RchiOBHm_Chr2g0094241

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005421
Physical Location & Seq
Reverse (-)
7228460 .. 7229773
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46926

Sequence Viewer

Length: 1314 bp
ATGGCTTCATCACAAACACTTCCTTCTGACCTCTATTTCGAGATTCTGACCCGAGCATCCTTCGATACTGTTGGCCGGTGCAGGCTCGTTTCGAAACAATGGAACCACCTTACATACGATTCAAGTTTCATAAATCAACTATCTCAGAGAACCAAAACAACACACGGGGTTTTCATTCAAAGCTCGATCAGCAGTAATTATATATCCACCTTTGTTCAAGCTGTGGGTAACAACGACGTCTCCAACTCCGAACAGCTGTCCTTAAATTTCCTGCCTCAGCCGGTGAAGATTGAAGCTGCTCATCAGGGTATACTCGTTTGTGTAAGCCAGAAACGTGTTCCTCAATACTATGTCTGCAAGCCAACCACTAGGGAATGGGAGCAGATACACAACCCAAAAACGAGGTATATGACGGTAAAAATTGCCTTAATGGTGTTACGATCAAGCCCGTTAAGGTACAAGATCGTCCGGTTCTCAGAACCAAAGTCTCCATATTCCAAGTACAAGTCGCAGGAACACTGCTACAACCTTAGGTGTGAGGTATATGATTCCAAAGCTCGAGCATGGAAACAGTTGAACAATGTAGCTTTACCCAAAGATGCTTATATCTTGAGGTTTCAACCTGCAGTATCGGCATGCGGTGCACTCCACTGGCTTTTGTCAGACAACCGAGTCGGAATCTTTGCTTTTCATGTAGATGAAGAGAACTGGGAAATATTTGCACCACCGTGCTTGGCAGTCGATAATGTCAATGGGAATCAGGTGGTGGAGTACCAAGGCCGCCTTGCATGGATTTGTGAAGTAGAGGACTCGATGGAGCTATGGGTTATGGAAGACTACAAGAACAAAGTATGGAGTAAAAGACAGATACTAAGGATCAAAGGACTGGATCGTCTTGCTTCTTTTGCAACTCTTTGCAATAATGATGTTGCACTCATGAGAGGATTTTTCAAGATAATATTTTACAAGTTTGGAGATTATAGCTCCAAGATAGCAAATCTAGAGGAAATTTCTCGACCAGATGAGATCTTCATGTTGCAATCAGATTCAGAGCCTACTAGATTGAAGGGTCCATTGAAGTCTTGGGTAATAATAAGATGGTTTCGTTCGATTAAAAAGAAGTGGTCTCCAATAACTAAGTATCGCCTCAGGACTGTTGGGAAATTCTGCAGTACATACGGGACGTATGCCATAGAGTTTGTGTTTCTGTTTTTGGCTTTGAGGACACAATTAATCCAAAGATTGACACACTATCTATATGGCGTAACACATGTATTGCAAACGGTCTTGGAATACTTGTTAGCTGGTGCTTGA

Protein Analysis

437

Amino Acids

50.73

Weight (kDa)

9.35

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 6 - 47 5.4e-06 F-box-like
FBA_1 PF07734 94 - 287 4.2e-14 F-box associated beta propeller domain
FBA_3 PF08268 98 - 291 2.8e-14 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 99 - 240 1.7e-06 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 671, 891
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 310
AciI CCGC 2 cut(s) 639, 781
AclWI GGATC 2 cut(s) 884, 897
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 3 cut(s) 265, 1008, 1163
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 4 cut(s) 458, 503, 773, 1174
AflIII ACRYGT 2 cut(s) 334, 1270
AgsI TTSAA 9 cut(s) 123, 179, 218, 293, 577, 620, 952, 1066, 1078
AleI CACNNNNGTG 1 cut(s) 727
AluBI AGCT 9 cut(s) 183, 221, 256, 296, 557, 587, 820, 984, 1304
AluI AGCT 9 cut(s) 183, 221, 256, 296, 557, 587, 820, 984, 1304
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 3 cut(s) 244, 492, 1131
Alw44I GTGCAC 1 cut(s) 642
AlwI GGATC 2 cut(s) 884, 897
Ama87I CYCGRG 2 cut(s) 51, 558
AoxI GGCC 2 cut(s) 73, 778
ApaLI GTGCAC 1 cut(s) 642
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 3 cut(s) 265, 1008, 1163
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
AseI ATTAAT 1 cut(s) 1232
AspS9I GGNCC 1 cut(s) 1070
AsuHPI GGTGA 1 cut(s) 295
AsuII TTCGAA 1 cut(s) 92
AvaI CYCGRG 2 cut(s) 51, 558
AvaII GGWCC 1 cut(s) 1070
AxyI CCTNAGG 2 cut(s) 530, 1148
BaeGI GKGCMC 1 cut(s) 646
BbsI GAAGAC 1 cut(s) 840
Bbv12I GWGCWC 1 cut(s) 646
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 283
BccI CCATC 2 cut(s) 808, 1092
BcoDI GTCTC 3 cut(s) 244, 492, 1131
BfaI CTAG 3 cut(s) 369, 1001, 1059
BfmI CTRYAG 2 cut(s) 624, 1168
BfuAI ACCTGC 1 cut(s) 631
BglII AGATCT 1 cut(s) 1026
BisI GCNGC 2 cut(s) 297, 781
BlsI GCNGC 2 cut(s) 298, 782
Bme18I GGWCC 1 cut(s) 1070
BmeT110I CYCGRG 2 cut(s) 51, 558
BmgT120I GGNCC 1 cut(s) 1070
BmiI GGNNCC 2 cut(s) 104, 1071
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 2 cut(s) 65, 589
BmuI ACTGGG 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 840
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 631
BsaHI GRCGYC 1 cut(s) 237
BsaI GGTCTC 1 cut(s) 1131
BsaJI CCNNGG 1 cut(s) 775
BsaWI WCCGGW 1 cut(s) 468
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bse118I RCCGGY 2 cut(s) 75, 280
Bse1I ACTGG 3 cut(s) 656, 713, 891
Bse21I CCTNAGG 2 cut(s) 530, 1148
BseDI CCNNGG 1 cut(s) 775
BseGI GGATG 1 cut(s) 56
BseMII CTCAG 4 cut(s) 158, 290, 489, 1162
BseNI ACTGG 3 cut(s) 656, 713, 891
BseSI GKGCMC 1 cut(s) 646
BseXI GCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 100
BshFI GGCC 2 cut(s) 75, 780
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 2 cut(s) 51, 558
BsiSI CCGG 3 cut(s) 76, 281, 469
BslFI GGGAC 1 cut(s) 1195
BsmAI GTCTC 3 cut(s) 244, 492, 1131
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 1195
BsnI GGCC 2 cut(s) 75, 780
Bso31I GGTCTC 1 cut(s) 1131
BsoBI CYCGRG 2 cut(s) 51, 558
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 6 cut(s) 186, 440, 462, 876, 889, 1026
BspACI CCGC 2 cut(s) 639, 781
BspANI GGCC 2 cut(s) 75, 780
BspCNI CTCAG 4 cut(s) 157, 289, 488, 1161
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 2 cut(s) 104, 1071
BspMAI CTGCAG 2 cut(s) 628, 1172
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 2 cut(s) 884, 897
BspT104I TTCGAA 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 1131
BsrFI RCCGGY 2 cut(s) 75, 280
BsrI ACTGG 3 cut(s) 656, 713, 891
BssAI RCCGGY 2 cut(s) 75, 280
BssECI CCNNGG 1 cut(s) 775
BssMI GATC 6 cut(s) 186, 440, 462, 876, 889, 1026
BssNAI GTATAC 1 cut(s) 311
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 775
Bst1107I GTATAC 1 cut(s) 311
Bst4CI ACNGT 6 cut(s) 70, 415, 573, 729, 1156, 1285
Bst6I CTCTTC 1 cut(s) 696
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 3 cut(s) 83, 359, 637
BstDEI CTNAG 7 cut(s) 144, 276, 475, 530, 872, 1137, 1148
BstF5I GGATG 1 cut(s) 56
BstKTI GATC 6 cut(s) 189, 443, 465, 879, 892, 1029
BstMAI GTCTC 3 cut(s) 244, 492, 1131
BstMBI GATC 6 cut(s) 186, 440, 462, 876, 889, 1026
BstMWI GCNNNNNNNGC 4 cut(s) 189, 632, 641, 905
BstNSI RCATGY 2 cut(s) 639, 1274
BstSFI CTRYAG 2 cut(s) 624, 1168
BstSLI GKGCMC 1 cut(s) 646
BstV1I GCAGC 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 1 cut(s) 1026
BstYI RGATCY 1 cut(s) 1026
BstZ17I GTATAC 1 cut(s) 311
Bsu36I CCTNAGG 2 cut(s) 530, 1148
BsuRI GGCC 2 cut(s) 75, 780
BtsCI GGATG 1 cut(s) 56
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 2 cut(s) 517, 649
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 3 cut(s) 83, 359, 637
CciI TCATGA 1 cut(s) 936
Cfr10I RCCGGY 2 cut(s) 75, 280
Cfr13I GGNCC 1 cut(s) 1070
Csp6I GTAC 4 cut(s) 457, 502, 772, 1173
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviAII CATG 7 cut(s) 564, 636, 692, 789, 937, 1033, 1271
CviQI GTAC 4 cut(s) 457, 502, 772, 1173
DdeI CTNAG 7 cut(s) 144, 276, 475, 530, 872, 1137, 1148
DpnI GATC 6 cut(s) 188, 442, 464, 878, 891, 1028
DpnII GATC 6 cut(s) 186, 440, 462, 876, 889, 1026
DrdI GACNNNNNNGTC 2 cut(s) 671, 891
DseDI GACNNNNNNGTC 2 cut(s) 671, 891
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 775
Eco31I GGTCTC 1 cut(s) 1131
Eco47I GGWCC 1 cut(s) 1070
Eco81I CCTNAGG 2 cut(s) 530, 1148
Eco88I CYCGRG 2 cut(s) 51, 558
EcoT14I CCWWGG 1 cut(s) 775
ErhI CCWWGG 1 cut(s) 775
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 7 cut(s) 567, 639, 695, 792, 940, 1036, 1274
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 1 cut(s) 1195
FatI CATG 7 cut(s) 563, 635, 691, 788, 936, 1032, 1270
FblI GTMKAC 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 297, 781
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 297, 781
FspBI CTAG 3 cut(s) 369, 1001, 1059
GluI GCNGC 2 cut(s) 297, 781
HaeIII GGCC 2 cut(s) 75, 780
HapII CCGG 3 cut(s) 76, 281, 469
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 7 cut(s) 567, 639, 695, 792, 940, 1036, 1274
HinfI GANTC 8 cut(s) 43, 119, 548, 672, 678, 757, 809, 1046
HpaII CCGG 3 cut(s) 76, 281, 469
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 311, 644
Hpy188I TCNGA 9 cut(s) 28, 48, 147, 250, 478, 664, 677, 1045, 1051
Hpy188III TCNNGA 7 cut(s) 40, 610, 937, 952, 1001, 1014, 1150
Hpy8I GTNNAC 2 cut(s) 311, 644
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 33, 70, 1060
HpyCH4III ACNGT 6 cut(s) 70, 415, 573, 729, 1156, 1285
HpyCH4IV ACGT 3 cut(s) 237, 334, 1184
HpyF10VI GCNNNNNNNGC 4 cut(s) 189, 632, 641, 905
HpyF3I CTNAG 7 cut(s) 144, 276, 475, 530, 872, 1137, 1148
HpySE526I ACGT 3 cut(s) 237, 334, 1184
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 7 cut(s) 567, 639, 695, 792, 940, 1036, 1274
Kzo9I GATC 6 cut(s) 186, 440, 462, 876, 889, 1026
LmnI GCTCC 3 cut(s) 379, 817, 989
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 2 cut(s) 65, 589
MaeI CTAG 3 cut(s) 369, 1001, 1059
MaeII ACGT 3 cut(s) 237, 334, 1184
MaeIII GTNAC 3 cut(s) 227, 435, 1264
MalI GATC 6 cut(s) 188, 442, 464, 878, 891, 1028
MboI GATC 6 cut(s) 186, 440, 462, 876, 889, 1026
MboII GAAGA 4 cut(s) 298, 713, 845, 1021
MflI RGATCY 1 cut(s) 1026
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 6 cut(s) 196, 265, 420, 1008, 1163, 1229
MlyI GAGTC 2 cut(s) 681, 803
MmeI TCCRAC 2 cut(s) 267, 655
MseI TTAA 5 cut(s) 263, 428, 452, 1113, 1232
MslI CAYNNNNRTG 2 cut(s) 696, 727
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 3 cut(s) 76, 281, 469
MwoI GCNNNNNNNGC 4 cut(s) 189, 632, 641, 905
NdeII GATC 6 cut(s) 186, 440, 462, 876, 889, 1026
NlaIII CATG 7 cut(s) 567, 639, 695, 792, 940, 1036, 1274
NlaIV GGNNCC 2 cut(s) 104, 1071
NspI RCATGY 2 cut(s) 639, 1274
NspV TTCGAA 1 cut(s) 92
OliI CACNNNNGTG 1 cut(s) 727
PaeI GCATGC 1 cut(s) 639
PaeR7I CTCGAG 1 cut(s) 558
PagI TCATGA 1 cut(s) 936
PciI ACATGT 1 cut(s) 1270
PfeI GAWTC 6 cut(s) 43, 119, 548, 678, 757, 1046
PkrI GCNGC 2 cut(s) 298, 782
PleI GAGTC 2 cut(s) 680, 803
PpsI GAGTC 2 cut(s) 680, 803
PscI ACATGT 1 cut(s) 1270
PshBI ATTAAT 1 cut(s) 1232
PspN4I GGNNCC 2 cut(s) 104, 1071
PspPI GGNCC 1 cut(s) 1070
PspXI VCTCGAGB 1 cut(s) 558
PstI CTGCAG 2 cut(s) 628, 1172
PsuI RGATCY 1 cut(s) 1026
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 4 cut(s) 458, 503, 773, 1174
RsaNI GTAC 4 cut(s) 457, 502, 772, 1173
RseI CAYNNNNRTG 2 cut(s) 696, 727
SaqAI TTAA 5 cut(s) 263, 428, 452, 1113, 1232
SatI GCNGC 2 cut(s) 297, 781
Sau3AI GATC 6 cut(s) 186, 440, 462, 876, 889, 1026
Sau96I GGNCC 1 cut(s) 1070
SchI GAGTC 2 cut(s) 681, 803
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 2 cut(s) 65, 589
SfcI CTRYAG 2 cut(s) 624, 1168
Sfr274I CTCGAG 1 cut(s) 558
SfuI TTCGAA 1 cut(s) 92
SinI GGWCC 1 cut(s) 1070
SlaI CTCGAG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 696, 727
SmlI CTYRAG 2 cut(s) 558, 610
SmoI CTYRAG 2 cut(s) 558, 610
SphI GCATGC 1 cut(s) 639
Sse9I AATT 6 cut(s) 196, 265, 420, 1008, 1163, 1229
SsiI CCGC 2 cut(s) 639, 781
SspI AATATT 2 cut(s) 717, 960
SspMI CTAG 3 cut(s) 369, 1001, 1059
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 6 cut(s) 70, 415, 573, 729, 1156, 1285
TaiI ACGT 3 cut(s) 240, 337, 1187
TaqI TCGA 9 cut(s) 39, 63, 92, 185, 559, 741, 812, 1015, 1109
TasI AATT 6 cut(s) 196, 265, 420, 1008, 1163, 1229
TatI WGTACW 2 cut(s) 501, 1172
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 6 cut(s) 43, 119, 548, 678, 757, 1046
Tru1I TTAA 5 cut(s) 263, 428, 452, 1113, 1232
Tru9I TTAA 5 cut(s) 263, 428, 452, 1113, 1232
TscAI CASTG 2 cut(s) 524, 656
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 5 cut(s) 118, 163, 680, 714, 1021
TspRI CASTG 2 cut(s) 524, 656
VneI GTGCAC 1 cut(s) 642
VpaK11BI GGWCC 1 cut(s) 1070
VspI ATTAAT 1 cut(s) 1232
XapI RAATTY 3 cut(s) 265, 1008, 1163
XbaI TCTAGA 1 cut(s) 1000
XceI RCATGY 2 cut(s) 639, 1274
XcmI CCANNNNNNNNNTGG 1 cut(s) 1080
XhoI CTCGAG 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 310
XspI CTAG 3 cut(s) 369, 1001, 1059
ZraI GACGTC 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.