Rroxscaffold_5G00345960

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002889
Physical Location & Seq
Reverse (-)
16556538 .. 16557007
470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00345960.1

Sequence Viewer

Length: 294 bp
ATGACACCAGTAAGTCGCCTTTCTTGGCAAGCTTACATCGAAGAAGTTGCCACTTCTATTGATGACAAGGCATTTACATTAGTTGGACTTCGTGAGCAACTAAGTCTCACTAGGGATGCTATAGACTACTTGTGGTACATGACAGATATCACAGTAGGTCCTCATGAACAATTTTTAAGAACTGGAAAGTACCCCACTCTCAAAGTTGATTCAGCTGGGCATGCCTTGCATGTTTTCATCAATGGTCAACGATCAGGATCTGCTTATGGATCATTGCAGTCCCCCAAATTATAA

Protein Analysis

97

Amino Acids

10.86

Weight (kDa)

6.25

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BetaGal_gal-bd PF21467 43 - 91 3.5e-07 Beta-galactosidase, galactose-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 292
AclWI GGATC 2 cut(s) 265, 277
AfaI GTAC 2 cut(s) 137, 191
AluBI AGCT 2 cut(s) 32, 215
AluI AGCT 2 cut(s) 32, 215
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 2 cut(s) 265, 277
AlwNI CAGNNNCTG 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 158
AvaII GGWCC 1 cut(s) 158
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 1 cut(s) 120
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmsI GCATC 1 cut(s) 106
BsaBI GATNNNNATC 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 8, 187
Bse3DI GCAATG 1 cut(s) 272
Bse8I GATNNNNATC 1 cut(s) 256
BseGI GGATG 1 cut(s) 121
BseJI GATNNNNATC 1 cut(s) 256
BseMI GCAATG 1 cut(s) 272
BseNI ACTGG 2 cut(s) 8, 187
BseYI CCCAGC 1 cut(s) 215
BslFI GGGAC 1 cut(s) 265
BsmAI GTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 265
Bsp143I GATC 3 cut(s) 251, 257, 269
BspHI TCATGA 1 cut(s) 163
BspPI GGATC 2 cut(s) 265, 277
BsrDI GCAATG 1 cut(s) 272
BsrI ACTGG 2 cut(s) 8, 187
BssMI GATC 3 cut(s) 251, 257, 269
Bst4CI ACNGT 1 cut(s) 154
BstAPI GCANNNNNTGC 1 cut(s) 226
BstC8I GCNNGC 2 cut(s) 30, 222
BstDEI CTNAG 1 cut(s) 101
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 3 cut(s) 254, 260, 272
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 3 cut(s) 251, 257, 269
BstMWI GCNNNNNNNGC 2 cut(s) 221, 226
BstNSI RCATGY 2 cut(s) 224, 233
BstSFI CTRYAG 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 257
BstYI RGATCY 1 cut(s) 257
BtsCI GGATG 1 cut(s) 121
Cac8I GCNNGC 2 cut(s) 30, 222
CaiI CAGNNNCTG 1 cut(s) 260
CciI TCATGA 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 158
Csp6I GTAC 2 cut(s) 136, 190
CspCI CAANNNNNGTGG 2 cut(s) 40, 75
CviAII CATG 4 cut(s) 139, 164, 221, 230
CviJI RGCY 2 cut(s) 32, 215
CviKI_1 RGCY 2 cut(s) 32, 215
CviQI GTAC 2 cut(s) 136, 190
DdeI CTNAG 1 cut(s) 101
DpnI GATC 3 cut(s) 253, 259, 271
DpnII GATC 3 cut(s) 251, 257, 269
Eco32I GATATC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 158
EcoO109I RGGNCCY 1 cut(s) 158
EcoRV GATATC 1 cut(s) 148
FaeI CATG 4 cut(s) 142, 167, 224, 233
FaiI YATR 7 cut(s) 122, 140, 165, 222, 231, 267, 292
FaqI GGGAC 1 cut(s) 265
FatI CATG 4 cut(s) 138, 163, 220, 229
FokI GGATG 1 cut(s) 128
FspBI CTAG 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 219
Hin1II CATG 4 cut(s) 142, 167, 224, 233
HincII GTYRAC 1 cut(s) 248
HindII GTYRAC 1 cut(s) 248
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 1 cut(s) 209
Hpy166II GTNNAC 1 cut(s) 248
Hpy188III TCNNGA 3 cut(s) 92, 164, 255
Hpy8I GTNNAC 1 cut(s) 248
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 229, 277
HpyF10VI GCNNNNNNNGC 2 cut(s) 221, 226
HpyF3I CTNAG 1 cut(s) 101
Hsp92II CATG 4 cut(s) 142, 167, 224, 233
Kzo9I GATC 3 cut(s) 251, 257, 269
LpnPI CCDG 4 cut(s) 21, 168, 201, 240
LweI GCATC 1 cut(s) 106
MaeI CTAG 1 cut(s) 111
MalI GATC 3 cut(s) 253, 259, 271
MboI GATC 3 cut(s) 251, 257, 269
MboII GAAGA 1 cut(s) 53
MflI RGATCY 1 cut(s) 257
MluCI AATT 2 cut(s) 170, 287
MmeI TCCRAC 1 cut(s) 64
MnlI CCTC 1 cut(s) 171
MseI TTAA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 221, 226
NdeII GATC 3 cut(s) 251, 257, 269
NlaIII CATG 4 cut(s) 142, 167, 224, 233
NspI RCATGY 2 cut(s) 224, 233
PaeI GCATGC 1 cut(s) 224
PagI TCATGA 1 cut(s) 163
PfeI GAWTC 1 cut(s) 209
PpuMI RGGWCCY 1 cut(s) 158
PsiI TTATAA 1 cut(s) 292
Psp5II RGGWCCY 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 215
PspPI GGNCC 1 cut(s) 158
PspPPI RGGWCCY 1 cut(s) 158
PstNI CAGNNNCTG 1 cut(s) 260
PsuI RGATCY 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 2 cut(s) 137, 191
RsaNI GTAC 2 cut(s) 136, 190
SaqAI TTAA 1 cut(s) 176
Sau3AI GATC 3 cut(s) 251, 257, 269
Sau96I GGNCC 1 cut(s) 158
SetI ASST 3 cut(s) 34, 160, 217
SfaNI GCATC 1 cut(s) 106
SfcI CTRYAG 1 cut(s) 120
SinI GGWCC 1 cut(s) 158
SphI GCATGC 1 cut(s) 224
Sse9I AATT 2 cut(s) 170, 287
SspMI CTAG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 154
TaqI TCGA 1 cut(s) 39
TasI AATT 2 cut(s) 170, 287
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TspDTI ATGAA 2 cut(s) 180, 226
VpaK11BI GGWCC 1 cut(s) 158
XceI RCATGY 2 cut(s) 224, 233
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.