Rh2DG088800

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005421
Physical Location & Seq
Reverse (-)
6884379 .. 6885986
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG088800.1

Sequence Viewer

Length: 1008 bp
ATGGCTTCATCACAAACACTTCCTTCTGACCTCTATTTCGAGATTCTGACCCGAGCATCCTTCGATACTGTTGGCCGGTGCAGGCTCGTTTCGAAACAATGGAACCACCTTACATACGATTCAAGTTTCATAAATCAACTATCTCAGAGAACCAAAACAACACACGGGGTTTTCATTCAAAGCTCGATCAGCAGTAATTATATATCCACCTTTGTTCAAGCTGTGGGTAACAACGACGTCTCCAACTCCGAACAGCTGTCCTTAAATTTCCTGCCTCAGCCGGTGAAGATTGAAGCTGCTCATCAGGGTATACTCGTTTGTGTAAGCCAGAAACGTGTTCCTCAATACTATGTCTGCAAGCCAACCACTAGGGAATGGGAGCAGATACACAACCCAAAAACGAGGTATATGACGGTAAAAATTGCCTTAATGGTGTTACGATCAAGCCCGTTAAGGTACAAGATCGTCCGGTTCTCAGAACCAAAGTCTCCATATTCCAAGTACAAGTCGCAGGAACACTGCTACAACCTTAGGTGTGAGGTATATGATTCCAAAGCTCGAGCATGGAAACAGTTGAACAATGTAGCTTTACCCAAAGATGCTTATATCTTGAGGTTTCAACCTGCAGTATCGGCATGCGGTGCACTCCACTGGCTTTTGTCAGACAACCGAGTCGGAATCTTTGCTTTTCATGTAGATGAAGAGAACTGGGAAATATTTGCACCACCGTGCTTGGCAGTCGATAATGTCAATGGGAATCAGGTGGTGGAGTACCAAGGCCGCCTTGCATGGATTTGTGAAGTAGAGGACTCGATGGAGCTATGGGTTATGGAAGACTACAAGAACAAAGTATGGAGTAAAAGACAGATACTAAGGATCAAAGGACTGGATCGTCTTGCTTCTTTTGCAACTCTTTGCAATAATGATGTTGCACTCATGAGAGGATTTTTCAAGATAATATTTTACAAGTTTGGAGATTATAGCTCCAAGATAGCAAATCTAGAGTAA

Protein Analysis

335

Amino Acids

38.71

Weight (kDa)

9.01

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 6 - 47 3.7e-06 F-box-like
FBA_1 PF07734 94 - 287 2.1e-14 F-box associated beta propeller domain
FBA_3 PF08268 98 - 291 1.3e-14 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 99 - 240 1.1e-06 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 671, 891
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 310
AciI CCGC 2 cut(s) 639, 781
AclWI GGATC 2 cut(s) 884, 897
AcoI YGGCCR 1 cut(s) 73
AcsI RAATTY 1 cut(s) 265
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 3 cut(s) 458, 503, 773
AflIII ACRYGT 1 cut(s) 334
AgsI TTSAA 7 cut(s) 123, 179, 218, 293, 577, 620, 952
AleI CACNNNNGTG 1 cut(s) 727
AluBI AGCT 8 cut(s) 183, 221, 256, 296, 557, 587, 820, 984
AluI AGCT 8 cut(s) 183, 221, 256, 296, 557, 587, 820, 984
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 2 cut(s) 244, 492
Alw44I GTGCAC 1 cut(s) 642
AlwI GGATC 2 cut(s) 884, 897
Ama87I CYCGRG 2 cut(s) 51, 558
AoxI GGCC 2 cut(s) 73, 778
ApaLI GTGCAC 1 cut(s) 642
ApeKI GCWGC 1 cut(s) 296
ApoI RAATTY 1 cut(s) 265
ArsI GACNNNNNNTTYG 2 cut(s) 20, 52
AsuHPI GGTGA 1 cut(s) 295
AsuII TTCGAA 1 cut(s) 92
AvaI CYCGRG 2 cut(s) 51, 558
AxyI CCTNAGG 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 646
BbsI GAAGAC 1 cut(s) 840
Bbv12I GWGCWC 1 cut(s) 646
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 283
BccI CCATC 1 cut(s) 808
BcoDI GTCTC 2 cut(s) 244, 492
BfaI CTAG 2 cut(s) 369, 1001
BfmI CTRYAG 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 631
BisI GCNGC 2 cut(s) 297, 781
BlsI GCNGC 2 cut(s) 298, 782
BmeT110I CYCGRG 2 cut(s) 51, 558
BmiI GGNNCC 1 cut(s) 104
BmrI ACTGGG 1 cut(s) 718
BmsI GCATC 2 cut(s) 65, 589
BmuI ACTGGG 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 840
Bpu10I CCTNAGC 1 cut(s) 276
Bpu14I TTCGAA 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 631
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 775
BsaWI WCCGGW 1 cut(s) 468
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bse118I RCCGGY 2 cut(s) 75, 280
Bse1I ACTGG 3 cut(s) 656, 713, 891
Bse21I CCTNAGG 1 cut(s) 530
BseDI CCNNGG 1 cut(s) 775
BseGI GGATG 1 cut(s) 56
BseMII CTCAG 3 cut(s) 158, 290, 489
BseNI ACTGG 3 cut(s) 656, 713, 891
BseSI GKGCMC 1 cut(s) 646
BseXI GCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 100
BshFI GGCC 2 cut(s) 75, 780
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 2 cut(s) 51, 558
BsiSI CCGG 3 cut(s) 76, 281, 469
BsmAI GTCTC 2 cut(s) 244, 492
BsmBI CGTCTC 1 cut(s) 244
BsnI GGCC 2 cut(s) 75, 780
BsoBI CYCGRG 2 cut(s) 51, 558
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 5 cut(s) 186, 440, 462, 876, 889
BspACI CCGC 2 cut(s) 639, 781
BspANI GGCC 2 cut(s) 75, 780
BspCNI CTCAG 3 cut(s) 157, 289, 488
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 1 cut(s) 104
BspMAI CTGCAG 1 cut(s) 628
BspMI ACCTGC 1 cut(s) 631
BspPI GGATC 2 cut(s) 884, 897
BspT104I TTCGAA 1 cut(s) 92
BsrFI RCCGGY 2 cut(s) 75, 280
BsrI ACTGG 3 cut(s) 656, 713, 891
BssAI RCCGGY 2 cut(s) 75, 280
BssECI CCNNGG 1 cut(s) 775
BssMI GATC 5 cut(s) 186, 440, 462, 876, 889
BssNAI GTATAC 1 cut(s) 311
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 775
Bst1107I GTATAC 1 cut(s) 311
Bst4CI ACNGT 4 cut(s) 70, 415, 573, 729
Bst6I CTCTTC 1 cut(s) 696
BstACI GRCGYC 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 3 cut(s) 83, 359, 637
BstDEI CTNAG 5 cut(s) 144, 276, 475, 530, 872
BstF5I GGATG 1 cut(s) 56
BstKTI GATC 5 cut(s) 189, 443, 465, 879, 892
BstMAI GTCTC 2 cut(s) 244, 492
BstMBI GATC 5 cut(s) 186, 440, 462, 876, 889
BstMWI GCNNNNNNNGC 4 cut(s) 189, 632, 641, 905
BstNSI RCATGY 1 cut(s) 639
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 1 cut(s) 646
BstV1I GCAGC 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 840
BstZ17I GTATAC 1 cut(s) 311
Bsu36I CCTNAGG 1 cut(s) 530
BsuRI GGCC 2 cut(s) 75, 780
BtsCI GGATG 1 cut(s) 56
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 2 cut(s) 517, 649
BveI ACCTGC 1 cut(s) 631
Cac8I GCNNGC 3 cut(s) 83, 359, 637
CciI TCATGA 1 cut(s) 936
Cfr10I RCCGGY 2 cut(s) 75, 280
Csp6I GTAC 3 cut(s) 457, 502, 772
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviAII CATG 5 cut(s) 564, 636, 692, 789, 937
CviQI GTAC 3 cut(s) 457, 502, 772
DdeI CTNAG 5 cut(s) 144, 276, 475, 530, 872
DpnI GATC 5 cut(s) 188, 442, 464, 878, 891
DpnII GATC 5 cut(s) 186, 440, 462, 876, 889
DrdI GACNNNNNNGTC 2 cut(s) 671, 891
DseDI GACNNNNNNGTC 2 cut(s) 671, 891
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 775
Eco81I CCTNAGG 1 cut(s) 530
Eco88I CYCGRG 2 cut(s) 51, 558
EcoT14I CCWWGG 1 cut(s) 775
ErhI CCWWGG 1 cut(s) 775
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 5 cut(s) 567, 639, 695, 792, 940
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FatI CATG 5 cut(s) 563, 635, 691, 788, 936
FblI GTMKAC 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 297, 781
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 2 cut(s) 297, 781
FspBI CTAG 2 cut(s) 369, 1001
GluI GCNGC 2 cut(s) 297, 781
HaeIII GGCC 2 cut(s) 75, 780
HapII CCGG 3 cut(s) 76, 281, 469
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 5 cut(s) 567, 639, 695, 792, 940
HinfI GANTC 7 cut(s) 43, 119, 548, 672, 678, 757, 809
HpaII CCGG 3 cut(s) 76, 281, 469
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 2 cut(s) 311, 644
Hpy188I TCNGA 7 cut(s) 28, 48, 147, 250, 478, 664, 677
Hpy188III TCNNGA 5 cut(s) 40, 610, 937, 952, 1001
Hpy8I GTNNAC 2 cut(s) 311, 644
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 2 cut(s) 33, 70
HpyCH4III ACNGT 4 cut(s) 70, 415, 573, 729
HpyCH4IV ACGT 2 cut(s) 237, 334
HpyCH4V TGCA 9 cut(s) 81, 357, 626, 644, 722, 788, 908, 918, 932
HpyF10VI GCNNNNNNNGC 4 cut(s) 189, 632, 641, 905
HpyF3I CTNAG 5 cut(s) 144, 276, 475, 530, 872
HpySE526I ACGT 2 cut(s) 237, 334
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 5 cut(s) 567, 639, 695, 792, 940
Kzo9I GATC 5 cut(s) 186, 440, 462, 876, 889
LmnI GCTCC 3 cut(s) 379, 817, 989
Lsp1109I GCAGC 1 cut(s) 283
LweI GCATC 2 cut(s) 65, 589
MaeI CTAG 2 cut(s) 369, 1001
MaeII ACGT 2 cut(s) 237, 334
MaeIII GTNAC 2 cut(s) 227, 435
MalI GATC 5 cut(s) 188, 442, 464, 878, 891
MboI GATC 5 cut(s) 186, 440, 462, 876, 889
MboII GAAGA 3 cut(s) 298, 713, 845
MhlI GDGCHC 1 cut(s) 646
MluCI AATT 3 cut(s) 196, 265, 420
MlyI GAGTC 2 cut(s) 681, 803
MmeI TCCRAC 2 cut(s) 267, 655
MnlI CCTC 8 cut(s) 41, 285, 351, 396, 532, 606, 799, 935
MseI TTAA 3 cut(s) 263, 428, 452
MslI CAYNNNNRTG 2 cut(s) 696, 727
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 3 cut(s) 76, 281, 469
MwoI GCNNNNNNNGC 4 cut(s) 189, 632, 641, 905
NdeII GATC 5 cut(s) 186, 440, 462, 876, 889
NlaIII CATG 5 cut(s) 567, 639, 695, 792, 940
NlaIV GGNNCC 1 cut(s) 104
NspI RCATGY 1 cut(s) 639
NspV TTCGAA 1 cut(s) 92
OliI CACNNNNGTG 1 cut(s) 727
PaeI GCATGC 1 cut(s) 639
PaeR7I CTCGAG 1 cut(s) 558
PagI TCATGA 1 cut(s) 936
PfeI GAWTC 5 cut(s) 43, 119, 548, 678, 757
PkrI GCNGC 2 cut(s) 298, 782
PleI GAGTC 2 cut(s) 680, 803
PpsI GAGTC 2 cut(s) 680, 803
PspN4I GGNNCC 1 cut(s) 104
PspXI VCTCGAGB 1 cut(s) 558
PstI CTGCAG 1 cut(s) 628
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 3 cut(s) 458, 503, 773
RsaNI GTAC 3 cut(s) 457, 502, 772
RseI CAYNNNNRTG 2 cut(s) 696, 727
SaqAI TTAA 3 cut(s) 263, 428, 452
SatI GCNGC 2 cut(s) 297, 781
Sau3AI GATC 5 cut(s) 186, 440, 462, 876, 889
SchI GAGTC 2 cut(s) 681, 803
SduI GDGCHC 1 cut(s) 646
SfaNI GCATC 2 cut(s) 65, 589
SfcI CTRYAG 1 cut(s) 624
Sfr274I CTCGAG 1 cut(s) 558
SfuI TTCGAA 1 cut(s) 92
SlaI CTCGAG 1 cut(s) 558
SmiMI CAYNNNNRTG 2 cut(s) 696, 727
SmlI CTYRAG 2 cut(s) 558, 610
SmoI CTYRAG 2 cut(s) 558, 610
SphI GCATGC 1 cut(s) 639
Sse9I AATT 3 cut(s) 196, 265, 420
SsiI CCGC 2 cut(s) 639, 781
SspI AATATT 2 cut(s) 717, 960
SspMI CTAG 2 cut(s) 369, 1001
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 4 cut(s) 70, 415, 573, 729
TaiI ACGT 2 cut(s) 240, 337
TaqI TCGA 7 cut(s) 39, 63, 92, 185, 559, 741, 812
TasI AATT 3 cut(s) 196, 265, 420
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 5 cut(s) 43, 119, 548, 678, 757
Tru1I TTAA 3 cut(s) 263, 428, 452
Tru9I TTAA 3 cut(s) 263, 428, 452
TscAI CASTG 2 cut(s) 524, 656
TseI GCWGC 1 cut(s) 296
TspDTI ATGAA 4 cut(s) 118, 163, 680, 714
TspRI CASTG 2 cut(s) 524, 656
VneI GTGCAC 1 cut(s) 642
XapI RAATTY 1 cut(s) 265
XbaI TCTAGA 1 cut(s) 1000
XceI RCATGY 1 cut(s) 639
XhoI CTCGAG 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 310
XspI CTAG 2 cut(s) 369, 1001
ZraI GACGTC 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.