Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0000966

Physical Location & Seq

Genomic Coordinates Forward (+)

30507179 .. 30509396

Gene Structure 2218 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1211400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGATGAAAAATAACAAAAATATGAATTCTGGAAAACAGAAATCCCTTGAACTTCCATGTCTCATCAAGTATATATTATCAAATGAAGAGGTTGGCTTTGGTGATGCTGAGGAGATTAGATCCGATGAACGTGAGCATTCTGTTGCTATTAGCATGCCAACATCACCAGAGGAAATCCATTTGCAGAACAAAAAAAGAAATCTCTTCATCGGTGGAATCCCAGCTTCTTCTGCCACACTTAAAGGTGCTAGCAGCAGCACACTGCCGAAGACAGCAAAATTTCACCTTCAGCCAATGGAAAAAAGCTCTGCATTTGAAGAGGAAATGAACACTGGACATTTCCCTTCTCATCCGAGTATTGAAAGTTTGAAAGATAAGAGATATGATACTTTCAAAACATGGTCCAGAAAACTCGACAGGAAGATAACGCTTCTCCATGGAAATAGACCGGGGGAAACTGAATCTGAGAATGTTAATGGGAAAATCGAAGAAATCGAGCGTTTACCTGCAGACCGATACTTTGATGCATTGGAAGGGCCAGAATTGGATACTCTTAGGCCTTCAGAGGAAATACTGCTTCCGGAAGACAAACAGTGGCCATTTCTTCTTCGGTATCCAGTTTCTTCGTTTAGCATCTGCCTCGGCGTTAGCAGCCAAGCAATTTTGTGGAAGGCCCTGCCTACCTCTGCCTCAATGAAATTTCTCCACATGAGCTTAACAATAAATATAGTTTTGTGGTGCATTTCAGTTGTCCTTTTAGCTATTGTTTCTTGCATCTACCTTGTAAAAGTAGCCCTTTACTTTGAAGCAGTTCGCCGTGAGTACCACCATCCAATCCGTATCAATTTCTTCTTTGCTCCATGGATAGCCCTCTTGTTCTTAGCTCTTGGAGTGCCACCTTCATTTGCTAACAACCTGCATCCATCTCTTTGGTACATTCTCATGACTCCGCTTCTATGTCTCGAGCTTAAAATCTATGGACAGTGGATGTTCGGAGGCCAGCGTAGGCTTTCAAAAGTGGCGAATCCTTTCAACCATCTCGCAATAGTTGGGAACTTTGTGGGGGCGTTGCTTGGTGCATCAATGGGACTACAAGAAGGACCAATATTCTTCTTTGCTATAGGGTTTGTTCACTACATTGTATTATTTGTTACTCTCTACCAAAGACTTCCAACAAATGATACCGTGATCCCAAAAGAACTCCATCCTGTGTTCTTTCTCTTTGTTGCAGCACCAAGTATAGCTTCAGTAGCATGGGCAAAAATCCAAGGCTCCTTTGATCATGGCTCGCGGATTGCGTACTTCATTTCCTTGTTCCTTTATCTCTCACTGGTAGTCCGGGTTAATTATTTCCGAGGATTTAAGTTTTCTATGGCATGGTGGGCTTATACTTTCCCAATGACTGGTGCTGCTATTGCAACCATCAAGTATTCAGATGAAGTCACAAATGTGGTAACACAAGCTCTGGCTGTCATACTCTCTCTCATTGCCATAATCACAGTCATTGCTCTTCTCATAACAACTATCTTGCATGGCTTAGTGCTTCAAAACCTCTTCCCTAATGACATTGCAATTGCCATTAGTGACAGAAAGCCAAACCCTAACAAGAAGTGGTTCCACGTAAATGGCTACGAGAACAACGATTCAGAAGCTGTCAAGATCGTGACCCATTTTGAGATGCAACCCCAAACTTCAGAGAGAAACTTGCATCGAGATGCCATGGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005253 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006821 GO:0006873 GO:0006950 GO:0006970 GO:0008150 GO:0008308 GO:0008509 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009987 GO:0010035 GO:0015075 GO:0015103 GO:0015267 GO:0015318 GO:0015698 GO:0016020 GO:0019725 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022857 GO:0034220 GO:0042221 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0071944 GO:0098656 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SLAC1

Protein Analysis

576

Amino Acids

65.0

Weight (kDa)

8.38

Isoelectric Point (pI)

41.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SLAC1	PF03595	205 - 509	1e-45	Voltage-dependent anion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000966)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27970 AT4G27970 AT4G27970 AT4G27970 AT4G27970 AT4G27970 AT5G24030 AT5G24030
fragaria_vesca	FvH4_7g30630 FvH4_7g30630 FvH4_7g30630 FvH4_7g30640 FvH4_7g30640 FvH4_7g30640 FvH4_7g30640
malus_domestica	MD01G1211400.v1.1 MD01G1211500.v1.1 MD07G1282100.v1.1
prunus_persica	Prupe.2G291700_v2.0.a1 Prupe.2G304200_v2.0.a1 Prupe.2G304300_v2.0.a1 Prupe.2G304300_v2.0.a1 Prupe.2G304300_v2.0.a1
pyrus_communis	pycom01g22190 pycom01g22200 pycom07g25600
rosa_chinensis	RchiOBHm_Chr1g0369441 RchiOBHm_Chr1g0379541 RchiOBHm_Chr1g0379571
rosa_laevigata	RLG00000026393 RLG00000026394 RLG00000027130
rosa_multiflora	Rmu_co8329679.1_g000001 Rmu_sc0000402.1_g000002 Rmu_sc0000402.1_g000005 Rmu_sc0001985.1_g000002 Rmu_sc0003612.1_g000018 Rmu_sc0024534.1_g000001
rosa_roxburghii	Rroxscaffold_4G00280080 Rroxscaffold_4G00280090 Rroxscaffold_4G00287650
rosa_rugosa	Rorug01G0350200 Rorug01G0350200 Rorug01G0413000
rosa_samantha	Rh1AG357900 Rh1AG435500 Rh1AG435600 Rh1BG322000 Rh1BG393100 Rh1BG393200 Rh1CG335700 Rh1CG405800 Rh1CG405900 Rh1DG352600 Rh1DG423900 Rh1DG424000
rosa_wichuraiana	Rw0G019950 Rw1G031570 Rw1G031580 Rw1G038040 Rw1G038050 Rw1G038070 Rw1G038080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	514, 924
AccB7I	CCANNNNNTGG	1 cut(s)	1403
AccII	CGCG	1 cut(s)	1291
AccIII	TCCGGA	1 cut(s)	580
AciI	CCGC	2 cut(s)	950, 1291
AclWI	GGATC	2 cut(s)	114, 1183
AcoI	YGGCCR	1 cut(s)	596
AcsI	RAATTY	3 cut(s)	25, 278, 698
AcuI	CTGAAG	4 cut(s)	272, 546, 1230, 1677
AfaI	GTAC	3 cut(s)	824, 935, 1301
AfiI	CCNNNNNNNGG	1 cut(s)	1403
AgsI	TTSAA	9 cut(s)	50, 317, 362, 370, 394, 806, 1014, 1033, 1547
AjuI	GAANNNNNNNTTGG	2 cut(s)	1228, 1260
AleI	CACNNNNGTG	1 cut(s)	1448
AluBI	AGCT	9 cut(s)	224, 306, 714, 761, 884, 967, 1244, 1463, 1652
AluI	AGCT	9 cut(s)	224, 306, 714, 761, 884, 967, 1244, 1463, 1652
Alw26I	GTCTC	2 cut(s)	65, 965
AlwI	GGATC	2 cut(s)	114, 1183
AlwNI	CAGNNNCTG	1 cut(s)	1652
Ama87I	CYCGRG	1 cut(s)	962
Aor13HI	TCCGGA	1 cut(s)	580
AoxI	GGCC	5 cut(s)	536, 557, 596, 672, 997
ApeKI	GCWGC	5 cut(s)	252, 255, 651, 1229, 1409
ApoI	RAATTY	3 cut(s)	25, 278, 698
ArsI	GACNNNNNNTTYG	2 cut(s)	503, 535
Asp700I	GAANNNNTTC	3 cut(s)	810, 1028, 1613
AspS9I	GGNCC	4 cut(s)	402, 536, 673, 1100
AsuC2I	CCSGG	2 cut(s)	450, 1340
AsuHPI	GGTGA	3 cut(s)	113, 156, 275
AsuNHI	GCTAGC	1 cut(s)	248
AvaI	CYCGRG	1 cut(s)	962
AvaII	GGWCC	2 cut(s)	402, 1100
BalI	TGGCCA	1 cut(s)	598
BbsI	GAAGAC	2 cut(s)	275, 591
BbvCI	CCTCAGC	1 cut(s)	108
BbvI	GCAGC	5 cut(s)	264, 267, 663, 1241, 1396
BccI	CCATC	5 cut(s)	837, 931, 1044, 1212, 1430
BceAI	ACGGC	1 cut(s)	801
BcgI	CGANNNNNNTGC	2 cut(s)	622, 656
BciVI	GTATCC	2 cut(s)	541, 624
BclI	TGATCA	1 cut(s)	1279
BcnI	CCSGG	2 cut(s)	450, 1340
BcoDI	GTCTC	2 cut(s)	65, 965
BfaI	CTAG	1 cut(s)	249
BfmI	CTRYAG	2 cut(s)	507, 1119
BfuAI	ACCTGC	2 cut(s)	514, 924
BfuI	GTATCC	2 cut(s)	541, 624
BisI	GCNGC	5 cut(s)	253, 256, 652, 1230, 1410
BlsI	GCNGC	5 cut(s)	254, 257, 653, 1231, 1411
Bme1390I	CCNGG	2 cut(s)	450, 1340
Bme18I	GGWCC	2 cut(s)	402, 1100
BmeT110I	CYCGRG	1 cut(s)	962
BmgT120I	GGNCC	4 cut(s)	402, 536, 673, 1100
BmiI	GGNNCC	2 cut(s)	1273, 1616
BmrFI	CCNGG	2 cut(s)	450, 1340
BmsI	GCATC	9 cut(s)	94, 514, 642, 783, 928, 1088, 1668, 1705, 1717
BmtI	GCTAGC	1 cut(s)	252
BpiI	GAAGAC	2 cut(s)	275, 591
Bpu10I	CCTNAGC	1 cut(s)	108
BpuMI	CCSGG	2 cut(s)	450, 1340
BsaAI	YACGTR	1 cut(s)	1621
BsaJI	CCNNGG	7 cut(s)	436, 449, 640, 860, 1267, 1354, 1719
BsaWI	WCCGGW	1 cut(s)	580
Bsc4I	CCNNNNNNNGG	1 cut(s)	1403
Bse1I	ACTGG	4 cut(s)	337, 617, 1335, 1408
Bse3DI	GCAATG	3 cut(s)	1485, 1503, 1566
BseAI	TCCGGA	1 cut(s)	580
BseDI	CCNNGG	7 cut(s)	436, 449, 640, 860, 1267, 1354, 1719
BseGI	GGATG	5 cut(s)	349, 829, 919, 993, 1204
BseLI	CCNNNNNNNGG	1 cut(s)	1403
BseMI	GCAATG	3 cut(s)	1485, 1503, 1566
BseMII	CTCAG	2 cut(s)	99, 456
BseNI	ACTGG	4 cut(s)	337, 617, 1335, 1408
BseRI	GAGGAG	1 cut(s)	125
BseXI	GCAGC	5 cut(s)	264, 267, 663, 1241, 1396
BseYI	CCCAGC	1 cut(s)	220
Bsh1236I	CGCG	1 cut(s)	1291
BshFI	GGCC	5 cut(s)	538, 559, 598, 674, 999
BsiHKCI	CYCGRG	1 cut(s)	962
BsiSI	CCGG	3 cut(s)	449, 581, 1339
BslFI	GGGAC	1 cut(s)	1101
BslI	CCNNNNNNNGG	1 cut(s)	1403
BsmAI	GTCTC	2 cut(s)	65, 965
BsmFI	GGGAC	1 cut(s)	1101
BsmI	GAATGC	1 cut(s)	136
BsnI	GGCC	5 cut(s)	538, 559, 598, 674, 999
BsoBI	CYCGRG	1 cut(s)	962
Bsp13I	TCCGGA	1 cut(s)	580
Bsp143I	GATC	4 cut(s)	119, 1188, 1279, 1659
Bsp19I	CCATGG	3 cut(s)	436, 860, 1719
BspACI	CCGC	2 cut(s)	950, 1291
BspANI	GGCC	5 cut(s)	538, 559, 598, 674, 999
BspCNI	CTCAG	2 cut(s)	100, 457
BspEI	TCCGGA	1 cut(s)	580
BspFNI	CGCG	1 cut(s)	1291
BspHI	TCATGA	1 cut(s)	942
BspLI	GGNNCC	2 cut(s)	1273, 1616
BspMAI	CTGCAG	1 cut(s)	511
BspMI	ACCTGC	2 cut(s)	514, 924
BspOI	GCTAGC	1 cut(s)	252
BspPI	GGATC	2 cut(s)	114, 1183
BspQI	GCTCTTC	1 cut(s)	1515
BsrDI	GCAATG	3 cut(s)	1485, 1503, 1566
BsrI	ACTGG	4 cut(s)	337, 617, 1335, 1408
BssECI	CCNNGG	7 cut(s)	436, 449, 640, 860, 1267, 1354, 1719
BssMI	GATC	4 cut(s)	119, 1188, 1279, 1659
BssT1I	CCWWGG	4 cut(s)	436, 860, 1267, 1719
Bst4CI	ACNGT	4 cut(s)	594, 984, 1186, 1501
Bst6I	CTCTTC	5 cut(s)	81, 209, 312, 1515, 1559
BstBAI	YACGTR	1 cut(s)	1621
BstC8I	GCNNGC	4 cut(s)	155, 250, 1001, 1289
BstDEI	CTNAG	5 cut(s)	108, 465, 554, 880, 1537
BstDSI	CCRYGG	3 cut(s)	436, 860, 1719
BstF5I	GGATG	5 cut(s)	349, 829, 919, 993, 1204
BstFNI	CGCG	1 cut(s)	1291
BstKTI	GATC	4 cut(s)	122, 1191, 1282, 1662
BstMAI	GTCTC	2 cut(s)	65, 965
BstMBI	GATC	4 cut(s)	119, 1188, 1279, 1659
BstMWI	GCNNNNNNNGC	5 cut(s)	230, 651, 1250, 1382, 1415
BstNSI	RCATGY	1 cut(s)	157
BstSCI	CCNGG	2 cut(s)	448, 1338
BstSFI	CTRYAG	2 cut(s)	507, 1119
BstUI	CGCG	1 cut(s)	1291
BstV1I	GCAGC	5 cut(s)	264, 267, 663, 1241, 1396
BstV2I	GAAGAC	2 cut(s)	275, 591
BstX2I	RGATCY	1 cut(s)	119
BstXI	CCANNNNNNTGG	2 cut(s)	930, 1625
BstYI	RGATCY	1 cut(s)	119
BsuI	GTATCC	2 cut(s)	541, 624
BsuRI	GGCC	5 cut(s)	538, 559, 598, 674, 999
BtgI	CCRYGG	3 cut(s)	436, 860, 1719
BtsCI	GGATG	5 cut(s)	349, 829, 919, 993, 1204
BtsI	GCAGTG	1 cut(s)	260
BtsIMutI	CAGTG	5 cut(s)	260, 330, 599, 989, 1328
BveI	ACCTGC	2 cut(s)	514, 924
Cac8I	GCNNGC	4 cut(s)	155, 250, 1001, 1289
CaiI	CAGNNNCTG	1 cut(s)	1652
CciI	TCATGA	1 cut(s)	942
Cfr13I	GGNCC	4 cut(s)	402, 536, 673, 1100
Csp6I	GTAC	3 cut(s)	823, 934, 1300
CviQI	GTAC	3 cut(s)	823, 934, 1300
DdeI	CTNAG	5 cut(s)	108, 465, 554, 880, 1537
DpnI	GATC	4 cut(s)	121, 1190, 1281, 1661
DpnII	GATC	4 cut(s)	119, 1188, 1279, 1659
EaeI	YGGCCR	1 cut(s)	596
Eam1104I	CTCTTC	5 cut(s)	81, 209, 312, 1515, 1559
EarI	CTCTTC	5 cut(s)	81, 209, 312, 1515, 1559
Eco130I	CCWWGG	4 cut(s)	436, 860, 1267, 1719
Eco147I	AGGCCT	1 cut(s)	559
Eco47I	GGWCC	2 cut(s)	402, 1100
Eco57I	CTGAAG	4 cut(s)	272, 546, 1230, 1677
Eco88I	CYCGRG	1 cut(s)	962
EcoO109I	RGGNCCY	1 cut(s)	673
EcoRI	GAATTC	1 cut(s)	25
EcoT14I	CCWWGG	4 cut(s)	436, 860, 1267, 1719
EcoT22I	ATGCAT	1 cut(s)	529
ErhI	CCWWGG	4 cut(s)	436, 860, 1267, 1719
FalI	AAGNNNNNCTT	4 cut(s)	780, 812, 1228, 1260
FaqI	GGGAC	1 cut(s)	1101
FbaI	TGATCA	1 cut(s)	1279
Fnu4HI	GCNGC	5 cut(s)	253, 256, 652, 1230, 1410
FokI	GGATG	5 cut(s)	336, 816, 906, 1000, 1191
Fsp4HI	GCNGC	5 cut(s)	253, 256, 652, 1230, 1410
FspBI	CTAG	1 cut(s)	249
GluI	GCNGC	5 cut(s)	253, 256, 652, 1230, 1410
GsaI	CCCAGC	1 cut(s)	224
HaeIII	GGCC	5 cut(s)	538, 559, 598, 674, 999
HapII	CCGG	3 cut(s)	449, 581, 1339
HinfI	GANTC	5 cut(s)	216, 461, 946, 1024, 1643
HpaII	CCGG	3 cut(s)	449, 581, 1339
HphI	GGTGA	3 cut(s)	113, 156, 275
Hpy166II	GTNNAC	2 cut(s)	503, 1132
Hpy188I	TCNGA	9 cut(s)	124, 354, 466, 565, 995, 1355, 1435, 1648, 1696
Hpy188III	TCNNGA	8 cut(s)	30, 405, 581, 943, 962, 1657, 1663, 1712
Hpy8I	GTNNAC	2 cut(s)	503, 1132
HpyAV	CCTTC	7 cut(s)	296, 354, 527, 570, 664, 909, 1091
HpyCH4III	ACNGT	4 cut(s)	594, 984, 1186, 1501
HpyCH4IV	ACGT	2 cut(s)	130, 1620
HpyF10VI	GCNNNNNNNGC	5 cut(s)	230, 651, 1250, 1382, 1415
HpyF3I	CTNAG	5 cut(s)	108, 465, 554, 880, 1537
HpySE526I	ACGT	2 cut(s)	130, 1620
Kpn2I	TCCGGA	1 cut(s)	580
Ksp22I	TGATCA	1 cut(s)	1279
Kzo9I	GATC	4 cut(s)	119, 1188, 1279, 1659
LguI	GCTCTTC	1 cut(s)	1515
LmnI	GCTCC	2 cut(s)	862, 1277
Lsp1109I	GCAGC	5 cut(s)	264, 267, 663, 1241, 1396
LweI	GCATC	9 cut(s)	94, 514, 642, 783, 928, 1088, 1668, 1705, 1717
MaeI	CTAG	1 cut(s)	249
MaeII	ACGT	2 cut(s)	130, 1620
MaeIII	GTNAC	5 cut(s)	1150, 1441, 1453, 1583, 1663
MalI	GATC	4 cut(s)	121, 1190, 1281, 1661
MboI	GATC	4 cut(s)	119, 1188, 1279, 1659
MfeI	CAATTG	1 cut(s)	1572
MflI	RGATCY	1 cut(s)	119
MlsI	TGGCCA	1 cut(s)	598
MluCI	AATT	8 cut(s)	25, 278, 542, 660, 698, 844, 1345, 1572
MluNI	TGGCCA	1 cut(s)	598
MlyI	GAGTC	1 cut(s)	940
MmeI	TCCRAC	1 cut(s)	1196
Mox20I	TGGCCA	1 cut(s)	598
Mph1103I	ATGCAT	1 cut(s)	529
MroI	TCCGGA	1 cut(s)	580
MroXI	GAANNNNTTC	3 cut(s)	810, 1028, 1613
MscI	TGGCCA	1 cut(s)	598
MseI	TTAA	6 cut(s)	240, 474, 716, 969, 1344, 1362
MslI	CAYNNNNRTG	4 cut(s)	941, 1448, 1623, 1713
Msp20I	TGGCCA	1 cut(s)	598
MspI	CCGG	3 cut(s)	449, 581, 1339
MspR9I	CCNGG	2 cut(s)	450, 1340
MunI	CAATTG	1 cut(s)	1572
Mva1269I	GAATGC	1 cut(s)	136
MvnI	CGCG	1 cut(s)	1291
MwoI	GCNNNNNNNGC	5 cut(s)	230, 651, 1250, 1382, 1415
NciI	CCSGG	2 cut(s)	450, 1340
NcoI	CCATGG	3 cut(s)	436, 860, 1719
NdeII	GATC	4 cut(s)	119, 1188, 1279, 1659
NheI	GCTAGC	1 cut(s)	248
NlaIV	GGNNCC	2 cut(s)	1273, 1616
NmeAIII	GCCGAG	1 cut(s)	621
NmuCI	GTSAC	3 cut(s)	1441, 1583, 1663
NsiI	ATGCAT	1 cut(s)	529
NspI	RCATGY	1 cut(s)	157
OliI	CACNNNNGTG	1 cut(s)	1448
PaeI	GCATGC	1 cut(s)	157
PaeR7I	CTCGAG	1 cut(s)	962
PagI	TCATGA	1 cut(s)	942
PceI	AGGCCT	1 cut(s)	559
PciSI	GCTCTTC	1 cut(s)	1515
PcsI	WCGNNNNNNNCGW	3 cut(s)	492, 1295, 1638
PctI	GAATGC	1 cut(s)	136
PdmI	GAANNNNTTC	3 cut(s)	810, 1028, 1613
PfeI	GAWTC	4 cut(s)	216, 461, 1024, 1643
PflMI	CCANNNNNTGG	1 cut(s)	1403
PkrI	GCNGC	5 cut(s)	254, 257, 653, 1231, 1411
PleI	GAGTC	1 cut(s)	940
PpsI	GAGTC	1 cut(s)	940
Ppu21I	YACGTR	1 cut(s)	1621
PspFI	CCCAGC	1 cut(s)	220
PspN4I	GGNNCC	2 cut(s)	1273, 1616
PspPI	GGNCC	4 cut(s)	402, 536, 673, 1100
PstI	CTGCAG	1 cut(s)	511
PstNI	CAGNNNCTG	1 cut(s)	1652
PsuI	RGATCY	1 cut(s)	119
RsaI	GTAC	3 cut(s)	824, 935, 1301
RsaNI	GTAC	3 cut(s)	823, 934, 1300
RseI	CAYNNNNRTG	4 cut(s)	941, 1448, 1623, 1713
SapI	GCTCTTC	1 cut(s)	1515
SaqAI	TTAA	6 cut(s)	240, 474, 716, 969, 1344, 1362
SatI	GCNGC	5 cut(s)	253, 256, 652, 1230, 1410
Sau3AI	GATC	4 cut(s)	119, 1188, 1279, 1659
Sau96I	GGNCC	4 cut(s)	402, 536, 673, 1100
SchI	GAGTC	1 cut(s)	940
ScrFI	CCNGG	2 cut(s)	450, 1340
SfaNI	GCATC	9 cut(s)	94, 514, 642, 783, 928, 1088, 1668, 1705, 1717
SfcI	CTRYAG	2 cut(s)	507, 1119
Sfr274I	CTCGAG	1 cut(s)	962
SinI	GGWCC	2 cut(s)	402, 1100
SlaI	CTCGAG	1 cut(s)	962
SmiMI	CAYNNNNRTG	4 cut(s)	941, 1448, 1623, 1713
SmlI	CTYRAG	1 cut(s)	962
SmoI	CTYRAG	1 cut(s)	962
SphI	GCATGC	1 cut(s)	157
Sse9I	AATT	8 cut(s)	25, 278, 542, 660, 698, 844, 1345, 1572
SseBI	AGGCCT	1 cut(s)	559
SsiI	CCGC	2 cut(s)	950, 1291
SspI	AATATT	1 cut(s)	1107
SspMI	CTAG	1 cut(s)	249
StuI	AGGCCT	1 cut(s)	559
StyD4I	CCNGG	2 cut(s)	448, 1338
StyI	CCWWGG	4 cut(s)	436, 860, 1267, 1719
TaaI	ACNGT	4 cut(s)	594, 984, 1186, 1501
TaiI	ACGT	2 cut(s)	133, 1623
TaqI	TCGA	5 cut(s)	414, 486, 495, 963, 1711
TaqII	GACCGA	1 cut(s)	528
TasI	AATT	8 cut(s)	25, 278, 542, 660, 698, 844, 1345, 1572
TfiI	GAWTC	4 cut(s)	216, 461, 1024, 1643
Tru1I	TTAA	6 cut(s)	240, 474, 716, 969, 1344, 1362
Tru9I	TTAA	6 cut(s)	240, 474, 716, 969, 1344, 1362
TscAI	CASTG	5 cut(s)	267, 337, 599, 989, 1335
TseFI	GTSAC	3 cut(s)	1441, 1583, 1663
TseI	GCWGC	5 cut(s)	252, 255, 651, 1229, 1409
Tsp45I	GTSAC	3 cut(s)	1441, 1583, 1663
TspGWI	ACGGA	1 cut(s)	827
TspRI	CASTG	5 cut(s)	267, 337, 599, 989, 1335
Van91I	CCANNNNNTGG	1 cut(s)	1403
VpaK11BI	GGWCC	2 cut(s)	402, 1100
XapI	RAATTY	3 cut(s)	25, 278, 698
XceI	RCATGY	1 cut(s)	157
XhoI	CTCGAG	1 cut(s)	962
XmnI	GAANNNNTTC	3 cut(s)	810, 1028, 1613
XspI	CTAG	1 cut(s)	249
Zsp2I	ATGCAT	1 cut(s)	529

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.