Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002889

Physical Location & Seq

Genomic Coordinates Forward (+)

6199106 .. 6203512

Gene Structure 4407 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016388

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2178 bp

ATGTGGAACTCTAACATGTTAAGGGCCAAGGCCAACATGTTCTTCCTACTGTTTCTGGTTTCATGGCTGTCTTCCGCTTTGGCCTCAGTGGGTTATGACCACAGGGCTGTTATAGTCAATGGAAAGAGAAGGATTCTCCTTTCTGGGTCTATTCACTACCCCAGAAGCACACCTGAGATGTGGCCGGATTTGCTTCAGAAGGCCAAAGATGGAGGCTTGGATGTGATACAGACCTATGTCTTCTGGAATGGGCATGAACCAAGCCCAGGAAAATATTATTTTGAGGATAGATATGATCTGGTCAAGTTCATCAAGCTGGCTCAACAACATGGCCTATATGTTCATCTCCGGATTGGCCCTTATATTTGTGCTGAATGGAACTTTGGAGGATTCCCTGTTTGGCTGAAATATGTTCCCGGAATGGTTTTCAGAACAGACAATCGTCCTTTCATGGCAGCAATGGAAAAATTTACACAGAAGATTGTGTATATGATGAAGGCAGAAAGGTTGTTCCAAACTCAGGGAGGTCCTATAATTCTGTCTCAGATTGAAAATGAATTTGGACCGGTGGAGTGGGAAATTGGTGCACCTGGTAAATCTTATACTCAATGGGCAGCTAAAATGGCTGTGGGTCTCAACACTGGGGTTCCATGGGTTATGTGCAAGCAAGAAGATGCCCCTGACCCAATTATCGATACTTGCAATGGCTTCTACTGTGAAAACTTCACTCCAAACAAGAACTATAAGCCCAAAATGTGGACAGAAATCTGGACTGGCTGGTATACAGAATTTGGTGGGGCAGTTCCTACTAGACCTGCACAGGACTTGGCCTTTTCAGTTGCAAGGTTTATACAAAATGGTGGTTCTTTTGCCAACTACTACATGTACCATGGGGGAACAAATTTTGGCCGAACTGCTGGCGGTCCCTTCATTGCAACTAGCTATGACTATGATGCACCTCTAGATGAATATGGACTGCCAAGGGAACCAAAATATAGCCATTTGAAATATCTGCACAAAGCCATCAAATTAGCTGAGCCAGCTTTAGTGGCTACTGATGCCGCAGTGTCAAAACTTGGAAATAATCAAGAGGCTCATGTATACCAGTCAGGATCTGGTTGTGCTGCATTCCTTGCAAATTACGACACAAAATACTCCGTGAGAGTGACATTTTGGAATAAGCCGTATAACCTGCCACCTTGGTCCATAAGCATTCTTCCTGATTGCAAAACTGAAGTTTTCAACACTGCAAGGGTTGGCCAGAGCCCGCCAACGAAGATGACACCAGTAAGTCGCCTTTCTTGGCAAGCTTACATCGAAGAAGTTGCCACTTCTACTGATGAGAAGGCATTTACATTGGTCGGACTTCGTGAGCAAATAAGTCTCACTAGGGATGCTACAGACTACTTGTGGTACATGACAGATATCACAATAGGTCCTAATGAACAATTTTTAAGAACTGGAAAGTACCCCACTCTCAAAGTCGATTCAGCTGGCCATGCCTTGCATGTTTTCATCAATGGCCAACTATCTGGATCTGCTTATGGATCATTGGAGTCCCCCAAGTTAGAATTTAATCAGGGTGTGAAGCTGAGAGCTGGCATTAACAAGCTTGCTTTACTTAGCGCTTCTGTTGGTCTGCAGAATGTTGGTGTGCACTTTGAGACATGGAATGCTGGAGTTCTTGGGCCGGTCACATTGACGGGTGTGAATTCGGGAACATGGGATATGTCACGATGGCAATGGACCCACAAGATTGGTATGAAAGGTGAAGATATGAGCCTCCATACCATTAGTGGAAGTTCTTCTGTTGAATGGGTACAAGGATCACTATTGGCTCAAAATCGACCCCTTACATGGTACAAGGCTATTTTAAGTGCACCACCAGGAAATGCACCATTAGCTTTAGATATGGGTAGCATGGGAAAGGGTCAAATGTGGATTAATGGTCAGAGCATCGGACGCCATTGGCCGGCATATATAGCACGCGGTAGTTGTGGTGCTTGTTATTATGCTGGAACTTATACCGAAAACAAATGCCGAACAAATTGTGGCCAGCCCTCTCAGAGATGGTACCATGTTCCCCGCTCGTGGTTGAAGCCAAGTGGGAATTTATTGGTTGTGTTTGAAGAATGGGGTGGTGATCCGACTAAGATTGCTTTGGTAGCAAGAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

726

Amino Acids

81.15

Weight (kDa)

8.84

Isoelectric Point (pI)

40.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	37 - 341	2.5e-120	Glycosyl hydrolases family 35
GHD	PF17834	364 - 419	5.1e-23	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	469 - 522	1.4e-06	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	618 - 704	2.1e-24	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56870
fragaria_vesca	FvH4_1g07401 FvH4_1g07410 FvH4_1g07410
malus_domestica	MD02G1079200.v1.1 MD08G1139000.v1.1 MD13G1126600.v1.1 MD15G1206800.v1.1
prunus_persica	Prupe.7G210000_v2.0.a1 Prupe.7G210000_v2.0.a1
pyrus_communis	pycom02g06300 pycom15g18320
rosa_chinensis	RchiOBHm_Chr2g0093301 RchiOBHm_Chr2g0093311 RchiOBHm_Chr3g0459401 RchiOBHm_Chr3g0459561
rosa_laevigata	RLG00000016388 RLG00000016389 RLG00000025083
rosa_multiflora	Rmu_sc0002089.1_g000005
rosa_roxburghii	Rroxscaffold_2G00148200 Rroxscaffold_2G00148210 Rroxscaffold_5G00345960
rosa_rugosa	Rorug02G0034700.1 Rorug03G0036300
rosa_samantha	Rh2AG080700 Rh2AG080800 Rh2CG084000 Rh2DG079700 Rh3AG094200 Rh3BG097100 Rh3CG098400 Rh3DG097900 Rh3DG098900
rosa_wichuraiana	Rw2G006600 Rw2G006610 Rw3G008110 Rw3G008200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	823, 1200
Acc65I	GGTACC	1 cut(s)	2073
AccB1I	GGYRCC	1 cut(s)	2073
AccB7I	CCANNNNNTGG	1 cut(s)	756
AccBSI	CCGCTC	1 cut(s)	2088
AccI	GTMKAC	2 cut(s)	782, 1101
AccII	CGCG	1 cut(s)	1989
AccIII	TCCGGA	1 cut(s)	348
AciI	CCGC	6 cut(s)	75, 921, 1062, 1268, 1989, 2086
AclWI	GGATC	5 cut(s)	1120, 1543, 1555, 1834, 2138
AcoI	YGGCCR	7 cut(s)	182, 907, 1258, 1495, 1522, 1970, 2053
AcsI	RAATTY	7 cut(s)	467, 557, 788, 901, 1571, 1711, 2110
AcuI	CTGAAG	2 cut(s)	179, 1254
AcyI	GRCGYC	1 cut(s)	1963
AfaI	GTAC	6 cut(s)	887, 1415, 1469, 1821, 1862, 2075
AfeI	AGCGCT	1 cut(s)	1627
AfiI	CCNNNNNNNGG	6 cut(s)	266, 520, 756, 1857, 1972, 2091
AflIII	ACRYGT	3 cut(s)	15, 36, 882
AgeI	ACCGGT	1 cut(s)	565
AgsI	TTSAA	6 cut(s)	551, 1006, 1243, 1814, 2098, 2129
AjnI	CCWGG	3 cut(s)	265, 589, 1885
AjuI	GAANNNNNNNTTGG	6 cut(s)	26, 58, 366, 398, 543, 575
Alw21I	GWGCWC	3 cut(s)	589, 1659, 1882
Alw26I	GTCTC	4 cut(s)	546, 638, 1388, 1658
Alw44I	GTGCAC	3 cut(s)	585, 1655, 1878
AlwI	GGATC	5 cut(s)	1120, 1543, 1555, 1834, 2138
AlwNI	CAGNNNCTG	1 cut(s)	1115
Aor13HI	TCCGGA	1 cut(s)	348
Aor51HI	AGCGCT	1 cut(s)	1627
ApaLI	GTGCAC	3 cut(s)	585, 1655, 1878
ApeKI	GCWGC	3 cut(s)	455, 614, 1124
ApoI	RAATTY	7 cut(s)	467, 557, 788, 901, 1571, 1711, 2110
AseI	ATTAAT	1 cut(s)	1944
AsiGI	ACCGGT	1 cut(s)	565
Asp700I	GAANNNNTTC	1 cut(s)	1804
Asp718I	GGTACC	1 cut(s)	2073
AspLEI	GCGC	1 cut(s)	1628
AspS9I	GGNCC	9 cut(s)	24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
AsuC2I	CCSGG	1 cut(s)	417
AsuHPI	GGTGA	2 cut(s)	1781, 2153
AvaII	GGWCC	6 cut(s)	527, 563, 923, 1203, 1436, 1746
BaeGI	GKGCMC	3 cut(s)	589, 1659, 1882
BalI	TGGCCA	4 cut(s)	1260, 1497, 1524, 2055
BanI	GGYRCC	1 cut(s)	2073
BanII	GRGCYC	1 cut(s)	1268
BauI	CACGAG	1 cut(s)	2089
BbsI	GAAGAC	2 cut(s)	63, 232
Bbv12I	GWGCWC	3 cut(s)	589, 1659, 1882
BbvI	GCAGC	3 cut(s)	467, 626, 1111
BccI	CCATC	4 cut(s)	203, 1031, 1731, 2064
BceAI	ACGGC	1 cut(s)	1168
BcgI	CGANNNNNNTGC	2 cut(s)	1307, 1341
BciT130I	CCWGG	3 cut(s)	267, 591, 1887
BcnI	CCSGG	1 cut(s)	417
BcoDI	GTCTC	4 cut(s)	546, 638, 1388, 1658
BfaI	CTAG	4 cut(s)	810, 939, 962, 1389
BfmI	CTRYAG	2 cut(s)	1398, 1640
BfoI	RGCGCY	1 cut(s)	1629
BfuAI	ACCTGC	2 cut(s)	823, 1200
BisI	GCNGC	4 cut(s)	456, 615, 1062, 1125
BlpI	GCTNAGC	1 cut(s)	1035
BlsI	GCNGC	4 cut(s)	457, 616, 1063, 1126
Bme1390I	CCNGG	4 cut(s)	267, 417, 591, 1887
Bme18I	GGWCC	6 cut(s)	527, 563, 923, 1203, 1436, 1746
BmgT120I	GGNCC	9 cut(s)	24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
BmiI	GGNNCC	5 cut(s)	648, 925, 987, 1748, 2075
BmrFI	CCNGG	4 cut(s)	267, 417, 591, 1887
BmrI	ACTGGG	1 cut(s)	651
BmsI	GCATC	5 cut(s)	664, 943, 1048, 1384, 1965
BmuI	ACTGGG	1 cut(s)	651
BoxI	GACNNNNGTC	2 cut(s)	236, 441
BpiI	GAAGAC	2 cut(s)	63, 232
BpmI	CTGGAG	1 cut(s)	1698
Bpu1102I	GCTNAGC	1 cut(s)	1035
BpuMI	CCSGG	1 cut(s)	417
Bsa29I	ATCGAT	1 cut(s)	693
BsaHI	GRCGYC	1 cut(s)	1963
BsaI	GGTCTC	1 cut(s)	638
BsaJI	CCNNGG	6 cut(s)	27, 265, 650, 889, 980, 1199
BsaWI	WCCGGW	2 cut(s)	348, 565
Bsc4I	CCNNNNNNNGG	6 cut(s)	266, 520, 756, 1857, 1972, 2091
Bse118I	RCCGGY	3 cut(s)	565, 1690, 1972
Bse1I	ACTGG	5 cut(s)	646, 778, 1105, 1286, 1465
Bse3DI	GCAATG	4 cut(s)	465, 709, 930, 1748
BseAI	TCCGGA	1 cut(s)	348
BseBI	CCWGG	3 cut(s)	267, 591, 1887
BseCI	ATCGAT	1 cut(s)	693
BseDI	CCNNGG	6 cut(s)	27, 265, 650, 889, 980, 1199
BseGI	GGATG	2 cut(s)	226, 1399
BseLI	CCNNNNNNNGG	6 cut(s)	266, 520, 756, 1857, 1972, 2091
BseMI	GCAATG	4 cut(s)	465, 709, 930, 1748
BseMII	CTCAG	7 cut(s)	99, 165, 533, 557, 1026, 1583, 2078
BseNI	ACTGG	5 cut(s)	646, 778, 1105, 1286, 1465
BseSI	GKGCMC	3 cut(s)	589, 1659, 1882
BseXI	GCAGC	3 cut(s)	467, 626, 1111
BsgI	GTGCAG	2 cut(s)	801, 998
Bsh1236I	CGCG	1 cut(s)	1989
BshNI	GGYRCC	1 cut(s)	2073
BshTI	ACCGGT	1 cut(s)	565
BshVI	ATCGAT	1 cut(s)	693
BsiHKAI	GWGCWC	3 cut(s)	589, 1659, 1882
BsiSI	CCGG	6 cut(s)	185, 349, 417, 566, 1691, 1973
BslFI	GGGAC	2 cut(s)	909, 1543
BslI	CCNNNNNNNGG	6 cut(s)	266, 520, 756, 1857, 1972, 2091
BsmAI	GTCTC	4 cut(s)	546, 638, 1388, 1658
BsmFI	GGGAC	2 cut(s)	909, 1543
BsmI	GAATGC	3 cut(s)	1127, 1212, 1678
Bso31I	GGTCTC	1 cut(s)	638
Bsp1286I	GDGCHC	4 cut(s)	589, 1268, 1659, 1882
Bsp13I	TCCGGA	1 cut(s)	348
Bsp143I	GATC	6 cut(s)	295, 1112, 1535, 1547, 1826, 2143
Bsp1720I	GCTNAGC	1 cut(s)	1035
Bsp19I	CCATGG	2 cut(s)	650, 889
BspACI	CCGC	6 cut(s)	75, 921, 1062, 1268, 1989, 2086
BspCNI	CTCAG	7 cut(s)	98, 166, 532, 556, 1027, 1584, 2077
BspDI	ATCGAT	1 cut(s)	693
BspEI	TCCGGA	1 cut(s)	348
BspFNI	CGCG	1 cut(s)	1989
BspLI	GGNNCC	5 cut(s)	648, 925, 987, 1748, 2075
BspMAI	CTGCAG	1 cut(s)	1644
BspMI	ACCTGC	2 cut(s)	823, 1200
BspPI	GGATC	5 cut(s)	1120, 1543, 1555, 1834, 2138
BspT107I	GGYRCC	1 cut(s)	2073
BspTNI	GGTCTC	1 cut(s)	638
BsrBI	CCGCTC	1 cut(s)	2088
BsrDI	GCAATG	4 cut(s)	465, 709, 930, 1748
BsrFI	RCCGGY	3 cut(s)	565, 1690, 1972
BsrI	ACTGG	5 cut(s)	646, 778, 1105, 1286, 1465
BssAI	RCCGGY	3 cut(s)	565, 1690, 1972
BssECI	CCNNGG	6 cut(s)	27, 265, 650, 889, 980, 1199
BssMI	GATC	6 cut(s)	295, 1112, 1535, 1547, 1826, 2143
BssNAI	GTATAC	2 cut(s)	783, 1102
BssNI	GRCGYC	1 cut(s)	1963
BssSI	CACGAG	1 cut(s)	2089
BssT1I	CCWWGG	5 cut(s)	27, 650, 889, 980, 1199
Bst1107I	GTATAC	2 cut(s)	783, 1102
Bst2BI	CACGAG	1 cut(s)	2089
Bst2UI	CCWGG	3 cut(s)	267, 591, 1887
Bst4CI	ACNGT	2 cut(s)	51, 716
BstACI	GRCGYC	1 cut(s)	1963
BstAPI	GCANNNNNTGC	1 cut(s)	1133
BstDEI	CTNAG	9 cut(s)	85, 174, 519, 543, 1035, 1592, 1622, 2064, 2151
BstDSI	CCRYGG	2 cut(s)	650, 889
BstF5I	GGATG	2 cut(s)	226, 1399
BstFNI	CGCG	1 cut(s)	1989
BstH2I	RGCGCY	1 cut(s)	1629
BstHHI	GCGC	1 cut(s)	1628
BstKTI	GATC	6 cut(s)	298, 1115, 1538, 1550, 1829, 2146
BstMAI	GTCTC	4 cut(s)	546, 638, 1388, 1658
BstMBI	GATC	6 cut(s)	295, 1112, 1535, 1547, 1826, 2143
BstNI	CCWGG	3 cut(s)	267, 591, 1887
BstNSI	RCATGY	4 cut(s)	19, 40, 886, 1511
BstPAI	GACNNNNGTC	2 cut(s)	236, 441
BstSCI	CCNGG	4 cut(s)	265, 415, 589, 1885
BstSFI	CTRYAG	2 cut(s)	1398, 1640
BstSLI	GKGCMC	3 cut(s)	589, 1659, 1882
BstUI	CGCG	1 cut(s)	1989
BstV1I	GCAGC	3 cut(s)	467, 626, 1111
BstV2I	GAAGAC	2 cut(s)	63, 232
BstX2I	RGATCY	2 cut(s)	1112, 1535
BstXI	CCANNNNNNTGG	2 cut(s)	1532, 1757
BstYI	RGATCY	2 cut(s)	1112, 1535
BstZ17I	GTATAC	2 cut(s)	783, 1102
Bsu15I	ATCGAT	1 cut(s)	693
BsuTUI	ATCGAT	1 cut(s)	693
BtgI	CCRYGG	2 cut(s)	650, 889
BtsCI	GGATG	2 cut(s)	226, 1399
BtsI	GCAGTG	2 cut(s)	1071, 1245
BtsIMutI	CAGTG	4 cut(s)	93, 639, 1071, 1245
BveI	ACCTGC	2 cut(s)	823, 1200
CaiI	CAGNNNCTG	1 cut(s)	1115
CfoI	GCGC	1 cut(s)	1628
Cfr10I	RCCGGY	3 cut(s)	565, 1690, 1972
Cfr13I	GGNCC	9 cut(s)	24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
ClaI	ATCGAT	1 cut(s)	693
CseI	GACGC	1 cut(s)	1971
CsiI	ACCWGGT	1 cut(s)	589
Csp6I	GTAC	6 cut(s)	886, 1414, 1468, 1820, 1861, 2074
CspAI	ACCGGT	1 cut(s)	565
CviQI	GTAC	6 cut(s)	886, 1414, 1468, 1820, 1861, 2074
DdeI	CTNAG	9 cut(s)	85, 174, 519, 543, 1035, 1592, 1622, 2064, 2151
DpnI	GATC	6 cut(s)	297, 1114, 1537, 1549, 1828, 2145
DpnII	GATC	6 cut(s)	295, 1112, 1535, 1547, 1826, 2143
EaeI	YGGCCR	7 cut(s)	182, 907, 1258, 1495, 1522, 1970, 2053
Eco130I	CCWWGG	5 cut(s)	27, 650, 889, 980, 1199
Eco24I	GRGCYC	1 cut(s)	1268
Eco31I	GGTCTC	1 cut(s)	638
Eco32I	GATATC	1 cut(s)	1426
Eco47I	GGWCC	6 cut(s)	527, 563, 923, 1203, 1436, 1746
Eco47III	AGCGCT	1 cut(s)	1627
Eco57I	CTGAAG	2 cut(s)	179, 1254
EcoO109I	RGGNCCY	2 cut(s)	527, 1436
EcoRI	GAATTC	1 cut(s)	1711
EcoRII	CCWGG	3 cut(s)	265, 589, 1885
EcoRV	GATATC	1 cut(s)	1426
EcoT14I	CCWWGG	5 cut(s)	27, 650, 889, 980, 1199
EcoT38I	GRGCYC	1 cut(s)	1268
ErhI	CCWWGG	5 cut(s)	27, 650, 889, 980, 1199
FaqI	GGGAC	2 cut(s)	909, 1543
FauI	CCCGC	2 cut(s)	1275, 2093
FblI	GTMKAC	2 cut(s)	782, 1101
Fnu4HI	GCNGC	4 cut(s)	456, 615, 1062, 1125
FokI	GGATG	2 cut(s)	233, 1406
FriOI	GRGCYC	1 cut(s)	1268
Fsp4HI	GCNGC	4 cut(s)	456, 615, 1062, 1125
FspBI	CTAG	4 cut(s)	810, 939, 962, 1389
GlaI	GCGC	1 cut(s)	1627
GluI	GCNGC	4 cut(s)	456, 615, 1062, 1125
GsuI	CTGGAG	1 cut(s)	1698
HaeII	RGCGCY	1 cut(s)	1629
HapII	CCGG	6 cut(s)	185, 349, 417, 566, 1691, 1973
HgaI	GACGC	1 cut(s)	1971
HhaI	GCGC	1 cut(s)	1628
Hin1I	GRCGYC	1 cut(s)	1963
Hin6I	GCGC	1 cut(s)	1626
HinP1I	GCGC	1 cut(s)	1626
HindIII	AAGCTT	2 cut(s)	1308, 1610
HinfI	GANTC	4 cut(s)	133, 390, 1487, 1556
HpaII	CCGG	6 cut(s)	185, 349, 417, 566, 1691, 1973
HphI	GGTGA	2 cut(s)	1781, 2153
Hpy166II	GTNNAC	6 cut(s)	587, 759, 783, 1102, 1657, 1880
Hpy188I	TCNGA	8 cut(s)	198, 431, 546, 1364, 1953, 1961, 2067, 2148
Hpy8I	GTNNAC	6 cut(s)	587, 759, 783, 1102, 1657, 1880
HpyAV	CCTTC	5 cut(s)	123, 193, 490, 937, 1339
HpyCH4III	ACNGT	2 cut(s)	51, 716
HpyF3I	CTNAG	9 cut(s)	85, 174, 519, 543, 1035, 1592, 1622, 2064, 2151
Hsp92I	GRCGYC	1 cut(s)	1963
HspAI	GCGC	1 cut(s)	1626
Kpn2I	TCCGGA	1 cut(s)	348
KpnI	GGTACC	1 cut(s)	2077
KroI	GCCGGC	1 cut(s)	1972
KroNI	GCCGGC	1 cut(s)	1974
Kzo9I	GATC	6 cut(s)	295, 1112, 1535, 1547, 1826, 2143
Lsp1109I	GCAGC	3 cut(s)	467, 626, 1111
LweI	GCATC	5 cut(s)	664, 943, 1048, 1384, 1965
MabI	ACCWGGT	1 cut(s)	589
MaeI	CTAG	4 cut(s)	810, 939, 962, 1389
MaeIII	GTNAC	3 cut(s)	1165, 1693, 1731
MalI	GATC	6 cut(s)	297, 1114, 1537, 1549, 1828, 2145
MbiI	CCGCTC	1 cut(s)	2088
MboI	GATC	6 cut(s)	295, 1112, 1535, 1547, 1826, 2143
MflI	RGATCY	2 cut(s)	1112, 1535
MhlI	GDGCHC	4 cut(s)	589, 1268, 1659, 1882
MlsI	TGGCCA	4 cut(s)	1260, 1497, 1524, 2055
MluNI	TGGCCA	4 cut(s)	1260, 1497, 1524, 2055
MlyI	GAGTC	1 cut(s)	1565
MmeI	TCCRAC	2 cut(s)	1342, 2171
MnlI	CCTC	9 cut(s)	94, 206, 277, 380, 518, 969, 1084, 1793, 2071
Mox20I	TGGCCA	4 cut(s)	1260, 1497, 1524, 2055
MroI	TCCGGA	1 cut(s)	348
MroNI	GCCGGC	1 cut(s)	1972
MroXI	GAANNNNTTC	1 cut(s)	1804
MscI	TGGCCA	4 cut(s)	1260, 1497, 1524, 2055
MseI	TTAA	6 cut(s)	20, 1454, 1575, 1605, 1874, 1944
Msp20I	TGGCCA	4 cut(s)	1260, 1497, 1524, 2055
MspA1I	CMGCKG	1 cut(s)	1493
MspI	CCGG	6 cut(s)	185, 349, 417, 566, 1691, 1973
MspR9I	CCNGG	4 cut(s)	267, 417, 591, 1887
Mva1269I	GAATGC	3 cut(s)	1127, 1212, 1678
MvaI	CCWGG	3 cut(s)	267, 591, 1887
MvnI	CGCG	1 cut(s)	1989
NaeI	GCCGGC	1 cut(s)	1974
NciI	CCSGG	1 cut(s)	417
NcoI	CCATGG	2 cut(s)	650, 889
NdeII	GATC	6 cut(s)	295, 1112, 1535, 1547, 1826, 2143
NgoMIV	GCCGGC	1 cut(s)	1972
NlaIV	GGNNCC	5 cut(s)	648, 925, 987, 1748, 2075
NmuCI	GTSAC	3 cut(s)	1165, 1693, 1731
NspI	RCATGY	4 cut(s)	19, 40, 886, 1511
PciI	ACATGT	3 cut(s)	15, 36, 882
PctI	GAATGC	3 cut(s)	1127, 1212, 1678
PdiI	GCCGGC	1 cut(s)	1974
PdmI	GAANNNNTTC	1 cut(s)	1804
PfeI	GAWTC	3 cut(s)	133, 390, 1487
PflMI	CCANNNNNTGG	1 cut(s)	756
PfoI	TCCNGGA	1 cut(s)	415
PinAI	ACCGGT	1 cut(s)	565
PkrI	GCNGC	4 cut(s)	457, 616, 1063, 1126
PleI	GAGTC	1 cut(s)	1564
PpsI	GAGTC	1 cut(s)	1564
PpuMI	RGGWCCY	2 cut(s)	527, 1436
PscI	ACATGT	3 cut(s)	15, 36, 882
PshAI	GACNNNNGTC	2 cut(s)	236, 441
PshBI	ATTAAT	1 cut(s)	1944
Psp5II	RGGWCCY	2 cut(s)	527, 1436
Psp6I	CCWGG	3 cut(s)	265, 589, 1885
PspGI	CCWGG	3 cut(s)	265, 589, 1885
PspN4I	GGNNCC	5 cut(s)	648, 925, 987, 1748, 2075
PspPI	GGNCC	9 cut(s)	24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
PspPPI	RGGWCCY	2 cut(s)	527, 1436
PstI	CTGCAG	1 cut(s)	1644
PstNI	CAGNNNCTG	1 cut(s)	1115
PsuI	RGATCY	2 cut(s)	1112, 1535
PvuII	CAGCTG	1 cut(s)	1493
RsaI	GTAC	6 cut(s)	887, 1415, 1469, 1821, 1862, 2075
RsaNI	GTAC	6 cut(s)	886, 1414, 1468, 1820, 1861, 2074
SaqAI	TTAA	6 cut(s)	20, 1454, 1575, 1605, 1874, 1944
SatI	GCNGC	4 cut(s)	456, 615, 1062, 1125
Sau3AI	GATC	6 cut(s)	295, 1112, 1535, 1547, 1826, 2143
Sau96I	GGNCC	9 cut(s)	24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
SchI	GAGTC	1 cut(s)	1565
ScrFI	CCNGG	4 cut(s)	267, 417, 591, 1887
SduI	GDGCHC	4 cut(s)	589, 1268, 1659, 1882
SexAI	ACCWGGT	1 cut(s)	589
SfaNI	GCATC	5 cut(s)	664, 943, 1048, 1384, 1965
SfcI	CTRYAG	2 cut(s)	1398, 1640
SinI	GGWCC	6 cut(s)	527, 563, 923, 1203, 1436, 1746
SsiI	CCGC	6 cut(s)	75, 921, 1062, 1268, 1989, 2086
SspI	AATATT	1 cut(s)	275
SspMI	CTAG	4 cut(s)	810, 939, 962, 1389
StyD4I	CCNGG	4 cut(s)	265, 415, 589, 1885
StyI	CCWWGG	5 cut(s)	27, 650, 889, 980, 1199
TaaI	ACNGT	2 cut(s)	51, 716
TaqI	TCGA	4 cut(s)	693, 1317, 1485, 1846
TauI	GCSGC	1 cut(s)	1064
TfiI	GAWTC	3 cut(s)	133, 390, 1487
Tru1I	TTAA	6 cut(s)	20, 1454, 1575, 1605, 1874, 1944
Tru9I	TTAA	6 cut(s)	20, 1454, 1575, 1605, 1874, 1944
TscAI	CASTG	4 cut(s)	93, 646, 1071, 1252
TseFI	GTSAC	3 cut(s)	1165, 1693, 1731
TseI	GCWGC	3 cut(s)	455, 614, 1124
Tsp45I	GTSAC	3 cut(s)	1165, 1693, 1731
TspGWI	ACGGA	1 cut(s)	1147
TspRI	CASTG	4 cut(s)	93, 646, 1071, 1252
Van91I	CCANNNNNTGG	1 cut(s)	756
VneI	GTGCAC	3 cut(s)	585, 1655, 1878
VpaK11BI	GGWCC	6 cut(s)	527, 563, 923, 1203, 1436, 1746
VspI	ATTAAT	1 cut(s)	1944
XapI	RAATTY	7 cut(s)	467, 557, 788, 901, 1571, 1711, 2110
XbaI	TCTAGA	1 cut(s)	961
XceI	RCATGY	4 cut(s)	19, 40, 886, 1511
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1112, 1793
XmiI	GTMKAC	2 cut(s)	782, 1101
XmnI	GAANNNNTTC	1 cut(s)	1804
XspI	CTAG	4 cut(s)	810, 939, 962, 1389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.