Rw3G008200

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002889
Physical Location & Seq
Forward (+)
7609164 .. 7613034
3871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G008200.1

Sequence Viewer

Length: 2106 bp
ATGATTCTCATTTCAGGGTCTATTCACTACCCCAGAAGCACACATGAGATGTGGCCGGATTTGATTCAGAAGGCCAAAGATGGAGGCTTGGATCCCAGGAAAATATTATTTTGTGATCCAGACTTATATGATTTGGTCAAGTTCATCAAGCTGGCTCAACTACATGGCCTATATGTTCATCTCCGCATTGGTCCTTATATTTGTGCTGAATGGAACTTTGAAAGATTCCCTGTTTGGCTGAAATATGTTCCCGGAATTGCTTTCAGAACAGACAATCGTCCTTTCATGGCAGAAATGGAAAAATTTACACAGAAGATTGTGTATATGATGAAGGCAGAAAGGTTGTTCCAAACTCAGGGAGAGTGGGAAATTGGTGAACCTGGTAAATCTTATACTCAATGGGCAGCGAAAATGGCTGTGGCTCTCAACACTGGGGTTCCATGGGTTATGTGCAAGCAAGAAGATTCCCCTGATCCAATCATCGACACTTGCAATGGCTTCTGCTGTGAAAACTTCACTCCAAACAAGAACTATAAGGCCAAAATGTGGACAGAAATCTGGACTGGCTGGTATACAGAATTTGGTGGGGCGGTTCCTACTAGACCTGCACAGGACTTGGCATTTTCAGTTGCAAGGTTTATACAAAACGGTGGTTCTTTTGCCAATTACTACATGTACCATGGAGGAACAAATTTTGGCAGAACTGCTGGCTGTCCCTTCATCGCAACTAGCTATGACTATGATGCCCCTCTAGATGAATATGGACTACCAAGGGAACCAAAATATAGCCATTTGAAATATCTGCACAGAGCCATCAAATTGGCTGAGTCAGCTTTAGTGGCTACTGATGCTGCAGTGTCAAAACTCGGAAATAATCAAGAGGCTCATGTATACCAGTCGATATCTGGTTGTGCTGCATTCCTTGCAAATTACGACACAAAATACTCCGTGAGAGTGATGTTTTGGAATAAGCAATATAACCTGCCACCTTGGTCCATAAGCATTCTCCCTGACTGCAAAACTGAAGTTTTCAACACTGCAAGGATTGGCCAGAGCCCGCCGACGAAGATGACACCAGTAGCATCCCTTTCTTGGCAGGGGTACGTGGAAGAAATTGCCACTGCTAATGATGACAATGCAATTACATTGGTCGGACTTCGTGAGCAAATAAGTCAAACTAGGGATGCTACAGACTACTTGTGGTACACGACCAATATCACAATAGGTCCTAATGAACAATTTCTAAGAACCGGACAGTACCCTATTCTCACTGTCGATTCAGCTGGGCATGCCTTGCTTGTTTTCATCAATGAATTCAAGCAGGGTGTGAAGCTGAGAGCTAGCATTAACAAGCTTGCTTTACTAAGCGTTTCTGTTGGTCTACCGAATGTTGGTGTGCACTTTGAGACATGGAATGCCGGAGTTCTAGGGCCAGTCACATTGAAGGGTGTGAATTCGGGAACATGGGATATGTCACGATGGCAATGGACCTATAAGACTGGTATAAAAGGTGAAGATATGAGCCTCCATACCGTGGGTGGAAGTTCTTCTATTAAATGGGTACAAGGATCACTATTGGCTCAAAATCAACCCCTTACATGGTACAAGGCTATTTTAGATGCACCACCAGGAAACGCACCATTAGCTTTAGATATGGGTAGCATGGGAAAGGGTCAAATGTGGATTAATGGTCGGAGCATCGGACGCCATTGGCCAGCATATACAGCAAAAGGCACCTGTGGTACTTGTTATTATGCTGGAACTTATACTGAAAACAAATGCCGAACAAATTGTGGACAGCCTTCCCAGAGATGGTACCATGTTCCCCGCTCGTGGTTGAAGCCAAGTGGGAATTTATTGGTTGTGTTTGAAGAATGGGGTGGTGATCCGACTAAGATTGCTTTGGTAGCAAGAAGGATAAGACGTCCCTGTTTCTCATACTCATTTGTCCAATTTCTGTTAATTCCCAATCTGTATATTCTTCATGCATGTAAAATTGTTATAGATGTCTACAATTTCACTTACATTTTACATTTTGCTTGTGCCTCAAGGTACCATAATTTCTTGATGTCAGCATTGCTAAAGAATGAAATATCCCATCACTAG

Protein Analysis

701

Amino Acids

79.25

Weight (kDa)

8.71

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 2 - 31 5.7e-07 Glycosyl hydrolases family 35
Glyco_hydro_35 PF01301 43 - 271 1.1e-66 Glycosyl hydrolases family 35
GHD PF17834 294 - 349 2.4e-22 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 532 - 618 5.1e-24 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1927
Acc36I ACCTGC 2 cut(s) 613, 990
Acc65I GGTACC 2 cut(s) 1815, 2052
AccB1I GGYRCC 3 cut(s) 1733, 1815, 2052
AccB7I CCANNNNNTGG 2 cut(s) 546, 1534
AccBSI CCGCTC 1 cut(s) 1830
AccI GTMKAC 4 cut(s) 572, 891, 1381, 2010
AciI CCGC 4 cut(s) 184, 590, 1058, 1828
AclWI GGATC 6 cut(s) 86, 99, 110, 467, 1576, 1880
AcoI YGGCCR 3 cut(s) 53, 1048, 1712
AcsI RAATTY 6 cut(s) 302, 578, 691, 1313, 1453, 1852
AcuI CTGAAG 1 cut(s) 1044
AcyI GRCGYC 2 cut(s) 1705, 1924
AfaI GTAC 9 cut(s) 677, 1103, 1205, 1259, 1563, 1604, 1744, 1817, 2054
AfiI CCNNNNNNNGG 8 cut(s) 355, 546, 1092, 1391, 1534, 1599, 1812, 1833
AflIII ACRYGT 1 cut(s) 672
AgsI TTSAA 7 cut(s) 221, 796, 1033, 1318, 1444, 1840, 1871
AjnI CCWGG 3 cut(s) 95, 379, 1627
AluBI AGCT 8 cut(s) 151, 732, 833, 1283, 1333, 1340, 1354, 1646
AluI AGCT 8 cut(s) 151, 732, 833, 1283, 1333, 1340, 1354, 1646
Alw21I GWGCWC 1 cut(s) 1401
Alw26I GTCTC 1 cut(s) 1400
Alw44I GTGCAC 1 cut(s) 1397
AlwI GGATC 6 cut(s) 86, 99, 110, 467, 1576, 1880
AoxI GGCC 7 cut(s) 53, 72, 166, 537, 1048, 1430, 1712
ApaLI GTGCAC 1 cut(s) 1397
ApeKI GCWGC 3 cut(s) 404, 851, 914
ApoI RAATTY 6 cut(s) 302, 578, 691, 1313, 1453, 1852
AseI ATTAAT 1 cut(s) 1686
Asp700I GAANNNNTTC 2 cut(s) 1239, 1546
Asp718I GGTACC 2 cut(s) 1815, 2052
AspS9I GGNCC 5 cut(s) 191, 993, 1226, 1430, 1488
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 3 cut(s) 386, 1523, 1895
AsuNHI GCTAGC 1 cut(s) 1340
AvaII GGWCC 4 cut(s) 191, 993, 1226, 1488
BaeGI GKGCMC 1 cut(s) 1401
BalI TGGCCA 2 cut(s) 1050, 1714
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 3 cut(s) 1733, 1815, 2052
BanII GRGCYC 1 cut(s) 1058
BauI CACGAG 1 cut(s) 1831
Bbv12I GWGCWC 1 cut(s) 1401
BbvI GCAGC 3 cut(s) 416, 838, 901
BccI CCATC 4 cut(s) 74, 821, 1473, 1806
BciT130I CCWGG 3 cut(s) 97, 381, 1629
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 1400
BfaI CTAG 7 cut(s) 600, 729, 752, 1179, 1341, 1427, 2104
BfmI CTRYAG 2 cut(s) 852, 1188
BfuAI ACCTGC 2 cut(s) 613, 990
BisI GCNGC 3 cut(s) 405, 852, 915
BlsI GCNGC 3 cut(s) 406, 853, 916
Bme1390I CCNGG 4 cut(s) 97, 252, 381, 1629
Bme18I GGWCC 4 cut(s) 191, 993, 1226, 1488
BmgT120I GGNCC 5 cut(s) 191, 993, 1226, 1430, 1488
BmiI GGNNCC 7 cut(s) 93, 438, 594, 777, 1735, 1817, 2054
BmrFI CCNGG 4 cut(s) 97, 252, 381, 1629
BmrI ACTGGG 1 cut(s) 441
BmsI GCATC 6 cut(s) 733, 838, 1091, 1174, 1609, 1707
BmtI GCTAGC 1 cut(s) 1344
BmuI ACTGGG 1 cut(s) 441
BoxI GACNNNNGTC 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 2032
BpuMI CCSGG 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 1105
BsaHI GRCGYC 2 cut(s) 1705, 1924
BsaJI CCNNGG 6 cut(s) 95, 440, 679, 770, 989, 1533
BsaWI WCCGGW 1 cut(s) 1250
Bsc4I CCNNNNNNNGG 8 cut(s) 355, 546, 1092, 1391, 1534, 1599, 1812, 1833
Bse1I ACTGG 6 cut(s) 436, 568, 895, 1076, 1433, 1504
Bse3DI GCAATG 3 cut(s) 499, 1490, 2075
BseBI CCWGG 3 cut(s) 97, 381, 1629
BseDI CCNNGG 6 cut(s) 95, 440, 679, 770, 989, 1533
BseGI GGATG 2 cut(s) 1082, 1189
BseLI CCNNNNNNNGG 8 cut(s) 355, 546, 1092, 1391, 1534, 1599, 1812, 1833
BseMI GCAATG 3 cut(s) 499, 1490, 2075
BseMII CTCAG 3 cut(s) 368, 816, 1325
BseNI ACTGG 6 cut(s) 436, 568, 895, 1076, 1433, 1504
BseSI GKGCMC 1 cut(s) 1401
BseXI GCAGC 3 cut(s) 416, 838, 901
BseYI CCCAGC 1 cut(s) 1283
BsgI GTGCAG 2 cut(s) 591, 788
BshFI GGCC 7 cut(s) 55, 74, 168, 539, 1050, 1432, 1714
BshNI GGYRCC 3 cut(s) 1733, 1815, 2052
BsiHKAI GWGCWC 1 cut(s) 1401
BsiSI CCGG 4 cut(s) 56, 252, 1251, 1419
BslFI GGGAC 2 cut(s) 699, 1911
BslI CCNNNNNNNGG 8 cut(s) 355, 546, 1092, 1391, 1534, 1599, 1812, 1833
BsmAI GTCTC 1 cut(s) 1400
BsmFI GGGAC 2 cut(s) 699, 1911
BsmI GAATGC 3 cut(s) 917, 1002, 1420
BsnI GGCC 7 cut(s) 55, 74, 168, 539, 1050, 1432, 1714
Bsp1286I GDGCHC 2 cut(s) 1058, 1401
Bsp143I GATC 5 cut(s) 91, 115, 472, 1568, 1885
Bsp19I CCATGG 2 cut(s) 440, 679
BspACI CCGC 4 cut(s) 184, 590, 1058, 1828
BspANI GGCC 7 cut(s) 55, 74, 168, 539, 1050, 1432, 1714
BspCNI CTCAG 3 cut(s) 367, 817, 1326
BspLI GGNNCC 7 cut(s) 93, 438, 594, 777, 1735, 1817, 2054
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 2 cut(s) 613, 990
BspOI GCTAGC 1 cut(s) 1344
BspPI GGATC 6 cut(s) 86, 99, 110, 467, 1576, 1880
BspT107I GGYRCC 3 cut(s) 1733, 1815, 2052
BsrBI CCGCTC 1 cut(s) 1830
BsrDI GCAATG 3 cut(s) 499, 1490, 2075
BsrI ACTGG 6 cut(s) 436, 568, 895, 1076, 1433, 1504
BssECI CCNNGG 6 cut(s) 95, 440, 679, 770, 989, 1533
BssMI GATC 5 cut(s) 91, 115, 472, 1568, 1885
BssNAI GTATAC 2 cut(s) 573, 892
BssNI GRCGYC 2 cut(s) 1705, 1924
BssSI CACGAG 1 cut(s) 1831
BssT1I CCWWGG 4 cut(s) 440, 679, 770, 989
Bst1107I GTATAC 2 cut(s) 573, 892
Bst2BI CACGAG 1 cut(s) 1831
Bst2UI CCWGG 3 cut(s) 97, 381, 1629
Bst4CI ACNGT 4 cut(s) 650, 1257, 1273, 1534
BstACI GRCGYC 2 cut(s) 1705, 1924
BstAPI GCANNNNNTGC 2 cut(s) 923, 1294
BstBAI YACGTR 1 cut(s) 1105
BstC8I GCNNGC 8 cut(s) 153, 455, 709, 1058, 1290, 1342, 1356, 1716
BstDEI CTNAG 6 cut(s) 354, 825, 1244, 1334, 1364, 1893
BstDSI CCRYGG 3 cut(s) 440, 679, 1533
BstF5I GGATG 2 cut(s) 1082, 1189
BstKTI GATC 5 cut(s) 94, 118, 475, 1571, 1888
BstMAI GTCTC 1 cut(s) 1400
BstMBI GATC 5 cut(s) 91, 115, 472, 1568, 1885
BstNI CCWGG 3 cut(s) 97, 381, 1629
BstNSI RCATGY 3 cut(s) 676, 1292, 1992
BstPAI GACNNNNGTC 1 cut(s) 276
BstSCI CCNGG 4 cut(s) 95, 250, 379, 1627
BstSFI CTRYAG 2 cut(s) 852, 1188
BstSLI GKGCMC 1 cut(s) 1401
BstV1I GCAGC 3 cut(s) 416, 838, 901
BstX2I RGATCY 1 cut(s) 91
BstXI CCANNNNNNTGG 1 cut(s) 820
BstYI RGATCY 1 cut(s) 91
BstZ17I GTATAC 2 cut(s) 573, 892
BsuRI GGCC 7 cut(s) 55, 74, 168, 539, 1050, 1432, 1714
BtgI CCRYGG 3 cut(s) 440, 679, 1533
BtgZI GCGATG 1 cut(s) 706
BtsCI GGATG 2 cut(s) 1082, 1189
BtsI GCAGTG 3 cut(s) 861, 1035, 1119
BtsIMutI CAGTG 5 cut(s) 429, 861, 1035, 1119, 1269
BveI ACCTGC 2 cut(s) 613, 990
Cac8I GCNNGC 8 cut(s) 153, 455, 709, 1058, 1290, 1342, 1356, 1716
Cfr13I GGNCC 5 cut(s) 191, 993, 1226, 1430, 1488
CseI GACGC 1 cut(s) 1713
CsiI ACCWGGT 1 cut(s) 379
Csp6I GTAC 9 cut(s) 676, 1102, 1204, 1258, 1562, 1603, 1743, 1816, 2053
CviQI GTAC 9 cut(s) 676, 1102, 1204, 1258, 1562, 1603, 1743, 1816, 2053
DdeI CTNAG 6 cut(s) 354, 825, 1244, 1334, 1364, 1893
DpnI GATC 5 cut(s) 93, 117, 474, 1570, 1887
DpnII GATC 5 cut(s) 91, 115, 472, 1568, 1885
EaeI YGGCCR 3 cut(s) 53, 1048, 1712
Eco130I CCWWGG 4 cut(s) 440, 679, 770, 989
Eco24I GRGCYC 1 cut(s) 1058
Eco32I GATATC 1 cut(s) 903
Eco47I GGWCC 4 cut(s) 191, 993, 1226, 1488
Eco57I CTGAAG 1 cut(s) 1044
EcoO109I RGGNCCY 1 cut(s) 1226
EcoRI GAATTC 2 cut(s) 1313, 1453
EcoRII CCWGG 3 cut(s) 95, 379, 1627
EcoRV GATATC 1 cut(s) 903
EcoT14I CCWWGG 4 cut(s) 440, 679, 770, 989
EcoT22I ATGCAT 1 cut(s) 1990
EcoT38I GRGCYC 1 cut(s) 1058
ErhI CCWWGG 4 cut(s) 440, 679, 770, 989
FaqI GGGAC 2 cut(s) 699, 1911
FauI CCCGC 2 cut(s) 1065, 1835
FblI GTMKAC 4 cut(s) 572, 891, 1381, 2010
Fnu4HI GCNGC 3 cut(s) 405, 852, 915
FokI GGATG 2 cut(s) 1069, 1196
FriOI GRGCYC 1 cut(s) 1058
Fsp4HI GCNGC 3 cut(s) 405, 852, 915
FspBI CTAG 7 cut(s) 600, 729, 752, 1179, 1341, 1427, 2104
GluI GCNGC 3 cut(s) 405, 852, 915
GsaI CCCAGC 1 cut(s) 1287
HaeIII GGCC 7 cut(s) 55, 74, 168, 539, 1050, 1432, 1714
HapII CCGG 4 cut(s) 56, 252, 1251, 1419
HgaI GACGC 1 cut(s) 1713
Hin1I GRCGYC 2 cut(s) 1705, 1924
HindIII AAGCTT 1 cut(s) 1352
HinfI GANTC 6 cut(s) 4, 64, 225, 464, 827, 1277
HpaII CCGG 4 cut(s) 56, 252, 1251, 1419
HphI GGTGA 3 cut(s) 386, 1523, 1895
Hpy166II GTNNAC 9 cut(s) 377, 549, 573, 892, 1206, 1382, 1399, 1796, 2011
Hpy188I TCNGA 7 cut(s) 69, 266, 869, 1154, 1695, 1703, 1890
Hpy188III TCNNGA 8 cut(s) 119, 559, 752, 878, 1160, 1458, 1476, 2065
Hpy8I GTNNAC 9 cut(s) 377, 549, 573, 892, 1206, 1382, 1399, 1796, 2011
Hpy99I CGWCG 1 cut(s) 1066
HpyAV CCTTC 6 cut(s) 64, 325, 727, 1438, 1812, 1908
HpyCH4III ACNGT 4 cut(s) 650, 1257, 1273, 1534
HpyCH4IV ACGT 2 cut(s) 1104, 1924
HpyF3I CTNAG 6 cut(s) 354, 825, 1244, 1334, 1364, 1893
HpySE526I ACGT 2 cut(s) 1104, 1924
Hsp92I GRCGYC 2 cut(s) 1705, 1924
KpnI GGTACC 2 cut(s) 1819, 2056
Kzo9I GATC 5 cut(s) 91, 115, 472, 1568, 1885
LmnI GCTCC 1 cut(s) 1695
Lsp1109I GCAGC 3 cut(s) 416, 838, 901
LweI GCATC 6 cut(s) 733, 838, 1091, 1174, 1609, 1707
MabI ACCWGGT 1 cut(s) 379
MaeI CTAG 7 cut(s) 600, 729, 752, 1179, 1341, 1427, 2104
MaeII ACGT 2 cut(s) 1104, 1924
MaeIII GTNAC 2 cut(s) 1435, 1473
MalI GATC 5 cut(s) 93, 117, 474, 1570, 1887
MbiI CCGCTC 1 cut(s) 1830
MboI GATC 5 cut(s) 91, 115, 472, 1568, 1885
MboII GAAGA 8 cut(s) 325, 473, 1078, 1121, 1526, 1539, 1883, 1973
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 2 cut(s) 1058, 1401
MlsI TGGCCA 2 cut(s) 1050, 1714
MluNI TGGCCA 2 cut(s) 1050, 1714
MlyI GAGTC 1 cut(s) 836
MmeI TCCRAC 3 cut(s) 1132, 1673, 1913
MnlI CCTC 6 cut(s) 77, 677, 759, 874, 1535, 2056
Mox20I TGGCCA 2 cut(s) 1050, 1714
Mph1103I ATGCAT 1 cut(s) 1990
MroXI GAANNNNTTC 2 cut(s) 1239, 1546
MscI TGGCCA 2 cut(s) 1050, 1714
MseI TTAA 4 cut(s) 1347, 1554, 1686, 1961
Msp20I TGGCCA 2 cut(s) 1050, 1714
MspA1I CMGCKG 1 cut(s) 1283
MspI CCGG 4 cut(s) 56, 252, 1251, 1419
MspR9I CCNGG 4 cut(s) 97, 252, 381, 1629
Mva1269I GAATGC 3 cut(s) 917, 1002, 1420
MvaI CCWGG 3 cut(s) 97, 381, 1629
NciI CCSGG 1 cut(s) 252
NcoI CCATGG 2 cut(s) 440, 679
NdeII GATC 5 cut(s) 91, 115, 472, 1568, 1885
NheI GCTAGC 1 cut(s) 1340
NlaIV GGNNCC 7 cut(s) 93, 438, 594, 777, 1735, 1817, 2054
NmuCI GTSAC 2 cut(s) 1435, 1473
NsiI ATGCAT 1 cut(s) 1990
NspI RCATGY 3 cut(s) 676, 1292, 1992
PaeI GCATGC 1 cut(s) 1292
PciI ACATGT 1 cut(s) 672
PctI GAATGC 3 cut(s) 917, 1002, 1420
PdmI GAANNNNTTC 2 cut(s) 1239, 1546
PfeI GAWTC 5 cut(s) 4, 64, 225, 464, 1277
PflMI CCANNNNNTGG 2 cut(s) 546, 1534
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 3 cut(s) 406, 853, 916
PleI GAGTC 1 cut(s) 835
PpsI GAGTC 1 cut(s) 835
Ppu21I YACGTR 1 cut(s) 1105
PpuMI RGGWCCY 1 cut(s) 1226
PscI ACATGT 1 cut(s) 672
PshAI GACNNNNGTC 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 1686
Psp5II RGGWCCY 1 cut(s) 1226
Psp6I CCWGG 3 cut(s) 95, 379, 1627
PspFI CCCAGC 1 cut(s) 1283
PspGI CCWGG 3 cut(s) 95, 379, 1627
PspN4I GGNNCC 7 cut(s) 93, 438, 594, 777, 1735, 1817, 2054
PspPI GGNCC 5 cut(s) 191, 993, 1226, 1430, 1488
PspPPI RGGWCCY 1 cut(s) 1226
PstI CTGCAG 1 cut(s) 856
PsuI RGATCY 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 1283
RsaI GTAC 9 cut(s) 677, 1103, 1205, 1259, 1563, 1604, 1744, 1817, 2054
RsaNI GTAC 9 cut(s) 676, 1102, 1204, 1258, 1562, 1603, 1743, 1816, 2053
SaqAI TTAA 4 cut(s) 1347, 1554, 1686, 1961
SatI GCNGC 3 cut(s) 405, 852, 915
Sau3AI GATC 5 cut(s) 91, 115, 472, 1568, 1885
Sau96I GGNCC 5 cut(s) 191, 993, 1226, 1430, 1488
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 4 cut(s) 97, 252, 381, 1629
SduI GDGCHC 2 cut(s) 1058, 1401
SexAI ACCWGGT 1 cut(s) 379
SfaNI GCATC 6 cut(s) 733, 838, 1091, 1174, 1609, 1707
SfcI CTRYAG 2 cut(s) 852, 1188
SinI GGWCC 4 cut(s) 191, 993, 1226, 1488
SmlI CTYRAG 1 cut(s) 2047
SmoI CTYRAG 1 cut(s) 2047
SphI GCATGC 1 cut(s) 1292
SsiI CCGC 4 cut(s) 184, 590, 1058, 1828
SspI AATATT 1 cut(s) 105
SspMI CTAG 7 cut(s) 600, 729, 752, 1179, 1341, 1427, 2104
StyD4I CCNGG 4 cut(s) 95, 250, 379, 1627
StyI CCWWGG 4 cut(s) 440, 679, 770, 989
TaaI ACNGT 4 cut(s) 650, 1257, 1273, 1534
TaiI ACGT 2 cut(s) 1107, 1927
TaqI TCGA 3 cut(s) 483, 899, 1275
TfiI GAWTC 5 cut(s) 4, 64, 225, 464, 1277
Tru1I TTAA 4 cut(s) 1347, 1554, 1686, 1961
Tru9I TTAA 4 cut(s) 1347, 1554, 1686, 1961
TscAI CASTG 5 cut(s) 436, 861, 1042, 1126, 1276
TseFI GTSAC 2 cut(s) 1435, 1473
TseI GCWGC 3 cut(s) 404, 851, 914
Tsp45I GTSAC 2 cut(s) 1435, 1473
TspGWI ACGGA 1 cut(s) 937
TspRI CASTG 5 cut(s) 436, 861, 1042, 1126, 1276
Van91I CCANNNNNTGG 2 cut(s) 546, 1534
VneI GTGCAC 1 cut(s) 1397
VpaK11BI GGWCC 4 cut(s) 191, 993, 1226, 1488
VspI ATTAAT 1 cut(s) 1686
XapI RAATTY 6 cut(s) 302, 578, 691, 1313, 1453, 1852
XbaI TCTAGA 1 cut(s) 751
XceI RCATGY 3 cut(s) 676, 1292, 1992
XcmI CCANNNNNNNNNTGG 2 cut(s) 902, 1535
XmiI GTMKAC 4 cut(s) 572, 891, 1381, 2010
XmnI GAANNNNTTC 2 cut(s) 1239, 1546
XspI CTAG 7 cut(s) 600, 729, 752, 1179, 1341, 1427, 2104
ZraI GACGTC 1 cut(s) 1925
Zsp2I ATGCAT 1 cut(s) 1990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.