RchiOBHm_Chr2g0093311

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002889
Physical Location & Seq
Forward (+)
6617875 .. 6623811
5937 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46838

Sequence Viewer

Length: 2286 bp
ATGGCGAGCCCTGTGAGAACTACGGCGGAAAAAGTGCAGTCAATGTGGAACGGTAACAAGTTGAGCGCCAATGCCAACATGTTCCTGCTATTGTTTCTGTTTTCGTGGCTGTCTTCTGCTACAGCCTCTGTGAGTTATGACCACAGAGCTATAATAATTGATGGACAGAAGAGAATTCTCATTTCTGGTTCCATCCACTATCCCAGAAGCACACCTGAGATGTGGCCTGATTTAATCCAGAAGGCTAAAGATGGAGGCTTGGATGTTATACAAACCTATGTGTTTTGGAACGGGCATGAGCCTTCTCCGGGGAAGTATTATTTTGAGGATAGATATGATTTGGTTAAGTTCATCAAGCTGGTACAAAAAGCAGGCCTGTATGTTCATCTCCGCATTGGTCCTTATGTTTGTGCTGAATGGAACTTTGGGGGATTCCCGGCTTGGCTCAAATATGTTCCGGGAATTGAGTTTAGAACAGACAATGAGCCTTTCAAAGCAGCAATGAAAACATTTACAGAGAAAATTGTCGGTATGATGAAGGCAGAACGACTGTTCCAAACTCAGGGAGGTCCCATTATTCTGTCTCAGATAGAAAATGAGTATGGACCGGTGGAATGGACAATCGGCGCCCCTGGTAAAGCATATACCAATTGGACAGCACAAATGGCTCTTGGGCTTGACACTGGAGTCCCATGGGTAATGTGCAAGCAAGAGGATGCTCCTGACCCAATTATCGATACTTGCAACGGTTTCTACTGTGAAAACTTCACTCCAAACAAGAAGTATAAGCCCAAAATGTGGACCGAAGTGTGGACTGGCTGGTATACAGAATTTGGCGGGGCAGTTCCTACTAGACCTGCACAAGATTTGGCATTTTCAGTTGCAAGGTTTATACAAAATGGTGGCTCTTTTGCTAACTATTACATGTATCATGGAGGAACTAATTTTGACCGAACTTCTGGCCTTTTCGTTGCAACTAGCTATGACTATGATGCACCTCTAGATGAATATGGACTACCAAGGGAACCAAAGTGGAGTCATCTGAAATATTTGCACAAAGCCATCAAATTAGCTGAGGCAGCTTTAGTGGCTACTGATCCTGCAGTGACATCACTTGGAAAGAGTCAAGAGGCTCATGTATTCAATTCAAGTTCTGGGTGTGCTGCATTTCTTGCAAATTACGACACAAAATACTCGTCAAGAGTGAGCTTTGGGAATAGGCAATATAAGCTGCCACCCTGGTCTATAAGCATTCTTCCTGACTGCAAAACTGAAGTTCTCAACACTGGAAGGGTTGGCCAGAGCCCGCCGGTGGATATGACACCAGTAAATACCACCCTTTCTTGGCAGTCGTACATCGAAGATGCTGCCTCTTCTACTGATGACAATACAGTTACATTGGATGGGCTCTATGAGCAAATAAATATCACTAGGGATGCAACAGACTACTTATGGTACATGACAGACATCACAATAGGTCCTAATGAAGAGTTTCTAAAGAATGGATCTTACCCTCTTCTGACTATCGATTCAGCTGGACATGCCTTGCATGTTTTCATCAATGGTCAACTATCAGGAACTGTTTATGCACGATTGGCATCTCCTAAATTAAGATTTAATGACACTGTGAAGCTCAGAGCTGGCATCAACAAGCTTGCTTTACTAAGCATTGCTGTTGGTCTGCCTAATGTTGGTGTGCACTTTGAGACTTGGAATGCTGGAGTTCTAGGGCCGGTCACATTGAAGGGTCTGAATTCGGGAACATGGGACATGTCCCGGTGGAAATGGACCTACAAGACTGGTATGAAAGGTGAAGCATTAAGCCTCCATACTGTTAAGGGAAGTTCTTCCGTTGAATGGGTAGAAGGACCACTATTGGCTCAAAAACAACCCCTTACATGGTACAAGTCTATATTTCATGCACCACCGGGAAATGCTCCATTAGCTTTAGATATGGCTAGCATGGGCAAAGGTCAAGTATGGATCAATGGTCAGAGCATCGGACGCCACTGGCCTGGATACATAGCACATGGTAGCTGTGGCAATTGTTCTTATGCTGGAACTTACACCGAAAACAAATGCCGAACAAATTGTGGTCAGCCGTCACAGAGATGGTACCATGTTCCGCAATCGTGGTTGAACCAGAGTGAAAATTTGTTGGTGGCGTTTGAAGAATGGGGCGGTGACCCGACTAAGATTTCTCTGGTGGCACGAAGCTATGAAAACTTAGGGCAGACCCTCTATAACATAGAGGGCGGCTCCAACCATTTGAGATTGCAATTGTAG

Protein Analysis

761

Amino Acids

84.88

Weight (kDa)

6.99

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 51 - 354 2.4e-117 Glycosyl hydrolases family 35
GHD PF17834 377 - 432 1e-19 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 483 - 536 3.9e-06 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 632 - 718 1.2e-24 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
Acc36I ACCTGC 1 cut(s) 865
Acc65I GGTACC 1 cut(s) 2115
AccB1I GGYRCC 2 cut(s) 626, 2115
AccB7I CCANNNNNTGG 1 cut(s) 798
AccI GTMKAC 1 cut(s) 824
AciI CCGC 7 cut(s) 26, 391, 837, 1307, 2126, 2181, 2256
AclWI GGATC 3 cut(s) 1091, 1513, 1991
AcoI YGGCCR 1 cut(s) 1297
AcsI RAATTY 4 cut(s) 174, 830, 1753, 2152
AcuI CTGAAG 1 cut(s) 1293
AcyI GRCGYC 2 cut(s) 627, 2005
AfaI GTAC 5 cut(s) 363, 1355, 1457, 1904, 2117
AfiI CCNNNNNNNGG 9 cut(s) 308, 562, 798, 810, 1312, 1344, 1691, 1857, 1899
AflIII ACRYGT 3 cut(s) 78, 924, 1770
AgeI ACCGGT 1 cut(s) 607
AgsI TTSAA 7 cut(s) 493, 1144, 1149, 1744, 1856, 2140, 2171
AjnI CCWGG 3 cut(s) 631, 1238, 2014
AjuI GAANNNNNNNTTGG 2 cut(s) 408, 440
AloI GAACNNNNNNTCC 2 cut(s) 537, 569
Alw21I GWGCWC 1 cut(s) 1701
Alw26I GTCTC 2 cut(s) 588, 1700
Alw44I GTGCAC 1 cut(s) 1697
AlwI GGATC 3 cut(s) 1091, 1513, 1991
AlwNI CAGNNNCTG 2 cut(s) 128, 1580
AoxI GGCC 6 cut(s) 224, 373, 961, 1297, 1730, 2012
ApaLI GTGCAC 1 cut(s) 1697
ApeKI GCWGC 5 cut(s) 497, 1079, 1163, 1231, 1367
ApoI RAATTY 4 cut(s) 174, 830, 1753, 2152
ArsI GACNNNNNNTTYG 2 cut(s) 1181, 1213
AsiGI ACCGGT 1 cut(s) 607
Asp700I GAANNNNTTC 2 cut(s) 1491, 1846
Asp718I GGTACC 1 cut(s) 2115
AspLEI GCGC 2 cut(s) 68, 629
AspS9I GGNCC 8 cut(s) 398, 569, 605, 801, 1478, 1730, 1788, 1868
AsuC2I CCSGG 5 cut(s) 309, 437, 459, 1777, 1929
AsuHPI GGTGA 2 cut(s) 1823, 2195
AsuNHI GCTAGC 1 cut(s) 1958
AvaII GGWCC 7 cut(s) 398, 569, 605, 801, 1478, 1788, 1868
BaeGI GKGCMC 1 cut(s) 1701
BalI TGGCCA 1 cut(s) 1299
BanI GGYRCC 2 cut(s) 626, 2115
BanII GRGCYC 3 cut(s) 11, 1307, 1410
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 1701
BbvCI CCTCAGC 1 cut(s) 1074
BbvI GCAGC 5 cut(s) 509, 1091, 1150, 1218, 1354
BccI CCATC 6 cut(s) 155, 200, 245, 1070, 1397, 2106
BceAI ACGGC 2 cut(s) 39, 2086
BcgI CGANNNNNNTGC 2 cut(s) 1349, 1383
BciT130I CCWGG 3 cut(s) 633, 1240, 2016
BciVI GTATCC 1 cut(s) 2012
BcnI CCSGG 5 cut(s) 309, 437, 459, 1777, 1929
BcoDI GTCTC 2 cut(s) 588, 1700
BfaI CTAG 6 cut(s) 852, 978, 1001, 1431, 1727, 1959
BfmI CTRYAG 2 cut(s) 120, 1101
BfoI RGCGCY 2 cut(s) 69, 630
BfuAI ACCTGC 1 cut(s) 865
BfuI GTATCC 1 cut(s) 2012
BisI GCNGC 6 cut(s) 498, 1080, 1164, 1232, 1368, 2257
BlsI GCNGC 6 cut(s) 499, 1081, 1165, 1233, 1369, 2258
Bme1390I CCNGG 8 cut(s) 309, 437, 459, 633, 1240, 1777, 1929, 2016
Bme18I GGWCC 7 cut(s) 398, 569, 605, 801, 1478, 1788, 1868
BmgT120I GGNCC 8 cut(s) 398, 569, 605, 801, 1478, 1730, 1788, 1868
BmiI GGNNCC 6 cut(s) 190, 571, 628, 1026, 2117, 2260
BmrFI CCNGG 8 cut(s) 309, 437, 459, 633, 1240, 1777, 1929, 2016
BmsI GCATC 7 cut(s) 706, 982, 1354, 1426, 1607, 1653, 2007
BmtI GCTAGC 1 cut(s) 1962
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 2243, 2275
BpmI CTGGAG 2 cut(s) 705, 1740
Bpu10I CCTNAGC 1 cut(s) 1074
BpuMI CCSGG 5 cut(s) 309, 437, 459, 1777, 1929
Bsa29I ATCGAT 2 cut(s) 735, 1527
BsaBI GATNNNNATC 1 cut(s) 1597
BsaHI GRCGYC 2 cut(s) 627, 2005
BsaJI CCNNGG 5 cut(s) 308, 631, 692, 1019, 1238
BsaWI WCCGGW 1 cut(s) 607
Bsc4I CCNNNNNNNGG 9 cut(s) 308, 562, 798, 810, 1312, 1344, 1691, 1857, 1899
Bse118I RCCGGY 3 cut(s) 607, 1309, 1732
Bse1I ACTGG 6 cut(s) 688, 820, 1291, 1325, 1804, 2015
Bse3DI GCAATG 2 cut(s) 507, 1668
Bse8I GATNNNNATC 1 cut(s) 1597
BseBI CCWGG 3 cut(s) 633, 1240, 2016
BseCI ATCGAT 2 cut(s) 735, 1527
BseDI CCNNGG 5 cut(s) 308, 631, 692, 1019, 1238
BseGI GGATG 5 cut(s) 192, 268, 721, 1408, 1441
BseJI GATNNNNATC 1 cut(s) 1597
BseLI CCNNNNNNNGG 9 cut(s) 308, 562, 798, 810, 1312, 1344, 1691, 1857, 1899
BseMI GCAATG 2 cut(s) 507, 1668
BseMII CTCAG 5 cut(s) 207, 575, 599, 1065, 1648
BseNI ACTGG 6 cut(s) 688, 820, 1291, 1325, 1804, 2015
BseSI GKGCMC 1 cut(s) 1701
BseXI GCAGC 5 cut(s) 509, 1091, 1150, 1218, 1354
BsgI GTGCAG 2 cut(s) 56, 843
BshFI GGCC 6 cut(s) 226, 375, 963, 1299, 1732, 2014
BshNI GGYRCC 2 cut(s) 626, 2115
BshTI ACCGGT 1 cut(s) 607
BshVI ATCGAT 2 cut(s) 735, 1527
BsiHKAI GWGCWC 1 cut(s) 1701
BsiSI CCGG 8 cut(s) 308, 437, 458, 608, 1310, 1733, 1777, 1928
BslFI GGGAC 4 cut(s) 555, 674, 1759, 1781
BslI CCNNNNNNNGG 9 cut(s) 308, 562, 798, 810, 1312, 1344, 1691, 1857, 1899
BsmAI GTCTC 2 cut(s) 588, 1700
BsmFI GGGAC 4 cut(s) 555, 674, 1759, 1781
BsmI GAATGC 2 cut(s) 1251, 1720
BsnI GGCC 6 cut(s) 226, 375, 963, 1299, 1732, 2014
Bsp1286I GDGCHC 4 cut(s) 11, 1307, 1410, 1701
Bsp143I GATC 3 cut(s) 1096, 1505, 1983
Bsp19I CCATGG 1 cut(s) 692
BspACI CCGC 7 cut(s) 26, 391, 837, 1307, 2126, 2181, 2256
BspANI GGCC 6 cut(s) 226, 375, 963, 1299, 1732, 2014
BspCNI CTCAG 5 cut(s) 208, 574, 598, 1066, 1647
BspDI ATCGAT 2 cut(s) 735, 1527
BspLI GGNNCC 6 cut(s) 190, 571, 628, 1026, 2117, 2260
BspMAI CTGCAG 1 cut(s) 1105
BspMI ACCTGC 1 cut(s) 865
BspOI GCTAGC 1 cut(s) 1962
BspPI GGATC 3 cut(s) 1091, 1513, 1991
BspT107I GGYRCC 2 cut(s) 626, 2115
BsrDI GCAATG 2 cut(s) 507, 1668
BsrFI RCCGGY 3 cut(s) 607, 1309, 1732
BsrI ACTGG 6 cut(s) 688, 820, 1291, 1325, 1804, 2015
BssAI RCCGGY 3 cut(s) 607, 1309, 1732
BssECI CCNNGG 5 cut(s) 308, 631, 692, 1019, 1238
BssMI GATC 3 cut(s) 1096, 1505, 1983
BssNAI GTATAC 1 cut(s) 825
BssNI GRCGYC 2 cut(s) 627, 2005
BssT1I CCWWGG 2 cut(s) 692, 1019
Bst1107I GTATAC 1 cut(s) 825
Bst2UI CCWGG 3 cut(s) 633, 1240, 2016
Bst4CI ACNGT 8 cut(s) 53, 552, 749, 758, 1393, 1582, 1627, 1834
Bst6I CTCTTC 4 cut(s) 164, 1378, 1482, 1521
BstACI GRCGYC 2 cut(s) 627, 2005
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstC8I GCNNGC 7 cut(s) 7, 373, 707, 1307, 1642, 1656, 1960
BstDEI CTNAG 8 cut(s) 216, 561, 585, 1074, 1634, 1664, 2193, 2227
BstDSI CCRYGG 1 cut(s) 692
BstEII GGTNACC 1 cut(s) 2183
BstF5I GGATG 5 cut(s) 192, 268, 721, 1408, 1441
BstH2I RGCGCY 2 cut(s) 69, 630
BstHHI GCGC 2 cut(s) 68, 629
BstKTI GATC 3 cut(s) 1099, 1508, 1986
BstMAI GTCTC 2 cut(s) 588, 1700
BstMBI GATC 3 cut(s) 1096, 1505, 1983
BstNI CCWGG 3 cut(s) 633, 1240, 2016
BstNSI RCATGY 5 cut(s) 82, 928, 1544, 1553, 1774
BstPI GGTNACC 1 cut(s) 2183
BstSCI CCNGG 8 cut(s) 307, 435, 457, 631, 1238, 1775, 1927, 2014
BstSFI CTRYAG 2 cut(s) 120, 1101
BstSLI GKGCMC 1 cut(s) 1701
BstV1I GCAGC 5 cut(s) 509, 1091, 1150, 1218, 1354
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 1505
BstXI CCANNNNNNTGG 1 cut(s) 2015
BstYI RGATCY 1 cut(s) 1505
BstZ17I GTATAC 1 cut(s) 825
Bsu15I ATCGAT 2 cut(s) 735, 1527
BsuI GTATCC 1 cut(s) 2012
BsuRI GGCC 6 cut(s) 226, 375, 963, 1299, 1732, 2014
BsuTUI ATCGAT 2 cut(s) 735, 1527
BtgI CCRYGG 1 cut(s) 692
BtsCI GGATG 5 cut(s) 192, 268, 721, 1408, 1441
BtsI GCAGTG 1 cut(s) 1110
BtsIMutI CAGTG 5 cut(s) 681, 1110, 1284, 1623, 2008
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 7 cut(s) 7, 373, 707, 1307, 1642, 1656, 1960
CaiI CAGNNNCTG 2 cut(s) 128, 1580
CfoI GCGC 2 cut(s) 68, 629
Cfr10I RCCGGY 3 cut(s) 607, 1309, 1732
Cfr13I GGNCC 8 cut(s) 398, 569, 605, 801, 1478, 1730, 1788, 1868
ClaI ATCGAT 2 cut(s) 735, 1527
CseI GACGC 1 cut(s) 2013
Csp6I GTAC 5 cut(s) 362, 1354, 1456, 1903, 2116
CspAI ACCGGT 1 cut(s) 607
CviQI GTAC 5 cut(s) 362, 1354, 1456, 1903, 2116
DdeI CTNAG 8 cut(s) 216, 561, 585, 1074, 1634, 1664, 2193, 2227
DinI GGCGCC 1 cut(s) 628
DpnI GATC 3 cut(s) 1098, 1507, 1985
DpnII GATC 3 cut(s) 1096, 1505, 1983
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 1 cut(s) 1297
Eam1104I CTCTTC 4 cut(s) 164, 1378, 1482, 1521
EarI CTCTTC 4 cut(s) 164, 1378, 1482, 1521
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 692, 1019
Eco147I AGGCCT 1 cut(s) 375
Eco24I GRGCYC 3 cut(s) 11, 1307, 1410
Eco47I GGWCC 7 cut(s) 398, 569, 605, 801, 1478, 1788, 1868
Eco57I CTGAAG 1 cut(s) 1293
Eco91I GGTNACC 1 cut(s) 2183
EcoO109I RGGNCCY 2 cut(s) 569, 1478
EcoO65I GGTNACC 1 cut(s) 2183
EcoRI GAATTC 2 cut(s) 174, 1753
EcoRII CCWGG 3 cut(s) 631, 1238, 2014
EcoT14I CCWWGG 2 cut(s) 692, 1019
EcoT38I GRGCYC 3 cut(s) 11, 1307, 1410
EgeI GGCGCC 1 cut(s) 628
EheI GGCGCC 1 cut(s) 628
ErhI CCWWGG 2 cut(s) 692, 1019
FaqI GGGAC 4 cut(s) 555, 674, 1759, 1781
FauI CCCGC 2 cut(s) 830, 1314
FblI GTMKAC 1 cut(s) 824
Fnu4HI GCNGC 6 cut(s) 498, 1080, 1164, 1232, 1368, 2257
FokI GGATG 5 cut(s) 179, 275, 728, 1415, 1448
FriOI GRGCYC 3 cut(s) 11, 1307, 1410
Fsp4HI GCNGC 6 cut(s) 498, 1080, 1164, 1232, 1368, 2257
FspBI CTAG 6 cut(s) 852, 978, 1001, 1431, 1727, 1959
GlaI GCGC 2 cut(s) 67, 628
GluI GCNGC 6 cut(s) 498, 1080, 1164, 1232, 1368, 2257
GsuI CTGGAG 2 cut(s) 705, 1740
HaeII RGCGCY 2 cut(s) 69, 630
HaeIII GGCC 6 cut(s) 226, 375, 963, 1299, 1732, 2014
HapII CCGG 8 cut(s) 308, 437, 458, 608, 1310, 1733, 1777, 1928
HgaI GACGC 1 cut(s) 2013
HhaI GCGC 2 cut(s) 68, 629
Hin1I GRCGYC 2 cut(s) 627, 2005
Hin6I GCGC 2 cut(s) 66, 627
HinP1I GCGC 2 cut(s) 66, 627
HincII GTYRAC 1 cut(s) 1568
HindII GTYRAC 1 cut(s) 1568
HindIII AAGCTT 1 cut(s) 1652
HinfI GANTC 5 cut(s) 432, 687, 1036, 1123, 1529
HpaII CCGG 8 cut(s) 308, 437, 458, 608, 1310, 1733, 1777, 1928
HphI GGTGA 2 cut(s) 1823, 2195
Hpy166II GTNNAC 5 cut(s) 801, 813, 825, 1568, 1699
Hpy188I TCNGA 7 cut(s) 588, 1044, 1521, 1637, 1752, 1995, 2003
Hpy188III TCNNGA 8 cut(s) 238, 722, 1001, 1127, 1200, 1259, 1575, 1758
Hpy8I GTNNAC 5 cut(s) 801, 813, 825, 1568, 1699
HpyAV CCTTC 6 cut(s) 235, 312, 532, 1284, 1738, 1859
HpyCH4III ACNGT 8 cut(s) 53, 552, 749, 758, 1393, 1582, 1627, 1834
HpyF3I CTNAG 8 cut(s) 216, 561, 585, 1074, 1634, 1664, 2193, 2227
Hsp92I GRCGYC 2 cut(s) 627, 2005
HspAI GCGC 2 cut(s) 66, 627
KasI GGCGCC 1 cut(s) 626
KpnI GGTACC 1 cut(s) 2119
Kzo9I GATC 3 cut(s) 1096, 1505, 1983
LmnI GCTCC 3 cut(s) 724, 1942, 2264
Lsp1109I GCAGC 5 cut(s) 509, 1091, 1150, 1218, 1354
LweI GCATC 7 cut(s) 706, 982, 1354, 1426, 1607, 1653, 2007
MaeI CTAG 6 cut(s) 852, 978, 1001, 1431, 1727, 1959
MaeIII GTNAC 6 cut(s) 53, 1105, 1393, 1735, 2103, 2183
MalI GATC 3 cut(s) 1098, 1507, 1985
MboI GATC 3 cut(s) 1096, 1505, 1983
MboII GAAGA 9 cut(s) 105, 181, 1247, 1365, 1373, 1499, 1508, 1839, 2183
MfeI CAATTG 3 cut(s) 649, 2044, 2279
MflI RGATCY 1 cut(s) 1505
MhlI GDGCHC 4 cut(s) 11, 1307, 1410, 1701
MlsI TGGCCA 1 cut(s) 1299
MluNI TGGCCA 1 cut(s) 1299
Mly113I GGCGCC 1 cut(s) 627
MlyI GAGTC 3 cut(s) 696, 1045, 1132
MmeI TCCRAC 1 cut(s) 2286
Mox20I TGGCCA 1 cut(s) 1299
MroXI GAANNNNTTC 2 cut(s) 1491, 1846
MscI TGGCCA 1 cut(s) 1299
MseI TTAA 6 cut(s) 233, 345, 1610, 1617, 1820, 1836
MslI CAYNNNNRTG 1 cut(s) 2110
Msp20I TGGCCA 1 cut(s) 1299
MspA1I CMGCKG 1 cut(s) 1535
MspI CCGG 8 cut(s) 308, 437, 458, 608, 1310, 1733, 1777, 1928
MspR9I CCNGG 8 cut(s) 309, 437, 459, 633, 1240, 1777, 1929, 2016
MunI CAATTG 3 cut(s) 649, 2044, 2279
Mva1269I GAATGC 2 cut(s) 1251, 1720
MvaI CCWGG 3 cut(s) 633, 1240, 2016
NarI GGCGCC 1 cut(s) 627
NciI CCSGG 5 cut(s) 309, 437, 459, 1777, 1929
NcoI CCATGG 1 cut(s) 692
NdeII GATC 3 cut(s) 1096, 1505, 1983
NheI GCTAGC 1 cut(s) 1958
NlaIV GGNNCC 6 cut(s) 190, 571, 628, 1026, 2117, 2260
NmuCI GTSAC 4 cut(s) 1105, 1735, 2103, 2183
NspI RCATGY 5 cut(s) 82, 928, 1544, 1553, 1774
PceI AGGCCT 1 cut(s) 375
PciI ACATGT 3 cut(s) 78, 924, 1770
PctI GAATGC 2 cut(s) 1251, 1720
PdmI GAANNNNTTC 2 cut(s) 1491, 1846
PfeI GAWTC 2 cut(s) 432, 1529
PflMI CCANNNNNTGG 1 cut(s) 798
PfoI TCCNGGA 1 cut(s) 457
PinAI ACCGGT 1 cut(s) 607
PkrI GCNGC 6 cut(s) 499, 1081, 1165, 1233, 1369, 2258
PleI GAGTC 3 cut(s) 695, 1044, 1131
PluTI GGCGCC 1 cut(s) 630
PpsI GAGTC 3 cut(s) 695, 1044, 1131
PpuMI RGGWCCY 2 cut(s) 569, 1478
PscI ACATGT 3 cut(s) 78, 924, 1770
Psp5II RGGWCCY 2 cut(s) 569, 1478
Psp6I CCWGG 3 cut(s) 631, 1238, 2014
PspEI GGTNACC 1 cut(s) 2183
PspGI CCWGG 3 cut(s) 631, 1238, 2014
PspN4I GGNNCC 6 cut(s) 190, 571, 628, 1026, 2117, 2260
PspPI GGNCC 8 cut(s) 398, 569, 605, 801, 1478, 1730, 1788, 1868
PspPPI RGGWCCY 2 cut(s) 569, 1478
PstI CTGCAG 1 cut(s) 1105
PstNI CAGNNNCTG 2 cut(s) 128, 1580
PsuI RGATCY 1 cut(s) 1505
PvuII CAGCTG 1 cut(s) 1535
RsaI GTAC 5 cut(s) 363, 1355, 1457, 1904, 2117
RsaNI GTAC 5 cut(s) 362, 1354, 1456, 1903, 2116
RseI CAYNNNNRTG 1 cut(s) 2110
SaqAI TTAA 6 cut(s) 233, 345, 1610, 1617, 1820, 1836
SatI GCNGC 6 cut(s) 498, 1080, 1164, 1232, 1368, 2257
Sau3AI GATC 3 cut(s) 1096, 1505, 1983
Sau96I GGNCC 8 cut(s) 398, 569, 605, 801, 1478, 1730, 1788, 1868
SchI GAGTC 3 cut(s) 696, 1045, 1132
ScrFI CCNGG 8 cut(s) 309, 437, 459, 633, 1240, 1777, 1929, 2016
SduI GDGCHC 4 cut(s) 11, 1307, 1410, 1701
SfaNI GCATC 7 cut(s) 706, 982, 1354, 1426, 1607, 1653, 2007
SfcI CTRYAG 2 cut(s) 120, 1101
SfoI GGCGCC 1 cut(s) 628
SgrAI CRCCGGYG 1 cut(s) 1309
SinI GGWCC 7 cut(s) 398, 569, 605, 801, 1478, 1788, 1868
SmiMI CAYNNNNRTG 1 cut(s) 2110
SseBI AGGCCT 1 cut(s) 375
SsiI CCGC 7 cut(s) 26, 391, 837, 1307, 2126, 2181, 2256
SspDI GGCGCC 1 cut(s) 626
SspI AATATT 1 cut(s) 1049
SspMI CTAG 6 cut(s) 852, 978, 1001, 1431, 1727, 1959
StuI AGGCCT 1 cut(s) 375
StyD4I CCNGG 8 cut(s) 307, 435, 457, 631, 1238, 1775, 1927, 2014
StyI CCWWGG 2 cut(s) 692, 1019
TaaI ACNGT 8 cut(s) 53, 552, 749, 758, 1393, 1582, 1627, 1834
TaqI TCGA 3 cut(s) 735, 1359, 1527
TaqII GACCGA 2 cut(s) 818, 966
TauI GCSGC 1 cut(s) 2259
TfiI GAWTC 2 cut(s) 432, 1529
Tru1I TTAA 6 cut(s) 233, 345, 1610, 1617, 1820, 1836
Tru9I TTAA 6 cut(s) 233, 345, 1610, 1617, 1820, 1836
TscAI CASTG 5 cut(s) 688, 1110, 1291, 1630, 2015
TseFI GTSAC 4 cut(s) 1105, 1735, 2103, 2183
TseI GCWGC 5 cut(s) 497, 1079, 1163, 1231, 1367
Tsp45I GTSAC 4 cut(s) 1105, 1735, 2103, 2183
TspGWI ACGGA 1 cut(s) 1840
TspRI CASTG 5 cut(s) 688, 1110, 1291, 1630, 2015
Van91I CCANNNNNTGG 1 cut(s) 798
VneI GTGCAC 1 cut(s) 1697
VpaK11BI GGWCC 7 cut(s) 398, 569, 605, 801, 1478, 1788, 1868
XapI RAATTY 4 cut(s) 174, 830, 1753, 2152
XbaI TCTAGA 1 cut(s) 1000
XceI RCATGY 5 cut(s) 82, 928, 1544, 1553, 1774
XmiI GTMKAC 1 cut(s) 824
XmnI GAANNNNTTC 2 cut(s) 1491, 1846
XspI CTAG 6 cut(s) 852, 978, 1001, 1431, 1727, 1959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.