC	3 cut(s)	309, 822, 1508
PspGI	CCWGG	6 cut(s)	490, 952, 1000, 1092, 1452, 1835
PspN4I	GGNNCC	6 cut(s)	581, 951, 1020, 1684, 1965, 2232
PspPI	GGNCC	8 cut(s)	580, 950, 1004, 1019, 1964, 2144, 2171, 2230
PstI	CTGCAG	2 cut(s)	698, 895
PstNI	CAGNNNCTG	1 cut(s)	447
PsuI	RGATCY	1 cut(s)	1682
PsyI	GACNNNGTC	1 cut(s)	1441
PvuII	CAGCTG	1 cut(s)	2194
RseI	CAYNNNNRTG	3 cut(s)	663, 1755, 1860
SaqAI	TTAA	5 cut(s)	884, 1286, 1379, 1406, 1695
SatI	GCNGC	7 cut(s)	913, 1347, 1786, 1833, 2174, 2192, 2195
Sau96I	GGNCC	8 cut(s)	580, 950, 1004, 1019, 1964, 2144, 2171, 2230
ScaI	AGTACT	2 cut(s)	739, 2033
SchI	GAGTC	6 cut(s)	576, 1048, 1230, 1292, 1432, 1619
ScrFI	CCNGG	8 cut(s)	492, 954, 1002, 1094, 1158, 1454, 1648, 1837
SduI	GDGCHC	2 cut(s)	2119, 2347
SfaNI	GCATC	3 cut(s)	530, 1819, 1906
SfcI	CTRYAG	2 cut(s)	694, 891
SfuI	TTCGAA	1 cut(s)	511
SinI	GGWCC	5 cut(s)	950, 1004, 1019, 2144, 2230
SmiMI	CAYNNNNRTG	3 cut(s)	663, 1755, 1860
SmlI	CTYRAG	1 cut(s)	670
SmoI	CTYRAG	1 cut(s)	670
SpeI	ACTAGT	1 cut(s)	467
SseBI	AGGCCT	1 cut(s)	762
SsiI	CCGC	5 cut(s)	912, 1083, 1544, 2000, 2174
SspI	AATATT	1 cut(s)	235
SspMI	CTAG	8 cut(s)	113, 327, 468, 1029, 1350, 1676, 2258, 2310
StuI	AGGCCT	1 cut(s)	762
StyD4I	CCNGG	8 cut(s)	490, 952, 1000, 1092, 1156, 1452, 1646, 1835
StyI	CCWWGG	1 cut(s)	106
TaaI	ACNGT	9 cut(s)	151, 365, 854, 1272, 1292, 1430, 1736, 1953, 2011
TaiI	ACGT	2 cut(s)	2049, 2070
TaqI	TCGA	4 cut(s)	343, 511, 551, 743
TaqII	GACCGA	1 cut(s)	361
TatI	WGTACW	5 cut(s)	737, 859, 1025, 1722, 2031
TauI	GCSGC	2 cut(s)	915, 2176
TfiI	GAWTC	5 cut(s)	245, 653, 994, 1353, 1970
Tru1I	TTAA	5 cut(s)	884, 1286, 1379, 1406, 1695
Tru9I	TTAA	5 cut(s)	884, 1286, 1379, 1406, 1695
TscAI	CASTG	4 cut(s)	370, 859, 990, 1872
TseFI	GTSAC	3 cut(s)	916, 1430, 2063
TseI	GCWGC	5 cut(s)	1346, 1785, 1832, 2191, 2194
Tsp45I	GTSAC	3 cut(s)	916, 1430, 2063
TspDTI	ATGAA	9 cut(s)	30, 49, 417, 569, 692, 864, 1404, 1409, 1733
TspGWI	ACGGA	2 cut(s)	2088, 2154
TspRI	CASTG	4 cut(s)	370, 859, 990, 1872
Tth111I	GACNNNGTC	1 cut(s)	1441
VpaK11BI	GGWCC	5 cut(s)	950, 1004, 1019, 2144, 2230
XapI	RAATTY	4 cut(s)	778, 1045, 1598, 1795
XceI	RCATGY	1 cut(s)	2206
XcmI	CCANNNNNNNNNTGG	1 cut(s)	590
XmiI	GTMKAC	1 cut(s)	2070
XspI	CTAG	8 cut(s)	113, 327, 468, 1029, 1350, 1676, 2258, 2310
ZrmI	AGTACT	2 cut(s)	739, 2033
Zsp2I	ATGCAT	1 cut(s)	2280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.