Rh1BG354600

DYAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001779
Physical Location & Seq
Forward (+)
48624888 .. 48628431
3544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG354600.1

Sequence Viewer

Length: 1248 bp
ATGAAGAAGTACTTGGAGAACAAGCATCAAGAACAAAGAGCACAAAGCCTTTGTTTGGCAACACATGCTTCTGCTTCTCAAGAGACCATCGACCACATCCATGTGGGCTCGTTCTATGAACTAGACCATTCGAAGCTTCCTCCTATTGCCTCAGAAGAGATGAAGTCGGTTCGGGTCGTCATGGTGAATGGAAAGTCTCTGTTCAAGGTTTCAGTGAGGTTTCCAAGCCTTCACTCTCTCCAAACCCATCTGAATGAGGAAAGCTACCAAAACCCAGACGGCTTCCTTCCTGCTTTCGACGAAAACTACTCTATGGCTCCACAAATCGCGTCGGAGGTGCTTTACCGGAGAATCCCACATCACGAAATAGCAGAGCAGAGCATGATGTGGAGTTTTTGGGCCGGACGGCGTCTTTTCGCTACCCGTAAAAGCCCATCTCCGTGTTGCTTGTCGGAGATTAAGCTCGGCGGGATGGTCCAATGGGGTAAGCGCAAACTAGTCCGGTGCGTTACGAGGCATGAGAGTCTGTCGAGCTCTGACCAAGAACAACCAGAGAAGGAAGAAACGGAGATGGATGAAGATGTGAAAGAGCCTCGCTATGAGTTTAGGAAGAGAAAACTGGTTCAAAAGAAGACCAAAGCAGCGAAGCGTCGGAAGCAGAACAATCTAATAGTGGTTCATGACCGGACCCAAAATCAATTGGTTGAAGCCTCAATAGAGAGATGGACTGCAAGGAGGTATAAGGCAGCAGAGGAGAGCATACTTAAGGTGATGAAGGCAAAAGGTCTTTGTGCTGAGAATCCAATACGGAGAGGACCACTGAGGTGTGAGGCCCGTAAGTTGATTGGGGACACTGGTTTGATAGACCATTTGTTGAAGCATATGGCTGGGAAGGTAGCTCCGGGTGGCACTGATCGGTTTCGGAGAAGGCATAATGCTGATGGAGCACTTGTGTATTGGCTGGAGAGTGCTGATTTAATGAGTGTAAGGAAGGAGGCTGGGGTTGAGGATCCTTATTGGACTCCACCCCCGGGGTGGAAGAATGGGGATAACCCAACTCAGGACCCTACTTGTGTGAGGATGTTCAAGGAGATTTGGGAAGAGATAGCCAATGTGAAAAGAGAGAGGGAATTTCAGCAGCAAGAGGATATAGCTATGGTGACCACCTTAAACTCTTCGCTGACAACTCTGGATCAGGGCCAAGCTTACAGTTCAGTAACCTATCAAAAGGTAAATGATGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0007049 GO:0008150 GO:0009987 GO:0022414 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0051321
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

415

Amino Acids

47.84

Weight (kDa)

8.96

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 241 - 323 4.9e-24 Winged helix-turn-helix domain in plant reproductive proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 329
AciI CCGC 1 cut(s) 468
AclWI GGATC 3 cut(s) 1004, 1017, 1200
AcsI RAATTY 1 cut(s) 1130
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 4 cut(s) 55, 1031, 1035, 1060
AflII CTTAAG 1 cut(s) 764
AgsI TTSAA 5 cut(s) 205, 626, 707, 877, 1087
AhlI ACTAGT 1 cut(s) 496
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AleI CACNNNNGTG 1 cut(s) 823
AluBI AGCT 7 cut(s) 136, 264, 463, 534, 899, 1154, 1205
AluI AGCT 7 cut(s) 136, 264, 463, 534, 899, 1154, 1205
Alw21I GWGCWC 3 cut(s) 43, 536, 949
Alw26I GTCTC 2 cut(s) 77, 201
AlwI GGATC 3 cut(s) 1004, 1017, 1200
Ama87I CYCGRG 1 cut(s) 1030
AoxI GGCC 3 cut(s) 399, 831, 1198
ApeKI GCWGC 3 cut(s) 641, 746, 1138
ApoI RAATTY 1 cut(s) 1130
AspLEI GCGC 1 cut(s) 492
AspS9I GGNCC 7 cut(s) 399, 475, 687, 815, 832, 1063, 1198
AsuC2I CCSGG 3 cut(s) 903, 1031, 1032
AsuHPI GGTGA 3 cut(s) 196, 781, 1171
AsuII TTCGAA 1 cut(s) 131
AvaI CYCGRG 1 cut(s) 1030
AvaII GGWCC 4 cut(s) 475, 687, 815, 1063
BamHI GGATCC 1 cut(s) 1009
BanII GRGCYC 2 cut(s) 110, 536
BbsI GAAGAC 1 cut(s) 638
Bbv12I GWGCWC 3 cut(s) 43, 536, 949
BbvI GCAGC 3 cut(s) 653, 758, 1150
BccI CCATC 7 cut(s) 95, 255, 442, 466, 565, 717, 935
BceAI ACGGC 2 cut(s) 295, 422
BcnI CCSGG 3 cut(s) 903, 1031, 1032
BcoDI GTCTC 2 cut(s) 77, 201
BcuI ACTAGT 1 cut(s) 496
BfaI CTAG 2 cut(s) 122, 497
BfrI CTTAAG 1 cut(s) 764
BisI GCNGC 3 cut(s) 642, 747, 1139
BlsI GCNGC 3 cut(s) 643, 748, 1140
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 3 cut(s) 903, 1031, 1032
Bme18I GGWCC 4 cut(s) 475, 687, 815, 1063
BmeT110I CYCGRG 1 cut(s) 1030
BmgT120I GGNCC 7 cut(s) 399, 475, 687, 815, 832, 1063, 1198
BmiI GGNNCC 4 cut(s) 318, 689, 1011, 1065
BmrFI CCNGG 3 cut(s) 903, 1031, 1032
BmsI GCATC 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 638
BpmI CTGGAG 1 cut(s) 983
Bpu14I TTCGAA 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 63
BpuMI CCSGG 3 cut(s) 903, 1031, 1032
BsaHI GRCGYC 1 cut(s) 409
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 3 cut(s) 1029, 1030, 1031
BsaWI WCCGGW 3 cut(s) 345, 501, 684
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 1031, 1035, 1060
Bse1I ACTGG 2 cut(s) 624, 859
BseDI CCNNGG 3 cut(s) 1029, 1030, 1031
BseGI GGATG 4 cut(s) 96, 477, 580, 1086
BseLI CCNNNNNNNGG 4 cut(s) 55, 1031, 1035, 1060
BseMII CTCAG 4 cut(s) 165, 786, 812, 1073
BseNI ACTGG 2 cut(s) 624, 859
BseRI GAGGAG 1 cut(s) 767
BseXI GCAGC 3 cut(s) 653, 758, 1150
BseYI CCCAGC 2 cut(s) 887, 998
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 3 cut(s) 401, 833, 1200
BsiHKAI GWGCWC 3 cut(s) 43, 536, 949
BsiHKCI CYCGRG 1 cut(s) 1030
BsiSI CCGG 6 cut(s) 346, 402, 502, 685, 902, 1031
BslFI GGGAC 1 cut(s) 863
BslI CCNNNNNNNGG 4 cut(s) 55, 1031, 1035, 1060
BsmAI GTCTC 2 cut(s) 77, 201
BsmFI GGGAC 1 cut(s) 863
BsnI GGCC 3 cut(s) 401, 833, 1200
Bso31I GGTCTC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 1030
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 4 cut(s) 43, 110, 536, 949
Bsp143I GATC 3 cut(s) 913, 1009, 1192
BspACI CCGC 1 cut(s) 468
BspANI GGCC 3 cut(s) 401, 833, 1200
BspCNI CTCAG 4 cut(s) 164, 787, 813, 1072
BspFNI CGCG 1 cut(s) 329
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 4 cut(s) 318, 689, 1011, 1065
BspPI GGATC 3 cut(s) 1004, 1017, 1200
BspT104I TTCGAA 1 cut(s) 131
BspTI CTTAAG 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 77
BsrI ACTGG 2 cut(s) 624, 859
BssECI CCNNGG 3 cut(s) 1029, 1030, 1031
BssMI GATC 3 cut(s) 913, 1009, 1192
BssNI GRCGYC 1 cut(s) 409
Bst4CI ACNGT 1 cut(s) 1211
Bst6I CTCTTC 4 cut(s) 150, 605, 1095, 1180
BstACI GRCGYC 1 cut(s) 409
BstAFI CTTAAG 1 cut(s) 764
BstAPI GCANNNNNTGC 1 cut(s) 65
BstBI TTCGAA 1 cut(s) 131
BstDEI CTNAG 4 cut(s) 151, 795, 821, 1059
BstEII GGTNACC 1 cut(s) 1159
BstF5I GGATG 4 cut(s) 96, 477, 580, 1086
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 1 cut(s) 492
BstKTI GATC 3 cut(s) 916, 1012, 1195
BstMAI GTCTC 2 cut(s) 77, 201
BstMBI GATC 3 cut(s) 913, 1009, 1192
BstMWI GCNNNNNNNGC 3 cut(s) 65, 655, 944
BstNSI RCATGY 1 cut(s) 68
BstPI GGTNACC 1 cut(s) 1159
BstSCI CCNGG 3 cut(s) 901, 1029, 1030
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 3 cut(s) 653, 758, 1150
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BsuRI GGCC 3 cut(s) 401, 833, 1200
BtsCI GGATG 4 cut(s) 96, 477, 580, 1086
BtsIMutI CAGTG 4 cut(s) 219, 818, 852, 909
CciI TCATGA 1 cut(s) 679
CfoI GCGC 1 cut(s) 492
Cfr13I GGNCC 7 cut(s) 399, 475, 687, 815, 832, 1063, 1198
Cfr9I CCCGGG 1 cut(s) 1030
CseI GACGC 3 cut(s) 318, 398, 638
Csp6I GTAC 1 cut(s) 10
CviAII CATG 6 cut(s) 65, 101, 181, 382, 518, 680
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 4 cut(s) 151, 795, 821, 1059
DpnI GATC 3 cut(s) 915, 1011, 1194
DpnII GATC 3 cut(s) 913, 1009, 1192
Eam1104I CTCTTC 4 cut(s) 150, 605, 1095, 1180
EarI CTCTTC 4 cut(s) 150, 605, 1095, 1180
Ecl136II GAGCTC 1 cut(s) 534
Eco24I GRGCYC 2 cut(s) 110, 536
Eco31I GGTCTC 1 cut(s) 77
Eco47I GGWCC 4 cut(s) 475, 687, 815, 1063
Eco53kI GAGCTC 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 1030
Eco91I GGTNACC 1 cut(s) 1159
EcoICRI GAGCTC 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 1063
EcoO65I GGTNACC 1 cut(s) 1159
EcoT38I GRGCYC 2 cut(s) 110, 536
FaeI CATG 6 cut(s) 68, 104, 184, 385, 521, 683
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 1 cut(s) 863
FatI CATG 6 cut(s) 64, 100, 180, 381, 517, 679
FauI CCCGC 1 cut(s) 461
FauNDI CATATG 1 cut(s) 882
Fnu4HI GCNGC 3 cut(s) 642, 747, 1139
FokI GGATG 4 cut(s) 83, 484, 587, 1093
FriOI GRGCYC 2 cut(s) 110, 536
Fsp4HI GCNGC 3 cut(s) 642, 747, 1139
FspBI CTAG 2 cut(s) 122, 497
GlaI GCGC 1 cut(s) 491
GluI GCNGC 3 cut(s) 642, 747, 1139
GsaI CCCAGC 2 cut(s) 891, 1002
GsuI CTGGAG 1 cut(s) 983
HaeIII GGCC 3 cut(s) 401, 833, 1200
HapII CCGG 6 cut(s) 346, 402, 502, 685, 902, 1031
HgaI GACGC 3 cut(s) 318, 398, 638
HhaI GCGC 1 cut(s) 492
Hin1I GRCGYC 1 cut(s) 409
Hin1II CATG 6 cut(s) 68, 104, 184, 385, 521, 683
Hin6I GCGC 1 cut(s) 490
HinP1I GCGC 1 cut(s) 490
HindIII AAGCTT 2 cut(s) 134, 1203
HinfI GANTC 4 cut(s) 351, 523, 799, 1021
HpaII CCGG 6 cut(s) 346, 402, 502, 685, 902, 1031
HphI GGTGA 3 cut(s) 196, 781, 1171
Hpy188I TCNGA 7 cut(s) 154, 252, 334, 454, 538, 654, 924
Hpy188III TCNNGA 6 cut(s) 29, 80, 362, 680, 1061, 1190
Hpy99I CGWCG 3 cut(s) 302, 334, 654
HpyAV CCTTC 7 cut(s) 239, 296, 550, 769, 886, 921, 985
HpyCH4III ACNGT 1 cut(s) 1211
HpyCH4V TGCA 1 cut(s) 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 655, 944
HpyF3I CTNAG 4 cut(s) 151, 795, 821, 1059
Hsp92I GRCGYC 1 cut(s) 409
Hsp92II CATG 6 cut(s) 68, 104, 184, 385, 521, 683
HspAI GCGC 1 cut(s) 490
Kzo9I GATC 3 cut(s) 913, 1009, 1192
LmnI GCTCC 3 cut(s) 322, 904, 944
Lsp1109I GCAGC 3 cut(s) 653, 758, 1150
LweI GCATC 1 cut(s) 34
MaeI CTAG 2 cut(s) 122, 497
MaeIII GTNAC 3 cut(s) 508, 1159, 1216
MalI GATC 3 cut(s) 915, 1011, 1194
MboI GATC 3 cut(s) 913, 1009, 1192
MboII GAAGA 9 cut(s) 16, 167, 572, 590, 622, 643, 1051, 1112, 1167
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 4 cut(s) 43, 110, 536, 949
MluCI AATT 2 cut(s) 698, 1130
MlyI GAGTC 2 cut(s) 532, 1015
MmeI TCCRAC 3 cut(s) 312, 432, 632
MseI TTAA 4 cut(s) 459, 765, 977, 1169
MslI CAYNNNNRTG 5 cut(s) 99, 101, 252, 439, 823
MspCI CTTAAG 1 cut(s) 764
MspI CCGG 6 cut(s) 346, 402, 502, 685, 902, 1031
MspR9I CCNGG 3 cut(s) 903, 1031, 1032
MunI CAATTG 1 cut(s) 698
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 3 cut(s) 65, 655, 944
NciI CCSGG 3 cut(s) 903, 1031, 1032
NdeI CATATG 1 cut(s) 882
NdeII GATC 3 cut(s) 913, 1009, 1192
NlaIII CATG 6 cut(s) 68, 104, 184, 385, 521, 683
NlaIV GGNNCC 4 cut(s) 318, 689, 1011, 1065
NmeAIII GCCGAG 1 cut(s) 444
NmuCI GTSAC 1 cut(s) 1159
NspI RCATGY 1 cut(s) 68
NspV TTCGAA 1 cut(s) 131
OliI CACNNNNGTG 1 cut(s) 823
PagI TCATGA 1 cut(s) 679
PfeI GAWTC 2 cut(s) 351, 799
PflFI GACNNNGTC 1 cut(s) 408
PkrI GCNGC 3 cut(s) 643, 748, 1140
PleI GAGTC 2 cut(s) 531, 1015
PpsI GAGTC 2 cut(s) 531, 1015
PpuMI RGGWCCY 1 cut(s) 1063
Psp124BI GAGCTC 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 1063
PspEI GGTNACC 1 cut(s) 1159
PspFI CCCAGC 2 cut(s) 887, 998
PspN4I GGNNCC 4 cut(s) 318, 689, 1011, 1065
PspPI GGNCC 7 cut(s) 399, 475, 687, 815, 832, 1063, 1198
PspPPI RGGWCCY 1 cut(s) 1063
PsuI RGATCY 1 cut(s) 1009
PsyI GACNNNGTC 1 cut(s) 408
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 5 cut(s) 99, 101, 252, 439, 823
SacI GAGCTC 1 cut(s) 536
SaqAI TTAA 4 cut(s) 459, 765, 977, 1169
SatI GCNGC 3 cut(s) 642, 747, 1139
Sau3AI GATC 3 cut(s) 913, 1009, 1192
Sau96I GGNCC 7 cut(s) 399, 475, 687, 815, 832, 1063, 1198
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 2 cut(s) 532, 1015
ScrFI CCNGG 3 cut(s) 903, 1031, 1032
SduI GDGCHC 4 cut(s) 43, 110, 536, 949
SfaNI GCATC 1 cut(s) 34
SfuI TTCGAA 1 cut(s) 131
SinI GGWCC 4 cut(s) 475, 687, 815, 1063
SmaI CCCGGG 1 cut(s) 1032
SmiMI CAYNNNNRTG 5 cut(s) 99, 101, 252, 439, 823
SmlI CTYRAG 2 cut(s) 78, 764
SmoI CTYRAG 2 cut(s) 78, 764
SpeI ACTAGT 1 cut(s) 496
Sse9I AATT 2 cut(s) 698, 1130
SsiI CCGC 1 cut(s) 468
SspMI CTAG 2 cut(s) 122, 497
SstI GAGCTC 1 cut(s) 536
StyD4I CCNGG 3 cut(s) 901, 1029, 1030
TaaI ACNGT 1 cut(s) 1211
TaqI TCGA 4 cut(s) 90, 131, 297, 530
TasI AATT 2 cut(s) 698, 1130
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 2 cut(s) 351, 799
Tru1I TTAA 4 cut(s) 459, 765, 977, 1169
Tru9I TTAA 4 cut(s) 459, 765, 977, 1169
TscAI CASTG 4 cut(s) 219, 825, 859, 916
TseFI GTSAC 1 cut(s) 1159
TseI GCWGC 3 cut(s) 641, 746, 1138
Tsp45I GTSAC 1 cut(s) 1159
TspDTI ATGAA 6 cut(s) 17, 132, 176, 591, 668, 788
TspGWI ACGGA 3 cut(s) 429, 581, 823
TspMI CCCGGG 1 cut(s) 1030
TspRI CASTG 4 cut(s) 219, 825, 859, 916
Tth111I GACNNNGTC 1 cut(s) 408
Vha464I CTTAAG 1 cut(s) 764
VpaK11BI GGWCC 4 cut(s) 475, 687, 815, 1063
XapI RAATTY 1 cut(s) 1130
XceI RCATGY 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 1 cut(s) 1032
XmaI CCCGGG 1 cut(s) 1030
XspI CTAG 2 cut(s) 122, 497
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.