pycom01g19090

DYAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001779
Physical Location & Seq
Forward (+)
17894776 .. 17897934
3159 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g19090.1

Sequence Viewer

Length: 2412 bp
ATGAGCAAGTACTTGGAGAAGAAGCAAGTCCAAAAAAAGCCAGAATGCAAAAGCACTTTTTTGGCCACATATGCGCTGCTCTCCTCTGCTTCTGCTTCTCCTTCTTCCTCCCCCGTTTCTTCGCTCGATACTATCGAGCACATTAAAGTCGGCTGTTTCTACCAAATGGACCAATCCAAGCTCCATGGAAGAACAGTACCAGAGCAGCTCAGGGCCGTACGGATCGTCATGGTGAGTGCGAAGGGAGCGTTTAAAGTGGCGGTGAGGTTTCCGAGCCTTCACTCTCTCCACGCTCATATTCCCGCAAAGGGGTATCTAAAACCAGAAGCCAAACAGATTCCGGCGCTCAACGAGAAGTATGTGATGTCCCCGGAAATGGCGTCGGAGGTGCTTTACCGGAGAATCCCACCTCAGGAGATGGTAGATCGGAGCAGCATTTGGAGCTTCTGGGCCGTTCAACCTCCGTTGAAGAATAACCCGAGAAGCACGTTGTCGAGCCCAGCAGTAAGTGGCGGAGGCGGAGCCATTGCTAGCAGCGTGGTTCCTAAAAAGGGTCCGTGTTGGTCCGAGCTCACGTTCGCCGGAATGGTGAAGTGGGGCAAGCGCCGGCAAATTCGCTACCTCCGCAGGCACGAGTCGTCACCGAGCTCTGGCTCTGACGATGAAGAAACGGAGAAGGGAAAGGAGGTAATGGAGCTGGAGGAAGATAGCGATGATGGTTTCACTGAGAAAGTAGTAACTGTGATAGACGAAGACGATGGCATTGAAGAAGAAGAAGAAGGTGAGGAGACAGAGGAAGAAGCTGCTCCTCATGTTCTGCCATGGAAGATGAACCTCCGGAGTCGGAAGCGGAAGCGTCCCGATAATAAGAGAAAAATCAAATCTCCAACCCAAAAGCGATCAAAATCCAGAGCAAAGCCTCCGAATCAGAATAACCATATTGTAGCAGTTCCTGATCGCAGCAGCAGAAAATTGGCTAAAGTTAAACACACAGTAACAAGATGGTCAGCAGGAAGGTACAAGCTGGCGGAGGAGAACATGTTGAAGGTGATGAAGGAGAAAGGGGCGGCGATTGGGAACCCAATACGGCGGCCGGCGCTGAGAGCGGAGGCTCGGAGGCTGATTGGAGACACGGGGTTACTGGATCATTTACTCAAACACATGGCGGGCAAGGTGGCGCCCGGCGGGACGGAGAGATTTAGGCGGCGGCACAACGCCGATGGGGCCATGGAGTATTGGCTGGAGAGTGCTGATCTGGTCCACATCCGGAAGGAGGCTGGGGTTCAAGATCCTTACTGGACCCCTCCGCCGGGGTGGAAGCCCGGCGATAGTCCCACCCAGGACCCCACTTGTGCAAGAGAGATTAAGAAGCTCCGTGAAGAAATTGACAAGATTAGAAGGGAGATGAAGGAGTCCAAGCAGCAAGGGAATGCTGGTTTGGCTATGGTGACCGCTTCAAATTCTAATTTGACCGGTGTTGACTTGGATCGTGACGGTTCATTAATCCAGATTAAGGAGGCCTGTGCAGAGGTAGAGAAGAAGATAGCTAAGATGGATGAAGAAAGGATGGAGTTTGTGACCAAAAAAGAAGAACAAATGGTGGAGGTTACTCAATCTTTGTCCGGAATTAAGGAGAAAATCAGGATGTTCGAATCAGGCAAGGAAGATGCAGTCACAATTTCAGGAGCACCACCACCATCGGAAGCAAAACCAGCCGGTGAGAAGAAAGAAGAAGATAAACTAAGCGGCGGAGACAAGGCGGCTGCGGCGGCGGAGTGCAAGGCGGCAAAAATAGAGAGGCTGAGGAGTGGGTTCAGGATATGCAAGCCGCAAGGCACGTTTCTCTGGCCAGACATGGGCAGCCCCACCAACATTCCTAGTCCTTCTTCCCAGATTCTGGCCCCACCACATGACCTCTTTGTGGTCCCCACCCCACCCTCATCCTCCTCCTCCGCCCCCCGCCCCCTCATCAGCCCCACCACTCCCCCTGTGAAGCCGGTGGCTGAGCGGCGCCCAGTGACCACCACCACATTGTGCAATGTGACCACAATACCCTCTTCAACTCCTATTGTCGCTCCTCCACCTGTCCTGGCCCCAGAGACCCAGAGTTCCACTACCGTCACCATCAGTAAAACCCTACTCATAAACCTCAACGAGCTCCCTGCAACAACAGCACCAAACCCTAACCCTAATCCCCAAACCAGCTGTGAAGCTATCACTACCCTCACCACCTACCACAGAAGACATCACCATCTTCTGCCACGTATGGTGAAGGAGGAGGAGGTGGAGGTGGACGAAACTGCAAGAAACGGTGATGATCAGCAGAGGCGGTGCGCCTCTCCTGCTTCCACGTGGAACGGAGAGAAATGGTGGATGGCTGTGGGAACTCCTAGCACTTCTATGGAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0007049 GO:0008150 GO:0009987 GO:0022414 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0051321
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

804

Amino Acids

88.82

Weight (kDa)

9.12

Isoelectric Point (pI)

66.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 334 - 416 9.6e-26 Winged helix-turn-helix domain in plant reproductive proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1177, 2010
AccB7I CCANNNNNTGG 1 cut(s) 1897
AccBSI CCGCTC 2 cut(s) 1106, 2008
AccIII TCCGGA 3 cut(s) 837, 1266, 1622
AclWI GGATC 4 cut(s) 230, 1152, 1283, 1494
AcoI YGGCCR 3 cut(s) 63, 1091, 1847
AcsI RAATTY 2 cut(s) 612, 1459
AcvI CACGTG 1 cut(s) 2352
AcyI GRCGYC 3 cut(s) 380, 1178, 2011
AdeI CACNNNGTG 1 cut(s) 2034
AfaI GTAC 4 cut(s) 11, 198, 219, 1019
AflIII ACRYGT 1 cut(s) 1038
AgeI ACCGGT 1 cut(s) 1472
AgsI TTSAA 7 cut(s) 458, 469, 767, 1045, 1286, 1458, 2061
AjnI CCWGG 2 cut(s) 1338, 2088
AjuI GAANNNNNNNTTGG 2 cut(s) 1421, 1453
Alw21I GWGCWC 5 cut(s) 141, 573, 650, 1690, 2160
Alw26I GTCTC 4 cut(s) 782, 1122, 1746, 2093
AlwI GGATC 4 cut(s) 230, 1152, 1283, 1494
AlwNI CAGNNNCTG 2 cut(s) 953, 1897
Ama87I CYCGRG 1 cut(s) 478
Aor13HI TCCGGA 3 cut(s) 837, 1266, 1622
AoxI GGCC 9 cut(s) 63, 213, 450, 1091, 1224, 1518, 1847, 1899, 2091
ApoI RAATTY 2 cut(s) 612, 1459
AseI ATTAAT 1 cut(s) 1502
AsiGI ACCGGT 1 cut(s) 1472
AspLEI GCGC 7 cut(s) 76, 346, 606, 1099, 1180, 2013, 2336
AsuC2I CCSGG 4 cut(s) 371, 1182, 1311, 1323
AsuII TTCGAA 1 cut(s) 1650
AsuNHI GCTAGC 1 cut(s) 530
AvaI CYCGRG 1 cut(s) 478
AvaII GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
AxyI CCTNAGG 1 cut(s) 411
BalI TGGCCA 2 cut(s) 65, 1849
BanI GGYRCC 2 cut(s) 1177, 2010
BanII GRGCYC 4 cut(s) 500, 573, 650, 2160
BauI CACGAG 1 cut(s) 632
BbrPI CACGTG 1 cut(s) 2352
BbsI GAAGAC 2 cut(s) 759, 2248
Bbv12I GWGCWC 5 cut(s) 141, 573, 650, 1690, 2160
BbvCI CCTCAGC 1 cut(s) 1802
BceAI ACGGC 3 cut(s) 200, 437, 1103
BcgI CGANNNNNNTGC 2 cut(s) 2144, 2178
BciT130I CCWGG 2 cut(s) 1340, 2090
BclI TGATCA 1 cut(s) 2317
BcnI CCSGG 4 cut(s) 371, 1182, 1311, 1323
BcoDI GTCTC 4 cut(s) 782, 1122, 1746, 2093
BfaI CTAG 3 cut(s) 531, 1878, 2391
BfoI RGCGCY 5 cut(s) 347, 607, 1100, 1181, 2014
BglI GCCNNNNNGGC 1 cut(s) 1223
BlpI GCTNAGC 1 cut(s) 2004
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 6 cut(s) 371, 1182, 1311, 1323, 1340, 2090
Bme18I GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
BmeT110I CYCGRG 1 cut(s) 478
BmrFI CCNGG 6 cut(s) 371, 1182, 1311, 1323, 1340, 2090
BmrI ACTGGG 1 cut(s) 2009
BmsI GCATC 1 cut(s) 1657
BmtI GCTAGC 1 cut(s) 534
BmuI ACTGGG 1 cut(s) 2009
BpiI GAAGAC 2 cut(s) 759, 2248
BpmI CTGGAG 2 cut(s) 719, 1262
Bpu10I CCTNAGC 2 cut(s) 209, 1802
Bpu1102I GCTNAGC 1 cut(s) 2004
Bpu14I TTCGAA 1 cut(s) 1650
BpuMI CCSGG 4 cut(s) 371, 1182, 1311, 1323
BsaAI YACGTR 2 cut(s) 2264, 2352
BsaBI GATNNNNATC 1 cut(s) 904
BsaHI GRCGYC 3 cut(s) 380, 1178, 2011
BsaI GGTCTC 1 cut(s) 2093
BsaJI CCNNGG 6 cut(s) 184, 369, 821, 1227, 1310, 1338
BsaWI WCCGGW 5 cut(s) 396, 837, 1266, 1472, 1622
BsaXI ACNNNNNCTCC 2 cut(s) 1025, 1055
Bse118I RCCGGY 5 cut(s) 606, 1093, 1472, 1715, 1996
Bse1I ACTGG 3 cut(s) 1146, 1301, 2015
Bse21I CCTNAGG 1 cut(s) 411
Bse3DI GCAATG 2 cut(s) 525, 2044
Bse8I GATNNNNATC 1 cut(s) 904
BseAI TCCGGA 3 cut(s) 837, 1266, 1622
BseBI CCWGG 2 cut(s) 1340, 2090
BseDI CCNNGG 6 cut(s) 184, 369, 821, 1227, 1310, 1338
BseGI GGATG 6 cut(s) 1263, 1561, 1572, 1650, 1940, 2379
BseJI GATNNNNATC 1 cut(s) 904
BseMI GCAATG 2 cut(s) 525, 2044
BseMII CTCAG 6 cut(s) 223, 425, 717, 1091, 1793, 1995
BseNI ACTGG 3 cut(s) 1146, 1301, 2015
BseX3I CGGCCG 1 cut(s) 1091
BseYI CCCAGC 2 cut(s) 499, 1277
BsgI GTGCAG 1 cut(s) 1545
Bsh1285I CGRYCG 1 cut(s) 1094
BshFI GGCC 9 cut(s) 65, 215, 452, 1093, 1226, 1520, 1849, 1901, 2093
BshNI GGYRCC 2 cut(s) 1177, 2010
BshTI ACCGGT 1 cut(s) 1472
BsiEI CGRYCG 1 cut(s) 1094
BsiHKAI GWGCWC 5 cut(s) 141, 573, 650, 1690, 2160
BsiHKCI CYCGRG 1 cut(s) 478
BsiWI CGTACG 1 cut(s) 217
BslFI GGGAC 5 cut(s) 352, 843, 1201, 1317, 1910
BsmAI GTCTC 4 cut(s) 782, 1122, 1746, 2093
BsmFI GGGAC 5 cut(s) 352, 843, 1201, 1317, 1910
BsmI GAATGC 2 cut(s) 50, 1435
BsnI GGCC 9 cut(s) 65, 215, 452, 1093, 1226, 1520, 1849, 1901, 2093
Bso31I GGTCTC 1 cut(s) 2093
BsoBI CYCGRG 1 cut(s) 478
Bsp119I TTCGAA 1 cut(s) 1650
Bsp1286I GDGCHC 6 cut(s) 141, 500, 573, 650, 1690, 2160
Bsp13I TCCGGA 3 cut(s) 837, 1266, 1622
Bsp143I GATC 9 cut(s) 222, 424, 899, 955, 1144, 1252, 1288, 1486, 2317
Bsp1720I GCTNAGC 1 cut(s) 2004
Bsp19I CCATGG 3 cut(s) 184, 821, 1227
BspANI GGCC 9 cut(s) 65, 215, 452, 1093, 1226, 1520, 1849, 1901, 2093
BspCNI CTCAG 6 cut(s) 222, 424, 718, 1092, 1794, 1996
BspEI TCCGGA 3 cut(s) 837, 1266, 1622
BspOI GCTAGC 1 cut(s) 534
BspPI GGATC 4 cut(s) 230, 1152, 1283, 1494
BspT104I TTCGAA 1 cut(s) 1650
BspT107I GGYRCC 2 cut(s) 1177, 2010
BspTNI GGTCTC 1 cut(s) 2093
BsrBI CCGCTC 2 cut(s) 1106, 2008
BsrDI GCAATG 2 cut(s) 525, 2044
BsrFI RCCGGY 5 cut(s) 606, 1093, 1472, 1715, 1996
BsrI ACTGG 3 cut(s) 1146, 1301, 2015
BssAI RCCGGY 5 cut(s) 606, 1093, 1472, 1715, 1996
BssECI CCNNGG 6 cut(s) 184, 369, 821, 1227, 1310, 1338
BssMI GATC 9 cut(s) 222, 424, 899, 955, 1144, 1252, 1288, 1486, 2317
BssNI GRCGYC 3 cut(s) 380, 1178, 2011
BssSI CACGAG 1 cut(s) 632
BssT1I CCWWGG 3 cut(s) 184, 821, 1227
Bst2BI CACGAG 1 cut(s) 632
Bst2UI CCWGG 2 cut(s) 1340, 2090
Bst4CI ACNGT 6 cut(s) 196, 742, 994, 1496, 2119, 2312
Bst6I CTCTTC 1 cut(s) 2062
BstACI GRCGYC 3 cut(s) 380, 1178, 2011
BstBAI YACGTR 2 cut(s) 2264, 2352
BstBI TTCGAA 1 cut(s) 1650
BstC8I GCNNGC 8 cut(s) 532, 602, 608, 629, 1026, 1095, 1168, 1826
BstDEI CTNAG 8 cut(s) 209, 411, 726, 1100, 1548, 1742, 1802, 2004
BstDSI CCRYGG 3 cut(s) 184, 821, 1227
BstEII GGTNACC 1 cut(s) 1447
BstF5I GGATG 6 cut(s) 1263, 1561, 1572, 1650, 1940, 2379
BstH2I RGCGCY 5 cut(s) 347, 607, 1100, 1181, 2014
BstHHI GCGC 7 cut(s) 76, 346, 606, 1099, 1180, 2013, 2336
BstKTI GATC 9 cut(s) 225, 427, 902, 958, 1147, 1255, 1291, 1489, 2320
BstMAI GTCTC 4 cut(s) 782, 1122, 1746, 2093
BstMBI GATC 9 cut(s) 222, 424, 899, 955, 1144, 1252, 1288, 1486, 2317
BstMCI CGRYCG 1 cut(s) 1094
BstNI CCWGG 2 cut(s) 1340, 2090
BstNSI RCATGY 1 cut(s) 1042
BstPI GGTNACC 1 cut(s) 1447
BstSCI CCNGG 6 cut(s) 369, 1180, 1309, 1321, 1338, 2088
BstV2I GAAGAC 2 cut(s) 759, 2248
BstX2I RGATCY 1 cut(s) 1288
BstYI RGATCY 1 cut(s) 1288
BstZI CGGCCG 1 cut(s) 1091
Bsu36I CCTNAGG 1 cut(s) 411
BsuRI GGCC 9 cut(s) 65, 215, 452, 1093, 1226, 1520, 1849, 1901, 2093
BtgI CCRYGG 3 cut(s) 184, 821, 1227
BtgZI GCGATG 1 cut(s) 726
BtsCI GGATG 6 cut(s) 1263, 1561, 1572, 1650, 1940, 2379
BtsIMutI CAGTG 2 cut(s) 723, 2022
Cac8I GCNNGC 8 cut(s) 532, 602, 608, 629, 1026, 1095, 1168, 1826
CaiI CAGNNNCTG 2 cut(s) 953, 1897
CfoI GCGC 7 cut(s) 76, 346, 606, 1099, 1180, 2013, 2336
Cfr10I RCCGGY 5 cut(s) 606, 1093, 1472, 1715, 1996
CseI GACGC 2 cut(s) 369, 845
Csp6I GTAC 4 cut(s) 10, 197, 218, 1018
CspAI ACCGGT 1 cut(s) 1472
CviAII CATG 9 cut(s) 185, 229, 812, 822, 1039, 1162, 1228, 1855, 1910
CviQI GTAC 4 cut(s) 10, 197, 218, 1018
DdeI CTNAG 8 cut(s) 209, 411, 726, 1100, 1548, 1742, 1802, 2004
DinI GGCGCC 2 cut(s) 1179, 2012
DpnI GATC 9 cut(s) 224, 426, 901, 957, 1146, 1254, 1290, 1488, 2319
DpnII GATC 9 cut(s) 222, 424, 899, 955, 1144, 1252, 1288, 1486, 2317
DraI TTTAAA 1 cut(s) 253
DraIII CACNNNGTG 1 cut(s) 2034
EaeI YGGCCR 3 cut(s) 63, 1091, 1847
EagI CGGCCG 1 cut(s) 1091
Eam1104I CTCTTC 1 cut(s) 2062
EarI CTCTTC 1 cut(s) 2062
EciI GGCGGA 7 cut(s) 528, 534, 1043, 1296, 1764, 1787, 1942
Ecl136II GAGCTC 3 cut(s) 571, 648, 2158
EclXI CGGCCG 1 cut(s) 1091
Eco130I CCWWGG 3 cut(s) 184, 821, 1227
Eco147I AGGCCT 1 cut(s) 1520
Eco24I GRGCYC 4 cut(s) 500, 573, 650, 2160
Eco31I GGTCTC 1 cut(s) 2093
Eco47I GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
Eco52I CGGCCG 1 cut(s) 1091
Eco53kI GAGCTC 3 cut(s) 571, 648, 2158
Eco72I CACGTG 1 cut(s) 2352
Eco81I CCTNAGG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 478
Eco91I GGTNACC 1 cut(s) 1447
EcoICRI GAGCTC 3 cut(s) 571, 648, 2158
EcoO109I RGGNCCY 1 cut(s) 1342
EcoO65I GGTNACC 1 cut(s) 1447
EcoRII CCWGG 2 cut(s) 1338, 2088
EcoT14I CCWWGG 3 cut(s) 184, 821, 1227
EcoT38I GRGCYC 4 cut(s) 500, 573, 650, 2160
EgeI GGCGCC 2 cut(s) 1179, 2012
EheI GGCGCC 2 cut(s) 1179, 2012
ErhI CCWWGG 3 cut(s) 184, 821, 1227
FaeI CATG 9 cut(s) 188, 232, 815, 825, 1042, 1165, 1231, 1858, 1913
FaqI GGGAC 5 cut(s) 352, 843, 1201, 1317, 1910
FatI CATG 9 cut(s) 184, 228, 811, 821, 1038, 1161, 1227, 1854, 1909
FauI CCCGC 4 cut(s) 310, 1159, 1178, 1967
FauNDI CATATG 1 cut(s) 70
FbaI TGATCA 1 cut(s) 2317
FokI GGATG 6 cut(s) 1250, 1568, 1579, 1657, 1927, 2386
FriOI GRGCYC 4 cut(s) 500, 573, 650, 2160
FspBI CTAG 3 cut(s) 531, 1878, 2391
GlaI GCGC 7 cut(s) 75, 345, 605, 1098, 1179, 2012, 2335
GsaI CCCAGC 2 cut(s) 503, 1281
GsuI CTGGAG 2 cut(s) 719, 1262
HaeII RGCGCY 5 cut(s) 347, 607, 1100, 1181, 2014
HaeIII GGCC 9 cut(s) 65, 215, 452, 1093, 1226, 1520, 1849, 1901, 2093
HgaI GACGC 2 cut(s) 369, 845
HhaI GCGC 7 cut(s) 76, 346, 606, 1099, 1180, 2013, 2336
Hin1I GRCGYC 3 cut(s) 380, 1178, 2011
Hin1II CATG 9 cut(s) 188, 232, 815, 825, 1042, 1165, 1231, 1858, 1913
Hin6I GCGC 7 cut(s) 74, 344, 604, 1097, 1178, 2011, 2334
HinP1I GCGC 7 cut(s) 74, 344, 604, 1097, 1178, 2011, 2334
HincII GTYRAC 1 cut(s) 1480
HindII GTYRAC 1 cut(s) 1480
HinfI GANTC 8 cut(s) 337, 402, 635, 841, 925, 1412, 1652, 1894
Hpy166II GTNNAC 3 cut(s) 1261, 1480, 2293
Hpy8I GTNNAC 3 cut(s) 1261, 1480, 2293
Hpy99I CGWCG 1 cut(s) 385
HpyCH4III ACNGT 6 cut(s) 196, 742, 994, 1496, 2119, 2312
HpyCH4IV ACGT 5 cut(s) 488, 575, 1838, 2263, 2351
HpyCH4V TGCA 9 cut(s) 48, 1355, 1526, 1670, 1779, 1824, 2037, 2165, 2303
HpyF3I CTNAG 8 cut(s) 209, 411, 726, 1100, 1548, 1742, 1802, 2004
HpySE526I ACGT 5 cut(s) 488, 575, 1838, 2263, 2351
Hsp92I GRCGYC 3 cut(s) 380, 1178, 2011
Hsp92II CATG 9 cut(s) 188, 232, 815, 825, 1042, 1165, 1231, 1858, 1913
HspAI GCGC 7 cut(s) 74, 344, 604, 1097, 1178, 2011, 2334
KasI GGCGCC 2 cut(s) 1177, 2010
Kpn2I TCCGGA 3 cut(s) 837, 1266, 1622
KroI GCCGGC 2 cut(s) 606, 1093
KroNI GCCGGC 2 cut(s) 608, 1095
Ksp22I TGATCA 1 cut(s) 2317
Kzo9I GATC 9 cut(s) 222, 424, 899, 955, 1144, 1252, 1288, 1486, 2317
LweI GCATC 1 cut(s) 1657
MaeI CTAG 3 cut(s) 531, 1878, 2391
MaeII ACGT 5 cut(s) 488, 575, 1838, 2263, 2351
MalI GATC 9 cut(s) 224, 426, 901, 957, 1146, 1254, 1290, 1488, 2319
MbiI CCGCTC 2 cut(s) 1106, 2008
MboI GATC 9 cut(s) 222, 424, 899, 955, 1144, 1252, 1288, 1486, 2317
MflI RGATCY 1 cut(s) 1288
MhlI GDGCHC 6 cut(s) 141, 500, 573, 650, 1690, 2160
MlsI TGGCCA 2 cut(s) 65, 1849
MluCI AATT 7 cut(s) 612, 971, 1383, 1459, 1465, 1626, 1677
MluNI TGGCCA 2 cut(s) 65, 1849
Mly113I GGCGCC 2 cut(s) 1178, 2011
MlyI GAGTC 3 cut(s) 644, 850, 1421
MmeI TCCRAC 3 cut(s) 363, 824, 911
Mox20I TGGCCA 2 cut(s) 65, 1849
MroI TCCGGA 3 cut(s) 837, 1266, 1622
MroNI GCCGGC 2 cut(s) 606, 1093
MscI TGGCCA 2 cut(s) 65, 1849
MseI TTAA 7 cut(s) 144, 252, 984, 1365, 1502, 1512, 1629
MslI CAYNNNNRTG 1 cut(s) 2399
Msp20I TGGCCA 2 cut(s) 65, 1849
MspA1I CMGCKG 1 cut(s) 2205
MspR9I CCNGG 6 cut(s) 371, 1182, 1311, 1323, 1340, 2090
Mva1269I GAATGC 2 cut(s) 50, 1435
MvaI CCWGG 2 cut(s) 1340, 2090
NaeI GCCGGC 2 cut(s) 608, 1095
NarI GGCGCC 2 cut(s) 1178, 2011
NciI CCSGG 4 cut(s) 371, 1182, 1311, 1323
NcoI CCATGG 3 cut(s) 184, 821, 1227
NdeI CATATG 1 cut(s) 70
NdeII GATC 9 cut(s) 222, 424, 899, 955, 1144, 1252, 1288, 1486, 2317
NgoMIV GCCGGC 2 cut(s) 606, 1093
NheI GCTAGC 1 cut(s) 530
NlaIII CATG 9 cut(s) 188, 232, 815, 825, 1042, 1165, 1231, 1858, 1913
NmuCI GTSAC 8 cut(s) 639, 1447, 1490, 1576, 1672, 2017, 2041, 2119
NspI RCATGY 1 cut(s) 1042
NspV TTCGAA 1 cut(s) 1650
PceI AGGCCT 1 cut(s) 1520
PciI ACATGT 1 cut(s) 1038
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PctI GAATGC 2 cut(s) 50, 1435
PdiI GCCGGC 2 cut(s) 608, 1095
PfeI GAWTC 5 cut(s) 337, 402, 925, 1652, 1894
Pfl23II CGTACG 1 cut(s) 217
PflMI CCANNNNNTGG 1 cut(s) 1897
PinAI ACCGGT 1 cut(s) 1472
PleI GAGTC 3 cut(s) 643, 849, 1420
PluTI GGCGCC 2 cut(s) 1181, 2014
PmaCI CACGTG 1 cut(s) 2352
PmlI CACGTG 1 cut(s) 2352
PpsI GAGTC 3 cut(s) 643, 849, 1420
Ppu21I YACGTR 2 cut(s) 2264, 2352
PpuMI RGGWCCY 1 cut(s) 1342
PscI ACATGT 1 cut(s) 1038
PshBI ATTAAT 1 cut(s) 1502
Psp124BI GAGCTC 3 cut(s) 573, 650, 2160
Psp5II RGGWCCY 1 cut(s) 1342
Psp6I CCWGG 2 cut(s) 1338, 2088
PspCI CACGTG 1 cut(s) 2352
PspEI GGTNACC 1 cut(s) 1447
PspFI CCCAGC 2 cut(s) 499, 1277
PspGI CCWGG 2 cut(s) 1338, 2088
PspLI CGTACG 1 cut(s) 217
PspPPI RGGWCCY 1 cut(s) 1342
PstNI CAGNNNCTG 2 cut(s) 953, 1897
PsuI RGATCY 1 cut(s) 1288
PvuII CAGCTG 1 cut(s) 2205
RsaI GTAC 4 cut(s) 11, 198, 219, 1019
RsaNI GTAC 4 cut(s) 10, 197, 218, 1018
RseI CAYNNNNRTG 1 cut(s) 2399
SacI GAGCTC 3 cut(s) 573, 650, 2160
SaqAI TTAA 7 cut(s) 144, 252, 984, 1365, 1502, 1512, 1629
Sau3AI GATC 9 cut(s) 222, 424, 899, 955, 1144, 1252, 1288, 1486, 2317
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 3 cut(s) 644, 850, 1421
ScrFI CCNGG 6 cut(s) 371, 1182, 1311, 1323, 1340, 2090
SduI GDGCHC 6 cut(s) 141, 500, 573, 650, 1690, 2160
SfaNI GCATC 1 cut(s) 1657
SfoI GGCGCC 2 cut(s) 1179, 2012
SfuI TTCGAA 1 cut(s) 1650
SinI GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
SmiMI CAYNNNNRTG 1 cut(s) 2399
Sse9I AATT 7 cut(s) 612, 971, 1383, 1459, 1465, 1626, 1677
SseBI AGGCCT 1 cut(s) 1520
SspDI GGCGCC 2 cut(s) 1177, 2010
SspMI CTAG 3 cut(s) 531, 1878, 2391
SstI GAGCTC 3 cut(s) 573, 650, 2160
StuI AGGCCT 1 cut(s) 1520
StyD4I CCNGG 6 cut(s) 369, 1180, 1309, 1321, 1338, 2088
StyI CCWWGG 3 cut(s) 184, 821, 1227
TaaI ACNGT 6 cut(s) 196, 742, 994, 1496, 2119, 2312
TaiI ACGT 5 cut(s) 491, 578, 1841, 2266, 2354
TaqI TCGA 4 cut(s) 126, 135, 494, 1650
TasI AATT 7 cut(s) 612, 971, 1383, 1459, 1465, 1626, 1677
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 5 cut(s) 337, 402, 925, 1652, 1894
Tru1I TTAA 7 cut(s) 144, 252, 984, 1365, 1502, 1512, 1629
Tru9I TTAA 7 cut(s) 144, 252, 984, 1365, 1502, 1512, 1629
TscAI CASTG 2 cut(s) 730, 2022
TseFI GTSAC 8 cut(s) 639, 1447, 1490, 1576, 1672, 2017, 2041, 2119
Tsp45I GTSAC 8 cut(s) 639, 1447, 1490, 1576, 1672, 2017, 2041, 2119
TspDTI ATGAA 6 cut(s) 678, 845, 1067, 1421, 1488, 1572
TspGWI ACGGA 7 cut(s) 235, 453, 546, 686, 1205, 1364, 2373
TspRI CASTG 2 cut(s) 730, 2022
Van91I CCANNNNNTGG 1 cut(s) 1897
VpaK11BI GGWCC 7 cut(s) 169, 554, 564, 1258, 1299, 1342, 1924
VspI ATTAAT 1 cut(s) 1502
XapI RAATTY 2 cut(s) 612, 1459
XceI RCATGY 1 cut(s) 1042
XspI CTAG 3 cut(s) 531, 1878, 2391
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.