Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0002428

Physical Location & Seq

Genomic Coordinates Forward (+)

29560196 .. 29567002

Gene Structure 6807 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1245300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4434 bp

ATGCTGAAATCCTTGACGGATAAATGTGATAGGAAGGAAAAAGGCAAACAGGTCATTTTTATAAAAGATGAAGCAAATCAAACAAATGGTGACGAACTCCTGCAGAACATTGTGAAGTCATCATCTGCAGCCATTTCTAAACCTTACAACAAAAACAAAGAGTCTGACAGTGGTGGTGACAGAGGGATTGATCTGAATAAGACACCCCAGCAGAAACCACCAAAGCGAAGAAAACACAGGCCAAAGGTAATCATTGAAGGCAAGCCCAAGAGGACGCCAAAGCCTGCAACTCCAAAGAATACAAAGTCTAAGGAGACCAGACCTGCAAAGAGGAAGTTTGAAAGAATGAACGTCCAAAAGGAATCGCCAAGTCAACTGGCAGACATAACACGGGAGACGGCAGACGCTAATGTTGGAAAAGGTGCAAAATCATGCAGAAGAGTGCTGCATTTTGATTTGGAAAAGACCATGGATGAAAACCTATGCAGAGCAATTGGTCAGCAAGAGGAGACACAACAAGTGAACAAGAAGACTTTTGATTTGAATTTTGACTCTCAAGGGACACATATGGGAACTGAAACGAACCAGGTGAATGCAAAGCCAGCTGAGCGAAGTGGACTGCTGAACGAATTGTTGGTAGATAAGCAGATACCAGGTACTCTGAGTAACCCTATCCATTCCATGAGTCTTATGCCAAATAACTTTACATTTCTGCCAGAAAGGCAAGTATCTGCAGCCCTACTTGCTACAACAAAAGACATGCATCTGAAAAATTCGCATGTCATGAGGAGACTTGTAGAGAATGCAAATTCTGATTTATGCCAGAAAAGATGCAGAGATGAATCCACTCCAATGCAGCAACATAGCCATGCTGGAGAAATATGCCAAGATGTCATTCGAGCAAACATTAACCATGAAAACCTTCAGAAAACTAAAGAACTTGTCAACCAGGGTGATTCTTTACTAAAACTTCTCTCCCTTCCAAGTGAAGGAAGGGGATCCAAGAGGGATTTTTTTCGAACTGCTGAGGGCACTCGCCTTTCTACCAACAATCCACCAAGTTCACTATCATTCCATGAGTTACTTCAATTGGATGAAATTCAGAAAAACAGTGGTATTCTCAACAAAGATTTTTCAGGAAGCCGTAAGAAAAAGAAAATTGAGAATAGATACTTGAACATTAATAATATGCCTAATAAAGTTACAGCGGTTGAGGAGTGTCTGAGAAAAGTTGAAAGACCAGAGGAAAATAATGTCAATTCAAATGGATTTACATCGAAGAACCATAAAATGCTGAGTTCTTACATTAAAAATAACAAAATTATGGACAGGGAGAACAAAGAGATCAGCAAGTTTATAGGTGACAGGCCAAAGCACAACAATTGGTATCAGAGCTTTGTTGGAAGCTTTGGTTCATCGAAGATGAGAGTAAAGGATGTATTGACTCAACAAGGCTTGGCTAGAGCTTTGAAGGGAAAAGATGGGAAGCCTCAAGAAATGACAGATGACCAGTGGGAGGAGCTGGAGGGGCATTGTGTTAGCACGATTCGACACTGTATAGCTGATAACATTATCAATAATGTTATGGATGAAGATTATGAGCCTGCACTTTGGGAGAAGTTGGAAAAACTCTATATTGCTAAGAGTTTGACTAACAAGTTGCATTTGAAGCGGAAACTGTACAAGCTGAAGATGGATGAAGGTGGGAATCTCATGGACCACATGAATGTGTTCAACGGATACTTGGATCAATTGAGGAAAGTTGATGTCAAGGTTGAGGAAGAAGACAAAGCCCTCTTAGTTCTTACCTCACTTCCAGATTCGTATGAAAATCTTGTGACAACCTTACTGCATGGAAAAGATACAGTAAGTTTGGAGCAGGTGCAAGCTTCTTTGGTGTCTTATGATACACAAAAGAAGAAGACCATTGTTGATGGTGGGCACGAGACTGCTTTAGCTGTTCAAGGTTGGAATCGTGGAAGAAAATTGGGAGAAGGATCTGAGAGAGACAACATGTTCAAATCCGGTTCCGGAGGCAAGGGTCTACAATGCTACGCCTGTAAAGAATTTGGGCATAAAAAGATAAATTGTCCTTTGAGAAAAAGAAAGGATGATCTTGGTCCGAGCAGTAGCAATTTTGTGGAAGAAGACTTCGAGCCAAAGCACAACAACAGGTACACTCACTCCATCACTTCTGGGAATGGTTTTGTACATCTGCATATCTCATCCAAATCAAATTCATGCCAAGGTTCCACAAAAGTTCTTAGCTTTTCCACCCATTCAACAAGTGAAACATGTAACCAGCAAGCCTCATCACTTCCCAGAAAATCCCTTCAGATAGCAAACAAGCAGGTATCTCATGATAATACGTCAGCAAAGAAGCAGTCTGAAGGAATTACTCTATCGAAAGTTTCATCAGGTACAGATAAAGTCTGTCAAGAGAAGGATTCAGCGTATGATCACAAGCAACCTTCTGCAAAAGCAATTGGGTATCCAATAAGAACAAGATGTACTATACCAATAGATGGCATCATACATCAATTTAATGGTCTGAACCTCAATGGAAGCTGCAGCAAAATTCTAGAGCAGGAGAAAGCTGCACTTGTTCCTTACAAAGGAGATGGTGTAATTGTCCCCTTTCAAGGTATTATAAAGAAACATAAGCCGCGACCAAGAGTAGAGCTTGACCCAGAAACATATAGGATATGGAATCTGTTGATGGGCAATGAGGGAAGCAAAGGTAATGAAGGAACTGATAAAAATGAGAAGTATTGGGAAGAGGAAAGAAATGTTTTCCAAGGACGAGTTGACTCATTCATTGCGCGCATGCACCTTGTTCAAGACAGACGTTTTTCAAAATGGAAAGGATCAGTTGTTGACTCGGTGATCGGAGTTTTCCTTACCCAGAATGTCTCAGACCATCTATCAAGCTCTGCCTTCATGTCATTGGCAGCACGGTTTCCGCTTCAGTCAAGCAATCACCAAGCTCCACACAAGGTTCGGATAAACATCTTGGTTAACAAACCAGAAGTTTGCATGACAAGTCCAGATGATGCCACCAAATGGCATAAAGATATATCAAGTCAACCAATCTACCGCCAGACTTCTATGACACTCCATGAATTGGCAGAAAATCAGAGGGACATTGAGAACTCATGGACAGAAAGGAACTTAGATGAGGAACATGGTCAATGTTTTGAGGAAGAATTTGTATCGTCTCAAGATTCTATTGAATCCTCAGTCACTCAAGGAGCTGTAGGAATCAGATCATACCCAATGTCCAACTCAGAAACAGAAGATCCTATGACTGGATGCCGACCCAACAAGGTTCCTATTTCCATTTCAACCTATCAACAGATGGAGACGACGACCATGTTTCAGGACTTTTACCGTCAAGTAAATGGAATCTCACTATTAGATGATGGATCCAAGAATGGGCACATGGAGTATTCACAGATAAAGCCACCATCAGACAAAATCAATCATCTCACTGGCACGTCTTTCACAAATCCAATCAATCTTGACAATGAAAATATGCAAGTACCAGTTGTTCCTTCCAGCAACAATCAATTGCACATGTACCCAGATTCTGGAGAACCAAGGCCATGGAGATTTGGAACTTTCAATGAGGAGAGCATATCCTCTTGGCCTTCGACTGCTTCCAGATTCGATATTGAGCAAGACAAGTGCAAGAACTTGAGAAATGAAGAACTGTTAGGAAGTGTTGTTAATTCCTCTATGCAACAGAGTAGTCTGTGGAGTTCTCAAGAAATGCCACCAGTGGACCCGTATGCATTGTTCGGACAGCACTCAACAGACCTGAAAAATAGGTCTGAAACAAGGCCAAGCACTGGACATAATCGTTCCAACTATAGTCATCAGCGTGAGAGGAACCTAACCTTCTCATTAGAAAACACGTCAGCCAGAGAGCCTGGAAAACATACTGAACCACTTATGGGAAATAAGAGTGGTAGCATGCAGCATGTCAATAATTTCAGTGAGCTCAGAAAAAAATCTATCAATGTCGTTGATGGTATGCATGTACACATGGAAAATCAACCAGTTGATTCAAATTTACAGGAGCAACAATCTTCTCATGGTCAGTCACATAAGGAGACAAGTACAAATACTTCAAAAAGAAGAAAAGGAAAGGCTTACAGCGAGAAAAAGAATGCAGTTGACTGGGACATTTTGAGAAACCAGGTCTTGGCCAAGGGTAGGGAAAAGGAAAGAAACAAAGACACAATGGACTCACTGGATTATGAAGCACTAAAAAATGCTAATGTCAGAGAAATTTCTGAGGCAATCAAGGAGCGAGGGATGAACATCATGCTAGCAGAACAGATCCAGGAATTTCTAAACCGACTGGTTAAAGAACATGGAAGCATCGATCTGGAATGGTTAAGAGATGTTCCCCCTGATAAAGCAAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010468 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019222 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043076 GO:0043078 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Perm-CXXC RRM_DME

Protein Analysis

1478

Amino Acids

167.24

Weight (kDa)

8.81

Isoelectric Point (pI)

46.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Retrotran_gag_2	PF14223	480 - 641	4.4e-29	gag-polypeptide of LTR copia-type

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04560 AT5G04560 AT5G04560
fragaria_vesca	FvH4_2g10170 FvH4_2g10170 FvH4_2g10170 FvH4_2g10170 FvH4_2g10170
malus_domestica	MD02G1245300.v1.1 MD07G1071800.v1.1 MD09G1259000.v1.1 MD09G1259100.v1.1 MD17G1253500.v1.1 MD17G1253600.v1.1
prunus_persica	Prupe.2G089400_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1
pyrus_communis	pycom02g21110 pycom09g17380 pycom17g25620 pycom17g25630
rosa_chinensis	RchiOBHm_Chr6g0267351 RchiOBHm_Chr6g0267361
rosa_laevigata	RLG00000014013
rosa_multiflora	Rmu_sc0000746.1_g000002 Rmu_sc0019664.1_g000001
rosa_roxburghii	Rroxscaffold_7G00200830
rosa_rugosa	Rorug06G0031600 Rorug06G0031700 Rorug06G0031800 Rorug06G0031900 Rorug06G0032000
rosa_samantha	Rh6AG153500 Rh6BG153200 Rh6CG149300 Rh6DG137600
rosa_wichuraiana	Rw6G013200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	62, 2651
AarI	CACCTGC	1 cut(s)	1870
Acc36I	ACCTGC	3 cut(s)	331, 1870, 2341
AccB7I	CCANNNNNTGG	6 cut(s)	2525, 3063, 3124, 3590, 3851, 4207
AccI	GTMKAC	1 cut(s)	2044
AccII	CGCG	2 cut(s)	2668, 2824
AccIII	TCCGGA	1 cut(s)	2030
AciI	CCGC	5 cut(s)	1208, 1672, 2666, 2963, 3097
AclWI	GGATC	9 cut(s)	993, 1006, 1755, 2005, 2875, 3293, 3420, 3433, 4339
AcoI	YGGCCR	1 cut(s)	4209
AcuI	CTGAAG	5 cut(s)	908, 1709, 2318, 2409, 2951
AcyI	GRCGYC	1 cut(s)	275
AflIII	ACRYGT	4 cut(s)	2013, 2294, 3576, 3915
AjiI	CACGTC	2 cut(s)	3498, 3918
AjnI	CCWGG	6 cut(s)	585, 652, 948, 3931, 4200, 4347
AjuI	GAANNNNNNNTTGG	4 cut(s)	617, 649, 879, 911
Alw21I	GWGCWC	1 cut(s)	4005
AlwI	GGATC	9 cut(s)	993, 1006, 1755, 2005, 2875, 3293, 3420, 3433, 4339
AlwNI	CAGNNNCTG	1 cut(s)	3590
Aor13HI	TCCGGA	1 cut(s)	2030
AoxI	GGCC	6 cut(s)	239, 1367, 3602, 3647, 3842, 4209
ApeKI	GCWGC	9 cut(s)	128, 445, 734, 856, 2568, 2571, 2597, 2951, 3979
ArsI	GACNNNNNNTTYG	4 cut(s)	523, 555, 2369, 2401
AseI	ATTAAT	1 cut(s)	1182
Asp700I	GAANNNNTTC	2 cut(s)	921, 1730
AspLEI	GCGC	2 cut(s)	2824, 2826
AspS9I	GGNCC	3 cut(s)	1717, 2120, 3784
AsuHPI	GGTGA	7 cut(s)	101, 188, 601, 965, 1373, 2896, 2972
AsuII	TTCGAA	1 cut(s)	1018
AsuNHI	GCTAGC	1 cut(s)	4333
AvaII	GGWCC	3 cut(s)	1717, 2120, 3784
BaeGI	GKGCMC	3 cut(s)	1034, 1944, 3441
BalI	TGGCCA	1 cut(s)	4211
BamHI	GGATCC	2 cut(s)	998, 3425
BanII	GRGCYC	1 cut(s)	4005
BarI	GAAGNNNNNNTAC	2 cut(s)	4114, 4146
BauI	CACGAG	1 cut(s)	1943
BbsI	GAAGAC	4 cut(s)	536, 1791, 1928, 2154
Bbv12I	GWGCWC	1 cut(s)	4005
BbvCI	CCTCAGC	1 cut(s)	1026
BbvI	GCAGC	9 cut(s)	140, 432, 746, 868, 2555, 2583, 2584, 2963, 3991
BceAI	ACGGC	2 cut(s)	414, 1128
BciT130I	CCWGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
BciVI	GTATCC	2 cut(s)	1733, 2502
BclI	TGATCA	1 cut(s)	2458
BfaI	CTAG	3 cut(s)	1461, 2582, 4334
BfmI	CTRYAG	6 cut(s)	101, 126, 732, 2569, 3255, 3871
BfuAI	ACCTGC	3 cut(s)	331, 1870, 2341
BfuI	GTATCC	2 cut(s)	1733, 2502
BglI	GCCNNNNNGGC	1 cut(s)	721
BlpI	GCTNAGC	1 cut(s)	606
Bme1390I	CCNGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
Bme18I	GGWCC	3 cut(s)	1717, 2120, 3784
BmgBI	CACGTC	2 cut(s)	3498, 3918
BmgT120I	GGNCC	3 cut(s)	1717, 2120, 3784
BmiI	GGNNCC	7 cut(s)	1000, 2029, 2251, 3330, 3427, 3786, 3893
BmrFI	CCNGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
BmrI	ACTGGG	1 cut(s)	4192
BmsI	GCATC	6 cut(s)	772, 821, 2538, 3043, 3302, 4395
BmtI	GCTAGC	1 cut(s)	4337
BmuI	ACTGGG	1 cut(s)	4192
BpiI	GAAGAC	4 cut(s)	536, 1791, 1928, 2154
BpmI	CTGGAG	3 cut(s)	894, 1544, 3612
Bpu10I	CCTNAGC	1 cut(s)	1026
Bpu1102I	GCTNAGC	1 cut(s)	606
Bpu14I	TTCGAA	1 cut(s)	1018
BpuEI	CTTGAG	6 cut(s)	540, 1476, 3204, 3231, 3718, 3750
Bsa29I	ATCGAT	1 cut(s)	4389
BsaBI	GATNNNNATC	3 cut(s)	1273, 3008, 4325
BsaHI	GRCGYC	1 cut(s)	275
BsaI	GGTCTC	1 cut(s)	308
BsaJI	CCNNGG	7 cut(s)	468, 949, 2245, 2797, 3599, 3605, 4212
BsaWI	WCCGGW	2 cut(s)	2024, 2030
BsaXI	ACNNNNNCTCC	4 cut(s)	2168, 2198, 2610, 2640
Bse3DI	GCAATG	2 cut(s)	2731, 2817
Bse8I	GATNNNNATC	3 cut(s)	1273, 3008, 4325
BseAI	TCCGGA	1 cut(s)	2030
BseBI	CCWGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
BseCI	ATCGAT	1 cut(s)	4389
BseDI	CCNNGG	7 cut(s)	468, 949, 2245, 2797, 3599, 3605, 4212
BseGI	GGATG	9 cut(s)	478, 1099, 1441, 1594, 1702, 2116, 2225, 3317, 4326
BseJI	GATNNNNATC	3 cut(s)	1273, 3008, 4325
BseMI	GCAATG	2 cut(s)	2731, 2817
BsePI	GCGCGC	1 cut(s)	2822
BseRI	GAGGAG	5 cut(s)	521, 802, 1229, 1532, 3644
BseSI	GKGCMC	3 cut(s)	1034, 1944, 3441
BseXI	GCAGC	9 cut(s)	140, 432, 746, 868, 2555, 2583, 2584, 2963, 3991
BseYI	CCCAGC	1 cut(s)	207
BsgI	GTGCAG	2 cut(s)	1590, 2583
Bsh1236I	CGCG	2 cut(s)	2668, 2824
BshFI	GGCC	6 cut(s)	241, 1369, 3604, 3649, 3844, 4211
BshVI	ATCGAT	1 cut(s)	4389
BsiHKAI	GWGCWC	1 cut(s)	4005
BsiSI	CCGG	2 cut(s)	2025, 2031
BslFI	GGGAC	4 cut(s)	574, 2618, 3155, 4199
BsmBI	CGTCTC	3 cut(s)	389, 3222, 3356
BsmFI	GGGAC	4 cut(s)	574, 2618, 3155, 4199
BsmI	GAATGC	3 cut(s)	598, 808, 4177
BsnI	GGCC	6 cut(s)	241, 1369, 3604, 3649, 3844, 4211
Bso31I	GGTCTC	1 cut(s)	308
Bsp119I	TTCGAA	1 cut(s)	1018
Bsp1286I	GDGCHC	4 cut(s)	1034, 1944, 3441, 4005
Bsp13I	TCCGGA	1 cut(s)	2030
Bsp1407I	TGTACA	3 cut(s)	1680, 2209, 4042
Bsp1720I	GCTNAGC	1 cut(s)	606
Bsp19I	CCATGG	2 cut(s)	468, 3605
BspACI	CCGC	5 cut(s)	1208, 1672, 2666, 2963, 3097
BspANI	GGCC	6 cut(s)	241, 1369, 3604, 3649, 3844, 4211
BspDI	ATCGAT	1 cut(s)	4389
BspEI	TCCGGA	1 cut(s)	2030
BspFNI	CGCG	2 cut(s)	2668, 2824
BspHI	TCATGA	2 cut(s)	783, 2359
BspLI	GGNNCC	7 cut(s)	1000, 2029, 2251, 3330, 3427, 3786, 3893
BspMAI	CTGCAG	4 cut(s)	105, 130, 736, 2573
BspMI	ACCTGC	3 cut(s)	331, 1870, 2341
BspOI	GCTAGC	1 cut(s)	4337
BspPI	GGATC	9 cut(s)	993, 1006, 1755, 2005, 2875, 3293, 3420, 3433, 4339
BspT104I	TTCGAA	1 cut(s)	1018
BspTNI	GGTCTC	1 cut(s)	308
BsrDI	GCAATG	2 cut(s)	2731, 2817
BsrGI	TGTACA	3 cut(s)	1680, 2209, 4042
BssECI	CCNNGG	7 cut(s)	468, 949, 2245, 2797, 3599, 3605, 4212
BssHII	GCGCGC	1 cut(s)	2822
BssNI	GRCGYC	1 cut(s)	275
BssSI	CACGAG	1 cut(s)	1943
BssT1I	CCWWGG	6 cut(s)	468, 2245, 2797, 3599, 3605, 4212
Bst2BI	CACGAG	1 cut(s)	1943
Bst2UI	CCWGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
Bst4CI	ACNGT	8 cut(s)	170, 1112, 1556, 1680, 1867, 2958, 3392, 3714
Bst6I	CTCTTC	2 cut(s)	433, 2772
BstACI	GRCGYC	1 cut(s)	275
BstAUI	TGTACA	3 cut(s)	1680, 2209, 4042
BstBI	TTCGAA	1 cut(s)	1018
BstDSI	CCRYGG	2 cut(s)	468, 3605
BstENI	CCTNNNNNAGG	1 cut(s)	2613
BstF5I	GGATG	9 cut(s)	478, 1099, 1441, 1594, 1702, 2116, 2225, 3317, 4326
BstFNI	CGCG	2 cut(s)	2668, 2824
BstHHI	GCGC	2 cut(s)	2824, 2826
BstMWI	GCNNNNNNNGC	6 cut(s)	602, 607, 721, 743, 1528, 1669
BstNI	CCWGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
BstNSI	RCATGY	9 cut(s)	763, 782, 2017, 2298, 2830, 3580, 3979, 3986, 4043
BstSCI	CCNGG	6 cut(s)	585, 652, 948, 3931, 4200, 4347
BstSFI	CTRYAG	6 cut(s)	101, 126, 732, 2569, 3255, 3871
BstSLI	GKGCMC	3 cut(s)	1034, 1944, 3441
BstUI	CGCG	2 cut(s)	2668, 2824
BstV1I	GCAGC	9 cut(s)	140, 432, 746, 868, 2555, 2583, 2584, 2963, 3991
BstV2I	GAAGAC	4 cut(s)	536, 1791, 1928, 2154
BstX2I	RGATCY	5 cut(s)	998, 1997, 3298, 3425, 4344
BstXI	CCANNNNNNTGG	1 cut(s)	3606
BstYI	RGATCY	5 cut(s)	998, 1997, 3298, 3425, 4344
Bsu15I	ATCGAT	1 cut(s)	4389
BsuI	GTATCC	2 cut(s)	1733, 2502
BsuRI	GGCC	6 cut(s)	241, 1369, 3604, 3649, 3844, 4211
BsuTUI	ATCGAT	1 cut(s)	4389
BtgI	CCRYGG	2 cut(s)	468, 3605
BtrI	CACGTC	2 cut(s)	3498, 3918
BtsCI	GGATG	9 cut(s)	478, 1099, 1441, 1594, 1702, 2116, 2225, 3317, 4326
BtsIMutI	CAGTG	9 cut(s)	175, 1117, 1517, 1552, 3489, 3786, 3849, 4003, 4253
BveI	ACCTGC	3 cut(s)	331, 1870, 2341
CaiI	CAGNNNCTG	1 cut(s)	3590
CciI	TCATGA	2 cut(s)	783, 2359
CfoI	GCGC	2 cut(s)	2824, 2826
Cfr13I	GGNCC	3 cut(s)	1717, 2120, 3784
ClaI	ATCGAT	1 cut(s)	4389
CseI	GACGC	2 cut(s)	283, 413
CsiI	ACCWGGT	3 cut(s)	585, 652, 4200
EaeI	YGGCCR	1 cut(s)	4209
Eam1104I	CTCTTC	2 cut(s)	433, 2772
EarI	CTCTTC	2 cut(s)	433, 2772
Ecl136II	GAGCTC	1 cut(s)	4003
Eco130I	CCWWGG	6 cut(s)	468, 2245, 2797, 3599, 3605, 4212
Eco24I	GRGCYC	1 cut(s)	4005
Eco31I	GGTCTC	1 cut(s)	308
Eco47I	GGWCC	3 cut(s)	1717, 2120, 3784
Eco53kI	GAGCTC	1 cut(s)	4003
Eco57I	CTGAAG	5 cut(s)	908, 1709, 2318, 2409, 2951
EcoICRI	GAGCTC	1 cut(s)	4003
EcoNI	CCTNNNNNAGG	1 cut(s)	2613
EcoRII	CCWGG	6 cut(s)	585, 652, 948, 3931, 4200, 4347
EcoT14I	CCWWGG	6 cut(s)	468, 2245, 2797, 3599, 3605, 4212
EcoT22I	ATGCAT	3 cut(s)	765, 3796, 4041
EcoT38I	GRGCYC	1 cut(s)	4005
ErhI	CCWWGG	6 cut(s)	468, 2245, 2797, 3599, 3605, 4212
Esp3I	CGTCTC	3 cut(s)	389, 3222, 3356
FalI	AAGNNNNNCTT	2 cut(s)	2586, 2618
FaqI	GGGAC	4 cut(s)	574, 2618, 3155, 4199
FauNDI	CATATG	1 cut(s)	567
FbaI	TGATCA	1 cut(s)	2458
FblI	GTMKAC	1 cut(s)	2044
FokI	GGATG	9 cut(s)	485, 1106, 1448, 1601, 1709, 2123, 2212, 3324, 4333
FriOI	GRGCYC	1 cut(s)	4005
FspBI	CTAG	3 cut(s)	1461, 2582, 4334
GlaI	GCGC	2 cut(s)	2823, 2825
GsaI	CCCAGC	1 cut(s)	211
GsuI	CTGGAG	3 cut(s)	894, 1544, 3612
HaeIII	GGCC	6 cut(s)	241, 1369, 3604, 3649, 3844, 4211
HapII	CCGG	2 cut(s)	2025, 2031
HgaI	GACGC	2 cut(s)	283, 413
HhaI	GCGC	2 cut(s)	2824, 2826
Hin1I	GRCGYC	1 cut(s)	275
Hin6I	GCGC	2 cut(s)	2822, 2824
HinP1I	GCGC	2 cut(s)	2822, 2824
HincII	GTYRAC	7 cut(s)	374, 946, 2809, 2878, 3019, 3086, 4180
HindII	GTYRAC	7 cut(s)	374, 946, 2809, 2878, 3019, 3086, 4180
HindIII	AAGCTT	2 cut(s)	1405, 1887
HpaI	GTTAAC	1 cut(s)	3019
HpaII	CCGG	2 cut(s)	2025, 2031
HphI	GGTGA	7 cut(s)	101, 188, 601, 965, 1373, 2896, 2972
Hpy99I	CGWCG	1 cut(s)	3370
HpyCH4III	ACNGT	8 cut(s)	170, 1112, 1556, 1680, 1867, 2958, 3392, 3714
HpyCH4IV	ACGT	5 cut(s)	351, 2369, 2848, 3497, 3917
HpyF10VI	GCNNNNNNNGC	6 cut(s)	602, 607, 721, 743, 1528, 1669
HpySE526I	ACGT	5 cut(s)	351, 2369, 2848, 3497, 3917
Hsp92I	GRCGYC	1 cut(s)	275
HspAI	GCGC	2 cut(s)	2822, 2824
Kpn2I	TCCGGA	1 cut(s)	2030
Ksp22I	TGATCA	1 cut(s)	2458
KspAI	GTTAAC	1 cut(s)	3019
LmnI	GCTCC	6 cut(s)	1519, 1876, 2992, 3251, 4081, 4312
Lsp1109I	GCAGC	9 cut(s)	140, 432, 746, 868, 2555, 2583, 2584, 2963, 3991
LweI	GCATC	6 cut(s)	772, 821, 2538, 3043, 3302, 4395
MabI	ACCWGGT	3 cut(s)	585, 652, 4200
MaeI	CTAG	3 cut(s)	1461, 2582, 4334
MaeII	ACGT	5 cut(s)	351, 2369, 2848, 3497, 3917
MfeI	CAATTG	6 cut(s)	492, 1088, 1381, 1751, 2484, 3569
MflI	RGATCY	5 cut(s)	998, 1997, 3298, 3425, 4344
MhlI	GDGCHC	4 cut(s)	1034, 1944, 3441, 4005
MlsI	TGGCCA	1 cut(s)	4211
MluNI	TGGCCA	1 cut(s)	4211
MlyI	GAGTC	7 cut(s)	170, 545, 694, 1438, 2804, 2873, 4244
MmeI	TCCRAC	6 cut(s)	394, 1381, 1602, 1949, 3306, 3891
Mox20I	TGGCCA	1 cut(s)	4211
Mph1103I	ATGCAT	3 cut(s)	765, 3796, 4041
MroI	TCCGGA	1 cut(s)	2030
MroXI	GAANNNNTTC	2 cut(s)	921, 1730
MscI	TGGCCA	1 cut(s)	4211
MseI	TTAA	8 cut(s)	909, 1182, 1308, 2544, 3018, 3729, 4371, 4403
MslI	CAYNNNNRTG	5 cut(s)	851, 867, 1725, 1727, 3882
Msp20I	TGGCCA	1 cut(s)	4211
MspA1I	CMGCKG	2 cut(s)	605, 1208
MspI	CCGG	2 cut(s)	2025, 2031
MspR9I	CCNGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
MunI	CAATTG	6 cut(s)	492, 1088, 1381, 1751, 2484, 3569
Mva1269I	GAATGC	3 cut(s)	598, 808, 4177
MvaI	CCWGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
MvnI	CGCG	2 cut(s)	2668, 2824
MwoI	GCNNNNNNNGC	6 cut(s)	602, 607, 721, 743, 1528, 1669
NcoI	CCATGG	2 cut(s)	468, 3605
NdeI	CATATG	1 cut(s)	567
NheI	GCTAGC	1 cut(s)	4333
NlaIV	GGNNCC	7 cut(s)	1000, 2029, 2251, 3330, 3427, 3786, 3893
NmuCI	GTSAC	6 cut(s)	89, 176, 1361, 1837, 3241, 4104
NsiI	ATGCAT	3 cut(s)	765, 3796, 4041
NspI	RCATGY	9 cut(s)	763, 782, 2017, 2298, 2830, 3580, 3979, 3986, 4043
NspV	TTCGAA	1 cut(s)	1018
PaeI	GCATGC	2 cut(s)	2830, 3979
PagI	TCATGA	2 cut(s)	783, 2359
PaqCI	CACCTGC	1 cut(s)	1870
PauI	GCGCGC	1 cut(s)	2822
PciI	ACATGT	3 cut(s)	2013, 2294, 3576
PctI	GAATGC	3 cut(s)	598, 808, 4177
PdmI	GAANNNNTTC	2 cut(s)	921, 1730
PflMI	CCANNNNNTGG	6 cut(s)	2525, 3063, 3124, 3590, 3851, 4207
PfoI	TCCNGGA	1 cut(s)	4347
PleI	GAGTC	7 cut(s)	169, 545, 693, 1438, 2804, 2873, 4244
PpsI	GAGTC	7 cut(s)	169, 545, 693, 1438, 2804, 2873, 4244
PscI	ACATGT	3 cut(s)	2013, 2294, 3576
PshBI	ATTAAT	1 cut(s)	1182
PsiI	TTATAA	2 cut(s)	62, 2651
Psp124BI	GAGCTC	1 cut(s)	4005
Psp6I	CCWGG	6 cut(s)	585, 652, 948, 3931, 4200, 4347
PspFI	CCCAGC	1 cut(s)	207
PspGI	CCWGG	6 cut(s)	585, 652, 948, 3931, 4200, 4347
PspN4I	GGNNCC	7 cut(s)	1000, 2029, 2251, 3330, 3427, 3786, 3893
PspPI	GGNCC	3 cut(s)	1717, 2120, 3784
PsrI	GAACNNNNNNTAC	6 cut(s)	2494, 2526, 3534, 3566, 3782, 3814
PstI	CTGCAG	4 cut(s)	105, 130, 736, 2573
PstNI	CAGNNNCTG	1 cut(s)	3590
PsuI	RGATCY	5 cut(s)	998, 1997, 3298, 3425, 4344
PteI	GCGCGC	1 cut(s)	2822
PvuII	CAGCTG	1 cut(s)	605
RseI	CAYNNNNRTG	5 cut(s)	851, 867, 1725, 1727, 3882
SacI	GAGCTC	1 cut(s)	4005
SaqAI	TTAA	8 cut(s)	909, 1182, 1308, 2544, 3018, 3729, 4371, 4403
Sau96I	GGNCC	3 cut(s)	1717, 2120, 3784
SchI	GAGTC	7 cut(s)	170, 545, 694, 1438, 2804, 2873, 4244
ScrFI	CCNGG	6 cut(s)	587, 654, 950, 3933, 4202, 4349
SduI	GDGCHC	4 cut(s)	1034, 1944, 3441, 4005
SexAI	ACCWGGT	3 cut(s)	585, 652, 4200
SfaNI	GCATC	6 cut(s)	772, 821, 2538, 3043, 3302, 4395
SfcI	CTRYAG	6 cut(s)	101, 126, 732, 2569, 3255, 3871
SfuI	TTCGAA	1 cut(s)	1018
SinI	GGWCC	3 cut(s)	1717, 2120, 3784
SmiMI	CAYNNNNRTG	5 cut(s)	851, 867, 1725, 1727, 3882
SmlI	CTYRAG	6 cut(s)	555, 1491, 3219, 3246, 3697, 3765
SmoI	CTYRAG	6 cut(s)	555, 1491, 3219, 3246, 3697, 3765
SphI	GCATGC	2 cut(s)	2830, 3979
SsiI	CCGC	5 cut(s)	1208, 1672, 2666, 2963, 3097
SspMI	CTAG	3 cut(s)	1461, 2582, 4334
SstI	GAGCTC	1 cut(s)	4005
StyD4I	CCNGG	6 cut(s)	585, 652, 948, 3931, 4200, 4347
StyI	CCWWGG	6 cut(s)	468, 2245, 2797, 3599, 3605, 4212
TaaI	ACNGT	8 cut(s)	170, 1112, 1556, 1680, 1867, 2958, 3392, 3714
TaiI	ACGT	5 cut(s)	354, 2372, 2851, 3500, 3920
TatI	WGTACW	5 cut(s)	1680, 2209, 2510, 4042, 4121
TauI	GCSGC	1 cut(s)	2668
Tru1I	TTAA	8 cut(s)	909, 1182, 1308, 2544, 3018, 3729, 4371, 4403
Tru9I	TTAA	8 cut(s)	909, 1182, 1308, 2544, 3018, 3729, 4371, 4403
TscAI	CASTG	9 cut(s)	175, 1117, 1517, 1559, 3496, 3786, 3856, 4003, 4260
TseFI	GTSAC	6 cut(s)	89, 176, 1361, 1837, 3241, 4104
TseI	GCWGC	9 cut(s)	128, 445, 734, 856, 2568, 2571, 2597, 2951, 3979
Tsp45I	GTSAC	6 cut(s)	89, 176, 1361, 1837, 3241, 4104
TspGWI	ACGGA	2 cut(s)	32, 1752
TspRI	CASTG	9 cut(s)	175, 1117, 1517, 1559, 3496, 3786, 3856, 4003, 4260
Van91I	CCANNNNNTGG	6 cut(s)	2525, 3063, 3124, 3590, 3851, 4207
VpaK11BI	GGWCC	3 cut(s)	1717, 2120, 3784
VspI	ATTAAT	1 cut(s)	1182
XagI	CCTNNNNNAGG	1 cut(s)	2613
XbaI	TCTAGA	1 cut(s)	2581
XceI	RCATGY	9 cut(s)	763, 782, 2017, 2298, 2830, 3580, 3979, 3986, 4043
XmiI	GTMKAC	1 cut(s)	2044
XmnI	GAANNNNTTC	2 cut(s)	921, 1730
XspI	CTAG	3 cut(s)	1461, 2582, 4334
Zsp2I	ATGCAT	3 cut(s)	765, 3796, 4041

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.