Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002889

Physical Location & Seq

Genomic Coordinates Forward (+)

6232948 .. 6238819

Gene Structure 5872 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1079200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGGGTGTTGGAATTCAAACAATGTGGAGCATTCTGCTACTGCTTTCCTGCATTTTTTCTGCAGCTTCAGCTTCCGTGGGTTATGACCACAAAGCTATAATAATTAATGGGCAGAGAAGGATTTTAATTTCTGGATCAATTCACTATCCCAGAAGCACTCCTGAGATGTGGCCGGATTTGATTCAGAAGGCCAAAGCTGGAGGCTTGGATGTTATACAGACCTATGTGTTTTGGAATGGCCATGAACCTTCTCCAGGAAAATATTATTTTGAGGACAGATATGATTTGGTCAAGTTCATCAAGCTGGTGCAACAGGCAGGCCTCTTTGTTAATCTCCGGATTGGGCCATATGTCTGTGCTGAATGGAACTTCGGGGGATTCCCAATTTGGCTGAAATATGTTCCCGGAATCGCTTTTCGAACAGACAATGAGCCTTTCAAGGCGGCAATGCAAAAATTTACAGAGAAGATTGTCAACATGATGAAGGCAGAGAAGCTGTTCCAAACTGAAGGAGGTCCTATAATTCTTTCTCAGATAGAAAATGAATATGGACCTGTGGAATGGGAAATTGGTGCACCAGGAAAAGCTTACACCAAATGGGCAGCTCAGATGGCTGTAGGTCTCAACACTGGAGTCCCATGGATTATGTGCAAGCAAGAGGATGCCCCCGATCCCGTTATTGACACCTGCAATGGTTACTACTGTGAGAATTTCAAGCCAAACAAGGTCTACAAGCCAAAAATGTGGACAGAAGTCTGGACTGGTTGGTATACAGAATTCGGTGGGGCAATTCCCACTAGACCTGTCGAAGATTTGGCATTTTCAGTTGCTAGGTTCATACAAAGTGGCGGTTCTTTTTTCAACTATTACATGTACCACGGAGGAACGAATTTTGGCAGAACTGCTGGAGGTCCCTTCATGGCCACCAGCTATGACTATGACGCCCCCTTAGACGAATATGGACTACTCCAGCAACCAAAGTGGGGACATTTGAAAGATCTGCACAAAGCCATCAAGTCATGCGAGTATGCTTTGGTGGCCGTTGATCCTTCAGTGACTAAACTTGGAAATAATCAAGAGGCTCATGTGTTCAATACAAAGTCGGGGTGCGCTGCATTCCTCGCAAACTATGACACGAAATACCCTGTTAGAGTGAGCTTTGGACAGGGGCAGTATGACCTGCCACCATGGTCCATCAGCATTCTCCCGGACTGCAAAACTGCAGTTTTCAACACTGCAAAGGTCACTTGGAAAACCTCGCAAGTGCAGATGAAACCAGTATATGGTAGACTTCCTTGGCAGTCATTCATCGAAGAAACCACCACTTCTGATGAGTCCGGTACAACTACGTTAGATGGGTTGTATGAACAGATATATATGACTAGGGATGCTACTGACTACTTGTGGTACATGACAGACATCACAATAGGTTCTGATGAAGCGTTTCTAAACAACGGAAAGTTCCCACTTCTTACGATCTTTTCAGCAGGTCATGCCTTACATGTTTTCATCAATGGTCAGCTATCAGGAACCGTGTATGGGTCATTGGAGAATCCTAAACTAACATTCAGTCAGAACGTGAAGTTGAGACCCGGCATCAACAAGCTTGCATTGCTTAGCATTTCTGTTGGTCTGCCGAATGTTGGAACTCACTTTGAAACATGGAACGCGGGAGTTCTAGGCCCGATCTCATTGAAGGGTTTGAATACGGGAACATGGGACATGTCACGATGGAAATGGACCTACAAGATTGGTATGAAAGGTGAAGCTTTAGGCCTGCATACAGTTACTGGGAGTTCTTCTGTTGACTGGGCAGAAGGACCGTCGATGGCTAAAAAACAACCCCTTACGTGGTACAAGGCAACTTTCAATGCACCACCAGGTCATGCCCCATTAGCTTTAGATATGGGAAGCATGGGAAAAGGTCAAATATGGATAAACGGACAGAGCGTTGGACGCCATTGGCCTGGATACATTGCGCAGGGTAGCTGTGGAACATGTAATTATGCCGGAACTTTTTATGATAAGAAATGCAGAACTTATTGCGGCAAGCCTTCCCAGAGATGGTACCATATTCCTCGATCGTGGTTGACCCCGACTGGAAATCTACTGGTGGTGTTCGAAGAATGGGGTGGTGATCCGCAATGGATGTCTTTAGTCGAAAGAGTAAGAGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

726

Amino Acids

81.08

Weight (kDa)

8.23

Isoelectric Point (pI)

35.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	33 - 337	6.3e-120	Glycosyl hydrolases family 35
GHD	PF17834	360 - 415	4.9e-21	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	467 - 534	1.4e-07	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	616 - 702	1.1e-24	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56870
fragaria_vesca	FvH4_1g07401 FvH4_1g07410 FvH4_1g07410
malus_domestica	MD02G1079200.v1.1 MD08G1139000.v1.1 MD13G1126600.v1.1 MD15G1206800.v1.1
prunus_persica	Prupe.7G210000_v2.0.a1 Prupe.7G210000_v2.0.a1
pyrus_communis	pycom02g06300 pycom15g18320
rosa_chinensis	RchiOBHm_Chr2g0093301 RchiOBHm_Chr2g0093311 RchiOBHm_Chr3g0459401 RchiOBHm_Chr3g0459561
rosa_laevigata	RLG00000016388 RLG00000016389 RLG00000025083
rosa_multiflora	Rmu_sc0002089.1_g000005
rosa_roxburghii	Rroxscaffold_2G00148200 Rroxscaffold_2G00148210 Rroxscaffold_5G00345960
rosa_rugosa	Rorug02G0034700.1 Rorug03G0036300
rosa_samantha	Rh2AG080700 Rh2AG080800 Rh2CG084000 Rh2DG079700 Rh3AG094200 Rh3BG097100 Rh3CG098400 Rh3DG097900 Rh3DG098900
rosa_wichuraiana	Rw2G006600 Rw2G006610 Rw3G008110 Rw3G008200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	695
Acc16I	TGCGCA	1 cut(s)	1980
Acc36I	ACCTGC	3 cut(s)	695, 1188, 1478
Acc65I	GGTACC	1 cut(s)	2067
AccB1I	GGYRCC	1 cut(s)	2067
AccB7I	CCANNNNNTGG	1 cut(s)	1283
AccI	GTMKAC	3 cut(s)	729, 770, 1288
AccII	CGCG	1 cut(s)	1670
AccIII	TCCGGA	1 cut(s)	336
AciI	CCGC	5 cut(s)	443, 849, 1670, 2046, 2141
AclWI	GGATC	4 cut(s)	142, 665, 1040, 2132
AcoI	YGGCCR	4 cut(s)	170, 238, 921, 1038
AcsI	RAATTY	5 cut(s)	12, 455, 709, 776, 889
AcuI	CTGAAG	3 cut(s)	51, 528, 1035
AcyI	GRCGYC	2 cut(s)	942, 1957
AfaI	GTAC	5 cut(s)	875, 1342, 1409, 1856, 2069
AfiI	CCNNNNNNNGG	7 cut(s)	254, 983, 1283, 1539, 1643, 1851, 2064
AflIII	ACRYGT	4 cut(s)	870, 1501, 1722, 1997
AjnI	CCWGG	4 cut(s)	253, 577, 1879, 1966
AjuI	GAANNNNNNNTTGG	2 cut(s)	552, 584
Alw21I	GWGCWC	1 cut(s)	577
Alw26I	GTCTC	2 cut(s)	626, 1582
Alw44I	GTGCAC	1 cut(s)	573
AlwI	GGATC	4 cut(s)	142, 665, 1040, 2132
AlwNI	CAGNNNCTG	1 cut(s)	1790
Aor13HI	TCCGGA	1 cut(s)	336
ApaLI	GTGCAC	1 cut(s)	573
ApeKI	GCWGC	3 cut(s)	62, 602, 1112
ApoI	RAATTY	5 cut(s)	12, 455, 709, 776, 889
AseI	ATTAAT	1 cut(s)	105
Asp700I	GAANNNNTTC	2 cut(s)	497, 1443
Asp718I	GGTACC	1 cut(s)	2067
AspLEI	GCGC	2 cut(s)	1112, 1981
AspS9I	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1740, 1820
AsuC2I	CCSGG	3 cut(s)	405, 1208, 1593
AsuHPI	GGTGA	2 cut(s)	1775, 2147
AsuII	TTCGAA	2 cut(s)	418, 2121
AvaII	GGWCC	6 cut(s)	515, 551, 911, 1191, 1740, 1820
BaeGI	GKGCMC	1 cut(s)	577
BalI	TGGCCA	2 cut(s)	240, 923
BanI	GGYRCC	1 cut(s)	2067
Bbv12I	GWGCWC	1 cut(s)	577
BbvI	GCAGC	3 cut(s)	74, 614, 1099
BccI	CCATC	7 cut(s)	604, 1019, 1202, 1349, 1725, 1822, 2058
BceAI	ACGGC	1 cut(s)	1025
BciT130I	CCWGG	4 cut(s)	255, 579, 1881, 1968
BciVI	GTATCC	1 cut(s)	1964
BcnI	CCSGG	3 cut(s)	405, 1208, 1593
BcoDI	GTCTC	2 cut(s)	626, 1582
BfaI	CTAG	4 cut(s)	798, 831, 1383, 1679
BfmI	CTRYAG	3 cut(s)	60, 615, 1221
BfuAI	ACCTGC	3 cut(s)	695, 1188, 1478
BfuI	GTATCC	1 cut(s)	1964
BglII	AGATCT	1 cut(s)	997
BisI	GCNGC	5 cut(s)	63, 444, 603, 1113, 2047
BlpI	GCTNAGC	1 cut(s)	1616
BlsI	GCNGC	5 cut(s)	64, 445, 604, 1114, 2048
Bme1390I	CCNGG	7 cut(s)	255, 405, 579, 1208, 1593, 1881, 1968
Bme18I	GGWCC	6 cut(s)	515, 551, 911, 1191, 1740, 1820
BmgT120I	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1740, 1820
BmiI	GGNNCC	3 cut(s)	913, 1531, 2069
BmrFI	CCNGG	7 cut(s)	255, 405, 579, 1208, 1593, 1881, 1968
BmrI	ACTGGG	2 cut(s)	1800, 1819
BmsI	GCATC	3 cut(s)	652, 1378, 1605
BmuI	ACTGGG	2 cut(s)	1800, 1819
BoxI	GACNNNNGTC	1 cut(s)	752
BpmI	CTGGAG	5 cut(s)	219, 237, 651, 927, 953
Bpu1102I	GCTNAGC	1 cut(s)	1616
Bpu14I	TTCGAA	2 cut(s)	418, 2121
BpuMI	CCSGG	3 cut(s)	405, 1208, 1593
BsaAI	YACGTR	1 cut(s)	1851
BsaHI	GRCGYC	2 cut(s)	942, 1957
BsaI	GGTCTC	2 cut(s)	626, 1582
BsaJI	CCNNGG	5 cut(s)	75, 638, 877, 1187, 1295
BsaWI	WCCGGW	2 cut(s)	336, 1337
Bsc4I	CCNNNNNNNGG	7 cut(s)	254, 983, 1283, 1539, 1643, 1851, 2064
Bse1I	ACTGG	7 cut(s)	634, 766, 1277, 1795, 1814, 2104, 2115
Bse3DI	GCAATG	5 cut(s)	453, 697, 1610, 1974, 2150
BseAI	TCCGGA	1 cut(s)	336
BseBI	CCWGG	4 cut(s)	255, 579, 1881, 1968
BseDI	CCNNGG	5 cut(s)	75, 638, 877, 1187, 1295
BseGI	GGATG	4 cut(s)	214, 667, 1393, 2154
BseLI	CCNNNNNNNGG	7 cut(s)	254, 983, 1283, 1539, 1643, 1851, 2064
BseMI	GCAATG	5 cut(s)	453, 697, 1610, 1974, 2150
BseMII	CTCAG	3 cut(s)	153, 545, 620
BseNI	ACTGG	7 cut(s)	634, 766, 1277, 1795, 1814, 2104, 2115
BseSI	GKGCMC	1 cut(s)	577
BseXI	GCAGC	3 cut(s)	74, 614, 1099
BsgI	GTGCAG	2 cut(s)	986, 1286
Bsh1236I	CGCG	1 cut(s)	1670
Bsh1285I	CGRYCG	1 cut(s)	2084
BshNI	GGYRCC	1 cut(s)	2067
BsiEI	CGRYCG	1 cut(s)	2084
BsiHKAI	GWGCWC	1 cut(s)	577
BsiSI	CCGG	7 cut(s)	173, 337, 405, 1208, 1338, 1593, 2010
BslFI	GGGAC	4 cut(s)	620, 897, 999, 1733
BslI	CCNNNNNNNGG	7 cut(s)	254, 983, 1283, 1539, 1643, 1851, 2064
BsmAI	GTCTC	2 cut(s)	626, 1582
BsmFI	GGGAC	4 cut(s)	620, 897, 999, 1733
BsmI	GAATGC	3 cut(s)	30, 1115, 1200
Bso31I	GGTCTC	2 cut(s)	626, 1582
Bsp119I	TTCGAA	2 cut(s)	418, 2121
Bsp1286I	GDGCHC	1 cut(s)	577
Bsp13I	TCCGGA	1 cut(s)	336
Bsp143I	GATC	8 cut(s)	134, 670, 997, 1045, 1476, 1686, 2081, 2137
Bsp1720I	GCTNAGC	1 cut(s)	1616
Bsp19I	CCATGG	2 cut(s)	638, 1187
BspACI	CCGC	5 cut(s)	443, 849, 1670, 2046, 2141
BspCNI	CTCAG	3 cut(s)	154, 544, 619
BspEI	TCCGGA	1 cut(s)	336
BspFNI	CGCG	1 cut(s)	1670
BspLI	GGNNCC	3 cut(s)	913, 1531, 2069
BspMAI	CTGCAG	2 cut(s)	64, 1225
BspMI	ACCTGC	3 cut(s)	695, 1188, 1478
BspPI	GGATC	4 cut(s)	142, 665, 1040, 2132
BspT104I	TTCGAA	2 cut(s)	418, 2121
BspT107I	GGYRCC	1 cut(s)	2067
BspTNI	GGTCTC	2 cut(s)	626, 1582
BsrDI	GCAATG	5 cut(s)	453, 697, 1610, 1974, 2150
BsrI	ACTGG	7 cut(s)	634, 766, 1277, 1795, 1814, 2104, 2115
BssECI	CCNNGG	5 cut(s)	75, 638, 877, 1187, 1295
BssMI	GATC	8 cut(s)	134, 670, 997, 1045, 1476, 1686, 2081, 2137
BssNAI	GTATAC	1 cut(s)	771
BssNI	GRCGYC	2 cut(s)	942, 1957
BssT1I	CCWWGG	3 cut(s)	638, 1187, 1295
Bst1107I	GTATAC	1 cut(s)	771
Bst2UI	CCWGG	4 cut(s)	255, 579, 1881, 1968
Bst4CI	ACNGT	4 cut(s)	704, 1534, 1786, 1824
BstACI	GRCGYC	2 cut(s)	942, 1957
BstAPI	GCANNNNNTGC	1 cut(s)	1493
BstBAI	YACGTR	1 cut(s)	1851
BstBI	TTCGAA	2 cut(s)	418, 2121
BstC8I	GCNNGC	5 cut(s)	319, 653, 1608, 1778, 2051
BstDEI	CTNAG	5 cut(s)	162, 531, 606, 949, 1616
BstDSI	CCRYGG	4 cut(s)	75, 638, 877, 1187
BstENI	CCTNNNNNAGG	1 cut(s)	252
BstF5I	GGATG	4 cut(s)	214, 667, 1393, 2154
BstFNI	CGCG	1 cut(s)	1670
BstHHI	GCGC	2 cut(s)	1112, 1981
BstKTI	GATC	8 cut(s)	137, 673, 1000, 1048, 1479, 1689, 2084, 2140
BstMAI	GTCTC	2 cut(s)	626, 1582
BstMBI	GATC	8 cut(s)	134, 670, 997, 1045, 1476, 1686, 2081, 2137
BstMCI	CGRYCG	1 cut(s)	2084
BstMWI	GCNNNNNNNGC	8 cut(s)	68, 611, 1037, 1121, 1493, 1612, 1895, 1956
BstNI	CCWGG	4 cut(s)	255, 579, 1881, 1968
BstNSI	RCATGY	4 cut(s)	874, 1505, 1726, 2001
BstPAI	GACNNNNGTC	1 cut(s)	752
BstSCI	CCNGG	7 cut(s)	253, 403, 577, 1206, 1591, 1879, 1966
BstSFI	CTRYAG	3 cut(s)	60, 615, 1221
BstSLI	GKGCMC	1 cut(s)	577
BstUI	CGCG	1 cut(s)	1670
BstV1I	GCAGC	3 cut(s)	74, 614, 1099
BstX2I	RGATCY	1 cut(s)	997
BstXI	CCANNNNNNTGG	2 cut(s)	744, 1967
BstYI	RGATCY	1 cut(s)	997
BstZ17I	GTATAC	1 cut(s)	771
BsuI	GTATCC	1 cut(s)	1964
BtgI	CCRYGG	4 cut(s)	75, 638, 877, 1187
BtsCI	GGATG	4 cut(s)	214, 667, 1393, 2154
BtsI	GCAGTG	1 cut(s)	1233
BtsIMutI	CAGTG	3 cut(s)	627, 1059, 1233
BveI	ACCTGC	3 cut(s)	695, 1188, 1478
Cac8I	GCNNGC	5 cut(s)	319, 653, 1608, 1778, 2051
CaiI	CAGNNNCTG	1 cut(s)	1790
CfoI	GCGC	2 cut(s)	1112, 1981
Cfr13I	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1740, 1820
CseI	GACGC	2 cut(s)	950, 1965
CsiI	ACCWGGT	1 cut(s)	1879
Csp6I	GTAC	5 cut(s)	874, 1341, 1408, 1855, 2068
CspCI	CAANNNNNGTGG	4 cut(s)	5, 40, 962, 997
CviQI	GTAC	5 cut(s)	874, 1341, 1408, 1855, 2068
DdeI	CTNAG	5 cut(s)	162, 531, 606, 949, 1616
DpnI	GATC	8 cut(s)	136, 672, 999, 1047, 1478, 1688, 2083, 2139
DpnII	GATC	8 cut(s)	134, 670, 997, 1045, 1476, 1686, 2081, 2137
EaeI	YGGCCR	4 cut(s)	170, 238, 921, 1038
Eco130I	CCWWGG	3 cut(s)	638, 1187, 1295
Eco147I	AGGCCT	2 cut(s)	321, 1776
Eco31I	GGTCTC	2 cut(s)	626, 1582
Eco47I	GGWCC	6 cut(s)	515, 551, 911, 1191, 1740, 1820
Eco57I	CTGAAG	3 cut(s)	51, 528, 1035
EcoNI	CCTNNNNNAGG	1 cut(s)	252
EcoO109I	RGGNCCY	2 cut(s)	515, 911
EcoRI	GAATTC	2 cut(s)	12, 776
EcoRII	CCWGG	4 cut(s)	253, 577, 1879, 1966
EcoT14I	CCWWGG	3 cut(s)	638, 1187, 1295
ErhI	CCWWGG	3 cut(s)	638, 1187, 1295
FaqI	GGGAC	4 cut(s)	620, 897, 999, 1733
FauI	CCCGC	1 cut(s)	1663
FauNDI	CATATG	1 cut(s)	349
FblI	GTMKAC	3 cut(s)	729, 770, 1288
Fnu4HI	GCNGC	5 cut(s)	63, 444, 603, 1113, 2047
FokI	GGATG	4 cut(s)	221, 674, 1400, 2161
Fsp4HI	GCNGC	5 cut(s)	63, 444, 603, 1113, 2047
FspBI	CTAG	4 cut(s)	798, 831, 1383, 1679
FspI	TGCGCA	1 cut(s)	1980
GlaI	GCGC	2 cut(s)	1111, 1980
GluI	GCNGC	5 cut(s)	63, 444, 603, 1113, 2047
GsuI	CTGGAG	5 cut(s)	219, 237, 651, 927, 953
HapII	CCGG	7 cut(s)	173, 337, 405, 1208, 1338, 1593, 2010
HgaI	GACGC	2 cut(s)	950, 1965
HhaI	GCGC	2 cut(s)	1112, 1981
Hin1I	GRCGYC	2 cut(s)	942, 1957
Hin6I	GCGC	2 cut(s)	1110, 1979
HinP1I	GCGC	2 cut(s)	1110, 1979
HincII	GTYRAC	3 cut(s)	475, 1807, 2091
HindII	GTYRAC	3 cut(s)	475, 1807, 2091
HindIII	AAGCTT	3 cut(s)	585, 1604, 1767
HinfI	GANTC	7 cut(s)	181, 378, 408, 633, 1334, 1552, 2172
HpaII	CCGG	7 cut(s)	173, 337, 405, 1208, 1338, 1593, 2010
HphI	GGTGA	2 cut(s)	1775, 2147
Hpy166II	GTNNAC	8 cut(s)	475, 575, 730, 747, 771, 1289, 1807, 2091
Hpy188I	TCNGA	7 cut(s)	186, 534, 609, 1330, 1435, 1575, 2177
Hpy188III	TCNNGA	7 cut(s)	132, 161, 337, 757, 1076, 1527, 1728
Hpy8I	GTNNAC	8 cut(s)	475, 575, 730, 747, 771, 1289, 1807, 2091
Hpy99I	CGWCG	1 cut(s)	1828
HpyCH4III	ACNGT	4 cut(s)	704, 1534, 1786, 1824
HpyCH4IV	ACGT	3 cut(s)	1349, 1578, 1850
HpyF10VI	GCNNNNNNNGC	8 cut(s)	68, 611, 1037, 1121, 1493, 1612, 1895, 1956
HpyF3I	CTNAG	5 cut(s)	162, 531, 606, 949, 1616
HpySE526I	ACGT	3 cut(s)	1349, 1578, 1850
Hsp92I	GRCGYC	2 cut(s)	942, 1957
HspAI	GCGC	2 cut(s)	1110, 1979
Kpn2I	TCCGGA	1 cut(s)	336
KpnI	GGTACC	1 cut(s)	2071
Kzo9I	GATC	8 cut(s)	134, 670, 997, 1045, 1476, 1686, 2081, 2137
LmnI	GCTCC	1 cut(s)	27
Lsp1109I	GCAGC	3 cut(s)	74, 614, 1099
LweI	GCATC	3 cut(s)	652, 1378, 1605
MabI	ACCWGGT	1 cut(s)	1879
MaeI	CTAG	4 cut(s)	798, 831, 1383, 1679
MaeII	ACGT	3 cut(s)	1349, 1578, 1850
MaeIII	GTNAC	5 cut(s)	695, 1054, 1243, 1725, 1786
MalI	GATC	8 cut(s)	136, 672, 999, 1047, 1478, 1688, 2083, 2139
MboI	GATC	8 cut(s)	134, 670, 997, 1045, 1476, 1686, 2081, 2137
MboII	GAAGA	5 cut(s)	478, 821, 1325, 1791, 2135
MflI	RGATCY	1 cut(s)	997
MhlI	GDGCHC	1 cut(s)	577
MlsI	TGGCCA	2 cut(s)	240, 923
MluNI	TGGCCA	2 cut(s)	240, 923
MlyI	GAGTC	2 cut(s)	642, 1343
MmeI	TCCRAC	2 cut(s)	1624, 1933
Mox20I	TGGCCA	2 cut(s)	240, 923
MroI	TCCGGA	1 cut(s)	336
MroXI	GAANNNNTTC	2 cut(s)	497, 1443
MscI	TGGCCA	2 cut(s)	240, 923
MseI	TTAA	3 cut(s)	105, 125, 330
Msp20I	TGGCCA	2 cut(s)	240, 923
MspI	CCGG	7 cut(s)	173, 337, 405, 1208, 1338, 1593, 2010
MspR9I	CCNGG	7 cut(s)	255, 405, 579, 1208, 1593, 1881, 1968
Mva1269I	GAATGC	3 cut(s)	30, 1115, 1200
MvaI	CCWGG	4 cut(s)	255, 579, 1881, 1968
MvnI	CGCG	1 cut(s)	1670
MwoI	GCNNNNNNNGC	8 cut(s)	68, 611, 1037, 1121, 1493, 1612, 1895, 1956
NciI	CCSGG	3 cut(s)	405, 1208, 1593
NcoI	CCATGG	2 cut(s)	638, 1187
NdeI	CATATG	1 cut(s)	349
NdeII	GATC	8 cut(s)	134, 670, 997, 1045, 1476, 1686, 2081, 2137
NlaIV	GGNNCC	3 cut(s)	913, 1531, 2069
NmuCI	GTSAC	3 cut(s)	1054, 1243, 1725
NsbI	TGCGCA	1 cut(s)	1980
NspI	RCATGY	4 cut(s)	874, 1505, 1726, 2001
NspV	TTCGAA	2 cut(s)	418, 2121
PaqCI	CACCTGC	1 cut(s)	695
PceI	AGGCCT	2 cut(s)	321, 1776
PciI	ACATGT	4 cut(s)	870, 1501, 1722, 1997
PcsI	WCGNNNNNNNCGW	1 cut(s)	1947
PctI	GAATGC	3 cut(s)	30, 1115, 1200
PdmI	GAANNNNTTC	2 cut(s)	497, 1443
PfeI	GAWTC	4 cut(s)	181, 378, 408, 1552
PflMI	CCANNNNNTGG	1 cut(s)	1283
PfoI	TCCNGGA	3 cut(s)	253, 403, 1206
PkrI	GCNGC	5 cut(s)	64, 445, 604, 1114, 2048
Ple19I	CGATCG	1 cut(s)	2084
PleI	GAGTC	2 cut(s)	641, 1342
PpsI	GAGTC	2 cut(s)	641, 1342
Ppu21I	YACGTR	1 cut(s)	1851
PpuMI	RGGWCCY	2 cut(s)	515, 911
PscI	ACATGT	4 cut(s)	870, 1501, 1722, 1997
PshAI	GACNNNNGTC	1 cut(s)	752
PshBI	ATTAAT	1 cut(s)	105
Psp5II	RGGWCCY	2 cut(s)	515, 911
Psp6I	CCWGG	4 cut(s)	253, 577, 1879, 1966
PspGI	CCWGG	4 cut(s)	253, 577, 1879, 1966
PspN4I	GGNNCC	3 cut(s)	913, 1531, 2069
PspPI	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1740, 1820
PspPPI	RGGWCCY	2 cut(s)	515, 911
PstI	CTGCAG	2 cut(s)	64, 1225
PstNI	CAGNNNCTG	1 cut(s)	1790
PsuI	RGATCY	1 cut(s)	997
PvuI	CGATCG	1 cut(s)	2084
RsaI	GTAC	5 cut(s)	875, 1342, 1409, 1856, 2069
RsaNI	GTAC	5 cut(s)	874, 1341, 1408, 1855, 2068
SaqAI	TTAA	3 cut(s)	105, 125, 330
SatI	GCNGC	5 cut(s)	63, 444, 603, 1113, 2047
Sau3AI	GATC	8 cut(s)	134, 670, 997, 1045, 1476, 1686, 2081, 2137
Sau96I	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1740, 1820
SchI	GAGTC	2 cut(s)	642, 1343
ScrFI	CCNGG	7 cut(s)	255, 405, 579, 1208, 1593, 1881, 1968
SduI	GDGCHC	1 cut(s)	577
SexAI	ACCWGGT	1 cut(s)	1879
SfaNI	GCATC	3 cut(s)	652, 1378, 1605
SfcI	CTRYAG	3 cut(s)	60, 615, 1221
SfuI	TTCGAA	2 cut(s)	418, 2121
SinI	GGWCC	6 cut(s)	515, 551, 911, 1191, 1740, 1820
SseBI	AGGCCT	2 cut(s)	321, 1776
SsiI	CCGC	5 cut(s)	443, 849, 1670, 2046, 2141
SspI	AATATT	1 cut(s)	263
SspMI	CTAG	4 cut(s)	798, 831, 1383, 1679
StuI	AGGCCT	2 cut(s)	321, 1776
StyD4I	CCNGG	7 cut(s)	253, 403, 577, 1206, 1591, 1879, 1966
StyI	CCWWGG	3 cut(s)	638, 1187, 1295
TaaI	ACNGT	4 cut(s)	704, 1534, 1786, 1824
TaiI	ACGT	3 cut(s)	1352, 1581, 1853
TaqI	TCGA	7 cut(s)	418, 807, 1311, 1826, 2080, 2121, 2160
TauI	GCSGC	2 cut(s)	446, 2049
TfiI	GAWTC	4 cut(s)	181, 378, 408, 1552
Tru1I	TTAA	3 cut(s)	105, 125, 330
Tru9I	TTAA	3 cut(s)	105, 125, 330
TscAI	CASTG	3 cut(s)	634, 1059, 1240
TseFI	GTSAC	3 cut(s)	1054, 1243, 1725
TseI	GCWGC	3 cut(s)	62, 602, 1112
Tsp45I	GTSAC	3 cut(s)	1054, 1243, 1725
TspGWI	ACGGA	4 cut(s)	64, 894, 1470, 1956
TspRI	CASTG	3 cut(s)	634, 1059, 1240
Van91I	CCANNNNNTGG	1 cut(s)	1283
VneI	GTGCAC	1 cut(s)	573
VpaK11BI	GGWCC	6 cut(s)	515, 551, 911, 1191, 1740, 1820
VspI	ATTAAT	1 cut(s)	105
XagI	CCTNNNNNAGG	1 cut(s)	252
XapI	RAATTY	5 cut(s)	12, 455, 709, 776, 889
XceI	RCATGY	4 cut(s)	874, 1505, 1726, 2001
XmiI	GTMKAC	3 cut(s)	729, 770, 1288
XmnI	GAANNNNTTC	2 cut(s)	497, 1443
XspI	CTAG	4 cut(s)	798, 831, 1383, 1679

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.