Rmu_sc0008322.1_g000013

DYAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008322.1
OG0001779
Physical Location & Seq
Forward (+)
65530 .. 65937
408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008322.1_g000013.1.cds

Sequence Viewer

Length: 408 bp
atgaaggcgaaaagtgcttgtgccgagaatccaattcagagagcaccactgaggtctgaggcccggaagttgataggggacactggtttggtggaccatttgttaaagcatatggctgggaaggtagctccgggtggcgctgatcggtttcggagaaggcataatgctgatggagcacttatgtattggctggagagtgctgagttgatgagtctaaggaaggaggctggggttgaggatccttattggactccaccccccgggtggaaggttggggataaccaaactcaggaccctacttgtgtgaggatgttcaaggagatttgggaagagatagccaatgtgaaaaggtacggtaatgggatcaattaccttgttattgttgaatctgtttggattgtagagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0007049 GO:0008150 GO:0009987 GO:0022414 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0051321
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

15.26

Weight (kDa)

7.79

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 233, 246, 371
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 3 cut(s) 260, 264, 289
AgsI TTSAA 2 cut(s) 316, 386
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw21I GWGCWC 2 cut(s) 46, 178
AlwI GGATC 3 cut(s) 233, 246, 371
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 1 cut(s) 60
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 3 cut(s) 61, 94, 292
AsuC2I CCSGG 4 cut(s) 64, 132, 261, 262
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 2 cut(s) 94, 292
BamHI GGATCC 1 cut(s) 238
Bbv12I GWGCWC 2 cut(s) 46, 178
BccI CCATC 1 cut(s) 164
BcnI CCSGG 4 cut(s) 64, 132, 261, 262
BfoI RGCGCY 1 cut(s) 141
Bme1390I CCNGG 4 cut(s) 64, 132, 261, 262
Bme18I GGWCC 2 cut(s) 94, 292
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 3 cut(s) 61, 94, 292
BmiI GGNNCC 2 cut(s) 240, 294
BmrFI CCNGG 4 cut(s) 64, 132, 261, 262
BpmI CTGGAG 1 cut(s) 212
BpuMI CCSGG 4 cut(s) 64, 132, 261, 262
BsaJI CCNNGG 2 cut(s) 259, 260
Bsc4I CCNNNNNNNGG 3 cut(s) 260, 264, 289
Bse1I ACTGG 1 cut(s) 88
BseDI CCNNGG 2 cut(s) 259, 260
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 3 cut(s) 260, 264, 289
BseMII CTCAG 4 cut(s) 41, 48, 192, 302
BseNI ACTGG 1 cut(s) 88
BseYI CCCAGC 2 cut(s) 116, 227
BshFI GGCC 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 46, 178
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 3 cut(s) 64, 131, 261
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 3 cut(s) 260, 264, 289
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 2 cut(s) 46, 178
Bsp143I GATC 3 cut(s) 142, 238, 363
BspANI GGCC 1 cut(s) 62
BspCNI CTCAG 4 cut(s) 42, 49, 193, 301
BspLI GGNNCC 2 cut(s) 240, 294
BspPI GGATC 3 cut(s) 233, 246, 371
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 2 cut(s) 259, 260
BssMI GATC 3 cut(s) 142, 238, 363
Bst4CI ACNGT 1 cut(s) 356
Bst6I CTCTTC 1 cut(s) 324
BstDEI CTNAG 5 cut(s) 50, 57, 201, 215, 288
BstF5I GGATG 1 cut(s) 315
BstH2I RGCGCY 1 cut(s) 141
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 3 cut(s) 145, 241, 366
BstMBI GATC 3 cut(s) 142, 238, 363
BstMWI GCNNNNNNNGC 2 cut(s) 14, 173
BstSCI CCNGG 4 cut(s) 62, 130, 259, 260
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 1 cut(s) 62
BtsCI GGATG 1 cut(s) 315
BtsIMutI CAGTG 2 cut(s) 47, 81
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 3 cut(s) 61, 94, 292
Cfr9I CCCGGG 1 cut(s) 260
Csp6I GTAC 1 cut(s) 352
CviJI RGCY 6 cut(s) 62, 116, 128, 190, 227, 338
CviKI_1 RGCY 6 cut(s) 62, 116, 128, 190, 227, 338
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 5 cut(s) 50, 57, 201, 215, 288
DpnI GATC 3 cut(s) 144, 240, 365
DpnII GATC 3 cut(s) 142, 238, 363
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco47I GGWCC 2 cut(s) 94, 292
Eco88I CYCGRG 1 cut(s) 260
EcoO109I RGGNCCY 1 cut(s) 292
FaiI YATR 4 cut(s) 111, 113, 162, 182
FaqI GGGAC 1 cut(s) 92
FauNDI CATATG 1 cut(s) 111
FokI GGATG 1 cut(s) 322
GlaI GCGC 1 cut(s) 139
GsaI CCCAGC 2 cut(s) 120, 231
GsuI CTGGAG 1 cut(s) 212
HaeII RGCGCY 1 cut(s) 141
HaeIII GGCC 1 cut(s) 62
HapII CCGG 3 cut(s) 64, 131, 261
HhaI GCGC 1 cut(s) 140
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HinfI GANTC 4 cut(s) 28, 211, 250, 386
HpaII CCGG 3 cut(s) 64, 131, 261
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 3 cut(s) 39, 58, 153
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 4 cut(s) 115, 150, 214, 262
HpyCH4III ACNGT 1 cut(s) 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 173
HpyF3I CTNAG 5 cut(s) 50, 57, 201, 215, 288
HspAI GCGC 1 cut(s) 138
Kzo9I GATC 3 cut(s) 142, 238, 363
LmnI GCTCC 2 cut(s) 133, 173
LpnPI CCDG 8 cut(s) 69, 77, 102, 144, 176, 213, 274, 275
MalI GATC 3 cut(s) 144, 240, 365
MboI GATC 3 cut(s) 142, 238, 363
MboII GAAGA 1 cut(s) 341
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 2 cut(s) 46, 178
MluCI AATT 2 cut(s) 33, 367
MlyI GAGTC 2 cut(s) 220, 244
MnlI CCTC 5 cut(s) 45, 52, 217, 229, 300
MseI TTAA 1 cut(s) 104
MspI CCGG 3 cut(s) 64, 131, 261
MspR9I CCNGG 4 cut(s) 64, 132, 261, 262
MwoI GCNNNNNNNGC 2 cut(s) 14, 173
NciI CCSGG 4 cut(s) 64, 132, 261, 262
NdeI CATATG 1 cut(s) 111
NdeII GATC 3 cut(s) 142, 238, 363
NlaIV GGNNCC 2 cut(s) 240, 294
NmeAIII GCCGAG 1 cut(s) 49
PfeI GAWTC 2 cut(s) 28, 386
PleI GAGTC 2 cut(s) 219, 244
PpsI GAGTC 2 cut(s) 219, 244
PpuMI RGGWCCY 1 cut(s) 292
Psp5II RGGWCCY 1 cut(s) 292
PspFI CCCAGC 2 cut(s) 116, 227
PspN4I GGNNCC 2 cut(s) 240, 294
PspPI GGNCC 3 cut(s) 61, 94, 292
PspPPI RGGWCCY 1 cut(s) 292
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
SaqAI TTAA 1 cut(s) 104
Sau3AI GATC 3 cut(s) 142, 238, 363
Sau96I GGNCC 3 cut(s) 61, 94, 292
SchI GAGTC 2 cut(s) 220, 244
ScrFI CCNGG 4 cut(s) 64, 132, 261, 262
SduI GDGCHC 2 cut(s) 46, 178
SetI ASST 6 cut(s) 56, 126, 130, 273, 353, 375
SinI GGWCC 2 cut(s) 94, 292
SmaI CCCGGG 1 cut(s) 262
Sse9I AATT 2 cut(s) 33, 367
StyD4I CCNGG 4 cut(s) 62, 130, 259, 260
TaaI ACNGT 1 cut(s) 356
TasI AATT 2 cut(s) 33, 367
TfiI GAWTC 2 cut(s) 28, 386
Tru1I TTAA 1 cut(s) 104
Tru9I TTAA 1 cut(s) 104
TscAI CASTG 2 cut(s) 54, 88
TspDTI ATGAA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 260
TspRI CASTG 2 cut(s) 54, 88
VpaK11BI GGWCC 2 cut(s) 94, 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 261
XmaI CCCGGG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.