Rw2G006610

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002889
Physical Location & Seq
Reverse (-)
6367916 .. 6373065
5150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006610.1

Sequence Viewer

Length: 2199 bp
ATGTGGAACTCTAACATGTTAAGGGCCAAGGCCAACATGTTCTTCCTACTGTTTCTGGTTTCATGGCTTTCTTCTGCTTTAGCCTCTGTGGGTTATGACCACAGGGCTGTTATAGTCGATGGAAAGAGAAGGATTCTCCTTTCTGGGTCTATTCACTACCCCAGAAGCACACCGGAGATGTGGCCGGATTTGCTTCAGAAGGCCAAAGATGGAGGCTTGGATGTGATACAGACCTATGTCTTCTGGAATGGGCACGAACCAAGCCCAGGAAAATATTATTTTGAGGATAGATATGATCTGGTCAAGTTCATCAAGCTGGCTCAACAACATGGCCTATACGTTCATCTCCGGATTGGCCCTTATATTTGTGCTGAATGGAACTTTGGAGGATTCCCTGTTTGGCTGAAATATGTTCCCGGAATGGTTTTCAGAACAGACAATCGTCCTTTCATGGCAGCAATGGAAAAATTTACACAGAAGATTGTGTATATGATGAAGGCAGAAAGGTTGTTCCAAACTCAGGGAGGTCCTATAATTCTGTCTCAGATTGAAAATGAATTTGGACCGGTAGAGTGGGAAATTGGTGCACCTGGTAAATCTTATACTCAATGGGCAGCTAAAATGGCTGTGGGTCTCAACACTGGGGTTCCATGGGTTATGTGCAAGCAAGAAGATGCCCCTGACCCAATTATCGATACTTGCAATGGCTTCTACTGTGAAAACTTCACTCCAAACAAGAACTATAAGCCCAAAATGTGGACAGAAATCTGGACTGGCTGGTATACAGAATTTGGTGGGGCAGTTCCTACTAGACCTGCACAGGACTTGGCCTTTTCAGTTGCAAGGTTTATACAAAATGGTGGTTCTTTTGCCAACTACTACATGTACCATGGGGGAACAAATTTTGGCCGAACTGCTGGCGGTCCCTTCATTGCAACTAGCTATGACTATGATGCACCTCTTGATGAATATGGACTACCAAGGGAACCAAAATATAGCCATTTGAAATATCTGCACAAAGCCATCAAATTAGCTGAGCCAGCTTTAGTGGCTACTGATGCCGCAGTGTCAAAACTTGGAAATAATCAAGAGGCTCATGTATACCAGTCAAGATCTGGTTGTGCTGCATTCCTTGCAAATTACGACACAAAATACTCCGTGGGAGTGACATTTTGGAATAAGCCATATAACCTGCCACCTTGGTCCATAAGCATTCTTCCTGATTGCAAAACTGAAGTTTTCAACACTGCTAGGGTTGGCCAGAGCCCGCCAACGAAGATGACACCAGTAAGTCACCTTTCTTGGCAAGCTTACATCGAAGAAGTTGCCGCTTCTACTGATGAGAAGGCATTTACATTGGTCGGACTTCGTGAGCAAATAAGTCTCACTAGGGATGCTACAGACTACTTGTGGTACATGACAGATATCACAATAGGTCCTAATGAACAATTTTTAAGAACCGGAAAGTACCCCACTCTCAAAGTCGATTCAGCTGGCCATGCCTTGCATGTTTTCATCAATGGTCAACTATCAGGATCTGCTTATGGATCATTGGAGTCCCCCAAGTTAGAATTTAATCAGGATGTGAAGCTGAGAGCTGGCATTAACAAGCTTGCTTTACTTAGCACTTCTGTTGGTCTGCCGAATGTTGGTGTGCACTTTGAGACATGGAATGCTGGAGTTCTTGGGCCGGTCACATTGACGGGTGTGAATTCGGGAACATGGGATATGTCACGATGGCAATGGACCCACAAGATTGGTATGAAAGGTGAAGATATGAGCCTCCATACCATTAGTGGAAGTTCTTCTGTTGAATGGGTACAAGGATCACTATTGGCTCAAAATCGACCCCTTGCATGGTACAAGGCTATTTTAAATATGGGTAGCATGGGAAAGGGTCAAATGTGGATTAATGGTCAGAGCATCGGACGCCATTGGCCGGCATATATAGCACGCGGTAGTTGTGGTGCTTGTTATTATGCTGGAACTTATACCGAAAACAAATGCCGAACAAATTGTGGCCAGCCCTCTCAGAGATGGTACCATGTTCCCCGCTCGTGGTTGAAGCCAAGTGGGAATTTATTGGTTGTGTTTGAAGAATGGGGTGGTGATCCGACTAAGATTGCTTTGGTAGCAAGAAACCAGGAACCACATGGTTTTGCTTCAGATCAATGCACATTGCACTACCTTGTGTATTGA

Protein Analysis

732

Amino Acids

82.25

Weight (kDa)

8.5

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 341 1.7e-120 Glycosyl hydrolases family 35
GHD PF17834 364 - 419 8.7e-23 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 469 - 522 1.6e-06 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 623 - 693 2.3e-18 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 823, 1200
Acc65I GGTACC 1 cut(s) 2040
AccB1I GGYRCC 1 cut(s) 2040
AccB7I CCANNNNNTGG 1 cut(s) 756
AccBSI CCGCTC 1 cut(s) 2055
AccI GTMKAC 2 cut(s) 782, 1101
AccII CGCG 1 cut(s) 1956
AccIII TCCGGA 1 cut(s) 348
AciI CCGC 6 cut(s) 921, 1062, 1268, 1329, 1956, 2053
AclWI GGATC 4 cut(s) 1543, 1555, 1834, 2105
AcoI YGGCCR 6 cut(s) 182, 907, 1258, 1495, 1937, 2020
AcsI RAATTY 7 cut(s) 467, 557, 788, 901, 1571, 1711, 2077
AcuI CTGAAG 3 cut(s) 179, 1254, 2148
AcyI GRCGYC 1 cut(s) 1930
AfaI GTAC 6 cut(s) 887, 1415, 1469, 1821, 1862, 2042
AfiI CCNNNNNNNGG 7 cut(s) 266, 520, 756, 1649, 1857, 1939, 2058
AflIII ACRYGT 3 cut(s) 15, 36, 882
AgeI ACCGGT 1 cut(s) 565
AgsI TTSAA 6 cut(s) 551, 1006, 1243, 1814, 2065, 2096
AjnI CCWGG 3 cut(s) 265, 589, 2142
AjuI GAANNNNNNNTTGG 6 cut(s) 26, 58, 366, 398, 543, 575
Alw21I GWGCWC 2 cut(s) 589, 1659
Alw26I GTCTC 4 cut(s) 546, 638, 1388, 1658
Alw44I GTGCAC 2 cut(s) 585, 1655
AlwI GGATC 4 cut(s) 1543, 1555, 1834, 2105
AlwNI CAGNNNCTG 1 cut(s) 1538
Aor13HI TCCGGA 1 cut(s) 348
ApaLI GTGCAC 2 cut(s) 585, 1655
ApeKI GCWGC 3 cut(s) 455, 614, 1124
ApoI RAATTY 7 cut(s) 467, 557, 788, 901, 1571, 1711, 2077
AseI ATTAAT 1 cut(s) 1911
AsiGI ACCGGT 1 cut(s) 565
Asp700I GAANNNNTTC 1 cut(s) 1804
Asp718I GGTACC 1 cut(s) 2040
AspS9I GGNCC 9 cut(s) 24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 3 cut(s) 1286, 1781, 2120
AvaII GGWCC 6 cut(s) 527, 563, 923, 1203, 1436, 1746
BaeGI GKGCMC 3 cut(s) 255, 589, 1659
BalI TGGCCA 3 cut(s) 1260, 1497, 2022
BanI GGYRCC 1 cut(s) 2040
BanII GRGCYC 1 cut(s) 1268
BauI CACGAG 1 cut(s) 2056
BbsI GAAGAC 1 cut(s) 232
Bbv12I GWGCWC 2 cut(s) 589, 1659
BbvI GCAGC 3 cut(s) 467, 626, 1111
BccI CCATC 5 cut(s) 113, 203, 1031, 1731, 2031
BcgI CGANNNNNNTGC 2 cut(s) 1307, 1341
BciT130I CCWGG 3 cut(s) 267, 591, 2144
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 4 cut(s) 546, 638, 1388, 1658
BfaI CTAG 4 cut(s) 810, 939, 1251, 1389
BfmI CTRYAG 1 cut(s) 1398
BfuAI ACCTGC 2 cut(s) 823, 1200
BglII AGATCT 1 cut(s) 1112
BisI GCNGC 5 cut(s) 456, 615, 1062, 1125, 1329
BlpI GCTNAGC 1 cut(s) 1035
BlsI GCNGC 5 cut(s) 457, 616, 1063, 1126, 1330
Bme1390I CCNGG 4 cut(s) 267, 417, 591, 2144
Bme18I GGWCC 6 cut(s) 527, 563, 923, 1203, 1436, 1746
BmgT120I GGNCC 9 cut(s) 24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
BmiI GGNNCC 6 cut(s) 648, 925, 987, 1748, 2042, 2148
BmrFI CCNGG 4 cut(s) 267, 417, 591, 2144
BmrI ACTGGG 1 cut(s) 651
BmsI GCATC 5 cut(s) 664, 943, 1048, 1384, 1932
BmuI ACTGGG 1 cut(s) 651
BoxI GACNNNNGTC 2 cut(s) 236, 441
BpiI GAAGAC 1 cut(s) 232
BpmI CTGGAG 1 cut(s) 1698
Bpu1102I GCTNAGC 1 cut(s) 1035
BpuMI CCSGG 1 cut(s) 417
Bsa29I ATCGAT 1 cut(s) 693
BsaHI GRCGYC 1 cut(s) 1930
BsaI GGTCTC 1 cut(s) 638
BsaJI CCNNGG 7 cut(s) 27, 265, 650, 889, 980, 1158, 1199
BsaWI WCCGGW 4 cut(s) 172, 348, 565, 1460
Bsc4I CCNNNNNNNGG 7 cut(s) 266, 520, 756, 1649, 1857, 1939, 2058
Bse118I RCCGGY 3 cut(s) 565, 1690, 1939
Bse1I ACTGG 4 cut(s) 646, 778, 1105, 1286
Bse3DI GCAATG 5 cut(s) 465, 709, 930, 1748, 2177
BseAI TCCGGA 1 cut(s) 348
BseBI CCWGG 3 cut(s) 267, 591, 2144
BseCI ATCGAT 1 cut(s) 693
BseDI CCNNGG 7 cut(s) 27, 265, 650, 889, 980, 1158, 1199
BseGI GGATG 3 cut(s) 226, 1399, 1588
BseLI CCNNNNNNNGG 7 cut(s) 266, 520, 756, 1649, 1857, 1939, 2058
BseMI GCAATG 5 cut(s) 465, 709, 930, 1748, 2177
BseMII CTCAG 5 cut(s) 533, 557, 1026, 1583, 2045
BseNI ACTGG 4 cut(s) 646, 778, 1105, 1286
BseSI GKGCMC 3 cut(s) 255, 589, 1659
BseXI GCAGC 3 cut(s) 467, 626, 1111
BsgI GTGCAG 2 cut(s) 801, 998
Bsh1236I CGCG 1 cut(s) 1956
BshNI GGYRCC 1 cut(s) 2040
BshTI ACCGGT 1 cut(s) 565
BshVI ATCGAT 1 cut(s) 693
BsiHKAI GWGCWC 2 cut(s) 589, 1659
BsiSI CCGG 8 cut(s) 173, 185, 349, 417, 566, 1461, 1691, 1940
BslFI GGGAC 2 cut(s) 909, 1543
BslI CCNNNNNNNGG 7 cut(s) 266, 520, 756, 1649, 1857, 1939, 2058
BsmAI GTCTC 4 cut(s) 546, 638, 1388, 1658
BsmFI GGGAC 2 cut(s) 909, 1543
BsmI GAATGC 3 cut(s) 1127, 1212, 1678
Bso31I GGTCTC 1 cut(s) 638
Bsp1286I GDGCHC 4 cut(s) 255, 589, 1268, 1659
Bsp13I TCCGGA 1 cut(s) 348
Bsp143I GATC 7 cut(s) 295, 1112, 1535, 1547, 1826, 2110, 2167
Bsp1720I GCTNAGC 1 cut(s) 1035
Bsp19I CCATGG 2 cut(s) 650, 889
BspACI CCGC 6 cut(s) 921, 1062, 1268, 1329, 1956, 2053
BspCNI CTCAG 5 cut(s) 532, 556, 1027, 1584, 2044
BspDI ATCGAT 1 cut(s) 693
BspEI TCCGGA 1 cut(s) 348
BspFNI CGCG 1 cut(s) 1956
BspLI GGNNCC 6 cut(s) 648, 925, 987, 1748, 2042, 2148
BspMI ACCTGC 2 cut(s) 823, 1200
BspPI GGATC 4 cut(s) 1543, 1555, 1834, 2105
BspT107I GGYRCC 1 cut(s) 2040
BspTNI GGTCTC 1 cut(s) 638
BsrBI CCGCTC 1 cut(s) 2055
BsrDI GCAATG 5 cut(s) 465, 709, 930, 1748, 2177
BsrFI RCCGGY 3 cut(s) 565, 1690, 1939
BsrI ACTGG 4 cut(s) 646, 778, 1105, 1286
BssAI RCCGGY 3 cut(s) 565, 1690, 1939
BssECI CCNNGG 7 cut(s) 27, 265, 650, 889, 980, 1158, 1199
BssMI GATC 7 cut(s) 295, 1112, 1535, 1547, 1826, 2110, 2167
BssNAI GTATAC 2 cut(s) 783, 1102
BssNI GRCGYC 1 cut(s) 1930
BssSI CACGAG 1 cut(s) 2056
BssT1I CCWWGG 5 cut(s) 27, 650, 889, 980, 1199
Bst1107I GTATAC 2 cut(s) 783, 1102
Bst2BI CACGAG 1 cut(s) 2056
Bst2UI CCWGG 3 cut(s) 267, 591, 2144
Bst4CI ACNGT 2 cut(s) 51, 716
BstACI GRCGYC 1 cut(s) 1930
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstDEI CTNAG 7 cut(s) 519, 543, 1035, 1592, 1622, 2031, 2118
BstDSI CCRYGG 3 cut(s) 650, 889, 1158
BstF5I GGATG 3 cut(s) 226, 1399, 1588
BstFNI CGCG 1 cut(s) 1956
BstKTI GATC 7 cut(s) 298, 1115, 1538, 1550, 1829, 2113, 2170
BstMAI GTCTC 4 cut(s) 546, 638, 1388, 1658
BstMBI GATC 7 cut(s) 295, 1112, 1535, 1547, 1826, 2110, 2167
BstNI CCWGG 3 cut(s) 267, 591, 2144
BstNSI RCATGY 4 cut(s) 19, 40, 886, 1511
BstPAI GACNNNNGTC 2 cut(s) 236, 441
BstSCI CCNGG 4 cut(s) 265, 415, 589, 2142
BstSFI CTRYAG 1 cut(s) 1398
BstSLI GKGCMC 3 cut(s) 255, 589, 1659
BstUI CGCG 1 cut(s) 1956
BstV1I GCAGC 3 cut(s) 467, 626, 1111
BstV2I GAAGAC 1 cut(s) 232
BstX2I RGATCY 2 cut(s) 1112, 1535
BstXI CCANNNNNNTGG 1 cut(s) 1757
BstYI RGATCY 2 cut(s) 1112, 1535
BstZ17I GTATAC 2 cut(s) 783, 1102
Bsu15I ATCGAT 1 cut(s) 693
BsuTUI ATCGAT 1 cut(s) 693
BtgI CCRYGG 3 cut(s) 650, 889, 1158
BtsCI GGATG 3 cut(s) 226, 1399, 1588
BtsI GCAGTG 2 cut(s) 1071, 1245
BtsIMutI CAGTG 3 cut(s) 639, 1071, 1245
BveI ACCTGC 2 cut(s) 823, 1200
CaiI CAGNNNCTG 1 cut(s) 1538
Cfr10I RCCGGY 3 cut(s) 565, 1690, 1939
Cfr13I GGNCC 9 cut(s) 24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
ClaI ATCGAT 1 cut(s) 693
CseI GACGC 1 cut(s) 1938
CsiI ACCWGGT 1 cut(s) 589
Csp6I GTAC 6 cut(s) 886, 1414, 1468, 1820, 1861, 2041
CspAI ACCGGT 1 cut(s) 565
CviQI GTAC 6 cut(s) 886, 1414, 1468, 1820, 1861, 2041
DdeI CTNAG 7 cut(s) 519, 543, 1035, 1592, 1622, 2031, 2118
DpnI GATC 7 cut(s) 297, 1114, 1537, 1549, 1828, 2112, 2169
DpnII GATC 7 cut(s) 295, 1112, 1535, 1547, 1826, 2110, 2167
DraI TTTAAA 1 cut(s) 1875
EaeI YGGCCR 6 cut(s) 182, 907, 1258, 1495, 1937, 2020
Eco130I CCWWGG 5 cut(s) 27, 650, 889, 980, 1199
Eco24I GRGCYC 1 cut(s) 1268
Eco31I GGTCTC 1 cut(s) 638
Eco32I GATATC 1 cut(s) 1426
Eco47I GGWCC 6 cut(s) 527, 563, 923, 1203, 1436, 1746
Eco57I CTGAAG 3 cut(s) 179, 1254, 2148
EcoO109I RGGNCCY 2 cut(s) 527, 1436
EcoRI GAATTC 1 cut(s) 1711
EcoRII CCWGG 3 cut(s) 265, 589, 2142
EcoRV GATATC 1 cut(s) 1426
EcoT14I CCWWGG 5 cut(s) 27, 650, 889, 980, 1199
EcoT38I GRGCYC 1 cut(s) 1268
ErhI CCWWGG 5 cut(s) 27, 650, 889, 980, 1199
FaqI GGGAC 2 cut(s) 909, 1543
FauI CCCGC 2 cut(s) 1275, 2060
FblI GTMKAC 2 cut(s) 782, 1101
Fnu4HI GCNGC 5 cut(s) 456, 615, 1062, 1125, 1329
FokI GGATG 3 cut(s) 233, 1406, 1595
FriOI GRGCYC 1 cut(s) 1268
Fsp4HI GCNGC 5 cut(s) 456, 615, 1062, 1125, 1329
FspBI CTAG 4 cut(s) 810, 939, 1251, 1389
GluI GCNGC 5 cut(s) 456, 615, 1062, 1125, 1329
GsuI CTGGAG 1 cut(s) 1698
HapII CCGG 8 cut(s) 173, 185, 349, 417, 566, 1461, 1691, 1940
HgaI GACGC 1 cut(s) 1938
Hin1I GRCGYC 1 cut(s) 1930
HincII GTYRAC 1 cut(s) 1526
HindII GTYRAC 1 cut(s) 1526
HindIII AAGCTT 2 cut(s) 1308, 1610
HinfI GANTC 4 cut(s) 133, 390, 1487, 1556
HpaII CCGG 8 cut(s) 173, 185, 349, 417, 566, 1461, 1691, 1940
HphI GGTGA 3 cut(s) 1286, 1781, 2120
Hpy166II GTNNAC 6 cut(s) 587, 759, 783, 1102, 1526, 1657
Hpy188I TCNGA 9 cut(s) 198, 431, 546, 1364, 1920, 1928, 2034, 2115, 2167
Hpy8I GTNNAC 6 cut(s) 587, 759, 783, 1102, 1526, 1657
HpyAV CCTTC 5 cut(s) 123, 193, 490, 937, 1339
HpyCH4III ACNGT 2 cut(s) 51, 716
HpyCH4IV ACGT 1 cut(s) 339
HpyF3I CTNAG 7 cut(s) 519, 543, 1035, 1592, 1622, 2031, 2118
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 1930
Kpn2I TCCGGA 1 cut(s) 348
KpnI GGTACC 1 cut(s) 2044
KroI GCCGGC 1 cut(s) 1939
KroNI GCCGGC 1 cut(s) 1941
Kzo9I GATC 7 cut(s) 295, 1112, 1535, 1547, 1826, 2110, 2167
Lsp1109I GCAGC 3 cut(s) 467, 626, 1111
LweI GCATC 5 cut(s) 664, 943, 1048, 1384, 1932
MabI ACCWGGT 1 cut(s) 589
MaeI CTAG 4 cut(s) 810, 939, 1251, 1389
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 4 cut(s) 1165, 1292, 1693, 1731
MalI GATC 7 cut(s) 297, 1114, 1537, 1549, 1828, 2112, 2169
MbiI CCGCTC 1 cut(s) 2055
MboI GATC 7 cut(s) 295, 1112, 1535, 1547, 1826, 2110, 2167
MflI RGATCY 2 cut(s) 1112, 1535
MhlI GDGCHC 4 cut(s) 255, 589, 1268, 1659
MlsI TGGCCA 3 cut(s) 1260, 1497, 2022
MluNI TGGCCA 3 cut(s) 1260, 1497, 2022
MlyI GAGTC 1 cut(s) 1565
MmeI TCCRAC 2 cut(s) 1342, 2138
MnlI CCTC 9 cut(s) 94, 206, 277, 380, 518, 969, 1084, 1793, 2038
Mox20I TGGCCA 3 cut(s) 1260, 1497, 2022
MroI TCCGGA 1 cut(s) 348
MroNI GCCGGC 1 cut(s) 1939
MroXI GAANNNNTTC 1 cut(s) 1804
MscI TGGCCA 3 cut(s) 1260, 1497, 2022
MseI TTAA 6 cut(s) 20, 1454, 1575, 1605, 1874, 1911
Msp20I TGGCCA 3 cut(s) 1260, 1497, 2022
MspA1I CMGCKG 1 cut(s) 1493
MspI CCGG 8 cut(s) 173, 185, 349, 417, 566, 1461, 1691, 1940
MspR9I CCNGG 4 cut(s) 267, 417, 591, 2144
Mva1269I GAATGC 3 cut(s) 1127, 1212, 1678
MvaI CCWGG 3 cut(s) 267, 591, 2144
MvnI CGCG 1 cut(s) 1956
NaeI GCCGGC 1 cut(s) 1941
NciI CCSGG 1 cut(s) 417
NcoI CCATGG 2 cut(s) 650, 889
NdeII GATC 7 cut(s) 295, 1112, 1535, 1547, 1826, 2110, 2167
NgoMIV GCCGGC 1 cut(s) 1939
NlaIV GGNNCC 6 cut(s) 648, 925, 987, 1748, 2042, 2148
NmuCI GTSAC 4 cut(s) 1165, 1292, 1693, 1731
NspI RCATGY 4 cut(s) 19, 40, 886, 1511
PciI ACATGT 3 cut(s) 15, 36, 882
PctI GAATGC 3 cut(s) 1127, 1212, 1678
PdiI GCCGGC 1 cut(s) 1941
PdmI GAANNNNTTC 1 cut(s) 1804
PfeI GAWTC 3 cut(s) 133, 390, 1487
PflMI CCANNNNNTGG 1 cut(s) 756
PfoI TCCNGGA 1 cut(s) 415
PinAI ACCGGT 1 cut(s) 565
PkrI GCNGC 5 cut(s) 457, 616, 1063, 1126, 1330
PleI GAGTC 1 cut(s) 1564
PpsI GAGTC 1 cut(s) 1564
PpuMI RGGWCCY 2 cut(s) 527, 1436
PscI ACATGT 3 cut(s) 15, 36, 882
PshAI GACNNNNGTC 2 cut(s) 236, 441
PshBI ATTAAT 1 cut(s) 1911
Psp5II RGGWCCY 2 cut(s) 527, 1436
Psp6I CCWGG 3 cut(s) 265, 589, 2142
PspGI CCWGG 3 cut(s) 265, 589, 2142
PspN4I GGNNCC 6 cut(s) 648, 925, 987, 1748, 2042, 2148
PspPI GGNCC 9 cut(s) 24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
PspPPI RGGWCCY 2 cut(s) 527, 1436
PstNI CAGNNNCTG 1 cut(s) 1538
PsuI RGATCY 2 cut(s) 1112, 1535
PvuII CAGCTG 1 cut(s) 1493
RsaI GTAC 6 cut(s) 887, 1415, 1469, 1821, 1862, 2042
RsaNI GTAC 6 cut(s) 886, 1414, 1468, 1820, 1861, 2041
SaqAI TTAA 6 cut(s) 20, 1454, 1575, 1605, 1874, 1911
SatI GCNGC 5 cut(s) 456, 615, 1062, 1125, 1329
Sau3AI GATC 7 cut(s) 295, 1112, 1535, 1547, 1826, 2110, 2167
Sau96I GGNCC 9 cut(s) 24, 356, 527, 563, 923, 1203, 1436, 1688, 1746
SchI GAGTC 1 cut(s) 1565
ScrFI CCNGG 4 cut(s) 267, 417, 591, 2144
SduI GDGCHC 4 cut(s) 255, 589, 1268, 1659
SexAI ACCWGGT 1 cut(s) 589
SfaNI GCATC 5 cut(s) 664, 943, 1048, 1384, 1932
SfcI CTRYAG 1 cut(s) 1398
SinI GGWCC 6 cut(s) 527, 563, 923, 1203, 1436, 1746
SsiI CCGC 6 cut(s) 921, 1062, 1268, 1329, 1956, 2053
SspI AATATT 1 cut(s) 275
SspMI CTAG 4 cut(s) 810, 939, 1251, 1389
StyD4I CCNGG 4 cut(s) 265, 415, 589, 2142
StyI CCWWGG 5 cut(s) 27, 650, 889, 980, 1199
TaaI ACNGT 2 cut(s) 51, 716
TaiI ACGT 1 cut(s) 342
TaqI TCGA 5 cut(s) 117, 693, 1317, 1485, 1846
TauI GCSGC 2 cut(s) 1064, 1331
TfiI GAWTC 3 cut(s) 133, 390, 1487
Tru1I TTAA 6 cut(s) 20, 1454, 1575, 1605, 1874, 1911
Tru9I TTAA 6 cut(s) 20, 1454, 1575, 1605, 1874, 1911
TscAI CASTG 3 cut(s) 646, 1071, 1252
TseFI GTSAC 4 cut(s) 1165, 1292, 1693, 1731
TseI GCWGC 3 cut(s) 455, 614, 1124
Tsp45I GTSAC 4 cut(s) 1165, 1292, 1693, 1731
TspGWI ACGGA 1 cut(s) 1147
TspRI CASTG 3 cut(s) 646, 1071, 1252
Van91I CCANNNNNTGG 1 cut(s) 756
VneI GTGCAC 2 cut(s) 585, 1655
VpaK11BI GGWCC 6 cut(s) 527, 563, 923, 1203, 1436, 1746
VspI ATTAAT 1 cut(s) 1911
XapI RAATTY 7 cut(s) 467, 557, 788, 901, 1571, 1711, 2077
XceI RCATGY 4 cut(s) 19, 40, 886, 1511
XcmI CCANNNNNNNNNTGG 3 cut(s) 1112, 1793, 2150
XmiI GTMKAC 2 cut(s) 782, 1101
XmnI GAANNNNTTC 1 cut(s) 1804
XspI CTAG 4 cut(s) 810, 939, 1251, 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.