Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002889

Physical Location & Seq

Genomic Coordinates Reverse (-)

6361543 .. 6365537

Gene Structure 3995 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G006600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2262 bp

ATGGATTTAGTGCAGTCAATGTGGAACGGTAACAAGTTGAGCGCCAATGCCAACGTGTTCCTGCTATTGTTTCTGTTTTCGTGGCTGTCTTCTGCTACAGCCTCTGTGAGTTATGACCACAGAGCTATAATAATTGATGGACAGAAGAGAATTCTAATTTCTGGTTCCATCCACTATCCCAGAAGCACACCTGAGATGTGGCCTGATTTAATCCAGAAGGCTAAAGATGGAGGCTTGGATGTTATACAAACCTATGTGTTTTGGAACGGGCATGAGCCTTCTCCGGGGAAGTATTATTTTGAGGATAGATATGATTTGGCCAAGTTCATCAAGCTGGTACAAAAAGCAGGCCTGTATGTTCATCTCCGCATTGGTCCTTATGTTTGTGCTGAATGGAACTTTGGGGGATTCCCTGCTTGGCTCAAATATGTTCCGGGAATTGAGTTTAGAACAGACAATGAGCCTTTCAAAGCAGCAATGAAAACATTTACAGAGAAAATTGTCGGAATGATGAAGGCAGAACGACTGTTCCAAACTCAGGGAGGTCCCATTATTCTGTCTCAGATAGAAAATGAGTATGGACCGGTGGAATGGACAATCGGCGCCCCTGGTAAAGCATATACCAATTGGACAGCACAAATGGCTCTTGGGCTTGACACTGGAGTCCCATGGGTAATGTGCAAGCAAGAGGATGCTCCTGACCCAATTATCGATACTTGCAACGGTTTCTACTGTGAAAACTTCACTCCAAACAAGAAGTATAAACCCAAAATGTGGACCGAAGTGTGGACTGGCTGGTATACAGAATTTGGCGGGGCAGTTCCTACTAGACCTGCACAAGATTTGGCATTTTCAGTTGCAAGGTTTATACAAAATGGTGGCTCTTTTGCTAACTATTACATGTATCATGGAGGAACTAATTTTGACCGAACTTCTGGCCTTTTCGTTGCAACTAGCTATGACTATGATGCACCTCTAGATGAATATGGACTACCAAGGGAACCAAAGTGGAGTCATCTGAAATATTTGCACAAAGCCATCAAATTGGCTGAGGCAGCTTTAGTGGCTACTGATCCTGCAGTGACATCACTTGGAAAGAGTCAAGAGGCTCATGTATTCAATTCAAGTTCTGGGTGTGCTGCATTTCTTGCAAATTACGACACAAAATACTCGTCAAGAGTGAGCTTTGGGAATAGGCAATATAAGCTGCCACCCTGGTCTATAAGCATTCTTCCTGACTGCAAAACTGAAGTTCTCAACACTGGAAGGGTTGGCCAGACCCCGCCGGTGGATATGACACCAGTAAATACCACCCTTTCTTGGCAGTCGTACATCGAAGATGCTGCCTCTTCTACTGATGACAATACATTTACATTGGATGGGCTCTATGAGCAAATAAATATCACTAGGGATGCAACAGACTACTTATGGTACATGACAGACATCACAATAGGTCCTAATGAAGAGTTTCTAAAGAATGGATCTTACCCTCTTCTGACTATCGATTCAGCTGGACATGCCTTGCATGTTTTCATCAATGGTCAACTATCAGGAACTGTTTATGCAGGATTGGCATCTCCTAAATTAAGATTTAATGACACTGTGAAGCTCAGAGCTGGCATCAACAAGCTTGCTTTACTAAACATTGCTGTTGGTCTGCCGAATGTTGGTGTGCACTTTGAGACTTGGAATGCTGGAGTTCTAGGGCCGGTCACATTGAAGGGTCTGAATTCGGGAACATGGGACATGTCCCAGTGGAAATGGACCTACAAGACTGGTATGAAAGGTGAAGCATTAAGCCTCCATACTGTTAAGGGAAGTTCTTCCGTTGAATGGGTAGAAGGACCACTATTGGCTCAAAAACAACCCCTTACGTGGTACAAGTCTATATTTCATGCACCACCGGGAAATGCTCCATTAGCTTTAGATATGGTTAGCATGGGCAAGGGTCAAGTATGGATCAATGGTCAGAGCATCGGACGCCACTGGCCTGGATACATAGCACATGGTAGCTGTGACAATTGTTCTTATGCTGGAACTTACACCGAAAACAAATGCCGAACAAATTGTGGTCAGCCGTCACAGAGATGGTACCATGTTCCACAATCGTGGTTGAACCAGAGTGAAAATTTGTTGGTGGTGTTTGAAGAATGGGGTGGTGACCCGACTAAGATTTCTCTGGTGGCACGAAGCTATGAAAACCTAGGGCAGACCCTCTATAACATAGAGGGCGGCTCCAACCATTTGAGATTGCAATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

753

Amino Acids

84.08

Weight (kDa)

6.3

Isoelectric Point (pI)

37.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	43 - 346	3.6e-117	Glycosyl hydrolases family 35
GHD	PF17834	369 - 424	1e-19	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	475 - 523	5.6e-06	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	624 - 710	2.3e-24	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56870
fragaria_vesca	FvH4_1g07401 FvH4_1g07410 FvH4_1g07410
malus_domestica	MD02G1079200.v1.1 MD08G1139000.v1.1 MD13G1126600.v1.1 MD15G1206800.v1.1
prunus_persica	Prupe.7G210000_v2.0.a1 Prupe.7G210000_v2.0.a1
pyrus_communis	pycom02g06300 pycom15g18320
rosa_chinensis	RchiOBHm_Chr2g0093301 RchiOBHm_Chr2g0093311 RchiOBHm_Chr3g0459401 RchiOBHm_Chr3g0459561
rosa_laevigata	RLG00000016388 RLG00000016389 RLG00000025083
rosa_multiflora	Rmu_sc0002089.1_g000005
rosa_roxburghii	Rroxscaffold_2G00148200 Rroxscaffold_2G00148210 Rroxscaffold_5G00345960
rosa_rugosa	Rorug02G0034700.1 Rorug03G0036300
rosa_samantha	Rh2AG080700 Rh2AG080800 Rh2CG084000 Rh2DG079700 Rh3AG094200 Rh3BG097100 Rh3CG098400 Rh3DG097900 Rh3DG098900
rosa_wichuraiana	Rw2G006600 Rw2G006610 Rw3G008110 Rw3G008200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	662
Acc36I	ACCTGC	1 cut(s)	841
Acc65I	GGTACC	1 cut(s)	2091
AccB1I	GGYRCC	2 cut(s)	602, 2091
AccB7I	CCANNNNNTGG	1 cut(s)	774
AccI	GTMKAC	1 cut(s)	800
AciI	CCGC	4 cut(s)	367, 813, 1283, 2232
AclWI	GGATC	3 cut(s)	1067, 1489, 1967
AcoI	YGGCCR	2 cut(s)	318, 1273
AcsI	RAATTY	4 cut(s)	150, 806, 1729, 2128
AcuI	CTGAAG	1 cut(s)	1269
AcyI	GRCGYC	2 cut(s)	603, 1981
AfaI	GTAC	5 cut(s)	339, 1331, 1433, 1880, 2093
AfiI	CCNNNNNNNGG	9 cut(s)	284, 538, 774, 786, 1288, 1320, 1667, 1833, 1875
AflIII	ACRYGT	3 cut(s)	54, 900, 1746
AgeI	ACCGGT	1 cut(s)	583
AgsI	TTSAA	7 cut(s)	469, 1120, 1125, 1720, 1832, 2116, 2147
AjnI	CCWGG	3 cut(s)	607, 1214, 1990
AjuI	GAANNNNNNNTTGG	2 cut(s)	384, 416
AleI	CACNNNNGTG	1 cut(s)	2107
AloI	GAACNNNNNNTCC	2 cut(s)	513, 545
Alw21I	GWGCWC	1 cut(s)	1677
Alw26I	GTCTC	2 cut(s)	564, 1676
Alw44I	GTGCAC	1 cut(s)	1673
AlwI	GGATC	3 cut(s)	1067, 1489, 1967
AlwNI	CAGNNNCTG	2 cut(s)	104, 1556
AoxI	GGCC	7 cut(s)	200, 318, 349, 937, 1273, 1706, 1988
ApaLI	GTGCAC	1 cut(s)	1673
ApeKI	GCWGC	5 cut(s)	473, 1055, 1139, 1207, 1343
ApoI	RAATTY	4 cut(s)	150, 806, 1729, 2128
ArsI	GACNNNNNNTTYG	2 cut(s)	1157, 1189
AsiGI	ACCGGT	1 cut(s)	583
Asp700I	GAANNNNTTC	2 cut(s)	1467, 1822
Asp718I	GGTACC	1 cut(s)	2091
AspA2I	CCTAGG	1 cut(s)	2203
AspLEI	GCGC	2 cut(s)	44, 605
AspS9I	GGNCC	8 cut(s)	374, 545, 581, 777, 1454, 1706, 1764, 1844
AsuC2I	CCSGG	3 cut(s)	285, 435, 1905
AsuHPI	GGTGA	2 cut(s)	1799, 2171
AvaII	GGWCC	7 cut(s)	374, 545, 581, 777, 1454, 1764, 1844
AvrII	CCTAGG	1 cut(s)	2203
BaeGI	GKGCMC	1 cut(s)	1677
BalI	TGGCCA	2 cut(s)	320, 1275
BanI	GGYRCC	2 cut(s)	602, 2091
BanII	GRGCYC	1 cut(s)	1386
BbsI	GAAGAC	1 cut(s)	81
Bbv12I	GWGCWC	1 cut(s)	1677
BbvCI	CCTCAGC	1 cut(s)	1050
BbvI	GCAGC	5 cut(s)	485, 1067, 1126, 1194, 1330
BccI	CCATC	6 cut(s)	131, 176, 221, 1046, 1373, 2082
BceAI	ACGGC	1 cut(s)	2062
BcgI	CGANNNNNNTGC	2 cut(s)	1325, 1359
BciT130I	CCWGG	3 cut(s)	609, 1216, 1992
BciVI	GTATCC	1 cut(s)	1988
BcnI	CCSGG	3 cut(s)	285, 435, 1905
BcoDI	GTCTC	2 cut(s)	564, 1676
BfaI	CTAG	6 cut(s)	828, 954, 977, 1407, 1703, 2204
BfmI	CTRYAG	2 cut(s)	96, 1077
BfoI	RGCGCY	2 cut(s)	45, 606
BfuAI	ACCTGC	1 cut(s)	841
BfuI	GTATCC	1 cut(s)	1988
BisI	GCNGC	6 cut(s)	474, 1056, 1140, 1208, 1344, 2233
BlnI	CCTAGG	1 cut(s)	2203
BlsI	GCNGC	6 cut(s)	475, 1057, 1141, 1209, 1345, 2234
Bme1390I	CCNGG	6 cut(s)	285, 435, 609, 1216, 1905, 1992
Bme18I	GGWCC	7 cut(s)	374, 545, 581, 777, 1454, 1764, 1844
BmgT120I	GGNCC	8 cut(s)	374, 545, 581, 777, 1454, 1706, 1764, 1844
BmiI	GGNNCC	6 cut(s)	166, 547, 604, 1002, 2093, 2236
BmrFI	CCNGG	6 cut(s)	285, 435, 609, 1216, 1905, 1992
BmrI	ACTGGG	1 cut(s)	1747
BmsI	GCATC	7 cut(s)	682, 958, 1330, 1402, 1583, 1629, 1983
BmuI	ACTGGG	1 cut(s)	1747
BpiI	GAAGAC	1 cut(s)	81
BplI	GAGNNNNNCTC	2 cut(s)	2219, 2251
BpmI	CTGGAG	2 cut(s)	681, 1716
Bpu10I	CCTNAGC	1 cut(s)	1050
BpuMI	CCSGG	3 cut(s)	285, 435, 1905
Bsa29I	ATCGAT	2 cut(s)	711, 1503
BsaAI	YACGTR	1 cut(s)	1875
BsaBI	GATNNNNATC	1 cut(s)	1573
BsaHI	GRCGYC	2 cut(s)	603, 1981
BsaJI	CCNNGG	6 cut(s)	284, 607, 668, 995, 1214, 2203
BsaWI	WCCGGW	1 cut(s)	583
Bsc4I	CCNNNNNNNGG	9 cut(s)	284, 538, 774, 786, 1288, 1320, 1667, 1833, 1875
Bse118I	RCCGGY	3 cut(s)	583, 1285, 1708
Bse1I	ACTGG	7 cut(s)	664, 796, 1267, 1301, 1753, 1780, 1991
Bse3DI	GCAATG	2 cut(s)	483, 1644
Bse8I	GATNNNNATC	1 cut(s)	1573
BseBI	CCWGG	3 cut(s)	609, 1216, 1992
BseCI	ATCGAT	2 cut(s)	711, 1503
BseDI	CCNNGG	6 cut(s)	284, 607, 668, 995, 1214, 2203
BseGI	GGATG	5 cut(s)	168, 244, 697, 1384, 1417
BseJI	GATNNNNATC	1 cut(s)	1573
BseLI	CCNNNNNNNGG	9 cut(s)	284, 538, 774, 786, 1288, 1320, 1667, 1833, 1875
BseMI	GCAATG	2 cut(s)	483, 1644
BseMII	CTCAG	5 cut(s)	183, 551, 575, 1041, 1624
BseNI	ACTGG	7 cut(s)	664, 796, 1267, 1301, 1753, 1780, 1991
BseSI	GKGCMC	1 cut(s)	1677
BseXI	GCAGC	5 cut(s)	485, 1067, 1126, 1194, 1330
BsgI	GTGCAG	2 cut(s)	32, 819
BshFI	GGCC	7 cut(s)	202, 320, 351, 939, 1275, 1708, 1990
BshNI	GGYRCC	2 cut(s)	602, 2091
BshTI	ACCGGT	1 cut(s)	583
BshVI	ATCGAT	2 cut(s)	711, 1503
BsiHKAI	GWGCWC	1 cut(s)	1677
BsiSI	CCGG	6 cut(s)	284, 434, 584, 1286, 1709, 1904
BslFI	GGGAC	4 cut(s)	531, 650, 1735, 1757
BslI	CCNNNNNNNGG	9 cut(s)	284, 538, 774, 786, 1288, 1320, 1667, 1833, 1875
BsmAI	GTCTC	2 cut(s)	564, 1676
BsmFI	GGGAC	4 cut(s)	531, 650, 1735, 1757
BsmI	GAATGC	2 cut(s)	1227, 1696
BsnI	GGCC	7 cut(s)	202, 320, 351, 939, 1275, 1708, 1990
Bsp1286I	GDGCHC	2 cut(s)	1386, 1677
Bsp143I	GATC	3 cut(s)	1072, 1481, 1959
Bsp19I	CCATGG	1 cut(s)	668
BspACI	CCGC	4 cut(s)	367, 813, 1283, 2232
BspANI	GGCC	7 cut(s)	202, 320, 351, 939, 1275, 1708, 1990
BspCNI	CTCAG	5 cut(s)	184, 550, 574, 1042, 1623
BspDI	ATCGAT	2 cut(s)	711, 1503
BspLI	GGNNCC	6 cut(s)	166, 547, 604, 1002, 2093, 2236
BspMAI	CTGCAG	1 cut(s)	1081
BspMI	ACCTGC	1 cut(s)	841
BspPI	GGATC	3 cut(s)	1067, 1489, 1967
BspT107I	GGYRCC	2 cut(s)	602, 2091
BsrDI	GCAATG	2 cut(s)	483, 1644
BsrFI	RCCGGY	3 cut(s)	583, 1285, 1708
BsrI	ACTGG	7 cut(s)	664, 796, 1267, 1301, 1753, 1780, 1991
BssAI	RCCGGY	3 cut(s)	583, 1285, 1708
BssECI	CCNNGG	6 cut(s)	284, 607, 668, 995, 1214, 2203
BssMI	GATC	3 cut(s)	1072, 1481, 1959
BssNAI	GTATAC	1 cut(s)	801
BssNI	GRCGYC	2 cut(s)	603, 1981
BssT1I	CCWWGG	3 cut(s)	668, 995, 2203
Bst1107I	GTATAC	1 cut(s)	801
Bst2UI	CCWGG	3 cut(s)	609, 1216, 1992
Bst4CI	ACNGT	7 cut(s)	29, 528, 725, 734, 1558, 1603, 1810
Bst6I	CTCTTC	4 cut(s)	140, 1354, 1458, 1497
BstACI	GRCGYC	2 cut(s)	603, 1981
BstAPI	GCANNNNNTGC	1 cut(s)	1148
BstBAI	YACGTR	1 cut(s)	1875
BstC8I	GCNNGC	4 cut(s)	349, 683, 1618, 1632
BstDEI	CTNAG	6 cut(s)	192, 537, 561, 1050, 1610, 2169
BstDSI	CCRYGG	1 cut(s)	668
BstEII	GGTNACC	1 cut(s)	2159
BstF5I	GGATG	5 cut(s)	168, 244, 697, 1384, 1417
BstH2I	RGCGCY	2 cut(s)	45, 606
BstHHI	GCGC	2 cut(s)	44, 605
BstKTI	GATC	3 cut(s)	1075, 1484, 1962
BstMAI	GTCTC	2 cut(s)	564, 1676
BstMBI	GATC	3 cut(s)	1072, 1481, 1959
BstNI	CCWGG	3 cut(s)	609, 1216, 1992
BstNSI	RCATGY	4 cut(s)	904, 1520, 1529, 1750
BstPI	GGTNACC	1 cut(s)	2159
BstSCI	CCNGG	6 cut(s)	283, 433, 607, 1214, 1903, 1990
BstSFI	CTRYAG	2 cut(s)	96, 1077
BstSLI	GKGCMC	1 cut(s)	1677
BstV1I	GCAGC	5 cut(s)	485, 1067, 1126, 1194, 1330
BstV2I	GAAGAC	1 cut(s)	81
BstX2I	RGATCY	1 cut(s)	1481
BstXI	CCANNNNNNTGG	3 cut(s)	1045, 1991, 2109
BstYI	RGATCY	1 cut(s)	1481
BstZ17I	GTATAC	1 cut(s)	801
Bsu15I	ATCGAT	2 cut(s)	711, 1503
BsuI	GTATCC	1 cut(s)	1988
BsuRI	GGCC	7 cut(s)	202, 320, 351, 939, 1275, 1708, 1990
BsuTUI	ATCGAT	2 cut(s)	711, 1503
BtgI	CCRYGG	1 cut(s)	668
BtsCI	GGATG	5 cut(s)	168, 244, 697, 1384, 1417
BtsI	GCAGTG	1 cut(s)	1086
BtsIMutI	CAGTG	6 cut(s)	657, 1086, 1260, 1599, 1760, 1984
BveI	ACCTGC	1 cut(s)	841
Cac8I	GCNNGC	4 cut(s)	349, 683, 1618, 1632
CaiI	CAGNNNCTG	2 cut(s)	104, 1556
CfoI	GCGC	2 cut(s)	44, 605
Cfr10I	RCCGGY	3 cut(s)	583, 1285, 1708
Cfr13I	GGNCC	8 cut(s)	374, 545, 581, 777, 1454, 1706, 1764, 1844
ClaI	ATCGAT	2 cut(s)	711, 1503
CseI	GACGC	1 cut(s)	1989
Csp6I	GTAC	5 cut(s)	338, 1330, 1432, 1879, 2092
CspAI	ACCGGT	1 cut(s)	583
CviQI	GTAC	5 cut(s)	338, 1330, 1432, 1879, 2092
DdeI	CTNAG	6 cut(s)	192, 537, 561, 1050, 1610, 2169
DinI	GGCGCC	1 cut(s)	604
DpnI	GATC	3 cut(s)	1074, 1483, 1961
DpnII	GATC	3 cut(s)	1072, 1481, 1959
DrdI	GACNNNNNNGTC	1 cut(s)	662
DseDI	GACNNNNNNGTC	1 cut(s)	662
EaeI	YGGCCR	2 cut(s)	318, 1273
Eam1104I	CTCTTC	4 cut(s)	140, 1354, 1458, 1497
EarI	CTCTTC	4 cut(s)	140, 1354, 1458, 1497
Eco130I	CCWWGG	3 cut(s)	668, 995, 2203
Eco147I	AGGCCT	1 cut(s)	351
Eco24I	GRGCYC	1 cut(s)	1386
Eco47I	GGWCC	7 cut(s)	374, 545, 581, 777, 1454, 1764, 1844
Eco57I	CTGAAG	1 cut(s)	1269
Eco91I	GGTNACC	1 cut(s)	2159
EcoO109I	RGGNCCY	2 cut(s)	545, 1454
EcoO65I	GGTNACC	1 cut(s)	2159
EcoRI	GAATTC	2 cut(s)	150, 1729
EcoRII	CCWGG	3 cut(s)	607, 1214, 1990
EcoT14I	CCWWGG	3 cut(s)	668, 995, 2203
EcoT38I	GRGCYC	1 cut(s)	1386
EgeI	GGCGCC	1 cut(s)	604
EheI	GGCGCC	1 cut(s)	604
ErhI	CCWWGG	3 cut(s)	668, 995, 2203
FaqI	GGGAC	4 cut(s)	531, 650, 1735, 1757
FauI	CCCGC	2 cut(s)	806, 1290
FblI	GTMKAC	1 cut(s)	800
Fnu4HI	GCNGC	6 cut(s)	474, 1056, 1140, 1208, 1344, 2233
FokI	GGATG	5 cut(s)	155, 251, 704, 1391, 1424
FriOI	GRGCYC	1 cut(s)	1386
Fsp4HI	GCNGC	6 cut(s)	474, 1056, 1140, 1208, 1344, 2233
FspBI	CTAG	6 cut(s)	828, 954, 977, 1407, 1703, 2204
GlaI	GCGC	2 cut(s)	43, 604
GluI	GCNGC	6 cut(s)	474, 1056, 1140, 1208, 1344, 2233
GsuI	CTGGAG	2 cut(s)	681, 1716
HaeII	RGCGCY	2 cut(s)	45, 606
HaeIII	GGCC	7 cut(s)	202, 320, 351, 939, 1275, 1708, 1990
HapII	CCGG	6 cut(s)	284, 434, 584, 1286, 1709, 1904
HgaI	GACGC	1 cut(s)	1989
HhaI	GCGC	2 cut(s)	44, 605
Hin1I	GRCGYC	2 cut(s)	603, 1981
Hin6I	GCGC	2 cut(s)	42, 603
HinP1I	GCGC	2 cut(s)	42, 603
HincII	GTYRAC	1 cut(s)	1544
HindII	GTYRAC	1 cut(s)	1544
HindIII	AAGCTT	1 cut(s)	1628
HinfI	GANTC	5 cut(s)	408, 663, 1012, 1099, 1505
HpaII	CCGG	6 cut(s)	284, 434, 584, 1286, 1709, 1904
HphI	GGTGA	2 cut(s)	1799, 2171
Hpy166II	GTNNAC	5 cut(s)	777, 789, 801, 1544, 1675
Hpy188I	TCNGA	8 cut(s)	506, 564, 1020, 1497, 1613, 1728, 1971, 1979
Hpy188III	TCNNGA	8 cut(s)	214, 698, 977, 1103, 1176, 1235, 1551, 1734
Hpy8I	GTNNAC	5 cut(s)	777, 789, 801, 1544, 1675
HpyAV	CCTTC	6 cut(s)	211, 288, 508, 1260, 1714, 1835
HpyCH4III	ACNGT	7 cut(s)	29, 528, 725, 734, 1558, 1603, 1810
HpyCH4IV	ACGT	2 cut(s)	54, 1874
HpyF3I	CTNAG	6 cut(s)	192, 537, 561, 1050, 1610, 2169
HpySE526I	ACGT	2 cut(s)	54, 1874
Hsp92I	GRCGYC	2 cut(s)	603, 1981
HspAI	GCGC	2 cut(s)	42, 603
KasI	GGCGCC	1 cut(s)	602
KpnI	GGTACC	1 cut(s)	2095
Kzo9I	GATC	3 cut(s)	1072, 1481, 1959
LmnI	GCTCC	3 cut(s)	700, 1918, 2240
Lsp1109I	GCAGC	5 cut(s)	485, 1067, 1126, 1194, 1330
LweI	GCATC	7 cut(s)	682, 958, 1330, 1402, 1583, 1629, 1983
MaeI	CTAG	6 cut(s)	828, 954, 977, 1407, 1703, 2204
MaeII	ACGT	2 cut(s)	54, 1874
MaeIII	GTNAC	6 cut(s)	29, 1081, 1711, 2015, 2079, 2159
MalI	GATC	3 cut(s)	1074, 1483, 1961
MboI	GATC	3 cut(s)	1072, 1481, 1959
MboII	GAAGA	9 cut(s)	81, 157, 1223, 1341, 1349, 1475, 1484, 1815, 2159
MfeI	CAATTG	3 cut(s)	625, 2020, 2255
MflI	RGATCY	1 cut(s)	1481
MhlI	GDGCHC	2 cut(s)	1386, 1677
MlsI	TGGCCA	2 cut(s)	320, 1275
MluNI	TGGCCA	2 cut(s)	320, 1275
Mly113I	GGCGCC	1 cut(s)	603
MlyI	GAGTC	3 cut(s)	672, 1021, 1108
MmeI	TCCRAC	2 cut(s)	484, 2262
Mox20I	TGGCCA	2 cut(s)	320, 1275
MroXI	GAANNNNTTC	2 cut(s)	1467, 1822
MscI	TGGCCA	2 cut(s)	320, 1275
MseI	TTAA	5 cut(s)	209, 1586, 1593, 1796, 1812
MslI	CAYNNNNRTG	2 cut(s)	2086, 2107
Msp20I	TGGCCA	2 cut(s)	320, 1275
MspA1I	CMGCKG	1 cut(s)	1511
MspI	CCGG	6 cut(s)	284, 434, 584, 1286, 1709, 1904
MspR9I	CCNGG	6 cut(s)	285, 435, 609, 1216, 1905, 1992
MunI	CAATTG	3 cut(s)	625, 2020, 2255
Mva1269I	GAATGC	2 cut(s)	1227, 1696
MvaI	CCWGG	3 cut(s)	609, 1216, 1992
NarI	GGCGCC	1 cut(s)	603
NciI	CCSGG	3 cut(s)	285, 435, 1905
NcoI	CCATGG	1 cut(s)	668
NdeII	GATC	3 cut(s)	1072, 1481, 1959
NlaIV	GGNNCC	6 cut(s)	166, 547, 604, 1002, 2093, 2236
NmuCI	GTSAC	5 cut(s)	1081, 1711, 2015, 2079, 2159
NspI	RCATGY	4 cut(s)	904, 1520, 1529, 1750
OliI	CACNNNNGTG	1 cut(s)	2107
PceI	AGGCCT	1 cut(s)	351
PciI	ACATGT	2 cut(s)	900, 1746
PctI	GAATGC	2 cut(s)	1227, 1696
PdmI	GAANNNNTTC	2 cut(s)	1467, 1822
PfeI	GAWTC	2 cut(s)	408, 1505
PflMI	CCANNNNNTGG	1 cut(s)	774
PfoI	TCCNGGA	1 cut(s)	433
PinAI	ACCGGT	1 cut(s)	583
PkrI	GCNGC	6 cut(s)	475, 1057, 1141, 1209, 1345, 2234
PleI	GAGTC	3 cut(s)	671, 1020, 1107
PluTI	GGCGCC	1 cut(s)	606
PpsI	GAGTC	3 cut(s)	671, 1020, 1107
Ppu21I	YACGTR	1 cut(s)	1875
PpuMI	RGGWCCY	2 cut(s)	545, 1454
PscI	ACATGT	2 cut(s)	900, 1746
Psp5II	RGGWCCY	2 cut(s)	545, 1454
Psp6I	CCWGG	3 cut(s)	607, 1214, 1990
PspEI	GGTNACC	1 cut(s)	2159
PspGI	CCWGG	3 cut(s)	607, 1214, 1990
PspN4I	GGNNCC	6 cut(s)	166, 547, 604, 1002, 2093, 2236
PspPI	GGNCC	8 cut(s)	374, 545, 581, 777, 1454, 1706, 1764, 1844
PspPPI	RGGWCCY	2 cut(s)	545, 1454
PstI	CTGCAG	1 cut(s)	1081
PstNI	CAGNNNCTG	2 cut(s)	104, 1556
PsuI	RGATCY	1 cut(s)	1481
PvuII	CAGCTG	1 cut(s)	1511
RsaI	GTAC	5 cut(s)	339, 1331, 1433, 1880, 2093
RsaNI	GTAC	5 cut(s)	338, 1330, 1432, 1879, 2092
RseI	CAYNNNNRTG	2 cut(s)	2086, 2107
SaqAI	TTAA	5 cut(s)	209, 1586, 1593, 1796, 1812
SatI	GCNGC	6 cut(s)	474, 1056, 1140, 1208, 1344, 2233
Sau3AI	GATC	3 cut(s)	1072, 1481, 1959
Sau96I	GGNCC	8 cut(s)	374, 545, 581, 777, 1454, 1706, 1764, 1844
SchI	GAGTC	3 cut(s)	672, 1021, 1108
ScrFI	CCNGG	6 cut(s)	285, 435, 609, 1216, 1905, 1992
SduI	GDGCHC	2 cut(s)	1386, 1677
SfaNI	GCATC	7 cut(s)	682, 958, 1330, 1402, 1583, 1629, 1983
SfcI	CTRYAG	2 cut(s)	96, 1077
SfoI	GGCGCC	1 cut(s)	604
SgrAI	CRCCGGYG	1 cut(s)	1285
SinI	GGWCC	7 cut(s)	374, 545, 581, 777, 1454, 1764, 1844
SmiMI	CAYNNNNRTG	2 cut(s)	2086, 2107
SseBI	AGGCCT	1 cut(s)	351
SsiI	CCGC	4 cut(s)	367, 813, 1283, 2232
SspDI	GGCGCC	1 cut(s)	602
SspI	AATATT	1 cut(s)	1025
SspMI	CTAG	6 cut(s)	828, 954, 977, 1407, 1703, 2204
StuI	AGGCCT	1 cut(s)	351
StyD4I	CCNGG	6 cut(s)	283, 433, 607, 1214, 1903, 1990
StyI	CCWWGG	3 cut(s)	668, 995, 2203
TaaI	ACNGT	7 cut(s)	29, 528, 725, 734, 1558, 1603, 1810
TaiI	ACGT	2 cut(s)	57, 1877
TaqI	TCGA	3 cut(s)	711, 1335, 1503
TaqII	GACCGA	2 cut(s)	794, 942
TauI	GCSGC	1 cut(s)	2235
TfiI	GAWTC	2 cut(s)	408, 1505
Tru1I	TTAA	5 cut(s)	209, 1586, 1593, 1796, 1812
Tru9I	TTAA	5 cut(s)	209, 1586, 1593, 1796, 1812
TscAI	CASTG	6 cut(s)	664, 1086, 1267, 1606, 1760, 1991
TseFI	GTSAC	5 cut(s)	1081, 1711, 2015, 2079, 2159
TseI	GCWGC	5 cut(s)	473, 1055, 1139, 1207, 1343
Tsp45I	GTSAC	5 cut(s)	1081, 1711, 2015, 2079, 2159
TspGWI	ACGGA	1 cut(s)	1816
TspRI	CASTG	6 cut(s)	664, 1086, 1267, 1606, 1760, 1991
Van91I	CCANNNNNTGG	1 cut(s)	774
VneI	GTGCAC	1 cut(s)	1673
VpaK11BI	GGWCC	7 cut(s)	374, 545, 581, 777, 1454, 1764, 1844
XapI	RAATTY	4 cut(s)	150, 806, 1729, 2128
XbaI	TCTAGA	1 cut(s)	976
XceI	RCATGY	4 cut(s)	904, 1520, 1529, 1750
XmaJI	CCTAGG	1 cut(s)	2203
XmiI	GTMKAC	1 cut(s)	800
XmnI	GAANNNNTTC	2 cut(s)	1467, 1822
XspI	CTAG	6 cut(s)	828, 954, 977, 1407, 1703, 2204

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.