Rh2AG080800

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002889
Physical Location & Seq
Forward (+)
6767753 .. 6774303
6551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG080800.1

Sequence Viewer

Length: 2262 bp
ATGGATTTAGTGCAGTCAATGTGGAACGGTAACAAGTTGAGCGCCAATGCTAACATGTTCCTGCTATTGTTTCTGTTTTCGTGGCTGTCTTCTGCTACAGCCTCTGTGAGTTATGACCACAGAGCTATAATAATTGATGGACAGAAGAGAATTCTCATTTCTGGTTCCATCCACTATCCCAGAAGCACACCTGAGATGTGGCCTGATTTAATCCAGAAGGCTAAAGATGGAGGCTTGGATGTTATACAAACCTATGTGTTTTGGAACGGGCATGAGCCTTCTCCGGGGAAGTATTATTTTGAGGATAGATATGATTTGGTTAAGTTCATCAAGCTGGTACAAAAAGCAGGCCTGTATGTTCATCTCCGCATTGGTCCTTATGTTTGTGCTGAATGGAACTTTGGGGGATTCCCTGCTTGGCTCAAATATGTTCCGGGAATTGAGTTTAGAACAGACAATGAGCCTTTCAAAGCAGCAATGAAAACATTTACAGAGAAAATTGTCGGTATGATGAAGGCAGAACGACTGTTCCAAACTCAGGGAGGTCCCATTATTCTGTCTCAGATAGAAAATGAGTATGGACCGGTGGAATGGACAATCGGCGCCCCTGGTAAAGCATATACCAATTGGACAGCACAAATGGCTCTTGGGCTTGACACTGGAGTCCCATGGGTAATGTGCAAGCAAGAGGATGCTCCTGACCCAATTATCGATACTTGCAACGGTTTCTACTGTGAAAACTTCACTCCAAACAAGAAGTATAAACCCAAAATGTGGACCGAAGTGTGGACTGGCTGGTATACAGAATTTGGCGGTGCAGTTCCTACTAGACCTGCACAAGATTTGGCATTTTCAGTTGCAAGGTTTATACAAAATGGTGGCTCTTTTGCTAACTATTACATGTATCATGGAGGAACTAATTTTGACCGAACTTCTGGCCTTTTCGTTGCAACTAGCTATGACTATGATGCACCTCTAGATGAATATGGACTACCAAGGGAACCAAAGTGGAGTCATCTGAAATATTTGCACAAAGCCATCAAATTGGCTGAGGCAGCTTTAGTGGCTACTGATCCTGCAGTGACATCACTTGGAAAGAGTCAAGAGGCTCATGTATTCAATTCAAGTTCTGGGTGTGCTGCATTTCTTGCAAATTACGACACAAAATACTCGTCAAGAGTGAGCTTTGGGAATAGGCAATATAAGCTGCCAGCCTGGTCTATAAGCATTCTTCCTGACTGCAAAACTGAAGTTCTCAACACTGGAAGGGTTGGCCAGACCCCGCCGGTGGATATGACACCAGTAAATACCACCCTTTCTTGGCAGTCGTACATCGAAGATGCTGCCTCTTCTACTGATGACAATACATTTACATTGGATGGGCTCAATGAGCAAATAAATATCACTAGGGATGCAACAGACTACTTATGGTACATGACAGACATCACAATAGGTCCTAATGAAGAGTTTCTAAAGAATGGATCTTACCCTCTTCTGACTATCGATTCAGCTGGACATGCCTTGCATGTTTTCATCAATGGTCAACTATCAGGAACTGTTTATGCAGGATTGGCATCTCCTAAATTAAGATTTAATGACACTGTGAAGCTCAGAGCTGGCATCAACAAGCTTGCTTTACTAAGCATTGCTGTTGGTCTGCCGAATGTTGGTGTGCACTTTGAGACTTGGAATGCTGGAGTTCTAGGGCCGGTCACATTAAAGGGTCTGAATTCGGGAACATGGGACATGTCCCAGTGGAAATGGACCTACAAGACTGGTATGAAAGGTGAAGCATTAAGCCTCCATACTGTTAAGGGAAGTTCTTCCGTTGAATGGGTAGAAGGACCACTATTGGCTCAGAAACAACCCCTTACATGGTACAAGTCTATATTTCATGCACCACCGGGAAATGCTCCATTAGCTTTAGATATGGTTAGCATGGGCAAAGGTCAAGTATGGATCAATGGTCAGAGCATCGGACGCCACTGGCCTGGATACATAGCACATGGTAGCTGTGGCAATTGTTCTTATGCTGGAACTTACACCGAAAACAAATGCCGAACAAATTGTGGTCAGCCGTCACAGAGATGGTACCATGTTCCACAATCGTGGTTGAACCAGAGTGAAAATTTGTTGGTGGTGTTTGAAGAATGGGGTGGTGACCCGACTACGATTTCTCTGGTGGCACGAAGCTATGAAAACTTAGGGCAGACCCTCTATAACATAGAGGGCGGCTCCAACCATTTGAGATTGCAATTGTAG

Protein Analysis

753

Amino Acids

83.95

Weight (kDa)

6.3

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 43 - 346 2.3e-117 Glycosyl hydrolases family 35
GHD PF17834 369 - 424 1.3e-19 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 475 - 523 5.6e-06 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 624 - 710 2.6e-24 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
Acc36I ACCTGC 1 cut(s) 841
Acc65I GGTACC 1 cut(s) 2091
AccB1I GGYRCC 2 cut(s) 602, 2091
AccB7I CCANNNNNTGG 1 cut(s) 774
AccI GTMKAC 1 cut(s) 800
AciI CCGC 4 cut(s) 367, 813, 1283, 2232
AclWI GGATC 3 cut(s) 1067, 1489, 1967
AcoI YGGCCR 1 cut(s) 1273
AcsI RAATTY 4 cut(s) 150, 806, 1729, 2128
AcuI CTGAAG 1 cut(s) 1269
AcyI GRCGYC 2 cut(s) 603, 1981
AfaI GTAC 5 cut(s) 339, 1331, 1433, 1880, 2093
AfiI CCNNNNNNNGG 9 cut(s) 284, 538, 774, 786, 1288, 1320, 1667, 1833, 1875
AflIII ACRYGT 3 cut(s) 54, 900, 1746
AgeI ACCGGT 1 cut(s) 583
AgsI TTSAA 6 cut(s) 469, 1120, 1125, 1832, 2116, 2147
AjnI CCWGG 3 cut(s) 607, 1214, 1990
AjuI GAANNNNNNNTTGG 2 cut(s) 384, 416
AleI CACNNNNGTG 1 cut(s) 2107
AloI GAACNNNNNNTCC 2 cut(s) 513, 545
Alw21I GWGCWC 1 cut(s) 1677
Alw26I GTCTC 2 cut(s) 564, 1676
Alw44I GTGCAC 1 cut(s) 1673
AlwI GGATC 3 cut(s) 1067, 1489, 1967
AlwNI CAGNNNCTG 2 cut(s) 104, 1556
AoxI GGCC 6 cut(s) 200, 349, 937, 1273, 1706, 1988
ApaLI GTGCAC 1 cut(s) 1673
ApeKI GCWGC 5 cut(s) 473, 1055, 1139, 1207, 1343
ApoI RAATTY 4 cut(s) 150, 806, 1729, 2128
ArsI GACNNNNNNTTYG 2 cut(s) 1157, 1189
AsiGI ACCGGT 1 cut(s) 583
Asp700I GAANNNNTTC 2 cut(s) 1467, 1822
Asp718I GGTACC 1 cut(s) 2091
AspLEI GCGC 2 cut(s) 44, 605
AspS9I GGNCC 8 cut(s) 374, 545, 581, 777, 1454, 1706, 1764, 1844
AsuC2I CCSGG 3 cut(s) 285, 435, 1905
AsuHPI GGTGA 2 cut(s) 1799, 2171
AvaII GGWCC 7 cut(s) 374, 545, 581, 777, 1454, 1764, 1844
BaeGI GKGCMC 1 cut(s) 1677
BalI TGGCCA 1 cut(s) 1275
BanI GGYRCC 2 cut(s) 602, 2091
BanII GRGCYC 1 cut(s) 1386
BbsI GAAGAC 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 1677
BbvCI CCTCAGC 1 cut(s) 1050
BbvI GCAGC 5 cut(s) 485, 1067, 1126, 1194, 1330
BccI CCATC 6 cut(s) 131, 176, 221, 1046, 1373, 2082
BceAI ACGGC 1 cut(s) 2062
BcgI CGANNNNNNTGC 2 cut(s) 1325, 1359
BciT130I CCWGG 3 cut(s) 609, 1216, 1992
BciVI GTATCC 1 cut(s) 1988
BcnI CCSGG 3 cut(s) 285, 435, 1905
BcoDI GTCTC 2 cut(s) 564, 1676
BfaI CTAG 5 cut(s) 828, 954, 977, 1407, 1703
BfmI CTRYAG 2 cut(s) 96, 1077
BfoI RGCGCY 2 cut(s) 45, 606
BfuAI ACCTGC 1 cut(s) 841
BfuI GTATCC 1 cut(s) 1988
BisI GCNGC 6 cut(s) 474, 1056, 1140, 1208, 1344, 2233
BlsI GCNGC 6 cut(s) 475, 1057, 1141, 1209, 1345, 2234
Bme1390I CCNGG 6 cut(s) 285, 435, 609, 1216, 1905, 1992
Bme18I GGWCC 7 cut(s) 374, 545, 581, 777, 1454, 1764, 1844
BmgT120I GGNCC 8 cut(s) 374, 545, 581, 777, 1454, 1706, 1764, 1844
BmiI GGNNCC 6 cut(s) 166, 547, 604, 1002, 2093, 2236
BmrFI CCNGG 6 cut(s) 285, 435, 609, 1216, 1905, 1992
BmrI ACTGGG 1 cut(s) 1747
BmsI GCATC 7 cut(s) 682, 958, 1330, 1402, 1583, 1629, 1983
BmuI ACTGGG 1 cut(s) 1747
BpiI GAAGAC 1 cut(s) 81
BplI GAGNNNNNCTC 2 cut(s) 2219, 2251
BpmI CTGGAG 2 cut(s) 681, 1716
Bpu10I CCTNAGC 1 cut(s) 1050
BpuMI CCSGG 3 cut(s) 285, 435, 1905
Bsa29I ATCGAT 2 cut(s) 711, 1503
BsaBI GATNNNNATC 1 cut(s) 1573
BsaHI GRCGYC 2 cut(s) 603, 1981
BsaJI CCNNGG 4 cut(s) 284, 607, 668, 995
BsaWI WCCGGW 1 cut(s) 583
Bsc4I CCNNNNNNNGG 9 cut(s) 284, 538, 774, 786, 1288, 1320, 1667, 1833, 1875
Bse118I RCCGGY 3 cut(s) 583, 1285, 1708
Bse1I ACTGG 7 cut(s) 664, 796, 1267, 1301, 1753, 1780, 1991
Bse3DI GCAATG 2 cut(s) 483, 1644
Bse8I GATNNNNATC 1 cut(s) 1573
BseBI CCWGG 3 cut(s) 609, 1216, 1992
BseCI ATCGAT 2 cut(s) 711, 1503
BseDI CCNNGG 4 cut(s) 284, 607, 668, 995
BseGI GGATG 5 cut(s) 168, 244, 697, 1384, 1417
BseJI GATNNNNATC 1 cut(s) 1573
BseLI CCNNNNNNNGG 9 cut(s) 284, 538, 774, 786, 1288, 1320, 1667, 1833, 1875
BseMI GCAATG 2 cut(s) 483, 1644
BseMII CTCAG 6 cut(s) 183, 551, 575, 1041, 1624, 1871
BseNI ACTGG 7 cut(s) 664, 796, 1267, 1301, 1753, 1780, 1991
BseSI GKGCMC 1 cut(s) 1677
BseXI GCAGC 5 cut(s) 485, 1067, 1126, 1194, 1330
BsgI GTGCAG 3 cut(s) 32, 819, 837
BshFI GGCC 6 cut(s) 202, 351, 939, 1275, 1708, 1990
BshNI GGYRCC 2 cut(s) 602, 2091
BshTI ACCGGT 1 cut(s) 583
BshVI ATCGAT 2 cut(s) 711, 1503
BsiHKAI GWGCWC 1 cut(s) 1677
BsiSI CCGG 6 cut(s) 284, 434, 584, 1286, 1709, 1904
BslFI GGGAC 4 cut(s) 531, 650, 1735, 1757
BslI CCNNNNNNNGG 9 cut(s) 284, 538, 774, 786, 1288, 1320, 1667, 1833, 1875
BsmAI GTCTC 2 cut(s) 564, 1676
BsmFI GGGAC 4 cut(s) 531, 650, 1735, 1757
BsmI GAATGC 2 cut(s) 1227, 1696
BsnI GGCC 6 cut(s) 202, 351, 939, 1275, 1708, 1990
Bsp1286I GDGCHC 2 cut(s) 1386, 1677
Bsp143I GATC 3 cut(s) 1072, 1481, 1959
Bsp19I CCATGG 1 cut(s) 668
BspACI CCGC 4 cut(s) 367, 813, 1283, 2232
BspANI GGCC 6 cut(s) 202, 351, 939, 1275, 1708, 1990
BspCNI CTCAG 6 cut(s) 184, 550, 574, 1042, 1623, 1870
BspDI ATCGAT 2 cut(s) 711, 1503
BspLI GGNNCC 6 cut(s) 166, 547, 604, 1002, 2093, 2236
BspMAI CTGCAG 1 cut(s) 1081
BspMI ACCTGC 1 cut(s) 841
BspPI GGATC 3 cut(s) 1067, 1489, 1967
BspT107I GGYRCC 2 cut(s) 602, 2091
BsrDI GCAATG 2 cut(s) 483, 1644
BsrFI RCCGGY 3 cut(s) 583, 1285, 1708
BsrI ACTGG 7 cut(s) 664, 796, 1267, 1301, 1753, 1780, 1991
BssAI RCCGGY 3 cut(s) 583, 1285, 1708
BssECI CCNNGG 4 cut(s) 284, 607, 668, 995
BssMI GATC 3 cut(s) 1072, 1481, 1959
BssNAI GTATAC 1 cut(s) 801
BssNI GRCGYC 2 cut(s) 603, 1981
BssT1I CCWWGG 2 cut(s) 668, 995
Bst1107I GTATAC 1 cut(s) 801
Bst2UI CCWGG 3 cut(s) 609, 1216, 1992
Bst4CI ACNGT 7 cut(s) 29, 528, 725, 734, 1558, 1603, 1810
Bst6I CTCTTC 4 cut(s) 140, 1354, 1458, 1497
BstACI GRCGYC 2 cut(s) 603, 1981
BstAPI GCANNNNNTGC 1 cut(s) 1148
BstC8I GCNNGC 5 cut(s) 349, 683, 1212, 1618, 1632
BstDEI CTNAG 8 cut(s) 192, 537, 561, 1050, 1610, 1640, 1857, 2203
BstDSI CCRYGG 1 cut(s) 668
BstEII GGTNACC 1 cut(s) 2159
BstF5I GGATG 5 cut(s) 168, 244, 697, 1384, 1417
BstH2I RGCGCY 2 cut(s) 45, 606
BstHHI GCGC 2 cut(s) 44, 605
BstKTI GATC 3 cut(s) 1075, 1484, 1962
BstMAI GTCTC 2 cut(s) 564, 1676
BstMBI GATC 3 cut(s) 1072, 1481, 1959
BstNI CCWGG 3 cut(s) 609, 1216, 1992
BstNSI RCATGY 5 cut(s) 58, 904, 1520, 1529, 1750
BstPI GGTNACC 1 cut(s) 2159
BstSCI CCNGG 6 cut(s) 283, 433, 607, 1214, 1903, 1990
BstSFI CTRYAG 2 cut(s) 96, 1077
BstSLI GKGCMC 1 cut(s) 1677
BstV1I GCAGC 5 cut(s) 485, 1067, 1126, 1194, 1330
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 1481
BstXI CCANNNNNNTGG 3 cut(s) 1045, 1991, 2109
BstYI RGATCY 1 cut(s) 1481
BstZ17I GTATAC 1 cut(s) 801
Bsu15I ATCGAT 2 cut(s) 711, 1503
BsuI GTATCC 1 cut(s) 1988
BsuRI GGCC 6 cut(s) 202, 351, 939, 1275, 1708, 1990
BsuTUI ATCGAT 2 cut(s) 711, 1503
BtgI CCRYGG 1 cut(s) 668
BtsCI GGATG 5 cut(s) 168, 244, 697, 1384, 1417
BtsI GCAGTG 1 cut(s) 1086
BtsIMutI CAGTG 6 cut(s) 657, 1086, 1260, 1599, 1760, 1984
BveI ACCTGC 1 cut(s) 841
Cac8I GCNNGC 5 cut(s) 349, 683, 1212, 1618, 1632
CaiI CAGNNNCTG 2 cut(s) 104, 1556
CfoI GCGC 2 cut(s) 44, 605
Cfr10I RCCGGY 3 cut(s) 583, 1285, 1708
Cfr13I GGNCC 8 cut(s) 374, 545, 581, 777, 1454, 1706, 1764, 1844
ClaI ATCGAT 2 cut(s) 711, 1503
CseI GACGC 1 cut(s) 1989
Csp6I GTAC 5 cut(s) 338, 1330, 1432, 1879, 2092
CspAI ACCGGT 1 cut(s) 583
CviQI GTAC 5 cut(s) 338, 1330, 1432, 1879, 2092
DdeI CTNAG 8 cut(s) 192, 537, 561, 1050, 1610, 1640, 1857, 2203
DinI GGCGCC 1 cut(s) 604
DpnI GATC 3 cut(s) 1074, 1483, 1961
DpnII GATC 3 cut(s) 1072, 1481, 1959
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
EaeI YGGCCR 1 cut(s) 1273
Eam1104I CTCTTC 4 cut(s) 140, 1354, 1458, 1497
EarI CTCTTC 4 cut(s) 140, 1354, 1458, 1497
Eco130I CCWWGG 2 cut(s) 668, 995
Eco147I AGGCCT 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 1386
Eco47I GGWCC 7 cut(s) 374, 545, 581, 777, 1454, 1764, 1844
Eco57I CTGAAG 1 cut(s) 1269
Eco91I GGTNACC 1 cut(s) 2159
EcoO109I RGGNCCY 2 cut(s) 545, 1454
EcoO65I GGTNACC 1 cut(s) 2159
EcoRI GAATTC 2 cut(s) 150, 1729
EcoRII CCWGG 3 cut(s) 607, 1214, 1990
EcoT14I CCWWGG 2 cut(s) 668, 995
EcoT38I GRGCYC 1 cut(s) 1386
EgeI GGCGCC 1 cut(s) 604
EheI GGCGCC 1 cut(s) 604
ErhI CCWWGG 2 cut(s) 668, 995
FaqI GGGAC 4 cut(s) 531, 650, 1735, 1757
FauI CCCGC 1 cut(s) 1290
FblI GTMKAC 1 cut(s) 800
Fnu4HI GCNGC 6 cut(s) 474, 1056, 1140, 1208, 1344, 2233
FokI GGATG 5 cut(s) 155, 251, 704, 1391, 1424
FriOI GRGCYC 1 cut(s) 1386
Fsp4HI GCNGC 6 cut(s) 474, 1056, 1140, 1208, 1344, 2233
FspBI CTAG 5 cut(s) 828, 954, 977, 1407, 1703
GlaI GCGC 2 cut(s) 43, 604
GluI GCNGC 6 cut(s) 474, 1056, 1140, 1208, 1344, 2233
GsuI CTGGAG 2 cut(s) 681, 1716
HaeII RGCGCY 2 cut(s) 45, 606
HaeIII GGCC 6 cut(s) 202, 351, 939, 1275, 1708, 1990
HapII CCGG 6 cut(s) 284, 434, 584, 1286, 1709, 1904
HgaI GACGC 1 cut(s) 1989
HhaI GCGC 2 cut(s) 44, 605
Hin1I GRCGYC 2 cut(s) 603, 1981
Hin6I GCGC 2 cut(s) 42, 603
HinP1I GCGC 2 cut(s) 42, 603
HincII GTYRAC 1 cut(s) 1544
HindII GTYRAC 1 cut(s) 1544
HindIII AAGCTT 1 cut(s) 1628
HinfI GANTC 5 cut(s) 408, 663, 1012, 1099, 1505
HpaII CCGG 6 cut(s) 284, 434, 584, 1286, 1709, 1904
HphI GGTGA 2 cut(s) 1799, 2171
Hpy166II GTNNAC 5 cut(s) 777, 789, 801, 1544, 1675
Hpy188I TCNGA 8 cut(s) 564, 1020, 1497, 1613, 1728, 1860, 1971, 1979
Hpy188III TCNNGA 8 cut(s) 214, 698, 977, 1103, 1176, 1235, 1551, 1734
Hpy8I GTNNAC 5 cut(s) 777, 789, 801, 1544, 1675
HpyAV CCTTC 5 cut(s) 211, 288, 508, 1260, 1835
HpyCH4III ACNGT 7 cut(s) 29, 528, 725, 734, 1558, 1603, 1810
HpyF3I CTNAG 8 cut(s) 192, 537, 561, 1050, 1610, 1640, 1857, 2203
Hsp92I GRCGYC 2 cut(s) 603, 1981
HspAI GCGC 2 cut(s) 42, 603
KasI GGCGCC 1 cut(s) 602
KpnI GGTACC 1 cut(s) 2095
Kzo9I GATC 3 cut(s) 1072, 1481, 1959
LmnI GCTCC 3 cut(s) 700, 1918, 2240
Lsp1109I GCAGC 5 cut(s) 485, 1067, 1126, 1194, 1330
LweI GCATC 7 cut(s) 682, 958, 1330, 1402, 1583, 1629, 1983
MaeI CTAG 5 cut(s) 828, 954, 977, 1407, 1703
MaeIII GTNAC 5 cut(s) 29, 1081, 1711, 2079, 2159
MalI GATC 3 cut(s) 1074, 1483, 1961
MboI GATC 3 cut(s) 1072, 1481, 1959
MboII GAAGA 9 cut(s) 81, 157, 1223, 1341, 1349, 1475, 1484, 1815, 2159
MfeI CAATTG 3 cut(s) 625, 2020, 2255
MflI RGATCY 1 cut(s) 1481
MhlI GDGCHC 2 cut(s) 1386, 1677
MlsI TGGCCA 1 cut(s) 1275
MluNI TGGCCA 1 cut(s) 1275
Mly113I GGCGCC 1 cut(s) 603
MlyI GAGTC 3 cut(s) 672, 1021, 1108
MmeI TCCRAC 1 cut(s) 2262
Mox20I TGGCCA 1 cut(s) 1275
MroXI GAANNNNTTC 2 cut(s) 1467, 1822
MscI TGGCCA 1 cut(s) 1275
MseI TTAA 7 cut(s) 209, 321, 1586, 1593, 1718, 1796, 1812
MslI CAYNNNNRTG 2 cut(s) 2086, 2107
Msp20I TGGCCA 1 cut(s) 1275
MspA1I CMGCKG 1 cut(s) 1511
MspI CCGG 6 cut(s) 284, 434, 584, 1286, 1709, 1904
MspR9I CCNGG 6 cut(s) 285, 435, 609, 1216, 1905, 1992
MunI CAATTG 3 cut(s) 625, 2020, 2255
Mva1269I GAATGC 2 cut(s) 1227, 1696
MvaI CCWGG 3 cut(s) 609, 1216, 1992
NarI GGCGCC 1 cut(s) 603
NciI CCSGG 3 cut(s) 285, 435, 1905
NcoI CCATGG 1 cut(s) 668
NdeII GATC 3 cut(s) 1072, 1481, 1959
NlaIV GGNNCC 6 cut(s) 166, 547, 604, 1002, 2093, 2236
NmuCI GTSAC 4 cut(s) 1081, 1711, 2079, 2159
NspI RCATGY 5 cut(s) 58, 904, 1520, 1529, 1750
OliI CACNNNNGTG 1 cut(s) 2107
PceI AGGCCT 1 cut(s) 351
PciI ACATGT 3 cut(s) 54, 900, 1746
PctI GAATGC 2 cut(s) 1227, 1696
PdmI GAANNNNTTC 2 cut(s) 1467, 1822
PfeI GAWTC 2 cut(s) 408, 1505
PflMI CCANNNNNTGG 1 cut(s) 774
PfoI TCCNGGA 1 cut(s) 433
PinAI ACCGGT 1 cut(s) 583
PkrI GCNGC 6 cut(s) 475, 1057, 1141, 1209, 1345, 2234
PleI GAGTC 3 cut(s) 671, 1020, 1107
PluTI GGCGCC 1 cut(s) 606
PpsI GAGTC 3 cut(s) 671, 1020, 1107
PpuMI RGGWCCY 2 cut(s) 545, 1454
PscI ACATGT 3 cut(s) 54, 900, 1746
Psp5II RGGWCCY 2 cut(s) 545, 1454
Psp6I CCWGG 3 cut(s) 607, 1214, 1990
PspEI GGTNACC 1 cut(s) 2159
PspGI CCWGG 3 cut(s) 607, 1214, 1990
PspN4I GGNNCC 6 cut(s) 166, 547, 604, 1002, 2093, 2236
PspPI GGNCC 8 cut(s) 374, 545, 581, 777, 1454, 1706, 1764, 1844
PspPPI RGGWCCY 2 cut(s) 545, 1454
PstI CTGCAG 1 cut(s) 1081
PstNI CAGNNNCTG 2 cut(s) 104, 1556
PsuI RGATCY 1 cut(s) 1481
PvuII CAGCTG 1 cut(s) 1511
RsaI GTAC 5 cut(s) 339, 1331, 1433, 1880, 2093
RsaNI GTAC 5 cut(s) 338, 1330, 1432, 1879, 2092
RseI CAYNNNNRTG 2 cut(s) 2086, 2107
SaqAI TTAA 7 cut(s) 209, 321, 1586, 1593, 1718, 1796, 1812
SatI GCNGC 6 cut(s) 474, 1056, 1140, 1208, 1344, 2233
Sau3AI GATC 3 cut(s) 1072, 1481, 1959
Sau96I GGNCC 8 cut(s) 374, 545, 581, 777, 1454, 1706, 1764, 1844
SchI GAGTC 3 cut(s) 672, 1021, 1108
ScrFI CCNGG 6 cut(s) 285, 435, 609, 1216, 1905, 1992
SduI GDGCHC 2 cut(s) 1386, 1677
SfaNI GCATC 7 cut(s) 682, 958, 1330, 1402, 1583, 1629, 1983
SfcI CTRYAG 2 cut(s) 96, 1077
SfoI GGCGCC 1 cut(s) 604
SgrAI CRCCGGYG 1 cut(s) 1285
SinI GGWCC 7 cut(s) 374, 545, 581, 777, 1454, 1764, 1844
SmiMI CAYNNNNRTG 2 cut(s) 2086, 2107
SseBI AGGCCT 1 cut(s) 351
SsiI CCGC 4 cut(s) 367, 813, 1283, 2232
SspDI GGCGCC 1 cut(s) 602
SspI AATATT 1 cut(s) 1025
SspMI CTAG 5 cut(s) 828, 954, 977, 1407, 1703
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 6 cut(s) 283, 433, 607, 1214, 1903, 1990
StyI CCWWGG 2 cut(s) 668, 995
TaaI ACNGT 7 cut(s) 29, 528, 725, 734, 1558, 1603, 1810
TaqI TCGA 3 cut(s) 711, 1335, 1503
TaqII GACCGA 2 cut(s) 794, 942
TauI GCSGC 1 cut(s) 2235
TfiI GAWTC 2 cut(s) 408, 1505
Tru1I TTAA 7 cut(s) 209, 321, 1586, 1593, 1718, 1796, 1812
Tru9I TTAA 7 cut(s) 209, 321, 1586, 1593, 1718, 1796, 1812
TscAI CASTG 6 cut(s) 664, 1086, 1267, 1606, 1760, 1991
TseFI GTSAC 4 cut(s) 1081, 1711, 2079, 2159
TseI GCWGC 5 cut(s) 473, 1055, 1139, 1207, 1343
Tsp45I GTSAC 4 cut(s) 1081, 1711, 2079, 2159
TspGWI ACGGA 1 cut(s) 1816
TspRI CASTG 6 cut(s) 664, 1086, 1267, 1606, 1760, 1991
Van91I CCANNNNNTGG 1 cut(s) 774
VneI GTGCAC 1 cut(s) 1673
VpaK11BI GGWCC 7 cut(s) 374, 545, 581, 777, 1454, 1764, 1844
XapI RAATTY 4 cut(s) 150, 806, 1729, 2128
XbaI TCTAGA 1 cut(s) 976
XceI RCATGY 5 cut(s) 58, 904, 1520, 1529, 1750
XmiI GTMKAC 1 cut(s) 800
XmnI GAANNNNTTC 2 cut(s) 1467, 1822
XspI CTAG 5 cut(s) 828, 954, 977, 1407, 1703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.