MD13G1126600.v1.1

beta-galactosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002889
Physical Location & Seq
Reverse (-)
9442538 .. 9445280
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1126600.v1.1.491

Sequence Viewer

Length: 387 bp
ATGCGATCTAAAGTTCTTTGGAAGAGAGGATACGATCAAACGTTTATTGGTAATTGCAGCAGAAGTTGTTACTCGAATTTTCCAGTTTTTCTCTGTGGCTTTCCATACTCTTTATCATCAAGTATTATTTTGAGGGACGAATATGATTTCGTCAAGTTCATCAAGATGGTGCAACAAGCAAGCCTATTTGTTAATCTCCAGATTGGCCCTTATGTTTGCGCTGAATGGAACTTCGGGGGATTCCCAGTTTGGCTGAAATATGTCTCCGGAATAGTTTTTCGAAGGACAATGAGCCTTTCAAGGGCAGAGAAGCTGTTTCAAACTCAAGGAGGTCCTATAATTCTTTCTCAGGCTAAGAATTGTGGTAGAAGAGTTTATTGTGGTTAG

Protein Analysis

129

Amino Acids

14.71

Weight (kDa)

9.62

Isoelectric Point (pI)

58.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 46 - 120 1.7e-18 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 266
AclI AACGTT 1 cut(s) 41
AcsI RAATTY 1 cut(s) 76
AfiI CCNNNNNNNGG 1 cut(s) 301
AgsI TTSAA 2 cut(s) 300, 320
AluBI AGCT 1 cut(s) 313
AluI AGCT 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 268
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 1 cut(s) 57
ApoI RAATTY 1 cut(s) 76
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 206, 332
AsuII TTCGAA 1 cut(s) 280
AvaII GGWCC 1 cut(s) 332
BarI GAAGNNNNNNTAC 2 cut(s) 14, 46
BbvI GCAGC 1 cut(s) 69
BccI CCATC 1 cut(s) 160
BciVI GTATCC 1 cut(s) 23
BcoDI GTCTC 1 cut(s) 268
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 1 cut(s) 58
BlsI GCNGC 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 206, 332
BmrI ACTGGG 1 cut(s) 239
BmuI ACTGGG 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 309
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse1I ACTGG 2 cut(s) 83, 245
BseAI TCCGGA 1 cut(s) 266
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMII CTCAG 1 cut(s) 362
BseNI ACTGG 2 cut(s) 83, 245
BseXI GCAGC 1 cut(s) 69
BshFI GGCC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 301
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 1 cut(s) 207
Bsp119I TTCGAA 1 cut(s) 280
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 2 cut(s) 5, 34
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 1 cut(s) 361
BspEI TCCGGA 1 cut(s) 266
BspT104I TTCGAA 1 cut(s) 280
BsrI ACTGG 2 cut(s) 83, 245
BssMI GATC 2 cut(s) 5, 34
Bst6I CTCTTC 2 cut(s) 17, 364
BstBI TTCGAA 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 181
BstDEI CTNAG 2 cut(s) 348, 354
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 2 cut(s) 8, 37
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 2 cut(s) 5, 34
BstV1I GCAGC 1 cut(s) 69
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 181
CfoI GCGC 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 206, 332
CviJI RGCY 7 cut(s) 99, 183, 207, 253, 294, 313, 353
CviKI_1 RGCY 7 cut(s) 99, 183, 207, 253, 294, 313, 353
DdeI CTNAG 2 cut(s) 348, 354
DpnI GATC 2 cut(s) 7, 36
DpnII GATC 2 cut(s) 5, 34
Eam1104I CTCTTC 2 cut(s) 17, 364
EarI CTCTTC 2 cut(s) 17, 364
Eco47I GGWCC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 332
FaiI YATR 5 cut(s) 106, 144, 213, 261, 338
FaqI GGGAC 1 cut(s) 149
Fnu4HI GCNGC 1 cut(s) 58
Fsp4HI GCNGC 1 cut(s) 58
GlaI GCGC 1 cut(s) 220
GluI GCNGC 1 cut(s) 58
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 267
HhaI GCGC 1 cut(s) 221
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HinfI GANTC 1 cut(s) 240
HpaII CCGG 1 cut(s) 267
Hpy188III TCNNGA 3 cut(s) 163, 199, 267
HpyAV CCTTC 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 2 cut(s) 57, 172
HpyF3I CTNAG 2 cut(s) 348, 354
HpySE526I ACGT 1 cut(s) 41
HspAI GCGC 1 cut(s) 219
Kpn2I TCCGGA 1 cut(s) 266
Kzo9I GATC 2 cut(s) 5, 34
LpnPI CCDG 5 cut(s) 96, 212, 258, 280, 335
Lsp1109I GCAGC 1 cut(s) 69
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 68
MalI GATC 2 cut(s) 7, 36
MboI GATC 2 cut(s) 5, 34
MboII GAAGA 2 cut(s) 34, 381
MluCI AATT 4 cut(s) 52, 76, 339, 358
MnlI CCTC 3 cut(s) 20, 126, 323
MroI TCCGGA 1 cut(s) 266
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 164
MspI CCGG 1 cut(s) 267
NdeII GATC 2 cut(s) 5, 34
NspV TTCGAA 1 cut(s) 280
PfeI GAWTC 1 cut(s) 240
PkrI GCNGC 1 cut(s) 59
PpuMI RGGWCCY 1 cut(s) 332
Psp1406I AACGTT 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 332
PspPI GGNCC 2 cut(s) 206, 332
PspPPI RGGWCCY 1 cut(s) 332
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 1 cut(s) 58
Sau3AI GATC 2 cut(s) 5, 34
Sau96I GGNCC 2 cut(s) 206, 332
SetI ASST 3 cut(s) 44, 315, 334
SfuI TTCGAA 1 cut(s) 280
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 324
SmoI CTYRAG 1 cut(s) 324
Sse9I AATT 4 cut(s) 52, 76, 339, 358
TaiI ACGT 1 cut(s) 44
TaqI TCGA 2 cut(s) 74, 280
TasI AATT 4 cut(s) 52, 76, 339, 358
TfiI GAWTC 1 cut(s) 240
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 1 cut(s) 57
TspDTI ATGAA 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 332
XapI RAATTY 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.