MD08G1139000.v1.1

beta-galactosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002889
Physical Location & Seq
Reverse (-)
13405250 .. 13406649
1400 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1139000.v1.1.491

Sequence Viewer

Length: 339 bp
ATGCGATCTAAAGTTCTTTGGAAGAGAGGATACGATCAAACGTTTACTGGTGATTGCAGCAGAAGTTGTTACTCAAATTTTCCAGTTTTTCTCTGCAGCTTTCCAAACTCTTTATCATCAAGTATTATTTTGAGGGACGAATATGATTTGGTCAAGTTCATCAAGATGGTGCAACAAGCAAGCCTATTTGTTAATCTCCAGATTGGCCCTTATGTTTGTGCTGAATGGAACTTCGGGGGATTCCCAGTTTGGCTGAAATATGTCTCCGGAATCGTTTTTCGAAGGACAATGAGCCTTTCAAGGCAGAGAAGCTGTTTCAAACTCAAGGAGGTCCTATAA

Protein Analysis

113

Amino Acids

13.06

Weight (kDa)

9.54

Isoelectric Point (pI)

64.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 46 - 96 7.7e-18 Glycosyl hydrolases family 35
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 266
AclI AACGTT 1 cut(s) 41
AcsI RAATTY 1 cut(s) 76
AgsI TTSAA 2 cut(s) 300, 319
AluBI AGCT 2 cut(s) 99, 312
AluI AGCT 2 cut(s) 99, 312
Alw26I GTCTC 1 cut(s) 268
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 2 cut(s) 57, 96
ApoI RAATTY 1 cut(s) 76
AspS9I GGNCC 2 cut(s) 206, 331
AsuHPI GGTGA 1 cut(s) 62
AsuII TTCGAA 1 cut(s) 280
AvaII GGWCC 1 cut(s) 331
BarI GAAGNNNNNNTAC 2 cut(s) 14, 46
BbvI GCAGC 2 cut(s) 69, 108
BccI CCATC 1 cut(s) 160
BciVI GTATCC 1 cut(s) 23
BcoDI GTCTC 1 cut(s) 268
BfmI CTRYAG 1 cut(s) 94
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 2 cut(s) 58, 97
BlsI GCNGC 2 cut(s) 59, 98
Bme18I GGWCC 1 cut(s) 331
BmgT120I GGNCC 2 cut(s) 206, 331
BmrI ACTGGG 1 cut(s) 239
BmuI ACTGGG 1 cut(s) 239
BpmI CTGGAG 1 cut(s) 182
Bpu14I TTCGAA 1 cut(s) 280
BpuEI CTTGAG 1 cut(s) 308
BsaWI WCCGGW 1 cut(s) 266
Bse1I ACTGG 3 cut(s) 52, 83, 245
BseAI TCCGGA 1 cut(s) 266
BseNI ACTGG 3 cut(s) 52, 83, 245
BseXI GCAGC 2 cut(s) 69, 108
BshFI GGCC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 149
BsmAI GTCTC 1 cut(s) 268
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 1 cut(s) 207
Bsp119I TTCGAA 1 cut(s) 280
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 2 cut(s) 5, 34
BspANI GGCC 1 cut(s) 207
BspEI TCCGGA 1 cut(s) 266
BspMAI CTGCAG 1 cut(s) 98
BspT104I TTCGAA 1 cut(s) 280
BsrI ACTGG 3 cut(s) 52, 83, 245
BssMI GATC 2 cut(s) 5, 34
Bst6I CTCTTC 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 181
BstKTI GATC 2 cut(s) 8, 37
BstMAI GTCTC 1 cut(s) 268
BstMBI GATC 2 cut(s) 5, 34
BstSFI CTRYAG 1 cut(s) 94
BstV1I GCAGC 2 cut(s) 69, 108
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 1 cut(s) 207
Cac8I GCNNGC 1 cut(s) 181
Cfr13I GGNCC 2 cut(s) 206, 331
CviJI RGCY 6 cut(s) 99, 183, 207, 253, 294, 312
CviKI_1 RGCY 6 cut(s) 99, 183, 207, 253, 294, 312
DpnI GATC 2 cut(s) 7, 36
DpnII GATC 2 cut(s) 5, 34
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 331
FaiI YATR 4 cut(s) 144, 213, 261, 337
FaqI GGGAC 1 cut(s) 149
Fnu4HI GCNGC 2 cut(s) 58, 97
Fsp4HI GCNGC 2 cut(s) 58, 97
GluI GCNGC 2 cut(s) 58, 97
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 267
HinfI GANTC 2 cut(s) 240, 270
HpaII CCGG 1 cut(s) 267
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 1 cut(s) 45
Hpy188III TCNNGA 3 cut(s) 163, 199, 267
Hpy8I GTNNAC 1 cut(s) 45
HpyAV CCTTC 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 3 cut(s) 57, 96, 172
HpySE526I ACGT 1 cut(s) 41
Kpn2I TCCGGA 1 cut(s) 266
Kzo9I GATC 2 cut(s) 5, 34
LpnPI CCDG 5 cut(s) 33, 96, 212, 258, 280
Lsp1109I GCAGC 2 cut(s) 69, 108
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 68
MalI GATC 2 cut(s) 7, 36
MboI GATC 2 cut(s) 5, 34
MboII GAAGA 1 cut(s) 34
MluCI AATT 1 cut(s) 76
MnlI CCTC 3 cut(s) 20, 126, 322
MroI TCCGGA 1 cut(s) 266
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 164
MspI CCGG 1 cut(s) 267
NdeII GATC 2 cut(s) 5, 34
NspV TTCGAA 1 cut(s) 280
PfeI GAWTC 2 cut(s) 240, 270
PkrI GCNGC 2 cut(s) 59, 98
PpuMI RGGWCCY 1 cut(s) 331
Psp1406I AACGTT 1 cut(s) 41
Psp5II RGGWCCY 1 cut(s) 331
PspPI GGNCC 2 cut(s) 206, 331
PspPPI RGGWCCY 1 cut(s) 331
PstI CTGCAG 1 cut(s) 98
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 58, 97
Sau3AI GATC 2 cut(s) 5, 34
Sau96I GGNCC 2 cut(s) 206, 331
SetI ASST 4 cut(s) 44, 101, 314, 333
SfcI CTRYAG 1 cut(s) 94
SfuI TTCGAA 1 cut(s) 280
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
Sse9I AATT 1 cut(s) 76
TaiI ACGT 1 cut(s) 44
TaqI TCGA 1 cut(s) 280
TasI AATT 1 cut(s) 76
TfiI GAWTC 2 cut(s) 240, 270
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseI GCWGC 2 cut(s) 57, 96
TspDTI ATGAA 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 331
XapI RAATTY 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.