Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0002428

Physical Location & Seq

Genomic Coordinates Forward (+)

3816971 .. 3827259

Gene Structure 10289 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0032000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3267 bp

ATGAGCACAGAGAGCTCTTCACCTCCTCGGTGGAAATATGATGTCTTTTTGAGTTTTAGAGGCGTGGATACTCAAAAGGGTTTTACAGACCATCTATACACTGCGTTGGATGATAAAGGAATCACGACTTTCAGGGATGATCCTGAACTTCACAAAGGGGAAGCTATTTCTCCAGCACTCTTTGCTGCAATTGAAGAATCAAGATTTGCCCTCATCGTTCTCTCACAAAATTATGCATCGTTGACATGGTGCTTGAATGAACTTGTAAAAATTCTTGAATGCATGAAAGCAAGAGGAACTGTGCTGCCAATTTTTTATGATGTTGATCCCTCTGTTGTACGAAAGCAAACAGGGAGTTTTGGTGAAGCCTTTGCTAATCATGATGAAAGGTTTAGCGATGACAAGGAGAAAGTGTGGAGGTGGAGATGTGCTTTAACTGAAGTGGCAAGTTTCTCTGGGTGGAATTCAAAGGAATGGTATGAATCAAAGCTCATAAGAGTTATTGTTGAAGTTATATGGAAAAGATTGCAACATGAAGGGGTGGATGACGTCCGCTTCATTGGTATATGGGGAATGGGCGGGATTGGTAAGACAACTATGGTAAGAGCGGTGTATGCGAGAATCTCTCGTGAATTTGAATTTAGTTTCCTTCTTACTGATGTTAGAAAGTCCGTTGAAGAAAGTAGTCTACTTAAGTTACAAAAGCAACTTCTCTCTGGAATTTGGACAAAGAAGGCCAACTTATCAGACCCTCATGAAGGAGCCACCTCTATAAGGAGGTTGTTAGGTCACAAAAAAGTTCTTCTCGTTCTTGATGATGTGAACCATCCAAGCCATTTAAAATATTTGGCAGGAAACCAAGACTGGTTTGGTTCAGGGAGTAGAGTGCTTATCACAACTAGAAATGAGCATTTGTTGATTCAGCATGGAGTGGAGAGAAGATTGAAGGTCGAAGAATTAAATGATGATGATTCTCTTAAGCTTTTTAGCTGGAATGCATTCAAAAGAGGTTACCCTGAAGAAGATTTTCTTAATTGGTTTGTCAAAATCTGTTGTAAGGACGAAGATCGAGTAACAGAAGTACTAAACAGTTGCGATTTTTCTGCAATTATTGGAATACAAGTTCTTATCGAAAGATCTCTCTTGACTGTTTCGCATGGAAGACTAAGGATGCACGATTTGCTCCAAGAAATGGGATGGGATATTGTCCGCGGGGAATCTCCTAACGAGCCGGGCAAGTGGAGTAGGTTGTGGCTTCTTAAAGACGTTGAACATGTCTTGACCAAAAATACTGGAACTGAATCAATAGAAGGCATATTTCTGGACTCAACCAAGTTAGGAGTAGACATGGATGTGACTCCGAAATCATTTTCAATGATGAACAAATTGAGATACCTGAAGATTGAGAATGGGAACCTGCCTAAGGGACTTGAATTTCTTCCCAATAGTTTATGGATTCTTGATTGGACGAGGTATCCGTTAAAATCCCTGCCATCACATTTCAACCCTCAGAAGCTGCTCGAACTAGGCTTGTCTCATAGTTGTATTAAACATGTTCAGATAGGAACGGAGCCTTTATGCAACTTGAAAACCATTAATCTGAGTCAGTCTTTGAATCTTGTCAGCACCCCAAACTTTAAAGGTTGTATAAGATTGTATGAGGTTGACCCGACAATTGAAGTACTTGAAAGACTTACTGTGCTGAACTTGAAAGATTGCAAGAATCTTGTGCATTTTGCAAGCAGTGTAGGTGGCTTAAGTCTCTCAAAGGTTAACAAACTACCTAATGACACGTGTCATTTGGAGTGTTTGGAGGAGCTTTATCTGAATGGCACTGGCATAAAAGAACTACCTACGTCTACTGAAGTGCTTGAAAGACTTACTGTGCTGAACTTCAAAGACTGCAAGTATCTTGTGCGTCTTCCAAGCAGTGTAGGTGGCTTAAAATCTCTCAAATATCTCAATCTTTCTGGTTGCTCAAAGCTTGACCAATTGCCAAATGAGTTGGGTCCTGTTGCTTGTTTGGAGAAGCTTGATGTGAGTGGAACTGGCATAAGAGAAGTGCCCTCCTCTATTGGTCTTTTGAAAAACCTCAAAGAATTATCTCTTGCTGGATGTAAAGCACGGTCACCTAAATCATGGAATATGATGTTCGACCCCTTTCAGTTATTGCGAAAAAGAAGTCACATTCAGGCGGGATTGTCGTTGCCTTGTTTGTCTGGTTTGCATTCATTAACTGAATTGGATCCAAGTGACTGCAATCTTTCTGAAGACGCAATGCCCAGTGATTTTGGATGCTTGTCTTCATTAAAAAAATTCAATCTCAGCAGAAATCAATTTGTTAGACTACCTGAGAGCGTCGGCCAACTCTCTAGACTTCAATATCTTTACTTGAATTGGTGTTGCAAGCTACAAACATTACTAGAGCTTCCATCTCATGTATTTGTAAACGCTAGCGACTGCATTTCATTAGATACATTGGCCAATCAAATAAGACAATACTATTCGGTGCTAAAAGGAAGGTTTGTTAATTGTTTCAAAATGGTGGAGAATGAGAGCTGTAAGAGTATAGAACTTCCATTGCTAACCCGCTACCTTGAGAGTAAAGCATTTTCTGACCATGAAACATTTCAATTTGTTGGTCTTGGAAATGAAATTCCAGAGTGGTACAATCACCAAAGTGTGGGGTCTTTGATAACTATAGAGCTGCATCTAGGTTGGTTTAGTAACAAGTGGATGGGATTGGCTTGCTGTTTCGTTTTTAGACTCCTCAAACCACTTCCCCCTTCTGTTGCGTGGTACGTTGATTGCGAATTTAGGGCCAGTGGGGAACCCCAGTATTGCTTTAGTCCAGTGTTTGAGGAAGAATGGGGTCAACCTGTATTGGATCACATTTGGTTCTTCTATAAACCCCGTCATCAATTCTACTCAAAAAATAAGTGGCCAGATATCCACGGTCCTCTTGTATTCTCATTTCAACCTCGCTACTGGGATGGCGGAAAAATAATGAGTGAGGATACTGTGCAAGTGAAGAAGTGTGGGGTCCGTATGGTATATGAGGAAGATGTGGAGGAGCTTTGGGAAACACTATTGAAGCAGCACAATGTTCGCATTAATACCAAACGAGGCCTTCAACACTATGATGACGATGATGTTGCAGCATCTAGTAGTGCAAATTTGGCTGATCCAAAAAGAATTAAACATCACCACCTTGACAGCGCAGGACCCAATGGAATCCCTTATATTCATGACCAGAAACATGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006349 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010468 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019222 GO:0022414 GO:0032259 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043076 GO:0043078 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0060255 GO:0061458 GO:0065007 GO:0071514 GO:0071704 GO:0090304 GO:0140097 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Perm-CXXC RRM_DME

Protein Analysis

1088

Amino Acids

124.24

Weight (kDa)

6.59

Isoelectric Point (pI)

41.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	12 - 178	3e-51	TIR domain
TIR_2	PF13676	15 - 111	1e-12	TIR domain
NB-ARC	PF00931	169 - 335	2.1e-22	NB-ARC domain
WHD_ROQ1	PF23282	358 - 407	2.4e-10	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	565 - 652	1.1e-08	Leucine-rich repeat region
LRR_14	PF23598	629 - 714	5.3e-12	Leucine-rich repeat region
C-JID	PF20160	883 - 1024	9.4e-15	C-JID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0002428)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04560 AT5G04560 AT5G04560
fragaria_vesca	FvH4_2g10170 FvH4_2g10170 FvH4_2g10170 FvH4_2g10170 FvH4_2g10170
malus_domestica	MD02G1245300.v1.1 MD07G1071800.v1.1 MD09G1259000.v1.1 MD09G1259100.v1.1 MD17G1253500.v1.1 MD17G1253600.v1.1
prunus_persica	Prupe.2G089400_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1 Prupe.7G005000_v2.0.a1
pyrus_communis	pycom02g21110 pycom09g17380 pycom17g25620 pycom17g25630
rosa_chinensis	RchiOBHm_Chr6g0267351 RchiOBHm_Chr6g0267361
rosa_laevigata	RLG00000014013
rosa_multiflora	Rmu_sc0000746.1_g000002 Rmu_sc0019664.1_g000001
rosa_roxburghii	Rroxscaffold_7G00200830
rosa_rugosa	Rorug06G0031600 Rorug06G0031700 Rorug06G0031800 Rorug06G0031900 Rorug06G0032000
rosa_samantha	Rh6AG153500 Rh6BG153200 Rh6CG149300 Rh6DG137600
rosa_wichuraiana	Rw6G013200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	552
Acc36I	ACCTGC	1 cut(s)	1425
AccB7I	CCANNNNNTGG	2 cut(s)	1192, 2683
AccBSI	CCGCTC	1 cut(s)	608
AccI	GTMKAC	3 cut(s)	688, 1344, 1859
AccII	CGCG	1 cut(s)	1212
AciI	CCGC	8 cut(s)	553, 579, 608, 1210, 1212, 2195, 2590, 2997
AclWI	GGATC	6 cut(s)	134, 320, 2240, 2253, 2895, 3179
AcoI	YGGCCR	3 cut(s)	2362, 2481, 2942
AcuI	CTGAAG	5 cut(s)	459, 1038, 1418, 1884, 2289
AcvI	CACGTG	1 cut(s)	1794
AcyI	GRCGYC	1 cut(s)	549
AfaI	GTAC	5 cut(s)	339, 1083, 1683, 2669, 2801
AfiI	CCNNNNNNNGG	5 cut(s)	758, 774, 1192, 1423, 2683
AflII	CTTAAG	3 cut(s)	692, 977, 1756
AflIII	ACRYGT	4 cut(s)	1273, 1552, 1791, 1793
AleI	CACNNNNGTG	1 cut(s)	2679
AloI	GAACNNNNNNTCC	2 cut(s)	1107, 1139
Alw21I	GWGCWC	2 cut(s)	8, 17
Alw26I	GTCTC	2 cut(s)	1539, 1766
AlwI	GGATC	6 cut(s)	134, 320, 2240, 2253, 2895, 3179
AlwNI	CAGNNNCTG	1 cut(s)	1516
AoxI	GGCC	6 cut(s)	735, 2362, 2481, 2820, 2942, 3127
ApeKI	GCWGC	6 cut(s)	185, 304, 1516, 2707, 3097, 3158
ArsI	GACNNNNNNTTYG	2 cut(s)	2167, 2199
AseI	ATTAAT	2 cut(s)	1596, 3114
Asp700I	GAANNNNTTC	4 cut(s)	998, 1026, 1437, 2628
AspLEI	GCGC	1 cut(s)	3221
AspS9I	GGNCC	5 cut(s)	2009, 2820, 2957, 3043, 3224
AsuC2I	CCSGG	1 cut(s)	1233
AsuHPI	GGTGA	5 cut(s)	12, 374, 2121, 2666, 3197
AsuNHI	GCTAGC	1 cut(s)	2453
AvaII	GGWCC	4 cut(s)	2009, 2957, 3043, 3224
AxyI	CCTNAGG	1 cut(s)	1422
BaeGI	GKGCMC	1 cut(s)	2067
BalI	TGGCCA	2 cut(s)	2483, 2944
BamHI	GGATCC	1 cut(s)	2245
BanII	GRGCYC	1 cut(s)	17
BauI	CACGAG	1 cut(s)	627
BbrPI	CACGTG	1 cut(s)	1794
BbsI	GAAGAC	4 cut(s)	1168, 1913, 2277, 2295
Bbv12I	GWGCWC	2 cut(s)	8, 17
BbvI	GCAGC	6 cut(s)	172, 291, 1503, 2694, 3109, 3170
BccI	CCATC	7 cut(s)	99, 834, 1191, 1501, 2440, 2731, 2987
BcgI	CGANNNNNNTGC	6 cut(s)	1085, 1119, 3089, 3123, 3137, 3171
BciVI	GTATCC	3 cut(s)	61, 1485, 3010
BcnI	CCSGG	1 cut(s)	1233
BcoDI	GTCTC	2 cut(s)	1539, 1766
BfaI	CTAG	7 cut(s)	900, 1526, 2373, 2423, 2454, 2714, 3165
BfmI	CTRYAG	1 cut(s)	2700
BfrI	CTTAAG	3 cut(s)	692, 977, 1756
BfuAI	ACCTGC	1 cut(s)	1425
BfuI	GTATCC	3 cut(s)	61, 1485, 3010
BglII	AGATCT	1 cut(s)	1136
BisI	GCNGC	6 cut(s)	186, 305, 1517, 2708, 3098, 3159
BlsI	GCNGC	6 cut(s)	187, 306, 1518, 2709, 3099, 3160
BmcAI	AGTACT	2 cut(s)	1083, 1683
Bme1390I	CCNGG	1 cut(s)	1233
Bme18I	GGWCC	4 cut(s)	2009, 2957, 3043, 3224
BmgT120I	GGNCC	5 cut(s)	2009, 2820, 2957, 3043, 3224
BmiI	GGNNCC	8 cut(s)	763, 1415, 1572, 2010, 2247, 2832, 3044, 3226
BmrFI	CCNGG	1 cut(s)	1233
BmrI	ACTGGG	3 cut(s)	2277, 2830, 2998
BmsI	GCATC	5 cut(s)	245, 1161, 2285, 2719, 3170
BmtI	GCTAGC	1 cut(s)	2457
BmuI	ACTGGG	3 cut(s)	2277, 2830, 2998
BoxI	GACNNNNGTC	1 cut(s)	1794
BpiI	GAAGAC	4 cut(s)	1168, 1913, 2277, 2295
BplI	GAGNNNNNCTC	2 cut(s)	610, 642
BpmI	CTGGAG	1 cut(s)	156
BpuEI	CTTGAG	1 cut(s)	2618
BpuMI	CCSGG	1 cut(s)	1233
BsaAI	YACGTR	1 cut(s)	1794
BsaBI	GATNNNNATC	1 cut(s)	324
BsaHI	GRCGYC	1 cut(s)	549
BsaJI	CCNNGG	3 cut(s)	26, 1210, 2953
BsaXI	ACNNNNNCTCC	4 cut(s)	346, 376, 398, 428
Bsc4I	CCNNNNNNNGG	5 cut(s)	758, 774, 1192, 1423, 2683
Bse1I	ACTGG	9 cut(s)	869, 1297, 1840, 2053, 2283, 2823, 2836, 2852, 2993
Bse21I	CCTNAGG	1 cut(s)	1422
Bse3DI	GCAATG	2 cut(s)	2283, 2579
Bse8I	GATNNNNATC	1 cut(s)	324
BseDI	CCNNGG	3 cut(s)	26, 1210, 2953
BseJI	GATNNNNATC	1 cut(s)	324
BseLI	CCNNNNNNNGG	5 cut(s)	758, 774, 1192, 1423, 2683
BseMI	GCAATG	2 cut(s)	2283, 2579
BseMII	CTCAG	4 cut(s)	1523, 1592, 2338, 2343
BseNI	ACTGG	9 cut(s)	869, 1297, 1840, 2053, 2283, 2823, 2836, 2852, 2993
BseRI	GAGGAG	5 cut(s)	15, 1829, 2059, 2759, 3086
BseSI	GKGCMC	1 cut(s)	2067
BseXI	GCAGC	6 cut(s)	172, 291, 1503, 2694, 3109, 3170
Bsh1236I	CGCG	1 cut(s)	1212
BshFI	GGCC	6 cut(s)	737, 2364, 2483, 2822, 2944, 3129
BsiHKAI	GWGCWC	2 cut(s)	8, 17
BsiSI	CCGG	1 cut(s)	1232
BslFI	GGGAC	1 cut(s)	1440
BslI	CCNNNNNNNGG	5 cut(s)	758, 774, 1192, 1423, 2683
BsmAI	GTCTC	2 cut(s)	1539, 1766
BsmFI	GGGAC	1 cut(s)	1440
BsmI	GAATGC	4 cut(s)	284, 998, 1000, 2227
BsnI	GGCC	6 cut(s)	737, 2364, 2483, 2822, 2944, 3129
Bsp1286I	GDGCHC	3 cut(s)	8, 17, 2067
Bsp143I	GATC	7 cut(s)	139, 325, 1066, 1136, 2245, 2887, 3184
BspACI	CCGC	8 cut(s)	553, 579, 608, 1210, 1212, 2195, 2590, 2997
BspANI	GGCC	6 cut(s)	737, 2364, 2483, 2822, 2944, 3129
BspCNI	CTCAG	4 cut(s)	1522, 1593, 2337, 2344
BspFNI	CGCG	1 cut(s)	1212
BspHI	TCATGA	3 cut(s)	379, 754, 3247
BspLI	GGNNCC	8 cut(s)	763, 1415, 1572, 2010, 2247, 2832, 3044, 3226
BspMI	ACCTGC	1 cut(s)	1425
BspOI	GCTAGC	1 cut(s)	2457
BspPI	GGATC	6 cut(s)	134, 320, 2240, 2253, 2895, 3179
BspQI	GCTCTTC	1 cut(s)	22
BspTI	CTTAAG	3 cut(s)	692, 977, 1756
BsrBI	CCGCTC	1 cut(s)	608
BsrDI	GCAATG	2 cut(s)	2283, 2579
BsrI	ACTGG	9 cut(s)	869, 1297, 1840, 2053, 2283, 2823, 2836, 2852, 2993
BssECI	CCNNGG	3 cut(s)	26, 1210, 2953
BssMI	GATC	7 cut(s)	139, 325, 1066, 1136, 2245, 2887, 3184
BssNI	GRCGYC	1 cut(s)	549
BssSI	CACGAG	1 cut(s)	627
Bst2BI	CACGAG	1 cut(s)	627
Bst4CI	ACNGT	8 cut(s)	301, 1091, 1150, 1699, 1885, 2127, 2957, 3022
Bst6I	CTCTTC	1 cut(s)	22
BstACI	GRCGYC	1 cut(s)	549
BstAFI	CTTAAG	3 cut(s)	692, 977, 1756
BstAPI	GCANNNNNTGC	2 cut(s)	182, 1180
BstBAI	YACGTR	1 cut(s)	1794
BstC8I	GCNNGC	4 cut(s)	1741, 2408, 2455, 2749
BstDEI	CTNAG	6 cut(s)	1166, 1422, 1509, 1601, 2324, 2352
BstDSI	CCRYGG	2 cut(s)	1210, 2953
BstEII	GGTNACC	2 cut(s)	1010, 2127
BstENI	CCTNNNNNAGG	3 cut(s)	756, 772, 1421
BstFNI	CGCG	1 cut(s)	1212
BstHHI	GCGC	1 cut(s)	3221
BstKTI	GATC	7 cut(s)	142, 328, 1069, 1139, 2248, 2890, 3187
BstMAI	GTCTC	2 cut(s)	1539, 1766
BstMBI	GATC	7 cut(s)	139, 325, 1066, 1136, 2245, 2887, 3184
BstMWI	GCNNNNNNNGC	5 cut(s)	12, 182, 614, 1180, 3179
BstNSI	RCATGY	2 cut(s)	1277, 1556
BstPAI	GACNNNNGTC	1 cut(s)	1794
BstPI	GGTNACC	2 cut(s)	1010, 2127
BstSCI	CCNGG	1 cut(s)	1231
BstSFI	CTRYAG	1 cut(s)	2700
BstSLI	GKGCMC	1 cut(s)	2067
BstUI	CGCG	1 cut(s)	1212
BstV1I	GCAGC	6 cut(s)	172, 291, 1503, 2694, 3109, 3170
BstV2I	GAAGAC	4 cut(s)	1168, 1913, 2277, 2295
BstX2I	RGATCY	2 cut(s)	1136, 2245
BstXI	CCANNNNNNTGG	1 cut(s)	3260
BstYI	RGATCY	2 cut(s)	1136, 2245
Bsu36I	CCTNAGG	1 cut(s)	1422
BsuI	GTATCC	3 cut(s)	61, 1485, 3010
BsuRI	GGCC	6 cut(s)	737, 2364, 2483, 2822, 2944, 3129
BtgI	CCRYGG	2 cut(s)	1210, 2953
BtgZI	GCGATG	1 cut(s)	411
BtsI	GCAGTG	3 cut(s)	99, 1750, 1936
BtsIMutI	CAGTG	7 cut(s)	99, 1750, 1833, 1936, 2290, 2830, 2859
BveI	ACCTGC	1 cut(s)	1425
Cac8I	GCNNGC	4 cut(s)	1741, 2408, 2455, 2749
CaiI	CAGNNNCTG	1 cut(s)	1516
CciI	TCATGA	3 cut(s)	379, 754, 3247
CfoI	GCGC	1 cut(s)	3221
Cfr13I	GGNCC	5 cut(s)	2009, 2820, 2957, 3043, 3224
Cfr42I	CCGCGG	1 cut(s)	1213
CseI	GACGC	3 cut(s)	1907, 2282, 2347
Csp6I	GTAC	5 cut(s)	338, 1082, 1682, 2668, 2800
CviQI	GTAC	5 cut(s)	338, 1082, 1682, 2668, 2800
DdeI	CTNAG	6 cut(s)	1166, 1422, 1509, 1601, 2324, 2352
DpnI	GATC	7 cut(s)	141, 327, 1068, 1138, 2247, 2889, 3186
DpnII	GATC	7 cut(s)	139, 325, 1066, 1136, 2245, 2887, 3184
DraI	TTTAAA	2 cut(s)	840, 1639
EaeI	YGGCCR	3 cut(s)	2362, 2481, 2942
Eam1104I	CTCTTC	1 cut(s)	22
EarI	CTCTTC	1 cut(s)	22
EciI	GGCGGA	1 cut(s)	3012
Ecl136II	GAGCTC	1 cut(s)	15
Eco147I	AGGCCT	1 cut(s)	3129
Eco24I	GRGCYC	1 cut(s)	17
Eco32I	GATATC	1 cut(s)	2950
Eco47I	GGWCC	4 cut(s)	2009, 2957, 3043, 3224
Eco53kI	GAGCTC	1 cut(s)	15
Eco57I	CTGAAG	5 cut(s)	459, 1038, 1418, 1884, 2289
Eco72I	CACGTG	1 cut(s)	1794
Eco81I	CCTNAGG	1 cut(s)	1422
Eco91I	GGTNACC	2 cut(s)	1010, 2127
EcoICRI	GAGCTC	1 cut(s)	15
EcoNI	CCTNNNNNAGG	3 cut(s)	756, 772, 1421
EcoO109I	RGGNCCY	2 cut(s)	2009, 3224
EcoO65I	GGTNACC	2 cut(s)	1010, 2127
EcoRI	GAATTC	1 cut(s)	463
EcoRV	GATATC	1 cut(s)	2950
EcoT22I	ATGCAT	3 cut(s)	238, 284, 1000
EcoT38I	GRGCYC	1 cut(s)	17
FalI	AAGNNNNNCTT	4 cut(s)	725, 757, 1014, 1046
FaqI	GGGAC	1 cut(s)	1440
FauI	CCCGC	4 cut(s)	572, 1205, 2188, 2597
FblI	GTMKAC	3 cut(s)	688, 1344, 1859
Fnu4HI	GCNGC	6 cut(s)	186, 305, 1517, 2708, 3098, 3159
FriOI	GRGCYC	1 cut(s)	17
Fsp4HI	GCNGC	6 cut(s)	186, 305, 1517, 2708, 3098, 3159
FspBI	CTAG	7 cut(s)	900, 1526, 2373, 2423, 2454, 2714, 3165
GlaI	GCGC	1 cut(s)	3220
GluI	GCNGC	6 cut(s)	186, 305, 1517, 2708, 3098, 3159
GsuI	CTGGAG	1 cut(s)	156
HaeIII	GGCC	6 cut(s)	737, 2364, 2483, 2822, 2944, 3129
HapII	CCGG	1 cut(s)	1232
HgaI	GACGC	3 cut(s)	1907, 2282, 2347
HhaI	GCGC	1 cut(s)	3221
Hin1I	GRCGYC	1 cut(s)	549
Hin6I	GCGC	1 cut(s)	3219
HinP1I	GCGC	1 cut(s)	3219
HincII	GTYRAC	4 cut(s)	243, 1666, 1774, 2876
HindII	GTYRAC	4 cut(s)	243, 1666, 1774, 2876
HindIII	AAGCTT	3 cut(s)	980, 1982, 2030
HpaI	GTTAAC	1 cut(s)	1774
HpaII	CCGG	1 cut(s)	1232
HphI	GGTGA	5 cut(s)	12, 374, 2121, 2666, 3197
Hpy166II	GTNNAC	9 cut(s)	243, 689, 823, 1345, 1666, 1774, 1860, 2449, 2876
Hpy188I	TCNGA	8 cut(s)	748, 1362, 1512, 1560, 1602, 1827, 2269, 2617
Hpy8I	GTNNAC	9 cut(s)	243, 689, 823, 1345, 1666, 1774, 1860, 2449, 2876
Hpy99I	CGWCG	1 cut(s)	2363
HpyAV	CCTTC	9 cut(s)	530, 659, 727, 752, 940, 1304, 2514, 2796, 3140
HpyCH4III	ACNGT	8 cut(s)	301, 1091, 1150, 1699, 1885, 2127, 2957, 3022
HpyCH4IV	ACGT	5 cut(s)	549, 1266, 1793, 1856, 2802
HpyF10VI	GCNNNNNNNGC	5 cut(s)	12, 182, 614, 1180, 3179
HpyF3I	CTNAG	6 cut(s)	1166, 1422, 1509, 1601, 2324, 2352
HpySE526I	ACGT	5 cut(s)	549, 1266, 1793, 1856, 2802
Hsp92I	GRCGYC	1 cut(s)	549
HspAI	GCGC	1 cut(s)	3219
KspAI	GTTAAC	1 cut(s)	1774
KspI	CCGCGG	1 cut(s)	1213
Kzo9I	GATC	7 cut(s)	139, 325, 1066, 1136, 2245, 2887, 3184
LguI	GCTCTTC	1 cut(s)	22
LmnI	GCTCC	5 cut(s)	761, 1188, 1570, 1816, 3073
Lsp1109I	GCAGC	6 cut(s)	172, 291, 1503, 2694, 3109, 3170
LweI	GCATC	5 cut(s)	245, 1161, 2285, 2719, 3170
MaeI	CTAG	7 cut(s)	900, 1526, 2373, 2423, 2454, 2714, 3165
MaeII	ACGT	5 cut(s)	549, 1266, 1793, 1856, 2802
MaeIII	GTNAC	9 cut(s)	696, 788, 1010, 1072, 1354, 2127, 2183, 2252, 2726
MalI	GATC	7 cut(s)	141, 327, 1068, 1138, 2247, 2889, 3186
MbiI	CCGCTC	1 cut(s)	608
MboI	GATC	7 cut(s)	139, 325, 1066, 1136, 2245, 2887, 3184
MfeI	CAATTG	3 cut(s)	189, 1674, 1991
MflI	RGATCY	2 cut(s)	1136, 2245
MhlI	GDGCHC	3 cut(s)	8, 17, 2067
MlsI	TGGCCA	2 cut(s)	2483, 2944
MluNI	TGGCCA	2 cut(s)	2483, 2944
MlyI	GAGTC	4 cut(s)	1319, 1351, 1612, 2760
MmeI	TCCRAC	1 cut(s)	87
Mox20I	TGGCCA	2 cut(s)	2483, 2944
Mph1103I	ATGCAT	3 cut(s)	238, 284, 1000
MroXI	GAANNNNTTC	4 cut(s)	998, 1026, 1437, 2628
MscI	TGGCCA	2 cut(s)	2483, 2944
MslI	CAYNNNNRTG	3 cut(s)	1352, 2679, 3141
Msp20I	TGGCCA	2 cut(s)	2483, 2944
MspA1I	CMGCKG	1 cut(s)	1212
MspCI	CTTAAG	3 cut(s)	692, 977, 1756
MspI	CCGG	1 cut(s)	1232
MspR9I	CCNGG	1 cut(s)	1233
MunI	CAATTG	3 cut(s)	189, 1674, 1991
Mva1269I	GAATGC	4 cut(s)	284, 998, 1000, 2227
MvnI	CGCG	1 cut(s)	1212
MwoI	GCNNNNNNNGC	5 cut(s)	12, 182, 614, 1180, 3179
NciI	CCSGG	1 cut(s)	1233
NdeII	GATC	7 cut(s)	139, 325, 1066, 1136, 2245, 2887, 3184
NheI	GCTAGC	1 cut(s)	2453
NlaIV	GGNNCC	8 cut(s)	763, 1415, 1572, 2010, 2247, 2832, 3044, 3226
NmuCI	GTSAC	5 cut(s)	788, 1354, 2127, 2183, 2252
NsiI	ATGCAT	3 cut(s)	238, 284, 1000
NspI	RCATGY	2 cut(s)	1277, 1556
OliI	CACNNNNGTG	1 cut(s)	2679
PagI	TCATGA	3 cut(s)	379, 754, 3247
PceI	AGGCCT	1 cut(s)	3129
PciI	ACATGT	2 cut(s)	1273, 1552
PciSI	GCTCTTC	1 cut(s)	22
PcsI	WCGNNNNNNNCGW	2 cut(s)	1475, 2808
PctI	GAATGC	4 cut(s)	284, 998, 1000, 2227
PdmI	GAANNNNTTC	4 cut(s)	998, 1026, 1437, 2628
PflMI	CCANNNNNTGG	2 cut(s)	1192, 2683
PkrI	GCNGC	6 cut(s)	187, 306, 1518, 2709, 3099, 3160
PleI	GAGTC	4 cut(s)	1319, 1351, 1611, 2760
PmaCI	CACGTG	1 cut(s)	1794
PmlI	CACGTG	1 cut(s)	1794
PpsI	GAGTC	4 cut(s)	1319, 1351, 1611, 2760
Ppu21I	YACGTR	1 cut(s)	1794
PpuMI	RGGWCCY	2 cut(s)	2009, 3224
PscI	ACATGT	2 cut(s)	1273, 1552
PshAI	GACNNNNGTC	1 cut(s)	1794
PshBI	ATTAAT	2 cut(s)	1596, 3114
Psp124BI	GAGCTC	1 cut(s)	17
Psp5II	RGGWCCY	2 cut(s)	2009, 3224
PspCI	CACGTG	1 cut(s)	1794
PspEI	GGTNACC	2 cut(s)	1010, 2127
PspN4I	GGNNCC	8 cut(s)	763, 1415, 1572, 2010, 2247, 2832, 3044, 3226
PspPI	GGNCC	5 cut(s)	2009, 2820, 2957, 3043, 3224
PspPPI	RGGWCCY	2 cut(s)	2009, 3224
PstNI	CAGNNNCTG	1 cut(s)	1516
PsuI	RGATCY	2 cut(s)	1136, 2245
RsaI	GTAC	5 cut(s)	339, 1083, 1683, 2669, 2801
RsaNI	GTAC	5 cut(s)	338, 1082, 1682, 2668, 2800
RseI	CAYNNNNRTG	3 cut(s)	1352, 2679, 3141
SacI	GAGCTC	1 cut(s)	17
SacII	CCGCGG	1 cut(s)	1213
SapI	GCTCTTC	1 cut(s)	22
SatI	GCNGC	6 cut(s)	186, 305, 1517, 2708, 3098, 3159
Sau3AI	GATC	7 cut(s)	139, 325, 1066, 1136, 2245, 2887, 3184
Sau96I	GGNCC	5 cut(s)	2009, 2820, 2957, 3043, 3224
ScaI	AGTACT	2 cut(s)	1083, 1683
SchI	GAGTC	4 cut(s)	1319, 1351, 1612, 2760
ScrFI	CCNGG	1 cut(s)	1233
SduI	GDGCHC	3 cut(s)	8, 17, 2067
SfaNI	GCATC	5 cut(s)	245, 1161, 2285, 2719, 3170
SfcI	CTRYAG	1 cut(s)	2700
Sfr303I	CCGCGG	1 cut(s)	1213
SgrBI	CCGCGG	1 cut(s)	1213
SinI	GGWCC	4 cut(s)	2009, 2957, 3043, 3224
SmiMI	CAYNNNNRTG	3 cut(s)	1352, 2679, 3141
SmlI	CTYRAG	4 cut(s)	692, 977, 1756, 2597
SmoI	CTYRAG	4 cut(s)	692, 977, 1756, 2597
SseBI	AGGCCT	1 cut(s)	3129
SsiI	CCGC	8 cut(s)	553, 579, 608, 1210, 1212, 2195, 2590, 2997
SspI	AATATT	1 cut(s)	845
SspMI	CTAG	7 cut(s)	900, 1526, 2373, 2423, 2454, 2714, 3165
SstI	GAGCTC	1 cut(s)	17
StuI	AGGCCT	1 cut(s)	3129
StyD4I	CCNGG	1 cut(s)	1231
TaaI	ACNGT	8 cut(s)	301, 1091, 1150, 1699, 1885, 2127, 2957, 3022
TaiI	ACGT	5 cut(s)	552, 1269, 1796, 1859, 2805
TaqI	TCGA	5 cut(s)	951, 1069, 1131, 1521, 2154
TatI	WGTACW	2 cut(s)	1081, 1681
TscAI	CASTG	7 cut(s)	106, 1750, 1840, 1936, 2290, 2830, 2859
TseFI	GTSAC	5 cut(s)	788, 1354, 2127, 2183, 2252
TseI	GCWGC	6 cut(s)	185, 304, 1516, 2707, 3097, 3158
Tsp45I	GTSAC	5 cut(s)	788, 1354, 2127, 2183, 2252
TspGWI	ACGGA	4 cut(s)	661, 1467, 1583, 3035
TspRI	CASTG	7 cut(s)	106, 1750, 1840, 1936, 2290, 2830, 2859
Van91I	CCANNNNNTGG	2 cut(s)	1192, 2683
Vha464I	CTTAAG	3 cut(s)	692, 977, 1756
VpaK11BI	GGWCC	4 cut(s)	2009, 2957, 3043, 3224
VspI	ATTAAT	2 cut(s)	1596, 3114
XagI	CCTNNNNNAGG	3 cut(s)	756, 772, 1421
XbaI	TCTAGA	1 cut(s)	2372
XceI	RCATGY	2 cut(s)	1277, 1556
XcmI	CCANNNNNNNNNTGG	1 cut(s)	866
XmiI	GTMKAC	3 cut(s)	688, 1344, 1859
XmnI	GAANNNNTTC	4 cut(s)	998, 1026, 1437, 2628
XspI	CTAG	7 cut(s)	900, 1526, 2373, 2423, 2454, 2714, 3165
ZraI	GACGTC	1 cut(s)	550
ZrmI	AGTACT	2 cut(s)	1083, 1683
Zsp2I	ATGCAT	3 cut(s)	238, 284, 1000

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.