Rh1AG390500

DYAD-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001779
Physical Location & Seq
Forward (+)
61985121 .. 61988703
3583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG390500.1

Sequence Viewer

Length: 1275 bp
ATGAAGAAGTACTTGGAGAACAAGCATCAAGAACAAAGAGCACAAAGCCTTTGTTTGGCAACACATGCTTCTGCTTCTCAAGAGACCATCGACCACATCCATGTGGGTTCATTCTATGAACTAGACCATTTAAAGCTTCCTGCTACTGCCTCAGAAGAGATGAAGTCGATTCGGGTCGTCATGGTGAATGGAAAGTCTCTGTTCAAGGTTTCAGTGAGCTTTCCAAGCCTTCACTCTCTCCAAACCCATCTGAATGAGGAAAGCTACCAAAACCCAGACGGCTTCCTTCCTGCTTTCGACGAAAACTACTCTATGGCTCCACAAATCGCGTCGGAGGTGCTTTACCGGAGAATCCCACATCACGAAATAGCAGAGCAGAGCATGATGTGGAGTTTTTGGGCCGGACGGCGTCGTTTCACTACCCGGAAAAGCCCATCTCCGTGTTGCTTGTCGGAGATTAAGCTCGGCGGCATGGTCCAATGGGGTAAGCGCAAACTAGTCCGGTGCGTTACGAGGCATGAGAGTCTGTCGAGCTCTGACCAAGAACAACCAGAGAAGGAAGAAACGGAGATGGATGAAGATGTGAAAGAGCCTCGCTATGAGTTTAGGAAGAGAAAACTGGTTCAAAAGAAGACCAAAGCAGCGAAGCGTCGGAAGCAGAACAATCAAATAGTGGTTCATGACCGGACCCAGAATCAATTGGTTGAAGCCTCAATAGAGAGATGGACTGCAAGGAGGTATAAGGCAGCAGAGGAGAACATACTTAAGGTGATGAAGGCAAAAGGAGTTTGTGCTGAGAATCCAATACGGAGAGCACCACTGAGGTGTGAGGCCCGGAAGTTGATTGGGGACACTGGTTTGATAGACCATTTGTTGAAGCATATGGCTGGGAAGGTAGCTCCGGGTGGCGCTGATCGGTTTCGGAGAAGGCATAATGCTGATGGAGCACTTGTGTATTGGCTGGAGAGTGCTGATTTAATGAGTGTAAGGAAGGAGGCTGGGGTTGAGGATCCTTATTGGACTCCACCCCTGGGGTGGAAGATTGGGGATAACCCAACTCAGGACCCTACTTGTGTGAGGATGTTCAAGGAGATTTGGGAAGAGATAGCCAATGTGAAAAGAGAGAGGGAATTTCAGCAGCAAGAGGATATAGCTATGGTGACCACCTCAAACTCTTCGCTGACAACTCTGGATCAGGGCCAAGCTTACAGTTCAGTAACCAATCGAAAGGTAAATGATGAGAAGCGATGCAAATCAAATGTATGGAATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0007049 GO:0008150 GO:0009987 GO:0022414 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044424 GO:0044464 GO:0051321
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

424

Amino Acids

48.97

Weight (kDa)

9.2

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 241 - 323 4.3e-24 Winged helix-turn-helix domain in plant reproductive proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 329
AciI CCGC 1 cut(s) 468
AclWI GGATC 3 cut(s) 1004, 1017, 1200
AcsI RAATTY 1 cut(s) 1130
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 4 cut(s) 55, 1031, 1035, 1060
AflII CTTAAG 1 cut(s) 764
AgsI TTSAA 5 cut(s) 205, 626, 707, 877, 1087
AhlI ACTAGT 1 cut(s) 496
AjnI CCWGG 1 cut(s) 1029
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AleI CACNNNNGTG 1 cut(s) 823
AluBI AGCT 8 cut(s) 136, 219, 264, 463, 534, 899, 1154, 1205
AluI AGCT 8 cut(s) 136, 219, 264, 463, 534, 899, 1154, 1205
Alw21I GWGCWC 4 cut(s) 43, 536, 817, 949
Alw26I GTCTC 2 cut(s) 77, 201
AlwI GGATC 3 cut(s) 1004, 1017, 1200
AoxI GGCC 3 cut(s) 399, 831, 1198
ApeKI GCWGC 3 cut(s) 641, 746, 1138
ApoI RAATTY 1 cut(s) 1130
AspLEI GCGC 2 cut(s) 492, 911
AspS9I GGNCC 6 cut(s) 399, 475, 687, 832, 1063, 1198
AsuC2I CCSGG 3 cut(s) 424, 835, 903
AsuHPI GGTGA 3 cut(s) 196, 781, 1171
AvaII GGWCC 3 cut(s) 475, 687, 1063
BamHI GGATCC 1 cut(s) 1009
BanII GRGCYC 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 638
Bbv12I GWGCWC 4 cut(s) 43, 536, 817, 949
BbvI GCAGC 3 cut(s) 653, 758, 1150
BccI CCATC 6 cut(s) 95, 255, 442, 565, 717, 935
BceAI ACGGC 2 cut(s) 295, 422
BciT130I CCWGG 1 cut(s) 1031
BcnI CCSGG 3 cut(s) 424, 835, 903
BcoDI GTCTC 2 cut(s) 77, 201
BcuI ACTAGT 1 cut(s) 496
BfaI CTAG 2 cut(s) 122, 497
BfoI RGCGCY 1 cut(s) 912
BfrI CTTAAG 1 cut(s) 764
BisI GCNGC 4 cut(s) 469, 642, 747, 1139
BlsI GCNGC 4 cut(s) 470, 643, 748, 1140
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 4 cut(s) 424, 835, 903, 1031
Bme18I GGWCC 3 cut(s) 475, 687, 1063
BmgT120I GGNCC 6 cut(s) 399, 475, 687, 832, 1063, 1198
BmiI GGNNCC 4 cut(s) 318, 689, 1011, 1065
BmrFI CCNGG 4 cut(s) 424, 835, 903, 1031
BmsI GCATC 2 cut(s) 34, 1238
BpiI GAAGAC 1 cut(s) 638
BpmI CTGGAG 1 cut(s) 983
BpuEI CTTGAG 1 cut(s) 63
BpuMI CCSGG 3 cut(s) 424, 835, 903
BsaBI GATNNNNATC 1 cut(s) 1252
BsaHI GRCGYC 1 cut(s) 409
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 1029, 1030
BsaWI WCCGGW 3 cut(s) 345, 501, 684
BsaXI ACNNNNNCTCC 2 cut(s) 936, 966
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 1031, 1035, 1060
Bse1I ACTGG 2 cut(s) 624, 859
Bse8I GATNNNNATC 1 cut(s) 1252
BseBI CCWGG 1 cut(s) 1031
BseDI CCNNGG 2 cut(s) 1029, 1030
BseGI GGATG 3 cut(s) 96, 580, 1086
BseJI GATNNNNATC 1 cut(s) 1252
BseLI CCNNNNNNNGG 4 cut(s) 55, 1031, 1035, 1060
BseMII CTCAG 4 cut(s) 165, 786, 812, 1073
BseNI ACTGG 2 cut(s) 624, 859
BseRI GAGGAG 1 cut(s) 767
BseXI GCAGC 3 cut(s) 653, 758, 1150
BseYI CCCAGC 2 cut(s) 887, 998
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 3 cut(s) 401, 833, 1200
BsiHKAI GWGCWC 4 cut(s) 43, 536, 817, 949
BsiSI CCGG 7 cut(s) 346, 402, 424, 502, 685, 835, 902
BslFI GGGAC 1 cut(s) 863
BslI CCNNNNNNNGG 4 cut(s) 55, 1031, 1035, 1060
BsmAI GTCTC 2 cut(s) 77, 201
BsmFI GGGAC 1 cut(s) 863
BsnI GGCC 3 cut(s) 401, 833, 1200
Bso31I GGTCTC 1 cut(s) 77
Bsp1286I GDGCHC 4 cut(s) 43, 536, 817, 949
Bsp143I GATC 3 cut(s) 913, 1009, 1192
BspACI CCGC 1 cut(s) 468
BspANI GGCC 3 cut(s) 401, 833, 1200
BspCNI CTCAG 4 cut(s) 164, 787, 813, 1072
BspFNI CGCG 1 cut(s) 329
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 4 cut(s) 318, 689, 1011, 1065
BspPI GGATC 3 cut(s) 1004, 1017, 1200
BspTI CTTAAG 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 77
BsrI ACTGG 2 cut(s) 624, 859
BssECI CCNNGG 2 cut(s) 1029, 1030
BssMI GATC 3 cut(s) 913, 1009, 1192
BssNI GRCGYC 1 cut(s) 409
Bst2UI CCWGG 1 cut(s) 1031
Bst4CI ACNGT 1 cut(s) 1211
Bst6I CTCTTC 4 cut(s) 150, 605, 1095, 1180
BstACI GRCGYC 1 cut(s) 409
BstAFI CTTAAG 1 cut(s) 764
BstAPI GCANNNNNTGC 1 cut(s) 65
BstDEI CTNAG 4 cut(s) 151, 795, 821, 1059
BstEII GGTNACC 1 cut(s) 1159
BstF5I GGATG 3 cut(s) 96, 580, 1086
BstFNI CGCG 1 cut(s) 329
BstH2I RGCGCY 1 cut(s) 912
BstHHI GCGC 2 cut(s) 492, 911
BstKTI GATC 3 cut(s) 916, 1012, 1195
BstMAI GTCTC 2 cut(s) 77, 201
BstMBI GATC 3 cut(s) 913, 1009, 1192
BstMWI GCNNNNNNNGC 4 cut(s) 65, 225, 655, 944
BstNI CCWGG 1 cut(s) 1031
BstNSI RCATGY 1 cut(s) 68
BstPI GGTNACC 1 cut(s) 1159
BstSCI CCNGG 4 cut(s) 422, 833, 901, 1029
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 3 cut(s) 653, 758, 1150
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BsuRI GGCC 3 cut(s) 401, 833, 1200
BtgZI GCGATG 1 cut(s) 1261
BtsCI GGATG 3 cut(s) 96, 580, 1086
BtsIMutI CAGTG 3 cut(s) 219, 818, 852
CciI TCATGA 1 cut(s) 679
CfoI GCGC 2 cut(s) 492, 911
Cfr13I GGNCC 6 cut(s) 399, 475, 687, 832, 1063, 1198
CseI GACGC 3 cut(s) 318, 398, 638
Csp6I GTAC 1 cut(s) 10
CviAII CATG 7 cut(s) 65, 101, 181, 382, 472, 518, 680
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 4 cut(s) 151, 795, 821, 1059
DpnI GATC 3 cut(s) 915, 1011, 1194
DpnII GATC 3 cut(s) 913, 1009, 1192
DraI TTTAAA 1 cut(s) 132
Eam1104I CTCTTC 4 cut(s) 150, 605, 1095, 1180
EarI CTCTTC 4 cut(s) 150, 605, 1095, 1180
Ecl136II GAGCTC 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 536
Eco31I GGTCTC 1 cut(s) 77
Eco47I GGWCC 3 cut(s) 475, 687, 1063
Eco53kI GAGCTC 1 cut(s) 534
Eco91I GGTNACC 1 cut(s) 1159
EcoICRI GAGCTC 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 1063
EcoO65I GGTNACC 1 cut(s) 1159
EcoRII CCWGG 1 cut(s) 1029
EcoT38I GRGCYC 1 cut(s) 536
FaeI CATG 7 cut(s) 68, 104, 184, 385, 475, 521, 683
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 1 cut(s) 863
FatI CATG 7 cut(s) 64, 100, 180, 381, 471, 517, 679
FauNDI CATATG 1 cut(s) 882
Fnu4HI GCNGC 4 cut(s) 469, 642, 747, 1139
FokI GGATG 3 cut(s) 83, 587, 1093
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 4 cut(s) 469, 642, 747, 1139
FspBI CTAG 2 cut(s) 122, 497
GlaI GCGC 2 cut(s) 491, 910
GluI GCNGC 4 cut(s) 469, 642, 747, 1139
GsaI CCCAGC 2 cut(s) 891, 1002
GsuI CTGGAG 1 cut(s) 983
HaeII RGCGCY 1 cut(s) 912
HaeIII GGCC 3 cut(s) 401, 833, 1200
HapII CCGG 7 cut(s) 346, 402, 424, 502, 685, 835, 902
HgaI GACGC 3 cut(s) 318, 398, 638
HhaI GCGC 2 cut(s) 492, 911
Hin1I GRCGYC 1 cut(s) 409
Hin1II CATG 7 cut(s) 68, 104, 184, 385, 475, 521, 683
Hin6I GCGC 2 cut(s) 490, 909
HinP1I GCGC 2 cut(s) 490, 909
HindIII AAGCTT 2 cut(s) 134, 1203
HinfI GANTC 6 cut(s) 169, 351, 523, 694, 799, 1021
HpaII CCGG 7 cut(s) 346, 402, 424, 502, 685, 835, 902
HphI GGTGA 3 cut(s) 196, 781, 1171
Hpy188I TCNGA 7 cut(s) 154, 252, 334, 454, 538, 654, 924
Hpy188III TCNNGA 6 cut(s) 29, 80, 362, 680, 1061, 1190
Hpy99I CGWCG 4 cut(s) 302, 334, 414, 654
HpyAV CCTTC 7 cut(s) 239, 296, 550, 769, 886, 921, 985
HpyCH4III ACNGT 1 cut(s) 1211
HpyCH4V TGCA 2 cut(s) 731, 1251
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 225, 655, 944
HpyF3I CTNAG 4 cut(s) 151, 795, 821, 1059
Hsp92I GRCGYC 1 cut(s) 409
Hsp92II CATG 7 cut(s) 68, 104, 184, 385, 475, 521, 683
HspAI GCGC 2 cut(s) 490, 909
Kzo9I GATC 3 cut(s) 913, 1009, 1192
LmnI GCTCC 3 cut(s) 322, 904, 944
Lsp1109I GCAGC 3 cut(s) 653, 758, 1150
LweI GCATC 2 cut(s) 34, 1238
MaeI CTAG 2 cut(s) 122, 497
MaeIII GTNAC 3 cut(s) 508, 1159, 1216
MalI GATC 3 cut(s) 915, 1011, 1194
MboI GATC 3 cut(s) 913, 1009, 1192
MboII GAAGA 9 cut(s) 16, 167, 572, 590, 622, 643, 1051, 1112, 1167
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 4 cut(s) 43, 536, 817, 949
MluCI AATT 2 cut(s) 698, 1130
MlyI GAGTC 2 cut(s) 532, 1015
MmeI TCCRAC 3 cut(s) 312, 432, 632
MseI TTAA 4 cut(s) 131, 459, 765, 977
MslI CAYNNNNRTG 5 cut(s) 99, 101, 252, 439, 823
MspCI CTTAAG 1 cut(s) 764
MspI CCGG 7 cut(s) 346, 402, 424, 502, 685, 835, 902
MspR9I CCNGG 4 cut(s) 424, 835, 903, 1031
MunI CAATTG 1 cut(s) 698
MvaI CCWGG 1 cut(s) 1031
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 4 cut(s) 65, 225, 655, 944
NciI CCSGG 3 cut(s) 424, 835, 903
NdeI CATATG 1 cut(s) 882
NdeII GATC 3 cut(s) 913, 1009, 1192
NlaIII CATG 7 cut(s) 68, 104, 184, 385, 475, 521, 683
NlaIV GGNNCC 4 cut(s) 318, 689, 1011, 1065
NmeAIII GCCGAG 1 cut(s) 444
NmuCI GTSAC 1 cut(s) 1159
NspI RCATGY 1 cut(s) 68
OliI CACNNNNGTG 1 cut(s) 823
PagI TCATGA 1 cut(s) 679
PasI CCCWGGG 1 cut(s) 1030
PfeI GAWTC 4 cut(s) 169, 351, 694, 799
PflFI GACNNNGTC 1 cut(s) 408
PkrI GCNGC 4 cut(s) 470, 643, 748, 1140
PleI GAGTC 2 cut(s) 531, 1015
PpsI GAGTC 2 cut(s) 531, 1015
PpuMI RGGWCCY 1 cut(s) 1063
Psp124BI GAGCTC 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 1063
Psp6I CCWGG 1 cut(s) 1029
PspEI GGTNACC 1 cut(s) 1159
PspFI CCCAGC 2 cut(s) 887, 998
PspGI CCWGG 1 cut(s) 1029
PspN4I GGNNCC 4 cut(s) 318, 689, 1011, 1065
PspPI GGNCC 6 cut(s) 399, 475, 687, 832, 1063, 1198
PspPPI RGGWCCY 1 cut(s) 1063
PsuI RGATCY 1 cut(s) 1009
PsyI GACNNNGTC 1 cut(s) 408
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 5 cut(s) 99, 101, 252, 439, 823
SacI GAGCTC 1 cut(s) 536
SaqAI TTAA 4 cut(s) 131, 459, 765, 977
SatI GCNGC 4 cut(s) 469, 642, 747, 1139
Sau3AI GATC 3 cut(s) 913, 1009, 1192
Sau96I GGNCC 6 cut(s) 399, 475, 687, 832, 1063, 1198
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 2 cut(s) 532, 1015
ScrFI CCNGG 4 cut(s) 424, 835, 903, 1031
SduI GDGCHC 4 cut(s) 43, 536, 817, 949
SfaNI GCATC 2 cut(s) 34, 1238
SinI GGWCC 3 cut(s) 475, 687, 1063
SmiMI CAYNNNNRTG 5 cut(s) 99, 101, 252, 439, 823
SmlI CTYRAG 2 cut(s) 78, 764
SmoI CTYRAG 2 cut(s) 78, 764
SpeI ACTAGT 1 cut(s) 496
Sse9I AATT 2 cut(s) 698, 1130
SsiI CCGC 1 cut(s) 468
SspMI CTAG 2 cut(s) 122, 497
SstI GAGCTC 1 cut(s) 536
StyD4I CCNGG 4 cut(s) 422, 833, 901, 1029
TaaI ACNGT 1 cut(s) 1211
TaqI TCGA 5 cut(s) 90, 167, 297, 530, 1225
TasI AATT 2 cut(s) 698, 1130
TatI WGTACW 1 cut(s) 9
TauI GCSGC 1 cut(s) 471
TfiI GAWTC 4 cut(s) 169, 351, 694, 799
Tru1I TTAA 4 cut(s) 131, 459, 765, 977
Tru9I TTAA 4 cut(s) 131, 459, 765, 977
TscAI CASTG 3 cut(s) 219, 825, 859
TseFI GTSAC 1 cut(s) 1159
TseI GCWGC 3 cut(s) 641, 746, 1138
Tsp45I GTSAC 1 cut(s) 1159
TspDTI ATGAA 7 cut(s) 17, 99, 132, 176, 591, 668, 788
TspGWI ACGGA 3 cut(s) 429, 581, 823
TspRI CASTG 3 cut(s) 219, 825, 859
Tth111I GACNNNGTC 1 cut(s) 408
Vha464I CTTAAG 1 cut(s) 764
VpaK11BI GGWCC 3 cut(s) 475, 687, 1063
XapI RAATTY 1 cut(s) 1130
XceI RCATGY 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 1 cut(s) 1032
XspI CTAG 2 cut(s) 122, 497
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.