Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002464

Physical Location & Seq

Genomic Coordinates Forward (+)

15743812 .. 15746992

Gene Structure 3181 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00295700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGAAGGCGGCAACTCGTTACGCCCACAGCATTCCCAAGCTGGCCTGTCCGCGCGGCGAAGAAGGATTGATCATCAGCACCAAGAACCTCAACCGCGTGGTGGAGATCAACGTCGAAGAGCTGACGATGACGGTGGAGAGTGGCGCGACGCTTCGGCAGGTGATCAGTGAGGCGGCCAAGGTTGGCTTGGCCTTGCCGTACGCACCCTATTGGTGGGGGCTAACTATTGGTGGGTTGCTAGGAACTGGTGCGCATGGAAGCACATTATGGGGTAAGGGAAGCTCGGTTCATGATTATGTGGTGGGACTTGGGCTTGTGAGTCCAGGAGGGCCTGAAGATGGTCATGTCAAGGTGAGAAGTTTCGGTGAAGGTGATAAAGATATTAATGCAGCTAAGGTTTCACTTGGAGTCTTGGGGGTAATATCACAGACAACACTTTGGCAGGTTACTCTAAACCTACAACCCATGTTCAAGAGGTCCATCACGTATTTGAGCAAGAACGATTCTGACTTGGGAGATCAAGTGCTTAAGTTTGGCAAGCAGCATGAGTTTGCGGACATACTATGGTATCCTAGCCAGCACAAGGCAGTTTATCGAGTAGATGATCGTATCTCCTCAAACACATCCGGAAATGGTTTATATGATTTCCTTCCTTTTCGAGCAACACCTTCACTTGCACTGGCAGTTATAAGAACGGCAGAGGAGAACCAAGAATCCGAAAGTGATGCTGATGGGAAATGCTCTGGTGCAAAGTTGGTCACATCTGGGCTCCTTTCCAGTGCTTATGGTTTGACAAACAGTGGTATGATCTTTACTGGATATCCTGTGATTGGATATCAGAACCGGCTTCAAGCATCTGGAACTTGCCTTGATAGTCTTCAAGATTCAAAAATCACGGCATGCCCATGGGACTCAAGGATCAAGGGCGAGTTTTTTCATCAAACAACCTTCAGCATCAGCTTGTCCGTGGCCAAAAGCTTCATTCAAGACGTGCAGAAGCTAGTTGAGATTGAACCCAAATCCCTCTGTGGAGTTGAAATCTACAATGGAATCCTAATGCGCTATGTCACCGCATCAACTGCTTACCTTGGCAAACAAGAAGATGCAATTGACTTCGACATCACTTATTACCGGAGCAAGGACCCCATGACGCCTAGGCTCCATGAAGGCATACTTGAAGAAATAGAGCAATTGGCACTGTTTAAGTACGGGGCACTGCCGCATTGGGGAAAGAATAGGAACATAGCCTTTGATGGAGTGATGAAGAAGTACAAAAATGGTAAGGAGTTCTTGAGGGTTAAGGAAGTGTATGACCCATTGGGGCTATTCTCTAGTGAATGGAGTGACCAGGTCCTTGGAATTGAGGGTGAGGTGAACTTAGTCATGGAGGGCTGTGCGCTAGAAGGGCTGTGCATTTGCTCAGAGGACATTCATTGTGCCCCAAGCAAGGGGTATTTCTGTAGACCCGGCAAAGTGTTTAGGGATGCAAGGGTTTGCACACCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005618 GO:0005623 GO:0005975 GO:0005996 GO:0006082 GO:0006732 GO:0006766 GO:0006767 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016051 GO:0016053 GO:0016491 GO:0016614 GO:0016899 GO:0019752 GO:0019852 GO:0019853 GO:0030312 GO:0036094 GO:0042364 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044464 GO:0046364 GO:0046394 GO:0048037 GO:0050105 GO:0050660 GO:0050662 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:0071944 GO:0097159 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ALO FAD_binding_4

Protein Analysis

501

Amino Acids

54.95

Weight (kDa)

5.99

Isoelectric Point (pI)

36.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	16 - 110	2.7e-13	FAD binding domain
ALO	PF04030	312 - 450	3.1e-10	D-arabinono-1,4-lactone oxidase
GULLO2-like_3rd	PF22906	463 - 500	2.3e-24	L-gulonolactone oxidase 2-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46750 AT2G46760 AT5G56470
fragaria_vesca	FvH4_7g17510 FvH4_7g17511
malus_domestica	MD01G1087800.v1.1 MD07G1157900.v1.1
prunus_persica	Prupe.2G196400_v2.0.a1
pyrus_communis	pycom01g11170 pycom07g15450
rosa_chinensis	RchiOBHm_Chr1g0360921 RchiOBHm_Chr1g0360951 RchiOBHm_Chr3g0481291 RchiOBHm_Chr5g0029651
rosa_laevigata	RLG00000019622 RLG00000027767 RLG00000027769
rosa_multiflora	Rmu_sc0000206.1_g000019 Rmu_sc0000677.1_g000024 Rmu_sc0002207.1_g000003 Rmu_sc0008012.1_g000002
rosa_roxburghii	Rroxscaffold_3G00228090 Rroxscaffold_4G00295680 Rroxscaffold_4G00295700 Rroxscaffold_5G00385060
rosa_rugosa	Rorug01G0283500 Rorug01G0283800 Rorug03G0191000
rosa_samantha	Rh1AG295000 Rh1BG259800 Rh1BG259900 Rh1CG277200 Rh1DG289900 Rh2BG393900 Rh3AG241900 Rh3BG277100 Rh3DG268900
rosa_wichuraiana	Rw1G026070 Rw1G026100 Rw1G026110 Rw3G021610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	689
AarI	CACCTGC	1 cut(s)	148
Acc16I	TGCGCA	1 cut(s)	252
Acc36I	ACCTGC	2 cut(s)	148, 433
AccI	GTMKAC	1 cut(s)	1462
AccII	CGCG	4 cut(s)	52, 54, 96, 146
AccIII	TCCGGA	1 cut(s)	626
AciI	CCGC	8 cut(s)	8, 50, 54, 94, 173, 554, 1071, 1220
AclWI	GGATC	1 cut(s)	926
AcoI	YGGCCR	2 cut(s)	174, 969
AcuI	CTGAAG	2 cut(s)	354, 934
AcyI	GRCGYC	1 cut(s)	1151
AfaI	GTAC	3 cut(s)	200, 1208, 1271
AfiI	CCNNNNNNNGG	9 cut(s)	100, 213, 338, 583, 830, 1138, 1226, 1447, 1448
AflII	CTTAAG	1 cut(s)	527
AgsI	TTSAA	8 cut(s)	472, 851, 881, 888, 986, 1013, 1037, 1178
AjiI	CACGTC	1 cut(s)	991
AjnI	CCWGG	2 cut(s)	322, 1347
AloI	GAACNNNNNNTCC	4 cut(s)	270, 302, 698, 730
AluBI	AGCT	7 cut(s)	40, 121, 282, 392, 960, 978, 1000
AluI	AGCT	7 cut(s)	40, 121, 282, 392, 960, 978, 1000
AlwI	GGATC	1 cut(s)	926
Aor13HI	TCCGGA	1 cut(s)	626
AoxI	GGCC	5 cut(s)	42, 174, 189, 329, 969
ApeKI	GCWGC	2 cut(s)	389, 541
AseI	ATTAAT	1 cut(s)	384
AspA2I	CCTAGG	1 cut(s)	1154
AspLEI	GCGC	5 cut(s)	54, 146, 253, 1062, 1399
AspS9I	GGNCC	4 cut(s)	329, 477, 1141, 1351
AsuC2I	CCSGG	1 cut(s)	1467
AsuHPI	GGTGA	7 cut(s)	172, 364, 377, 383, 1060, 1379, 1384
AvaII	GGWCC	3 cut(s)	477, 1141, 1351
AvrII	CCTAGG	1 cut(s)	1154
BaeGI	GKGCMC	2 cut(s)	1216, 1441
BaeI	ACNNNNGTAYC	2 cut(s)	551, 584
BalI	TGGCCA	1 cut(s)	971
BanII	GRGCYC	1 cut(s)	771
BbsI	GAAGAC	1 cut(s)	869
BbvI	GCAGC	2 cut(s)	401, 553
BccI	CCATC	4 cut(s)	332, 488, 725, 1247
BceAI	ACGGC	3 cut(s)	181, 711, 912
BcgI	CGANNNNNNTGC	2 cut(s)	707, 741
BciT130I	CCWGG	2 cut(s)	324, 1349
BciVI	GTATCC	1 cut(s)	579
BclI	TGATCA	2 cut(s)	69, 162
BcnI	CCSGG	1 cut(s)	1467
BfaI	CTAG	6 cut(s)	239, 573, 1001, 1155, 1332, 1400
BfmI	CTRYAG	1 cut(s)	1459
BfrI	CTTAAG	1 cut(s)	527
BfuAI	ACCTGC	2 cut(s)	148, 433
BfuI	GTATCC	1 cut(s)	579
BisI	GCNGC	6 cut(s)	9, 55, 174, 390, 542, 1220
BlnI	CCTAGG	1 cut(s)	1154
BlsI	GCNGC	6 cut(s)	10, 56, 175, 391, 543, 1221
Bme1390I	CCNGG	3 cut(s)	324, 1349, 1467
Bme18I	GGWCC	3 cut(s)	477, 1141, 1351
BmgBI	CACGTC	1 cut(s)	991
BmgT120I	GGNCC	4 cut(s)	329, 477, 1141, 1351
BmiI	GGNNCC	3 cut(s)	770, 1143, 1160
BmrFI	CCNGG	3 cut(s)	324, 1349, 1467
BmsI	GCATC	6 cut(s)	715, 863, 963, 1082, 1093, 1474
BpiI	GAAGAC	1 cut(s)	869
Bpu10I	CCTNAGC	1 cut(s)	393
BpuEI	CTTGAG	2 cut(s)	898, 1312
BpuMI	CCSGG	1 cut(s)	1467
BsaAI	YACGTR	1 cut(s)	486
BsaHI	GRCGYC	1 cut(s)	1151
BsaJI	CCNNGG	6 cut(s)	177, 905, 966, 1087, 1154, 1354
BsaWI	WCCGGW	2 cut(s)	626, 1131
BsaXI	ACNNNNNCTCC	2 cut(s)	507, 537
Bsc4I	CCNNNNNNNGG	9 cut(s)	100, 213, 338, 583, 830, 1138, 1226, 1447, 1448
Bse118I	RCCGGY	1 cut(s)	843
Bse1I	ACTGG	4 cut(s)	250, 684, 777, 820
BseAI	TCCGGA	1 cut(s)	626
BseBI	CCWGG	2 cut(s)	324, 1349
BseDI	CCNNGG	6 cut(s)	177, 905, 966, 1087, 1154, 1354
BseGI	GGATG	2 cut(s)	623, 1489
BseLI	CCNNNNNNNGG	9 cut(s)	100, 213, 338, 583, 830, 1138, 1226, 1447, 1448
BseMII	CTCAG	1 cut(s)	1434
BseNI	ACTGG	4 cut(s)	250, 684, 777, 820
BseRI	GAGGAG	2 cut(s)	604, 716
BseSI	GKGCMC	2 cut(s)	1216, 1441
BseXI	GCAGC	2 cut(s)	401, 553
BsgI	GTGCAG	1 cut(s)	1013
Bsh1236I	CGCG	4 cut(s)	52, 54, 96, 146
BshFI	GGCC	5 cut(s)	44, 176, 191, 331, 971
BsiSI	CCGG	4 cut(s)	627, 844, 1132, 1467
BsiWI	CGTACG	1 cut(s)	198
BslFI	GGGAC	2 cut(s)	318, 923
BslI	CCNNNNNNNGG	9 cut(s)	100, 213, 338, 583, 830, 1138, 1226, 1447, 1448
BsmFI	GGGAC	2 cut(s)	318, 923
BsmI	GAATGC	1 cut(s)	30
BsnI	GGCC	5 cut(s)	44, 176, 191, 331, 971
Bsp1286I	GDGCHC	3 cut(s)	771, 1216, 1441
Bsp13I	TCCGGA	1 cut(s)	626
Bsp143I	GATC	7 cut(s)	69, 105, 162, 517, 604, 807, 918
Bsp19I	CCATGG	1 cut(s)	905
BspACI	CCGC	8 cut(s)	8, 50, 54, 94, 173, 554, 1071, 1220
BspANI	GGCC	5 cut(s)	44, 176, 191, 331, 971
BspCNI	CTCAG	1 cut(s)	1433
BspEI	TCCGGA	1 cut(s)	626
BspFNI	CGCG	4 cut(s)	52, 54, 96, 146
BspHI	TCATGA	1 cut(s)	289
BspLI	GGNNCC	3 cut(s)	770, 1143, 1160
BspMI	ACCTGC	2 cut(s)	148, 433
BspPI	GGATC	1 cut(s)	926
BspQI	GCTCTTC	1 cut(s)	111
BspTI	CTTAAG	1 cut(s)	527
BsrFI	RCCGGY	1 cut(s)	843
BsrI	ACTGG	4 cut(s)	250, 684, 777, 820
BssAI	RCCGGY	1 cut(s)	843
BssECI	CCNNGG	6 cut(s)	177, 905, 966, 1087, 1154, 1354
BssMI	GATC	7 cut(s)	69, 105, 162, 517, 604, 807, 918
BssNI	GRCGYC	1 cut(s)	1151
BssT1I	CCWWGG	5 cut(s)	177, 905, 1087, 1154, 1354
Bst2UI	CCWGG	2 cut(s)	324, 1349
Bst4CI	ACNGT	3 cut(s)	133, 800, 1200
Bst6I	CTCTTC	1 cut(s)	111
BstACI	GRCGYC	1 cut(s)	1151
BstAFI	CTTAAG	1 cut(s)	527
BstAPI	GCANNNNNTGC	1 cut(s)	1079
BstBAI	YACGTR	1 cut(s)	486
BstC8I	GCNNGC	4 cut(s)	42, 539, 578, 901
BstDEI	CTNAG	3 cut(s)	393, 1378, 1420
BstDSI	CCRYGG	2 cut(s)	905, 966
BstF5I	GGATG	2 cut(s)	623, 1489
BstFNI	CGCG	4 cut(s)	52, 54, 96, 146
BstHHI	GCGC	5 cut(s)	54, 146, 253, 1062, 1399
BstKTI	GATC	7 cut(s)	72, 108, 165, 520, 607, 810, 921
BstMBI	GATC	7 cut(s)	69, 105, 162, 517, 604, 807, 918
BstMWI	GCNNNNNNNGC	2 cut(s)	1079, 1405
BstNI	CCWGG	2 cut(s)	324, 1349
BstNSI	RCATGY	1 cut(s)	903
BstSCI	CCNGG	3 cut(s)	322, 1347, 1465
BstSFI	CTRYAG	1 cut(s)	1459
BstSLI	GKGCMC	2 cut(s)	1216, 1441
BstUI	CGCG	4 cut(s)	52, 54, 96, 146
BstV1I	GCAGC	2 cut(s)	401, 553
BstV2I	GAAGAC	1 cut(s)	869
BstXI	CCANNNNNNTGG	1 cut(s)	1355
BsuI	GTATCC	1 cut(s)	579
BsuRI	GGCC	5 cut(s)	44, 176, 191, 331, 971
BtgI	CCRYGG	2 cut(s)	905, 966
BtrI	CACGTC	1 cut(s)	991
BtsCI	GGATG	2 cut(s)	623, 1489
BtsI	GCAGTG	1 cut(s)	1214
BtsIMutI	CAGTG	6 cut(s)	172, 677, 784, 805, 1196, 1214
BveI	ACCTGC	2 cut(s)	148, 433
Cac8I	GCNNGC	4 cut(s)	42, 539, 578, 901
CciI	TCATGA	1 cut(s)	289
CfoI	GCGC	5 cut(s)	54, 146, 253, 1062, 1399
Cfr10I	RCCGGY	1 cut(s)	843
Cfr13I	GGNCC	4 cut(s)	329, 477, 1141, 1351
CseI	GACGC	2 cut(s)	157, 1159
CsiI	ACCWGGT	1 cut(s)	1347
Csp6I	GTAC	3 cut(s)	199, 1207, 1270
CviQI	GTAC	3 cut(s)	199, 1207, 1270
DdeI	CTNAG	3 cut(s)	393, 1378, 1420
DpnI	GATC	7 cut(s)	71, 107, 164, 519, 606, 809, 920
DpnII	GATC	7 cut(s)	69, 105, 162, 517, 604, 807, 918
EaeI	YGGCCR	2 cut(s)	174, 969
Eam1104I	CTCTTC	1 cut(s)	111
EarI	CTCTTC	1 cut(s)	111
Eco130I	CCWWGG	5 cut(s)	177, 905, 1087, 1154, 1354
Eco24I	GRGCYC	1 cut(s)	771
Eco32I	GATATC	2 cut(s)	821, 836
Eco47I	GGWCC	3 cut(s)	477, 1141, 1351
Eco57I	CTGAAG	2 cut(s)	354, 934
EcoO109I	RGGNCCY	3 cut(s)	329, 1141, 1351
EcoRII	CCWGG	2 cut(s)	322, 1347
EcoRV	GATATC	2 cut(s)	821, 836
EcoT14I	CCWWGG	5 cut(s)	177, 905, 1087, 1154, 1354
EcoT38I	GRGCYC	1 cut(s)	771
ErhI	CCWWGG	5 cut(s)	177, 905, 1087, 1154, 1354
FalI	AAGNNNNNCTT	6 cut(s)	170, 202, 1158, 1190, 1274, 1306
FaqI	GGGAC	2 cut(s)	318, 923
FbaI	TGATCA	2 cut(s)	69, 162
FblI	GTMKAC	1 cut(s)	1462
Fnu4HI	GCNGC	6 cut(s)	9, 55, 174, 390, 542, 1220
FokI	GGATG	2 cut(s)	610, 1496
FriOI	GRGCYC	1 cut(s)	771
Fsp4HI	GCNGC	6 cut(s)	9, 55, 174, 390, 542, 1220
FspAI	RTGCGCAY	1 cut(s)	252
FspBI	CTAG	6 cut(s)	239, 573, 1001, 1155, 1332, 1400
FspI	TGCGCA	1 cut(s)	252
GlaI	GCGC	5 cut(s)	53, 145, 252, 1061, 1398
GluI	GCNGC	6 cut(s)	9, 55, 174, 390, 542, 1220
HaeIII	GGCC	5 cut(s)	44, 176, 191, 331, 971
HapII	CCGG	4 cut(s)	627, 844, 1132, 1467
HgaI	GACGC	2 cut(s)	157, 1159
HhaI	GCGC	5 cut(s)	54, 146, 253, 1062, 1399
Hin1I	GRCGYC	1 cut(s)	1151
Hin6I	GCGC	5 cut(s)	52, 144, 251, 1060, 1397
HinP1I	GCGC	5 cut(s)	52, 144, 251, 1060, 1397
HindIII	AAGCTT	1 cut(s)	976
HinfI	GANTC	7 cut(s)	319, 408, 503, 713, 884, 911, 1050
HpaII	CCGG	4 cut(s)	627, 844, 1132, 1467
HphI	GGTGA	7 cut(s)	172, 364, 377, 383, 1060, 1379, 1384
Hpy166II	GTNNAC	2 cut(s)	1375, 1463
Hpy188I	TCNGA	4 cut(s)	508, 718, 840, 1423
Hpy188III	TCNNGA	7 cut(s)	290, 472, 627, 858, 881, 986, 1291
Hpy8I	GTNNAC	2 cut(s)	1375, 1463
Hpy99I	CGWCG	2 cut(s)	116, 151
HpyAV	CCTTC	7 cut(s)	56, 362, 659, 678, 958, 1160, 1397
HpyCH4III	ACNGT	3 cut(s)	133, 800, 1200
HpyCH4IV	ACGT	3 cut(s)	111, 485, 990
HpyCH4V	TGCA	8 cut(s)	389, 677, 749, 994, 1106, 1413, 1487, 1497
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1079, 1405
HpyF3I	CTNAG	3 cut(s)	393, 1378, 1420
HpySE526I	ACGT	3 cut(s)	111, 485, 990
Hsp92I	GRCGYC	1 cut(s)	1151
HspAI	GCGC	5 cut(s)	52, 144, 251, 1060, 1397
Kpn2I	TCCGGA	1 cut(s)	626
Ksp22I	TGATCA	2 cut(s)	69, 162
Kzo9I	GATC	7 cut(s)	69, 105, 162, 517, 604, 807, 918
LguI	GCTCTTC	1 cut(s)	111
LmnI	GCTCC	3 cut(s)	774, 1134, 1164
Lsp1109I	GCAGC	2 cut(s)	401, 553
LweI	GCATC	6 cut(s)	715, 863, 963, 1082, 1093, 1474
MabI	ACCWGGT	1 cut(s)	1347
MaeI	CTAG	6 cut(s)	239, 573, 1001, 1155, 1332, 1400
MaeII	ACGT	3 cut(s)	111, 485, 990
MaeIII	GTNAC	5 cut(s)	17, 445, 757, 1066, 1343
MalI	GATC	7 cut(s)	71, 107, 164, 519, 606, 809, 920
MboI	GATC	7 cut(s)	69, 105, 162, 517, 604, 807, 918
MboII	GAAGA	7 cut(s)	71, 128, 347, 869, 1112, 1190, 1276
MfeI	CAATTG	2 cut(s)	1107, 1190
MhlI	GDGCHC	3 cut(s)	771, 1216, 1441
MlsI	TGGCCA	1 cut(s)	971
MluCI	AATT	3 cut(s)	1107, 1190, 1359
MluNI	TGGCCA	1 cut(s)	971
MlyI	GAGTC	3 cut(s)	328, 417, 905
Mox20I	TGGCCA	1 cut(s)	971
MroI	TCCGGA	1 cut(s)	626
MscI	TGGCCA	1 cut(s)	971
MseI	TTAA	4 cut(s)	384, 528, 1203, 1299
MslI	CAYNNNNRTG	2 cut(s)	294, 904
Msp20I	TGGCCA	1 cut(s)	971
MspCI	CTTAAG	1 cut(s)	527
MspI	CCGG	4 cut(s)	627, 844, 1132, 1467
MspR9I	CCNGG	3 cut(s)	324, 1349, 1467
MunI	CAATTG	2 cut(s)	1107, 1190
Mva1269I	GAATGC	1 cut(s)	30
MvaI	CCWGG	2 cut(s)	324, 1349
MvnI	CGCG	4 cut(s)	52, 54, 96, 146
MwoI	GCNNNNNNNGC	2 cut(s)	1079, 1405
NciI	CCSGG	1 cut(s)	1467
NcoI	CCATGG	1 cut(s)	905
NdeII	GATC	7 cut(s)	69, 105, 162, 517, 604, 807, 918
NlaIV	GGNNCC	3 cut(s)	770, 1143, 1160
NmuCI	GTSAC	3 cut(s)	757, 1066, 1343
NsbI	TGCGCA	1 cut(s)	252
NspI	RCATGY	1 cut(s)	903
PaeI	GCATGC	1 cut(s)	903
PagI	TCATGA	1 cut(s)	289
PaqCI	CACCTGC	1 cut(s)	148
PciSI	GCTCTTC	1 cut(s)	111
PctI	GAATGC	1 cut(s)	30
PfeI	GAWTC	4 cut(s)	503, 713, 884, 1050
Pfl23II	CGTACG	1 cut(s)	198
PflFI	GACNNNGTC	1 cut(s)	1349
PfoI	TCCNGGA	1 cut(s)	322
PkrI	GCNGC	6 cut(s)	10, 56, 175, 391, 543, 1221
PleI	GAGTC	3 cut(s)	327, 416, 905
PpsI	GAGTC	3 cut(s)	327, 416, 905
Ppu21I	YACGTR	1 cut(s)	486
PpuMI	RGGWCCY	2 cut(s)	1141, 1351
PshBI	ATTAAT	1 cut(s)	384
PsiI	TTATAA	1 cut(s)	689
Psp5II	RGGWCCY	2 cut(s)	1141, 1351
Psp6I	CCWGG	2 cut(s)	322, 1347
PspGI	CCWGG	2 cut(s)	322, 1347
PspLI	CGTACG	1 cut(s)	198
PspN4I	GGNNCC	3 cut(s)	770, 1143, 1160
PspPI	GGNCC	4 cut(s)	329, 477, 1141, 1351
PspPPI	RGGWCCY	2 cut(s)	1141, 1351
PsyI	GACNNNGTC	1 cut(s)	1349
RsaI	GTAC	3 cut(s)	200, 1208, 1271
RsaNI	GTAC	3 cut(s)	199, 1207, 1270
RseI	CAYNNNNRTG	2 cut(s)	294, 904
SapI	GCTCTTC	1 cut(s)	111
SaqAI	TTAA	4 cut(s)	384, 528, 1203, 1299
SatI	GCNGC	6 cut(s)	9, 55, 174, 390, 542, 1220
Sau3AI	GATC	7 cut(s)	69, 105, 162, 517, 604, 807, 918
Sau96I	GGNCC	4 cut(s)	329, 477, 1141, 1351
SchI	GAGTC	3 cut(s)	328, 417, 905
ScrFI	CCNGG	3 cut(s)	324, 1349, 1467
SduI	GDGCHC	3 cut(s)	771, 1216, 1441
SexAI	ACCWGGT	1 cut(s)	1347
SfaNI	GCATC	6 cut(s)	715, 863, 963, 1082, 1093, 1474
SfcI	CTRYAG	1 cut(s)	1459
SinI	GGWCC	3 cut(s)	477, 1141, 1351
SmiMI	CAYNNNNRTG	2 cut(s)	294, 904
SmlI	CTYRAG	3 cut(s)	527, 913, 1291
SmoI	CTYRAG	3 cut(s)	527, 913, 1291
SphI	GCATGC	1 cut(s)	903
Sse9I	AATT	3 cut(s)	1107, 1190, 1359
SsiI	CCGC	8 cut(s)	8, 50, 54, 94, 173, 554, 1071, 1220
SspMI	CTAG	6 cut(s)	239, 573, 1001, 1155, 1332, 1400
StyD4I	CCNGG	3 cut(s)	322, 1347, 1465
StyI	CCWWGG	5 cut(s)	177, 905, 1087, 1154, 1354
TaaI	ACNGT	3 cut(s)	133, 800, 1200
TaiI	ACGT	3 cut(s)	114, 488, 993
TaqI	TCGA	4 cut(s)	114, 595, 658, 1116
TasI	AATT	3 cut(s)	1107, 1190, 1359
TatI	WGTACW	1 cut(s)	1269
TauI	GCSGC	4 cut(s)	11, 57, 176, 1222
TfiI	GAWTC	4 cut(s)	503, 713, 884, 1050
Tru1I	TTAA	4 cut(s)	384, 528, 1203, 1299
Tru9I	TTAA	4 cut(s)	384, 528, 1203, 1299
TscAI	CASTG	6 cut(s)	172, 684, 784, 805, 1203, 1221
TseFI	GTSAC	3 cut(s)	757, 1066, 1343
TseI	GCWGC	2 cut(s)	389, 541
Tsp45I	GTSAC	3 cut(s)	757, 1066, 1343
TspDTI	ATGAA	7 cut(s)	17, 278, 926, 970, 1179, 1277, 1421
TspGWI	ACGGA	1 cut(s)	955
TspRI	CASTG	6 cut(s)	172, 684, 784, 805, 1203, 1221
Tth111I	GACNNNGTC	1 cut(s)	1349
Vha464I	CTTAAG	1 cut(s)	527
VpaK11BI	GGWCC	3 cut(s)	477, 1141, 1351
VspI	ATTAAT	1 cut(s)	384
XceI	RCATGY	1 cut(s)	903
XcmI	CCANNNNNNNNNTGG	1 cut(s)	184
XmaJI	CCTAGG	1 cut(s)	1154
XmiI	GTMKAC	1 cut(s)	1462
XspI	CTAG	6 cut(s)	239, 573, 1001, 1155, 1332, 1400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.