MD01G1133800.v1.1

ENTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003209
Physical Location & Seq
Reverse (-)
24355520 .. 24356955
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1133800.v1.1.491

Sequence Viewer

Length: 855 bp
ATGCACGTCGATGGTTCCGACTTCCAGACACGAAAAATAGAAAACATGAGCAGTCCCTTGTTCTATGAACTGAAGAAACAAGCATCTTCCTTCTTGAAAGAGAAGATGAAAACCGCTCGTTTGGCGCTGACTGATGCAACGCCTGCAGAACTAATGACAGAAGAAGCCACAAATGAAAATCCATGGCCACCAGACACTCGTAGCATTGGAGTAATATCAAGGGCTGCCTTTGAAGTTGATGATTACTGCAGGATTGTGGACATTCTACGTAAGAGGCTGTTGAACTTCAATAGAAAGAATTGGCGAGGTTCTTACAAGGCTCTAATTTTATTGGAACACCTTTTATGCCATGGACCATTGAGAATTGCTGGAGAGTTTGAGGACGACAAAGATCTGATCAAGGAGATGGAAAGGTTTCAATATATTGATGAGAAAGGGTTTAACTGGGGGTTGAGCCTCAGGAAGTTATCGAAGAGAGTACTAAAACTTGTCGAAGATGAGGTGTTTTTCAGGGAAGAGAGGGCCAGAGCTCGGAATCTAACGCGTGGAATCGAAGGGTTCGGGAGCCTAAGCCTGCAGCGTTCTTCTTTCATAGATTCAAGCTTGAAGGGCTCGTCTCTTAAAACATATGAGAGAAGTAATTCTCACTACAACGATCATCAAAGTCGCGAAAGCCAAGTCTTTGGCTCGAACAAGAAGTTTGTAATGAAAGAAGAGATGGAGAAACCGCAGCAGAACGATGATCACGACAACATCTCAGAACCTGAAGTGAATCTTGACGTAGGGGATCACCCATTTTGCCAGGATGAGCATCAGACGGCAGAGTCCCTTCTTTCTACTGTAAATGAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ENTH

Protein Analysis

285

Amino Acids

32.93

Weight (kDa)

5.72

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENTH PF01417 47 - 167 2e-30 ENTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 823
AccBSI CCGCTC 1 cut(s) 116
AccII CGCG 2 cut(s) 544, 669
AciI CCGC 2 cut(s) 114, 728
AclWI GGATC 1 cut(s) 795
AcoI YGGCCR 1 cut(s) 185
AcuI CTGAAG 2 cut(s) 92, 786
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 531
AflIII ACRYGT 1 cut(s) 542
AgsI TTSAA 7 cut(s) 97, 233, 283, 289, 419, 600, 607
AjiI CACGTC 1 cut(s) 7
AjnI CCWGG 1 cut(s) 801
AluBI AGCT 2 cut(s) 530, 603
AluI AGCT 2 cut(s) 530, 603
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 1 cut(s) 621
AlwI GGATC 1 cut(s) 795
AlwNI CAGNNNCTG 1 cut(s) 764
AoxI GGCC 2 cut(s) 185, 522
ApeKI GCWGC 3 cut(s) 224, 577, 730
ArsI GACNNNNNNTTYG 2 cut(s) 663, 695
Asp700I GAANNNNTTC 2 cut(s) 414, 640
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 2 cut(s) 353, 522
AsuHPI GGTGA 1 cut(s) 782
AvaII GGWCC 1 cut(s) 353
AxyI CCTNAGG 1 cut(s) 458
BalI TGGCCA 1 cut(s) 187
BanII GRGCYC 2 cut(s) 532, 614
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 3 cut(s) 211, 589, 742
BccI CCATC 3 cut(s) 5, 400, 712
BceAI ACGGC 1 cut(s) 834
BciT130I CCWGG 1 cut(s) 803
BclI TGATCA 2 cut(s) 396, 742
BcoDI GTCTC 1 cut(s) 621
BfmI CTRYAG 3 cut(s) 144, 247, 575
BfoI RGCGCY 1 cut(s) 128
BglII AGATCT 1 cut(s) 391
BisI GCNGC 3 cut(s) 225, 578, 731
BlsI GCNGC 3 cut(s) 226, 579, 732
BmcAI AGTACT 1 cut(s) 480
Bme1390I CCNGG 1 cut(s) 803
Bme18I GGWCC 1 cut(s) 353
BmgBI CACGTC 1 cut(s) 7
BmgT120I GGNCC 2 cut(s) 353, 522
BmiI GGNNCC 2 cut(s) 16, 566
BmrFI CCNGG 1 cut(s) 803
BmrI ACTGGG 1 cut(s) 454
BmsI GCATC 3 cut(s) 92, 124, 820
BmuI ACTGGG 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 390
Bpu10I CCTNAGC 1 cut(s) 569
BsaAI YACGTR 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 810
BsaJI CCNNGG 2 cut(s) 182, 349
Bsc4I CCNNNNNNNGG 1 cut(s) 531
Bse1I ACTGG 1 cut(s) 449
Bse21I CCTNAGG 1 cut(s) 458
Bse8I GATNNNNATC 1 cut(s) 810
BseBI CCWGG 1 cut(s) 803
BseDI CCNNGG 2 cut(s) 182, 349
BseGI GGATG 1 cut(s) 811
BseJI GATNNNNATC 1 cut(s) 810
BseLI CCNNNNNNNGG 1 cut(s) 531
BseMII CTCAG 2 cut(s) 472, 771
BseNI ACTGG 1 cut(s) 449
BseXI GCAGC 3 cut(s) 211, 589, 742
Bsh1236I CGCG 2 cut(s) 544, 669
BshFI GGCC 2 cut(s) 187, 524
BsiHKAI GWGCWC 1 cut(s) 532
BslFI GGGAC 2 cut(s) 39, 811
BslI CCNNNNNNNGG 1 cut(s) 531
BsmAI GTCTC 1 cut(s) 621
BsmBI CGTCTC 1 cut(s) 621
BsmFI GGGAC 2 cut(s) 39, 811
BsnI GGCC 2 cut(s) 187, 524
Bsp1286I GDGCHC 2 cut(s) 532, 614
Bsp143I GATC 5 cut(s) 391, 396, 655, 742, 787
Bsp19I CCATGG 2 cut(s) 182, 349
Bsp68I TCGCGA 1 cut(s) 669
BspACI CCGC 2 cut(s) 114, 728
BspANI GGCC 2 cut(s) 187, 524
BspCNI CTCAG 2 cut(s) 471, 770
BspFNI CGCG 2 cut(s) 544, 669
BspLI GGNNCC 2 cut(s) 16, 566
BspMAI CTGCAG 3 cut(s) 148, 251, 579
BspPI GGATC 1 cut(s) 795
BsrBI CCGCTC 1 cut(s) 116
BsrI ACTGG 1 cut(s) 449
BssECI CCNNGG 2 cut(s) 182, 349
BssMI GATC 5 cut(s) 391, 396, 655, 742, 787
BssT1I CCWWGG 2 cut(s) 182, 349
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 1 cut(s) 841
Bst6I CTCTTC 3 cut(s) 467, 510, 708
BstAPI GCANNNNNTGC 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 144, 575
BstDEI CTNAG 3 cut(s) 458, 569, 757
BstDSI CCRYGG 2 cut(s) 182, 349
BstF5I GGATG 1 cut(s) 811
BstFNI CGCG 2 cut(s) 544, 669
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 5 cut(s) 394, 399, 658, 745, 790
BstMAI GTCTC 1 cut(s) 621
BstMBI GATC 5 cut(s) 391, 396, 655, 742, 787
BstMWI GCNNNNNNNGC 3 cut(s) 122, 143, 609
BstNI CCWGG 1 cut(s) 803
BstSCI CCNGG 1 cut(s) 801
BstSFI CTRYAG 3 cut(s) 144, 247, 575
BstSNI TACGTA 1 cut(s) 269
BstUI CGCG 2 cut(s) 544, 669
BstV1I GCAGC 3 cut(s) 211, 589, 742
BstX2I RGATCY 1 cut(s) 391
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 1 cut(s) 391
Bsu36I CCTNAGG 1 cut(s) 458
BsuRI GGCC 2 cut(s) 187, 524
BtgI CCRYGG 2 cut(s) 182, 349
BtrI CACGTC 1 cut(s) 7
BtsCI GGATG 1 cut(s) 811
BtuMI TCGCGA 1 cut(s) 669
Cac8I GCNNGC 2 cut(s) 144, 575
CaiI CAGNNNCTG 1 cut(s) 764
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 2 cut(s) 353, 522
Csp6I GTAC 1 cut(s) 479
CviAII CATG 3 cut(s) 46, 183, 350
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 3 cut(s) 458, 569, 757
DpnI GATC 5 cut(s) 393, 398, 657, 744, 789
DpnII GATC 5 cut(s) 391, 396, 655, 742, 787
DrdI GACNNNNNNGTC 1 cut(s) 823
DseDI GACNNNNNNGTC 1 cut(s) 823
EaeI YGGCCR 1 cut(s) 185
Eam1104I CTCTTC 3 cut(s) 467, 510, 708
EarI CTCTTC 3 cut(s) 467, 510, 708
Ecl136II GAGCTC 1 cut(s) 530
Eco105I TACGTA 1 cut(s) 269
Eco130I CCWWGG 2 cut(s) 182, 349
Eco24I GRGCYC 2 cut(s) 532, 614
Eco47I GGWCC 1 cut(s) 353
Eco53kI GAGCTC 1 cut(s) 530
Eco57I CTGAAG 2 cut(s) 92, 786
Eco81I CCTNAGG 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 530
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 2 cut(s) 182, 349
EcoT38I GRGCYC 2 cut(s) 532, 614
ErhI CCWWGG 2 cut(s) 182, 349
Esp3I CGTCTC 1 cut(s) 621
FaeI CATG 3 cut(s) 49, 186, 353
FaiI YATR 9 cut(s) 47, 66, 184, 346, 351, 423, 593, 628, 630
FalI AAGNNNNNCTT 2 cut(s) 759, 791
FaqI GGGAC 2 cut(s) 39, 811
FatI CATG 3 cut(s) 45, 182, 349
FauNDI CATATG 1 cut(s) 628
FbaI TGATCA 2 cut(s) 396, 742
Fnu4HI GCNGC 3 cut(s) 225, 578, 731
FokI GGATG 1 cut(s) 818
FriOI GRGCYC 2 cut(s) 532, 614
Fsp4HI GCNGC 3 cut(s) 225, 578, 731
GlaI GCGC 1 cut(s) 126
GluI GCNGC 3 cut(s) 225, 578, 731
GsuI CTGGAG 1 cut(s) 390
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 2 cut(s) 187, 524
HhaI GCGC 1 cut(s) 127
Hin1II CATG 3 cut(s) 49, 186, 353
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HindIII AAGCTT 1 cut(s) 601
HinfI GANTC 6 cut(s) 535, 549, 596, 772, 824, 848
HphI GGTGA 1 cut(s) 782
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 5 cut(s) 19, 396, 534, 760, 816
Hpy188III TCNNGA 8 cut(s) 25, 94, 460, 562, 668, 746, 776, 852
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 100, 548, 601, 839
HpyCH4III ACNGT 1 cut(s) 841
HpyCH4IV ACGT 3 cut(s) 6, 268, 780
HpyCH4V TGCA 5 cut(s) 4, 137, 146, 249, 577
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 143, 609
HpyF3I CTNAG 3 cut(s) 458, 569, 757
HpySE526I ACGT 3 cut(s) 6, 268, 780
Hsp92II CATG 3 cut(s) 49, 186, 353
HspAI GCGC 1 cut(s) 125
Ksp22I TGATCA 2 cut(s) 396, 742
Kzo9I GATC 5 cut(s) 391, 396, 655, 742, 787
LmnI GCTCC 1 cut(s) 564
Lsp1109I GCAGC 3 cut(s) 211, 589, 742
LweI GCATC 3 cut(s) 92, 124, 820
MaeII ACGT 3 cut(s) 6, 268, 780
MalI GATC 5 cut(s) 393, 398, 657, 744, 789
MbiI CCGCTC 1 cut(s) 116
MboI GATC 5 cut(s) 391, 396, 655, 742, 787
MboII GAAGA 9 cut(s) 78, 85, 115, 173, 484, 506, 527, 576, 725
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 2 cut(s) 532, 614
MlsI TGGCCA 1 cut(s) 187
MluCI AATT 4 cut(s) 298, 324, 363, 640
MluI ACGCGT 1 cut(s) 542
MluNI TGGCCA 1 cut(s) 187
MlyI GAGTC 1 cut(s) 833
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 6 cut(s) 267, 299, 373, 467, 493, 513
Mox20I TGGCCA 1 cut(s) 187
MroXI GAANNNNTTC 2 cut(s) 414, 640
MscI TGGCCA 1 cut(s) 187
MseI TTAA 2 cut(s) 441, 621
MslI CAYNNNNRTG 1 cut(s) 9
Msp20I TGGCCA 1 cut(s) 187
MspR9I CCNGG 1 cut(s) 803
MvaI CCWGG 1 cut(s) 803
MvnI CGCG 2 cut(s) 544, 669
MwoI GCNNNNNNNGC 3 cut(s) 122, 143, 609
NcoI CCATGG 2 cut(s) 182, 349
NdeI CATATG 1 cut(s) 628
NdeII GATC 5 cut(s) 391, 396, 655, 742, 787
NlaIII CATG 3 cut(s) 49, 186, 353
NlaIV GGNNCC 2 cut(s) 16, 566
NruI TCGCGA 1 cut(s) 669
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 744
PdmI GAANNNNTTC 2 cut(s) 414, 640
PfeI GAWTC 5 cut(s) 535, 549, 596, 772, 848
PkrI GCNGC 3 cut(s) 226, 579, 732
PleI GAGTC 1 cut(s) 832
PpsI GAGTC 1 cut(s) 832
Ppu21I YACGTR 1 cut(s) 269
Psp124BI GAGCTC 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 2 cut(s) 16, 566
PspPI GGNCC 2 cut(s) 353, 522
PstI CTGCAG 3 cut(s) 148, 251, 579
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 1 cut(s) 391
RruI TCGCGA 1 cut(s) 669
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
RseI CAYNNNNRTG 1 cut(s) 9
SacI GAGCTC 1 cut(s) 532
SaqAI TTAA 2 cut(s) 441, 621
SatI GCNGC 3 cut(s) 225, 578, 731
Sau3AI GATC 5 cut(s) 391, 396, 655, 742, 787
Sau96I GGNCC 2 cut(s) 353, 522
ScaI AGTACT 1 cut(s) 480
SchI GAGTC 1 cut(s) 833
ScrFI CCNGG 1 cut(s) 803
SduI GDGCHC 2 cut(s) 532, 614
SfaNI GCATC 3 cut(s) 92, 124, 820
SfcI CTRYAG 3 cut(s) 144, 247, 575
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 1 cut(s) 9
SnaBI TACGTA 1 cut(s) 269
Sse9I AATT 4 cut(s) 298, 324, 363, 640
SsiI CCGC 2 cut(s) 114, 728
SstI GAGCTC 1 cut(s) 532
StyD4I CCNGG 1 cut(s) 801
StyI CCWWGG 2 cut(s) 182, 349
TaaI ACNGT 1 cut(s) 841
TaiI ACGT 3 cut(s) 9, 271, 783
TaqI TCGA 5 cut(s) 9, 470, 492, 552, 689
TasI AATT 4 cut(s) 298, 324, 363, 640
TatI WGTACW 1 cut(s) 478
TfiI GAWTC 5 cut(s) 535, 549, 596, 772, 848
Tru1I TTAA 2 cut(s) 441, 621
Tru9I TTAA 2 cut(s) 441, 621
TseI GCWGC 3 cut(s) 224, 577, 730
TspDTI ATGAA 5 cut(s) 81, 122, 189, 580, 722
VpaK11BI GGWCC 1 cut(s) 353
XmnI GAANNNNTTC 2 cut(s) 414, 640
ZrmI AGTACT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.