Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002464

Physical Location & Seq

Genomic Coordinates Reverse (-)

19730920 .. 19736391

Gene Structure 5472 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1087800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGCCTTTGTTATACACAAACTCAGTTAAGTTGGGGATCCTTCTCCGATTAATTTGTCTATTCATCTTGTCATTCTCGGTGTGCATTTCAACCCCTCCAGAGGACCACATAAAGTGTTCATCATCAACCAACACAAACTGCGCCATCACAAACTCCTACGGCATCTTCCCCGATCGATCGGTTTGTCGAGCAGGCAGCGTAGCATATCCTTCGGACGAGGAAGAACTAATTTCTGTAGTGGCCAATGCAGCAAAGAAGAAGACCAAAATGAAGGTGGCAACTCGTTACTCTCATAGCATTCCAAAGCTGGTTTGTCCAAGTGGGGAAGATGGGTTGATCATAAGCACCAAGTACCTCAATCGTGTGGTGGAGATTGATGCTGAAGCAAAGACGATGACATTGGAGAGTGGTGTGACTCTTAGGCAGCTCATCAGTGAGGCTGCCAAGGCAGGGCTGGCCTTGCCTTATACACCATATTGGTGGGGTTTAACCGTGGGAGGTTTATTAGGGACTGGGGCACATGGTAGTACGTTGTGGGGCAATGGAAGCTCGATTCATGATTATGTGGTGGGACTTACAATTGTGAGTCCAGGAGGACCTGGGGATGGTTATGTCAAGGTCCGAAGGCTCAATGATGGCGATAGAGATCTTAATGCAGCTAAGGTTTCACTTGGAGTCCTAGGAGTAATTTCCCAGGTTACTCTAAATTTGCAACCCATGTTTAAGCGATCCATCACTTATCTGAGCAAGGACGATTCAGATTTGGGAGACCAAGTAGTTAGGTTTGGCAAGGAACACGAGTTTGCAGACATCCTATGGTACCCTAGCCAGCACAAGGCAGTCTATCGGATGGATGATCGAGTCTCCACCAATACATCTGGAAATGGCTTATATGACTTCATTCCTTTTCGAGCCACACCTTCACTTGCTCTTGCAGTTGTCAGAACCACAGAGGAGAATCAAGAATCCAAGAGTGATGCTGATGGGAAATGCACTGGTGCGAAGTTGGTTACATCTACACTCATTAAAAGTGCTTATGGTTTAACAAACAATGGTCTGATTTTTACTGGCTATCCTGTTATTGGATATCAGCACCGGCTTCAATCATCAGGGACTTGCCTAGATAGTCTTGAGGATTCAAGAATCACAGCGTGCGCATGGGATCCCAAAATCAAGGGTGAGTTTTTTCATCAAACAACGTTCAGCATCAACTTGTCTCTGGCTAAGAACTTCATCCAAGATGTGCAAAAGCTGGTTGAGATTGAACCAAAAGCGCTATGTGGAGTAGAAATCTACAATGGAATCCTAATGCGGTATGTTACAACCTCAAGCGCTTACCTTGGCAAGCAAAAAGATGCAATCGACTTCGATATCACATATTATCGAAGTAAAGACCCTATGACTCCTAGACTCCATGAAGGAATACTTGAAGAAATAGAGCAACTTGCTCTTGTTAAGTATGGAGCATTGCCTCACTGGGGAAAAAATCGGAACATAGCATTTGATGGAGTGATGAACAAGTACAAGGGTGCTAAAGAGTTCTTGAGGGTAAAAAATGTTTACGATCCAATGGGGCTATTCTCTAGTGAATGGACAGATCAAGTTCTTGGACTTAAGGATGGGGTGACTATAGTAAAGGAAGGGTGTGCACTAGAAGGGTTGTGCATCTGCTCAAAAGACATTCACTGCGCCCCGAGCAAAGGCTATTTCTGTAGGCCTGGCAAAATCTTCAACGATGCAAGGATCTGTACCCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005618 GO:0005623 GO:0005975 GO:0005996 GO:0006082 GO:0006732 GO:0006766 GO:0006767 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016051 GO:0016053 GO:0016491 GO:0016614 GO:0016899 GO:0019752 GO:0019852 GO:0019853 GO:0030312 GO:0036094 GO:0042364 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044464 GO:0046364 GO:0046394 GO:0048037 GO:0050105 GO:0050660 GO:0050662 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:0071944 GO:0097159 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ALO FAD_binding_4

Protein Analysis

586

Amino Acids

64.22

Weight (kDa)

8.02

Isoelectric Point (pI)

31.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	67 - 199	1.5e-20	FAD binding domain
ALO	PF04030	396 - 534	5.2e-10	D-arabinono-1,4-lactone oxidase
GULLO2-like_3rd	PF22906	546 - 584	2.1e-23	L-gulonolactone oxidase 2-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46750 AT2G46760 AT5G56470
fragaria_vesca	FvH4_7g17510 FvH4_7g17511
malus_domestica	MD01G1087800.v1.1 MD07G1157900.v1.1
prunus_persica	Prupe.2G196400_v2.0.a1
pyrus_communis	pycom01g11170 pycom07g15450
rosa_chinensis	RchiOBHm_Chr1g0360921 RchiOBHm_Chr1g0360951 RchiOBHm_Chr3g0481291 RchiOBHm_Chr5g0029651
rosa_laevigata	RLG00000019622 RLG00000027767 RLG00000027769
rosa_multiflora	Rmu_sc0000206.1_g000019 Rmu_sc0000677.1_g000024 Rmu_sc0002207.1_g000003 Rmu_sc0008012.1_g000002
rosa_roxburghii	Rroxscaffold_3G00228090 Rroxscaffold_4G00295680 Rroxscaffold_4G00295700 Rroxscaffold_5G00385060
rosa_rugosa	Rorug01G0283500 Rorug01G0283800 Rorug03G0191000
rosa_samantha	Rh1AG295000 Rh1BG259800 Rh1BG259900 Rh1CG277200 Rh1DG289900 Rh2BG393900 Rh3AG241900 Rh3BG277100 Rh3DG268900
rosa_wichuraiana	Rw1G026070 Rw1G026100 Rw1G026110 Rw3G021610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1156
Acc65I	GGTACC	1 cut(s)	819
AccB1I	GGYRCC	1 cut(s)	819
AciI	CCGC	1 cut(s)	1312
AclI	AACGTT	1 cut(s)	1199
AclWI	GGATC	7 cut(s)	31, 44, 723, 1157, 1170, 1559, 1751
AcoI	YGGCCR	1 cut(s)	240
AcsI	RAATTY	1 cut(s)	706
AcuI	CTGAAG	1 cut(s)	402
AdeI	CACNNNGTG	1 cut(s)	1152
AfaI	GTAC	5 cut(s)	353, 529, 821, 1523, 1750
AfeI	AGCGCT	2 cut(s)	1275, 1333
AfiI	CCNNNNNNNGG	7 cut(s)	100, 450, 605, 835, 1082, 1478, 1700
AflII	CTTAAG	1 cut(s)	1613
AgsI	TTSAA	6 cut(s)	90, 1103, 1140, 1265, 1430, 1732
AjnI	CCWGG	4 cut(s)	589, 598, 693, 1717
AluBI	AGCT	5 cut(s)	307, 427, 549, 659, 1252
AluI	AGCT	5 cut(s)	307, 427, 549, 659, 1252
Alw21I	GWGCWC	1 cut(s)	1651
Alw26I	GTCTC	3 cut(s)	762, 868, 1221
Alw44I	GTGCAC	1 cut(s)	1647
AlwI	GGATC	7 cut(s)	31, 44, 723, 1157, 1170, 1559, 1751
Ama87I	CYCGRG	1 cut(s)	1693
Aor51HI	AGCGCT	2 cut(s)	1275, 1333
AoxI	GGCC	3 cut(s)	240, 456, 1715
ApaLI	GTGCAC	1 cut(s)	1647
ApeKI	GCWGC	5 cut(s)	195, 248, 424, 440, 656
ApoI	RAATTY	1 cut(s)	706
AseI	ATTAAT	1 cut(s)	50
Asp718I	GGTACC	1 cut(s)	819
AspA2I	CCTAGG	1 cut(s)	679
AspLEI	GCGC	5 cut(s)	143, 1157, 1276, 1334, 1691
AspS9I	GGNCC	3 cut(s)	103, 596, 619
AsuHPI	GGTGA	2 cut(s)	1190, 1636
AvaI	CYCGRG	1 cut(s)	1693
AvaII	GGWCC	3 cut(s)	103, 596, 619
AvrII	CCTAGG	1 cut(s)	679
BaeGI	GKGCMC	2 cut(s)	520, 1651
BalI	TGGCCA	1 cut(s)	242
BamHI	GGATCC	2 cut(s)	36, 1162
BanI	GGYRCC	1 cut(s)	819
BauI	CACGAG	1 cut(s)	797
BbsI	GAAGAC	1 cut(s)	266
Bbv12I	GWGCWC	1 cut(s)	1651
BbvI	GCAGC	5 cut(s)	207, 260, 427, 436, 668
BccI	CCATC	9 cut(s)	152, 323, 599, 629, 740, 844, 977, 1499, 1613
BceAI	ACGGC	1 cut(s)	175
BcgI	CGANNNNNNTGC	2 cut(s)	192, 226
BciT130I	CCWGG	4 cut(s)	591, 600, 695, 1719
BclI	TGATCA	1 cut(s)	336
BcoDI	GTCTC	3 cut(s)	762, 868, 1221
BfaI	CTAG	6 cut(s)	680, 825, 1121, 1407, 1584, 1652
BfmI	CTRYAG	3 cut(s)	234, 1629, 1711
BfoI	RGCGCY	2 cut(s)	1277, 1335
BfrI	CTTAAG	1 cut(s)	1613
BglII	AGATCT	1 cut(s)	646
BisI	GCNGC	5 cut(s)	196, 249, 425, 441, 657
BlnI	CCTAGG	1 cut(s)	679
BlsI	GCNGC	5 cut(s)	197, 250, 426, 442, 658
Bme1390I	CCNGG	4 cut(s)	591, 600, 695, 1719
Bme18I	GGWCC	3 cut(s)	103, 596, 619
BmeT110I	CYCGRG	1 cut(s)	1693
BmgT120I	GGNCC	3 cut(s)	103, 596, 619
BmiI	GGNNCC	3 cut(s)	38, 821, 1164
BmrFI	CCNGG	4 cut(s)	591, 600, 695, 1719
BmrI	ACTGGG	2 cut(s)	522, 1486
BmsI	GCATC	7 cut(s)	171, 367, 967, 1215, 1345, 1674, 1726
BmuI	ACTGGG	2 cut(s)	522, 1486
BpiI	GAAGAC	1 cut(s)	266
BpmI	CTGGAG	1 cut(s)	81
Bpu10I	CCTNAGC	1 cut(s)	660
BpuEI	CTTGAG	3 cut(s)	1151, 1312, 1564
Bsa29I	ATCGAT	1 cut(s)	175
BsaBI	GATNNNNATC	1 cut(s)	645
BsaI	GGTCTC	1 cut(s)	762
BsaJI	CCNNGG	6 cut(s)	444, 492, 599, 679, 693, 1339
BsaXI	ACNNNNNCTCC	2 cut(s)	759, 789
Bsc4I	CCNNNNNNNGG	7 cut(s)	100, 450, 605, 835, 1082, 1478, 1700
Bse118I	RCCGGY	1 cut(s)	1095
Bse1I	ACTGG	4 cut(s)	517, 1000, 1072, 1481
Bse3DI	GCAATG	2 cut(s)	547, 1466
Bse8I	GATNNNNATC	1 cut(s)	645
BseBI	CCWGG	4 cut(s)	591, 600, 695, 1719
BseCI	ATCGAT	1 cut(s)	175
BseDI	CCNNGG	6 cut(s)	444, 492, 599, 679, 693, 1339
BseGI	GGATG	6 cut(s)	610, 810, 855, 859, 1233, 1624
BseJI	GATNNNNATC	1 cut(s)	645
BseLI	CCNNNNNNNGG	7 cut(s)	100, 450, 605, 835, 1082, 1478, 1700
BseMI	GCAATG	2 cut(s)	547, 1466
BseMII	CTCAG	2 cut(s)	36, 734
BseNI	ACTGG	4 cut(s)	517, 1000, 1072, 1481
BseRI	GAGGAG	1 cut(s)	968
BseSI	GKGCMC	2 cut(s)	520, 1651
BseXI	GCAGC	5 cut(s)	207, 260, 427, 436, 668
Bsh1285I	CGRYCG	2 cut(s)	175, 179
BshFI	GGCC	3 cut(s)	242, 458, 1717
BshNI	GGYRCC	1 cut(s)	819
BshVI	ATCGAT	1 cut(s)	175
BsiEI	CGRYCG	2 cut(s)	175, 179
BsiHKAI	GWGCWC	1 cut(s)	1651
BsiHKCI	CYCGRG	1 cut(s)	1693
BsiSI	CCGG	1 cut(s)	1096
BslFI	GGGAC	3 cut(s)	523, 585, 1126
BslI	CCNNNNNNNGG	7 cut(s)	100, 450, 605, 835, 1082, 1478, 1700
BsmAI	GTCTC	3 cut(s)	762, 868, 1221
BsmFI	GGGAC	3 cut(s)	523, 585, 1126
BsmI	GAATGC	1 cut(s)	297
BsnI	GGCC	3 cut(s)	242, 458, 1717
Bso31I	GGTCTC	1 cut(s)	762
BsoBI	CYCGRG	1 cut(s)	1693
Bsp1286I	GDGCHC	2 cut(s)	520, 1651
BspACI	CCGC	1 cut(s)	1312
BspANI	GGCC	3 cut(s)	242, 458, 1717
BspCNI	CTCAG	2 cut(s)	35, 735
BspDI	ATCGAT	1 cut(s)	175
BspHI	TCATGA	1 cut(s)	556
BspLI	GGNNCC	3 cut(s)	38, 821, 1164
BspPI	GGATC	7 cut(s)	31, 44, 723, 1157, 1170, 1559, 1751
BspT107I	GGYRCC	1 cut(s)	819
BspTI	CTTAAG	1 cut(s)	1613
BspTNI	GGTCTC	1 cut(s)	762
BsrDI	GCAATG	2 cut(s)	547, 1466
BsrFI	RCCGGY	1 cut(s)	1095
BsrI	ACTGG	4 cut(s)	517, 1000, 1072, 1481
BssAI	RCCGGY	1 cut(s)	1095
BssECI	CCNNGG	6 cut(s)	444, 492, 599, 679, 693, 1339
BssSI	CACGAG	1 cut(s)	797
BssT1I	CCWWGG	3 cut(s)	444, 679, 1339
Bst2BI	CACGAG	1 cut(s)	797
Bst2UI	CCWGG	4 cut(s)	591, 600, 695, 1719
Bst4CI	ACNGT	1 cut(s)	493
BstAFI	CTTAAG	1 cut(s)	1613
BstC8I	GCNNGC	5 cut(s)	193, 456, 830, 1153, 1346
BstDEI	CTNAG	5 cut(s)	22, 419, 660, 743, 1224
BstDSI	CCRYGG	1 cut(s)	492
BstF5I	GGATG	6 cut(s)	610, 810, 855, 859, 1233, 1624
BstH2I	RGCGCY	2 cut(s)	1277, 1335
BstHHI	GCGC	5 cut(s)	143, 1157, 1276, 1334, 1691
BstMAI	GTCTC	3 cut(s)	762, 868, 1221
BstMCI	CGRYCG	2 cut(s)	175, 179
BstMWI	GCNNNNNNNGC	6 cut(s)	248, 446, 455, 460, 546, 1695
BstNI	CCWGG	4 cut(s)	591, 600, 695, 1719
BstSCI	CCNGG	4 cut(s)	589, 598, 693, 1717
BstSFI	CTRYAG	3 cut(s)	234, 1629, 1711
BstSLI	GKGCMC	2 cut(s)	520, 1651
BstV1I	GCAGC	5 cut(s)	207, 260, 427, 436, 668
BstV2I	GAAGAC	1 cut(s)	266
BstX2I	RGATCY	4 cut(s)	36, 646, 1162, 1743
BstXI	CCANNNNNNTGG	1 cut(s)	480
BstYI	RGATCY	4 cut(s)	36, 646, 1162, 1743
Bsu15I	ATCGAT	1 cut(s)	175
BsuRI	GGCC	3 cut(s)	242, 458, 1717
BsuTUI	ATCGAT	1 cut(s)	175
BtgI	CCRYGG	1 cut(s)	492
BtsCI	GGATG	6 cut(s)	610, 810, 855, 859, 1233, 1624
BtsI	GCAGTG	1 cut(s)	1684
BtsIMutI	CAGTG	4 cut(s)	439, 993, 1474, 1684
Cac8I	GCNNGC	5 cut(s)	193, 456, 830, 1153, 1346
CciI	TCATGA	1 cut(s)	556
CfoI	GCGC	5 cut(s)	143, 1157, 1276, 1334, 1691
Cfr10I	RCCGGY	1 cut(s)	1095
Cfr13I	GGNCC	3 cut(s)	103, 596, 619
ClaI	ATCGAT	1 cut(s)	175
Csp6I	GTAC	5 cut(s)	352, 528, 820, 1522, 1749
CviAII	CATG	5 cut(s)	521, 557, 718, 1158, 1415
CviQI	GTAC	5 cut(s)	352, 528, 820, 1522, 1749
DdeI	CTNAG	5 cut(s)	22, 419, 660, 743, 1224
DraIII	CACNNNGTG	1 cut(s)	1152
EaeI	YGGCCR	1 cut(s)	240
Eco130I	CCWWGG	3 cut(s)	444, 679, 1339
Eco147I	AGGCCT	1 cut(s)	1717
Eco31I	GGTCTC	1 cut(s)	762
Eco32I	GATATC	2 cut(s)	1088, 1372
Eco47I	GGWCC	3 cut(s)	103, 596, 619
Eco47III	AGCGCT	2 cut(s)	1275, 1333
Eco57I	CTGAAG	1 cut(s)	402
Eco88I	CYCGRG	1 cut(s)	1693
EcoO109I	RGGNCCY	1 cut(s)	596
EcoRII	CCWGG	4 cut(s)	589, 598, 693, 1717
EcoRV	GATATC	2 cut(s)	1088, 1372
EcoT14I	CCWWGG	3 cut(s)	444, 679, 1339
ErhI	CCWWGG	3 cut(s)	444, 679, 1339
FaeI	CATG	5 cut(s)	524, 560, 721, 1161, 1418
FalI	AAGNNNNNCTT	2 cut(s)	1410, 1442
FaqI	GGGAC	3 cut(s)	523, 585, 1126
FatI	CATG	5 cut(s)	520, 556, 717, 1157, 1414
FbaI	TGATCA	1 cut(s)	336
Fnu4HI	GCNGC	5 cut(s)	196, 249, 425, 441, 657
FokI	GGATG	6 cut(s)	617, 797, 862, 866, 1220, 1631
Fsp4HI	GCNGC	5 cut(s)	196, 249, 425, 441, 657
FspAI	RTGCGCAY	1 cut(s)	1156
FspBI	CTAG	6 cut(s)	680, 825, 1121, 1407, 1584, 1652
FspI	TGCGCA	1 cut(s)	1156
GlaI	GCGC	5 cut(s)	142, 1156, 1275, 1333, 1690
GluI	GCNGC	5 cut(s)	196, 249, 425, 441, 657
GsuI	CTGGAG	1 cut(s)	81
HaeII	RGCGCY	2 cut(s)	1277, 1335
HaeIII	GGCC	3 cut(s)	242, 458, 1717
HapII	CCGG	1 cut(s)	1096
HhaI	GCGC	5 cut(s)	143, 1157, 1276, 1334, 1691
Hin1II	CATG	5 cut(s)	524, 560, 721, 1161, 1418
Hin6I	GCGC	5 cut(s)	141, 1155, 1274, 1332, 1689
HinP1I	GCGC	5 cut(s)	141, 1155, 1274, 1332, 1689
HpaII	CCGG	1 cut(s)	1096
HphI	GGTGA	2 cut(s)	1190, 1636
Hpy166II	GTNNAC	2 cut(s)	1561, 1649
Hpy188I	TCNGA	9 cut(s)	47, 214, 623, 744, 760, 849, 944, 1059, 1491
Hpy188III	TCNNGA	7 cut(s)	98, 557, 879, 962, 1130, 1140, 1543
Hpy8I	GTNNAC	2 cut(s)	1561, 1649
HpyAV	CCTTC	8 cut(s)	50, 219, 265, 618, 930, 1412, 1634, 1649
HpyCH4III	ACNGT	1 cut(s)	493
HpyCH4IV	ACGT	2 cut(s)	530, 1199
HpyF10VI	GCNNNNNNNGC	6 cut(s)	248, 446, 455, 460, 546, 1695
HpyF3I	CTNAG	5 cut(s)	22, 419, 660, 743, 1224
HpySE526I	ACGT	2 cut(s)	530, 1199
Hsp92II	CATG	5 cut(s)	524, 560, 721, 1161, 1418
HspAI	GCGC	5 cut(s)	141, 1155, 1274, 1332, 1689
KpnI	GGTACC	1 cut(s)	823
Ksp22I	TGATCA	1 cut(s)	336
LmnI	GCTCC	1 cut(s)	1463
Lsp1109I	GCAGC	5 cut(s)	207, 260, 427, 436, 668
LweI	GCATC	7 cut(s)	171, 367, 967, 1215, 1345, 1674, 1726
MaeI	CTAG	6 cut(s)	680, 825, 1121, 1407, 1584, 1652
MaeII	ACGT	2 cut(s)	530, 1199
MaeIII	GTNAC	6 cut(s)	284, 412, 697, 1009, 1318, 1624
MboII	GAAGA	7 cut(s)	157, 233, 268, 271, 338, 1442, 1720
MfeI	CAATTG	1 cut(s)	579
MflI	RGATCY	4 cut(s)	36, 646, 1162, 1743
MhlI	GDGCHC	2 cut(s)	520, 1651
MlsI	TGGCCA	1 cut(s)	242
MluCI	AATT	5 cut(s)	51, 228, 579, 687, 706
MluNI	TGGCCA	1 cut(s)	242
MlyI	GAGTC	6 cut(s)	409, 595, 684, 870, 1396, 1404
Mox20I	TGGCCA	1 cut(s)	242
MscI	TGGCCA	1 cut(s)	242
MseI	TTAA	9 cut(s)	27, 50, 488, 651, 723, 1026, 1043, 1455, 1614
MslI	CAYNNNNRTG	2 cut(s)	478, 561
Msp20I	TGGCCA	1 cut(s)	242
MspCI	CTTAAG	1 cut(s)	1613
MspI	CCGG	1 cut(s)	1096
MspR9I	CCNGG	4 cut(s)	591, 600, 695, 1719
MunI	CAATTG	1 cut(s)	579
Mva1269I	GAATGC	1 cut(s)	297
MvaI	CCWGG	4 cut(s)	591, 600, 695, 1719
MwoI	GCNNNNNNNGC	6 cut(s)	248, 446, 455, 460, 546, 1695
NlaIII	CATG	5 cut(s)	524, 560, 721, 1161, 1418
NlaIV	GGNNCC	3 cut(s)	38, 821, 1164
NmuCI	GTSAC	2 cut(s)	412, 1624
NsbI	TGCGCA	1 cut(s)	1156
PagI	TCATGA	1 cut(s)	556
PceI	AGGCCT	1 cut(s)	1717
PctI	GAATGC	1 cut(s)	297
PfeI	GAWTC	7 cut(s)	553, 755, 958, 965, 1136, 1143, 1302
PfoI	TCCNGGA	1 cut(s)	589
PkrI	GCNGC	5 cut(s)	197, 250, 426, 442, 658
Ple19I	CGATCG	2 cut(s)	175, 179
PleI	GAGTC	6 cut(s)	409, 594, 683, 869, 1396, 1404
PpsI	GAGTC	6 cut(s)	409, 594, 683, 869, 1396, 1404
PpuMI	RGGWCCY	1 cut(s)	596
PshBI	ATTAAT	1 cut(s)	50
Psp1406I	AACGTT	1 cut(s)	1199
Psp5II	RGGWCCY	1 cut(s)	596
Psp6I	CCWGG	4 cut(s)	589, 598, 693, 1717
PspGI	CCWGG	4 cut(s)	589, 598, 693, 1717
PspN4I	GGNNCC	3 cut(s)	38, 821, 1164
PspPI	GGNCC	3 cut(s)	103, 596, 619
PspPPI	RGGWCCY	1 cut(s)	596
PsuI	RGATCY	4 cut(s)	36, 646, 1162, 1743
PvuI	CGATCG	2 cut(s)	175, 179
RsaI	GTAC	5 cut(s)	353, 529, 821, 1523, 1750
RsaNI	GTAC	5 cut(s)	352, 528, 820, 1522, 1749
RseI	CAYNNNNRTG	2 cut(s)	478, 561
SaqAI	TTAA	9 cut(s)	27, 50, 488, 651, 723, 1026, 1043, 1455, 1614
SatI	GCNGC	5 cut(s)	196, 249, 425, 441, 657
Sau96I	GGNCC	3 cut(s)	103, 596, 619
SchI	GAGTC	6 cut(s)	409, 595, 684, 870, 1396, 1404
ScrFI	CCNGG	4 cut(s)	591, 600, 695, 1719
SduI	GDGCHC	2 cut(s)	520, 1651
SfaNI	GCATC	7 cut(s)	171, 367, 967, 1215, 1345, 1674, 1726
SfcI	CTRYAG	3 cut(s)	234, 1629, 1711
SinI	GGWCC	3 cut(s)	103, 596, 619
SmiMI	CAYNNNNRTG	2 cut(s)	478, 561
SmlI	CTYRAG	4 cut(s)	1130, 1327, 1543, 1613
SmoI	CTYRAG	4 cut(s)	1130, 1327, 1543, 1613
Sse9I	AATT	5 cut(s)	51, 228, 579, 687, 706
SseBI	AGGCCT	1 cut(s)	1717
SsiI	CCGC	1 cut(s)	1312
SspMI	CTAG	6 cut(s)	680, 825, 1121, 1407, 1584, 1652
StuI	AGGCCT	1 cut(s)	1717
StyD4I	CCNGG	4 cut(s)	589, 598, 693, 1717
StyI	CCWWGG	3 cut(s)	444, 679, 1339
TaaI	ACNGT	1 cut(s)	493
TaiI	ACGT	2 cut(s)	533, 1202
TaqI	TCGA	8 cut(s)	175, 187, 551, 859, 910, 1362, 1368, 1384
TasI	AATT	5 cut(s)	51, 228, 579, 687, 706
TatI	WGTACW	1 cut(s)	1521
TfiI	GAWTC	7 cut(s)	553, 755, 958, 965, 1136, 1143, 1302
Tru1I	TTAA	9 cut(s)	27, 50, 488, 651, 723, 1026, 1043, 1455, 1614
Tru9I	TTAA	9 cut(s)	27, 50, 488, 651, 723, 1026, 1043, 1455, 1614
TscAI	CASTG	4 cut(s)	439, 1000, 1481, 1691
TseFI	GTSAC	2 cut(s)	412, 1624
TseI	GCWGC	5 cut(s)	195, 248, 424, 440, 656
Tsp45I	GTSAC	2 cut(s)	412, 1624
TspDTI	ATGAA	9 cut(s)	52, 108, 284, 545, 889, 1178, 1222, 1431, 1529
TspRI	CASTG	4 cut(s)	439, 1000, 1481, 1691
Vha464I	CTTAAG	1 cut(s)	1613
VneI	GTGCAC	1 cut(s)	1647
VpaK11BI	GGWCC	3 cut(s)	103, 596, 619
VspI	ATTAAT	1 cut(s)	50
XapI	RAATTY	1 cut(s)	706
XcmI	CCANNNNNNNNNTGG	2 cut(s)	271, 451
XmaJI	CCTAGG	1 cut(s)	679
XspI	CTAG	6 cut(s)	680, 825, 1121, 1407, 1584, 1652

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.