Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0002464

Physical Location & Seq

Genomic Coordinates Reverse (-)

53118874 .. 53122704

Gene Structure 3831 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G026110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGGGTATGTTGGGGCTTGTTATCCGAGTAAACTGTCTATTCTTGTTATTGTTCTTGGTATGTTCTACCCCTCCGGAGGACCACATCAAGTGTTCATCGTCAAACAACACAAACTGCACCATCACAAACTCCTACGGCATCTTCCCCGATCGAACTGTCTGTAAAGCGACTAGTGTATCATACCCTAGTACGGAGGAAGAACTCGTTAAGGTAGTGGCTGATGCAACCAAGAACAAGAAGAGCATGAAGGTGGCGACTCGTTACTCCCACAGCATTCCCAAGCTGGTTTGCCCGCGCGGCGAAGATGGGCTGATCATAAGCACCAACAAACCTCAACCCGGCGTGACGCTTAGGCAGGTGATCAGTGAGGCTGCCAAAGTTGGCTTGGCGTTGCCTTACGCACCATATTGGTGGGGGCTAACTGTCGGTGGGTTGCTAGGGACTGGTGCGCATGGAAGCACATTATGGGCTAAGGGAAGCTCTGTTCATGATTATGTGGTGGGACTTAGGATTGTGAGTCCAGGAGGGCCCGAAGATGGTTATGTCAAGGTGCGAAGTCTCAGTCAAGGTGATAAAGATCTTAATGCAGCTAAGGTTTCACTTGGAGTCTTAGGAGTAATATCACAGGTTACTCTAAACCTACAACCCATGTTCAAGAGGTCCATCACATATTTGAGCAAGAACGATTCTGACTTGGGAGATCAAGTGCTTAAGTTTGGCAAGCAGCATGAGTTTGCAGACATATTATGGTATCCTAGCCAGCACAAGGCAGTTTATCGAGTAGATGATCGTATCTCCTCAAACACATCCGGAAATGGTTTTTATGATTTCCTTCCTTTTCGAGCAACACCTTCACTTGCACTGGCAGTTATAAGAACGGCAGAAGAGAACCAAGAATCAAAAAGCGATGCTGATGGGAAATGCTCTGGGGCAAAGTTGGTTACATCTGGGCTTCTATCCAATGCTTATGGTTTGACAAACAATGGTATAATCTTTACTGGTTATCCTGTGATTGGATATCAGAACCGGCTTCAAGCATCTGGAACTTGCCTTGATAGTCTTCAAGATTCAAGAATCACGGCATGCGCATGGGACCCAAGGATCAAGGGCGAGTTTTTTCATCAAACAACCTTCAGCATCAGCTTGTCCGTGGCCAAAAGCTTCATTCAAGACGTGCAGAAGCTAGTTGAGATTGAACCCAAATCCCTCTGTGGAGTAGAAATCTACAATGGAATCCTAATGCGCTATGTCACCGCATCAACTGCTTACCTTGGCAAACAAGTAGATGCAATTGACTTCGACATCACTTATTACCGAAGCAAGGACCCCATGACGCCTAGGCTCCATGAAGGCATACTTGAAGAAATAGAGCAATTGGCACTGTTTAAGTACGGAGCATTGCCACATTGGGGAAAGAATAGGAACATAGCCTTTGATGGGGTGATGAAGAAGTACAAAAATGGTAAGGAGTTCTTGAGGGCTAAGGAAGTGTATGACCCAATGGGGCTATTCTCTAGTGAATGGAGTGACCAGGTCCTTGGAATTAAGGGTGAGGTTAACTTAGTCAAGGAAGGGTGCGCCCTAGAAGGGTTGTGCATTTGCTCACAAGACATTCATTGTGCCCCAAGAAAGGGCTACTTCTGTAGACCGGGGAAAGTATTTAGGGATGCGAGGATCTGTACTCCAATTCATAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005618 GO:0005623 GO:0005975 GO:0005996 GO:0006082 GO:0006732 GO:0006766 GO:0006767 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016051 GO:0016053 GO:0016491 GO:0016614 GO:0016899 GO:0019752 GO:0019852 GO:0019853 GO:0030312 GO:0036094 GO:0042364 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044464 GO:0046364 GO:0046394 GO:0048037 GO:0050105 GO:0050660 GO:0050662 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:0071944 GO:0097159 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ALO FAD_binding_4

Protein Analysis

565

Amino Acids

62.1

Weight (kDa)

8.61

Isoelectric Point (pI)

31.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	59 - 176	2.3e-07	FAD binding domain
ALO	PF04030	373 - 511	3.8e-09	D-arabinono-1,4-lactone oxidase
GULLO2-like_3rd	PF22906	523 - 561	1e-23	L-gulonolactone oxidase 2-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46750 AT2G46760 AT5G56470
fragaria_vesca	FvH4_7g17510 FvH4_7g17511
malus_domestica	MD01G1087800.v1.1 MD07G1157900.v1.1
prunus_persica	Prupe.2G196400_v2.0.a1
pyrus_communis	pycom01g11170 pycom07g15450
rosa_chinensis	RchiOBHm_Chr1g0360921 RchiOBHm_Chr1g0360951 RchiOBHm_Chr3g0481291 RchiOBHm_Chr5g0029651
rosa_laevigata	RLG00000019622 RLG00000027767 RLG00000027769
rosa_multiflora	Rmu_sc0000206.1_g000019 Rmu_sc0000677.1_g000024 Rmu_sc0002207.1_g000003 Rmu_sc0008012.1_g000002
rosa_roxburghii	Rroxscaffold_3G00228090 Rroxscaffold_4G00295680 Rroxscaffold_4G00295700 Rroxscaffold_5G00385060
rosa_rugosa	Rorug01G0283500 Rorug01G0283800 Rorug03G0191000
rosa_samantha	Rh1AG295000 Rh1BG259800 Rh1BG259900 Rh1CG277200 Rh1DG289900 Rh2BG393900 Rh3AG241900 Rh3BG277100 Rh3DG268900
rosa_wichuraiana	Rw1G026070 Rw1G026100 Rw1G026110 Rw3G021610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	872
AarI	CACCTGC	1 cut(s)	346
Acc16I	TGCGCA	2 cut(s)	450, 1087
Acc36I	ACCTGC	1 cut(s)	346
AccI	GTMKAC	1 cut(s)	1645
AccII	CGCG	2 cut(s)	295, 297
AccIII	TCCGGA	2 cut(s)	73, 809
AciI	CCGC	3 cut(s)	293, 297, 1254
AclWI	GGATC	2 cut(s)	1109, 1682
AcoI	YGGCCR	1 cut(s)	1152
AcuI	CTGAAG	1 cut(s)	1117
AcyI	GRCGYC	1 cut(s)	1334
AfaI	GTAC	4 cut(s)	190, 1391, 1454, 1681
AflII	CTTAAG	1 cut(s)	710
AgsI	TTSAA	7 cut(s)	655, 1034, 1064, 1071, 1169, 1196, 1361
AhlI	ACTAGT	1 cut(s)	170
AjiI	CACGTC	1 cut(s)	1174
AjnI	CCWGG	2 cut(s)	520, 1530
AloI	GAACNNNNNNTCC	2 cut(s)	468, 500
AluBI	AGCT	6 cut(s)	283, 480, 590, 1143, 1161, 1183
AluI	AGCT	6 cut(s)	283, 480, 590, 1143, 1161, 1183
Alw26I	GTCTC	1 cut(s)	563
AlwI	GGATC	2 cut(s)	1109, 1682
Aor13HI	TCCGGA	2 cut(s)	73, 809
AoxI	GGCC	2 cut(s)	527, 1152
ApaI	GGGCCC	1 cut(s)	531
ApeKI	GCWGC	3 cut(s)	371, 587, 724
ArsI	GACNNNNNNTTYG	2 cut(s)	547, 579
AspA2I	CCTAGG	1 cut(s)	1337
AspLEI	GCGC	5 cut(s)	297, 451, 1088, 1245, 1580
AspS9I	GGNCC	7 cut(s)	79, 527, 528, 660, 1093, 1324, 1534
AsuC2I	CCSGG	2 cut(s)	339, 1650
AsuHPI	GGTGA	5 cut(s)	370, 581, 1243, 1453, 1562
AvaII	GGWCC	5 cut(s)	79, 660, 1093, 1324, 1534
AvrII	CCTAGG	1 cut(s)	1337
BaeGI	GKGCMC	2 cut(s)	531, 1624
BaeI	ACNNNNGTAYC	2 cut(s)	159, 192
BalI	TGGCCA	1 cut(s)	1154
BanII	GRGCYC	1 cut(s)	531
BbsI	GAAGAC	1 cut(s)	1052
BbvI	GCAGC	3 cut(s)	358, 599, 736
BccI	CCATC	6 cut(s)	128, 299, 530, 671, 908, 1430
BceAI	ACGGC	3 cut(s)	151, 894, 1095
BciT130I	CCWGG	2 cut(s)	522, 1532
BciVI	GTATCC	1 cut(s)	762
BclI	TGATCA	2 cut(s)	312, 360
BcnI	CCSGG	2 cut(s)	339, 1650
BcoDI	GTCTC	1 cut(s)	563
BcuI	ACTAGT	1 cut(s)	170
BfaI	CTAG	8 cut(s)	171, 186, 437, 756, 1184, 1338, 1515, 1583
BfmI	CTRYAG	1 cut(s)	1642
BfrI	CTTAAG	1 cut(s)	710
BfuAI	ACCTGC	1 cut(s)	346
BfuI	GTATCC	1 cut(s)	762
BglI	GCCNNNNNGGC	1 cut(s)	297
BglII	AGATCT	1 cut(s)	577
BisI	GCNGC	4 cut(s)	298, 372, 588, 725
BlnI	CCTAGG	1 cut(s)	1337
BlsI	GCNGC	4 cut(s)	299, 373, 589, 726
Bme1390I	CCNGG	4 cut(s)	339, 522, 1532, 1650
Bme18I	GGWCC	5 cut(s)	79, 660, 1093, 1324, 1534
BmgBI	CACGTC	1 cut(s)	1174
BmgT120I	GGNCC	7 cut(s)	79, 527, 528, 660, 1093, 1324, 1534
BmiI	GGNNCC	5 cut(s)	529, 1094, 1095, 1326, 1343
BmrFI	CCNGG	4 cut(s)	339, 522, 1532, 1650
BmsI	GCATC	8 cut(s)	147, 211, 898, 1046, 1146, 1265, 1276, 1657
BpiI	GAAGAC	1 cut(s)	1052
Bpu10I	CCTNAGC	4 cut(s)	350, 471, 591, 1482
BpuEI	CTTGAG	1 cut(s)	1495
BpuMI	CCSGG	2 cut(s)	339, 1650
BsaBI	GATNNNNATC	1 cut(s)	576
BsaHI	GRCGYC	1 cut(s)	1334
BsaJI	CCNNGG	6 cut(s)	1097, 1149, 1270, 1337, 1537, 1649
BsaWI	WCCGGW	2 cut(s)	73, 809
BsaXI	ACNNNNNCTCC	2 cut(s)	690, 720
Bse118I	RCCGGY	1 cut(s)	1026
Bse1I	ACTGG	3 cut(s)	448, 867, 1003
Bse3DI	GCAATG	1 cut(s)	1397
Bse8I	GATNNNNATC	1 cut(s)	576
BseAI	TCCGGA	2 cut(s)	73, 809
BseBI	CCWGG	2 cut(s)	522, 1532
BseDI	CCNNGG	6 cut(s)	1097, 1149, 1270, 1337, 1537, 1649
BseGI	GGATG	2 cut(s)	806, 1672
BseJI	GATNNNNATC	1 cut(s)	576
BseMI	GCAATG	1 cut(s)	1397
BseMII	CTCAG	1 cut(s)	574
BseNI	ACTGG	3 cut(s)	448, 867, 1003
BseRI	GAGGAG	1 cut(s)	787
BseSI	GKGCMC	2 cut(s)	531, 1624
BseXI	GCAGC	3 cut(s)	358, 599, 736
BsgI	GTGCAG	2 cut(s)	100, 1196
Bsh1236I	CGCG	2 cut(s)	295, 297
Bsh1285I	CGRYCG	1 cut(s)	151
BshFI	GGCC	2 cut(s)	529, 1154
BsiEI	CGRYCG	1 cut(s)	151
BsiSI	CCGG	5 cut(s)	74, 339, 810, 1027, 1649
BslFI	GGGAC	3 cut(s)	454, 516, 1106
BsmAI	GTCTC	1 cut(s)	563
BsmFI	GGGAC	3 cut(s)	454, 516, 1106
BsmI	GAATGC	1 cut(s)	273
BsnI	GGCC	2 cut(s)	529, 1154
Bsp120I	GGGCCC	1 cut(s)	527
Bsp1286I	GDGCHC	2 cut(s)	531, 1624
Bsp13I	TCCGGA	2 cut(s)	73, 809
Bsp143I	GATC	8 cut(s)	148, 312, 360, 577, 700, 787, 1101, 1674
BspACI	CCGC	3 cut(s)	293, 297, 1254
BspANI	GGCC	2 cut(s)	529, 1154
BspCNI	CTCAG	1 cut(s)	573
BspEI	TCCGGA	2 cut(s)	73, 809
BspFNI	CGCG	2 cut(s)	295, 297
BspHI	TCATGA	1 cut(s)	487
BspLI	GGNNCC	5 cut(s)	529, 1094, 1095, 1326, 1343
BspMI	ACCTGC	1 cut(s)	346
BspPI	GGATC	2 cut(s)	1109, 1682
BspQI	GCTCTTC	1 cut(s)	233
BspTI	CTTAAG	1 cut(s)	710
BsrDI	GCAATG	1 cut(s)	1397
BsrFI	RCCGGY	1 cut(s)	1026
BsrI	ACTGG	3 cut(s)	448, 867, 1003
BssAI	RCCGGY	1 cut(s)	1026
BssECI	CCNNGG	6 cut(s)	1097, 1149, 1270, 1337, 1537, 1649
BssMI	GATC	8 cut(s)	148, 312, 360, 577, 700, 787, 1101, 1674
BssNI	GRCGYC	1 cut(s)	1334
BssT1I	CCWWGG	4 cut(s)	1097, 1270, 1337, 1537
Bst2UI	CCWGG	2 cut(s)	522, 1532
Bst4CI	ACNGT	4 cut(s)	35, 157, 424, 1383
Bst6I	CTCTTC	2 cut(s)	233, 879
BstACI	GRCGYC	1 cut(s)	1334
BstAFI	CTTAAG	1 cut(s)	710
BstAPI	GCANNNNNTGC	1 cut(s)	1262
BstC8I	GCNNGC	4 cut(s)	293, 722, 761, 1084
BstDEI	CTNAG	8 cut(s)	350, 471, 506, 560, 591, 610, 1482, 1561
BstDSI	CCRYGG	1 cut(s)	1149
BstF5I	GGATG	2 cut(s)	806, 1672
BstFNI	CGCG	2 cut(s)	295, 297
BstHHI	GCGC	5 cut(s)	297, 451, 1088, 1245, 1580
BstKTI	GATC	8 cut(s)	151, 315, 363, 580, 703, 790, 1104, 1677
BstMAI	GTCTC	1 cut(s)	563
BstMBI	GATC	8 cut(s)	148, 312, 360, 577, 700, 787, 1101, 1674
BstMCI	CGRYCG	1 cut(s)	151
BstMWI	GCNNNNNNNGC	2 cut(s)	297, 1262
BstNI	CCWGG	2 cut(s)	522, 1532
BstNSI	RCATGY	1 cut(s)	1086
BstSCI	CCNGG	4 cut(s)	337, 520, 1530, 1648
BstSFI	CTRYAG	1 cut(s)	1642
BstSLI	GKGCMC	2 cut(s)	531, 1624
BstUI	CGCG	2 cut(s)	295, 297
BstV1I	GCAGC	3 cut(s)	358, 599, 736
BstV2I	GAAGAC	1 cut(s)	1052
BstX2I	RGATCY	2 cut(s)	577, 1674
BstXI	CCANNNNNNTGG	2 cut(s)	411, 1538
BstYI	RGATCY	2 cut(s)	577, 1674
BsuI	GTATCC	1 cut(s)	762
BsuRI	GGCC	2 cut(s)	529, 1154
BtgI	CCRYGG	1 cut(s)	1149
BtgZI	GCGATG	1 cut(s)	921
BtrI	CACGTC	1 cut(s)	1174
BtsCI	GGATG	2 cut(s)	806, 1672
BtsIMutI	CAGTG	3 cut(s)	370, 860, 1379
BveI	ACCTGC	1 cut(s)	346
Cac8I	GCNNGC	4 cut(s)	293, 722, 761, 1084
CciI	TCATGA	1 cut(s)	487
CfoI	GCGC	5 cut(s)	297, 451, 1088, 1245, 1580
Cfr10I	RCCGGY	1 cut(s)	1026
Cfr13I	GGNCC	7 cut(s)	79, 527, 528, 660, 1093, 1324, 1534
CseI	GACGC	2 cut(s)	355, 1342
CsiI	ACCWGGT	1 cut(s)	1530
Csp6I	GTAC	4 cut(s)	189, 1390, 1453, 1680
CviAII	CATG	9 cut(s)	244, 452, 488, 649, 728, 1083, 1089, 1330, 1346
CviQI	GTAC	4 cut(s)	189, 1390, 1453, 1680
DdeI	CTNAG	8 cut(s)	350, 471, 506, 560, 591, 610, 1482, 1561
DpnI	GATC	8 cut(s)	150, 314, 362, 579, 702, 789, 1103, 1676
DpnII	GATC	8 cut(s)	148, 312, 360, 577, 700, 787, 1101, 1674
EaeI	YGGCCR	1 cut(s)	1152
Eam1104I	CTCTTC	2 cut(s)	233, 879
EarI	CTCTTC	2 cut(s)	233, 879
Eco130I	CCWWGG	4 cut(s)	1097, 1270, 1337, 1537
Eco24I	GRGCYC	1 cut(s)	531
Eco32I	GATATC	1 cut(s)	1019
Eco47I	GGWCC	5 cut(s)	79, 660, 1093, 1324, 1534
Eco57I	CTGAAG	1 cut(s)	1117
EcoO109I	RGGNCCY	4 cut(s)	527, 1093, 1324, 1534
EcoRII	CCWGG	2 cut(s)	520, 1530
EcoRV	GATATC	1 cut(s)	1019
EcoT14I	CCWWGG	4 cut(s)	1097, 1270, 1337, 1537
EcoT38I	GRGCYC	1 cut(s)	531
ErhI	CCWWGG	4 cut(s)	1097, 1270, 1337, 1537
FaeI	CATG	9 cut(s)	247, 455, 491, 652, 731, 1086, 1092, 1333, 1349
FalI	AAGNNNNNCTT	6 cut(s)	1341, 1373, 1457, 1489, 1622, 1654
FaqI	GGGAC	3 cut(s)	454, 516, 1106
FatI	CATG	9 cut(s)	243, 451, 487, 648, 727, 1082, 1088, 1329, 1345
FauI	CCCGC	1 cut(s)	300
FbaI	TGATCA	2 cut(s)	312, 360
FblI	GTMKAC	1 cut(s)	1645
Fnu4HI	GCNGC	4 cut(s)	298, 372, 588, 725
FokI	GGATG	2 cut(s)	793, 1679
FriOI	GRGCYC	1 cut(s)	531
Fsp4HI	GCNGC	4 cut(s)	298, 372, 588, 725
FspAI	RTGCGCAY	2 cut(s)	450, 1087
FspBI	CTAG	8 cut(s)	171, 186, 437, 756, 1184, 1338, 1515, 1583
FspI	TGCGCA	2 cut(s)	450, 1087
GlaI	GCGC	5 cut(s)	296, 450, 1087, 1244, 1579
GluI	GCNGC	4 cut(s)	298, 372, 588, 725
HaeIII	GGCC	2 cut(s)	529, 1154
HapII	CCGG	5 cut(s)	74, 339, 810, 1027, 1649
HgaI	GACGC	2 cut(s)	355, 1342
HhaI	GCGC	5 cut(s)	297, 451, 1088, 1245, 1580
Hin1I	GRCGYC	1 cut(s)	1334
Hin1II	CATG	9 cut(s)	247, 455, 491, 652, 731, 1086, 1092, 1333, 1349
Hin6I	GCGC	5 cut(s)	295, 449, 1086, 1243, 1578
HinP1I	GCGC	5 cut(s)	295, 449, 1086, 1243, 1578
HincII	GTYRAC	1 cut(s)	1558
HindII	GTYRAC	1 cut(s)	1558
HindIII	AAGCTT	1 cut(s)	1159
HinfI	GANTC	8 cut(s)	256, 517, 606, 686, 896, 1067, 1074, 1233
HpaI	GTTAAC	1 cut(s)	1558
HpaII	CCGG	5 cut(s)	74, 339, 810, 1027, 1649
HphI	GGTGA	5 cut(s)	370, 581, 1243, 1453, 1562
Hpy166II	GTNNAC	3 cut(s)	31, 1558, 1646
Hpy188I	TCNGA	3 cut(s)	26, 691, 1023
Hpy188III	TCNNGA	9 cut(s)	74, 488, 655, 810, 1041, 1064, 1071, 1169, 1474
Hpy8I	GTNNAC	3 cut(s)	31, 1558, 1646
HpyAV	CCTTC	7 cut(s)	241, 842, 861, 1141, 1343, 1565, 1580
HpyCH4III	ACNGT	4 cut(s)	35, 157, 424, 1383
HpyCH4IV	ACGT	1 cut(s)	1173
HpyCH4V	TGCA	8 cut(s)	117, 224, 587, 737, 860, 1177, 1289, 1596
HpyF10VI	GCNNNNNNNGC	2 cut(s)	297, 1262
HpyF3I	CTNAG	8 cut(s)	350, 471, 506, 560, 591, 610, 1482, 1561
HpySE526I	ACGT	1 cut(s)	1173
Hsp92I	GRCGYC	1 cut(s)	1334
Hsp92II	CATG	9 cut(s)	247, 455, 491, 652, 731, 1086, 1092, 1333, 1349
HspAI	GCGC	5 cut(s)	295, 449, 1086, 1243, 1578
KflI	GGGWCCC	1 cut(s)	1093
Kpn2I	TCCGGA	2 cut(s)	73, 809
Ksp22I	TGATCA	2 cut(s)	312, 360
KspAI	GTTAAC	1 cut(s)	1558
Kzo9I	GATC	8 cut(s)	148, 312, 360, 577, 700, 787, 1101, 1674
LguI	GCTCTTC	1 cut(s)	233
LmnI	GCTCC	2 cut(s)	1347, 1394
Lsp1109I	GCAGC	3 cut(s)	358, 599, 736
LweI	GCATC	8 cut(s)	147, 211, 898, 1046, 1146, 1265, 1276, 1657
MabI	ACCWGGT	1 cut(s)	1530
MaeI	CTAG	8 cut(s)	171, 186, 437, 756, 1184, 1338, 1515, 1583
MaeII	ACGT	1 cut(s)	1173
MaeIII	GTNAC	6 cut(s)	260, 343, 628, 940, 1249, 1526
MalI	GATC	8 cut(s)	150, 314, 362, 579, 702, 789, 1103, 1676
MboI	GATC	8 cut(s)	148, 312, 360, 577, 700, 787, 1101, 1674
MboII	GAAGA	9 cut(s)	133, 209, 250, 314, 545, 896, 1052, 1373, 1459
MfeI	CAATTG	2 cut(s)	1290, 1373
MflI	RGATCY	2 cut(s)	577, 1674
MhlI	GDGCHC	2 cut(s)	531, 1624
MlsI	TGGCCA	1 cut(s)	1154
MluCI	AATT	5 cut(s)	1290, 1373, 1542, 1686, 1693
MluNI	TGGCCA	1 cut(s)	1154
MlyI	GAGTC	3 cut(s)	250, 526, 615
Mox20I	TGGCCA	1 cut(s)	1154
MroI	TCCGGA	2 cut(s)	73, 809
MscI	TGGCCA	1 cut(s)	1154
MseI	TTAA	6 cut(s)	207, 582, 711, 1386, 1545, 1557
MslI	CAYNNNNRTG	4 cut(s)	248, 409, 492, 1087
Msp20I	TGGCCA	1 cut(s)	1154
MspCI	CTTAAG	1 cut(s)	710
MspI	CCGG	5 cut(s)	74, 339, 810, 1027, 1649
MspR9I	CCNGG	4 cut(s)	339, 522, 1532, 1650
MunI	CAATTG	2 cut(s)	1290, 1373
Mva1269I	GAATGC	1 cut(s)	273
MvaI	CCWGG	2 cut(s)	522, 1532
MvnI	CGCG	2 cut(s)	295, 297
MwoI	GCNNNNNNNGC	2 cut(s)	297, 1262
NciI	CCSGG	2 cut(s)	339, 1650
NdeII	GATC	8 cut(s)	148, 312, 360, 577, 700, 787, 1101, 1674
NlaIII	CATG	9 cut(s)	247, 455, 491, 652, 731, 1086, 1092, 1333, 1349
NlaIV	GGNNCC	5 cut(s)	529, 1094, 1095, 1326, 1343
NmuCI	GTSAC	3 cut(s)	343, 1249, 1526
NsbI	TGCGCA	2 cut(s)	450, 1087
NspI	RCATGY	1 cut(s)	1086
PaeI	GCATGC	1 cut(s)	1086
PagI	TCATGA	1 cut(s)	487
PaqCI	CACCTGC	1 cut(s)	346
PciSI	GCTCTTC	1 cut(s)	233
PctI	GAATGC	1 cut(s)	273
PfeI	GAWTC	5 cut(s)	686, 896, 1067, 1074, 1233
PflFI	GACNNNGTC	1 cut(s)	1532
PfoI	TCCNGGA	1 cut(s)	520
PkrI	GCNGC	4 cut(s)	299, 373, 589, 726
Ple19I	CGATCG	1 cut(s)	151
PleI	GAGTC	3 cut(s)	250, 525, 614
PpsI	GAGTC	3 cut(s)	250, 525, 614
PpuMI	RGGWCCY	3 cut(s)	1093, 1324, 1534
PsiI	TTATAA	1 cut(s)	872
Psp5II	RGGWCCY	3 cut(s)	1093, 1324, 1534
Psp6I	CCWGG	2 cut(s)	520, 1530
PspGI	CCWGG	2 cut(s)	520, 1530
PspN4I	GGNNCC	5 cut(s)	529, 1094, 1095, 1326, 1343
PspOMI	GGGCCC	1 cut(s)	527
PspPI	GGNCC	7 cut(s)	79, 527, 528, 660, 1093, 1324, 1534
PspPPI	RGGWCCY	3 cut(s)	1093, 1324, 1534
PsuI	RGATCY	2 cut(s)	577, 1674
PsyI	GACNNNGTC	1 cut(s)	1532
PvuI	CGATCG	1 cut(s)	151
RsaI	GTAC	4 cut(s)	190, 1391, 1454, 1681
RsaNI	GTAC	4 cut(s)	189, 1390, 1453, 1680
RseI	CAYNNNNRTG	4 cut(s)	248, 409, 492, 1087
SapI	GCTCTTC	1 cut(s)	233
SaqAI	TTAA	6 cut(s)	207, 582, 711, 1386, 1545, 1557
SatI	GCNGC	4 cut(s)	298, 372, 588, 725
Sau3AI	GATC	8 cut(s)	148, 312, 360, 577, 700, 787, 1101, 1674
Sau96I	GGNCC	7 cut(s)	79, 527, 528, 660, 1093, 1324, 1534
SchI	GAGTC	3 cut(s)	250, 526, 615
ScrFI	CCNGG	4 cut(s)	339, 522, 1532, 1650
SduI	GDGCHC	2 cut(s)	531, 1624
SexAI	ACCWGGT	1 cut(s)	1530
SfaNI	GCATC	8 cut(s)	147, 211, 898, 1046, 1146, 1265, 1276, 1657
SfcI	CTRYAG	1 cut(s)	1642
SinI	GGWCC	5 cut(s)	79, 660, 1093, 1324, 1534
SmiMI	CAYNNNNRTG	4 cut(s)	248, 409, 492, 1087
SmlI	CTYRAG	2 cut(s)	710, 1474
SmoI	CTYRAG	2 cut(s)	710, 1474
SpeI	ACTAGT	1 cut(s)	170
SphI	GCATGC	1 cut(s)	1086
Sse9I	AATT	5 cut(s)	1290, 1373, 1542, 1686, 1693
SsiI	CCGC	3 cut(s)	293, 297, 1254
SspMI	CTAG	8 cut(s)	171, 186, 437, 756, 1184, 1338, 1515, 1583
StyD4I	CCNGG	4 cut(s)	337, 520, 1530, 1648
StyI	CCWWGG	4 cut(s)	1097, 1270, 1337, 1537
TaaI	ACNGT	4 cut(s)	35, 157, 424, 1383
TaiI	ACGT	1 cut(s)	1176
TaqI	TCGA	4 cut(s)	151, 778, 841, 1299
TasI	AATT	5 cut(s)	1290, 1373, 1542, 1686, 1693
TatI	WGTACW	2 cut(s)	1452, 1679
TauI	GCSGC	1 cut(s)	300
TfiI	GAWTC	5 cut(s)	686, 896, 1067, 1074, 1233
Tru1I	TTAA	6 cut(s)	207, 582, 711, 1386, 1545, 1557
Tru9I	TTAA	6 cut(s)	207, 582, 711, 1386, 1545, 1557
TscAI	CASTG	3 cut(s)	370, 867, 1386
TseFI	GTSAC	3 cut(s)	343, 1249, 1526
TseI	GCWGC	3 cut(s)	371, 587, 724
Tsp45I	GTSAC	3 cut(s)	343, 1249, 1526
TspDTI	ATGAA	9 cut(s)	84, 260, 476, 1109, 1153, 1362, 1460, 1604, 1679
TspGWI	ACGGA	3 cut(s)	206, 1138, 1407
TspRI	CASTG	3 cut(s)	370, 867, 1386
Tth111I	GACNNNGTC	1 cut(s)	1532
Vha464I	CTTAAG	1 cut(s)	710
VpaK11BI	GGWCC	5 cut(s)	79, 660, 1093, 1324, 1534
XceI	RCATGY	1 cut(s)	1086
XcmI	CCANNNNNNNNNTGG	1 cut(s)	382
XmaJI	CCTAGG	1 cut(s)	1337
XmiI	GTMKAC	1 cut(s)	1645
XspI	CTAG	8 cut(s)	171, 186, 437, 756, 1184, 1338, 1515, 1583

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.