Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0002464

Physical Location & Seq

Genomic Coordinates Forward (+)

15721899 .. 15727827

Gene Structure 5929 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00295680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGACTGTGACTTTGATTGAAGTTGCATTAATCCCTTACATTACTGCCGGTGATCCTGATCTCTCAGTCACCGCAGAAGCATTGAAGGTCCTGGATTCTTGTGGATCAGACATAATTGAATTAGGCGTGCCATACTCTGATTCATTAGCGGATGGGCCAGTTATACAGGACCACATCAAGTGTTCATCGTCAAACAACACAAACTGCACCATCACAAACTCCTACGGCATCTTCCCCGATCGAACCGTCTGTAAAGCGACTAGTGTATCATACCCTAGTACGGAGGAAGAACTCGTTAAGGTAGTGGCTGATGCAACCAAGAACAAGAAGAGCATGAAGGTGGCGACTCGTTACTCCCACAGCATTCCCAAGCTGGTTTGCCCGCGCGGCGAAGATGGGCTGATCATAAGCACCAACAACCTCAACCGTGTGGTTAAGGTCGACGTTGAAGGGATGACGATGACAGTGGAGAGCGGCGTGACTCTTAGGCAGGTGATCAGTGAGGCTGCCAAGGTTGGCTTGGCGTTGCCTTACGCACCATATTGGTGGGGGCTAACTGTCGGTGGGTTGCTAGGGACTGGTGCGCATGGAAGCACATTATGGGCTAAGGGAAGCTCTGTTCATGATTATGTGGTGGGACTTAGGATTGTGAGTCCAGGAGGGCCCGAAGATGGTTATGTGAAGGTGAGAAGTCTCAGTCAAGGTGATAAAGATCTTAATGCAGCTAAGGTTTCACTTGGAGTCTTAGGAACAAACAAGTCTAAGAGACAAAAACTTTGGCAGGTTACTCTAAACCTACAACCCATGTTCAAGAGGTCCATCACATATTTGAGCAAGAACGATTCTGACTTGGGAGATCAAGTGCTTAAGTTTGGCAAGCAGCATGAGTTTGCGGACATACTATGGTATCCTAGCCAGCACAAGGCAGTTTATCGAGTAGATGATCGTATCTCCTCAAACACATCCGGAAATGGTTTATATGATTTCCTTCCTTTTCGAGCAACACCTTCACTTGCACTGGCAGTTATAAGAACGGCAGAAGAGAACCAAGAATCAAAAAGCGATGCTGATGGGAAATGCTCTGGGGCAAAGTTGGTCACATCTGGGCTTCTATCCGATGCTTATGGTTTGACAAACAATGGTATAATCTTTACTGGATATCCTGTGATTGGATATCAGAACCGGCTTCAAGCATCTGGAACTTGCCTTGATAGTCTTCAAGATTCAAGAATCACAGCATGCGCATGGGACCCAAGGATCAAGGGCGAGTTTTTTCATCAAACAACCTTCAGCATCAGCTTGTCCTTGGCCAAAAGCTTCATTCAAGACGTGCAGAAGCTAGTTGAGATTGAACCCAAATCCCTCTGTGGAGTAGAAATCTACAATGGAATCCTAATGCGCTATGTCACCGCATCAACTGCTTACCTTGGCAAACAAGTAGATGCAATTGACTTCGACATCACTTATTACCGAAGCAAGGACCCCATGAAGCCTAGGCTCCATGAAGGCATACTTGAAGAAATAGAGCAATTGGCACTGTTTAAGTACGGAGCATTGCCACATTGGGGAAAGAATAGGAACATAGCCTTTGATGGGGTGATGAAGAAGTACAAAAATGGTAAGGAGTTCTTGAGGGTTAAGGAAGTGTATGACCCAATGGGGCTATTCTCAAGTGAATGGAGTGACCAGGTCCTTGGAATTAAGGGTGAGGTTAACTTAGTCAAGGAAGGGTGCGCCCTAGAAGGGTTGTGCATTTGCTCACTAGACATTCATTGTGCCCCAAGCAAGGGCTACTTCTGTAGACCGGGGAAAGTATTTAGGGATGCAAGGATCTGTACTCCGAATCATAATTCGACAAGAACCACAAGTAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005618 GO:0005623 GO:0005975 GO:0005996 GO:0006082 GO:0006732 GO:0006766 GO:0006767 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016051 GO:0016053 GO:0016491 GO:0016614 GO:0016899 GO:0019752 GO:0019852 GO:0019853 GO:0030312 GO:0036094 GO:0042364 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044464 GO:0046364 GO:0046394 GO:0048037 GO:0050105 GO:0050660 GO:0050662 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:0071944 GO:0097159 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ALO FAD_binding_4

Protein Analysis

624

Amino Acids

68.34

Weight (kDa)

7.8

Isoelectric Point (pI)

29.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trp_syntA	PF00290	8 - 62	1.6e-18	Tryptophan synthase alpha chain
FAD_binding_4	PF01565	89 - 221	6.6e-20	FAD binding domain
ALO	PF04030	425 - 563	1.4e-09	D-arabinono-1,4-lactone oxidase
GULLO2-like_3rd	PF22906	575 - 613	4.5e-23	L-gulonolactone oxidase 2-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002464)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46750 AT2G46760 AT5G56470
fragaria_vesca	FvH4_7g17510 FvH4_7g17511
malus_domestica	MD01G1087800.v1.1 MD07G1157900.v1.1
prunus_persica	Prupe.2G196400_v2.0.a1
pyrus_communis	pycom01g11170 pycom07g15450
rosa_chinensis	RchiOBHm_Chr1g0360921 RchiOBHm_Chr1g0360951 RchiOBHm_Chr3g0481291 RchiOBHm_Chr5g0029651
rosa_laevigata	RLG00000019622 RLG00000027767 RLG00000027769
rosa_multiflora	Rmu_sc0000206.1_g000019 Rmu_sc0000677.1_g000024 Rmu_sc0002207.1_g000003 Rmu_sc0008012.1_g000002
rosa_roxburghii	Rroxscaffold_3G00228090 Rroxscaffold_4G00295680 Rroxscaffold_4G00295700 Rroxscaffold_5G00385060
rosa_rugosa	Rorug01G0283500 Rorug01G0283800 Rorug03G0191000
rosa_samantha	Rh1AG295000 Rh1BG259800 Rh1BG259900 Rh1CG277200 Rh1DG289900 Rh2BG393900 Rh3AG241900 Rh3BG277100 Rh3DG268900
rosa_wichuraiana	Rw1G026070 Rw1G026100 Rw1G026110 Rw3G021610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1028
AarI	CACCTGC	1 cut(s)	481
Acc16I	TGCGCA	2 cut(s)	585, 1243
Acc36I	ACCTGC	2 cut(s)	481, 772
AccBSI	CCGCTC	1 cut(s)	474
AccI	GTMKAC	2 cut(s)	441, 1801
AccII	CGCG	2 cut(s)	385, 387
AccIII	TCCGGA	1 cut(s)	965
AciI	CCGC	7 cut(s)	72, 149, 383, 387, 474, 893, 1410
AclWI	GGATC	4 cut(s)	47, 112, 1265, 1838
AcoI	YGGCCR	1 cut(s)	1308
AcuI	CTGAAG	1 cut(s)	1273
AfaI	GTAC	4 cut(s)	280, 1547, 1610, 1837
AflII	CTTAAG	1 cut(s)	866
AhlI	ACTAGT	1 cut(s)	260
AjiI	CACGTC	1 cut(s)	1330
AjnI	CCWGG	3 cut(s)	90, 655, 1686
AloI	GAACNNNNNNTCC	2 cut(s)	603, 635
AluBI	AGCT	6 cut(s)	373, 615, 725, 1299, 1317, 1339
AluI	AGCT	6 cut(s)	373, 615, 725, 1299, 1317, 1339
Alw26I	GTCTC	2 cut(s)	698, 760
AlwI	GGATC	4 cut(s)	47, 112, 1265, 1838
Aor13HI	TCCGGA	1 cut(s)	965
AoxI	GGCC	3 cut(s)	155, 662, 1308
ApaI	GGGCCC	1 cut(s)	666
ApeKI	GCWGC	3 cut(s)	506, 722, 880
ArsI	GACNNNNNNTTYG	3 cut(s)	27, 759, 791
AseI	ATTAAT	1 cut(s)	29
AspA2I	CCTAGG	1 cut(s)	1493
AspLEI	GCGC	5 cut(s)	387, 586, 1244, 1401, 1736
AspS9I	GGNCC	9 cut(s)	88, 155, 169, 662, 663, 816, 1249, 1480, 1690
AsuC2I	CCSGG	1 cut(s)	1806
AsuHPI	GGTGA	8 cut(s)	61, 62, 505, 697, 716, 1399, 1609, 1718
AvaII	GGWCC	6 cut(s)	88, 169, 816, 1249, 1480, 1690
AvrII	CCTAGG	1 cut(s)	1493
BaeGI	GKGCMC	2 cut(s)	666, 1780
BaeI	ACNNNNGTAYC	4 cut(s)	249, 282, 890, 923
BalI	TGGCCA	1 cut(s)	1310
BanII	GRGCYC	1 cut(s)	666
BbsI	GAAGAC	1 cut(s)	1208
BbvI	GCAGC	3 cut(s)	493, 734, 892
BccI	CCATC	7 cut(s)	146, 218, 389, 665, 827, 1064, 1586
BceAI	ACGGC	2 cut(s)	241, 1050
BciT130I	CCWGG	3 cut(s)	92, 657, 1688
BciVI	GTATCC	1 cut(s)	918
BclI	TGATCA	2 cut(s)	402, 495
BcnI	CCSGG	1 cut(s)	1806
BcoDI	GTCTC	2 cut(s)	698, 760
BcuI	ACTAGT	1 cut(s)	260
BfaI	CTAG	9 cut(s)	261, 276, 572, 912, 1340, 1494, 1739, 1763, 1873
BfmI	CTRYAG	1 cut(s)	1798
BfrI	CTTAAG	1 cut(s)	866
BfuAI	ACCTGC	2 cut(s)	481, 772
BfuI	GTATCC	1 cut(s)	918
BglI	GCCNNNNNGGC	1 cut(s)	387
BglII	AGATCT	1 cut(s)	712
BisI	GCNGC	5 cut(s)	388, 475, 507, 723, 881
BlnI	CCTAGG	1 cut(s)	1493
BlsI	GCNGC	5 cut(s)	389, 476, 508, 724, 882
Bme1390I	CCNGG	4 cut(s)	92, 657, 1688, 1806
Bme18I	GGWCC	6 cut(s)	88, 169, 816, 1249, 1480, 1690
BmgBI	CACGTC	1 cut(s)	1330
BmgT120I	GGNCC	9 cut(s)	88, 155, 169, 662, 663, 816, 1249, 1480, 1690
BmiI	GGNNCC	5 cut(s)	664, 1250, 1251, 1482, 1499
BmrFI	CCNGG	4 cut(s)	92, 657, 1688, 1806
BmsI	GCATC	9 cut(s)	237, 301, 1054, 1108, 1202, 1302, 1421, 1432, 1813
BpiI	GAAGAC	1 cut(s)	1208
Bpu10I	CCTNAGC	2 cut(s)	606, 726
BpuEI	CTTGAG	2 cut(s)	1651, 1654
BpuMI	CCSGG	1 cut(s)	1806
BsaBI	GATNNNNATC	2 cut(s)	57, 711
BsaJI	CCNNGG	7 cut(s)	510, 1253, 1305, 1426, 1493, 1693, 1805
BsaWI	WCCGGW	1 cut(s)	965
BsaXI	ACNNNNNCTCC	2 cut(s)	846, 876
Bse118I	RCCGGY	2 cut(s)	47, 1182
Bse1I	ACTGG	4 cut(s)	158, 583, 1023, 1159
Bse3DI	GCAATG	1 cut(s)	1553
Bse8I	GATNNNNATC	2 cut(s)	57, 711
BseAI	TCCGGA	1 cut(s)	965
BseBI	CCWGG	3 cut(s)	92, 657, 1688
BseDI	CCNNGG	7 cut(s)	510, 1253, 1305, 1426, 1493, 1693, 1805
BseGI	GGATG	4 cut(s)	157, 459, 962, 1828
BseJI	GATNNNNATC	2 cut(s)	57, 711
BseMI	GCAATG	1 cut(s)	1553
BseMII	CTCAG	2 cut(s)	78, 709
BseNI	ACTGG	4 cut(s)	158, 583, 1023, 1159
BseRI	GAGGAG	1 cut(s)	943
BseSI	GKGCMC	2 cut(s)	666, 1780
BseXI	GCAGC	3 cut(s)	493, 734, 892
BsgI	GTGCAG	2 cut(s)	190, 1352
Bsh1236I	CGCG	2 cut(s)	385, 387
Bsh1285I	CGRYCG	1 cut(s)	241
BshFI	GGCC	3 cut(s)	157, 664, 1310
BsiEI	CGRYCG	1 cut(s)	241
BsiSI	CCGG	4 cut(s)	48, 966, 1183, 1805
BslFI	GGGAC	3 cut(s)	589, 651, 1262
BsmAI	GTCTC	2 cut(s)	698, 760
BsmFI	GGGAC	3 cut(s)	589, 651, 1262
BsmI	GAATGC	1 cut(s)	363
BsnI	GGCC	3 cut(s)	157, 664, 1310
Bsp120I	GGGCCC	1 cut(s)	662
Bsp1286I	GDGCHC	2 cut(s)	666, 1780
Bsp13I	TCCGGA	1 cut(s)	965
BspACI	CCGC	7 cut(s)	72, 149, 383, 387, 474, 893, 1410
BspANI	GGCC	3 cut(s)	157, 664, 1310
BspCNI	CTCAG	2 cut(s)	77, 708
BspEI	TCCGGA	1 cut(s)	965
BspFNI	CGCG	2 cut(s)	385, 387
BspHI	TCATGA	1 cut(s)	622
BspLI	GGNNCC	5 cut(s)	664, 1250, 1251, 1482, 1499
BspMI	ACCTGC	2 cut(s)	481, 772
BspPI	GGATC	4 cut(s)	47, 112, 1265, 1838
BspQI	GCTCTTC	1 cut(s)	323
BspTI	CTTAAG	1 cut(s)	866
BsrBI	CCGCTC	1 cut(s)	474
BsrDI	GCAATG	1 cut(s)	1553
BsrFI	RCCGGY	2 cut(s)	47, 1182
BsrI	ACTGG	4 cut(s)	158, 583, 1023, 1159
BssAI	RCCGGY	2 cut(s)	47, 1182
BssECI	CCNNGG	7 cut(s)	510, 1253, 1305, 1426, 1493, 1693, 1805
BssT1I	CCWWGG	6 cut(s)	510, 1253, 1305, 1426, 1493, 1693
Bst2UI	CCWGG	3 cut(s)	92, 657, 1688
Bst4CI	ACNGT	6 cut(s)	7, 247, 428, 466, 559, 1539
Bst6I	CTCTTC	2 cut(s)	323, 1035
BstAFI	CTTAAG	1 cut(s)	866
BstAPI	GCANNNNNTGC	1 cut(s)	1418
BstC8I	GCNNGC	5 cut(s)	128, 383, 878, 917, 1240
BstDEI	CTNAG	9 cut(s)	64, 485, 606, 641, 695, 726, 745, 762, 1717
BstF5I	GGATG	4 cut(s)	157, 459, 962, 1828
BstFNI	CGCG	2 cut(s)	385, 387
BstHHI	GCGC	5 cut(s)	387, 586, 1244, 1401, 1736
BstMAI	GTCTC	2 cut(s)	698, 760
BstMCI	CGRYCG	1 cut(s)	241
BstMWI	GCNNNNNNNGC	2 cut(s)	387, 1418
BstNI	CCWGG	3 cut(s)	92, 657, 1688
BstNSI	RCATGY	1 cut(s)	1242
BstSCI	CCNGG	4 cut(s)	90, 655, 1686, 1804
BstSFI	CTRYAG	1 cut(s)	1798
BstSLI	GKGCMC	2 cut(s)	666, 1780
BstUI	CGCG	2 cut(s)	385, 387
BstV1I	GCAGC	3 cut(s)	493, 734, 892
BstV2I	GAAGAC	1 cut(s)	1208
BstX2I	RGATCY	2 cut(s)	712, 1830
BstXI	CCANNNNNNTGG	2 cut(s)	546, 1694
BstYI	RGATCY	2 cut(s)	712, 1830
BsuI	GTATCC	1 cut(s)	918
BsuRI	GGCC	3 cut(s)	157, 664, 1310
BtgZI	GCGATG	1 cut(s)	1077
BtrI	CACGTC	1 cut(s)	1330
BtsCI	GGATG	4 cut(s)	157, 459, 962, 1828
BtsIMutI	CAGTG	4 cut(s)	471, 505, 1016, 1535
BveI	ACCTGC	2 cut(s)	481, 772
Cac8I	GCNNGC	5 cut(s)	128, 383, 878, 917, 1240
CciI	TCATGA	1 cut(s)	622
CfoI	GCGC	5 cut(s)	387, 586, 1244, 1401, 1736
Cfr10I	RCCGGY	2 cut(s)	47, 1182
Cfr13I	GGNCC	9 cut(s)	88, 155, 169, 662, 663, 816, 1249, 1480, 1690
CsiI	ACCWGGT	1 cut(s)	1686
Csp6I	GTAC	4 cut(s)	279, 1546, 1609, 1836
CviAII	CATG	9 cut(s)	334, 587, 623, 805, 884, 1239, 1245, 1486, 1502
CviQI	GTAC	4 cut(s)	279, 1546, 1609, 1836
DdeI	CTNAG	9 cut(s)	64, 485, 606, 641, 695, 726, 745, 762, 1717
EaeI	YGGCCR	1 cut(s)	1308
Eam1104I	CTCTTC	2 cut(s)	323, 1035
EarI	CTCTTC	2 cut(s)	323, 1035
Eco130I	CCWWGG	6 cut(s)	510, 1253, 1305, 1426, 1493, 1693
Eco24I	GRGCYC	1 cut(s)	666
Eco32I	GATATC	2 cut(s)	1160, 1175
Eco47I	GGWCC	6 cut(s)	88, 169, 816, 1249, 1480, 1690
Eco57I	CTGAAG	1 cut(s)	1273
EcoO109I	RGGNCCY	5 cut(s)	88, 662, 1249, 1480, 1690
EcoRII	CCWGG	3 cut(s)	90, 655, 1686
EcoRV	GATATC	2 cut(s)	1160, 1175
EcoT14I	CCWWGG	6 cut(s)	510, 1253, 1305, 1426, 1493, 1693
EcoT38I	GRGCYC	1 cut(s)	666
ErhI	CCWWGG	6 cut(s)	510, 1253, 1305, 1426, 1493, 1693
FaeI	CATG	9 cut(s)	337, 590, 626, 808, 887, 1242, 1248, 1489, 1505
FalI	AAGNNNNNCTT	8 cut(s)	503, 535, 1497, 1529, 1613, 1645, 1778, 1810
FaqI	GGGAC	3 cut(s)	589, 651, 1262
FatI	CATG	9 cut(s)	333, 586, 622, 804, 883, 1238, 1244, 1485, 1501
FauI	CCCGC	1 cut(s)	390
FbaI	TGATCA	2 cut(s)	402, 495
FblI	GTMKAC	2 cut(s)	441, 1801
Fnu4HI	GCNGC	5 cut(s)	388, 475, 507, 723, 881
FokI	GGATG	4 cut(s)	164, 466, 949, 1835
FriOI	GRGCYC	1 cut(s)	666
Fsp4HI	GCNGC	5 cut(s)	388, 475, 507, 723, 881
FspAI	RTGCGCAY	2 cut(s)	585, 1243
FspBI	CTAG	9 cut(s)	261, 276, 572, 912, 1340, 1494, 1739, 1763, 1873
FspI	TGCGCA	2 cut(s)	585, 1243
GlaI	GCGC	5 cut(s)	386, 585, 1243, 1400, 1735
GluI	GCNGC	5 cut(s)	388, 475, 507, 723, 881
HaeIII	GGCC	3 cut(s)	157, 664, 1310
HapII	CCGG	4 cut(s)	48, 966, 1183, 1805
HhaI	GCGC	5 cut(s)	387, 586, 1244, 1401, 1736
Hin1II	CATG	9 cut(s)	337, 590, 626, 808, 887, 1242, 1248, 1489, 1505
Hin6I	GCGC	5 cut(s)	385, 584, 1242, 1399, 1734
HinP1I	GCGC	5 cut(s)	385, 584, 1242, 1399, 1734
HincII	GTYRAC	2 cut(s)	442, 1714
HindII	GTYRAC	2 cut(s)	442, 1714
HindIII	AAGCTT	1 cut(s)	1315
HpaI	GTTAAC	1 cut(s)	1714
HpaII	CCGG	4 cut(s)	48, 966, 1183, 1805
HphI	GGTGA	8 cut(s)	61, 62, 505, 697, 716, 1399, 1609, 1718
Hpy166II	GTNNAC	3 cut(s)	442, 1714, 1802
Hpy188I	TCNGA	6 cut(s)	109, 139, 847, 1117, 1179, 1842
Hpy188III	TCNNGA	9 cut(s)	56, 623, 811, 966, 1197, 1220, 1227, 1325, 1630
Hpy8I	GTNNAC	3 cut(s)	442, 1714, 1802
Hpy99I	CGWCG	1 cut(s)	446
HpyCH4III	ACNGT	6 cut(s)	7, 247, 428, 466, 559, 1539
HpyCH4IV	ACGT	2 cut(s)	444, 1329
HpyCH4V	TGCA	9 cut(s)	26, 207, 314, 722, 1016, 1333, 1445, 1752, 1826
HpyF10VI	GCNNNNNNNGC	2 cut(s)	387, 1418
HpyF3I	CTNAG	9 cut(s)	64, 485, 606, 641, 695, 726, 745, 762, 1717
HpySE526I	ACGT	2 cut(s)	444, 1329
Hsp92II	CATG	9 cut(s)	337, 590, 626, 808, 887, 1242, 1248, 1489, 1505
HspAI	GCGC	5 cut(s)	385, 584, 1242, 1399, 1734
KflI	GGGWCCC	1 cut(s)	1249
Kpn2I	TCCGGA	1 cut(s)	965
Ksp22I	TGATCA	2 cut(s)	402, 495
KspAI	GTTAAC	1 cut(s)	1714
LguI	GCTCTTC	1 cut(s)	323
LmnI	GCTCC	2 cut(s)	1503, 1550
Lsp1109I	GCAGC	3 cut(s)	493, 734, 892
LweI	GCATC	9 cut(s)	237, 301, 1054, 1108, 1202, 1302, 1421, 1432, 1813
MabI	ACCWGGT	1 cut(s)	1686
MaeI	CTAG	9 cut(s)	261, 276, 572, 912, 1340, 1494, 1739, 1763, 1873
MaeII	ACGT	2 cut(s)	444, 1329
MaeIII	GTNAC	9 cut(s)	7, 67, 350, 478, 784, 1096, 1405, 1682, 1868
MbiI	CCGCTC	1 cut(s)	474
MboII	GAAGA	9 cut(s)	223, 299, 340, 404, 680, 1052, 1208, 1529, 1615
MfeI	CAATTG	2 cut(s)	1446, 1529
MflI	RGATCY	2 cut(s)	712, 1830
MhlI	GDGCHC	2 cut(s)	666, 1780
MlsI	TGGCCA	1 cut(s)	1310
MluCI	AATT	6 cut(s)	114, 119, 1446, 1529, 1698, 1849
MluNI	TGGCCA	1 cut(s)	1310
MlyI	GAGTC	4 cut(s)	340, 475, 661, 750
MnlI	CCTC	9 cut(s)	277, 431, 496, 653, 807, 964, 1373, 1626, 1702
Mox20I	TGGCCA	1 cut(s)	1310
MroI	TCCGGA	1 cut(s)	965
MscI	TGGCCA	1 cut(s)	1310
MseI	TTAA	9 cut(s)	29, 297, 435, 717, 867, 1542, 1638, 1701, 1713
MslI	CAYNNNNRTG	4 cut(s)	338, 544, 627, 1243
Msp20I	TGGCCA	1 cut(s)	1310
MspCI	CTTAAG	1 cut(s)	866
MspI	CCGG	4 cut(s)	48, 966, 1183, 1805
MspR9I	CCNGG	4 cut(s)	92, 657, 1688, 1806
MunI	CAATTG	2 cut(s)	1446, 1529
Mva1269I	GAATGC	1 cut(s)	363
MvaI	CCWGG	3 cut(s)	92, 657, 1688
MvnI	CGCG	2 cut(s)	385, 387
MwoI	GCNNNNNNNGC	2 cut(s)	387, 1418
NciI	CCSGG	1 cut(s)	1806
NlaIII	CATG	9 cut(s)	337, 590, 626, 808, 887, 1242, 1248, 1489, 1505
NlaIV	GGNNCC	5 cut(s)	664, 1250, 1251, 1482, 1499
NmuCI	GTSAC	6 cut(s)	7, 67, 478, 1096, 1405, 1682
NsbI	TGCGCA	2 cut(s)	585, 1243
NspI	RCATGY	1 cut(s)	1242
PaeI	GCATGC	1 cut(s)	1242
PagI	TCATGA	1 cut(s)	622
PaqCI	CACCTGC	1 cut(s)	481
PciSI	GCTCTTC	1 cut(s)	323
PctI	GAATGC	1 cut(s)	363
PfeI	GAWTC	8 cut(s)	95, 140, 842, 1052, 1223, 1230, 1389, 1843
PflFI	GACNNNGTC	1 cut(s)	1688
PfoI	TCCNGGA	2 cut(s)	90, 655
PkrI	GCNGC	5 cut(s)	389, 476, 508, 724, 882
Ple19I	CGATCG	1 cut(s)	241
PleI	GAGTC	4 cut(s)	340, 475, 660, 749
PpsI	GAGTC	4 cut(s)	340, 475, 660, 749
PpuMI	RGGWCCY	4 cut(s)	88, 1249, 1480, 1690
PshBI	ATTAAT	1 cut(s)	29
PsiI	TTATAA	1 cut(s)	1028
Psp5II	RGGWCCY	4 cut(s)	88, 1249, 1480, 1690
Psp6I	CCWGG	3 cut(s)	90, 655, 1686
PspGI	CCWGG	3 cut(s)	90, 655, 1686
PspN4I	GGNNCC	5 cut(s)	664, 1250, 1251, 1482, 1499
PspOMI	GGGCCC	1 cut(s)	662
PspPI	GGNCC	9 cut(s)	88, 155, 169, 662, 663, 816, 1249, 1480, 1690
PspPPI	RGGWCCY	4 cut(s)	88, 1249, 1480, 1690
PsuI	RGATCY	2 cut(s)	712, 1830
PsyI	GACNNNGTC	1 cut(s)	1688
PvuI	CGATCG	1 cut(s)	241
RsaI	GTAC	4 cut(s)	280, 1547, 1610, 1837
RsaNI	GTAC	4 cut(s)	279, 1546, 1609, 1836
RseI	CAYNNNNRTG	4 cut(s)	338, 544, 627, 1243
SalI	GTCGAC	1 cut(s)	440
SapI	GCTCTTC	1 cut(s)	323
SaqAI	TTAA	9 cut(s)	29, 297, 435, 717, 867, 1542, 1638, 1701, 1713
SatI	GCNGC	5 cut(s)	388, 475, 507, 723, 881
Sau96I	GGNCC	9 cut(s)	88, 155, 169, 662, 663, 816, 1249, 1480, 1690
SchI	GAGTC	4 cut(s)	340, 475, 661, 750
ScrFI	CCNGG	4 cut(s)	92, 657, 1688, 1806
SduI	GDGCHC	2 cut(s)	666, 1780
SexAI	ACCWGGT	1 cut(s)	1686
SfaNI	GCATC	9 cut(s)	237, 301, 1054, 1108, 1202, 1302, 1421, 1432, 1813
SfcI	CTRYAG	1 cut(s)	1798
SinI	GGWCC	6 cut(s)	88, 169, 816, 1249, 1480, 1690
SmiMI	CAYNNNNRTG	4 cut(s)	338, 544, 627, 1243
SmlI	CTYRAG	3 cut(s)	866, 1630, 1669
SmoI	CTYRAG	3 cut(s)	866, 1630, 1669
SpeI	ACTAGT	1 cut(s)	260
SphI	GCATGC	1 cut(s)	1242
Sse9I	AATT	6 cut(s)	114, 119, 1446, 1529, 1698, 1849
SsiI	CCGC	7 cut(s)	72, 149, 383, 387, 474, 893, 1410
SspMI	CTAG	9 cut(s)	261, 276, 572, 912, 1340, 1494, 1739, 1763, 1873
StyD4I	CCNGG	4 cut(s)	90, 655, 1686, 1804
StyI	CCWWGG	6 cut(s)	510, 1253, 1305, 1426, 1493, 1693
TaaI	ACNGT	6 cut(s)	7, 247, 428, 466, 559, 1539
TaiI	ACGT	2 cut(s)	447, 1332
TaqI	TCGA	6 cut(s)	241, 441, 934, 997, 1455, 1853
TasI	AATT	6 cut(s)	114, 119, 1446, 1529, 1698, 1849
TatI	WGTACW	2 cut(s)	1608, 1835
TauI	GCSGC	2 cut(s)	390, 477
TfiI	GAWTC	8 cut(s)	95, 140, 842, 1052, 1223, 1230, 1389, 1843
Tru1I	TTAA	9 cut(s)	29, 297, 435, 717, 867, 1542, 1638, 1701, 1713
Tru9I	TTAA	9 cut(s)	29, 297, 435, 717, 867, 1542, 1638, 1701, 1713
TscAI	CASTG	4 cut(s)	471, 505, 1023, 1542
TseFI	GTSAC	6 cut(s)	7, 67, 478, 1096, 1405, 1682
TseI	GCWGC	3 cut(s)	506, 722, 880
Tsp45I	GTSAC	6 cut(s)	7, 67, 478, 1096, 1405, 1682
TspGWI	ACGGA	2 cut(s)	296, 1563
TspRI	CASTG	4 cut(s)	471, 505, 1023, 1542
Tth111I	GACNNNGTC	1 cut(s)	1688
Vha464I	CTTAAG	1 cut(s)	866
VpaK11BI	GGWCC	6 cut(s)	88, 169, 816, 1249, 1480, 1690
VspI	ATTAAT	1 cut(s)	29
XceI	RCATGY	1 cut(s)	1242
XcmI	CCANNNNNNNNNTGG	1 cut(s)	517
XmaJI	CCTAGG	1 cut(s)	1493
XmiI	GTMKAC	2 cut(s)	441, 1801
XspI	CTAG	9 cut(s)	261, 276, 572, 912, 1340, 1494, 1739, 1763, 1873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.