Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002889

Physical Location & Seq

Genomic Coordinates Forward (+)

16439434 .. 16444840

Gene Structure 5407 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1206800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2196 bp

ATGGGTGTTGGAATTCAAACAATGTGGAGCATTCTGCTATTGTTTTCCTGCATTTTTTCTGCAGCTTCAGCTTCTGTGAGTTATGACCACAAGGCTATAATAATTAATGGGCAGAAAAGGATTTTAATTTCTGGCTCCATTCACTATCCCAGAAGCACTCCCGAGATGTGGCCGGATTTAATTCAGAAGGCCAAAGATGGAGGCTTGGATGTTATACAGACCTATGTGTTTTGGAATGGCCATGAACCTTCTCCGGGAAATTATTATTTCGAGGACAGATATGATTTGGTCAAGTTTATCAAGCTGGTGCAACAAGCAGGCCTATTTGTTAGTCTCCGGATTGGCCCTTATGTTTGCGCTGAATGGAACTTCGGGGGATTCCCAGTTTGGCTGAAATATGTCCCTGGAATCGCTTTTCGAACGGACAATGAGCCTTTCAAGGCGGCAATGCAAAAATTTACAGAGAAGATTGTCAGCATGATGAAGGCAGAGAAGCTGTTTCAAACTCAAGGAGGTCCTATAATTCTCTCTCAGATAGAAAATGAATTTGGACCTGTGGAATGGGAAATTGGTGCACCTGGAAAAGCTTACACCAAATGGGCAGCTCAGATGGCTGTAGGCCTAGACACTGGAGTTCCATGGATTATGTGCAAGCAAGAGGATGCCCCCGATCCCGTTATTGACACTTGCAATGGTTTCTACTGTGAGAATTTCAAGCCAAATAAGGACTATAAGCCCAAAATGTGGACAGAAGTCTGGACTGGTTGGTATACAGAATTCGGTGGGGCAGTTCCCACTAGACCTGCAGAAGATGTGGCATTTTCAGTTGCTAGGTTCATACAAAGCGGCGGTTCGTTTTTGAACTATTACATGTACCACGGAGGAACGAATTTTGGCCGAACAGCCGGAGGTCCCTTCATGGCCACTAGCTATGACTACGACGCCCCCTTAGACGAATATGGACTACCCCGGGAACCAAAGTGGGGACATTTGAGAGATCTGCACAAAGCCATTAAATCATGTGAGTCTGCTTTAGTGTCCGTTGATCCTTCAGTGACTAAACTCGGAAGTAATCAAGAGGCTCATGTATTCAAATCAGAGTCGGATTGCGCTGCATTCCTCGCAAATTATGACGCAAAATACTCTGTTAAAGTGAGCTTTGGAGGCGGGCAGTATGACCTGCCGCCATGGTCCATCAGCATTCTTCCGGACTGCAAAACCGAAGTTTACAACACTGCAAAGGTTGGTTCGCAAAGCTCGCAAGTTCAGATGACACCAGTACATAGTGGATTTCCTTGGCAGTCATTCATCGAAGAAACCACCTCTTCTGATGAGACCGATACAACTACATTGGACGGGTTGTATGAGCAAATAAATATCACTAGGGATACTACAGACTACTTGTGGTACATGACAGATATCACAATAGGTTCTGATGAAGCGTTTCTAAAGAACGGAAAGTCCCCACTTCTTACGATCTTTTCAGCAGGTCATGCCTTGAATGTTTTCATCAATGGTCAGCTATCAGGAACCGTGTATGGGTCATTGGAGAATCCTAAATTATCATTCAGTCAAAACGTGAACCTGAGATCTGGCATCAACAAGCTTGCATTGCTTAGCATTTCCGTTGGTCTGCCGAATGTTGGTACTCACTTTGAGACATGGAACGCGGGAGTTCTAGGCCCGATCACGTTGAAGGGTCTGAATTCAGGAACATGGGACATGTCAGGGTGGAAATGGACGTACAAGACTGGTCTGAAAGGTGAAGCTTTAGGCCTCCATACTGTTACTGGGAGTTCTTCTGTTGAATGGGTAGAAGGACCATCGATGGCTGAAAAACAACCCCTTACATGGTACAAGGCTACTTTTAATGCACCACCAGGTGATGCTCCATTAGCTTTAGATATGGGAAGCATGGGAAAAGGTCAGATATGGATAAATGGACAGAGCGTGGGACGCCACTGGCCCGGGTACATTGCACGCGGCAGCTGTGGCGATTGTTCTTATGCCGGAACTTATGATGATAAGAAATGCAGAACTCATTGTGGCGAGCCCTCTCAGAGATGGTACCACATTCCTCGATCATGGTTGACCCCGACTGGGAATCTTTTGGTGGTGTTCGAAGAATGGGGTGGTGATCCGTCAAGGATTTCGTTGGTTGAAAGAGGTACAGCCCTCGACGCGAAGAAGCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

732

Amino Acids

80.9

Weight (kDa)

5.69

Isoelectric Point (pI)

36.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	33 - 337	8.6e-120	Glycosyl hydrolases family 35
GHD	PF17834	360 - 415	1.1e-22	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	467 - 534	7.8e-07	Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd	PF21467	616 - 702	4.4e-25	Beta-galactosidase, galactose-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002889)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56870
fragaria_vesca	FvH4_1g07401 FvH4_1g07410 FvH4_1g07410
malus_domestica	MD02G1079200.v1.1 MD08G1139000.v1.1 MD13G1126600.v1.1 MD15G1206800.v1.1
prunus_persica	Prupe.7G210000_v2.0.a1 Prupe.7G210000_v2.0.a1
pyrus_communis	pycom02g06300 pycom15g18320
rosa_chinensis	RchiOBHm_Chr2g0093301 RchiOBHm_Chr2g0093311 RchiOBHm_Chr3g0459401 RchiOBHm_Chr3g0459561
rosa_laevigata	RLG00000016388 RLG00000016389 RLG00000025083
rosa_multiflora	Rmu_sc0002089.1_g000005
rosa_roxburghii	Rroxscaffold_2G00148200 Rroxscaffold_2G00148210 Rroxscaffold_5G00345960
rosa_rugosa	Rorug02G0034700.1 Rorug03G0036300
rosa_samantha	Rh2AG080700 Rh2AG080800 Rh2CG084000 Rh2DG079700 Rh3AG094200 Rh3BG097100 Rh3CG098400 Rh3DG097900 Rh3DG098900
rosa_wichuraiana	Rw2G006600 Rw2G006610 Rw3G008110 Rw3G008200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	811, 1188, 1478
Acc65I	GGTACC	1 cut(s)	2067
AccB1I	GGYRCC	1 cut(s)	2067
AccB7I	CCANNNNNTGG	1 cut(s)	744
AccI	GTMKAC	1 cut(s)	770
AccII	CGCG	3 cut(s)	1670, 1983, 2183
AccIII	TCCGGA	2 cut(s)	336, 1207
AciI	CCGC	7 cut(s)	443, 846, 849, 1167, 1184, 1670, 1983
AclWI	GGATC	3 cut(s)	665, 1040, 2132
AcoI	YGGCCR	4 cut(s)	170, 238, 895, 921
AcsI	RAATTY	7 cut(s)	12, 455, 545, 709, 776, 889, 1705
AcuI	CTGAAG	2 cut(s)	51, 1035
AcyI	GRCGYC	2 cut(s)	942, 1957
AdeI	CACNNNGTG	1 cut(s)	1883
AfaI	GTAC	9 cut(s)	875, 1281, 1409, 1648, 1745, 1856, 1973, 2069, 2170
AfiI	CCNNNNNNNGG	8 cut(s)	168, 254, 744, 983, 1539, 1643, 1851, 2100
AflIII	ACRYGT	2 cut(s)	870, 1722
AjnI	CCWGG	3 cut(s)	403, 577, 1879
AjuI	GAANNNNNNNTTGG	4 cut(s)	531, 552, 563, 584
AloI	GAACNNNNNNTCC	2 cut(s)	1445, 1477
Alw21I	GWGCWC	1 cut(s)	577
Alw26I	GTCTC	3 cut(s)	338, 1328, 1652
Alw44I	GTGCAC	1 cut(s)	573
AlwI	GGATC	3 cut(s)	665, 1040, 2132
AlwNI	CAGNNNCTG	1 cut(s)	74
Ama87I	CYCGRG	3 cut(s)	161, 969, 1967
Aor13HI	TCCGGA	2 cut(s)	336, 1207
ApaLI	GTGCAC	1 cut(s)	573
ApeKI	GCWGC	4 cut(s)	62, 602, 1112, 1986
ApoI	RAATTY	7 cut(s)	12, 455, 545, 709, 776, 889, 1705
AseI	ATTAAT	1 cut(s)	105
Asp700I	GAANNNNTTC	2 cut(s)	1443, 1505
Asp718I	GGTACC	1 cut(s)	2067
AspLEI	GCGC	2 cut(s)	359, 1112
AspS9I	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1820, 1965
AsuC2I	CCSGG	5 cut(s)	255, 970, 971, 1968, 1969
AsuHPI	GGTGA	3 cut(s)	1775, 1895, 2147
AsuII	TTCGAA	2 cut(s)	418, 2121
AvaI	CYCGRG	3 cut(s)	161, 969, 1967
AvaII	GGWCC	5 cut(s)	515, 551, 911, 1191, 1820
BaeGI	GKGCMC	1 cut(s)	577
BalI	TGGCCA	2 cut(s)	240, 923
BanI	GGYRCC	1 cut(s)	2067
BanII	GRGCYC	1 cut(s)	2055
Bbv12I	GWGCWC	1 cut(s)	577
BbvI	GCAGC	4 cut(s)	74, 614, 1099, 1998
BccI	CCATC	6 cut(s)	191, 604, 1202, 1822, 1831, 2058
BciT130I	CCWGG	3 cut(s)	405, 579, 1881
BciVI	GTATCC	1 cut(s)	1381
BcnI	CCSGG	5 cut(s)	255, 970, 971, 1968, 1969
BcoDI	GTCTC	3 cut(s)	338, 1328, 1652
BfaI	CTAG	7 cut(s)	623, 798, 831, 927, 1383, 1679, 2194
BfmI	CTRYAG	4 cut(s)	60, 615, 804, 1392
BfuAI	ACCTGC	3 cut(s)	811, 1188, 1478
BfuI	GTATCC	1 cut(s)	1381
BglII	AGATCT	2 cut(s)	997, 1589
BisI	GCNGC	8 cut(s)	63, 444, 603, 847, 1113, 1184, 1984, 1987
BlpI	GCTNAGC	1 cut(s)	1616
BlsI	GCNGC	8 cut(s)	64, 445, 604, 848, 1114, 1185, 1985, 1988
Bme1390I	CCNGG	8 cut(s)	255, 405, 579, 970, 971, 1881, 1968, 1969
Bme18I	GGWCC	5 cut(s)	515, 551, 911, 1191, 1820
BmeT110I	CYCGRG	3 cut(s)	161, 969, 1967
BmgT120I	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1820, 1965
BmiI	GGNNCC	5 cut(s)	136, 913, 975, 1531, 2069
BmrFI	CCNGG	8 cut(s)	255, 405, 579, 970, 971, 1881, 1968, 1969
BmrI	ACTGGG	3 cut(s)	377, 1800, 2109
BmsI	GCATC	3 cut(s)	652, 1605, 1876
BmuI	ACTGGG	3 cut(s)	377, 1800, 2109
BoxI	GACNNNNGTC	1 cut(s)	752
BpmI	CTGGAG	1 cut(s)	651
Bpu1102I	GCTNAGC	1 cut(s)	1616
Bpu14I	TTCGAA	2 cut(s)	418, 2121
BpuEI	CTTGAG	1 cut(s)	492
BpuMI	CCSGG	5 cut(s)	255, 970, 971, 1968, 1969
Bsa29I	ATCGAT	1 cut(s)	1826
BsaHI	GRCGYC	2 cut(s)	942, 1957
BsaI	GGTCTC	1 cut(s)	1328
BsaJI	CCNNGG	8 cut(s)	403, 638, 877, 968, 969, 1187, 1295, 1967
BsaWI	WCCGGW	2 cut(s)	336, 1207
Bsc4I	CCNNNNNNNGG	8 cut(s)	168, 254, 744, 983, 1539, 1643, 1851, 2100
Bse1I	ACTGG	8 cut(s)	383, 634, 766, 1277, 1756, 1795, 1967, 2104
Bse3DI	GCAATG	4 cut(s)	453, 697, 1610, 1974
BseAI	TCCGGA	2 cut(s)	336, 1207
BseBI	CCWGG	3 cut(s)	405, 579, 1881
BseCI	ATCGAT	1 cut(s)	1826
BseDI	CCNNGG	8 cut(s)	403, 638, 877, 968, 969, 1187, 1295, 1967
BseGI	GGATG	2 cut(s)	214, 667
BseLI	CCNNNNNNNGG	8 cut(s)	168, 254, 744, 983, 1539, 1643, 1851, 2100
BseMI	GCAATG	4 cut(s)	453, 697, 1610, 1974
BseMII	CTCAG	4 cut(s)	545, 620, 1577, 2072
BseNI	ACTGG	8 cut(s)	383, 634, 766, 1277, 1756, 1795, 1967, 2104
BseSI	GKGCMC	1 cut(s)	577
BseXI	GCAGC	4 cut(s)	74, 614, 1099, 1998
BsgI	GTGCAG	1 cut(s)	986
Bsh1236I	CGCG	3 cut(s)	1670, 1983, 2183
BshNI	GGYRCC	1 cut(s)	2067
BshVI	ATCGAT	1 cut(s)	1826
BsiHKAI	GWGCWC	1 cut(s)	577
BsiHKCI	CYCGRG	3 cut(s)	161, 969, 1967
BsiSI	CCGG	8 cut(s)	173, 254, 337, 906, 970, 1208, 1968, 2010
BslFI	GGGAC	6 cut(s)	386, 897, 999, 1447, 1733, 1968
BslI	CCNNNNNNNGG	8 cut(s)	168, 254, 744, 983, 1539, 1643, 1851, 2100
BsmAI	GTCTC	3 cut(s)	338, 1328, 1652
BsmFI	GGGAC	6 cut(s)	386, 897, 999, 1447, 1733, 1968
BsmI	GAATGC	3 cut(s)	30, 1115, 1200
Bso31I	GGTCTC	1 cut(s)	1328
BsoBI	CYCGRG	3 cut(s)	161, 969, 1967
Bsp119I	TTCGAA	2 cut(s)	418, 2121
Bsp1286I	GDGCHC	2 cut(s)	577, 2055
Bsp13I	TCCGGA	2 cut(s)	336, 1207
Bsp143I	GATC	8 cut(s)	670, 997, 1045, 1476, 1589, 1686, 2081, 2137
Bsp1720I	GCTNAGC	1 cut(s)	1616
Bsp19I	CCATGG	2 cut(s)	638, 1187
BspACI	CCGC	7 cut(s)	443, 846, 849, 1167, 1184, 1670, 1983
BspCNI	CTCAG	4 cut(s)	544, 619, 1578, 2071
BspDI	ATCGAT	1 cut(s)	1826
BspEI	TCCGGA	2 cut(s)	336, 1207
BspFNI	CGCG	3 cut(s)	1670, 1983, 2183
BspLI	GGNNCC	5 cut(s)	136, 913, 975, 1531, 2069
BspMAI	CTGCAG	2 cut(s)	64, 808
BspMI	ACCTGC	3 cut(s)	811, 1188, 1478
BspPI	GGATC	3 cut(s)	665, 1040, 2132
BspT104I	TTCGAA	2 cut(s)	418, 2121
BspT107I	GGYRCC	1 cut(s)	2067
BspTNI	GGTCTC	1 cut(s)	1328
BsrDI	GCAATG	4 cut(s)	453, 697, 1610, 1974
BsrI	ACTGG	8 cut(s)	383, 634, 766, 1277, 1756, 1795, 1967, 2104
BssECI	CCNNGG	8 cut(s)	403, 638, 877, 968, 969, 1187, 1295, 1967
BssMI	GATC	8 cut(s)	670, 997, 1045, 1476, 1589, 1686, 2081, 2137
BssNAI	GTATAC	1 cut(s)	771
BssNI	GRCGYC	2 cut(s)	942, 1957
BssT1I	CCWWGG	3 cut(s)	638, 1187, 1295
Bst1107I	GTATAC	1 cut(s)	771
Bst2UI	CCWGG	3 cut(s)	405, 579, 1881
Bst4CI	ACNGT	3 cut(s)	704, 1534, 1786
Bst6I	CTCTTC	1 cut(s)	1330
BstACI	GRCGYC	2 cut(s)	942, 1957
BstAPI	GCANNNNNTGC	1 cut(s)	1493
BstBI	TTCGAA	2 cut(s)	418, 2121
BstC8I	GCNNGC	7 cut(s)	319, 653, 1169, 1259, 1608, 1981, 2051
BstDEI	CTNAG	6 cut(s)	531, 606, 949, 1586, 1616, 2058
BstDSI	CCRYGG	3 cut(s)	638, 877, 1187
BstF5I	GGATG	2 cut(s)	214, 667
BstFNI	CGCG	3 cut(s)	1670, 1983, 2183
BstHHI	GCGC	2 cut(s)	359, 1112
BstKTI	GATC	8 cut(s)	673, 1000, 1048, 1479, 1592, 1689, 2084, 2140
BstMAI	GTCTC	3 cut(s)	338, 1328, 1652
BstMBI	GATC	8 cut(s)	670, 997, 1045, 1476, 1589, 1686, 2081, 2137
BstNI	CCWGG	3 cut(s)	405, 579, 1881
BstNSI	RCATGY	2 cut(s)	874, 1726
BstPAI	GACNNNNGTC	1 cut(s)	752
BstSCI	CCNGG	8 cut(s)	253, 403, 577, 968, 969, 1879, 1966, 1967
BstSFI	CTRYAG	4 cut(s)	60, 615, 804, 1392
BstSLI	GKGCMC	1 cut(s)	577
BstUI	CGCG	3 cut(s)	1670, 1983, 2183
BstV1I	GCAGC	4 cut(s)	74, 614, 1099, 1998
BstX2I	RGATCY	2 cut(s)	997, 1589
BstYI	RGATCY	2 cut(s)	997, 1589
BstZ17I	GTATAC	1 cut(s)	771
Bsu15I	ATCGAT	1 cut(s)	1826
BsuI	GTATCC	1 cut(s)	1381
BsuTUI	ATCGAT	1 cut(s)	1826
BtgI	CCRYGG	3 cut(s)	638, 877, 1187
BtsCI	GGATG	2 cut(s)	214, 667
BtsI	GCAGTG	1 cut(s)	1233
BtsIMutI	CAGTG	4 cut(s)	627, 1059, 1233, 1960
BveI	ACCTGC	3 cut(s)	811, 1188, 1478
Cac8I	GCNNGC	7 cut(s)	319, 653, 1169, 1259, 1608, 1981, 2051
CaiI	CAGNNNCTG	1 cut(s)	74
CfoI	GCGC	2 cut(s)	359, 1112
Cfr13I	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1820, 1965
Cfr9I	CCCGGG	2 cut(s)	969, 1967
ClaI	ATCGAT	1 cut(s)	1826
CseI	GACGC	4 cut(s)	950, 1142, 1965, 2189
CsiI	ACCWGGT	1 cut(s)	1879
Csp6I	GTAC	9 cut(s)	874, 1280, 1408, 1647, 1744, 1855, 1972, 2068, 2169
CspCI	CAANNNNNGTGG	2 cut(s)	5, 40
CviQI	GTAC	9 cut(s)	874, 1280, 1408, 1647, 1744, 1855, 1972, 2068, 2169
DdeI	CTNAG	6 cut(s)	531, 606, 949, 1586, 1616, 2058
DpnI	GATC	8 cut(s)	672, 999, 1047, 1478, 1591, 1688, 2083, 2139
DpnII	GATC	8 cut(s)	670, 997, 1045, 1476, 1589, 1686, 2081, 2137
DraIII	CACNNNGTG	1 cut(s)	1883
EaeI	YGGCCR	4 cut(s)	170, 238, 895, 921
Eam1104I	CTCTTC	1 cut(s)	1330
EarI	CTCTTC	1 cut(s)	1330
Eco130I	CCWWGG	3 cut(s)	638, 1187, 1295
Eco147I	AGGCCT	3 cut(s)	321, 621, 1776
Eco24I	GRGCYC	1 cut(s)	2055
Eco31I	GGTCTC	1 cut(s)	1328
Eco32I	GATATC	1 cut(s)	1420
Eco47I	GGWCC	5 cut(s)	515, 551, 911, 1191, 1820
Eco57I	CTGAAG	2 cut(s)	51, 1035
Eco88I	CYCGRG	3 cut(s)	161, 969, 1967
EcoO109I	RGGNCCY	2 cut(s)	515, 911
EcoRI	GAATTC	3 cut(s)	12, 776, 1705
EcoRII	CCWGG	3 cut(s)	403, 577, 1879
EcoRV	GATATC	1 cut(s)	1420
EcoT14I	CCWWGG	3 cut(s)	638, 1187, 1295
EcoT38I	GRGCYC	1 cut(s)	2055
ErhI	CCWWGG	3 cut(s)	638, 1187, 1295
FaqI	GGGAC	6 cut(s)	386, 897, 999, 1447, 1733, 1968
FauI	CCCGC	2 cut(s)	1160, 1663
FblI	GTMKAC	1 cut(s)	770
Fnu4HI	GCNGC	8 cut(s)	63, 444, 603, 847, 1113, 1184, 1984, 1987
FokI	GGATG	2 cut(s)	221, 674
FriOI	GRGCYC	1 cut(s)	2055
Fsp4HI	GCNGC	8 cut(s)	63, 444, 603, 847, 1113, 1184, 1984, 1987
FspBI	CTAG	7 cut(s)	623, 798, 831, 927, 1383, 1679, 2194
GlaI	GCGC	2 cut(s)	358, 1111
GluI	GCNGC	8 cut(s)	63, 444, 603, 847, 1113, 1184, 1984, 1987
GsuI	CTGGAG	1 cut(s)	651
HapII	CCGG	8 cut(s)	173, 254, 337, 906, 970, 1208, 1968, 2010
HgaI	GACGC	4 cut(s)	950, 1142, 1965, 2189
HhaI	GCGC	2 cut(s)	359, 1112
Hin1I	GRCGYC	2 cut(s)	942, 1957
Hin6I	GCGC	2 cut(s)	357, 1110
HinP1I	GCGC	2 cut(s)	357, 1110
HincII	GTYRAC	1 cut(s)	2091
HindII	GTYRAC	1 cut(s)	2091
HindIII	AAGCTT	3 cut(s)	585, 1604, 1767
HinfI	GANTC	6 cut(s)	378, 408, 1025, 1100, 1552, 2104
HpaII	CCGG	8 cut(s)	173, 254, 337, 906, 970, 1208, 1968, 2010
HphI	GGTGA	3 cut(s)	1775, 1895, 2147
Hpy166II	GTNNAC	6 cut(s)	575, 747, 771, 1228, 1582, 2091
Hpy188III	TCNNGA	7 cut(s)	161, 337, 757, 1076, 1208, 1527, 1710
Hpy8I	GTNNAC	6 cut(s)	575, 747, 771, 1228, 1582, 2091
Hpy99I	CGWCG	2 cut(s)	944, 2183
HpyAV	CCTTC	7 cut(s)	181, 258, 478, 925, 1059, 1690, 1811
HpyCH4III	ACNGT	3 cut(s)	704, 1534, 1786
HpyCH4IV	ACGT	3 cut(s)	1578, 1691, 1742
HpyF3I	CTNAG	6 cut(s)	531, 606, 949, 1586, 1616, 2058
HpySE526I	ACGT	3 cut(s)	1578, 1691, 1742
Hsp92I	GRCGYC	2 cut(s)	942, 1957
HspAI	GCGC	2 cut(s)	357, 1110
Kpn2I	TCCGGA	2 cut(s)	336, 1207
KpnI	GGTACC	1 cut(s)	2071
Kzo9I	GATC	8 cut(s)	670, 997, 1045, 1476, 1589, 1686, 2081, 2137
LmnI	GCTCC	3 cut(s)	27, 140, 1894
Lsp1109I	GCAGC	4 cut(s)	74, 614, 1099, 1998
LweI	GCATC	3 cut(s)	652, 1605, 1876
MabI	ACCWGGT	1 cut(s)	1879
MaeI	CTAG	7 cut(s)	623, 798, 831, 927, 1383, 1679, 2194
MaeII	ACGT	3 cut(s)	1578, 1691, 1742
MaeIII	GTNAC	2 cut(s)	1054, 1786
MalI	GATC	8 cut(s)	672, 999, 1047, 1478, 1591, 1688, 2083, 2139
MboI	GATC	8 cut(s)	670, 997, 1045, 1476, 1589, 1686, 2081, 2137
MboII	GAAGA	7 cut(s)	478, 821, 1196, 1317, 1325, 1791, 2135
MflI	RGATCY	2 cut(s)	997, 1589
MhlI	GDGCHC	2 cut(s)	577, 2055
MlsI	TGGCCA	2 cut(s)	240, 923
MluNI	TGGCCA	2 cut(s)	240, 923
MlyI	GAGTC	2 cut(s)	1034, 1109
MmeI	TCCRAC	1 cut(s)	1083
Mox20I	TGGCCA	2 cut(s)	240, 923
MroI	TCCGGA	2 cut(s)	336, 1207
MroXI	GAANNNNTTC	2 cut(s)	1443, 1505
MscI	TGGCCA	2 cut(s)	240, 923
MseI	TTAA	6 cut(s)	105, 125, 179, 1014, 1149, 1869
Msp20I	TGGCCA	2 cut(s)	240, 923
MspA1I	CMGCKG	1 cut(s)	1989
MspI	CCGG	8 cut(s)	173, 254, 337, 906, 970, 1208, 1968, 2010
MspR9I	CCNGG	8 cut(s)	255, 405, 579, 970, 971, 1881, 1968, 1969
Mva1269I	GAATGC	3 cut(s)	30, 1115, 1200
MvaI	CCWGG	3 cut(s)	405, 579, 1881
MvnI	CGCG	3 cut(s)	1670, 1983, 2183
NciI	CCSGG	5 cut(s)	255, 970, 971, 1968, 1969
NcoI	CCATGG	2 cut(s)	638, 1187
NdeII	GATC	8 cut(s)	670, 997, 1045, 1476, 1589, 1686, 2081, 2137
NlaIV	GGNNCC	5 cut(s)	136, 913, 975, 1531, 2069
NmuCI	GTSAC	1 cut(s)	1054
NspI	RCATGY	2 cut(s)	874, 1726
NspV	TTCGAA	2 cut(s)	418, 2121
PceI	AGGCCT	3 cut(s)	321, 621, 1776
PciI	ACATGT	2 cut(s)	870, 1722
PctI	GAATGC	3 cut(s)	30, 1115, 1200
PdmI	GAANNNNTTC	2 cut(s)	1443, 1505
PfeI	GAWTC	4 cut(s)	378, 408, 1552, 2104
PflMI	CCANNNNNTGG	1 cut(s)	744
PfoI	TCCNGGA	1 cut(s)	253
PkrI	GCNGC	8 cut(s)	64, 445, 604, 848, 1114, 1185, 1985, 1988
PleI	GAGTC	2 cut(s)	1033, 1108
PpsI	GAGTC	2 cut(s)	1033, 1108
PpuMI	RGGWCCY	2 cut(s)	515, 911
PscI	ACATGT	2 cut(s)	870, 1722
PshAI	GACNNNNGTC	1 cut(s)	752
PshBI	ATTAAT	1 cut(s)	105
Psp5II	RGGWCCY	2 cut(s)	515, 911
Psp6I	CCWGG	3 cut(s)	403, 577, 1879
PspGI	CCWGG	3 cut(s)	403, 577, 1879
PspN4I	GGNNCC	5 cut(s)	136, 913, 975, 1531, 2069
PspPI	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1820, 1965
PspPPI	RGGWCCY	2 cut(s)	515, 911
PstI	CTGCAG	2 cut(s)	64, 808
PstNI	CAGNNNCTG	1 cut(s)	74
PsuI	RGATCY	2 cut(s)	997, 1589
PvuII	CAGCTG	1 cut(s)	1989
RsaI	GTAC	9 cut(s)	875, 1281, 1409, 1648, 1745, 1856, 1973, 2069, 2170
RsaNI	GTAC	9 cut(s)	874, 1280, 1408, 1647, 1744, 1855, 1972, 2068, 2169
SaqAI	TTAA	6 cut(s)	105, 125, 179, 1014, 1149, 1869
SatI	GCNGC	8 cut(s)	63, 444, 603, 847, 1113, 1184, 1984, 1987
Sau3AI	GATC	8 cut(s)	670, 997, 1045, 1476, 1589, 1686, 2081, 2137
Sau96I	GGNCC	8 cut(s)	344, 515, 551, 911, 1191, 1682, 1820, 1965
SchI	GAGTC	2 cut(s)	1034, 1109
ScrFI	CCNGG	8 cut(s)	255, 405, 579, 970, 971, 1881, 1968, 1969
SduI	GDGCHC	2 cut(s)	577, 2055
SexAI	ACCWGGT	1 cut(s)	1879
SfaNI	GCATC	3 cut(s)	652, 1605, 1876
SfcI	CTRYAG	4 cut(s)	60, 615, 804, 1392
SfuI	TTCGAA	2 cut(s)	418, 2121
SinI	GGWCC	5 cut(s)	515, 551, 911, 1191, 1820
SmaI	CCCGGG	2 cut(s)	971, 1969
SmlI	CTYRAG	1 cut(s)	507
SmoI	CTYRAG	1 cut(s)	507
SseBI	AGGCCT	3 cut(s)	321, 621, 1776
SsiI	CCGC	7 cut(s)	443, 846, 849, 1167, 1184, 1670, 1983
SspMI	CTAG	7 cut(s)	623, 798, 831, 927, 1383, 1679, 2194
StuI	AGGCCT	3 cut(s)	321, 621, 1776
StyD4I	CCNGG	8 cut(s)	253, 403, 577, 968, 969, 1879, 1966, 1967
StyI	CCWWGG	3 cut(s)	638, 1187, 1295
TaaI	ACNGT	3 cut(s)	704, 1534, 1786
TaiI	ACGT	3 cut(s)	1581, 1694, 1745
TaqI	TCGA	7 cut(s)	270, 418, 1311, 1826, 2080, 2121, 2178
TaqII	GACCGA	1 cut(s)	1352
TatI	WGTACW	1 cut(s)	1279
TauI	GCSGC	4 cut(s)	446, 849, 1186, 1986
TfiI	GAWTC	4 cut(s)	378, 408, 1552, 2104
Tru1I	TTAA	6 cut(s)	105, 125, 179, 1014, 1149, 1869
Tru9I	TTAA	6 cut(s)	105, 125, 179, 1014, 1149, 1869
TscAI	CASTG	4 cut(s)	634, 1059, 1240, 1967
TseFI	GTSAC	1 cut(s)	1054
TseI	GCWGC	4 cut(s)	62, 602, 1112, 1986
Tsp45I	GTSAC	1 cut(s)	1054
TspDTI	ATGAA	8 cut(s)	258, 497, 558, 826, 907, 1297, 1452, 1498
TspGWI	ACGGA	6 cut(s)	437, 894, 1030, 1470, 1615, 2130
TspMI	CCCGGG	2 cut(s)	969, 1967
TspRI	CASTG	4 cut(s)	634, 1059, 1240, 1967
Van91I	CCANNNNNTGG	1 cut(s)	744
VneI	GTGCAC	1 cut(s)	573
VpaK11BI	GGWCC	5 cut(s)	515, 551, 911, 1191, 1820
VspI	ATTAAT	1 cut(s)	105
XapI	RAATTY	7 cut(s)	12, 455, 545, 709, 776, 889, 1705
XceI	RCATGY	2 cut(s)	874, 1726
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1787
XmaI	CCCGGG	2 cut(s)	969, 1967
XmiI	GTMKAC	1 cut(s)	770
XmnI	GAANNNNTTC	2 cut(s)	1443, 1505
XspI	CTAG	7 cut(s)	623, 798, 831, 927, 1383, 1679, 2194

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.