Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003263

Physical Location & Seq

Genomic Coordinates Forward (+)

8789828 .. 8792721

Gene Structure 2894 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1109100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGTGGGGAGCTGTTTTTGTAGCCATTGCTGCTGCGACAGGAAACTTGTTGCAAGGATGGGACAATGCAACCATTGCAGGTACTATTTCTATGATGGGGCTGCTTTACATCAAAAGGGAATTTAAGTTGGAAACCCAACCGACAATTGAAGGGCTTATCGTAGGCATGTCTTTGATCGGTGCCACTGTCATCACAACATTCTCAGGGCCAGTTTCAGACTGGCTAGGAAGACGTCCAATGCTGATAGTTTCATCAGTTTTATATTTTCTCAATGGTTTGGTAATGCTATGGGCTCCAAATGTCTATGTTCTTCTTTTGGCAAGGCTACTAGATGGTTTTGGAATTGGACTAGCCGTTACTCTTGTCCCGTTTTACATTTCCGAAACTGCCCCACCCGAAATCAGAGGGCTACTGAACACTCTCCCACAGTTTACCGGTTCGGGAGGGATGTTTTTGTCATACTGCATGATGTTTGGAATGTCACTGAAGGATGCCCCCAGTTGGAGAATGATGCTTGGATCTCCACGGTGGCTTGTTAGTAAAGGAAGAATGGCTGAGGCTAAGAAAGTTCAGCAGAGACTACCTGGACAGGAAGATGTTTCAGGTGAGATGGCTTTGCCGTTCGAGGGACTTGGTGTTGGAGGGGAAGCATCATTGGAAGAGTACATAATTGGACCGGCTAATGAGGGCAGTGAAGGCCAGGAAATGACTTCGGATAAAGATCAAATAAGGCTGTATGGACAGGAGGATGGGAGGGCTTCAATGCTCGAGGGGAGGGCGTCAATGATTGCGAAATCTGTCACAGGACAAGCCAATCTTGGCTTGATGTCGCGACAAGGAAGCTTGTTGAATCCAAACGTGCATTACGTGGATCCTCTTGTGAATCTATTTGGCAGTGTTCATGAGAAGCTTCTTCCCGAAAACACAGGAAGTATGTGCAGCCTTCTTAATTTGCAGAACATGGGCAGCATTCTCAACATGGGCAGAGTGCTAAATGTGGCGCTGCGTGGACGCCAAGACAAGATGGAGAATTGGGATTTGGAGGGGAATGGAAATGCAGCTGATTTTGAGGACAATGTTCGAAGGCCATTGCTCTCAGGTCAAAAATCAGCAAGTATGGTAAATGACATGATGCCTAGTAAGTCCAATAGCTTATTTGGCATGAGGCACAATAGCAGCCTCATGCTGGGGACTAGTGGTGAAGCTGTGAGTAGTATGGACATTGGCGGTGGCTGGGCGTTGGCTTACAAATACTCTGAGAAGGTTGGAAAAGATGGAAAAAAGACAGAAGAGTACCAAAGATTCTCTGGCATAAATGGGGTTCTTTATTACACTCCCCAAATTCTTGAGCAAGCAGGGGTGGCAATTCTCCTATCGGATTTAGGCCTTAGTTCCACCTCTTCATCTATTCTCATAAGTGCTCTCACAACCCTATTGATGATTCCGTCTATCGGCATTGCCACGAGACTAATGGATACTTCTGGCAGAAGGTCGCTGTTGTTGACTACATTACCGGTGCTAACTGGCACCCTCCTAGTACTAATTTTCGGACAACTTGTCCATTTGGGTTCCGTTGTCAATGCAACAATTTCTACAATCAGCATTGTGGTCTACTTCTGCACCTTTGTCATGGGGTTCGGACCAATCCCTAACATCCTTTGCTCGAAGATCTTCCCGACTCAGGTTCGTGGCCTCTGCATTGCAATATGTGCCCTGACATTTTGGATTGGAGACATCATTATGACTTACACACTGCCTATCATGCTCACCTCGATTGGCCTCGCCAGTGTTTTTGCTATTTATGCTGTTGTGTGCACCATTTCTTGGGTGTTTGTTTTCTTCAAAGTGCCAGAAACAAAGGGAATGCCGTTAGAAGTTATCTCTGAGTTTTTTCTGTGGGTGCAAAGCAGGCAGAAATTGATGAAAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

644

Amino Acids

69.72

Weight (kDa)

6.2

Isoelectric Point (pI)

37.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	8 - 205	8.5e-36	Sugar (and other) transporter
MFS_1	PF07690	28 - 170	4.5e-12	Major Facilitator Superfamily
Sugar_tr	PF00083	433 - 631	6.3e-32	Sugar (and other) transporter
MFS_1	PF07690	456 - 620	2.9e-10	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003263)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g10110
malus_domestica	MD02G1109100.v1.1 MD02G1109500.v1.1 MD15G1228300.v1.1
prunus_persica	Prupe.7G186000_v2.0.a1
pyrus_communis	pycom15g20340
rosa_chinensis	RchiOBHm_Chr1g0348791 RchiOBHm_Chr2g0096881 RchiOBHm_Chr2g0147991 RchiOBHm_Chr4g0410581 RchiOBHm_Chr7g0216381
rosa_laevigata	RLG00000016678
rosa_multiflora	Rmu_sc0000692.1_g000002 Rmu_sc0003743.1_g000031 Rmu_sc0007264.1_g000009 Rmu_sc0007806.1_g000019 Rmu_ssc0000215.1_g000006 Rmu_ssc0000433.1_g000003
rosa_roxburghii	Rroxscaffold_2G00145190
rosa_rugosa	Rorug02G0061700
rosa_samantha	Rh1AG316900 Rh2AG109400 Rh2AG598500 Rh2BG111400 Rh2CG113500 Rh2DG112500 Rh4CG174300 Rh5BG370000 Rh5DG336900 Rh5DG401500 Rh5DG401600 Rh6DG244100 Rh7DG300200
rosa_wichuraiana	Rw1G028090 Rw2G008450 Rw7G025410 Rw7G025420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	235
Acc36I	ACCTGC	1 cut(s)	68
AccB1I	GGYRCC	2 cut(s)	179, 1526
AccB7I	CCANNNNNTGG	1 cut(s)	1824
AccI	GTMKAC	1 cut(s)	1611
AccII	CGCG	1 cut(s)	832
AciI	CCGC	1 cut(s)	1227
AclWI	GGATC	3 cut(s)	526, 866, 879
AcsI	RAATTY	2 cut(s)	119, 1341
AcuI	CTGAAG	1 cut(s)	506
AcyI	GRCGYC	3 cut(s)	232, 779, 1012
AfaI	GTAC	4 cut(s)	82, 665, 1295, 1539
AfiI	CCNNNNNNNGG	6 cut(s)	501, 626, 1186, 1451, 1681, 1824
AgeI	ACCGGT	2 cut(s)	434, 1513
AgsI	TTSAA	4 cut(s)	149, 762, 850, 1843
AhdI	GACNNNNNGTC	1 cut(s)	1556
AhlI	ACTAGT	1 cut(s)	1193
AjnI	CCWGG	2 cut(s)	583, 699
AjuI	GAANNNNNNNTTGG	2 cut(s)	1022, 1054
AluBI	AGCT	6 cut(s)	11, 843, 910, 1061, 1152, 1205
AluI	AGCT	6 cut(s)	11, 843, 910, 1061, 1152, 1205
Alw21I	GWGCWC	2 cut(s)	1423, 1817
Alw26I	GTCTC	3 cut(s)	571, 1459, 1725
Alw44I	GTGCAC	1 cut(s)	1813
AlwI	GGATC	3 cut(s)	526, 866, 879
Ama87I	CYCGRG	1 cut(s)	767
AoxI	GGCC	6 cut(s)	206, 697, 1085, 1384, 1690, 1777
ApaLI	GTGCAC	1 cut(s)	1813
ApeKI	GCWGC	8 cut(s)	29, 32, 100, 939, 966, 1003, 1058, 1176
ApoI	RAATTY	2 cut(s)	119, 1341
AsiGI	ACCGGT	2 cut(s)	434, 1513
Asp700I	GAANNNNTTC	1 cut(s)	1670
AspLEI	GCGC	1 cut(s)	1003
AspS9I	GGNCC	3 cut(s)	206, 674, 1640
AsuHPI	GGTGA	3 cut(s)	617, 1211, 1759
AsuII	TTCGAA	1 cut(s)	1081
AvaI	CYCGRG	1 cut(s)	767
AvaII	GGWCC	2 cut(s)	674, 1640
BaeGI	GKGCMC	2 cut(s)	1714, 1817
BamHI	GGATCC	1 cut(s)	871
BanI	GGYRCC	2 cut(s)	179, 1526
BanII	GRGCYC	1 cut(s)	295
BauI	CACGAG	1 cut(s)	1462
BbsI	GAAGAC	1 cut(s)	235
Bbv12I	GWGCWC	2 cut(s)	1423, 1817
BbvCI	CCTCAGC	1 cut(s)	555
BbvI	GCAGC	8 cut(s)	16, 19, 87, 951, 978, 990, 1070, 1188
BccI	CCATC	7 cut(s)	51, 88, 326, 604, 743, 1018, 1268
BceAI	ACGGC	3 cut(s)	338, 604, 1852
BciT130I	CCWGG	2 cut(s)	585, 701
BciVI	GTATCC	1 cut(s)	1468
BcoDI	GTCTC	3 cut(s)	571, 1459, 1725
BcuI	ACTAGT	1 cut(s)	1193
BfaI	CTAG	6 cut(s)	224, 329, 350, 1137, 1194, 1535
BfoI	RGCGCY	1 cut(s)	1004
BfuAI	ACCTGC	1 cut(s)	68
BfuI	GTATCC	1 cut(s)	1468
BglII	AGATCT	1 cut(s)	1668
BisI	GCNGC	8 cut(s)	30, 33, 101, 940, 967, 1004, 1059, 1177
BlsI	GCNGC	8 cut(s)	31, 34, 102, 941, 968, 1005, 1060, 1178
BmcAI	AGTACT	1 cut(s)	1539
Bme1390I	CCNGG	2 cut(s)	585, 701
Bme18I	GGWCC	2 cut(s)	674, 1640
BmeRI	GACNNNNNGTC	1 cut(s)	1556
BmeT110I	CYCGRG	1 cut(s)	767
BmgT120I	GGNCC	3 cut(s)	206, 674, 1640
BmiI	GGNNCC	5 cut(s)	181, 294, 873, 1528, 1570
BmrFI	CCNGG	2 cut(s)	585, 701
BmrI	ACTGGG	1 cut(s)	492
BmsI	GCATC	4 cut(s)	481, 501, 659, 1122
BmuI	ACTGGG	1 cut(s)	492
BpiI	GAAGAC	1 cut(s)	235
Bpu10I	CCTNAGC	1 cut(s)	555
Bpu14I	TTCGAA	1 cut(s)	1081
BpuEI	CTTGAG	1 cut(s)	1367
BsaAI	YACGTR	1 cut(s)	868
BsaBI	GATNNNNATC	1 cut(s)	720
BsaHI	GRCGYC	3 cut(s)	232, 779, 1012
BsaJI	CCNNGG	1 cut(s)	524
BsaWI	WCCGGW	2 cut(s)	434, 1513
Bsc4I	CCNNNNNNNGG	6 cut(s)	501, 626, 1186, 1451, 1681, 1824
Bse118I	RCCGGY	3 cut(s)	434, 676, 1513
Bse1I	ACTGG	5 cut(s)	209, 224, 498, 1528, 1785
Bse3DI	GCAATG	5 cut(s)	24, 72, 1088, 1455, 1698
Bse8I	GATNNNNATC	1 cut(s)	720
BseBI	CCWGG	2 cut(s)	585, 701
BseDI	CCNNGG	1 cut(s)	524
BseGI	GGATG	5 cut(s)	62, 453, 496, 754, 1653
BseJI	GATNNNNATC	1 cut(s)	720
BseLI	CCNNNNNNNGG	6 cut(s)	501, 626, 1186, 1451, 1681, 1824
BseMI	GCAATG	5 cut(s)	24, 72, 1088, 1455, 1698
BseMII	CTCAG	6 cut(s)	216, 546, 1110, 1248, 1694, 1875
BseNI	ACTGG	5 cut(s)	209, 224, 498, 1528, 1785
BseSI	GKGCMC	2 cut(s)	1714, 1817
BseXI	GCAGC	8 cut(s)	16, 19, 87, 951, 978, 990, 1070, 1188
BseYI	CCCAGC	2 cut(s)	1186, 1233
BsgI	GTGCAG	2 cut(s)	958, 1603
Bsh1236I	CGCG	1 cut(s)	832
BshFI	GGCC	6 cut(s)	208, 699, 1087, 1386, 1692, 1779
BshNI	GGYRCC	2 cut(s)	179, 1526
BshTI	ACCGGT	2 cut(s)	434, 1513
BsiHKAI	GWGCWC	2 cut(s)	1423, 1817
BsiHKCI	CYCGRG	1 cut(s)	767
BsiSI	CCGG	3 cut(s)	435, 677, 1514
BslFI	GGGAC	4 cut(s)	74, 350, 642, 1204
BslI	CCNNNNNNNGG	6 cut(s)	501, 626, 1186, 1451, 1681, 1824
BsmAI	GTCTC	3 cut(s)	571, 1459, 1725
BsmFI	GGGAC	4 cut(s)	74, 350, 642, 1204
BsmI	GAATGC	2 cut(s)	969, 1869
BsnI	GGCC	6 cut(s)	208, 699, 1087, 1386, 1692, 1779
BsoBI	CYCGRG	1 cut(s)	767
Bsp119I	TTCGAA	1 cut(s)	1081
Bsp1286I	GDGCHC	4 cut(s)	295, 1423, 1714, 1817
Bsp143I	GATC	5 cut(s)	174, 518, 721, 871, 1668
Bsp68I	TCGCGA	1 cut(s)	832
BspACI	CCGC	1 cut(s)	1227
BspANI	GGCC	6 cut(s)	208, 699, 1087, 1386, 1692, 1779
BspCNI	CTCAG	6 cut(s)	215, 547, 1109, 1249, 1693, 1876
BspFNI	CGCG	1 cut(s)	832
BspHI	TCATGA	1 cut(s)	901
BspLI	GGNNCC	5 cut(s)	181, 294, 873, 1528, 1570
BspMI	ACCTGC	1 cut(s)	68
BspPI	GGATC	3 cut(s)	526, 866, 879
BspT104I	TTCGAA	1 cut(s)	1081
BspT107I	GGYRCC	2 cut(s)	179, 1526
BsrDI	GCAATG	5 cut(s)	24, 72, 1088, 1455, 1698
BsrFI	RCCGGY	3 cut(s)	434, 676, 1513
BsrI	ACTGG	5 cut(s)	209, 224, 498, 1528, 1785
BssAI	RCCGGY	3 cut(s)	434, 676, 1513
BssECI	CCNNGG	1 cut(s)	524
BssMI	GATC	5 cut(s)	174, 518, 721, 871, 1668
BssNI	GRCGYC	3 cut(s)	232, 779, 1012
BssSI	CACGAG	1 cut(s)	1462
Bst2BI	CACGAG	1 cut(s)	1462
Bst2UI	CCWGG	2 cut(s)	585, 701
Bst4CI	ACNGT	3 cut(s)	187, 429, 528
Bst6I	CTCTTC	3 cut(s)	654, 1284, 1405
BstACI	GRCGYC	3 cut(s)	232, 779, 1012
BstAPI	GCANNNNNTGC	2 cut(s)	74, 1709
BstBAI	YACGTR	1 cut(s)	868
BstBI	TTCGAA	1 cut(s)	1081
BstC8I	GCNNGC	2 cut(s)	1353, 1910
BstDEI	CTNAG	8 cut(s)	202, 555, 561, 1096, 1257, 1388, 1680, 1884
BstDSI	CCRYGG	1 cut(s)	524
BstF5I	GGATG	5 cut(s)	62, 453, 496, 754, 1653
BstFNI	CGCG	1 cut(s)	832
BstH2I	RGCGCY	1 cut(s)	1004
BstHHI	GCGC	1 cut(s)	1003
BstKTI	GATC	5 cut(s)	177, 521, 724, 874, 1671
BstMAI	GTCTC	3 cut(s)	571, 1459, 1725
BstMBI	GATC	5 cut(s)	174, 518, 721, 871, 1668
BstMWI	GCNNNNNNNGC	9 cut(s)	29, 74, 696, 1158, 1361, 1709, 1762, 1802, 1909
BstNI	CCWGG	2 cut(s)	585, 701
BstNSI	RCATGY	1 cut(s)	169
BstSCI	CCNGG	2 cut(s)	583, 699
BstSLI	GKGCMC	2 cut(s)	1714, 1817
BstUI	CGCG	1 cut(s)	832
BstV1I	GCAGC	8 cut(s)	16, 19, 87, 951, 978, 990, 1070, 1188
BstV2I	GAAGAC	1 cut(s)	235
BstX2I	RGATCY	3 cut(s)	518, 871, 1668
BstYI	RGATCY	3 cut(s)	518, 871, 1668
BsuI	GTATCC	1 cut(s)	1468
BsuRI	GGCC	6 cut(s)	208, 699, 1087, 1386, 1692, 1779
BtgI	CCRYGG	1 cut(s)	524
BtsCI	GGATG	5 cut(s)	62, 453, 496, 754, 1653
BtsI	GCAGTG	3 cut(s)	697, 901, 1751
BtsIMutI	CAGTG	6 cut(s)	183, 482, 697, 901, 1751, 1792
BtuMI	TCGCGA	1 cut(s)	832
BveI	ACCTGC	1 cut(s)	68
Cac8I	GCNNGC	2 cut(s)	1353, 1910
CciI	TCATGA	1 cut(s)	901
CfoI	GCGC	1 cut(s)	1003
Cfr10I	RCCGGY	3 cut(s)	434, 676, 1513
Cfr13I	GGNCC	3 cut(s)	206, 674, 1640
CseI	GACGC	2 cut(s)	768, 1020
Csp6I	GTAC	4 cut(s)	81, 664, 1294, 1538
CspAI	ACCGGT	2 cut(s)	434, 1513
CviQI	GTAC	4 cut(s)	81, 664, 1294, 1538
DdeI	CTNAG	8 cut(s)	202, 555, 561, 1096, 1257, 1388, 1680, 1884
DpnI	GATC	5 cut(s)	176, 520, 723, 873, 1670
DpnII	GATC	5 cut(s)	174, 518, 721, 871, 1668
DriI	GACNNNNNGTC	1 cut(s)	1556
Eam1104I	CTCTTC	3 cut(s)	654, 1284, 1405
Eam1105I	GACNNNNNGTC	1 cut(s)	1556
EarI	CTCTTC	3 cut(s)	654, 1284, 1405
Eco147I	AGGCCT	1 cut(s)	1386
Eco24I	GRGCYC	1 cut(s)	295
Eco47I	GGWCC	2 cut(s)	674, 1640
Eco57I	CTGAAG	1 cut(s)	506
Eco88I	CYCGRG	1 cut(s)	767
EcoRII	CCWGG	2 cut(s)	583, 699
EcoT38I	GRGCYC	1 cut(s)	295
FalI	AAGNNNNNCTT	2 cut(s)	801, 833
FaqI	GGGAC	4 cut(s)	74, 350, 642, 1204
FblI	GTMKAC	1 cut(s)	1611
Fnu4HI	GCNGC	8 cut(s)	30, 33, 101, 940, 967, 1004, 1059, 1177
FokI	GGATG	5 cut(s)	69, 460, 503, 761, 1640
FriOI	GRGCYC	1 cut(s)	295
Fsp4HI	GCNGC	8 cut(s)	30, 33, 101, 940, 967, 1004, 1059, 1177
FspBI	CTAG	6 cut(s)	224, 329, 350, 1137, 1194, 1535
GlaI	GCGC	1 cut(s)	1002
GluI	GCNGC	8 cut(s)	30, 33, 101, 940, 967, 1004, 1059, 1177
GsaI	CCCAGC	2 cut(s)	1190, 1237
HaeII	RGCGCY	1 cut(s)	1004
HaeIII	GGCC	6 cut(s)	208, 699, 1087, 1386, 1692, 1779
HapII	CCGG	3 cut(s)	435, 677, 1514
HgaI	GACGC	2 cut(s)	768, 1020
HhaI	GCGC	1 cut(s)	1003
Hin1I	GRCGYC	3 cut(s)	232, 779, 1012
Hin6I	GCGC	1 cut(s)	1001
HinP1I	GCGC	1 cut(s)	1001
HincII	GTYRAC	1 cut(s)	1503
HindII	GTYRAC	1 cut(s)	1503
HindIII	AAGCTT	2 cut(s)	841, 908
HinfI	GANTC	5 cut(s)	850, 883, 1302, 1441, 1678
HpaII	CCGG	3 cut(s)	435, 677, 1514
HphI	GGTGA	3 cut(s)	617, 1211, 1759
Hpy166II	GTNNAC	5 cut(s)	432, 1010, 1503, 1612, 1815
Hpy188I	TCNGA	9 cut(s)	217, 382, 404, 715, 1258, 1378, 1550, 1640, 1885
Hpy188III	TCNNGA	6 cut(s)	441, 831, 902, 917, 1346, 1675
Hpy8I	GTNNAC	5 cut(s)	432, 1010, 1503, 1612, 1815
HpyAV	CCTTC	7 cut(s)	143, 481, 689, 953, 1077, 1255, 1482
HpyCH4III	ACNGT	3 cut(s)	187, 429, 528
HpyCH4IV	ACGT	3 cut(s)	232, 858, 867
HpyF10VI	GCNNNNNNNGC	9 cut(s)	29, 74, 696, 1158, 1361, 1709, 1762, 1802, 1909
HpyF3I	CTNAG	8 cut(s)	202, 555, 561, 1096, 1257, 1388, 1680, 1884
HpySE526I	ACGT	3 cut(s)	232, 858, 867
Hsp92I	GRCGYC	3 cut(s)	232, 779, 1012
HspAI	GCGC	1 cut(s)	1001
Kzo9I	GATC	5 cut(s)	174, 518, 721, 871, 1668
LmnI	GCTCC	2 cut(s)	8, 298
Lsp1109I	GCAGC	8 cut(s)	16, 19, 87, 951, 978, 990, 1070, 1188
LweI	GCATC	4 cut(s)	481, 501, 659, 1122
MaeI	CTAG	6 cut(s)	224, 329, 350, 1137, 1194, 1535
MaeII	ACGT	3 cut(s)	232, 858, 867
MaeIII	GTNAC	3 cut(s)	355, 480, 799
MalI	GATC	5 cut(s)	176, 520, 723, 873, 1670
MboI	GATC	5 cut(s)	174, 518, 721, 871, 1668
MfeI	CAATTG	1 cut(s)	144
MflI	RGATCY	3 cut(s)	518, 871, 1668
MhlI	GDGCHC	4 cut(s)	295, 1423, 1714, 1817
MlyI	GAGTC	1 cut(s)	1672
MmeI	TCCRAC	4 cut(s)	108, 482, 619, 1246
MroXI	GAANNNNTTC	1 cut(s)	1670
MseI	TTAA	2 cut(s)	123, 948
MslI	CAYNNNNRTG	1 cut(s)	1739
MspA1I	CMGCKG	1 cut(s)	1061
MspI	CCGG	3 cut(s)	435, 677, 1514
MspR9I	CCNGG	2 cut(s)	585, 701
MunI	CAATTG	1 cut(s)	144
Mva1269I	GAATGC	2 cut(s)	969, 1869
MvaI	CCWGG	2 cut(s)	585, 701
MvnI	CGCG	1 cut(s)	832
MwoI	GCNNNNNNNGC	9 cut(s)	29, 74, 696, 1158, 1361, 1709, 1762, 1802, 1909
NdeII	GATC	5 cut(s)	174, 518, 721, 871, 1668
NlaIV	GGNNCC	5 cut(s)	181, 294, 873, 1528, 1570
NmuCI	GTSAC	2 cut(s)	480, 799
NruI	TCGCGA	1 cut(s)	832
NspI	RCATGY	1 cut(s)	169
NspV	TTCGAA	1 cut(s)	1081
PaeR7I	CTCGAG	1 cut(s)	767
PagI	TCATGA	1 cut(s)	901
PceI	AGGCCT	1 cut(s)	1386
PcsI	WCGNNNNNNNCGW	1 cut(s)	864
PctI	GAATGC	2 cut(s)	969, 1869
PdmI	GAANNNNTTC	1 cut(s)	1670
PfeI	GAWTC	4 cut(s)	850, 883, 1302, 1441
PflMI	CCANNNNNTGG	1 cut(s)	1824
PinAI	ACCGGT	2 cut(s)	434, 1513
PkrI	GCNGC	8 cut(s)	31, 34, 102, 941, 968, 1005, 1060, 1178
PleI	GAGTC	1 cut(s)	1672
PpsI	GAGTC	1 cut(s)	1672
Ppu21I	YACGTR	1 cut(s)	868
Psp6I	CCWGG	2 cut(s)	583, 699
PspFI	CCCAGC	2 cut(s)	1186, 1233
PspGI	CCWGG	2 cut(s)	583, 699
PspN4I	GGNNCC	5 cut(s)	181, 294, 873, 1528, 1570
PspPI	GGNCC	3 cut(s)	206, 674, 1640
PspXI	VCTCGAGB	1 cut(s)	767
PsuI	RGATCY	3 cut(s)	518, 871, 1668
PvuII	CAGCTG	1 cut(s)	1061
RruI	TCGCGA	1 cut(s)	832
RsaI	GTAC	4 cut(s)	82, 665, 1295, 1539
RsaNI	GTAC	4 cut(s)	81, 664, 1294, 1538
RseI	CAYNNNNRTG	1 cut(s)	1739
SaqAI	TTAA	2 cut(s)	123, 948
SatI	GCNGC	8 cut(s)	30, 33, 101, 940, 967, 1004, 1059, 1177
Sau3AI	GATC	5 cut(s)	174, 518, 721, 871, 1668
Sau96I	GGNCC	3 cut(s)	206, 674, 1640
ScaI	AGTACT	1 cut(s)	1539
SchI	GAGTC	1 cut(s)	1672
ScrFI	CCNGG	2 cut(s)	585, 701
SduI	GDGCHC	4 cut(s)	295, 1423, 1714, 1817
SfaNI	GCATC	4 cut(s)	481, 501, 659, 1122
Sfr274I	CTCGAG	1 cut(s)	767
SfuI	TTCGAA	1 cut(s)	1081
SinI	GGWCC	2 cut(s)	674, 1640
SlaI	CTCGAG	1 cut(s)	767
SmiMI	CAYNNNNRTG	1 cut(s)	1739
SmlI	CTYRAG	2 cut(s)	767, 1346
SmoI	CTYRAG	2 cut(s)	767, 1346
SpeI	ACTAGT	1 cut(s)	1193
SseBI	AGGCCT	1 cut(s)	1386
SsiI	CCGC	1 cut(s)	1227
SspMI	CTAG	6 cut(s)	224, 329, 350, 1137, 1194, 1535
StuI	AGGCCT	1 cut(s)	1386
StyD4I	CCNGG	2 cut(s)	583, 699
TaaI	ACNGT	3 cut(s)	187, 429, 528
TaiI	ACGT	3 cut(s)	235, 861, 870
TaqI	TCGA	5 cut(s)	624, 768, 1081, 1664, 1772
TatI	WGTACW	2 cut(s)	663, 1537
TfiI	GAWTC	4 cut(s)	850, 883, 1302, 1441
Tru1I	TTAA	2 cut(s)	123, 948
Tru9I	TTAA	2 cut(s)	123, 948
TscAI	CASTG	6 cut(s)	190, 489, 697, 901, 1758, 1792
TseFI	GTSAC	2 cut(s)	480, 799
TseI	GCWGC	8 cut(s)	29, 32, 100, 939, 966, 1003, 1058, 1176
Tsp45I	GTSAC	2 cut(s)	480, 799
TspDTI	ATGAA	3 cut(s)	240, 890, 1392
TspGWI	ACGGA	2 cut(s)	1434, 1561
TspRI	CASTG	6 cut(s)	190, 489, 697, 901, 1758, 1792
Van91I	CCANNNNNTGG	1 cut(s)	1824
VneI	GTGCAC	1 cut(s)	1813
VpaK11BI	GGWCC	2 cut(s)	674, 1640
XapI	RAATTY	2 cut(s)	119, 1341
XceI	RCATGY	1 cut(s)	169
XcmI	CCANNNNNNNNNTGG	3 cut(s)	216, 1304, 1468
XhoI	CTCGAG	1 cut(s)	767
XmiI	GTMKAC	1 cut(s)	1611
XmnI	GAANNNNTTC	1 cut(s)	1670
XspI	CTAG	6 cut(s)	224, 329, 350, 1137, 1194, 1535
ZraI	GACGTC	1 cut(s)	233
ZrmI	AGTACT	1 cut(s)	1539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.