MD02G1086300.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005421
Physical Location & Seq
Reverse (-)
6766308 .. 6767471
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1086300.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGCTTCTTCTTCACCAACTCTCCCTTCCGACGTTATACGTTCCGAGATTTTAACCCGAATATCCCCGGAAACCCTAGGCAGTTGCAGACTTGTCTCGAAGGAATGGTACCATGCCGCCTATGACTCCTTCAAACTATTTTGTGAGAAAACCAACACAACATATGGCTTCTTCCTTCAGAGCAAGTTCTACCCAAACCGTGTTTGCACCTTCTTACCAGTGGAAGATAACAACATCGACAACTCCAAAACTATTTCGCTTAGGTTCTTACATTTTCCCTACAGTATTGAAGCCGCCGATCGAGGTTTACTCGTCGGCGTGAGCCATAAAAACCATAAGATTCCTCGCTACTTTGTTTGCGCTCCTACTACTCAATACTGGAAACAAATACCAAACCCTAAGACTAGGTATTTTACTCGGGGCACTGCTATTGCCGTGTTGAGGTCGAGTTCGAATTCGAACCCAGTTTTGAGCAGCTGGTTTAAGATTATTCGATTATCGGAGCCCGAGACCAACCCCTCATTTGACAACCACTATATAAGCGAAGTCTTTGATTCAAACACTTGGTCTTGGAAGCAGCTGAACGATTTGATTTTACCTCACGGGGTTTTATTGGGCACCAATTCACCTGTTTCAGCGTGCTGCGCGCTTTACTGGCATCTTACCAACAACCAAATACTGGCTTTCTATCAAGACAAGGAAACCTGGGAAACCTTCGCGCTACCACACCCTATGTGCAACAACGTGCATTGGAAGTACAGTTTAACCGCTTACCAAGGCCGTCTCGCGTTGGTTCGCAAAGAAGGAGGTGATGAGTTCACGGAATACTGGATAATGGAAGACCACCGAGAAAAAATATGGAGTGAGAGGAAGAGACTAAGCACCCGAGGGACTTCTGTTATAGCATTCTGCGACAGTGACGTTGCGCTCATGAAGGAATATGGGAAGATGATATTTTACAATTTTAAAGAGTGCAGAGTATCCAAGCTAGTGGAACTAGAGAGCTGGCATGAGCCGGACTACATTTTCAGTTTTCCGGCCACAAAAAATCCGGGAAATATTGCCGATCATGTCACTCACAACTTAGCTTGGGGCTGGTGTTTCAAGTTCATCCTTTTGTTGTTTGTCATTACTTTGTTTTGGTTTTGGTTATTAGACATTTAA

Protein Analysis

388

Amino Acids

45.14

Weight (kDa)

7.98

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 100 - 325 1.3e-08 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 108
AccB1I GGYRCC 2 cut(s) 108, 617
AccB7I CCANNNNNTGG 1 cut(s) 679
AccII CGCG 3 cut(s) 647, 719, 788
AciI CCGC 3 cut(s) 117, 294, 768
AcoI YGGCCR 1 cut(s) 1038
AcsI RAATTY 1 cut(s) 454
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 2 cut(s) 110, 758
AfiI CCNNNNNNNGG 2 cut(s) 441, 679
AgsI TTSAA 4 cut(s) 133, 290, 558, 1105
AjnI CCWGG 1 cut(s) 704
AjuI GAANNNNNNNTTGG 2 cut(s) 10, 42
AluBI AGCT 5 cut(s) 477, 580, 988, 1005, 1088
AluI AGCT 5 cut(s) 477, 580, 988, 1005, 1088
Alw26I GTCTC 4 cut(s) 100, 503, 788, 868
Ama87I CYCGRG 3 cut(s) 417, 506, 885
AoxI GGCC 2 cut(s) 778, 1038
ApeKI GCWGC 3 cut(s) 474, 577, 642
ApoI RAATTY 1 cut(s) 454
ArsI GACNNNNNNTTYG 2 cut(s) 551, 583
Asp718I GGTACC 1 cut(s) 108
AspA2I CCTAGG 1 cut(s) 76
AspLEI GCGC 5 cut(s) 362, 647, 649, 721, 928
AsuC2I CCSGG 2 cut(s) 68, 1053
AsuHPI GGTGA 3 cut(s) 6, 618, 821
AsuII TTCGAA 2 cut(s) 452, 458
AvaI CYCGRG 3 cut(s) 417, 506, 885
AvrII CCTAGG 1 cut(s) 76
BaeGI GKGCMC 2 cut(s) 425, 620
BanI GGYRCC 2 cut(s) 108, 617
BanII GRGCYC 1 cut(s) 507
BarI GAAGNNNNNNTAC 2 cut(s) 92, 124
BbsI GAAGAC 1 cut(s) 846
BbvI GCAGC 3 cut(s) 486, 589, 629
BceAI ACGGC 2 cut(s) 419, 765
BciT130I CCWGG 1 cut(s) 706
BciVI GTATCC 1 cut(s) 991
BcnI CCSGG 2 cut(s) 68, 1053
BcoDI GTCTC 4 cut(s) 100, 503, 788, 868
BfaI CTAG 4 cut(s) 77, 405, 989, 998
BfmI CTRYAG 1 cut(s) 280
BfuI GTATCC 1 cut(s) 991
BisI GCNGC 5 cut(s) 117, 294, 475, 578, 643
BlnI CCTAGG 1 cut(s) 76
BlsI GCNGC 5 cut(s) 118, 295, 476, 579, 644
Bme1390I CCNGG 3 cut(s) 68, 706, 1053
BmeT110I CYCGRG 3 cut(s) 417, 506, 885
BmiI GGNNCC 3 cut(s) 110, 504, 619
BmrFI CCNGG 3 cut(s) 68, 706, 1053
BmrI ACTGGG 1 cut(s) 458
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 1 cut(s) 458
BpiI GAAGAC 1 cut(s) 846
BplI GAGNNNNNCTC 2 cut(s) 294, 326
Bpu10I CCTNAGC 1 cut(s) 260
Bpu14I TTCGAA 2 cut(s) 452, 458
BpuMI CCSGG 2 cut(s) 68, 1053
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 5 cut(s) 66, 76, 705, 775, 886
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bsc4I CCNNNNNNNGG 2 cut(s) 441, 679
Bse1I ACTGG 6 cut(s) 218, 383, 464, 659, 684, 833
BseBI CCWGG 1 cut(s) 706
BseDI CCNNGG 5 cut(s) 66, 76, 705, 775, 886
BseGI GGATG 1 cut(s) 1110
BseLI CCNNNNNNNGG 2 cut(s) 441, 679
BseNI ACTGG 6 cut(s) 218, 383, 464, 659, 684, 833
BsePI GCGCGC 1 cut(s) 645
BseSI GKGCMC 2 cut(s) 425, 620
BseXI GCAGC 3 cut(s) 486, 589, 629
BsgI GTGCAG 1 cut(s) 994
Bsh1236I CGCG 3 cut(s) 647, 719, 788
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 2 cut(s) 780, 1040
BshNI GGYRCC 2 cut(s) 108, 617
BsiEI CGRYCG 1 cut(s) 301
BsiHKCI CYCGRG 3 cut(s) 417, 506, 885
BsiSI CCGG 4 cut(s) 68, 1016, 1037, 1052
BslFI GGGAC 1 cut(s) 904
BslI CCNNNNNNNGG 2 cut(s) 441, 679
BsmAI GTCTC 4 cut(s) 100, 503, 788, 868
BsmBI CGTCTC 1 cut(s) 788
BsmFI GGGAC 1 cut(s) 904
BsmI GAATGC 1 cut(s) 905
BsnI GGCC 2 cut(s) 780, 1040
Bso31I GGTCTC 1 cut(s) 503
BsoBI CYCGRG 3 cut(s) 417, 506, 885
Bsp119I TTCGAA 2 cut(s) 452, 458
Bsp1286I GDGCHC 3 cut(s) 425, 507, 620
Bsp143I GATC 2 cut(s) 298, 1066
BspACI CCGC 3 cut(s) 117, 294, 768
BspANI GGCC 2 cut(s) 780, 1040
BspFNI CGCG 3 cut(s) 647, 719, 788
BspHI TCATGA 1 cut(s) 930
BspLI GGNNCC 3 cut(s) 110, 504, 619
BspT104I TTCGAA 2 cut(s) 452, 458
BspT107I GGYRCC 2 cut(s) 108, 617
BspTNI GGTCTC 1 cut(s) 503
BsrI ACTGG 6 cut(s) 218, 383, 464, 659, 684, 833
BssECI CCNNGG 5 cut(s) 66, 76, 705, 775, 886
BssHII GCGCGC 1 cut(s) 645
BssMI GATC 2 cut(s) 298, 1066
BssT1I CCWWGG 2 cut(s) 76, 775
Bst2UI CCWGG 1 cut(s) 706
Bst4CI ACNGT 4 cut(s) 200, 284, 761, 917
Bst6I CTCTTC 1 cut(s) 866
BstBI TTCGAA 2 cut(s) 452, 458
BstC8I GCNNGC 3 cut(s) 640, 647, 1007
BstDEI CTNAG 4 cut(s) 260, 399, 878, 1084
BstF5I GGATG 1 cut(s) 1110
BstFNI CGCG 3 cut(s) 647, 719, 788
BstHHI GCGC 5 cut(s) 362, 647, 649, 721, 928
BstKTI GATC 2 cut(s) 301, 1069
BstMAI GTCTC 4 cut(s) 100, 503, 788, 868
BstMBI GATC 2 cut(s) 298, 1066
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 2 cut(s) 644, 655
BstNI CCWGG 1 cut(s) 706
BstSCI CCNGG 3 cut(s) 66, 704, 1051
BstSFI CTRYAG 1 cut(s) 280
BstSLI GKGCMC 2 cut(s) 425, 620
BstUI CGCG 3 cut(s) 647, 719, 788
BstV1I GCAGC 3 cut(s) 486, 589, 629
BstV2I GAAGAC 1 cut(s) 846
BstXI CCANNNNNNTGG 1 cut(s) 991
BsuI GTATCC 1 cut(s) 991
BsuRI GGCC 2 cut(s) 780, 1040
BtsCI GGATG 1 cut(s) 1110
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 3 cut(s) 225, 423, 922
Cac8I GCNNGC 3 cut(s) 640, 647, 1007
CciI TCATGA 1 cut(s) 930
CfoI GCGC 5 cut(s) 362, 647, 649, 721, 928
Csp6I GTAC 2 cut(s) 109, 757
CviAII CATG 4 cut(s) 113, 931, 1010, 1070
CviQI GTAC 2 cut(s) 109, 757
DdeI CTNAG 4 cut(s) 260, 399, 878, 1084
DpnI GATC 2 cut(s) 300, 1068
DpnII GATC 2 cut(s) 298, 1066
DraI TTTAAA 1 cut(s) 967
EaeI YGGCCR 1 cut(s) 1038
Eam1104I CTCTTC 1 cut(s) 866
EarI CTCTTC 1 cut(s) 866
Eco130I CCWWGG 2 cut(s) 76, 775
Eco24I GRGCYC 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 503
Eco57I CTGAAG 1 cut(s) 161
Eco88I CYCGRG 3 cut(s) 417, 506, 885
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 704
EcoT14I CCWWGG 2 cut(s) 76, 775
EcoT38I GRGCYC 1 cut(s) 507
ErhI CCWWGG 2 cut(s) 76, 775
Esp3I CGTCTC 1 cut(s) 788
FaeI CATG 4 cut(s) 116, 934, 1013, 1073
FaqI GGGAC 1 cut(s) 904
FatI CATG 4 cut(s) 112, 930, 1009, 1069
FauNDI CATATG 1 cut(s) 163
Fnu4HI GCNGC 5 cut(s) 117, 294, 475, 578, 643
FokI GGATG 1 cut(s) 1097
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 5 cut(s) 117, 294, 475, 578, 643
FspBI CTAG 4 cut(s) 77, 405, 989, 998
GlaI GCGC 5 cut(s) 361, 646, 648, 720, 927
GluI GCNGC 5 cut(s) 117, 294, 475, 578, 643
HaeIII GGCC 2 cut(s) 780, 1040
HapII CCGG 4 cut(s) 68, 1016, 1037, 1052
HhaI GCGC 5 cut(s) 362, 647, 649, 721, 928
Hin1II CATG 4 cut(s) 116, 934, 1013, 1073
Hin6I GCGC 5 cut(s) 360, 645, 647, 719, 926
HinP1I GCGC 5 cut(s) 360, 645, 647, 719, 926
HinfI GANTC 3 cut(s) 125, 340, 554
HpaII CCGG 4 cut(s) 68, 1016, 1037, 1052
HphI GGTGA 3 cut(s) 6, 618, 821
Hpy166II GTNNAC 2 cut(s) 308, 819
Hpy188I TCNGA 4 cut(s) 31, 46, 180, 502
Hpy188III TCNNGA 3 cut(s) 97, 692, 931
Hpy8I GTNNAC 2 cut(s) 308, 819
Hpy99I CGWCG 2 cut(s) 35, 317
HpyAV CCTTC 8 cut(s) 36, 94, 139, 185, 220, 724, 797, 928
HpyCH4III ACNGT 4 cut(s) 200, 284, 761, 917
HpyCH4IV ACGT 4 cut(s) 33, 40, 744, 921
HpyCH4V TGCA 5 cut(s) 87, 207, 738, 748, 975
HpyF10VI GCNNNNNNNGC 2 cut(s) 644, 655
HpyF3I CTNAG 4 cut(s) 260, 399, 878, 1084
HpySE526I ACGT 4 cut(s) 33, 40, 744, 921
Hsp92II CATG 4 cut(s) 116, 934, 1013, 1073
HspAI GCGC 5 cut(s) 360, 645, 647, 719, 926
KpnI GGTACC 1 cut(s) 112
Kzo9I GATC 2 cut(s) 298, 1066
LmnI GCTCC 2 cut(s) 367, 502
Lsp1109I GCAGC 3 cut(s) 486, 589, 629
LweI GCATC 1 cut(s) 667
MaeI CTAG 4 cut(s) 77, 405, 989, 998
MaeII ACGT 4 cut(s) 33, 40, 744, 921
MaeIII GTNAC 2 cut(s) 917, 1072
MalI GATC 2 cut(s) 300, 1068
MboI GATC 2 cut(s) 298, 1066
MboII GAAGA 6 cut(s) 3, 163, 236, 851, 883, 958
MhlI GDGCHC 3 cut(s) 425, 507, 620
MluCI AATT 3 cut(s) 454, 622, 961
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 8 cut(s) 296, 354, 435, 529, 609, 800, 861, 881
MseI TTAA 5 cut(s) 53, 483, 764, 966, 1162
MspA1I CMGCKG 2 cut(s) 477, 580
MspI CCGG 4 cut(s) 68, 1016, 1037, 1052
MspR9I CCNGG 3 cut(s) 68, 706, 1053
Mva1269I GAATGC 1 cut(s) 905
MvaI CCWGG 1 cut(s) 706
MvnI CGCG 3 cut(s) 647, 719, 788
MwoI GCNNNNNNNGC 2 cut(s) 644, 655
NciI CCSGG 2 cut(s) 68, 1053
NdeI CATATG 1 cut(s) 163
NdeII GATC 2 cut(s) 298, 1066
NlaIII CATG 4 cut(s) 116, 934, 1013, 1073
NlaIV GGNNCC 3 cut(s) 110, 504, 619
NmuCI GTSAC 2 cut(s) 917, 1072
NspV TTCGAA 2 cut(s) 452, 458
PagI TCATGA 1 cut(s) 930
PauI GCGCGC 1 cut(s) 645
PctI GAATGC 1 cut(s) 905
PfeI GAWTC 2 cut(s) 340, 554
PflMI CCANNNNNTGG 1 cut(s) 679
PfoI TCCNGGA 1 cut(s) 1051
PkrI GCNGC 5 cut(s) 118, 295, 476, 579, 644
Ple19I CGATCG 1 cut(s) 301
PleI GAGTC 1 cut(s) 119
PpsI GAGTC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 704
PspGI CCWGG 1 cut(s) 704
PspN4I GGNNCC 3 cut(s) 110, 504, 619
PteI GCGCGC 1 cut(s) 645
PvuI CGATCG 1 cut(s) 301
PvuII CAGCTG 2 cut(s) 477, 580
RsaI GTAC 2 cut(s) 110, 758
RsaNI GTAC 2 cut(s) 109, 757
SaqAI TTAA 5 cut(s) 53, 483, 764, 966, 1162
SatI GCNGC 5 cut(s) 117, 294, 475, 578, 643
Sau3AI GATC 2 cut(s) 298, 1066
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 3 cut(s) 68, 706, 1053
SduI GDGCHC 3 cut(s) 425, 507, 620
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 1 cut(s) 280
SfuI TTCGAA 2 cut(s) 452, 458
Sse9I AATT 3 cut(s) 454, 622, 961
SsiI CCGC 3 cut(s) 117, 294, 768
SspI AATATT 1 cut(s) 1060
SspMI CTAG 4 cut(s) 77, 405, 989, 998
StyD4I CCNGG 3 cut(s) 66, 704, 1051
StyI CCWWGG 2 cut(s) 76, 775
TaaI ACNGT 4 cut(s) 200, 284, 761, 917
TaiI ACGT 4 cut(s) 36, 43, 747, 924
TaqI TCGA 7 cut(s) 98, 237, 301, 446, 452, 458, 493
TasI AATT 3 cut(s) 454, 622, 961
TatI WGTACW 1 cut(s) 756
TauI GCSGC 2 cut(s) 119, 296
TfiI GAWTC 2 cut(s) 340, 554
Tru1I TTAA 5 cut(s) 53, 483, 764, 966, 1162
Tru9I TTAA 5 cut(s) 53, 483, 764, 966, 1162
TscAI CASTG 3 cut(s) 225, 430, 922
TseFI GTSAC 2 cut(s) 917, 1072
TseI GCWGC 3 cut(s) 474, 577, 642
Tsp45I GTSAC 2 cut(s) 917, 1072
TspDTI ATGAA 2 cut(s) 947, 1099
TspGWI ACGGA 1 cut(s) 836
TspRI CASTG 3 cut(s) 225, 430, 922
Van91I CCANNNNNTGG 1 cut(s) 679
XapI RAATTY 1 cut(s) 454
XmaJI CCTAGG 1 cut(s) 76
XspI CTAG 4 cut(s) 77, 405, 989, 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.