MD03G1095100.v1.1

ENTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003209
Physical Location & Seq
Forward (+)
8067586 .. 8069661
2076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1095100.v1.1.491

Sequence Viewer

Length: 966 bp
ATGTCGATCTTAAGCAACAATAGCAGCAGCAACGATGGAGCACCTTCATTTCATGAGTTCAAGAAACAAGCTTCTTTCTTCTTCAAGGAGAAGATCAAGACTGCAAGACTAGTCCTCACAGATGTTACACCAGCGCAATTATTGACTGAAGAAGCTACAGATGGGAATCCATTGTCACCAGACACAAGGACCTTGGGTTCAATTTCAAAGGCTGCTTTTGAACTGGATGATTACACAAGAATTGTGGAGATTTTGCACAAAAGATTAGAGAAGTTTGAGAGAAAGAACTGGAGGGTATCCTACAATTCTCTGATTGTAGTTGAGCATTTACTTACTCATGGACCGGAAAGTGTTGCAGTTGAGTTTGAGATTGACAAAGATGTTATTGAAGAGATGGGAAGCTTTCAGCATATAGATGAGAAAGGATTCAATTGGGGCCTTGCTCTTAGAAAGAAGTCGGGGAGAATTTTGAAACTGCTAGAGAAAGGGCCTCTTCTCAAAGAGGAGAGAGACAGAGCTAGGAAGCTAACAAGAGGGATCCAAGGGTTTGGAAGCTTCTGCCAAAGATCATCTGGTTCTTCAGCACAAGGGATTCTTGGAGTTCAAACGTATGGAAGGTCTAATTCTCAATTTCTCTCCAGTGACGATGAAAACCAAGAGAATCAGTTTCCATCATCTGACGTCGAAGATTTTACTAAGAAAGTTGAAGAATCAAAGCAGAGCAACGGAAGCATTGCGATCATTGAGGCCGTCAAGGACGAAGCAGAGAAACCCGAAACTTCAAGTTTTAAAGAAAGCTTGGTTCCAGAGAAGGAAGAGTTAGAGAGTTGGAACTGCAAGGGAGAGTCTAACCCACTTTTGGGTGGTGAGAAAGATGTGTCTAGGTTTGAAGTTTCTGCTGAGAATGAACACCCCTTCAATGAAACACACAATCCAAGTGCTGCTTCATTGCTTGCTGCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ENTH

Protein Analysis

322

Amino Acids

35.84

Weight (kDa)

5.19

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENTH PF01417 43 - 163 4.2e-28 ENTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 684
AclWI GGATC 2 cut(s) 532, 545
AcsI RAATTY 1 cut(s) 465
AcuI CTGAAG 2 cut(s) 168, 564
AcyI GRCGYC 1 cut(s) 681
AfiI CCNNNNNNNGG 2 cut(s) 859, 860
AflII CTTAAG 1 cut(s) 10
AhlI ACTAGT 1 cut(s) 109
AjuI GAANNNNNNNTTGG 2 cut(s) 928, 960
AloI GAACNNNNNNTCC 2 cut(s) 181, 213
AluBI AGCT 7 cut(s) 71, 155, 402, 518, 526, 555, 798
AluI AGCT 7 cut(s) 71, 155, 402, 518, 526, 555, 798
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 2 cut(s) 532, 545
AoxI GGCC 3 cut(s) 436, 488, 747
ApeKI GCWGC 5 cut(s) 24, 27, 212, 941, 956
ApoI RAATTY 1 cut(s) 465
Asp700I GAANNNNTTC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 4 cut(s) 189, 341, 436, 488
AsuHPI GGTGA 2 cut(s) 168, 878
AvaII GGWCC 2 cut(s) 189, 341
BamHI GGATCC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 5 cut(s) 36, 39, 199, 928, 943
BccI CCATC 4 cut(s) 29, 155, 388, 679
BceAI ACGGC 1 cut(s) 734
BciVI GTATCC 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 504
BcuI ACTAGT 1 cut(s) 109
BfaI CTAG 4 cut(s) 110, 479, 519, 882
BfmI CTRYAG 1 cut(s) 156
BfrI CTTAAG 1 cut(s) 10
BfuI GTATCC 1 cut(s) 307
BisI GCNGC 5 cut(s) 25, 28, 213, 942, 957
BlsI GCNGC 5 cut(s) 26, 29, 214, 943, 958
Bme18I GGWCC 2 cut(s) 189, 341
BmgT120I GGNCC 4 cut(s) 189, 341, 436, 488
BmiI GGNNCC 3 cut(s) 437, 539, 804
BpmI CTGGAG 2 cut(s) 310, 622
BsaBI GATNNNNATC 1 cut(s) 165
BsaHI GRCGYC 1 cut(s) 681
BsaJI CCNNGG 2 cut(s) 192, 541
BsaWI WCCGGW 1 cut(s) 343
Bsc4I CCNNNNNNNGG 2 cut(s) 859, 860
Bse1I ACTGG 3 cut(s) 228, 293, 639
Bse3DI GCAATG 2 cut(s) 732, 947
Bse8I GATNNNNATC 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 192, 541
BseGI GGATG 1 cut(s) 232
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 859, 860
BseMI GCAATG 2 cut(s) 732, 947
BseMII CTCAG 1 cut(s) 891
BseNI ACTGG 3 cut(s) 228, 293, 639
BseRI GAGGAG 1 cut(s) 518
BseXI GCAGC 5 cut(s) 36, 39, 199, 928, 943
BshFI GGCC 3 cut(s) 438, 490, 749
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 1 cut(s) 344
BslI CCNNNNNNNGG 2 cut(s) 859, 860
BsmAI GTCTC 1 cut(s) 504
BsnI GGCC 3 cut(s) 438, 490, 749
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 5 cut(s) 6, 93, 537, 566, 738
BspANI GGCC 3 cut(s) 438, 490, 749
BspCNI CTCAG 1 cut(s) 892
BspHI TCATGA 1 cut(s) 52
BspLI GGNNCC 3 cut(s) 437, 539, 804
BspPI GGATC 2 cut(s) 532, 545
BspTI CTTAAG 1 cut(s) 10
BsrDI GCAATG 2 cut(s) 732, 947
BsrI ACTGG 3 cut(s) 228, 293, 639
BssECI CCNNGG 2 cut(s) 192, 541
BssMI GATC 5 cut(s) 6, 93, 537, 566, 738
BssNI GRCGYC 1 cut(s) 681
BssT1I CCWWGG 2 cut(s) 192, 541
Bst6I CTCTTC 3 cut(s) 384, 498, 810
BstACI GRCGYC 1 cut(s) 681
BstAFI CTTAAG 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 954
BstDEI CTNAG 3 cut(s) 446, 696, 900
BstF5I GGATG 1 cut(s) 232
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 5 cut(s) 9, 96, 540, 569, 741
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 5 cut(s) 6, 93, 537, 566, 738
BstMWI GCNNNNNNNGC 2 cut(s) 21, 729
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 5 cut(s) 36, 39, 199, 928, 943
BstX2I RGATCY 1 cut(s) 537
BstXI CCANNNNNNTGG 1 cut(s) 548
BstYI RGATCY 1 cut(s) 537
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 3 cut(s) 438, 490, 749
BtsCI GGATG 1 cut(s) 232
BtsIMutI CAGTG 1 cut(s) 646
Cac8I GCNNGC 1 cut(s) 954
CciI TCATGA 1 cut(s) 52
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 4 cut(s) 189, 341, 436, 488
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviAII CATG 2 cut(s) 53, 338
DdeI CTNAG 3 cut(s) 446, 696, 900
DpnI GATC 5 cut(s) 8, 95, 539, 568, 740
DpnII GATC 5 cut(s) 6, 93, 537, 566, 738
DraI TTTAAA 1 cut(s) 790
Eam1104I CTCTTC 3 cut(s) 384, 498, 810
EarI CTCTTC 3 cut(s) 384, 498, 810
Eco130I CCWWGG 2 cut(s) 192, 541
Eco47I GGWCC 2 cut(s) 189, 341
Eco57I CTGAAG 2 cut(s) 168, 564
EcoO109I RGGNCCY 3 cut(s) 189, 436, 488
EcoT14I CCWWGG 2 cut(s) 192, 541
ErhI CCWWGG 2 cut(s) 192, 541
FaeI CATG 2 cut(s) 56, 341
FaiI YATR 5 cut(s) 54, 339, 411, 413, 612
FalI AAGNNNNNCTT 6 cut(s) 477, 509, 579, 611, 928, 960
FatI CATG 2 cut(s) 52, 337
Fnu4HI GCNGC 5 cut(s) 25, 28, 213, 942, 957
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 5 cut(s) 25, 28, 213, 942, 957
FspBI CTAG 4 cut(s) 110, 479, 519, 882
GlaI GCGC 1 cut(s) 135
GluI GCNGC 5 cut(s) 25, 28, 213, 942, 957
GsuI CTGGAG 2 cut(s) 310, 622
HaeIII GGCC 3 cut(s) 438, 490, 749
HapII CCGG 1 cut(s) 344
HhaI GCGC 1 cut(s) 136
Hin1I GRCGYC 1 cut(s) 681
Hin1II CATG 2 cut(s) 56, 341
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HindIII AAGCTT 4 cut(s) 69, 400, 553, 796
HinfI GANTC 6 cut(s) 166, 426, 592, 661, 710, 845
HpaII CCGG 1 cut(s) 344
HphI GGTGA 2 cut(s) 168, 878
Hpy188I TCNGA 2 cut(s) 312, 679
Hpy188III TCNNGA 4 cut(s) 53, 61, 97, 806
Hpy99I CGWCG 1 cut(s) 686
HpyAV CCTTC 4 cut(s) 54, 609, 805, 925
HpyCH4IV ACGT 2 cut(s) 608, 681
HpyCH4V TGCA 5 cut(s) 104, 256, 356, 837, 959
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 729
HpyF3I CTNAG 3 cut(s) 446, 696, 900
HpySE526I ACGT 2 cut(s) 608, 681
Hsp92I GRCGYC 1 cut(s) 681
Hsp92II CATG 2 cut(s) 56, 341
HspAI GCGC 1 cut(s) 134
Kzo9I GATC 5 cut(s) 6, 93, 537, 566, 738
LmnI GCTCC 1 cut(s) 38
LpnPI CCDG 8 cut(s) 144, 192, 209, 274, 357, 558, 652, 819
Lsp1109I GCAGC 5 cut(s) 36, 39, 199, 928, 943
MaeI CTAG 4 cut(s) 110, 479, 519, 882
MaeII ACGT 2 cut(s) 608, 681
MaeIII GTNAC 3 cut(s) 124, 174, 641
MalI GATC 5 cut(s) 8, 95, 539, 568, 740
MboI GATC 5 cut(s) 6, 93, 537, 566, 738
MfeI CAATTG 1 cut(s) 430
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 8 cut(s) 137, 201, 240, 304, 430, 465, 622, 629
MlyI GAGTC 1 cut(s) 854
MmeI TCCRAC 1 cut(s) 809
MnlI CCTC 6 cut(s) 125, 285, 496, 501, 527, 739
MroXI GAANNNNTTC 1 cut(s) 425
MseI TTAA 3 cut(s) 11, 789, 964
MslI CAYNNNNRTG 1 cut(s) 414
MspCI CTTAAG 1 cut(s) 10
MspI CCGG 1 cut(s) 344
MunI CAATTG 1 cut(s) 430
MwoI GCNNNNNNNGC 2 cut(s) 21, 729
NdeII GATC 5 cut(s) 6, 93, 537, 566, 738
NlaIII CATG 2 cut(s) 56, 341
NlaIV GGNNCC 3 cut(s) 437, 539, 804
NmuCI GTSAC 2 cut(s) 174, 641
PagI TCATGA 1 cut(s) 52
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 5 cut(s) 166, 426, 592, 661, 710
PkrI GCNGC 5 cut(s) 26, 29, 214, 943, 958
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
PpuMI RGGWCCY 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 189
PspN4I GGNNCC 3 cut(s) 437, 539, 804
PspPI GGNCC 4 cut(s) 189, 341, 436, 488
PspPPI RGGWCCY 1 cut(s) 189
PsuI RGATCY 1 cut(s) 537
RseI CAYNNNNRTG 1 cut(s) 414
SaqAI TTAA 3 cut(s) 11, 789, 964
SatI GCNGC 5 cut(s) 25, 28, 213, 942, 957
Sau3AI GATC 5 cut(s) 6, 93, 537, 566, 738
Sau96I GGNCC 4 cut(s) 189, 341, 436, 488
SchI GAGTC 1 cut(s) 854
SduI GDGCHC 1 cut(s) 43
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 2 cut(s) 189, 341
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
SpeI ACTAGT 1 cut(s) 109
Sse9I AATT 8 cut(s) 137, 201, 240, 304, 430, 465, 622, 629
SspMI CTAG 4 cut(s) 110, 479, 519, 882
StyI CCWWGG 2 cut(s) 192, 541
TaiI ACGT 2 cut(s) 611, 684
TaqI TCGA 2 cut(s) 5, 684
TasI AATT 8 cut(s) 137, 201, 240, 304, 430, 465, 622, 629
TfiI GAWTC 5 cut(s) 166, 426, 592, 661, 710
Tru1I TTAA 3 cut(s) 11, 789, 964
Tru9I TTAA 3 cut(s) 11, 789, 964
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 2 cut(s) 174, 641
TseI GCWGC 5 cut(s) 24, 27, 212, 941, 956
Tsp45I GTSAC 2 cut(s) 174, 641
TspDTI ATGAA 6 cut(s) 36, 41, 663, 921, 936, 936
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 1 cut(s) 646
Vha464I CTTAAG 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 189, 341
XapI RAATTY 1 cut(s) 465
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 4 cut(s) 110, 479, 519, 882
ZraI GACGTC 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.