MD01G1180700.v1.1

DYAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001779
Physical Location & Seq
Forward (+)
27997009 .. 28000479
3471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1180700.v1.1.491

Sequence Viewer

Length: 2418 bp
ATGAGCAAGTACTTGGAGAAGAAGCAAGTCCAAAACAAGCCAGAAAGCAAAAGCACTTTTTTGGCCACACATGCTCTGCTCACCTCTGCTTCTGCTTCTCCTTCTTCCTCACCCGTTTCTTCTCTCGATGCTATCGAGCACATTAAAGTCGGCTGTTTCTACCAAATGGACCAGTCCAAGCTCCATGGAAGAACAGTACCAGAGCAGCTCAGGGCCGTACGGATCGTCATGGTGAGTGCGAAGGGCGCGTTTAAAGTGGCGGTGAGGTTTCCGAGCCTTCACTCTCTCCACGCTCATATCTCCGCAAAGGGGTATCCAAAACAAGACGCCAAGCAGATTCCGGCGCTCAACGAGAAGTATGTGATGTCCCCGGAAATGGCGTCGGAGGTGCTTTACCGGAGAATCCCACCACAGGAGATGGTAGATCGGAGCAACATTTGGAGCTTCTGGGCCGTTCAACCTCCGTTGAAGAATCACCCGAGAAGCACGTTGTCGAGCCCAGCAGTAAGTGGCGGAGGCGGAGCCATTACTAGCAGCGTGGTTCCTAAAAAGGGTCCGTGTTGGTCCGAGCTCACGTTTGCCGGAATGGTGAAGTGGGGCAAGCGCCGGCAGATTCGCTACCTCCGGAGGCACGAGTCGTCGCCGAGCTCTGGCTCTGACGATGAAGAAACGGAGAAGGGAAGGGAGGTTATGGAGCTGGAGGAAGATAGCGATGATGGTTTCACTGAGAAAGTAGTAACTGTGATAGACGAAGACGATGGCATTGAAGAAGAAGAAGAAGGTGAGGAGACAGAGGAAGAAGCTGCTCCTCATGTTCTGCCATGGAAGATGAACCTCCGGAGTCGGAAGCGGAAGCATCCCGATAATAAGAGAAAAATCAAATCTCCAACCCAAAAGCGATCAAAATCCAGAGCAAAGCCTCCGAATCAGAATAACCATATTGTAGCAGTTCCTGATCGCAGCAGCAGAAAATTGGCTAAAGTTAAACACACAGTAGCAAGATGGTCAGCAGGAAGGTACAAGCTGGCGGAGGAGAACATGTTGAAGGTGATGAAGGAGAAAGGGGCGGCGATTGGGAACCCAATACGGCGGCCGGCGCTGAGAGCGGAGGCTCGGAGGCTGATTGGAGACACGGGGTTACTGGATCATTTACTCAAACACATGGCGGGCAAGGTGGCGCCTGGTGGGACGGAGAGATTTAGGCGGCGGCACAACGCCGATGGAGCCATGGAGTATTGGCTGGAGAGTGCTGATCTGGTCCACATCCGGAAGGAGGCTGGGGTTCAAGATCCTTACTGGACCCCTCCGCCGGGGTGGAAGCCCGGCGATAGTCCCACCCAGGACCCCACTTGTGCAAGAGAGATTAAGGAGCTCCGTGAAGAAATTGACAAGATTAGAAGGGAGATGGAGGAGTCCAAGCAGCAAGGGAATGCTGGTTTGGCTATGGTGACCGCTTCAAATTCTAATTTGACCGGTGTTGACTTGGATCGTTACGGTTCATTAATCCCGATTAAGGAGGCTTGTGCAGAGCTGGAGAAGAAGATAGCTAAGATGGATGAAGAAAGGATCGAGTTTGTGACCAAAAAAGAAGAACAAATGATGGAGGTTACTCAATCTTTGTCCGGAATTAAGGAGAAAATCAGGATGTTCGAATCAAGCAAGGAAGATGCAGTCACAATTTCAGGAGCACCACCACCATCAGAAGCAAAACCAGCCGGTGAGAAGAAAGAAATAGATACACTAAGCGGCGGAGACAAGGCGGCTGCGGCGGCGGAGTGCAAGGCGGCAAAAATAGAGAGGCTGAGGAGTGGGTTCAGGATATGCAAGCCGCAAGGCACGTTTCTCTGGCCAGACATGGGCAGCCCCACCAACATTCCCAGTCCTTCTTCCCAGATTCTGGCCCCACCACATGACCTCTTTGTGGTCCCCACCCCACCCTCATCCTCCTCCTCCTCCTCCGCTCCCCGCCCCCTCATCAGCCCCACCACTCCCCCTGTGAAGCCGGTGGCTGAGCGGCGCCCAGTGACCACCACCACATTGTGCAATGTGACCACAATACCCTCTTCCACTCCTATTGTCGCTCCTCCACCTGTCCTGGCCCCACAGACCCAGAGTTCCACTACCGTCACCATCAGTAAAACCCTACTCATAAACCTCAACGAGCTCCCTGCAACAACAGCACCGAACCCTAACCCTAATCCCCAAACCAGCTGTGAAGCTATCACTACGCTCACCACCTACCACAGAAGACACCACCATCATCTGCCACGTATGGTGAAGGAGGAGGAGGAGGAGGACGACGAAACTGCAAGAAACGGTGATGATCAGCAGAGGCGGTGCGCCTCTCCTGCTTCCACCTGGAACGGAGAGAAATGGTGGATGGCTGTGGGAAATCCTAGCACTTCTTTGGAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0007049 GO:0008150 GO:0009987 GO:0022414 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0051321
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

806

Amino Acids

89.07

Weight (kDa)

8.87

Isoelectric Point (pI)

69.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 334 - 416 9.6e-26 Winged helix-turn-helix domain in plant reproductive proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1177, 2016
AccB7I CCANNNNNTGG 1 cut(s) 1897
AccBSI CCGCTC 3 cut(s) 1106, 1961, 2014
AccII CGCG 1 cut(s) 248
AccIII TCCGGA 4 cut(s) 624, 837, 1266, 1622
AclWI GGATC 5 cut(s) 230, 1152, 1283, 1494, 1574
AcoI YGGCCR 3 cut(s) 63, 1091, 1847
AcsI RAATTY 1 cut(s) 1459
AcyI GRCGYC 4 cut(s) 327, 380, 1178, 2017
AdeI CACNNNGTG 1 cut(s) 2040
AfaI GTAC 4 cut(s) 11, 198, 219, 1019
AflIII ACRYGT 1 cut(s) 1038
AgeI ACCGGT 1 cut(s) 1472
AgsI TTSAA 6 cut(s) 458, 469, 767, 1045, 1286, 1458
AjnI CCWGG 4 cut(s) 1180, 1338, 2094, 2357
AjuI GAANNNNNNNTTGG 2 cut(s) 1421, 1453
Alw21I GWGCWC 6 cut(s) 141, 573, 650, 1374, 1690, 2166
Alw26I GTCTC 3 cut(s) 782, 1122, 1746
AlwI GGATC 5 cut(s) 230, 1152, 1283, 1494, 1574
AlwNI CAGNNNCTG 2 cut(s) 953, 1897
Ama87I CYCGRG 1 cut(s) 478
Aor13HI TCCGGA 4 cut(s) 624, 837, 1266, 1622
AoxI GGCC 7 cut(s) 63, 213, 450, 1091, 1847, 1899, 2097
ApeKI GCWGC 8 cut(s) 205, 534, 803, 960, 963, 1420, 1763, 1860
ApoI RAATTY 1 cut(s) 1459
AseI ATTAAT 1 cut(s) 1502
AsiGI ACCGGT 1 cut(s) 1472
AspLEI GCGC 7 cut(s) 248, 346, 606, 1099, 1180, 2019, 2342
AsuC2I CCSGG 3 cut(s) 371, 1311, 1323
AsuII TTCGAA 1 cut(s) 1650
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
BalI TGGCCA 2 cut(s) 65, 1849
BanI GGYRCC 2 cut(s) 1177, 2016
BanII GRGCYC 5 cut(s) 500, 573, 650, 1374, 2166
BauI CACGAG 1 cut(s) 632
BbsI GAAGAC 2 cut(s) 759, 2254
Bbv12I GWGCWC 6 cut(s) 141, 573, 650, 1374, 1690, 2166
BbvCI CCTCAGC 1 cut(s) 1802
BbvI GCAGC 8 cut(s) 217, 546, 790, 972, 975, 1432, 1750, 1872
BceAI ACGGC 3 cut(s) 200, 437, 1103
BcgI CGANNNNNNTGC 4 cut(s) 2150, 2184, 2288, 2322
BciT130I CCWGG 4 cut(s) 1182, 1340, 2096, 2359
BciVI GTATCC 1 cut(s) 324
BclI TGATCA 1 cut(s) 2323
BcnI CCSGG 3 cut(s) 371, 1311, 1323
BcoDI GTCTC 3 cut(s) 782, 1122, 1746
BfaI CTAG 2 cut(s) 531, 2397
BfoI RGCGCY 5 cut(s) 347, 607, 1100, 1181, 2020
BfuI GTATCC 1 cut(s) 324
BlpI GCTNAGC 1 cut(s) 2010
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 7 cut(s) 371, 1182, 1311, 1323, 1340, 2096, 2359
Bme18I GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
BmeT110I CYCGRG 1 cut(s) 478
BmrFI CCNGG 7 cut(s) 371, 1182, 1311, 1323, 1340, 2096, 2359
BmrI ACTGGG 2 cut(s) 1872, 2015
BmsI GCATC 3 cut(s) 118, 865, 1657
BmuI ACTGGG 2 cut(s) 1872, 2015
BpiI GAAGAC 2 cut(s) 759, 2254
BpmI CTGGAG 3 cut(s) 719, 1262, 1553
Bpu10I CCTNAGC 2 cut(s) 209, 1802
Bpu1102I GCTNAGC 1 cut(s) 2010
Bpu14I TTCGAA 1 cut(s) 1650
BpuMI CCSGG 3 cut(s) 371, 1311, 1323
BsaAI YACGTR 1 cut(s) 2270
BsaBI GATNNNNATC 1 cut(s) 904
BsaHI GRCGYC 4 cut(s) 327, 380, 1178, 2017
BsaJI CCNNGG 6 cut(s) 184, 369, 821, 1227, 1310, 1338
BsaWI WCCGGW 6 cut(s) 396, 624, 837, 1266, 1472, 1622
BsaXI ACNNNNNCTCC 2 cut(s) 1025, 1055
Bse118I RCCGGY 5 cut(s) 606, 1093, 1472, 1715, 2002
Bse1I ACTGG 5 cut(s) 172, 1146, 1301, 1878, 2021
Bse3DI GCAATG 1 cut(s) 2050
Bse8I GATNNNNATC 1 cut(s) 904
BseAI TCCGGA 4 cut(s) 624, 837, 1266, 1622
BseBI CCWGG 4 cut(s) 1182, 1340, 2096, 2359
BseDI CCNNGG 6 cut(s) 184, 369, 821, 1227, 1310, 1338
BseGI GGATG 6 cut(s) 856, 1263, 1561, 1650, 1940, 2385
BseJI GATNNNNATC 1 cut(s) 904
BseMI GCAATG 1 cut(s) 2050
BseMII CTCAG 5 cut(s) 223, 717, 1091, 1793, 2001
BseNI ACTGG 5 cut(s) 172, 1146, 1301, 1878, 2021
BseX3I CGGCCG 1 cut(s) 1091
BseXI GCAGC 8 cut(s) 217, 546, 790, 972, 975, 1432, 1750, 1872
BseYI CCCAGC 2 cut(s) 499, 1277
BsgI GTGCAG 1 cut(s) 1545
Bsh1236I CGCG 1 cut(s) 248
Bsh1285I CGRYCG 1 cut(s) 1094
BshFI GGCC 7 cut(s) 65, 215, 452, 1093, 1849, 1901, 2099
BshNI GGYRCC 2 cut(s) 1177, 2016
BshTI ACCGGT 1 cut(s) 1472
BsiEI CGRYCG 1 cut(s) 1094
BsiHKAI GWGCWC 6 cut(s) 141, 573, 650, 1374, 1690, 2166
BsiHKCI CYCGRG 1 cut(s) 478
BsiWI CGTACG 1 cut(s) 217
BslFI GGGAC 4 cut(s) 352, 1201, 1317, 1910
BsmAI GTCTC 3 cut(s) 782, 1122, 1746
BsmFI GGGAC 4 cut(s) 352, 1201, 1317, 1910
BsmI GAATGC 1 cut(s) 1435
BsnI GGCC 7 cut(s) 65, 215, 452, 1093, 1849, 1901, 2099
BsoBI CYCGRG 1 cut(s) 478
Bsp119I TTCGAA 1 cut(s) 1650
Bsp1286I GDGCHC 7 cut(s) 141, 500, 573, 650, 1374, 1690, 2166
Bsp13I TCCGGA 4 cut(s) 624, 837, 1266, 1622
Bsp1720I GCTNAGC 1 cut(s) 2010
Bsp19I CCATGG 3 cut(s) 184, 821, 1227
BspANI GGCC 7 cut(s) 65, 215, 452, 1093, 1849, 1901, 2099
BspCNI CTCAG 5 cut(s) 222, 718, 1092, 1794, 2002
BspEI TCCGGA 4 cut(s) 624, 837, 1266, 1622
BspFNI CGCG 1 cut(s) 248
BspPI GGATC 5 cut(s) 230, 1152, 1283, 1494, 1574
BspT104I TTCGAA 1 cut(s) 1650
BspT107I GGYRCC 2 cut(s) 1177, 2016
BsrBI CCGCTC 3 cut(s) 1106, 1961, 2014
BsrDI GCAATG 1 cut(s) 2050
BsrFI RCCGGY 5 cut(s) 606, 1093, 1472, 1715, 2002
BsrI ACTGG 5 cut(s) 172, 1146, 1301, 1878, 2021
BssAI RCCGGY 5 cut(s) 606, 1093, 1472, 1715, 2002
BssECI CCNNGG 6 cut(s) 184, 369, 821, 1227, 1310, 1338
BssNI GRCGYC 4 cut(s) 327, 380, 1178, 2017
BssSI CACGAG 1 cut(s) 632
BssT1I CCWWGG 3 cut(s) 184, 821, 1227
Bst2BI CACGAG 1 cut(s) 632
Bst2UI CCWGG 4 cut(s) 1182, 1340, 2096, 2359
Bst4CI ACNGT 6 cut(s) 196, 742, 994, 1496, 2125, 2318
Bst6I CTCTTC 1 cut(s) 2068
BstACI GRCGYC 4 cut(s) 327, 380, 1178, 2017
BstBAI YACGTR 1 cut(s) 2270
BstBI TTCGAA 1 cut(s) 1650
BstC8I GCNNGC 6 cut(s) 602, 608, 1026, 1095, 1168, 1826
BstDEI CTNAG 7 cut(s) 209, 726, 1100, 1548, 1742, 1802, 2010
BstDSI CCRYGG 3 cut(s) 184, 821, 1227
BstEII GGTNACC 1 cut(s) 1447
BstF5I GGATG 6 cut(s) 856, 1263, 1561, 1650, 1940, 2385
BstFNI CGCG 1 cut(s) 248
BstH2I RGCGCY 5 cut(s) 347, 607, 1100, 1181, 2020
BstHHI GCGC 7 cut(s) 248, 346, 606, 1099, 1180, 2019, 2342
BstMAI GTCTC 3 cut(s) 782, 1122, 1746
BstMCI CGRYCG 1 cut(s) 1094
BstNI CCWGG 4 cut(s) 1182, 1340, 2096, 2359
BstNSI RCATGY 2 cut(s) 74, 1042
BstPI GGTNACC 1 cut(s) 1447
BstSCI CCNGG 7 cut(s) 369, 1180, 1309, 1321, 1338, 2094, 2357
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 8 cut(s) 217, 546, 790, 972, 975, 1432, 1750, 1872
BstV2I GAAGAC 2 cut(s) 759, 2254
BstX2I RGATCY 1 cut(s) 1288
BstYI RGATCY 1 cut(s) 1288
BstZI CGGCCG 1 cut(s) 1091
BsuI GTATCC 1 cut(s) 324
BsuRI GGCC 7 cut(s) 65, 215, 452, 1093, 1849, 1901, 2099
BtgI CCRYGG 3 cut(s) 184, 821, 1227
BtgZI GCGATG 1 cut(s) 726
BtsCI GGATG 6 cut(s) 856, 1263, 1561, 1650, 1940, 2385
BtsIMutI CAGTG 2 cut(s) 723, 2028
Cac8I GCNNGC 6 cut(s) 602, 608, 1026, 1095, 1168, 1826
CaiI CAGNNNCTG 2 cut(s) 953, 1897
CfoI GCGC 7 cut(s) 248, 346, 606, 1099, 1180, 2019, 2342
Cfr10I RCCGGY 5 cut(s) 606, 1093, 1472, 1715, 2002
CseI GACGC 2 cut(s) 335, 369
Csp6I GTAC 4 cut(s) 10, 197, 218, 1018
CspAI ACCGGT 1 cut(s) 1472
CspCI CAANNNNNGTGG 2 cut(s) 2056, 2091
CviQI GTAC 4 cut(s) 10, 197, 218, 1018
DdeI CTNAG 7 cut(s) 209, 726, 1100, 1548, 1742, 1802, 2010
DinI GGCGCC 2 cut(s) 1179, 2018
DraI TTTAAA 1 cut(s) 253
DraIII CACNNNGTG 1 cut(s) 2040
EaeI YGGCCR 3 cut(s) 63, 1091, 1847
EagI CGGCCG 1 cut(s) 1091
Eam1104I CTCTTC 1 cut(s) 2068
EarI CTCTTC 1 cut(s) 2068
EciI GGCGGA 6 cut(s) 528, 534, 1043, 1296, 1764, 1787
Ecl136II GAGCTC 4 cut(s) 571, 648, 1372, 2164
EclXI CGGCCG 1 cut(s) 1091
Eco130I CCWWGG 3 cut(s) 184, 821, 1227
Eco24I GRGCYC 5 cut(s) 500, 573, 650, 1374, 2166
Eco47I GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
Eco52I CGGCCG 1 cut(s) 1091
Eco53kI GAGCTC 4 cut(s) 571, 648, 1372, 2164
Eco88I CYCGRG 1 cut(s) 478
Eco91I GGTNACC 1 cut(s) 1447
EcoICRI GAGCTC 4 cut(s) 571, 648, 1372, 2164
EcoO109I RGGNCCY 1 cut(s) 1342
EcoO65I GGTNACC 1 cut(s) 1447
EcoRII CCWGG 4 cut(s) 1180, 1338, 2094, 2357
EcoT14I CCWWGG 3 cut(s) 184, 821, 1227
EcoT38I GRGCYC 5 cut(s) 500, 573, 650, 1374, 2166
EgeI GGCGCC 2 cut(s) 1179, 2018
EheI GGCGCC 2 cut(s) 1179, 2018
ErhI CCWWGG 3 cut(s) 184, 821, 1227
FaqI GGGAC 4 cut(s) 352, 1201, 1317, 1910
FauI CCCGC 2 cut(s) 1159, 1973
FbaI TGATCA 1 cut(s) 2323
FokI GGATG 6 cut(s) 843, 1250, 1568, 1657, 1927, 2392
FriOI GRGCYC 5 cut(s) 500, 573, 650, 1374, 2166
FspBI CTAG 2 cut(s) 531, 2397
GlaI GCGC 7 cut(s) 247, 345, 605, 1098, 1179, 2018, 2341
GsaI CCCAGC 2 cut(s) 503, 1281
GsuI CTGGAG 3 cut(s) 719, 1262, 1553
HaeII RGCGCY 5 cut(s) 347, 607, 1100, 1181, 2020
HaeIII GGCC 7 cut(s) 65, 215, 452, 1093, 1849, 1901, 2099
HgaI GACGC 2 cut(s) 335, 369
HhaI GCGC 7 cut(s) 248, 346, 606, 1099, 1180, 2019, 2342
Hin1I GRCGYC 4 cut(s) 327, 380, 1178, 2017
Hin6I GCGC 7 cut(s) 246, 344, 604, 1097, 1178, 2017, 2340
HinP1I GCGC 7 cut(s) 246, 344, 604, 1097, 1178, 2017, 2340
HincII GTYRAC 1 cut(s) 1480
HindII GTYRAC 1 cut(s) 1480
Hpy166II GTNNAC 2 cut(s) 1261, 1480
Hpy8I GTNNAC 2 cut(s) 1261, 1480
Hpy99I CGWCG 3 cut(s) 385, 643, 2303
HpyCH4III ACNGT 6 cut(s) 196, 742, 994, 1496, 2125, 2318
HpyCH4IV ACGT 4 cut(s) 488, 575, 1838, 2269
HpyCH4V TGCA 8 cut(s) 1355, 1526, 1670, 1779, 1824, 2043, 2171, 2309
HpyF3I CTNAG 7 cut(s) 209, 726, 1100, 1548, 1742, 1802, 2010
HpySE526I ACGT 4 cut(s) 488, 575, 1838, 2269
Hsp92I GRCGYC 4 cut(s) 327, 380, 1178, 2017
HspAI GCGC 7 cut(s) 246, 344, 604, 1097, 1178, 2017, 2340
KasI GGCGCC 2 cut(s) 1177, 2016
Kpn2I TCCGGA 4 cut(s) 624, 837, 1266, 1622
KroI GCCGGC 2 cut(s) 606, 1093
KroNI GCCGGC 2 cut(s) 608, 1095
Ksp22I TGATCA 1 cut(s) 2323
Lsp1109I GCAGC 8 cut(s) 217, 546, 790, 972, 975, 1432, 1750, 1872
LweI GCATC 3 cut(s) 118, 865, 1657
MaeI CTAG 2 cut(s) 531, 2397
MaeII ACGT 4 cut(s) 488, 575, 1838, 2269
MbiI CCGCTC 3 cut(s) 1106, 1961, 2014
MflI RGATCY 1 cut(s) 1288
MhlI GDGCHC 7 cut(s) 141, 500, 573, 650, 1374, 1690, 2166
MlsI TGGCCA 2 cut(s) 65, 1849
MluCI AATT 6 cut(s) 971, 1383, 1459, 1465, 1626, 1677
MluNI TGGCCA 2 cut(s) 65, 1849
Mly113I GGCGCC 2 cut(s) 1178, 2017
MlyI GAGTC 3 cut(s) 644, 850, 1421
MmeI TCCRAC 3 cut(s) 363, 824, 911
Mox20I TGGCCA 2 cut(s) 65, 1849
MroI TCCGGA 4 cut(s) 624, 837, 1266, 1622
MroNI GCCGGC 2 cut(s) 606, 1093
MscI TGGCCA 2 cut(s) 65, 1849
MseI TTAA 7 cut(s) 144, 252, 984, 1365, 1502, 1512, 1629
Msp20I TGGCCA 2 cut(s) 65, 1849
MspA1I CMGCKG 1 cut(s) 2211
MspR9I CCNGG 7 cut(s) 371, 1182, 1311, 1323, 1340, 2096, 2359
Mva1269I GAATGC 1 cut(s) 1435
MvaI CCWGG 4 cut(s) 1182, 1340, 2096, 2359
MvnI CGCG 1 cut(s) 248
NaeI GCCGGC 2 cut(s) 608, 1095
NarI GGCGCC 2 cut(s) 1178, 2017
NciI CCSGG 3 cut(s) 371, 1311, 1323
NcoI CCATGG 3 cut(s) 184, 821, 1227
NgoMIV GCCGGC 2 cut(s) 606, 1093
NmeAIII GCCGAG 1 cut(s) 669
NmuCI GTSAC 6 cut(s) 1447, 1576, 1672, 2023, 2047, 2125
NspI RCATGY 2 cut(s) 74, 1042
NspV TTCGAA 1 cut(s) 1650
PciI ACATGT 1 cut(s) 1038
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 1 cut(s) 1435
PdiI GCCGGC 2 cut(s) 608, 1095
PfeI GAWTC 7 cut(s) 337, 402, 472, 613, 925, 1652, 1894
Pfl23II CGTACG 1 cut(s) 217
PflMI CCANNNNNTGG 1 cut(s) 1897
PinAI ACCGGT 1 cut(s) 1472
PleI GAGTC 3 cut(s) 643, 849, 1420
PluTI GGCGCC 2 cut(s) 1181, 2020
PpsI GAGTC 3 cut(s) 643, 849, 1420
Ppu21I YACGTR 1 cut(s) 2270
PpuMI RGGWCCY 1 cut(s) 1342
PscI ACATGT 1 cut(s) 1038
PshBI ATTAAT 1 cut(s) 1502
Psp124BI GAGCTC 4 cut(s) 573, 650, 1374, 2166
Psp5II RGGWCCY 1 cut(s) 1342
Psp6I CCWGG 4 cut(s) 1180, 1338, 2094, 2357
PspEI GGTNACC 1 cut(s) 1447
PspFI CCCAGC 2 cut(s) 499, 1277
PspGI CCWGG 4 cut(s) 1180, 1338, 2094, 2357
PspLI CGTACG 1 cut(s) 217
PspPPI RGGWCCY 1 cut(s) 1342
PstNI CAGNNNCTG 2 cut(s) 953, 1897
PsuI RGATCY 1 cut(s) 1288
PvuII CAGCTG 1 cut(s) 2211
RsaI GTAC 4 cut(s) 11, 198, 219, 1019
RsaNI GTAC 4 cut(s) 10, 197, 218, 1018
SacI GAGCTC 4 cut(s) 573, 650, 1374, 2166
SaqAI TTAA 7 cut(s) 144, 252, 984, 1365, 1502, 1512, 1629
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 3 cut(s) 644, 850, 1421
ScrFI CCNGG 7 cut(s) 371, 1182, 1311, 1323, 1340, 2096, 2359
SduI GDGCHC 7 cut(s) 141, 500, 573, 650, 1374, 1690, 2166
SfaNI GCATC 3 cut(s) 118, 865, 1657
SfoI GGCGCC 2 cut(s) 1179, 2018
SfuI TTCGAA 1 cut(s) 1650
SinI GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
Sse9I AATT 6 cut(s) 971, 1383, 1459, 1465, 1626, 1677
SspDI GGCGCC 2 cut(s) 1177, 2016
SspMI CTAG 2 cut(s) 531, 2397
SstI GAGCTC 4 cut(s) 573, 650, 1374, 2166
StyD4I CCNGG 7 cut(s) 369, 1180, 1309, 1321, 1338, 2094, 2357
StyI CCWWGG 3 cut(s) 184, 821, 1227
TaaI ACNGT 6 cut(s) 196, 742, 994, 1496, 2125, 2318
TaiI ACGT 4 cut(s) 491, 578, 1841, 2272
TaqI TCGA 5 cut(s) 126, 135, 494, 1569, 1650
TasI AATT 6 cut(s) 971, 1383, 1459, 1465, 1626, 1677
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 7 cut(s) 337, 402, 472, 613, 925, 1652, 1894
Tru1I TTAA 7 cut(s) 144, 252, 984, 1365, 1502, 1512, 1629
Tru9I TTAA 7 cut(s) 144, 252, 984, 1365, 1502, 1512, 1629
TscAI CASTG 2 cut(s) 730, 2028
TseFI GTSAC 6 cut(s) 1447, 1576, 1672, 2023, 2047, 2125
TseI GCWGC 8 cut(s) 205, 534, 803, 960, 963, 1420, 1763, 1860
Tsp45I GTSAC 6 cut(s) 1447, 1576, 1672, 2023, 2047, 2125
TspDTI ATGAA 5 cut(s) 678, 845, 1067, 1488, 1572
TspGWI ACGGA 7 cut(s) 235, 453, 546, 686, 1205, 1364, 2379
TspRI CASTG 2 cut(s) 730, 2028
Van91I CCANNNNNTGG 1 cut(s) 1897
VpaK11BI GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
VspI ATTAAT 1 cut(s) 1502
XapI RAATTY 1 cut(s) 1459
XceI RCATGY 2 cut(s) 74, 1042
XspI CTAG 2 cut(s) 531, 2397
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.