MD03G1094900.v1.1

ENTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003209
Physical Location & Seq
Forward (+)
8044863 .. 8046507
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1094900.v1.1.491

Sequence Viewer

Length: 1005 bp
ATGTTGATCTTAAGCAACAATAGCAGCAGCAAGGATGGAGCACGTTCATTTCATGAGTTCAAGAAACAAGCTTCTTTCTTCTTCAAGGAGAAGATCAAGACTGCAAGACTAGTCCTCACAGATGTTACACCAGCGCAATTATTGACTGAAGAAGCTACAAATGGGAATTCATTGTCACCGGACACAAGGACCTTGGGTTCAATTTCAAGGGCTGCTTTTGAACTGGATGATTACACAAGAATTGTGGAGATTTTGCACAAAAGATTAGTGAAGTTTGAAAGAAGGAACTGGAGGGTATCCCACAGTTCTCTGATTGTAGTTGAGCATTTACTTACTCATGGACCGGAAAGTGTTGCAGTTGAGTTTGAGATTGACAAAGATGTTATTGAGCAGATGGGAAGCTTTAAGCATATAGATGAGAAAGGATTCAATTGGGGTCTTGCTCTTAGAAAGAAGTCGGGGAGAATTTTGACGCTGCTAGAGAAAGGGCCTCTTCTCAAAGAGGAGAGAGACAGAGCTAGGAAGCTAACAAGAGGGATCCAAGGGTTTGGAAGCTGCCAAAGATCATCTGGTTCTTCAGCACAAGGAATTCTTGGTGAATCATCATTTCAAACATATGGAAGGTCTAATTCTCAATTTCTCTCCAGCGACGAGGAAAACCAAGAGAATCAGTTCCCATCATCTGACATTGAAGATTTTACTAAGAAAGTTGAAGAATCAAAGCGGAGCAATGAAAGCAACACGATCATTGAGGCCGTCCAGGACAGAGCAGAGAAACCCAAAACTCAAATGAGTTTTAAAGAAAACTTGGTTCCAAAGAAGGAAGAGTTAGAGTGGTGGAACTGCAAGGGAGAGTCTAACCCACTTTTGGGTAGTGAGAAAGATGTGTCTAGATTTGAAGTTTCTGCTGAGAACGAACACCCCTTCAATGAAACACGCAATCTAAGTGCTGCTTCATTGCTTGCTGCAAGTTCATGCACTCTTAAAAAAAAGACCATTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ENTH

Protein Analysis

335

Amino Acids

37.73

Weight (kDa)

7.07

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENTH PF01417 43 - 163 2.6e-26 ENTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 724
AclWI GGATC 2 cut(s) 532, 545
AcsI RAATTY 3 cut(s) 166, 465, 588
AcuI CTGAAG 2 cut(s) 168, 561
AfiI CCNNNNNNNGG 2 cut(s) 868, 869
AflII CTTAAG 1 cut(s) 10
AhlI ACTAGT 1 cut(s) 109
AjnI CCWGG 1 cut(s) 759
AloI GAACNNNNNNTCC 2 cut(s) 181, 213
AluBI AGCT 6 cut(s) 71, 155, 402, 518, 526, 555
AluI AGCT 6 cut(s) 71, 155, 402, 518, 526, 555
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 1 cut(s) 504
AlwI GGATC 2 cut(s) 532, 545
AoxI GGCC 2 cut(s) 488, 753
ApeKI GCWGC 7 cut(s) 24, 27, 212, 475, 555, 950, 965
ApoI RAATTY 3 cut(s) 166, 465, 588
Asp700I GAANNNNTTC 2 cut(s) 425, 671
AspLEI GCGC 1 cut(s) 136
AspS9I GGNCC 3 cut(s) 189, 341, 488
AsuHPI GGTGA 2 cut(s) 168, 608
AvaII GGWCC 2 cut(s) 189, 341
BamHI GGATCC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 7 cut(s) 36, 39, 199, 462, 542, 937, 952
BccI CCATC 3 cut(s) 29, 388, 685
BceAI ACGGC 1 cut(s) 740
BciT130I CCWGG 1 cut(s) 761
BciVI GTATCC 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 504
BcuI ACTAGT 1 cut(s) 109
BfaI CTAG 4 cut(s) 110, 479, 519, 891
BfrI CTTAAG 1 cut(s) 10
BfuI GTATCC 1 cut(s) 307
BisI GCNGC 7 cut(s) 25, 28, 213, 476, 556, 951, 966
BlsI GCNGC 7 cut(s) 26, 29, 214, 477, 557, 952, 967
Bme1390I CCNGG 1 cut(s) 761
Bme18I GGWCC 2 cut(s) 189, 341
BmgT120I GGNCC 3 cut(s) 189, 341, 488
BmiI GGNNCC 2 cut(s) 539, 813
BmrFI CCNGG 1 cut(s) 761
BpmI CTGGAG 2 cut(s) 310, 628
BsaJI CCNNGG 2 cut(s) 192, 541
BsaWI WCCGGW 2 cut(s) 178, 343
Bsc4I CCNNNNNNNGG 2 cut(s) 868, 869
Bse1I ACTGG 2 cut(s) 228, 293
Bse3DI GCAATG 2 cut(s) 736, 956
BseBI CCWGG 1 cut(s) 761
BseDI CCNNGG 2 cut(s) 192, 541
BseGI GGATG 2 cut(s) 40, 232
BseLI CCNNNNNNNGG 2 cut(s) 868, 869
BseMI GCAATG 2 cut(s) 736, 956
BseMII CTCAG 1 cut(s) 900
BseNI ACTGG 2 cut(s) 228, 293
BseRI GAGGAG 1 cut(s) 518
BseXI GCAGC 7 cut(s) 36, 39, 199, 462, 542, 937, 952
BshFI GGCC 2 cut(s) 490, 755
BsiHKAI GWGCWC 1 cut(s) 43
BsiSI CCGG 2 cut(s) 179, 344
BslI CCNNNNNNNGG 2 cut(s) 868, 869
BsmAI GTCTC 1 cut(s) 504
BsnI GGCC 2 cut(s) 490, 755
Bsp1286I GDGCHC 1 cut(s) 43
Bsp143I GATC 5 cut(s) 6, 93, 537, 563, 744
BspACI CCGC 1 cut(s) 724
BspANI GGCC 2 cut(s) 490, 755
BspCNI CTCAG 1 cut(s) 901
BspHI TCATGA 1 cut(s) 52
BspLI GGNNCC 2 cut(s) 539, 813
BspPI GGATC 2 cut(s) 532, 545
BspTI CTTAAG 1 cut(s) 10
BsrDI GCAATG 2 cut(s) 736, 956
BsrI ACTGG 2 cut(s) 228, 293
BssECI CCNNGG 2 cut(s) 192, 541
BssMI GATC 5 cut(s) 6, 93, 537, 563, 744
BssT1I CCWWGG 2 cut(s) 192, 541
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 2 cut(s) 498, 819
BstAFI CTTAAG 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 963
BstDEI CTNAG 4 cut(s) 446, 702, 909, 944
BstF5I GGATG 2 cut(s) 40, 232
BstHHI GCGC 1 cut(s) 136
BstKTI GATC 5 cut(s) 9, 96, 540, 566, 747
BstMAI GTCTC 1 cut(s) 504
BstMBI GATC 5 cut(s) 6, 93, 537, 563, 744
BstMWI GCNNNNNNNGC 2 cut(s) 21, 735
BstNI CCWGG 1 cut(s) 761
BstSCI CCNGG 1 cut(s) 759
BstV1I GCAGC 7 cut(s) 36, 39, 199, 462, 542, 937, 952
BstX2I RGATCY 1 cut(s) 537
BstXI CCANNNNNNTGG 1 cut(s) 548
BstYI RGATCY 1 cut(s) 537
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 2 cut(s) 490, 755
BtsCI GGATG 2 cut(s) 40, 232
Cac8I GCNNGC 1 cut(s) 963
CciI TCATGA 1 cut(s) 52
CfoI GCGC 1 cut(s) 136
Cfr13I GGNCC 3 cut(s) 189, 341, 488
CseI GACGC 1 cut(s) 481
CspCI CAANNNNNGTGG 2 cut(s) 225, 260
CviAII CATG 3 cut(s) 53, 338, 975
CviJI RGCY 9 cut(s) 71, 155, 212, 402, 490, 518, 526, 555, 755
CviKI_1 RGCY 9 cut(s) 71, 155, 212, 402, 490, 518, 526, 555, 755
DdeI CTNAG 4 cut(s) 446, 702, 909, 944
DpnI GATC 5 cut(s) 8, 95, 539, 565, 746
DpnII GATC 5 cut(s) 6, 93, 537, 563, 744
DraI TTTAAA 1 cut(s) 799
Eam1104I CTCTTC 2 cut(s) 498, 819
EarI CTCTTC 2 cut(s) 498, 819
Eco130I CCWWGG 2 cut(s) 192, 541
Eco47I GGWCC 2 cut(s) 189, 341
Eco57I CTGAAG 2 cut(s) 168, 561
EcoO109I RGGNCCY 2 cut(s) 189, 488
EcoRI GAATTC 2 cut(s) 166, 588
EcoRII CCWGG 1 cut(s) 759
EcoT14I CCWWGG 2 cut(s) 192, 541
ErhI CCWWGG 2 cut(s) 192, 541
FaeI CATG 3 cut(s) 56, 341, 978
FaiI YATR 7 cut(s) 54, 339, 411, 413, 616, 618, 976
FalI AAGNNNNNCTT 8 cut(s) 199, 231, 477, 509, 576, 608, 937, 969
FatI CATG 3 cut(s) 52, 337, 974
FauNDI CATATG 1 cut(s) 616
Fnu4HI GCNGC 7 cut(s) 25, 28, 213, 476, 556, 951, 966
FokI GGATG 2 cut(s) 47, 239
Fsp4HI GCNGC 7 cut(s) 25, 28, 213, 476, 556, 951, 966
FspBI CTAG 4 cut(s) 110, 479, 519, 891
GlaI GCGC 1 cut(s) 135
GluI GCNGC 7 cut(s) 25, 28, 213, 476, 556, 951, 966
GsuI CTGGAG 2 cut(s) 310, 628
HaeIII GGCC 2 cut(s) 490, 755
HapII CCGG 2 cut(s) 179, 344
HgaI GACGC 1 cut(s) 481
HhaI GCGC 1 cut(s) 136
Hin1II CATG 3 cut(s) 56, 341, 978
Hin6I GCGC 1 cut(s) 134
HinP1I GCGC 1 cut(s) 134
HindIII AAGCTT 2 cut(s) 69, 400
HinfI GANTC 5 cut(s) 426, 599, 667, 716, 854
HpaII CCGG 2 cut(s) 179, 344
HphI GGTGA 2 cut(s) 168, 608
Hpy188I TCNGA 2 cut(s) 312, 685
Hpy188III TCNNGA 4 cut(s) 53, 61, 97, 891
Hpy99I CGWCG 1 cut(s) 653
HpyAV CCTTC 4 cut(s) 276, 615, 814, 934
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 6 cut(s) 104, 256, 356, 846, 968, 978
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 735
HpyF3I CTNAG 4 cut(s) 446, 702, 909, 944
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 3 cut(s) 56, 341, 978
HspAI GCGC 1 cut(s) 134
Kzo9I GATC 5 cut(s) 6, 93, 537, 563, 744
LmnI GCTCC 2 cut(s) 38, 726
LpnPI CCDG 9 cut(s) 144, 192, 209, 274, 357, 555, 658, 746, 773
Lsp1109I GCAGC 7 cut(s) 36, 39, 199, 462, 542, 937, 952
MaeI CTAG 4 cut(s) 110, 479, 519, 891
MaeII ACGT 1 cut(s) 43
MaeIII GTNAC 2 cut(s) 124, 174
MalI GATC 5 cut(s) 8, 95, 539, 565, 746
MboI GATC 5 cut(s) 6, 93, 537, 563, 744
MboII GAAGA 9 cut(s) 70, 73, 103, 161, 485, 567, 704, 725, 836
MfeI CAATTG 1 cut(s) 430
MflI RGATCY 1 cut(s) 537
MhlI GDGCHC 1 cut(s) 43
MluCI AATT 9 cut(s) 137, 166, 201, 240, 430, 465, 588, 628, 635
MlyI GAGTC 1 cut(s) 863
MnlI CCTC 7 cut(s) 125, 285, 496, 501, 527, 646, 745
MroXI GAANNNNTTC 2 cut(s) 425, 671
MseI TTAA 5 cut(s) 11, 405, 798, 984, 1003
MslI CAYNNNNRTG 1 cut(s) 414
MspCI CTTAAG 1 cut(s) 10
MspI CCGG 2 cut(s) 179, 344
MspR9I CCNGG 1 cut(s) 761
MunI CAATTG 1 cut(s) 430
MvaI CCWGG 1 cut(s) 761
MwoI GCNNNNNNNGC 2 cut(s) 21, 735
NdeI CATATG 1 cut(s) 616
NdeII GATC 5 cut(s) 6, 93, 537, 563, 744
NlaIII CATG 3 cut(s) 56, 341, 978
NlaIV GGNNCC 2 cut(s) 539, 813
NmuCI GTSAC 1 cut(s) 174
PagI TCATGA 1 cut(s) 52
PdmI GAANNNNTTC 2 cut(s) 425, 671
PfeI GAWTC 4 cut(s) 426, 599, 667, 716
PfoI TCCNGGA 1 cut(s) 759
PkrI GCNGC 7 cut(s) 26, 29, 214, 477, 557, 952, 967
PleI GAGTC 1 cut(s) 862
PpsI GAGTC 1 cut(s) 862
PpuMI RGGWCCY 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 759
PspGI CCWGG 1 cut(s) 759
PspN4I GGNNCC 2 cut(s) 539, 813
PspPI GGNCC 3 cut(s) 189, 341, 488
PspPPI RGGWCCY 1 cut(s) 189
PsuI RGATCY 1 cut(s) 537
RseI CAYNNNNRTG 1 cut(s) 414
SaqAI TTAA 5 cut(s) 11, 405, 798, 984, 1003
SatI GCNGC 7 cut(s) 25, 28, 213, 476, 556, 951, 966
Sau3AI GATC 5 cut(s) 6, 93, 537, 563, 744
Sau96I GGNCC 3 cut(s) 189, 341, 488
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 1 cut(s) 761
SduI GDGCHC 1 cut(s) 43
SetI ASST 9 cut(s) 46, 73, 157, 194, 404, 520, 528, 557, 626
SinI GGWCC 2 cut(s) 189, 341
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
SpeI ACTAGT 1 cut(s) 109
Sse9I AATT 9 cut(s) 137, 166, 201, 240, 430, 465, 588, 628, 635
SsiI CCGC 1 cut(s) 724
SspMI CTAG 4 cut(s) 110, 479, 519, 891
StyD4I CCNGG 1 cut(s) 759
StyI CCWWGG 2 cut(s) 192, 541
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 46
TasI AATT 9 cut(s) 137, 166, 201, 240, 430, 465, 588, 628, 635
TfiI GAWTC 4 cut(s) 426, 599, 667, 716
Tru1I TTAA 5 cut(s) 11, 405, 798, 984, 1003
Tru9I TTAA 5 cut(s) 11, 405, 798, 984, 1003
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 7 cut(s) 24, 27, 212, 475, 555, 950, 965
Tsp45I GTSAC 1 cut(s) 174
TspDTI ATGAA 7 cut(s) 36, 41, 159, 747, 945, 945, 963
Vha464I CTTAAG 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 189, 341
XapI RAATTY 3 cut(s) 166, 465, 588
XbaI TCTAGA 1 cut(s) 890
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XmnI GAANNNNTTC 2 cut(s) 425, 671
XspI CTAG 4 cut(s) 110, 479, 519, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.